400/2004 Sb.
DECREE
of 23 December 2003. June 2004,
amending the Decree of the Ministry of agriculture no. 275/1998 Coll., on
agrochemical testing of agricultural soils and soil properties of discovery
forest land, as amended by Decree No. 477/2000 Sb.
The Ministry of agriculture shall establish, in agreement with the Ministry of health
According to § 10 para. 1 and 8 and section 16 (a). d) of Act No. 156/1998 Coll., on
fertilisers, soil conditioners, herbal medicines
and substrates and agrochemical testing of agricultural land (law on
fertilisers), as amended by Act No 317/2004 Coll.:
Article. (I)
Decree No 277/1998 Coll., on agrochemical testing of agricultural land and
detection of soil properties of forest land, as amended by Decree No.
477/2000 is amended as follows:
1. in article 2, the following paragraph 5 is added:
"(5) in the cases provided for by law about fertilizers agro-chemical testing
agricultural soils also includes microbiological and physical analyses. ".
2. In paragraph 3, the following paragraphs 4 and 5 are added:
"(4) the principles of microbiological analyses of agricultural land and the criteria for
evaluation of their results are listed in annex 7.
(5) principles of physical analyses of agricultural land and the criteria for
evaluation of their results are given in annex 8. ".
3. In annex 3, the following shall be added after point 3, paragraphs 4 to 6 shall be added:
"4. The determination of the carbon of oxygenated
Assay procedure: Oxygenated organic bound carbon in soil is
chromic acid oxidizes in the environment of excess sulphuric acid
defined conditions. Results expressed as% it is possible to recalculate the Cox
the% of humus, provided that humic acids contain 58% (C).
5. Determination of total nitrogen
Procedure for determining the soil sample is digested by wet concentrated
sulphuric acid in the presence of catalyst (Kjeldahl method),
which organic compounds are oxidized by N to NH4 +, which is after distillation
provides for the neutralization titration of the excess acid
the standard solution for the policy.
6. determination of the potential for cation exchange capacity
Assay procedure: Sorption complex soil with barium ions recurring
extraction of the barium chloride solution to a pH value of 8.1. In
the eluate is acidimetrickou by titration of hydrogen Exchange provides. In the second step
the sorbované barium from the sample displace magnesium chloride solution. In
the second of the eluate down barium, whose concentration is directly proportional to the
the potential for cation exchange capacity. "
4. After annex No 6 to annex No. 7 and 8 are added:
' Annex # 7 to Decree No 277/1998 Coll.
Microbiological analyses of agricultural land
1. principles of microbial analyses of agricultural land
Determining the abundance and soil microflora activity measurement of respiratory
curves (ISO DIS 17155)
Fresh soil samples with a water content corresponding to 40-60% of maximum
water holding capacity are before you start measuring preinkubovány 3-4 days when
constant temperature. The recommended temperature is in the range 20-25
degrees C During measurements at regular intervals (recommended every
an hour) was established as the carbon dioxide released or oxygen consumed.
The speed of the basal respiration is defined as the amount of CO2 released by the
or the O2 consumed per unit of time without the addition of substrate.
Respiratory curves are measured after addition of easily biodegradable
substrate (e.g., glucose). The measurement takes place, until there is a decrease in the
the speed of respiration. For the measurement of respiration can be applied to any device
to enable continuous measurement of evolved carbon dioxide or
consumed by oxygen.
From respiratory curves are subtracted the values of parameters characterizing the
biomass and activity of microbial communities: substrate induced
respiration, lag phase, growth speed and time tpeakmax. Substrate
We understand the constant losses by respiration induced increased respiration rate
immediately after addition of the substrate, the lag phase time to begin
the exponential growth the speed of respiration and the growth speed
speed constant measured during exponential growth rate
respiration. Tpeakmax time is the time from the addition of the substrate to a maximum
respiratory rate. Of the proportion of the basal and the substrate-induced
respiration is the calculated value of the activation of the respiratory quotient QR.
Nitrifying potential-a quick test by measuring the rate of oxidation of ammonium
ions (ISO DIS 15685)
The rate of oxidation of ammonium ions to destroy is measured within 6 hours
incubation in soil suspension buffered to pH 7.2. Substrate is a suspension
added in the form of ammonium sulphate. The oxidation of nitrite to nitrate is
inhibited by the addition of sodium chlorate. Destroy the ions are
set a suitable analytical method.
To determine whether the soil contains a contaminant that affects
nitrification, the control soil sample with a known value
nitrifikačního potential in the range of 500-800 ng N g-1 h-1. Samples
the test and control soils are preinkubovány two days when humidity
the corresponding water content at 60% of the maximum water capacity. It is then
ready to be pooled in a ratio of 1:1 (calculated on the dry soil).
Pooled sample and samples of soil test and inspection are incubated for 1 day
at a temperature of 20 ° C, and then specified in them nitrification potential.
2. Evaluation criteria
Determining the abundance and soil microflora activity measurement of respiratory
curves
Contaminated soil show higher values of QR and longer lag phase and the
tpeakmax. QR values > 0.3 or lag phase > 20 h and 50 h at tpeakmax > R
0.2-0.3 indicate contaminated soil.
Nitrifying potential-a quick test by measuring the rate of oxidation of ammonium
ions
Test the soil is considered contaminated when nitrifying
the potential of a collective specimen is less than 90% of the average value
nitrifying potential samples of the test and control of land laid down
separately.
As + 0.9 * SD < Avyp
As-mechanically assisted pooled sample activity
Aprum-activity, calculated as the average of the control and test of soil
SD – standard deviation.
Annex No. 8 to Decree No 277/1998 Coll.
Physical analyses of agricultural land
Determination of the composition of the zrnitostního.
Assay procedure: according to the size of the soil particles divided into shares
zrnitostních factions. The basic skeleton of the dělítkem jemnozemě is the dimension of the
2 mm, which was chosen because it is the upper limit of the capillary movement of
water. For the classification of zrnitostních fractions is most commonly used
sedimentation the pipetting method. Lies in depths from certain
sedimentující suspension in the cylinder after the sedimentačním of the respective
the times takes a small share, which after drying. The results of the
zrnitostního of analysis is usually adjusted to the table.
Evaluation criteria: For the purposes of the scale, the agronomic use
Earth by Novak, which graded the soil under the representation of the fraction below 0.01
mm.
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The content of the particles (grains) smaller land Classification indicate the type of soil
0.01 mm in%
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0-10 Sandy P light
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10-20 HP hlinitopísčitá light
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20-30 moderate PH písčitohlinitá
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30-45 aluminum H medium difficulty
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45-60 jílovitohlinitá JH heavy
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60-75 clay JV heavy
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over 75 clay (or prchlice) J heavy
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Determination of the content of the skeleton
Assay procedure: Water in the measuring cylinder down the volume of the sample, the
shall be distributed to the screens of the factions, the volume gradually lays down
again in the measuring cylinder. The result-the volume of individual fractions are expressed
in% of the total volume of the sample. Evaluation criteria: classification scale,
created for a comprehensive survey of soil, divides the land of the jemnozem (under 2 mm) and
skeleton. It is made up of the three factions (coarse sand 2-4 mm, 4-gravel
30 mm, stones over 30 mm). Application range is suitable for larger samples
(the order of kg), taken exclusively for this determination.
The contents of the skeleton in the ornici to 20% usually will not adversely affect either the
plant growth, or in cultivation. The higher the volume goes to the detriment of jemnozemě and
particularly adversely affect the skeleton with a heavy clay layers of sealer.
Determination of the physical properties.
Assay procedure: physical properties means indicators,
discovered on the intact soil sample, taken to a metal ring.
The procedure is suitable for fine grained cohesive soil without crude skeleton
or large organic debris. In the sample, taken from the best of the probe,
identifies the weight of the fresh water saturated, expressed and dried
sample and determination of its apparent density will provide the basic data for
the calculation of the volumetric moisture content searching parameters: momentní (instant,
the original), maximum capillary water capacity, momentní (original)
airiness, the minimum air capacity, absorption power, density
reduced (dry land), porosity.
Evaluation criteria reflect the properties of the soil. Momentní humidity
(vol.%) and momentní (%) the airiness of depends on the specific
the situation and the climate. Maximum capillary water capacity (volume%)
the coarser the soils, the smaller. For fine-grained, vazkých and compact
soil is the larger (usually 20-40%). The minimum air capacity (volume%)
-below 10% in field soils and meadow soils below the 5%-prone to waterlogging,
If this is a permanent state-land is waterlogged, above 20% for the fields-soil
vysychavé, if this is a permanent state-land wizened. Absorption power (volume
%)-indicates the saturation of the entire column of soil. capillary pores. U
clay soil is higher than that of sandy. According to the dimensional weight dry
According to the porosity of the soil and we can evaluate the structural State of the approximately
humusového term of moderate and heavy soils (Kutílek, 1966):
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The structural State of the humusového dry bulk density Porosity%
the horizon of the soil g.cm-3
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excellent less than 1.2 more than 54
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for 1.2-1.4 46-54
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unsatisfactory 1.4-1.6 39-46
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nestrukturní 1.6-1.8 31-39
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Article II
The effectiveness of the
This Decree shall enter into force on 1 January 2000. in August 2004.
Minister:
Ing. Palas in r.
