Sampling Requirements And Methods Of Testing Seeds And Seedlings

Original Language Title: požadavky na odběr vzorků a metody zkoušení osiva a sadby

Read the untranslated law here: https://portal.gov.cz/app/zakony/download?idBiblio=73883&nr=61~2F2011~20Sb.&ft=txt

61/2011 Coll.
DECREE


Dated March 9, 2011

The requirements of the sampling procedures and methods of testing seeds and seedlings

Change: 410/2013 Coll.

The Ministry of Agriculture stipulates pursuant to § 3a par. 1, § 4 para. 15 point.
) And b), § 5 para. 9 point. h), § 6. 10 point. c) to f), § 19 para. 15
point. l) and § 22 paragraph. 10 point. b) Act no. 219/2003 Coll., on
marketing of seeds and planting material and amending some laws
(Act on the marketing of seeds and seedlings), as amended by Act no. 178/2006 Coll.
Law no. 299/2007 Coll., Act no. 96/2009 Coll. and Act no. 300/2009 Coll., (hereinafter
"Act")

§ 1
Introductory provisions


(1) This Decree implements the relevant regulations of the European Union ^ 1) and
regulates requirements for sampling procedures and methods of testing seeds and seedlings
.

(2) For the purposes of this Decree

A) sub-sample batch of seeds or seedlings collected from lots of seed or seed
at individual sampling operation,

B) the aggregate sample pattern formed sesypáním and mixing all
partial samples taken from one batch,

C) laboratory sample a sample of the prescribed minimum weight
listed in Annex no. 5, intended to be sent to the testing laboratory, made up either of
entire aggregate sample, or created by dividing the aggregate sample

D) Reserve specimen sample to be stored for possible further testing
prepared from the same bulk sample in the same way as
laboratory sample; Reserve each sample must be labeled
"reserve sample" with an "R"

E) inspection sample sample for re-assessed health
seed potatoes

F) adjustment, acts involving packaging, sealing and labeling the sample
or seed lots

G) official samplers employee of the Central Control and Testing Institute for Agriculture
entitled to subscribe
official sample or a natural person authorized by the Department of sampling according to § 17 para. 3
Act

H) official sample sample taken and adjusted with official samplers,

I) a pattern on a vegetative test sample intended for growing
test to establish the species and varietal purity and authenticity, condition, or
hybridity,

J) common sample is a sample drawn together and adjusted
supplier and the customer,

K) private sample pattern that does not comply to the official or common
sample

L) standard sample is a variety which is growing
examinations and laboratory tests of authenticity intended to compare,

M) control sample sample taken for the purpose of official samplers
quality control propagation material placed on the market or for the purpose
official surveillance in accordance with § 17 para. 12 of the Act

N) test sample, a sample of the weight specified in Annex no. 5 and
used to determine the admixture of other plant species in pieces

O) basic test sample, a sample of the weight specified in Annex
no. 5 and used to determine the purity of seed percentage

P) homogeneity uniformity seed seed lots in the context of natural variability
,

Q) the heterogeneity of the seed of disunity seed lots in which individual ingredients
characterizing the quality, not evenly distributed,

R) samples to determine the health status of seed potato tubers of
removed after the end of the vegetation contained in Annex no. 1

S) swatches packaging or official reserve sample

R) přehližitelem official employee of the Department of State authorized to
shows the seed crops or natural person authorized by the Institute for
implementation parades seed crops according to § 17 para. 2 of the Act.

§ 2

To check accompanying documents, lots of propagating material

[To § 4 para. 15 point. b) and § 19 para. 15 point. l) of the Act]

Before sampling samplers contractor shall submit the following documents:

A) completed and signed application for recognition of propagating material

B) evidence of crop certification and crop forecast,

C) records under § 7 para. 1 of the Act,

D) records used labels and possibly a protocol on disposal of labels
and on the forms provided for in Annex no. 2

E) completed data to the pattern book to the model shown in Annex no. 2

F) for seed potatoes filled with labels on the form pursuant to Annex No. 2.


Technological processes and methods of sampling and how to detect heterogeneity of the seed lot


[To § 3a par. 11, § 4 para. 15 point. a), § 6. 10 point. c) and d) of the Act
]

§ 3

(1) When sampling (hereinafter referred to as "sampling") seed does
acts so that the laboratory or mechanical analyzes and vegetation
testing was available representative sample and a reserve sample of
weight specified in Annex 5.

(2) Sampling for the purpose of issuing official documents is authorized to carry out only
samplers.

(3) Lot ready to sample must be homogeneous and must not exceed
mass specified in Annex no. 5 by more than 5%.
Larger quantity is divided into several separate parts.

(4) When sampling seeds and seedlings conducted as part of the certification,
control of production and circulation of seeds and seedlings and supervision of the Institute according to § 17 para. 12 of the Act
used technological procedures specified in Annex no. 1 .

(5) Technical aids for sampling are set out in Appendix 1.

§ 4

(1) Minimum weight of laboratory samples of seeds of each species
listed in Annex no. 5, the mixture is determined according to the type that has a mixed
highest percentages of weight.

(2) The minimum sample size of seed potatoes is given in Annex 5.

(3) The minimum sample size of seed planter onion and garlic seed is
listed in Annex 5.

(4) The minimum sample size vegetable seedlings is presented in Appendix.
First

(5) The minimum sample size for vegetation tests
is listed in Annex 5.

(6) The minimum sample size pelleted, inlay and
granular seed, osivových belts and osivových mats
is given in Annex 5.

(7) Maximum weight of a lot of propagating material set out in
Annex 5.

§ 5

(1) For seed lots of specialty varieties or hybrids whose
weight is equal to or less than 1% of the stated maximum weight
game may be the weight of the laboratory sample reduced to the level specified weight
Annex no. 5 for a specimen for analysis purity.
In this case, you can not perform the calculation of the admixture of other
plant species. This rule also applies to samples taken at
vegetation exam.

(2) In the case of hybrid varieties of vegetables first filial generation can be
minimum weight of the laboratory sample of seeds is reduced to a quarter
weight specified in Annex no. 5, the sample must have at least 5 g and consist
at least 400 seeds.

(3) Unless the laboratory sample of the prescribed weight
laboratory tests will be postponed until the time of delivery of a new sample of sufficient weight.
Exception is the case of non-weight under paragraphs 1 and 2. In this case
analysis may be performed on a sample of less weight or
lower number of seeds. The document will then conducted an analysis indicated
following remark: "Tested on a sample of reduced weight or number of seeds
lower than specified."

(4) To determine the moisture content of seeds has a minimum mass of the test sample


A) 100 g cereals except millet, legumes, maize and sorghum,

B) 50 g for other species
while the condition is that the sample must be completely filled and tightly closed
. In justified cases, such as the measurement of moisture on
electric hygrometer may require higher sample weight.

(5) To determine the level of erucic acid and glucosinolates in rape seed
have a minimum weight of 100 g of the test sample.

(6) For the test of authenticity electrophoresis is minimal
mass of the test sample of 100 grams of seed purity test
lot electrophoretic minimum mass of the test sample corresponds
sample size that is specified in Annex no. 5 column # 4. for
seed potatoes is to determine the identity of the variety
minimum sample size of 10 tubers and to determine the purity of the game 100 tubers. Sample application for
electrophoresis is shown in Appendix 2.

(7) To control additives, genetically modified varieties of seed are
collected three samples, one of which is intended for laboratory testing
second contractor keeps as a reserve sample, and the third is kept by the Institute
in case of repeated tests. The minimum weight of the sample corresponds
sample size that is specified in Annex no. 5 Table no. 1

Column no. 4. For seed potatoes shall proceed accordingly, samples are stored
so as to prevent spoilage. The sample weight was 25 kg.

§ 6

(1) Where in the seed lot while sampling any signs
heterogeneity in the documents or the appearance of individual sub-samples, sampling
a lot must be refused or stopped.
In case of doubt about the homogeneity of the game can be tested for heterogeneity
as described in Appendix 1.

(2) The seed lot must be stored so that each individual package
game, even when stored on pallets was without exerting undue effort
open to inspection and sampling. Otherwise
sampling can not be done.

(3) If the requirements specified in § 2-5 and paragraphs 1 and 2
performs samplers sampling in accordance with technological
procedure set out in Appendix 1.

§ 7

(1) Lot seedlings ready for sampling can be stored in bags, bulk bags
, box pallets or may be in bulk.
For bulk seed or bulk bags, which can not be removed
test sample after finishing, samplers removes partial
samples during sorting. In these cases, the contractor shall notify the date of commencement
sort samplers at least 24 hours in advance.
Packages bear an official label samplers and fuse; for bulk seed
label is attached to the accompanying document.

(2) Sampling of seed potatoes is carried out according to the procedures set out in Annex
No.1.

(3) Sampling jig onion and garlic planting is done according to the procedures
listed in Appendix 1.

(4) Sampling of vegetable seedlings to control the species and varieties
authenticity or purity or state of health is carried out according
procedures given in Appendix 1.

§ 8

(1) For official samples are used, these containers taken at the expense of the supplier
:

A) multi-ply paper sample is completely taped and glued bottom side
circuit and with the data prescribed in Annex no. 2 in order to avoid undesirable
specimen handling,

B) sample from the hermetic materials and airtight
test for moisture and to detect the presence of animal pests and
seed samples intentionally podsušených to be packaged in gas-tight containers

C) of unused bags so thick fabric, which prevents unwanted hair
soil and other debris samples intended for seed potatoes,

D) bags for seedlings of onion and garlic seed for breathability
packaging.

(2) Each official sample is marked, to the obvious link between
propagating vegetation, lots and sample. Official documents on the results of the analysis
Department issued or authorized person only on the basis of analysis of official
sample.

(3) Seals intended for sealing of official samples Department issued only
samplers, ensuring that prevent their unauthorized use
.

(4) Official samples must be properly concluded and samplers fitted
official fuse so as to avoid possible manipulation of the sample without
flagrant violation of official policy. Applications which are an integral part
samples, samplers or under the supervision
inserted into a separate envelope, which samplers sealed and labeled its stamp and signature
and attach them to the mass package.

(5) Samples of seeds are packaged so as to prevent damage during transport
. Samplers prepare samples for dispatch, seal the container,
when sending mail can be sealed and marked with a stamp and signature.
Samples in multilayer paper bags to be sent by post
, samplers individually inserted into canvas bags and seal the entire contents
.

(6) Laboratory samples must be no delay on the supplier's costs
sent for laboratory examination.

§ 9

(1) The provisions of § 8. 1 point. a), c) and d) and paragraphs 2-5
applies also for samples to test vegetation.

(2) Samples for testing vegetation sends the supplier without delay to
test station according to the instructions of the Institute. Specimen labels to sample the vegetation
test laid down in Annex No. 2.

(3) Samples for determining the health status of seed potatoes or
mechanical analysis of seed potatoes to be sent to the supplier at the place designated
Institute. Specimen labels sample to determine the health status of seedlings

Potatoes or for mechanical analysis of seed potatoes and the pattern of the label sample
seed potatoes to other countries of origin laid down in Annex No. 2.

§ 10

(1) when receiving the incoming samples are checked

A) identification of samplers and packaging

B) the nature of the seal or seals, their integrity and the integrity of the packaging
sample, samples common to describe the type of closure and

C) compliance data, in particular batch number on the bag and on the accompanying document
and completeness of the information on the document.

(2) If, when receiving the incoming samples of non-compliance by
paragraph 1, this fact is notified promptly suppliers.

(3) When defects are detected by

A) paragraph 1 point. a) and c) the sample is tested after the removal of these defects
,

B) of paragraph 1 point. b) the sample is not accepted for testing, and the contractor must apply for a new
sampling.

(4) of the sample taken shall be assigned a registration number, which simultaneously
assays indicated on the card or another document for testing. These documents
accompanies the sample throughout the tests.

(5) After testing laboratory samples of seeds and seed samples to test
growing stored at least until the next growing season
particular crop. Storage conditions are such that
not reduce the quality of the seed samples. If necessary, some
samples prematurely liquidate their disposal shall be recorded in the appropriate register
samples.

(6) The reserve sample of the seeds are stored in place and under the conditions specified
Institute for 2 years, if not on the label of seed put into circulation
otherwise specified.

(7) Samples of seed potatoes, including vegetation samples for testing, the
disposed of after the completion of the prescribed tests.

Procedures and methods of seed crops, testing
propagating material samples for the comparative trials and tests
within the European Union

[To § 5 para. 9 point. h), § 6. 10 point. f) e) and § 22 paragraph. 10
point. b) of the Act]

§ 11

(1) Shows seed crops přehlížitel carried out in accordance with
procedure set out in Appendix 3.

(2) unsuitable if the crop its properties
conditions laid down for the category referred to in the application for recognition, it can recognize
in a lower category or degree, at the request of the contractor in accordance with the procedure referred
in Appendix 3.

(3) Vegetation Institute tests conducted in accordance with the rules set out in Annex
. 4. Growing Tests are used

A) to check the varietal and species identity and purity,

B) to determine the percentage hybridity,

C) to check the health status of seed or seed potatoes

D) to determine the percentage of occurrence of weed beets in the beet seed and fodder beet
,

E) for subsequent inspection of standard seed of vegetables and vegetable seedlings,

F) as input and output tests within the seed certification system
by the Organization for Economic Cooperation and Development

G) as a possible part of the complaint procedure,

H) to control the production and marketing of propagating material into circulation

I) to monitor selected parts of propagating material imported from third countries
,

J) to check samples taken during inspection activities of persons responsible
sampling of propagating material or to perform shows
seed crops,

K) to control the officially unregistered varieties.

(4) The rules for establishing and assessing vegetation testing
are listed in Annex 4. Department draw up a protocol of assessment vegetation
tests. Model report of the assessment tests and vegetation pattern
sample labels for the growing test are listed in Appendix 2.

§ 12

(1) Samples for moisture determination and samples to determine the pests
tested immediately upon receipt at the laboratory and after analyzing the
liquidated. Other samples are tested progressively and without delay.

(2) In laboratory testing seeds and seedlings shall be governed by the applicable
testing methodology seeds and seedlings. The requirements are listed and published in the Bulletin of the Ministry of Agriculture
. Procedures that are in the methodology
testing seeds and seedlings mentioned are compatible with the methods
issued by the International Association for seed testing (ISTA), if for some
determining such methods exist.

(3) Requirements for sampling seeds and seedlings for the purpose of detecting the occurrence
GM and the procedures and methods for the detection of

Genetic modifications in samples of seeds and seedlings are given and published in
valid methodology for testing seeds and seedlings in the Bulletin of the Ministry of Agriculture
.

(4) Evidence of quality seeds and seedlings will be issued after completion of the tests laid down
for the type of seed or seedlings.

§ 13

(1) Samples for follow-up
propagating material of agricultural, vegetable and fruit crops, vines and vegetable seedlings
varieties of species included in the list of species and ornamental species that
carried out by the comparative tests and experiments within the countries of the European Union
is taken from propagating material

A) originating in third countries and

B) suitable for organic farming and

C) put in circulation to preserve genetic diversity.

(2) Tests and trials referred to in paragraph 1 are designed to control
compliance with the conditions under which the seeds and seedlings marketed, and
harmonization of methods of testing and certification. For
propagating material of fruit and ornamental species and vegetable seedlings are checked
well as compliance with phytosanitary regulations.

(3) Sampling is performed in accordance with § 3 to 7

(4) sampling, experiments and tests referred to in paragraphs 1-3 performs
Department.

§ 14


Transitional provisions
When sampling and testing of samples of seeds and seedlings, which was launched
prior to the effective date of this Decree, shall be governed by the existing legislation
.

§ 15
Repealing provisions


Repealed:

First Decree no. 206/2004 Coll., Laying down the requirements for taking
sampling procedures and methods of testing seeds and seedlings.

Second Decree no. 581/2006 Coll., Amending Decree no. 206/2004 Coll.
Laying down the requirements for sampling procedures and methods of testing
seeds and seedlings.

§ 16
Efficiency


This decree comes into force on 15 March 2011.
Minister
:

Ing. Fuksa vr
Appendix 1


Technological processes and methods of sampling of seeds and planting material and process
at finding heterogeneity of the seed lot

First Technical aids

(1) sampling instrument or device (Spike vzorkovadlo, double skin
vzorkovadlo, automatic vzorkovadlo)

(2) dělidlo,

(3) bucket and shovel to mix the sample

(4) scales,

(5) quilting needle, string,

(6) sample (multi-layer paper bag)

(7) an airtight container (eg. PE or PP bottle) for collecting seed
moisture determination, or detection of warehouse pests,

(8) cloth or other breathable pouch

(9) work surface (table) for adjustment of sample and administrative
activities during the sampling.

(10) new breathable packaging for samples of seed potatoes.

Second News subsamples

(1) The number or size of primary samples must samplers
chosen so as to meet the requirement of minimum sampling intensity
under Part 3, and also to be able to send to the lab
minimum amount of seed required for desired tests and creating a reserve
sample.

(2) The sampling session individual sub-samples
approximately the same size no matter from which place parts or packaging
samples are taken.

(3) If the seed lot in packages, containers are selected by a sampling of
whole lot, and it randomly or according to a predetermined plan.
Sub-samples are taken from the top, middle and bottom of the packaging, but not necessarily
from more than one place of a single package, if it is not necessary
to meet the minimum stocking sampling by Section 3

(4) If the seed lots in bulk containers or in bulk,
collecting sub-samples from randomly selected sites.

(5) Containers are the collection of subsamples open or pierce.
Packaging of which has been taken subsample is then closed or
contents transferred to new containers.

(6) To be a seed lot packed into special types of packaging (eg
small packages, the moisture packaging impenetrable packaging), collecting partial
samples, if possible, before or during the performance packaging.

(7) When sampling seed lots osivových in the form of strips or mats
taken the whole package or parts of strips or mats.

(8) Tools used in sampling should not harm the seeds or the

Not choose based on their size, shape, density,
osinatosti or any other property. All sampling tools
must be clean before sampling to avoid possible contamination.
Vzorkovadla must be long enough to close the aperture
pointed ends reach at least the middle of the container. If no container is accessible from both opposite sides
must vzorkovadlo long enough to achieve
opposing sides of the package. When sampling seed lots applying one of the methods below
.

2.1 Methods for subsamples

2.1.1. Spike (Nobbeho) vzorkovadlo

(1) consists of a tube with an opening at the pointed end.
Seed passes through the tube and collects into a suitable container. For cereals, the minimum inner diameter
vzorkovadla approximately 14 mm for larger seeds (maize)
the minimum inner diameter of approximately 20 mm vzorkovadla for
clover and similar seeds, the minimum inner diameter of approximately 10 vzorkovadla
mm. Outside the maximum diameter vzorkovadla
five times the maximum diameter of the sampled seeds. The width of the opening in the shell
vzorkovadla is at least twice the maximum diameter of sampled
seeds or impurities that may be present in the hand, the length of the hole
is three to four times the width of the opening.

(2) In use vzorkovadlo with an opening facing downward is inserted into the seed
angle of about 30 ° to the horizontal plane. Vzorkovadlem
inserted to a desired depth is rotated about the longitudinal axis 180 and
then drawn out of the container constantly decreasing rate while it
gently shaking in order to maintain an even flow of seed. Seed is collected in a suitable container
.

2.1.2. Bunded vzorkovadlo

(1) consists of an inner tube which loosely fits into the interior of the outer tube
, yet close soon enough that the gap between them can not slip
seeds or dirt. The outer tube has a solid pointed
end. The walls of the two tubes are cut holes, so that the cavity
inner tube can be opened and closed by turning the two tubes against each
other. Recommended dimensions vzorkovadla are: length vzorkovadla
such that vzorkovadlo reach the opposite wall of the packaging, if
vertical sampling bags must double shell vzorkovadlo
reach the bottom of the bags, the minimum internal diameter 25 mm, width of opening is at least || | twice the maximum diameter of sampled seeds or impurities which
can occur in batch, the length of the opening is at least twice the width of the opening
, the maximum length of the opening is not provided, the opening may be
as long as the entire vzorkovadlo.

(2) Bunded vzorkovadlo can be used in horizontal position, vertical or oblique
. Double shell vzorkovadlo with holes arranged in a spiral
which open gradually from the tip of the handle can be used only for
seeds is less than the seed of the species Triticum aestivum.

(3) When double-walled vzorkovadlo used vertically must either
bin internally dividing vzorkovadla several chambers or
should have holes arranged in a spiral.

(4) Vzorkovadlo in the closed state is inserted into the seed tube
mutually rotated so that the slots of the inner tube was opened, and gently shaken
with it in order to completely filled seeds. Then again closes
pulls and discharged into a suitable hopper or to a suitable support.
During closing should be exercised to avoid damage to the seed.
For a sub-sample is considered to be the entire contents of a type vzorkovadla,
rather than the content of one of its chambers.

2.1.3. Vzorkovadlo for high costs and bulk seed

(1) This type vzorkovadla can be applied to the seeds is less than the seed
species Triticum aestivum.

(2) consists of a separate chamber secured to the shank.
Lower part of the chamber is of conical shape with a pointed end.
To achieve greater depth may be gradually extended handle
screwing other attachments. The chamber is a closing system which can be formed either
ring on the outside of the tool, or a wing associated with the door or flap
spring loaded. Some of these can be vzorkovadla
closed before pulling out of the sampling point, the other can not conclude
so filled chamber pulled out in the open state. For all kinds
may be the minimum internal diameter of the chamber is about 35 mm and a height of 75 mm
.


(3) Vzorkovadlo in the closed state is inserted into the seed gently
vertically pressed so that the tip has reached the desired position, withdrawn
approximately 10 cm back or to rotate (by the closure system)
gently shaking it to completely filled seeds, carefully closed if it is possible
, withdrawn and emptied into a suitable container. When closing the
should be exercised to avoid damage to the seed.

2.1.4. News subsamples hands

(1) Taking subsamples hands is the best method of sampling in the case
following genera: Agropyron, Agrostis, Alopecurus,
Anthoxanthum, Arrhenatherum, Bromus, Cynodon, Cynosurus, Dactylis,
Deschampsia, Elytrigia, Festuca, Holcus, Lolium, Panicum, Poa and
Trisetum. This method is also most suitable for sampling seeds
which could be damaged by using vzorkovadel such
velkosemenné legumes, seeds or seeds with wings
low humidity, and also for seeding and seeding strip mats.

(2) For the removal of partial samples by hand from seed in containers must be
accessible to all places inside the package. For this purpose may be cases when sampling
partially or completely emptied. Packaging, in which not all layers
seed accessible during normal opening the package, it may be necessary
cut, remove them from the sample and then repack the seed.

(3) Sampling the hands is performed so that the net open hand inserted into the desired depth
seed grips with his hand and the hand is pulled out, whereby
care must be taken that the fingers around the entrapped
seed firmly clamped so that no seeds can not escape from the palm.

2.1.5. Sampling using Auto vzorkovadla

2.1.5.1. Using auto vzorkovadla

(1) sub-samples are taken from the current seed when filling into containers.

(2) Requirements for automatic operation vzorkovadla

A) sub-samples are taken from the current seed evenly across its
section and the frequency of sampling is controlled by a timer,

B) sub-samples are piped from vzorkovadla into a container collection
closet

C) seeds of this facility shall reflection or otherwise spontaneously
fall outside the collecting vessel,

D) the contents of one container must match the size of the aggregate sample,

E) collecting box must be lockable

F) vzorkovadla cabinet, timer and all connections to the leadership of seed
vzorkovadla into a collecting box shall be sealed,

G) vzorkovadlo not damage the seed or to choose between different
seeds on the basis of their size, buoyancy or pluchatosti.

(3) Automatic vzorkovadlo installed according to the manufacturer
technological conditions and its operation for the purpose of taking official samples must be approved
Institute.

(4) When using the automatic vzorkovadla for the official taking of samples
this vzorkovadlo locked and accessible only to authorized
samplers.

(5) Additional requirements for operation and security of automatic vzorkovadla

A) vzorkovadla mechanism including a timer is placed in a closed cabinet
,

B) pipeline between vzorkovadlem and collecting box is guided in such a
length of the seeds fall into the collection container could cause any damage
sampled seed

C) if it is above a collecting box pipe split and doubled,
collects a sample for laboratory cleaning station into a container outside the header box,

D) collecting box is firmly attached to the base and secured against any displacement
, spontaneous opening and must meet the following conditions
:

First in the mantle may be other openings than the opening for the supply of seed
slot for inserting the ticket indicating the batch number, pin hole outer
operating mechanism rotary device with collection containers and handling
roomy enough hole to remove the collecting containers
samples

Second inside comprises a rotatable device with the individual collecting containers.
The operation of this device shall be so constructed that it was outside the cabinet
turntable can be operated in one direction only between 360 without the possibility
reverse movement; rearward movement of the turntable, or rewind to the start position
is only possible with an open closet,

Third each container must be evenly spaced so that even when
If considerable quantities of seeds could cause deterioration of the sample

Mixing seed from more containers

Fourth keys to the cabinets have samplers, the second key is stored in a sealed envelope samplers
u
responsible worker cleaning station,

E) periods of time switch must be set so that the time required to clean
seed lots have been removed so vzorkovadlem
subsamples that its weight corresponding to approximately four times
laboratory sample.

(6) When setting a clock cycle does not match the capacity of the collection container,
can be used to sample from one batch two receptacles with
prescribed designation.

(7) The operation of automatic vzorkovadla keeping records data
identical on tickets vhazovaných into containers complemented by the kind
variety and the weight of the sampled portion. This register controls the worker
Institute.

2.1.5.2 Workflow service auto vzorkovadla

(1) Before cleaning the seed lot attendant throws slot into the collection container
ticket with a batch number, date and time of the beginning of the cleaning
lot and makes an entry into the book an automatic vzorkovadla.

(2) After cleaning the lot attendant again throws slot into the collection container
ticket with a batch number, date and time of completion of the cleaning
game and makes an entry into the book an automatic vzorkovadla.

(3) rotates the turntable one place so that the supply of seed pod was
empty vessel and tosses a ticket for another game.

(4) The procedure is repeated as many times as lots being prepared between individual
official sampling samplers, or if you just
capacity collecting box.

2.1.5.3 Workflow samplers

(1) samplers aggregate samples taken from the closet and prepares
laboratory, Reserve, and possibly other official sample, while
check conformity of data on each container labels discards data presented in
records.

(2) After you remove all the samples inserted collection container back swivel
device that adjusts to the starting position and the header box closes and locks
.

(3) If the sample in the container does not have the necessary weight to carry
whole new sampling manually.

2.1.6 Manual sampling of current seed

Sub-samples may be using hand tools to draw from the current seed when
packages, provided that the sub-samples are taken from the current
seed evenly across its cross-section tools and seeds of reflection or
otherwise they do not fall out spontaneously.

Third Number of subsamples in relation to the type and number of packages

(1) From the seed lots in bags or other containers of the same size and type
one weighing 15-100 kg sub-samples are taken in the minimum number
:


Table 1
--------------------- ------------------------
----------------------- Number of packages in the lot Number of subsamples
-------------- ------- ------------------------------------------- ----
1
5 subsamples 2-4 3 sub-samples from each container
5-8 2 sub-samples of each package
9-15 1 subsample of each package
16-30 15 sub-samples of seed lots
31-59 20 sub-samples of seed lots
60-154 30 sub-samples of seed lots
155-400 always at least one sub-sample of five packages
401-566 subsamples of 80 containers
567 or more times at least one subsample of 7 packages --------------------- ----
-------------------------------------------

For lots of containers of less than 15 kg packages are merged into sampling units
exceeding 100 kg, for example, 20 packages of 5 kg, 33
packaging of 3 kg or 100 packages of 1 kg. In the case osivových belts and osivových
mats can be bags or disk merged into sampling units
exceeding 2 million seeds. Sample units are then considered
packaging and sampling is carried out according to Table 1.

(2) Where lots in containers larger than 100 kg (including seed in bulk)
or sampling of current seeds are taken sub-samples by
lot weight in this minimum number:


Table 2
------------------------------ --------------- -------------------------------------------------- --------
Weight Number of subsamples
------------------------------ - --------------------------------------------------
--------------------- up to 500 kg, at least five subsamples

501-3 000 subsample from about one out of every 300 kg, at least 5 subsamples
3001 20 000 from about one subsample of 500 kg each, but at least 10 subsamples
20,001 and more kg sub-sample one out of every 700 kg, at least 40 subsamples
------------------------------ --- -------------------------------------------------- --------------------

When sampling a lot of the number of packages of 15 or less from each package
regardless of their size, have taken the same number of increments.
Sampling pelleted, inlay, a granular seed osivových
belts and osivových mats takes place according to Tables 1 and 2.

Fourth Sampling for the determination of warehouse pests

(1) To determine the warehouse pests with sub-samples are taken directly into
airtight container and the resulting aggregate sample is sent directly to
analysis. Samples are taken alternately

A) from the bottom of the bag (up to 200mm)

B) from a part of the bag 200 mm from the circumferential layer

C) delivery of the bag over 200 mm from the inner layer.

(2) Number of subsamples is set out in Table 1

(3) seeds in bulk containers and bulk seed is collected
sub-samples in locations where it can be assumed occurrence
warehouse pests (damp places, places with floors, walls, etc.).

(4) The sample size for detecting warehouse pests is identical
prescribed weight for laboratory sample except
oil and fiber plant for which a prescribed weight of 250 g.

Fifth Preparation of the aggregate and laboratory sample

(1) The aggregate sample is created by merging the sub-samples, where it appears as a single
. If the sub-samples does not appear as a single, sampling
the game must be stopped. If sub-samples collected directly into
one container, the container contents can be regarded as the aggregate sample
only if the seed in the container appears to be uniform. Otherwise
contents of the container can not be used to obtain a test sample.
When mixing aggregate sample repeatedly using one of
mechanical dělidel sample must be between uses
dělidla agitated manually.

(2) If the aggregate sample is difficult in terms of store mix and reduce
, delivers a whole to the laboratory to be reduced. As applicable
only in cases where the sample is not required reserve. If the aggregate size
pattern matching, is regarded as the laboratory sample without
reduction.

(3) Laboratory sample, reserve sample and another sample or to request
vendor is prepared by mixing and reduction of the aggregate sample
to the appropriate size, and one of these methods, while
in the reduction of one sample these methods can be combined:

A) Mechanical division is suitable for all kinds of seeds except
very heavily flowing species. Performed various types of mechanical
dělidel, which must meet the following requirements:

First provide at least one representative portion of the total amount
split sample

Second properly divided and heterogeneous material or mixture of seeds
species with different sizes and with different physical properties of seeds and ingredients

Third there must be changes in the composition of the material to be cut and to change the function
dělidla during division (electrostatic charge capture and
additional release of some components)

Fourth maintaining uniform operating speed of moving parts
exceeding 1 m / diameter of the ways in which material passes must be at least 2.5 times
larger than the maximum size of seeds

Fifth maintain a constant dividing ratio with a tolerance of + - 3%.

B) Method division hands - its use is limited to families Anthoxanthum,
Arrhenatherum and Trisetum. For all other species can be used only for obtaining
specimens in laboratory testing
health condition. The seed is poured evenly on a smooth, clean surface thoroughly with a spatula
mixed with straight edge and creates a cluster of him that
then divided into halves, and a total of three times, so that a part of the eight
. They are arranged in two rows of four and then mixed
all odd parts together and all even parts together thereby
resulting halves of the original quantity of seed.

C) Method division spoon - recommended for preparing the sample for testing

Health, among other tests is limited to species with seeds
smaller than wheat seeds. After preliminary mixing seed
sprinkle evenly on a tray, which is then no longer shaky, and using
spoons and spatulas are collected seeds from at least five randomly selected sites
.

D) Modified halving method - tool consists of a tray and grid
which half the cubic cells lacking the bottom. After preliminary mixing
seeds are evenly sprinkle on the grid. After lifting the grid will remain on
tray approximately half of the sample.

(4) Once a test sample or half
test sample to the rest of the laboratory sample mix again before preparing
another test sample, or half the sample.

(5) The sample preparation to test the moisture is necessary to proceed so
to minimize changes in the humidity of the sample. The seed must not be removed during reduction
sample exposed to the air outside the package for longer than 30 seconds
. Can proceed so that the seed is first mixed either in its packaging
spoon or using other similar container, which opening
attached to the container opening with a sample, and seed mix přesypáváním
from one container to another . Then seed spoon is removed from at least three different locations
and the portion of the sample are combined together.

(6) In the case osivových belts and osivových mats randomly collected
of strips or mats.

(7) When using any of these dělidel sample preparation
pelleted seed may not exceed the height of fall of 250 mm.

6th Sampling of seed potatoes

(1) Sampling to determine the health status of sampling
seed potatoes for the determination of disease infestation is carried out either directly from
seed crops, or from warehouses. From the warehouse takes samplers
samples control, inspection and possibly samples of seed lots for which
sampling has been carried out from the undergrowth.

A) sampling procedure for seed potatoes from seed crops:

First aggregate sample is obtained by combining five subsamples which
taken from five different locations staggered spaced across land and
always of two adjacent lines in each of the 11 clusters for consecutive
themselves | ||
Second from each clump is taken one or two seed tuber size.
When taking the two tubers of one tuber placed in a sample for laboratory test
a second sample to test for field growing.

B) If the sample was removed from the stand, taken at random from
stored seed tubers of average size, with one sample containing 110
tubers. The number of samples depending on the area and
degree of propagation, down Table 3.

Table 3
---------------------------- ----------------- ------------------------------------
degree of propagation Number of tubers for post- test
- --------------------------- ----------------------- ------------------------------
SE1, SE2 to 1 ha - 1 x 110 tubers
1.1 - 2 hectares - 2 x 110 tubers
2.1 - 3 hectares - 3 x 110 tubers
etc.
---------------------------- ----------------- ------------------------------------
E1 - E3, A, B Within 3 ha - 1 x 110 tubers
3.1 - 6 ha - 2 x 110 tubers
6.1 - 9 - 3 x 110 tubers
etc.
---------------------------- ----------------- ------------------------------------

C) Sampling of seed potatoes from packaging

Table 4
---------- ----------------------------------- ------------------------------------------------- | || Saving seed count method for taking incremental samples
partial
Sample
----------------------------------- ----------- ------------------------------------------------ || | bags 10 of 10 randomly selected containers alternately from upper, middle and
lower layer 3 of the bag must be completely empty.

Box pallets and 5 of 5 randomly selected containers and 2 from the top, two from Central
bulk bags and one from the lower layers of packaging, which must be emptied.

Bulk 10 of 10 evenly spaced locations alternately from the top,
middle and lower layers.
----------------------------------- ------------ -----------------------------------------------


(2) Sampling seed on mechanical analysis

A) sub-samples of seed potatoes after harvest are taken randomly handfuls
(clamping both hands), including particulate contaminants so that the sample
represent average condition seedlings

B) if the sample analysis not performed on the spot, shall be sent for analysis in
packaging, which must be clean, dry and well breathable and labeled according
model set out in Appendix 2. Containers provided by the supplier,

C) when sampling seed potatoes from packaging to proceed in accordance with Table 4.

7th Sampling jig onion and garlic

(1) From the seed lot jig onion and garlic in packages weighing over 30 kg
are taken alternately individual sub-samples from the upper, middle and lower part
in quantities required for assembly of the aggregate sample as follows
:


Table 5
--------------------- ------------------------
---------------------------------------- Number of packages in the lot Number of subsamples
--------------------- -------------------------- --------------------------------------
1-10 subsample from each package | || 11 -30 subsamples of every third package, at least from 10obalů
over 31 sub-samples of every fifth package, but at least 10 packages
-------------- ------- ------------------------------------------- ---------------------

(2) The seed lot in packages weighing 30 kg or less packaging
assembled to form a sampling unit for approximately l00 kg. Each
sampling unit is regarded as one package, wherein the procedure is according to paragraph 1.


(3) For bulk seed subsamples are taken from various depths, and
a lot of weight.

Table 6
---------------- ----------------------------- -----------
Weight lot of incremental samples
---------------------------- - ---------------------------
up to 1 ton 5 subsamples
over 1 ton of 10 subsamples
- ---------------------- ---------------------------- -----

(4) Sub-samples are taken by hand or trowel into large
vessel where it is mixed thoroughly.

(5) With planting garlic average sample compiled from sub-samples
.

(6) For jig onions from sub-samples form a bulk sample whose
mass is four times greater than the mass of the laboratory sample
set out in Part I of Annex no. 5. The aggregate sample is laid out in squares || | and divides two milliamperes diagonals into four triangles, one of which is
used as a laboratory sample.

(7) Reserve samples are prepared only at the request of the contractor.

8th Sampling vegetable seedlings

(1) Inspection of vegetable seedlings is performed by a supplier in the period when
are clearly visible growth characteristics of individual species and can
assess their health status. Samples were taken prior to shipment.

(2) The minimum sample size determined samplers
based on the total number of seedlings; controls 1% grown seedlings.

(3) Seedlings of packaging - cups are not removed individually, but taken
entire cultivation trays are staggered in sequence and number specified
samplers according to lot size.

(4) In the case of suspected generic ingredient or impaired health
is collected and assessed a sample of twice the size.

(5) The growing test sampled a specific number of seedlings
according to individual species. Samplers ensure delivery of samples to the appropriate
test station of the Institute.

(6) Plants attacked by disease or pests delivers
samplers in the laboratory of the Institute.

9th When detects heterogeneity of the seed lot

9.1 Test values ​​H

9.1.1 Definitions and symbols

Testing heterogeneity within the lot (in-range), with the aid
properties (e.g. purity, germination, HTS), taken as an indicator is based on comparison of the detected
variance (variance) and acceptable scatter || | this property. Individual samples of a seed lot samples are collected
independently from different containers. Testing of samples from
individual containers on a particular property must also be independent of each other
. Since it is available for each package only one source
information is not directly included in the examination of heterogeneity within
individual packages. Allowable variance is calculated by multiplying
theoretical variance of the random distribution of values ​​for f

Additional variation, taking into account the level of heterogeneity that
is achievable in conditions of good manufacturing practice osivářské.
Theoretical scattering can be calculated from the respective probability distribution, which is
binomial distribution in case of purity and germination, and
Poisson distribution for the number of other seeds.


In
Value H: H = ----- - f

W

H Negative values ​​are reported as zero.

F values ​​for the additional variation in seed lots for calculating the W and H values ​​
:

Table 7
-------------------------------------------- - ------------------------
Signs seeds that are difficult-flowing
not characterized by difficult seeds
Bulk
-------------------------------------------- - -----------------------

Cleanliness 1.1 1.2 Number of other seed germination 1.4 2.2 1
1 ------------------------------------------ 1.2
- -------------------------

Notes:

- For purity and germination count result to two decimal places,
If n is less than 10, and to three decimal places, if N is 10 or more
.

- For a number of seeds of other plant species count result to one decimal place
if N is smaller than 10, and to two decimal places,
if N is 10 or more.

- For difficult-flowing seeds are considered the following genera or species
:

Agropyron, Agrostis, Alopecurus, Anethum, Anthoxanthum, Anthriscus,
APIUM, Arrhenatherum, Avena, Beta, Bromus, Cichorium, Cynodon,
Cynosurus, Dactylis, Dacus, Deschampsia, Elytrigia, Fagopyrum,
Festuca, xFestulolium, Foeniculum, Holcus, Lactuca, Lolium,
Lycopersicon, Onobrychis, Panicum, Pastinaca, Petroselinum, Phacelia,
Phalaris, Phleum, Pimpinella, Poa, Rheum, Scorzonera, Setaria, Sorghum, Spinacia
, Trisetum, Triticum spelta, Triticum dicoccon, Valerianella.

9.1.2 Sampling from the game

Number of independent subsamples of individual containers must not be less than
shown in Table 8.

Coatings from which to sample, are selected strictly at random.
Sample taken from the packaging needs to adequately represent all of its contents, such
. upper, middle and lower part. The weight of each subsample
not be less than half the minimum weight of the laboratory sample
fixed for a given species in Annex no. 5 of this Decree.

Sampling intensity and the critical values ​​of H. The number of independent sub
samples to be taken depending on the number of packages in the lot, and
critical value H heterogeneity in seed lots at a significance level of 1%
probability:

Table 8
--------------------- ------------------------
------------------------- Number Number critical value critical value H H
independent packaging for signs of purity and character for the number of seeds
game partial germination of other plants
Sample kinds
from individual
packages --------------------------------------------
good hard good hard
-flowing-flowing-flowing-flowing
seeds seeds seeds seeds
------------------------ ------------------- ---------------------------
5 5 2.55 2.78 3.25 5.10
6 6 2.22 2.42 2.83 4.44
7 7 1.98 2.17 2.52 3.98
8 8 1.80 1.97 2.30 3.61
9 9 1.66 1.81 2.11 3.32
10 10 1.55 1.69 1.97 3.10
11-15 11 1.45 1.58 1.85 2.90
16-25 15 1.19 1.31 1.51 2.40
26-35 17 1.10 1.20 1.40 2.20
36-49 18 1.07 1.16 1.36 2.13
50 or more 20 0.99 1.09 1.26 2.00 ------------------------ -------
---------------------------------------

9.1.3 Test Procedure

Properties Evaluation to detect heterogeneity may be:

A) weight percentages of any folder purity

B) the percentage of any component testing of germination

C) the total number of seeds of other plant species or the number of seeds
any single species in the determination of seeds of other plant species
.

In the laboratory of each sub-sample from the individual wrapper removes
one sample and subjected to the test for the selected property

Independently of any other sample.

Ad a) you can use the weight percentages of any component for
provided that it is possible to separate the analysis of purity, e.g. pure
seeds, seeds of other plant species, or grass seeds deaf.
The sample should be of such weight that according to preliminary estimates
contained 1000 seeds, calculated from each subsample. Each sample
is divided into two fractions: the selected component and the rest.

Ad b) You can use any kind of seed or key ring, which can be
establish a standard germination test, for example. Keychain normal, abnormal
keychain or hard seeds. For each sub-sample is simultaneously deployed
one test germination rate of 100 seeds.

Ad c) You can use any number of folders that can be counted, for example.
Number of seeds of a certain type or number of seeds of other plant species
together. Each sample shall be estimated so that
contained approximately 10,000 seeds, and calculates in it the selected folder.

9.1.4 Using Tables 8 and reporting of results

Table 8 shows the critical value H, corresponding to 1% of the test seed lots
with the permissible distribution of assessed property.
When the calculated value of H exceeds a critical value H, which includes a sample number N
appropriate properties and Bulk in Table 8, then given the game
considered as one that shows statistically significant heterogeneity within the meaning
in-range (within a file), or possibly also off-range
(off set). If, however, the calculated value of H less than or equal
critical value H in the table, then the given batch regard as
which exhibits heterogeneity in terms of in-range (within a file), or where appropriate, of
off-range (outside file) as regards properties,
which is being tested.

H test results values ​​are interpreted as follows:
_
X, N, No. calculated value of H and the statement that "The value of H
is a proof / evidence is not statistically significant heterogeneity."

_
If X is outside the limits below, the value of H is not calculated or
stated in the report:

component detected during test purity: more than 99.8% or less than 0.2%

germination: more than 99.0% or less than 1.0%

certain number of seeds: less than two sample

9.2 Test values ​​R

The test aims to detect the presence of heterogeneity of the seed lot
off-range (except file) using features, taken as an indicator.
Test of heterogeneity off-range (except file) includes comparing
biggest difference found between the samples of similar size
taken from the game with the tolerated range of values. This tolerated
range is based on the permissible standard deviation that is
achievable in conditions of good manufacturing practice osivářské.

Each independent sub-sample is taken from another container, so
heterogeneity within the packaging is not directly included in the study. Information
heterogeneity within the containers is nevertheless included in the permissible standard deviation
which is actually embodied in the tables tolerance ranges.
Permissible standard deviation was calculated from the standard deviation
influenced by random distribution, according to the binomial distribution in case
purity and germination, and according to the Poisson distribution for the number
seeds of other plant species, multiplied by the square root values f
listed in table 7. the mutual variability between the containers is characterized
calculated range, to be compared with corresponding
tolerated range.

9.2.1 Definitions and symbols

Well, number of packages in the lot
N number of independent sub-samples of individual containers
n number of seeds tested from each individual sample (1,000
purity, germination and 100 to 10,000 for the number of other seeds | || plant species)
X value of the test features in a single sample from the bag
sum total of all values ​​


X _ average of the sum of all the values ​​observed X
X = ----- properties set for that game
N

R = Xmax - Xmin range determined as the difference between the maximum
independent sub-samples from each
packaging parts for endpoint


Note:

- For purity and germination count result to two decimal places,
If n is less than 10, and to three decimal places, if N is 10 or more
.


- For a number of seeds of other plant species count result to one decimal place
if N is smaller than 10, and to two decimal places,
if N is 10 or more.

9.2.2 Sampling from the game

Sampling to test the value of R is the same as for test values ​​H;
Need to use the same samples.

9.2.3 Test Procedure

To test the value of R used the same testing procedures purity
germination of seeds and other plant species, such as are used for test
value H. Calculations must use the same set of data.

9.2.4 Use tables and reporting of results

Off-range heterogeneity of the seed lot is tested by the appropriate tables with
tolerated, ie. The critical range:

Table 9 for component cleanliness examination detected

Table 10 for germination testing, and

Table 11 for the number of seeds of other plant species.

Finds the value of X in the column labeled "Average" appropriate table.
Average value is rounded off in conventional manner.
Reads the tolerated range that would be exceeded only 1% of the test seed lots with an acceptable division
character:

In column 5-9 for the cases where N = 5-9

Column 10 to 19 for the cases where N = 10 to 19, or

Column 20 for the cases where N = 20.

If the calculated value of R exceeds the tolerable range, then given
lot deems game showing a statistically significant heterogeneity within the meaning
off-range. If the calculated R-value is less than or equal
tolerated range contained in the tables, then the game is deemed
game showing no heterogeneity in terms of off-range, if
respect to property that is being tested.

Test Results R-values ​​are interpreted as follows:

X, N, No, the calculated value of R and a statement that "This value is R
proof / evidence is not statistically significant heterogeneity."

When using tables with averages rounded to the next level table
value, if the diameter of exactly midway between the two
tabulated values, rounded down.
9.3 Interpretation of results


Whenever that either of the two tests, test values ​​of H or R
test values, demonstrating the presence of significant heterogeneity
must be given batch declared heterogeneous. But when neither of the two
tests proved statistically significant heterogeneity must be given
game considered a game that is heterogeneous, meaning the game,
which is statistically insignificant levels of heterogeneity.

To point 9.2.4 Maximum tolerated ranges to test the value of R
at the level of significance of 1% probability using ingredients analysis purity
as assessed property in good sypatelných seeds:


Table 9 Part 1.
--------- ---------------------------------
---------------------------- Average Average tolerance range, the tolerance range for
percentages of percentages for the number of representations
independent sample representation of independent samples
given folder (N) and the component (N) and its
of

----------------- Accessories Accessories ---------------- ---------------------------------- ---
5-9 10-19 20 5-9 10-19 20
------------------- ---------------------
99.9 0.1 0.5 0.5 0.6 88.0 12.0 5.0 5.6 6.1
99.8 0.2 0.7 0.8 0.8 87.0 13.0 5.1 5.8 6.3
99.7 0.3 0.8 0.9 1.0 86.0 14.0 5.3 5.9 6.5
99.6 0.4 1.0 1.1 1.2 85.0 15.0 5.4 6.1 6.7
99.5 0.5 1.1 1.2 1.3 84.0 16.0 5.6 6.3 6.9
99.4 0.6 1.2 1.3 1.4 83.0 17.0 5.7 6.4 7.0
99.3 0.7 1.3 1.4 1.6 82.0 18.0 5.9 6.6 7.2
99.2 0.8 1.4 1.5 1.7 81.0 19.0 6.0 6.7 7.4
99.1 0.9 1.4 1.6 1.8 80.0 20.0 6.1 6.8 7.5
99.0 1.0 1.5 1.7 1.9 78.0 22.0 6.3 7.1 7.8
98.5 1.5 1.9 2.1 2.3 76.0 24.0 6.5 7.3 8.0
98.0 2.0 2.1 2.4 2.6 74.0 26.0 6.7 7.5 8.2
97.5 2.5 2.4 2.7 2.9 72.0 28.0 6.9 7.7 8.4
97.0 3.0 2.6 2.9 3.2 70.0 30.0 7.0 7.8 8.6
96.5 3.5 2.8 3.1 3.4 68.0 32.0 7.1 8.0 8.7
96.0 4.0 3.0 3.4 3.7 66.0 34.0 7.2 8.1 8.9
95.5 4.5 3.2 3.5 3.9 64.0 36.0 7.3 8.2 9.0
95.0 5.0 3.3 3.7 4.1 62.0 38.0 7.4 8.3 9.1
94.0 6.0 3.6 4.1 4.5 60.0 40.0 7.5 8.4 9.2
93.0 7.0 3.9 4.4 4.8 58.0 42.0 7.5 8.4 9.2
92.0 8.0 4.1 4.6 5.1 56.0 44.0 7.6 8.5 9.3

91.0 9.0 4.4 4.9 5.4 54.0 46.0 7.6 8.5 9.3
90.0 10.0 4.6 5.1 5.6 52.0 48.0 7.6 8.6 9.4
89.0 11.0 4.8 5.4 5.9 50.0 50.0 7.6 8.6 9.4 -----------------
---------------- ---------------------------------- ---



To point 9.2.4

Maximum tolerated ranges to test the value of R
significance level of 1% probability for use of constituents as purity analysis
rated properties in difficult sypatelných seeds:

Table 9 Part 2
--------- ---------------------------------
---------------------------- Average Average tolerance range, the tolerance range for
percentages of percentages for the number of representations
independent sample representation of independent samples
given folder (N) and the component (N)

her and her accessories accessories
------------------- ---------------------
5-9 10-19 20 5-9 10-19 20
--------------------------------- -------------------------------------
99.9 0.1 0.5 0.6 0.6 88.0 12.0 5.2 5.8 6.4
99.8 0.2 0.7 0.8 0.9 87.0 13.0 5.4 6.0 6.6
99.7 0.3 0.9 1.0 1.1 86.0 14.0 5.5 6.2 6.8
99.6 0.4 1.0 1.1 1.2 85.0 15.0 5.7 6.4 7.0
99.5 0.5 1.1 1.3 1.4 84.0 16.0 5.8 6.6 7.2
99.4 0.6 1.2 1.4 1.5 83.0 17.0 6.0 6.7 7.4
99.3 0.7 1.3 1.5 1.6 82.0 18.0 6.1 6.9 7.5
99.2 0.8 1.4 1.6 1.7 81.0 19.0 6.3 7.0 7.7
99.1 0.9 1.5 1.7 1.8 80.0 20.0 6.4 7.1 7.8
99.0 1.0 1.6 1.8 1.9 78.0 22.0 6.6 7.4 8.1
98.5 1.5 1.9 2.2 2.4 76.0 24.0 6.8 7.6 8.4
98.0 2.0 2.2 2.5 2.7 74.0 26.0 7.0 7.8 8.6
97.5 2.5 2.5 2.8 3.1 72.0 28.0 7.2 8.0 8.8
97.0 3.0 2.7 3.0 3.3 70.0 30.0 7.3 8.2 9.0
96.5 3.5 2.9 3.3 3.6 68.0 32.0 7.4 8.3 9.1
96.0 4.0 3.1 3.5 3.8 66.0 34.0 7.5 8.5 9.3
95.5 4.5 3.3 3.7 4.1 64.0 36.0 7.6 8.6 9.4
95.0 5.0 3.5 3.9 4.3 62.0 38.0 7.7 8.7 9.5
94.0 6.0 3.8 4.2 4.6 60.0 40.0 7.8 8.8 9.6
93.0 7.0 4.1 4.6 5.0 58.0 42.0 7.9 8.8 9.7
92.0 8.0 4.3 4.8 5.3 56.0 44.0 7.9 8.9 9.7
91.0 9.0 4.6 5.1 5.6 54.0 46.0 7.9 8.9 9.8
90.0 10.0 4.8 5.4 5.9 52.0 48.0 8.0 8.9 9.8
89.0 11.0 5.0 5.6 6.1 50.0 50.0 8.0 8.9 9.8 -----------------
---------------- ---------------------------------- ---





To point 9.2.4

Maximum tolerated ranges to test the value of R
significance level of 1% probability for use of constituents as germination tests
assessed property in good sypatelných seeds:

Table 10 Part 1.
--------- ---------------------------------
---------------------------- Average Average tolerance range, the tolerance range for
percentages of percentages for the number of representations
independent sample representation of independent samples
given folder (N) and the component (N)

her and her accessories accessories
------------------- ---------------------
5-9 10-19 20 5-9 10-19 20
--------------------------------- -------------------------------------
99 1 5 6 6 74 26 22 24 26
98 2 7 8 9 73 27 22 25 27
97 3 9 10 11 72 28 22 25 27
96 4 10 11 12 71 29 22 25 27
95 5 11 12 13 70 30 23 25 28
94 6 12 13 15 69 31 23 26 28
93 7 13 14 16 68 32 23 26 28
92 8 14 15 17 67 33 23 26 28
91 9 14 16 17 66 34 23 26 29
90 10 15 17 18 65 35 24 26 29
89 11 16 17 19 64 36 24 26 29
88 12 16 18 20 63 37 24 27 29
87 13 17 19 20 62 38 24 27 29
86 14 17 19 21 61 39 24 27 29
85 15 18 20 22 60 40 24 27 30
84 16 18 20 22 59 41 24 27 30
83 17 19 21 23 58 42 24 27 30
82 18 19 21 23 57 43 24 27 30
81 19 19 22 24 56 44 24 27 30
80 20 20 22 24 55 45 25 27 30
79 21 20 23 25 54 46 25 27 30
78 22 20 23 25 53 47 25 28 30
77 23 21 23 25 52 48 25 28 30
76 24 21 24 26 51 49 25 28 30
75 25 21 24 26 50 50 25 28 30 ---- ---------------------------------
---------------------------------


To point 9.2.4


Maximum tolerated ranges to test the value of R
significance level of 1% probability for use of constituents as germination tests
rated properties in difficult sypatelných seeds:
Table 10 Part 2
--------- ---------------------------------
---------------------------- Average Average tolerance range, the tolerance range for
percentages of percentages for the number of representations
independent sample representation of independent samples
given folder (N) and the component (N)

her and her accessories accessories
------------------- ---------------------
5-9 10-19 20 5-9 10-19 20
--------------------------------- -------------------------------------
99 1 6 6 7 74 26 23 25 28
98 2 8 8 9 73 27 23 26 28
97 3 9 10 11 72 28 23 26 28
96 4 10 12 13 71 29 23 26 29
95 5 11 13 14 70 30 24 26 29
94 6 12 14 15 69 31 24 27 29
93 7 13 15 16 68 32 24 27 29
92 8 14 16 17 67 33 24 27 30
91 9 15 17 18 66 34 24 27 30
90 10 16 17 19 65 35 25 27 30
89 11 16 18 20 64 36 25 28 30
88 12 17 19 21 63 37 25 28 30
87 13 17 20 21 62 38 25 28 31
86 14 18 20 22 61 39 25 28 31
85 15 18 21 23 60 40 25 28 31
84 16 19 21 23 59 41 25 28 31
83 17 19 22 24 58 42 25 28 31
82 18 20 22 24 57 43 25 28 31
81 19 20 23 25 56 44 26 29 31
80 20 21 23 25 55 45 26 29 31
79 21 21 24 26 54 46 26 29 31
78 22 21 24 26 53 47 26 29 31
77 23 22 24 27 52 48 26 29 31
76 24 22 25 27 51 49 26 29 31
75 25 22 25 27 50 50 26 29 31 ---- ---------------------------------
---------------------------------



To point 9.2.4

Maximum tolerated ranges to test the value of R
significance level of 1% probability for use of seeds of other plant species
as assessed property in good sypatelných seeds:
Table 11 Part 1.
-------- ----------------------------------
-------------------------------- Average Average tolerated tolerated tolerated
average number range for the number range NY number range for the number of seeds seeds
number of seeds of different independent
another independent of another independent
plant samples (N) of plant samples (N) of plant samples (N)
kind kind kind

-------------- -------------- -------------------
5-9 10-19 20 5-9 10-19 20 5-9 10-19 20
-------------------------- ---------------------------------------- -------- || | 1 6 7 7 47 38 42 46 93 53 59 65
2 8 9 10 48 38 43 47 94 53 60 65
3 10 11 12 49 39 43 47 95 54 60 66
4 11 13 14 50 39 44 48 96 54 60 66
5 13 14 15 51 39 44 48 97 54 61 66
6 14 15 17 52 40 45 49 98 54 61 67
7 15 17 18 53 40 45 49 99
55 61 67 8 16 18 19 54 40 45 50 100 55 62 67
9 17 19 21 55 41 46 50 101 55 62 68
10 18 2 22 56 41 46 51 102 55 62 68 || | 11 19 21 23 57 42 47 51 103 56 62 68
12 19 22 24 58 42 47 51 104 56 63 69
13 20 23 25 59 42 47 52 105 56 63 69
14 2 23 26 60 43 48 52 106 57 63 69
15 22 24 26 61 43 48 53 107 57 64 70
16 22 25 27 62 43 49 53 108 57 64 70
17 23 26 28 63 44 49 54 109
57 64 70 18 24 26 29 64 44 49 54 110 58 65 71
19 24 27 30 65 44 50 54 111 58 65 71
20 25 28 30 66 45 50 55 112 58 65 71 || | 21 25 28 31 67 45 50 55 113 58 65 72
22 26 29 32 68 45 51 56 114 59 66 72
23 27 30 33 69 46 51 56 115 59 66 72
24 27 30 33 70 46 52 56 116 59 66 73
25 28 31 34 71 46 52 57 117 59 67 73
26 28 32 3 72 47 52 57 118 60 67 73
27 29 32 35 73 47 53 58 119
60 67 73 28 29 33 36 74 47 53 58 120 60 67 74
29 30 33 37 75 48 53 58 121 60 68 74
30 30 34 37 76 48 54 59 122 61 68 74 || | 31 31 34 38 77 48 54 59 123 61 68 75

32 31 35 38 78 49 54 60 124 61 68 75
33 32 36 39 79 49 55 60 125 61 69 75
34 32 36 39 80 49 55 60 126 62 69 76 35 33
37 40 81 49 55 61 127 62 69 76
36 33 37 41 82 50 56 61 128 62 70 76
37 34 38 41 83 50 56 61 129 62 70 76
38 34 38 42 84 50 56 62 130 63 70 77
39 34 39 42 85 51 57 62 131 63 70 77
40 35 39 43 86 51 57 62 132 63 71 77
41 35 40 43 87 51 57 63 133 63 71 78
42 36 40 44 88 52 58 63 134 64 71 78
43 36 41 44 89 52 58 64 135 64 71 78
44 37 41 45 90 52 58 64 136 64 72 78 45 37
41 45 91 52 59 64 137 64 72 79
46 37 42 46 92 53 59 65 138 64 72 79 ----------------------
-------------------------------------- ------------ -

To point 9.2.4

Maximum tolerated ranges to test the value of R
significance level of 1% probability for use of seeds of other plant species
as assessed property for difficult sypatelných seeds:

Table 11 Part 2
-------- ----------------------------------
tolerated -------------------------------- Average Average Average tolerated tolerated
number range for the number range for number range for the number of seeds seeds
number of seeds of different independent
another independent of another independent
plant samples (N) of plant samples (N) of plant samples (N)
kind kind kind
-------------- --------------- -----------------
5-9 10-19 20 5-9 10-19 20 5-9 10-19 20
-------------------------- ---------------------------------------- -------- || | 1 7 8 9 47 47 53 58 93 66 74 81
2 10 11 12 48 48 54 59 94 67 75 82
3 12 14 15 49 48 54 59 95 67 75 82
4 14 16 17 50 49 55 60 96 67 75 83
5 16 18 19 51 49 55 60 97 68 76 83
6 17 19 21 52 50 56 61 98 68 76 83
7 19 21 23 53 50 56 62 99
68 77 84 8 20 22 24 54 51 57 62 100 69 77 84
9 21 23 26 55 51 57 63 101 69 77 85
10 22 25 27 56 52 58 63 102 69 78 85 || | 11 23 26 28 57 52 58 64 103 70 78 86
12 24 27 30 58 52 59 64 104 70 79 86
13 25 28 31 59 53 59 65 105 70 79 86
14 26 29 32 60 53 60 65 106 71 79 87
15 27 30 33 61 54 60 66 107 71 80 87
16 28 31 34 62 54 61 66 108 71 80 88
17 29 32 35 63 55 61 67 109
72 80 88 18 29 33 36 64 55 62 68 110 72 81 88
19 30 34 37 65 56 62 68 111 72 81 89
20 31 35 38 66 56 63 69 112 73 81 89 || | 21 32 36 39 67 56 63 69 113 73 82 90
22 33 36 40 68 57 64 70 114 73 82 90
23 33 37 41 69 57 64 70 115 74 83 90
24 34 38 42 70 58 65 71 116 74 83 91
25 35 39 42 71 58 65 71 117 74 83 91
26 35 40 43 72 58 65 72 118 75 84 92
27 36 40 44 73 59 66 72 119
75 84 92 28 37 41 45 74 59 66 73 120 75 84 92
29 37 42 46 75 60 67 73 121 76 85 93
30 38 42 46 76 60 67 74 122 76 85 93 || | 31 38 43 47 77 60 68 74 123 76 85 93
32 39 44 48 78 61 68 75 124 76 86 94
33 40 44 49 79 61 69 75 125 77 86 94
34 40 45 49 80 62 69 75 126 77 86 95
35 41 46 50 81 62 69 76 127 77 87 95
36 41 46 51 82 62 70 76 128 78 87 95
37 42 47 51 83 63 70 77 129
78 87 96 38 43 48 52 84 63 71 77 130 78 88 96
39 43 48 53 85 63 71 78 131 79 88 96
40 44 49 54 86 64 71 78 132 79 88 97 || | 41 44 50 54 87 64 72 79 133 79 89 97
42 45 50 55 88 65 72 79 134 79 89 98
43 45 51 55 89 65 73 80 135 80 89 98
44 46 51 56 90 65 73 80 136 80 90 98
45 46 52 57 91 66 74 80 137 80 90 99
46 47 52 57 92 66 74 81 138 81 90 99 ---------
------------------------- ------------------------- ---------------
Appendix 2


Sample forms and documents

(1) Sample forms

A) application for control of seed

B) requests for sampling

C) a request for electrophoresis

D) printing sample

E) forms for keeping records in the production of planting material
(discounted label), the form can also be made in electronic form

F) forms for keeping records of the labels at the supplier, the form is

Can also be made in electronic form

G) the forms protocol on disposal of labels

H) the format of the label sample (application) to determine the health status of seed potatoes
(ELISA) or for mechanical analysis of seed potatoes

I) a sample of the labels on the vegetation test

J) labels sample of seed potatoes to other countries of origin

(2) document model

A) Protocol on assessment tests vegetation



Appendix Protocol vegetation assessment test crops



Appendix Protocol vegetation assessment tests vegetables


Appendix 3


Procedure for evaluating seed crops

First Policies

(1) seed crop is assessed at the time when the plant development
most suitable for assessment of their crucial properties. Each
the crop has been overlooked at least once. Number of shows
is determined for each species.

(2) When the show crop at present deputy
supplier.

(3) In the assessment of the seed crops shall be checked

A) compliance with the sequence of crops - the supplier is obliged to state in the application for recognition
stand what crop in the reference period on the land
grown. Forecrop sequence control is performed

First when submitting the application,

Second before performing the show crop shall be in written records
breeder (books, eventually. furlongs card)

Third at own show crop species

B) compliance with the prescribed minimum distances to avoid

First adulteration during the growing season or during the harvest from neighboring
stand (hereinafter referred to as "mechanical isolation"). Failure mechanical insulation
will be treated as so. Correctable fault. After its removal may
contractor ask for a show of control,

Second possible transmission of pollen for cross-pollinated species or disease transmission
(hereinafter "spatial isolation"). Spatial isolation is checked out
submitted cadastral maps and in the field to show propagation
stand. Failure spatial isolation is assessed as unrecoverable
fault. Vegetation in this case does not recognize and will not be overlooked. For
large seed crops, it is possible to create sufficient space
department isolation of the crop species and then enable control
show,

C) the crop at the show's format, in which the assessed

First the overall condition of vegetation

Second purity kind

Third purity variety

Fourth weed,

Fifth the occurrence of harmful organisms.

(4) Not if their growth parameters values ​​categories for which
application was made for recognition, but matches the rating category lower
vegetation can be recognized in a lower category on the basis of new applications supplier with || | indication of the equivalent category and generation. The assessments are subject to
newly introduced category.

(5) The Contractor may submit a request to halt the proceedings for recognition
crop at (hereinafter "Out of the crop species").
Out for the crops of the same principles apply:

A) check-out is possible only before the execution of the show, at the workplace
Institute, which filed a request

B) at the time of its ongoing exhibitions supplier shall report this fact
simultaneously přehližiteli,

C) unless the crop is logged off and does not meet the conditions for
version of the show, to which přehližitel arrived too will be exposed
"The decision on non-recognition of the crop species", the costs associated with this
administrative performance of suppliers will be charged.

(6) If you suspect that changing the state of the crop species
after removing the crop at fault or in case of disagreement
supplier with the result of the show of the crop species it is possible to control
show. The procedures are as follows:

A) throughout the vegetation, especially when reasonable suspicion that
condition of the crop at the time of the show has changed, can přehližitel
perform inspection parade and change the original assessment. About this
shall immediately inform the contractor

B) the Contractor may request the inspection show only stands
unrecognized for removable defects. After troubleshooting requests in writing
on inspection parade of the Institute for which application was submitted
crop certification and assuming that the crop is

Still in the growing phase, which is suitable for carrying out control
show.

Removable defects are

First generic and varietal ingredients

Second weed,

Third failure of mechanical insulation

Fourth failure to comply with the spatial isolation, if it is possible to rectify the fault
additional mechanical interference according to par. 3 point. b) Section 2

C) If the supplier does not agree with the outcome of the show crop species
may be in writing or in electronic form to ask for a commission
control-show at the Department of seeds and seedlings in Prague.
Control show will be performed with contributions
Institute, representatives of the contractor and possibly invited experts. Convening
ensure all participants Department. The show will be performed under the following assumptions
:

First will stand still in the growing phase suitable for evaluation,

Second will present přehližitel who performed the previous show
vegetation

Third in the stand were carried out interventions that would affect the state
vegetation

(7) For the types or categories for which it is prescribed more
shows seed crops, in particular shows
evaluate the properties of the seed crops established for that show. Every
parade will prepare a record.

(8) Evaluation of seed crops by certification schemes
Organization for Economic Cooperation and Development coincides with
their assessment under national legislation. An integral part
certification under these schemes is to perform input and output
vegetation test in Annex # 4.

Second Rating crop at a parade

(1) Přehližitel makes the crop at the show and on the basis
observation fills the record of the outcome of the show crop species
whose specimen is given in Annex no. 2 and decide on final evaluation, which also recorded
to form.

(2) passes Přehližitel stand to obtain a comprehensive picture of the overall state
. The growths of up to 1 ha passes across
surface larger growths according to the shape of the land along the diagonals (Fig. 1)
optionally in the direction of the longer axis according to Fig. 2.



(3) During the show makes přehližitel at random locations
vegetation assessment. One partial evaluation is always carried out on an area of ​​100 m2
while walking direction change. The number of partial evaluation is based on the acreage of land



Tab. 1
--------------------------------- ------------- ---------------
Acreage propagation minimum number
crop assessment
------------ --------------------------------- ----------------
1-20 ha 5
over 20 - 50 h and 10
More than 50 ha 15
--------- --------------------------------- -------------------


(4) In the case of potatoes and other row crops (eg. Jigs) the occurrence of diseases and impurities
evaluated 100 consecutive plants and the number
evaluation is based on the following table:

Tab. 2
--------------------------------- ------------- ---------------
Acreage propagation minimum number
crop assessment
------------ --------------------------------- ----------------
1-3 ha 5
Over 3 - 20 h and 10
More than 20 ha for every 3 ha 1 rating
---- --------------------------------- ------------------------


(5) In sub-trials will proceed as follows:

A) in case of detected limits the number of reviews doubles

B) vegetable impurities is calculated every whole fertile plants not
individual offshoots,

C) arithmetic mean calculated at partial character evaluation is the sum of the values ​​of character
divided by the number of ratings.

With the exception of characters, the limit is set as a number with decimal places
, the result is rounded up to integers. Numbers less than or equal to 0.5
are rounded to 0, numbers greater than 0.5 are rounded up to 1

(6) For the final evaluation and registration in the "Record of the outcome of the crop at the show
" used simultaneously expressing
spot and verbal. Use downward point scale
labeled 9, 7, 5, 3 and 1


Tab. 3
-------------- -------------------------------- -----------------
Spot Description *) **) ***)
ratings

-------------- --------------------------------- ----------------
9 very good condition, with no gaps; u
countable fault relates to the rarity
incidence to 0.5 (inclusive) or outside the counting;
verbal expression is generally not used

7 good condition, minimal gaps; u
countable faults - reaching a maximum of 50%
set values ​​

5 state average, shortcomings still permitted in
limit value; for countable faults -
the occurrence of a maximum in the border set
values ​​

3 mediocre condition, gaps are larger
extent than is provided, but additional
intervention are removable; countable faults
They are beyond the specified values;
growth will not be recognized after the deficiencies
You can ask for a show of control

1 very bad condition, gaps are large
range and can not remove it is a
growth that is in very poor condition entirely
flattened, heavily weedy; selection
generic or varietal admixture does not seem
in regard; vegetation shall not be recognized; if the supplier
Results show disagrees, he may request
Control-a commission show
-------------- -----------------------------
-------------------- *) where growth evaluated with one of the characteristics 1 point
Other properties are already not assess
**) if at any of the parameters prescribed by decree "must
occur, "growth will not be recognized by the detection of a single
pest or plant
***) if at any of the parameters prescribed by decree "null"
occurrence, it is possible to tolerate sporadic occurrence (up to a diameter of 0.5
included) or counting off (accidentally found, a unique
fault).

(7) The record of the outcome of the crop at the show

A) přehližitel receives from the Department of preprinted basic information about
growers, the location of the crop species, origin of the seed from which the crop
based, category and generation and preceding crop acreage.
The form consists of original and 3 copies of color. The original is stored at the Institute
first copy (yellow) for the Contractor, the second copy (pink)
grower receives during the parade, the third copy (blue) remains přehližiteli,

B) complete, sign and stamp marks přehližitel and representatives
contractor or grower. Thus completed and signed forms
are the basis for the issue of recognition of lading or decision on non-recognition
crop at a further proof for the supplier to perform
show. In the absence of supplier or his representative
(grower) at the show in the form under the heading "result show
took note" shall note: "supplier (grower) away"
copy of the record is then sent to suppliers mail.
This procedure can not be used in case no vegetation

C) in the case includes non-recognition and non-recognition of reason, which is indicated in the heading
"comment".

Third Technological progress in the evaluation of the crop at

3.1 overall state of vegetation

(1) Rating is based on the assessment of the overall condition of the plants found
throughout the show, not only in the partial counting not only on seed
evaluation and assessment of species and varietal purity, but a complete view of
aspect of vegetation balance, engagement
polehlosti, weed, overall health, and even in relation to
yield and quality of the expected harvest.

(2) Scoring
Tab. 4
-------------- -------------------------------- -----------------
Spot Description
-------------------------------- -------------- evaluation
-----------------
9 stand perfectly connected, vegetation
balanced, nepolehlý, an area not healthy, with
prerequisite for high yield
(Among other indicators are not used
lower scores than 7)

7 stand well connected, flattened max. 25
% Surface vegetation not quite balanced, with
which can weed and health status
assess the word lightly, assuming good
revenue

5 sparse vegetation, polehlost over 25% of the area
erratic, weed and health status
Central, assuming the average yield

3 spotty vegetation, sparse, strongly flattened (above

50%, when it can be reliably assessed
generic and varietal purity) strongly
weedy, in poor health, with
prerequisite substandard yield

1 stand practically is invalid, the degree
polehlosti does not allow assessment of the authenticity and
purity varieties, weedy strongly, strongly
damaged pests and disease, the assumption
Harvest is minimal
-------------- ----------------------------- --------------------

(3) to evaluate the overall condition of the plants include the estimated total harvest,
přehližitel introduces him at the last prescribed show (or show
control) crop, is cited estimate of the total harvest of clean
overlooked area.

03.02 Cleanliness kind

(1) Value impurities other plant species of the same group crops
possibly related species,

A) which is a maximum incidence in undergone field
number or a percentage,

B) whose presence is not undergone field set. These kinds
will result record to show the crop shall be recorded
descending order, by the strength of coverage at a lower occurrence will be recorded and manpower


(2) For the identified impurities is given full generic name, or the name of an ancestral
with an indication of the number.

(3) Scoring
Tab. 5
-------------- -------------------------------- -----------------
Spot Description
-------------------------------- -------------- evaluation
-----------------
And 9) the crop is completely free of impurities different kind
(Max. Occurrence - rare, plant species
beyond counting)
b) the crop without the occurrence or minimal
the occurrence of other species

7) the occurrence of other plant species reach
a maximum of 50% of the specified values ​​
b) the occurrence of weak

And 5) the occurrence of other plant species reach
maximum specified values ​​
b) The mean incidence of other plant species
it being possible to evaluate the purity and identity
varieties

And 3) the occurrence of plants of other species exceeds
specified values, in quantities which can
still make the selection and allow control
show

b) strong prevalence of other plant species
downstream of seeds of these species from seed
cleaning irremovable, the authenticity and purity
variety is difficult to evaluate

1) the occurrence of plants of other plant species in
quantity which has not selecting
b) the occurrence of plant very strong, it is not possible
evaluate other characteristics needed
the recognition of vegetation
------------------------------ -------------- -------------------

(4) Other plant species according to section 3.2. paragraph. 1 letter a) and b) to
result record crop at the show recorded once
point value, and it is always the lower of the two detected.

3.3 Cleanliness variety

(1) evaluated the incidence of other varieties and distinctly divergent types u
kind consideration. Evaluation is carried out according to the official description
morphology of plants of that variety.

(2) Verbal Appraisals - přehližitel indicate the number of individual variations.
The total sum of the deviations will be listed numerically on record in the appropriate box on the right
. U stands in the SE and E přehližitel shall moreover
brief description of diverging plants expressing variance from the description
variety.

(3) Scoring
Tab. 6
-------------- -------------------------------- -----------------
Spot Description
-------------------------------- -------------- evaluation
-----------------
9 vegetation is completely free of impurities and varietal
distinctly different types (maximum occurrence -
sporadically, counting off)

7 the occurrence of plants of other varieties and clearly
diverging type achieves a maximum of 50%
set values ​​

5 occurrence of plants of other varieties and clearly
divergent types reaches maximum specified
values ​​

3 occurrence of plants of other varieties and clearly
of off-types exceeds the specified value, and
in an amount which can still perform selection and
allow inspection parade

1 occurrence of plants of other varieties and clearly

of off-types in the quantity which has already
the selection does not, or due to confusion
variety.
-------------- --------------------------------- ----------------


3.4 weed infestation
(1) evaluated the incidence of other plant species, weed and cultural
not subject to evaluation in 3.2. These are the kinds

A) which is a maximum incidence in undergone field
number or a percentage,

B) whose presence is not undergone field set. These kinds
will result record to show the crop shall be recorded
descending order, depending on the strength of coverage.

(2) For the identified impurities other plant species that are
limited in number, the ancestral states and the generic name for other
impurity states at least the title race.

(3) Scoring
Tab. 7
-------------- -------------------------------- -----------------
Spot Description
-------------------------------- -------------- evaluation
-----------------
And 9) the crop without the occurrence of other specified
plant species
b) the crop without the occurrence or minimal
the occurrence of other plant species

7) the crop with the occurrence of a maximum of 50% of the number
provided other plant species
b) weak weed

And 5) the crop with the appearance of other vegetable
species not exceeding a specified value
b) The mean incidence of other vegetable
species, whereby it is possible to evaluate the purity and
varietal authenticity

And 3) the occurrence of other plant species
excess of the specified values, while
selection in growth is possible
b) strong prevalence of other plant species
downstream of seeds of these species
from seed cleaning indelible, authenticity and
purity of the variety is difficult to evaluate

1) the occurrence of plants of other plant species
in an amount of several times in excess
set values ​​does not select for
determining the occurrence can be used estimate
b) very strong weed infestation, and can not be
evaluate other characteristics necessary to recognize
-------------------------------- -------------- stand
-----------------

(4) Other plant species according to section 3.4. paragraph. 1 letter a) and b) to
result record crop at the show recorded once
point value, and it is always the lower of the two detected.

3.5 Health status

(1) evaluated the occurrence of harmful organisms (pests and diseases) in
undergone field. These are harmful organisms

A) which is a maximum incidence in undergone field
number or a percentage,

B) whose presence is not undergone field set, but it is entered into
result record crop at and taken into account when
severe crop damage.

(2) In the event of quarantine harmful organisms to stand
recognized.

(3) Scoring


Tab. 8
-------------- -------------------------------- -----------------
Spot Description
-------------------------------- -------------- evaluation
-----------------
And 9) the crop free of plants
infected monitored harmful organisms
or the occurrence of sporadic
b) rare occurrence of harmful organisms
in undergone field

7) the crop infested with the appearance
Plant more than 50% of a fixed number
b) weak presence of harmful organisms
in undergone field

5 b) the crop infested with the appearance
plants not exceeding a specified value
c) secondary occurrence of harmful organisms
in undergone field

And 3) the crop infested with the appearance
Plant above a specified value, the selection
in the stand can be done, if appropriate
b) the strong presence of harmful organisms
in undergone field

1 very strong presence of harmful organisms
the occurrence of quarantine harmful organisms
-------------- ----------------------------- --------------------

(4) Harmful organisms according to paragraph 3.5. paragraph 1, letter a) and b) to
result record crop at the show recorded once

Point value, and it is always the lower of the two detected.
Appendix 4


The rules for establishing and assessing vegetation testing

First Purpose and Principle vegetation tests

(1) Vegetation test allows the assessment of properties
propagating material in characters that are difficult to ascertain other methods,
or other methods of determining the evidence is low or too costly
.

(2) Vegetation test is checked generic and varietal purity and authenticity
, the percentage of hybridity, or medical condition.
It also monitors whether the process of seed multiplication no changes in the character
varieties.

(3) The identity of the variety is assessed by visual comparison
plants emerged in the control plots sown representative sample
assessed in the game and emerged plants on a plot sown standard
sample of a given variety. This determines whether the plant under consideration
sample conforms to the official description of the variety.

(4) The level of purity of the variety is assessed by counting the number of such plants emerged
control plots that do not accurately describe varieties.
This determines whether the tested sample meets set standards for cleanliness
varieties.

Second The types of vegetation tests

2.1 Vegetation testing schemes by the Organization for Economic Cooperation and Development


(1) The Institute of certified seed by schemes
Organization for Economic Cooperation and Development for the varietal certification of seed moving on
international market. The certificates, which confirm the identity and purity of seed
are issued assuming satisfactory result
entrance exams vegetation, sampling output growing
exam and satisfactory result field shows or shows.

A) entrance exam vegetation:

First exam takes place in the same or previous, growing season,
which is evaluated by the crop,

Second guest entrance exams vegetative propagation
input material may be used in the assessment of the crop species

Third vegetation assessment tests should coincide with the evaluation
crop species

Fourth in case of any defects to determine their causes and assess the severity
. If this is not outside influences, these deficiencies
reason for not issuing a certificate

B) output growing test:

First exam takes place in the growing season, when the game is given
already recognized and put into circulation, possibly planted on the surface of conventional breeding,
therefore, in case of any defects can be done by any measure
only those parts or parts that have not been put into circulation

Second compared with plants grown from seeds certified lot with
plants grown from the standard sample, so the results
growing output test is used to check whether during the multiplication of seeds
no adverse changes in the varietal purity

Third Output vegetation test may be simultaneously input vegetation
test for seed the next generation

Fourth Output vegetation test is necessary based on hybrid varieties
because in these cases it can not assess the authenticity and purity of varieties in seed
undergone field.

2.2 Field vegetation examination as a follow-up and control of seed
put into circulation

Is done in the following cases:

A) breeding propagating material which is marketed,

B) planting material precursors and essential
propagating material of such varieties, which in previous recognition procedure
seed crops appeared to be problematic or where the supplier
first lodged an application for recognition

C) in controlling the production and marketing of propagating material into circulation

D) in control standard seed and seedlings of vegetables,

E) the control of selected seed and seedlings imported from third countries
,

F) the control of authorized persons

G) the control of officially unregistered varieties

H) complaints supplies seed

I) seed lots, where there is doubt as to the authenticity or purity
variety

J) following up hybridity,

A) when controlling seed of conservation varieties

L) when controlling seed varieties bred for growing under particular
conditions

M) in control auto vzorkovadla.

Checking 2.3 percent hybridity


The test is done by means of subsequent control of hybrid seed varieties.
Method of performing tests for individual species are listed in Table 1. Table
.
1
----------------------------------------------- -----------------------------------
sorts of processes and evaluation methods
---- -------------------------------------------------- ----------------------------
oilseed rape crop is based in duplicate at different locations;
hybridism is checked at the stage of flowering plants, the
is evaluated, each plant individually.
Parental components are controlled similarly, not only
one location.
----------------------------------------------- -----------------------------------
rye checked restorer and technical mixture. Consideration should be given
to the content of 5-8% in the technical restorer composition. If žese
not the varieties grown in technical mixtures, taken into account when
establishment and evaluation of vegetation testing methodology to the breeder.
----------------------------------------------- -----------------------------------
wheat while setting and vegetation assessment tests are taken into account
the methodology breeder.
----------------------------------------------- -----------------------------------
tomato seed is sown cups, assessment is carried out in phase
the first true leaf. Assesses the true leaf shape.
----------------------------------------------- -----------------------------------
pepper seeds are sown cups, seedlings are placed into the greenhouse ;
evaluates the individual plants with fully developed fruit. according
variety description to evaluate plant, shape, position and color
fetus.
----------------------------------------------- -----------------------------------
cucumber plants are evaluated individually. According to the description of the variety is assessed
plant, type of flowering and fruit.
----------------------------------------------- -----------------------------------
kale, cabbage detects the occurrence of parental components.
----------------------------------------------- -----------------------------------
other identifies the incidence and prevalence of parental components
non-hybrid plants.
----------------------------------------------- -----------------------------------

2.4 Subsequent inspection of seed potatoes

Shall be checked seedlings in recognition procedures in the category SE1 (possibly
SE2), new varieties included in state varietal tests if requested
recognition procedure, the varieties having the test result by ELISA
higher than the average result of the health status of all varieties will be
year inspection samples and seed imported and exported parts.

2.5 percent Determining the occurrence of weed beets in seed, sugar and fodder beet


In determining the incidence of weed seeds in seed beet sugar and fodder beet is growing
test being formed in duplicate with a total area of ​​45
m ^ 2, where it is grown without singling about 2,000 plants.

Third Technological progress

(1) During the growing test determines whether the plants grown on
control plots from the test sample corresponding to their properties

A) the official description of the variety

B) plants standard sample,

C) the values ​​set for the examination of seed crops.

(2) For most plant species are growing tests are based on
selected varietal characteristics listed in the guidelines of UPOV (Union for the
Protection of New Varieties of Plants) for distinctness, uniformity and stability
, under which they were registered varieties.

3.1 Test samples

The minimum sample weights for vegetation tests are given in Annex
no. 5. The final delivery dates vegetation samples for testing to the test
stations are listed in Table 2. The sample, which is supplied to the test
station after the deadline specified in Table 2, it will not be included in the
growing season to test ; sample may be included in the tests
following growing season.

Table. 2

----------------------------------------------- ---------------------------

Type term --------------- --------------------------------------------------
--------- --------------------------------- Field crops
-----------------------------------------

Cereals - spring 15.3.
- Corn 15.04

- Winter barley 09.15
- Other winter cereals 30.9.
----------------------------------------------- ---------------------------

Legumes - winter (incl. Pannonian vetch and hairy vetch) 15.9.
- Spring 15.3.
----------------------------------------------- ---------------------------
Oilseeds spring 15/03
Oilseeds winter 20/08
- Rape exam hybridity 08.15
----------------------------------------------- ---------------------------
Legumes 30.3.
- Purple clover 08.20
----------------------------------------------- ---------------------------

grasses - ryegrass-year 31.3.
- Italian ryegrass 31.8.
- Perennial grasses 15.4.
----------------------------------------------- ---------------------------
bluebell 15.3.
----------------------------------------------- ---------------------------
turnip 31.1.
----------------------------------------------- ---------------------------
diabetes, diabetes - assessment of weed beets 15.3.
----------------------------------------------- ---------------------------
fodder beet 15.3.
----------------------------------------------- ---------------------------
potato - planting 15.3.
----------------------------------------------- --------------------------- ----------------

VEGETABLES -------------------------------------------------- --------
spring onions and garlic, chicory, peas bone marrow, 31.1.
Medium early or very late varieties of cabbage, cauliflower and
cabbage, carrots, red pepper, chives, parsley,
leeks, tomato, radish, beet salad, lettuce
summer and autumn, Chinese cabbage, turnips
----------------------------------------------- ---------------------------
beans, corn, melons, watermelons, squash 28.2.
----------------------------------------------- ---------------------------
cucumber field 31.3.
----------------------------------------------- ---------------------------
onion winter 30/07
----------------------------------------------- ---------------------------
garlic winter 09/30
----------------------------------------------- ---------------------------
broccoli, celery, kale, very early until 31.12
semi-early varieties of cabbage, cauliflower and cabbage ,
kohlrabi, cucumber forcing, radishes, lettuce and
for forcing spring, spinach
-------------------------- ------------------------------------------------ || |

3.2 Standard sample

(1) The standard sample is a sample of seed varieties with which the test sample
compared in order to verify the authenticity and varietal purity.
Is always sown in the vicinity of the test sample.

(2) The standard sample is used

A) sample supplied by the breeder at the Institute of Registration variety

B) a sample Institute may request from the breeder or the maintainer
variety

C) the variety registered abroad sample supplied at the request of the Institute
official authority of the country.

(3) The standard sample can be used

A) sample taken for official seed planting materials and precursors
basic propagating material

B) certified planting material first generation of synthetic
varieties allogamních and all kinds of hybrid varieties

C) parental inbred lines and components in the categories of reproductive material
precursors or basic propagating materials which are
used to produce hybrid varieties.

These samples may be used, provided that for seeds was compared
uniformity and stability with the sample supplied by the Institute breeder when
registration variety.

(4) In case of loss of germination or consuming seed stock the Institute
require a new standard sample, in advance
least one growing season.

3.3 plots for growing test

(1) Vegetation tests based on Institute testing stations in
field conditions maloparcelních tests or in protected areas
greenhouses or greenhouses.

(2) Vegetation exam is based in warmer climate areas with irrigation
option for faster germination, which guarantees the possibility of an earlier evaluation
plants than in the crop vegetation.


(3) The land on which the plots with vegetation tests placed,
not be swamped with wild plants of the same or closely related species
a succession of crops must ensure that the seed of the land inventory
affect ratings test.

(4) The test species are grown on control plots in accordance with
agrotechnical principles for the plant species.

(5) In the control plots restricting the use of herbicides, fertilizers and growth regulators
order not to affect the morphology of plants and prevent lodging
stand.

(6) Test plots are distributed on the land by plant species and varieties
vegetation type test according to the following criteria:

A) the test plot is located close to the standard plot
sample the variety

B) samples of related parts of the same variety and similar varieties are sown
side by side

C) if the sample is sown in multiple variations, repetitions must be placed
to other parts of the land, especially in species where the evaluation carried out
measurements.

(7) Minimum acreage control plots, or the lowest number
plants for each species are listed in Table 3. Table
.
3
----------------------------------------------- -----------------------------------------
Type Category Area m2 *)
----------------------------------------------- -----------------------------------------
cereals, maize, pulses, oilseeds, SE, E 30
legumes, fodder crops, grasses C 10
(Except Kentucky bluegrass and perennial)
---------------------------------------- ------------------------------------------------ || | meadow grass SE, E 20
C 10
--------------------------------------------- -------------------------------------------
ryegrass SE, E 50
C 10
--------------------------------------------- -------------------------------------------
sugar beet, fodder beet
- vegetation exam E, C 20
- weed beet C 45
---------------------------- -------------------------------------------------- ----------
potato - planting SE, E, A, B 100 tubers
---------------------- -------------------------------------------------- ----------------
broccoli, celery, bean climbing, SE, E, C, S 60 plants
kale, cabbage, kale, cauliflower,
corn (population), cucumber field,
chives, lettuces, cabbages, Chinese and Beijing

---------------------- -------------------------------------------------- ----------------
onions, carrots, parsnips, SE, E, C, S 200 plants
parsley (root and Nate), leeks, radishes, || | beetroot, spinach
------------------------------------------ ----------------------------------------------
chicory , garlic, peas, E, C, S 100 plants
---------------------------------- -------------------------------------------------- ----
Salsify SE, E, C, S 300 plants
----------------------------- -------------------------------------------------- ---------
bean (Dwarf) SE, E, C, S 150 plants
--------------------- -------------------------------------------------- -----------------
corn (hybrid) E, C 40 plants
----------------- -------------------------------------------------- ---------------------
pepper and tomato (the field) SE, E, C, S 40 plants
------- -------------------------------------------------- -------------------------------
sugar melon, water melon, SE, E, C, S 20 plants | || forcing cucumber, pepper and tomato (in rapid growth)
radish, pumpkin
---------------------------- -------------------------------------------------- ----------
tomato (hybridity) C 200 plants
--------------------------- -------------------------------------------------- -----------
turnips SE, E, C, S 80 plants
----------------------- -------------------------------------------------- ---------------
vegetable seedlings (by species) SE, E, C, S from 20 to 100 plants
----------- -------------------------------------------------- --------------------------- *
) if crop requires assessments are instead given the number of plants needed for evaluation.

Fourth Test procedure for evaluation of vegetation

Ongoing evaluation purity can be carried out throughout the period

Plant growth and development, which are obvious signs of that variety.
Varietal characters are divided into primary and secondary. When assessing the need to focus primarily on
different and unusual plants. These detailed description and
visible marks that have not been evaluated repeatedly.
An estimate of the number of individuals per plot and calculate the differing types.
Determined values ​​are compared with tables. If the number of aberrant plants or equal
exceeds the value, evaluation is repeated.

4.1 Evaluation of authenticity and purity of agricultural species except
corn in vegetative trials

Rating purity is carried out for each species according
table # 4.

Tab. no. 4 Method for assessing purity of individual species.

------------------------------------------------------------------------------------------------------
Type SE, EC1 C2
------------------------------------------------------------------------------------------------------
Legumes and clovers
------------------------------------------------------------------------------------------------------
Peas, pea, bean 99.7% 99.0% 98.0% 99.5%
Soybean 99.0% 99.0%
All other legumes and clovers, except peas,
pea, bean and soybean 1 to 10 square meters per 30m21
hybrid varieties: peas, pea, bean 99.7% 99.0% 98.0%
------------------------------------------------------------------------------------------------------
Fodder crops and oil seeds
------------------------------------------------------------------------------------------------------
Rape and turnip - besides strictly types of feed 99.9% 99.7% 99.7%
Rape and turnip - strictly feed types 99.7% 99.0% 98.0%
kale, turnip, white mustard, sunflower 99.7% 99.0% 98.0
Len% 99.7% 98.0% 97.5% 99.0
poppy% 98.0% 98.0%
Other species (same type and other variety) 1 to 10 square meters per 30m21
Other species (other species) 1 to 30m21 to 10 m2
------------------------------------------------------------------------------------------------------
Parents to hybrids
------------------------------------------------------------------------------------------------------
Řepice - varietal purity 99.9%
Řepice - sterility 98.0%
Rape - sterility - winter form 99.0%
Rape - sterility - spring form 98.0%
Line with pollen and self-pollination lines 99.9%
------------------------------------------------------------------------------------------------------
Seed lines of hybrid varieties (certified)
------------------------------------------------------------------------------------------------------
Line - varietal purity 99.0%
Line - sterility 98.0%
Řepice - line with pollen 99.5%
Rape - line with pollen 99.7%
Line of self-pollination 99.5%
Vegetation test hybrids 90.0%
------------------------------------------------------------------------------------------------------
CEREALS
------------------------------------------------------------------------------------------------------
Wheat, barley, oats 99.9% 99.7% 99.0%
Mainly self-pollination varieties of triticale 99.7% 99.0% 98.0%
Pollination hybrid varieties of rye and triticale 1 to 30 square meters to 10 square meters 1
------------------------------------------------------------------------------------------------------
Hybrid varieties:
------------------------------------------------------------------------------------------------------
Wheat, barley, oats - Parents 99.9%
wheat, barley, oats - hybrids 99.7%
Vegetation test hybrids 90.0%
------------------------------------------------------------------------------------------------------
GRASS
------------------------------------------------------------------------------------------------------
Kentucky bluegrass (except for apomictic varieties jednoklonových) 1 to 20 square meters to 10 square meters 4
Kentucky bluegrass (jednoklonové apomictic varieties) 1 to 20 square meters to 10 square meters 6
All kinds of smooth, except for Kentucky bluegrass 1 to 30 square meters to 10 square meters 1 | || All species, except for perennial 1 to 30 square meters to 10 square meters 1

Only ryegrass 1 to 50 square meters to 10 square meters 1
------------------------------------------------------------------------------------------------------

4.2 Evaluation of varietal identity and purity in maize

Rating varietal purity in maize tables shall no. 8 -
10th Ratings vary depending on the variety.
Tab. no. 8 - Breakpoints purity - headed by two-hybrid at a standard purity of 97%
------------------------------------------------------------------------------------------------------
Sample size - the number of plants Recognised Unrecognised
------------------------------------------------------------------------------------------------------

100 3 4 67-99 2 3
33-66 1 2
less than 33 0 1
------------------------------------------------------------------------------------------------------

Tab. no. 9 - Breakpoints purity - tříliniový hybrid standarduodrůdové at a purity of 95%
------------------------------------------------------------------------------------------------------
Sample size - the number of plants Recognised Unrecognised
------------------------------------------------------------------------------------------------------

6 100 5 80-99 4 5
60-79 3 4
40-59 2 3
20-39 1 2
less than 20 0 1
------------------------------------------------------------------------------------------------------

Tab. no. 10 - Breakpoints purity - when standard varietal čistoty99,0%, 99.5%, 99.9%
------------------------------------------------------------------------------------------------------
Sample size Basic seed Basic seed Certified seed
- The number of plant lines of open-pollinated variety of open-pollinated variety
99.9% 99.5% 99.0%
------------------------------------------------------------------------------------------------------
unrecognized recognized recognized recognized unrecognized unrecognized
------------------------------------------------------------------------------------------------------
Less than 1000 0 1 - -
200 0 1 1 2 2 3
100 0 1 0 1 1 2
75 0 1 0 1 0 1
50 0 1 0 1 0 1
------------------------------------------------------------------------------------------------------

Rating 4.3 percent hybridity control.

Hybridity Percentage is calculated as the ratio of the number not satisfying
plants to the total number of plants assessed. When assessing
male sterile hybrid component, in addition to determining the level of hybridity
determines whether a given component plants produce viable pollen.

4.4 Rating subsequent control of seed potatoes.

The test planted 100 bulbs (4 x 25 pieces) on the test plot.
Vegetation during each test plot checked twice,
a commission show. Vegetation examination is evaluated according to the requirements
on seed crops.

4.5 Rating subsequent inspection of standard vegetable seed.

According to the type of the value determined by the number of plants or plant amounts to
stand on the surface of an area given in Table 3

U carrots will be evaluated in a different color roots with beetroot
eyeballs with white circles and eyeballs with bold bright circles. For brood vegetables they are usually
retain the selected berries to full maturity to control
characteristics.

4.6 Evaluation of control seedlings of vegetables.

Test and its evaluation is similar to section 4.5.

4.7 Rating determine the percentage of occurrence of weed beets in seed
sugar and fodder beet.

(1) The evaluation carried out by staff of the Institute in the presence of a commission
suppliers. For each flowering plants are checked these characters weed beet
:

A) vegetative earliness,

B) the presence of anthocyanins per stem,

C) the number of flowers,

D) the number of vascular bundles at the base,

E) the strength of the root.

Fifth Keeping records of vegetation tests

(1) The result of the growing trials Department issues a report, which the model is
given in Appendix 2.


(2) For agricultural species that meet the requirements for evaluation
varietal identity and purity, suppliers are informed of the results of tests
vegetation excerpt from an electronic database of the Institute. In other cases, the issue
Department report. ". Annex 5



maximum lot weight, minimum size of laboratory samples
test samples and test specimens to the vegetation
|| |
Table 1
agricultural species and vegetables

------------------------------- - ------------------------------------------------ - --------------------------------------
Type - Czech Type - Latin Maximum minimum minimum minimum minimum
weight weight weight weight weight
lot **) test laboratory test sample to sample
kg sample to sample vegetation analysis
g g
purity test g / pc
g
--------------------------------- ------------- ------------------------------------- ------------- -------------------------
1 2 3 4 5 6 7
------------ --------------------- ----------------------------- --------------------- ----------------------------- ---------
fragrant Anise Pimpinella anisum 10 000 70 7 70 50
Cynara Artichoke carduncidus 10 000 900 90 900 50
Ber Italian (čumíza, Mohar) Setaria italica 10000 90 9 90,150
Bob field Vicia faba 30 0001000 1000 1000 2000
Bob garden Vicia faba 30 0001000 1000 1000 2000
Timothy Phleum nodosum tuberous *** 10 000 50 1 10 75
Timothy *** Phleum Prato 10 000 50 1 10 75
Broccoli Brassica oleracea 10 000 100 10 100 20

celeriac Celery Apium graveolens 10 000 25 1 10 5
Onions Allium cepa 10 000 80 8 80 70
mash onion Allium fistulosum 10 000 50 5 50 70
Chickpea Cicer arietinum 30 0,001,000 1000 1000 1000
Diabetes Beta vulgaris 20 000 500 50 500 200

leaved chicory Witloof Cichorium intybus 10 000 50 5 50 25
industrial chicory Cichorium intybus 10 000 50 5 50 100
Salsify Scorzonera hispanica 10 000 300 30 300 30
Garlic, Allium sativum 10 000 20 2 20 20
Čičorka varied Securigera varia 10 000 100 10 100 100
sorghum sorghum bicolor 30 000 900 90 900 200
sorghum sorghum x Sudan grass sorghum bicolor x S. 30 000 300 30 300 200
sudanense
Sorghum Sudan grass Sorghum sudanense 10 000 250 25 250 200
lentil Lens culinaris 30 000 600 60 600 500
Čtyřboč (New Zealand spinach) Tetragonia tetragonioides 20 200 0001000 1000 150
Echalion Allium cepa 10 000 80 8 80 70
leaved endive Cichorium endivia 10 000 40 4 40 10 Eskariol


Dwarf bean bean Phaseolus vulgaris climbing 30 0001000 700 1000 500
scarlet Runner bean Phaseolus coccineus 30 0001000 1000 1000 500
Fennel Foeniculum vulgare 10 000 180 18 180 70
*** X Festulolium Festulolium 10 000 200 6 60 200
white mustard Sinapis alba 10 000 400 20 200 250
black mustard Brassica nigra 10 000 100 4 40 250
mustard Brassica juncea 10 000 100 4 40 250
field peas (including field pea), Pisum sativum 30 900 0001000 1000 1000
peas, sugar peas

Wrinkled pea Round Pisum sativum 30 0001000 900 1000 1000
Chilli Capsicum annuum 10 000 150 15 150 10
Asparagus Asparagus officinalis 0001000 20 100 1000 100
Barley Hordeum vulgare 30 120 0001000 1000 1000
Eastern Galega Galega orientalis 10000 250 20200200

Alexandrian clover Trifolium alexandrinum 10 000 400 6 60 300/150 *
clover Trifolium pratense 10 000 300 5 50 300/150 *
purple clover Trifolium incarnatum 10 000 500 8 80 300/150 * || | White clover Trifolium repens 10 000 200 2 20 200/100 *
middle Clover Trifolium medium 10 000 300 5 50 300/150 *
Clover perverse (Persian) Trifolium resupinatum 10 000 200 2 20 300/150 * | || Clover perverse (Swedish) Trifolium hybridum 10 000 200 2 20 300/150 *
hybrid ryegrass Lolium x boucheaneum *** 10 000 200 6 60 350/200 *
ryegrass Lolium multiflorum *** 10000 200 6 60 350/200 *
ryegrass Lolium perenne *** 10 000 200 6 60 350/200 *
Kale Brassica oleracea 10 000 100 10 100 20
Savoy cabbage Brassica oleracea 10,000,100 10,100 20
Kapusta forage Brassica oleracea 10 000 200 10 100 250
Brussels sprouts Brassica oleracea 10 000 100 10 100 20
Cardoon Cynara cardunculus 10 000 900 90 900 50
Kohlrabi Brassica oleracea 10,000,100 10,100 20
Chervil Anthriscus cerefolium 10 000 60 6 60 20
Caraway Carum carvi 10 000 200 8 80 200
white sweet clover Melilotus albus 10 000 50 5 50 300/150 *
Hemp Cannabis sativa 10000 600 60600500
Dill Anethum graveolens 10 000 40 4 40 40
sulla (fetus) Hedysarum coronarium 10 0001000 30300 1000
sulla (seed) Hedysarum coronarium 10 000 400 12 120 400
Coriander Opium Coriandrum sativum 10 000 400 40 400 40
red fescue Festuca rubra *** 10 000 100 3 30 200
Fescue Festuca trachyphylla drsnolistá *** 10 000 100 5 30 200
tall fescue Festuca *** pRATENSIS 10 000 100 5 50 200
sheep's fescue Festuca ovina *** 10 000 100 2.5 30 200
tall fescue Festuca arundinacea *** 10 000 100 5 50 200
Fescue Festuca capillaceous *** filiformis 10 000 100 2.5 30 200
Kozlíček Valerianella's lettuce locusta 10 000 70 7 70 25
Maize (except sugar and popcorn) Zea mays 40 900 0001000 1000 1000

Sweet corn popcorn Zea mays 20 0001000 900 1000 1000
(40000)
Cauliflower Brassica oleracea 10 000 100 10 100 20
Len Linum usitatissimum 10 000 300 15 150 150
canary Phalaris canariensis 10 000 400 20 200 200
smaller canary Phalaris minor 10000 200 20200200
reed canary grass Phalaris arundinacea 10 000 30 3 30 200
canary Phalaris aquatica water *** 10 000 100 4 50 200
Eggplants provide Solanum melongena 10 000 150 15 150 10 Lipnice
Marsh *** Poa palustris 10,000 50 0.5 5,150
Poa nemoralis *** Poa nemoralis 10,000 50 0.5 5,150
Kentucky bluegrass *** Poapratensis 10 000 50 1 5 150 || | Rough bluegrass Poa trivialis *** 10 000 50 1 5 150
annual bluegrass Poa annua *** 10 000 50 1 10 150
Lipnice squashed Poa compressa 10,000 25 0.5 5,150
Camelina sativa Camelina sativa 10 000 40 4 40 100
white Lupine Lupinus albus 30 0001000 450 1000 1000
Blue lupine Lupinus angustifolius 30 0001000 450 1000 1000
yellow lupine Lupinus luteus 30 000 1000 450 1000 1000
marjoram Origanum majorana 10 000 25 0.5 5 0.5
poppy Papaver somniferum 10 000 50 1 10 100
Mangold Beta vulgaris 20 000 500 50 500 300
Velvet Grass Holcus lanatus 10 000 25 1 10 75 | || Melon Cucumis melo 10000150 70-100 pieces
Watermelon Citrullus lanatus 20 250 0001000 1000 100 pcs

Metlice tufted Deschampsia cespitosa 10 000 25 1 10 150

Carrot Fodder carrot Daucus carota 10 000 30 3 30 80

Gherkin Cucumber Cucumis sativus 10000150 70-400 pcs | || Oats Avena strigosa hřebílkatý 30 0001000 50 1000 1000
naked Oats Avena nuda 30 0001000 120 1000 1000
Oat Avena sativa 30 0001000 120 1000 1000
Ovsík raised *** Arrhenatherum elatius 10000200 8 80 75
Paprika Capsicum annuum 10 000 150 15 150 10
parsnip Pastinaca sativa 10 000 100 10 100 10
Chives Allium schoenoprasum 10 000 30 3 30 20
Parsley Petroselinum crispum 10 000 40 4 40 60
Fenugreek Trigonella foenum-graecum 10 000 500 45 450 300/150 '
Cynosurus cristatus crested dog's 10 000 25 2 20 150
buckwheat Fagopyrum esculentum 10 000 600 60 600 500
Leek Allium porrum 10 000 70 7 70 80
millet Panicum miliaceum 10 000 150 15 150 500 ***
meadow foxtail Alopecurus pratensis 10 000 100 3 30 150
creeping dog *** Agrostis canina 10,000 50 0 25 5150
creeping thin *** Agrostis capillaris 10 000 50 0.25 5 150
Creeping great Agrostis gigantea *** 10 000 50 0.25 5 150
creeping bentgrass Agrostis stolonifera *** 10 000 50 0.25 5 150
Wheat Triticum aestivum 30 0001000 120 1000 1000
Spelt Triticum spelta 30 0001000 270 1000 1000
durum wheat, Triticum durum 30 0001000 120 1000 1000
feathered couch grass Agropyron cristatum 10 000 40 4 40 200
couch grass middle Efytrigia intermedia 10 000 150 15 150 150
tomato Solanum Lycopersicum 10,000 20 7-5
Rhubarb Rheum rhaponticum 10 000 450 45 450 135
(Rheum rhabarbarum)
Radish rape Raphamis sativus 10 000 300 30 300 250
var. oleiformis
Radish Raphanus sativus 10 000 300 30 300 50

Radish Fodder beet Beta vulgaris 20 000 500 50 500 200
Beetroot Beta vulgaris 20 000 500 50 500 300
Řepice Brassica rapa 10 000 200 7 70 250
Rape Brassica napus 10 000 200 10 100 250
Cress Lepidium sativum 10 000 60 6 60 10
Lettuce Lactuca sativa 10 000 30 3 30 15
přeslenitý Mallow Malva verticillata 10 000 50 5 50 5
sunflower Helianthus annuus 25 200 0001000 1000 250
Soybean Glycine max 30 500 0001000 1000 1000
cocksfoot oriole Dactylis polygama 10 000 100 3 30 200 ***
Dactylis glomerata Dactylis glomerata 10 000 100 3 30 200
phacelia Phacelia tanacetifolia 10 000 300 5 50 100
defenseless brome Bromus inermis 10 000 90 9 90 75
mountain brome Bromus marginatus 10 000 200 20 200 200
brome samužníkovitý * ** Bromus catharticus 10 000 200 20 200 200
brome Bromus sitchensis sitecký *** 10 000 200 20 200 200
Safflower Carthamus tinctorius 25 000 900 90 900 100
shallot Allium cepa 10 000 80 8 80 70
Spinach Spinacia oleracea 10 000 250 25 250 150
birdsfoot-year Lotus ornithopodioides 10 000 30 3 30 300/150 *
Trefoil Lotus corniculatus 10 000 200 3 30 300/150 *
Tolic dětelová Medicago lupulina 10 000 300 5 50 300/150 *
Tomka scented Anthoxanthum odoratum 10 000 25 2 20 150
Triticale Triticosecale x 30 0001000 120 1000 1000
Trisetum flavescens Trisetum yellowish *** 10 000 50 0, 5 5 50
bermudagrass Cynodon dactylon Rings *** 10 000 50 1 10 50

Turnip Brassica napus var. 10 000 200 10 100 10
napobrassica
fíkolistá Gourd Cucurbita Ficifolia 10 000 350 180 350 100 pieces
Gourd Cucurbita pepo general 20 700 0001000 1000 100 pieces
Gourd Cucurbita maxima 20 700 0001000 1000 100 pieces
Anthyllis vulneraria Anthyllis vulneraria 10000 60 6 60 300/150 *
Sainfoin - Onobrychis fetus viciifolia 10 000 600 60 600 300/150 *
Sainfoin - Onobrychis seed viciifolia 10 000 400 40 400 300/150 *
hairy vetch Vicia villosa 30 0001000 100 1000 500
Pannonian Vetch Vicia pannonica 30 0001000 120 1000 500
vetch Vicia sativa 30 140 0001000 1000 500
Turnips Brassica rapa 10 000 70 7 70 50
variable Alfalfa Medicago x varia 10000300 10 50 300/150 *
Cress Alfalfa Medicago sativa 10 000 300 5 50 300/150 *
white cabbage Brassica oleracea 10 000 100 10 100 20
red cabbage Brassica oleracea 10 000 100 10 100 20
Chinese cabbage Brassica rapa 10 000 70 7 70 20
Chinese cabbage Brassica rapa 10 000 70 7 70 50
Rye Secale cereale 30 0001000 120 1000 1000 ----------
----------------------- --------------------------- ----------------------- --------------------------- ----------- *
) sample weight for input / output growing test **
) in brackets are the weight according to ISTA rules, if they are different from the masses under EU || | ***) the maximum weight of a lot may be increased to 25,000 kg when dodavatelobdržel permission from the Department. "

Maximum lot weight, the minimum weight or size
laboratory specimens and tests on growing seedlings


Table 2 --------- --------------------------------- ----------------------------------------- --------- ------
Type in Latin kind of seed Maximum Minimum weight The minimum weight
lot weight and sample size for
laboratorníhovegetační test
sample
Kg Kg pieces or pieces
--------- --------------------------------- ----------------------------------------- --------- ------
Solanum tuberosum Seed potatoes 40 000 25 kg 110 tubers
- Mechanical analysis

Allium cepa onion seedlings, 5000 80 1 kg onion
shallots

Allium sativum Garlic 2500100 onion onions
120 --------------------------------- - ------------------------------------------------ -
-------------
The minimum sample sizes for pelleted, encrusted and granulated seed


Table 3 -------------------------- ------- ------------------------------------------- -------
Assay type Laboratory test sample
sample at least
at least (number of units)
(Number of units) --------------------------------- ----------
----------------------------------------
purity testing (including testing the authenticity 7 500 2500
kind)

Mass determination of thousands of seeds 7,500 of net
pellets

Testing germination 7,500,400

Computation of seeds of other plant species, 10,000 and 7,500
pathogenic departments

Computation of seeds of other plant species 25,000 25,000
and pathogenic forms (
encrusted and granulated seed)

Sized 10,000 2,000 --------------------------------- -----
---------------------------------------------

The minimum sample sizes for seeding bands and mats Table 4

------------------------------- - ------------------------------------------------ -
Assay type Laboratory test sample
sample at least
at least (number of seeds)
(Number of seeds) --------------------------------- ----------
----------------------------------------
Testing kind of authenticity 2500100 | || Testing germination 2500400

Purity testing (if required) 2000 2500
Computation of seeds of other plant species 10,000 7,500

departments and pathogenic -------------- ------------------- ------------------------------- -------------------

Maximum permitted lot size for granular and pelleted seed
seeding bands and mats:

Maximum number of seeds, which may include batch or pelleted
granular seed osivových strips or mats is 1000000000 (10
000 units to 100 000 units), except that the weight of such parts || | including coating material must not exceed 40,000 kg with a tolerance of 5%
(42,000 kg).

The maximum allowable weight of a lot for the inlay and treated seed:

The values ​​given in Table 1 apply to seed weight without wrapper produces
substances.

1) Council Directive 66/401 / EEC of 14 June 1966 on the marketing of seed
fodder market.

Council Directive 66/402 / EEC of 14 June 1966 on the marketing of seed
cereal market.

Council Directive 2002/54 / EC of 13 June 2002 on the marketing of beet seed
market.

Council Directive 2002/55 / ​​EC of 13 June 2002 on the marketing of vegetable seed
market.

Council Directive 2002/56 / EC of 13 June 2002 on the marketing of seed potatoes
market.

Council Directive 2002/57 / EC of 13 June 2002 on the marketing of seed of oil and fiber plants
market.

Council Directive 2008/72 / EC of 15 July 2008 on the marketing of seed and planting material
than seed.

Council Directive 2003/61 / EC of 18 June 2003 amending
Directive 66/401 / EEC on the marketing of fodder plant seed, 66/402 / EEC on the marketing
cereal seed 68/193 / EEC on the vine
vegetative propagation material on the market, 92/33 / EEC on the marketing of propagating and planting material
vegetables except seed, 92/34 / EEC on the marketing of propagating
material of fruit plants and fruit plants intended for
fruit production, 98/56 / EC on the marketing of propagating material
ornamental plants, 2002/54 / EC on the marketing of beet seed,
2002 / 55 / EC on the marketing of vegetable seed, 2002/56 / EC on the marketing of seed potatoes on the market
and 2002/57 / EC on the marketing of seed oil and fiber
products on the market with regard to comparative tests and trials .

Commission Directive 2006/124 / EC of 5 December 2006 amending
Council Directive 92/33 / EEC on the marketing of seed and planting material
of vegetable seed and Council Directive 2002/55 / EC on the marketing of vegetable seed
market.

Commission Directive 2009/74 / EC of 26 June 2009 amending
Council Directive 66/401 / EEC, 66/402 / EEC and 2002/55 / ​​EC and 2002/57 / EC as
regards the botanical names of plants, the scientific names of other organisms and certain Annexes
Directives 66/401 / EEC, 66/402 / EEC and 2002/57 / EC
taking into account the development of scientific and technical knowledge.

Implementing Commission Directive 2012/37 / EU of 22 November 2012, which
amending certain Annexes to Council Directives 66/401 / EEC and 66/402 / EEC as regards
about the conditions that must meet seed Galega orientalis Lam.
maximum weight of seed lots of certain species of fodder and sample size
Sorghum spp. Commission Implementing Directive 2013/45 / EU amending Council Directive
2002/55 / ​​EC, Directive 2008/72 / EC and Directive 2009/145 / EC as regards
botanical name for tomato.