219. Regulation of the Federal Minister of Health, amending the poultry hygiene regulations in 2007

On the basis of § 2 (1) to (3) of the Animal Health Act (TGG), BGBl. I n ° 133/1999, as last amended by the Federal Law BGBl. I n ° 142/2003 and the Federal Law BGBl. I n ° 3/2009, will be in agreement with the Federal Minister for Finance and the Federal Minister for Agriculture, Forestry, the Environment and Water Management:

The Poultry Hygiene Regulations 2007, BGBl. II No 100/2007, as last amended by the BGBl Regulation. II No 355/2008, shall be amended as follows:

1. The sequence of words in the table of contents " 7. MAIN ITEM-Special provisions for trade with other Member States of the European Economic Area (EEA)-§ § 44 to 62 " .

2. § 1 para. 3, first sentence reads:

" Provisions of the Food Safety and Consumer Protection Act (LMSVG), BGBl. I n ° 13/2006, the Animal Health Act (TSG), RGBl. No 177/1909, as well as the provisions of the Internal Market Regulation 2008 (BVO 2008), Federal Law Gazette (BGBl). II No 473/2008 and the Veterinary Import Regulation 2008 (VEVO 2008), BGBl. II No 474/2008, remain unaffected. '

3. The previous text of § 2 receives the sales designation "(1)" and the following paragraph 2 is added:

"(2) Any reference to Salmonella typhimurium in this Regulation shall also include monophasic Salmonella Typhimurium with the antigen formula 1.4, [5], 12:."

4. § 7 (1) reads:

" (1) Only water, which is the microbiological requirements of the Drinking Water Ordinance, BGBl, may be used in establishments according to § 1 (1). II No 304/2001, which is the equivalent of the Directive. Proof of this is, unless water is used from a public drinking water supply system, to be provided annually and, if requested, to be submitted to the inspection bodies for inspection by the authorities. "

5. § 9 (2) last sentence reads:

"Samples of the same kind (soil samples, feed nap samples, tear samples, etc.) may be combined into pools of five samples."

6. § 9 (5) reads:

" (5) Stallspaces and areas shall not be allowed until after the completion of cleaning and disinfection in accordance with paragraph 1 and-in the case of para. 2-after the existence of a control result confirming the success of the disinfection measures, but not at the earliest seven days. After the last herd has been left out, it has been refilled with poultry. This period shall be 14 days after measures within the meaning of § 27 or section 42 (6) (determination of a salmonella infection). "

7. In Section 9 (6), the phrase "Regulation (EC) No 1774/2002" through the phrase " Regulation (EC) No 1069/2009 of 21 May 2009. The Commission adopted a proposal for a regulation on animal by-products not intended for human consumption in October 2009 and repealing Regulation (EC) No 1774/1002 (OJ No L 73, 27.2.2009, p. No. L 300 of 14 11. 2009, p. 1) " replaced.

8. § 15 para. 2 reads:

"(2) If animals are obtained from other States, they shall comply with the relevant provisions of the VEVO 2008 or BVO 2008 as appropriate."

9. In § 18, the phrase "Veterinary import and internal market regulation 2001 (EBVO)" through the phrase "BVO 2008" replaced.

10. In § 19 paragraph 1 Z 1 lit. a becomes the phrase "two weeks before entry into the legee phase" through the phrase "two weeks before the transition to the laying phase or the legee unit" replaced.

11. § 19 (1) Z 1 lit. b sublit. bb is:

" bb)				In the case of young animals aged four weeks and in the case of sampling two weeks before the transition to the laying phase or the laying unit, either five pairs of paired boots, each with 20% of the poultry area, shall be sampled, or Co-mixing samples taken from separate samples of fresh cots weighing at least one gram each; samples of feces shall be taken at different points of the room in which the animals are kept; has a sample of the samples taken from the sample. In the same establishment, access to more than one building is free of access, so in each of the poultry farming to remove such samples from the premises of the holding; "

12. § 19 (1) Z 2 reads:

" 2.		Adult Breeding poultry herds (Legephase)
a)			At least every two weeks shall be sampled and examined in all herds during the laying phase.
b)			In herds whose eggs are supplied to hatcheries with a breeding capacity of less than 1000 eggs per breeding pass, and in herds of farmed farms approved for trade within the European Union, the samples shall be taken in accordance with lit. a in the parent animal holding according to the under Z 1 lit. b sublit. bb and cc listed criteria. The sampling in the holding can also be carried out by a person who is verifiably trained by the care veterinarian or by the care veterinarian and by the district managing authority in relation to the named operating person. Every twelve weeks, the sampling shall be carried out by the care veterinarian or by the care veterinarian.
c)			In the case of herds whose eggs are supplied to a hatchery with a gross capacity of at least 1000 eggs per gross pass, the samples shall be in accordance with lit. a is taken in the hatchery. The samples shall include:
aa)				at least one mixed sample of visibly polluted hatching hatching (chicks), which can be taken as a random sample from five different places, until a total area of at least 1 m² is reached (should the hatching eggs be from a breeding heat in several incubators, such a mixed sample is to be taken from each of the incubators), or
bb)				if there are no hordendresses used, a sample of 25 grams taken from a mixture of 10 grams of egg shell residues to be extracted from 25 different hatchery hordes, to be crushed and mixed.
d)			Each herd is in the holding company within four weeks after the stabbing, as well as within eight weeks prior to the planned outpouring after the under Z 1 lit. b sublit. bb and cc are to be officially sampled. Furthermore, in herds according to lit. c in the hatchery every sixteen weeks official samples to be carried out, with the provisions of lit. c should be applied mutatily. In addition, in the in lit. b) During the production cycle of each herd, a further official test is carried out in the 32. up to 40. to make a life week. These probations can in each case replace a test at the instipation of the farmer or the holder of the holding.
e)			The holder of the holding or the farmer shall announce to the District Administrative Authority, when carrying out the activity and in the event of a change to the office of destination (Brüterei), whether the sampling shall be in accordance with lit. b or after lit. c. In the case of implementation according to lit. c is the hatchery to be known. "

13. § 19 (1) Z 3 reads:

" 3.		Investigation of Salmonella gallinarum pullorum (Legephase)
a)			The farmer or the farmer shall, on reaching a lead of at least 10%, carry out an examination of the salmonella gallinarum pullorum by the caregivers 'veterinarian or the caregivers' veterinarian. In the case of male animals, this examination is from the 20th. Week of week. This investigation shall be repeated annually.
b)			In the case of a waste of over 20% or at an incidence rate of 20%, the farmer or the farmer has to carry out an examination of the salmonella gallinarum pullorum by the caregivers 'veterinarian or the caregivers' veterinarian.
c)			The internal organs and intestines of five killed and/or infictitised in five to use animals of the flock immediately before sampling and to investigate bacteriologically. "

14. § 22 (1) reads:

(1) The samples taken from the individual herds may be mixed per herd for analytical purposes, at least two samples of feces being produced; the five paired boot tupfer shall be divided into at least two pools each of which shall be divided into two separate pools each of which shall be divided into two separate pools each of which shall be divided into two separate pools each of a maximum of five individual boots, however, this does not apply to blood samples for serological examination. "

15. § § § 24 to 27:

" Dealing with hatching eggs in case of positive findings

§ 24. (1) Unincubated eggs of a herd, in which the investigations according to § § 19 to 21 of a herd have a positive result within the meaning of § 23 para. 2 on Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum pullorum, Salmonella infantis, Salmonella In the case of the virus, Salmonella hadar or Puten Salmonella arizonae have been obtained,

1.		to be kept separately until the official confirmation of the result in accordance with § 25, or
2.		a treatment according to § 27 Z 1 lit. b, or
3.		in accordance with § 10 of the Animal Material Act, BGBl. I n ° 141/2003, to be destroyed.

(2) The measures referred to in paragraph 1 shall also apply to eggs of these herds already supplied to the brethren. To this end, the Brüterei shall be notified without delay by the farmer of the delivery company via a positive examination report.

Handling of herds in case of positive findings

§ 25. (1) In the case of herds in which the samples according to § § 19 to 21 in the hatchery have a positive result with regard to the presence of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum pullorum, Salmonella infantis, Salmonella virchow and Salmonella hadar, the following shall be taken as follows:

1.		After the notification in accordance with Article 23 (2), the district administrative authority shall, in order to confirm the initial results, immediately subject the stock to an official sampling.
2.		In the case of an official sampling according to Z 1, five pairs of paired boots and two dust samples of 100 g each, each of which are taken at a number of points representative of the stables, shall be drawn in each herd. If no boot samples can be taken on the basis of the superstition system, five samples of 200 to 300 g of faeces in each case in natural mixture of feces, bandscratches or from deep pits can be taken instead. The samples must not be pooled and should be examined for Salmonella in accordance with Z 1. A test for the detection of antimicrobial agents or of an effect of the samples which inhibits the growth of bacteria is to be carried out.
3.		The herd is considered to be positive if a positive result has been found on the salmonella to be studied. The flock is also considered to be positive as long as antimicrobial agents or bacterial growth inhibitory effects are detected. The test should then be repeated until no effect of inhibiting the growth of bacteria is found or the Zuchtherde is destroyed.
4.		The district administration authority may require a reasonable larger sample number if necessary.
5.		In cases of suspiciations in which the district administrative authority has reason to accept false negative results of the first official test, a second official test according to Z 2 may be carried out.

(2) In the case of herds in which the samples according to § § 19 to 21 in the stock (in the stable) have a positive result with regard to the presence of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum pullorum, Salmonella infantis, Salmonella The herd is considered to be positive. If the district administrative authority finds no reasons for the assumption of false positive results, it has the presence of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum pullorum, Salmonella infantis, Salmonella virchow and Salmonella hadar to be officially confirmed. In cases where the district administrative authority has reason to accept false positive results of the probation of the stock, it is necessary to proceed in accordance with paragraph 1 (1) (1) to (5).

(3) In the case of herds in which the samples have shown a positive result in respect of other salmonella in accordance with § § 19 to 21, the herd concerned shall be subject to § 26 in respect of the determination of the source of contamination.

Further investigation

§ 26. In case of suspicion or if it is necessary to determine the source of contamination, the official veterinarian shall:

1.		Carry out investigations into salmonella, including feed, water, litter, dust and operating personnel,
2.		to check whether the rules and controls on the disposal and processing of animal waste have been complied with,
3.		control measures to ensure salmonella-free fodder in accordance with Article 7 (2); and
4.		shall carry out any further appropriate investigation or have it carried out.

Procedure in operation upon confirmation of a salmonella suspicion

§ 27. (1) The occurrence of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum pullorum, Salmonella infantis, Salmonella virchow, Salmonella hadar or Salmonella arizonae in an operating building, or in the case of a review pursuant to § 25, shall be carried out in the case of a review pursuant to § 25. of the corresponding run-out area shall be subject to the following provisions:

1.		The animals may only be brought out of the relevant building with the consent of the district administration authority and only for the following purposes:
a)			Destruction and destruction in accordance with the relevant provisions relating to the use of animal body or
b)			Slaughtering of the clinically healthy poultry in a slaughterhouse designated by the district administrative authority under official supervision in accordance with § 13 of the meat examination ordinance, BGBl. II No 109/2006; the official veterinarian or authorised veterinarian responsible for the slaughterhouse in accordance with the Food Safety and Consumer Protection Act (LMSVG), BGBl. I n ° 13/2005, as last amended by the Federal Law BGBl. I No 135/2006, must be informed of this decision at least three days before the shipment; this must be submitted in accordance with Section 38 (2) of this Decision.
2.		The unincubated eggs from the building concerned are either in accordance with the relevant provisions of Regulation (EC) No 1069/2009 and of the Animal Materials Act (TMG), BGBl. No 141/2003, to be destroyed or, after appropriate labelling, to be put under surveillance at a plant approved for the manufacture and treatment of egg products, where they are in accordance with the requirements of the Regulation (EC) No 853/2004 laying down specific hygiene rules for food of animal origin (OJ L 327, 30.11.2004, p. No. OJ No L 139, 30. 4. 55, as last amended by Regulation (EC) No 2076/2005, OJ L 327, 30.11.2005, p. No. OJ No L 338, 22. 12. 2005) and to Regulation (EC) No 1069/2009 of heat treatment.
3.		In the case of herds who have been stabled in the same premises and in which the investigations in accordance with § § 19 to 21 have not revealed any suspicion, the district administration authority may, with the consent of the district administrative authority, by means of measures in the sense of the Z 1 distance if the veterinary surgeon or the care veterinarian confirms that the herds in question are complete due to their structure, scope and functions in terms of accommodation, posture, care and feeding separate production units, so that the infection in question can not spread from one flock to another. Likewise, in the case of the eggs of the herds mentioned in the first sentence, the measures of the Z 2 can be discernable if the operations ensure that no contamination with infected materials or mixing with eggs is ensured. infected herds can be carried out.

(2) After the removal of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum pullorum, Salmonella infantis, Salmonella virchow, Salmonella hadar or Puten Salmonella arizonae, the plant from the plant is the be subjected to thorough cleaning and disinfection in accordance with the procedure laid down by the official veterinarian in accordance with Article 9 (2). In the case of restovings with poultry, it is necessary to ensure that this corresponds to the conditions of § 15.

(3) § 34 shall apply to hatching eggs already supplied to a hatchery, from a herd where the suspicion of infection is confirmed.

(4) On the basis of the results of investigations in accordance with § 26 for the determination of the source of contamination, the District Administrative Authority may, if necessary, determine by means of communication work regulations for the maintenance of a new infection. "

16. In Section 28 (1) (1) (1), the phrase "according to § 25 Abs.1 Z 2" through the phrase "according to § 25" replaced.

17. In Section 28 (2) and Section 34 (1), the word order shall be "Regulation (EC) No 1774/2002" through the phrase "Regulation (EC) No 1069/2009" replaced.

Section 30 (3) reads as follows:

"(3) If hatching eggs, chicks or young animals are obtained from third countries, the suitability for the purposes of para. 1 and 2 shall be proved by the submission of a cross-border veterinary clearance certificate in accordance with § 33 VEVO 2008."

19. In Section 33 (2), the phrase "subject to the seventh main piece" through the phrase "from companies which are registered in accordance with § 13 BVO 2008 or registered in accordance with § 15 BVO 2008" replaced.

20. § 37 (1) last sentence reads:

" In the case of slaughtering of the herd in several lots, this study should be repeated in the animals still to be slaughtered if a part of the herd is later than 36 days-in the case of broilers and turkeys with an age of less than one hundred days (except Pute from a biological position within the meaning of Regulation (EC) No 889/2008) shall be slaughtered later than 21 days after the test. "

21. In Section 37 (2), the word order shall be "lit. a" through the phrase "Z 1" replaced.

22. § 37 (5) deleted.

23. § 37 (6) Z 5 reads:

" 5.		the date, time and results of the data referred to in paragraph 1, (2) Salmonella controls carried out, "

24. § 37 (6) (6) (6).

25. § 37 (7) reads:

" (7) The provincial governor has official sampling for fattening fowl of Gallus gallus at least in accordance with the provisions of Regulation (EU) No 200/2012 on a Union target for the reduction of Salmonella enteritidis and Salmonella typhimurium Broiler herds referred to in Regulation (EC) No 2160/2003 of the European Parliament and of the Council (OJ L 284, 31.7.2003, p. OJ L 71 of 9. 3. 31). "

Section 38 (2) Z 4 reads as follows:

" 4.		for consignments of poultry which are obtained from other countries, a certificate corresponding to the provisions of the VEVO 2008 or BVO 2008. "

27. § 39 paragraph 1 Z 1 lit. a is:

" (a)			the sampling shall be carried out in accordance with section 41 (2); "

28. In § 39 paragraph 1 Z 1 lit. b becomes the phrase " in the 14. to 18. Week " through the phrase "two weeks before the transition to the laying phase or the laying unit".

29. Before § 40, first sentence, the sales designation shall be "(1)" inserted.

30. § 41 (1) to (3) are:

" (1) All laying hens which are subject to this Regulation and which are also registered in accordance with the marketing standards for eggs, BGBl II No 365/2009, shall be required to have each herd examined for salmonella in an approved laboratory.

(2) The probation of all the laying hens shall be carried out at the instition of the farmer or the farmer at least every 15 weeks by the care veterinarian or by the care veterinarian, the first-time trial at the age of 22 up to 26 weeks.

1.		In the case of herds in cages, a total of 60 places can be taken from two collection samples of 150 g of fresh food, either from all the fender bands/scrapers, after the demisting plant has been operated, or from the coke rubs.
2.		In the case of soil, free-land or volites, two pairs of paired boots are to be taken.
3.		In the case of holdings with several small herds with a total of up to 2000 animals forming an epidemiological unit, all herds may also be jointly tested at different ages, with a separate pair of boots for each flock. is used. The required initial test in the 22. up to 26. However, it is important to observe a maximum interval of 15 weeks for each herd. The decision as to whether or not several herds are to be sampled as an epidemiological unit shall be notified without delay to the relevant district administrative authority.

(3) An official sampling of all laying hen's herds shall be carried out at least once a year, in addition to the requirements listed in paragraph 2 (2) (1) and (2) of this Regulation.

1.		a sample of dust must be drawn to 100 g of a number of points representative of the stabling, or
2.		Samples with one or more damp dampers with a total surface area of at least 900 cm² are drawn from many places in the barn, ensuring that each wiper is well covered with dust on both sides, and
3.		in any case, a sample of the sample at 60 points representative of the stabling shall be drawn to a total of 150 g.
Tests for the detection of antibiotics and antimicrobial effects in the laboratory are to be carried out on the samples according to Z 1 to 3. "

31. § 42 reads:

" § 42. (1) If a sample has a positive effect on salmonella or antibiotics or anti-microbial effects in accordance with § 41, this shall be done in the case of an operational control by the care veterinarian or by the care veterinarian and in the case of to report official control by the official veterinarian or the official veterinarian of the district administration. In the event of a positive result of the examination for antibiotics or antimicrobial effects, the Landeshauptmann shall immediately be responsible for any reason within the meaning of Section 13 of the residue control regulation, BGBl. II No 110/2006.

(2) If there is a positive result for Salmonella enteritidis or Salmonella typhimurium, the farmer or farmer shall have the following:

1.		to check the hygiene measures in accordance with the second main item of this Regulation by the veterinary surgeon or the care veterinarian and to have it recorded in accordance with the requirements of the Austrian Poultry Health Service, and
2.		to draw up a recovery plan based on a vulnerability analysis.

(3) If it is confirmed by the Austrian reference laboratory for salmonella that a positive result is a vaccine strain, and if the competent authority has not been brought to the attention of human diseases with that strain, the following shall be: may be removed from measures within the meaning of paragraph 2 and 4, but the herd shall be officially sampled after a period of three weeks.

(4) If a sample in accordance with § 41 results in a positive finding on Salmonella enteritidis or Salmonella typhimurium or by the farmer or the farmer, respectively, where the farmer has not complied with the provisions of this Regulation, the following shall apply:

1.		the eggs are subject to Annex II D Z 2 of Regulation (EC) No 2160/2003 on the control of salmonella and other specified food-borne zoonotic agents, OJ L 327, 31.12.2003, p. No. 1), as last amended by Regulation (EU) No 1086/2011 amending Annex II to Regulation (EC) No 2160/2003 of the European Parliament and of the Council and Annex I to Commission Regulation (EC) No 2073/2005 in respect of salmonella in fresh poultrymeat, OJ L 327, 28. No. OJ L 281, 28.10.2011, p.7.;
2.		in the case of the flock concerned, the Austrian poultry health service database shall immediately enter into the ban on the marketing of fresh eggs in accordance with the marketing standards for eggs;
3.		the competent district administrative authority shall establish the existence of the marketing ban of fresh eggs with a communication with the farmer or the farmer, and the veterinary surgeon or the care veterinarian, as well as the to inform the competent food authority thereof;
4.		in the case of the exclusive detection of antibiotics or anti-microbial effects, the official sampling must be repeated immediately.

(5) If the district administrative authority has the reasonable suspicion of false positive results of the investigations in accordance with § 41, the marketing restriction according to paragraph 4 Z 1 may be lifted if it is to be carried out from another immediately official sampling

1.		a negative result from the studies referred to in Annex II D Z 4 of Regulation (EC) No 2160/2003, and
2.		a negative result of tests for the detection of antibiotics and antimicrobial effects in the laboratory from a collection sample to 60 sites representative of the stables to a total of 150 g is available.

(6) A substantiated suspicion pursuant to paragraph 5 may also be made by the farmer or the holder. be expressed by the farmer to the district managing authority, but only if

1.		the operation in the previous 24 months was not affected by the same type of salmonella serotype,
2.		the last official control in accordance with § 14 has not been subject to any complaint concerning hygiene conditions; and
3.		in the case of membership of the poultry health service, the animal health service surveys which have been carried out since the previous official control do not have any unresolved complaints in the areas of hygiene, animal health status and Management/attitude (points 2, 3. and 6. of the QGV-Operating Survey Protocol).
For the possibility of a cancellation of the marketing restriction pursuant to paragraph 4 (1) (1) following this suspicion, paragraph 5 shall apply mutatily.

(7) Where an official sampling is carried out in the context of the declarations of outbreak in accordance with the Zoonoses Act, BGBl. I n ° 128/2005, which confirms the epidemiological link with outbreaks of disease caused by salmonella infection in humans, the district administration authority has to order a killing of the herd. For eggs from this herd shall be valid until the date of killing (4).

(8) The second main item of the Animal Health Act shall apply to the compensation of the animal owners in the case of an order for a killing as provided for in paragraph 7. "

32. The 7. The main piece is omitted.

33. In accordance with Section 64 (3), the following paragraph 4 is added:

" (4) § 1 para. 3, § 2, § 7 para. 1, § 9 para. 2, 5 and 6, § 15 para. 2, § 18, § 19 para. 1, § 22 para. 1, § § 24 to 28, § 30 para. 3, § 33 para. 2, § 34 para. 1, § 37 para. 1, 2, 6 and 7, § 38 paragraph 2 Z 4, § 39 paragraph 1 Z 1, § 40 para. 1, § 41 para. 1 to 3, § 42 as well as Annex A and Annex B in the version of the BGBl. II No. 219/2013 will enter into force on 1 November 2012. "

34. Annex A and B are:

" Annex A

Laboratories approved by the Federal Minister of Health for the examination of official samples:

all approved laboratories of the AGES

Landesanstalt für veterinary examinations Klagenfurt

Laboratories approved by the Federal Minister of Health for the investigation of Salmonella spp.:

all approved laboratories of the AGES

the Laboratory of Poultry Clinic of the University of Veterinary Medicine, Vienna

Landesanstalt für veterinary examinations Klagenfurt

Annex B

Sampling and sampling

1. Inquiry into animal feed

In accordance with § 26 of the 2007 poultry hygiene regulations, in case of suspicion or if it is necessary to determine contamination sources, feed on salmonella according to the indicated method is to be investigated:

Salmonella spp.: Examination of 5 samples of 25 grams each of the feed in the culture process according to recognised methods (validated ISO method) ISO 6579, accredited laboratory.

Sampling shall be carried out with particular regard to microbial contamination in accordance with the provisions of Regulation (EC) No 152/2009.

2. sampling provisions by step-by-step tests in soil, open-land or voolis neck:

-	Boot pads are commercially available absorbent fabric boots and cover the entire shoe undersurface including parts of the shoe top of the/or the shoe upper. of the sample drawing. The surface of the boot tupfer must be moistened with a suitable diluent (e.g. with sodium chloride 0.8% or Pepton 0.1% which is dissolved in sterile ionised water, with sterile water without additives or with any other water) Diluent which has to be approved by the competent authority).
-	As sample sets, either the sample sets made available by the Austrian Poultry Health Service will be used, respectively. shall be able to draw on the sample sets to be kept in the same way.
-	After entering the barn, new plastic boots are to be tightened and then only the boot pads are to be applied. In this case, gloves are to be used. The boot pads themselves must not come into contact with disinfectants.
-	The bottom surface of the barn is to be divided into two equal parts for the sample operation. When walking around the stable, at least 100 steps should be completed with each pair of boot tupers so that samples are collected in all parts, including areas with and without litter or slats, if these are safely accessible. All separate bays of a stable must be included in the test, with the exception of outdoor areas for free-land holding.
-	After the end of the rehearsals the boots are to be carefully removed with the gloves, so that adhering material does not dissolve and to be inserted into the transport plastic bag tightly sealed to the corresponding laboratory

Sampling provisions for cages:

In the case of herds kept in cages, two samples of 150 g each of naturally mixed faeces must be taken from all the faeces in the interior of the stables after the demisting plant has been activated, whereas the samples shall be kept in stuf cage stables, which shall be used for the purpose of: are not equipped with fender conveyor belts or belt scrapers, at 60 different places from the feces below the cages two samples of 150 g each are to be taken from fresh mixed faeces.

4. Sample provisions for dust-wipe-test specimens

For the dust sample, one or more humidified towel banks with a total surface area of at least 900 cm 2 are used to collect dust from different surfaces with visible dust deposition throughout the barn, while ensuring that each swab is well covered with dust on both sides.

5. Laboratory provisions

The germs to be studied shall be examined for the following microbiological parameters in accordance with the methods indicated:

Salmonella enteritidis	Culture test according to recognised procedures (validated ISO/OIE method) ISO 6579 Annex D, accredited laboratory
Salmonella Typhimurium	Culture test according to recognised procedures (validated ISO/OIE method) ISO 6579 Annex D, accredited laboratory
Salmonella gallinarum pullorum	Culture test according to accepted methods (validated ISO/OIE method) accredited laboratory
Salmonella arizonae	Culture test according to recognised procedures (validated ISO/OIE method) ISO 6579 Annex D, accredited laboratory

6. Special provisions for the investigation of salmonella

a) The samples shall be sent to the laboratory within 24 hours after sampling. If the delivery is not carried out within 24 hours, the samples shall be stored in a cool place. The samples can be transported at room temperature, provided that excessive heat (above 25 ° C) and solar radiation are avoided. In the laboratory, samples shall be stored in a cool place until examination, which shall be carried out within 48 hours of the receipt and within four days of sampling.

b) The boots cover and dust or Dust swabs are to be prepared separately. The two pairs of boots, respectively the dust tupfer must be carefully packed to ensure that the material of the faecal material adhering to it, or the dust does not dissolve, and put together in at least 225 ml of buffered peptic water (BPW), which has been warmed to room temperature. The boot-overdral or Dust swabs must be completely immersed in peptonwater, and in any case there must be so much liquid that the salmonella can move away from the sample.

In order to completely saturate the sample, it is to be pivoted; the examination of the pre-enrichment medium inoculated with the swab is then continued by means of the method indicated in the table (according to Annex D to the standard ISO 6579 (2002)).

c) Other faecal material and dust samples:

The faecal samples are to be put together and thoroughly mixed. For the purpose of the application of cultures, this mixture is to be taken from a subsample of 25 grams.

The subsample of 25 grams should be added to 225 ml BPW, which has been preheated to room temperature. The examination shall then be carried out in accordance with the method referred to in the table (in accordance with Annex D to ISO 6579 (2002)).

d) From each positive finding, an isolate is to be sent to the National Reference Laboratory for Salmonella and to be typed according to Kaufmann-White-Scheme.

e) The strains isolated in the course of official controls shall be stored for subsequent phagotyping or testing for susceptibility to antimicrobials in accordance with the usual methods of cultural collection, the integrity of which shall be: trunks for at least two years. "

Stöger