Royal Decree 767/2014 of 12 September 2014 establishing the title of Superior Technician in Pathological and Diagnostic Anatomy and setting out its minimum teachings, acts in accordance with Royal Decree 1147/2011 of 29 In July, the General Management of Vocational Training in the Education System, which defines in Article 9 the structure of vocational training qualifications and courses of specialisation, is established on the basis of the Catalogue of Vocational Training and Vocational Training. National of Professional Qualifications, the guidelines set by the European Union and others aspects of social interest, and implements the aforementioned title of Pathological and Cytodiagnostic Anatomy.

Organic Law 2/2006, of 3 May, of Education, provides that educational administrations will develop the curriculum of vocational training diplomas, starting with the basic curriculum and under the conditions laid down in the Article 6 bis.4 The teaching centres shall develop and complete, where appropriate and as established by the educational authorities, the curriculum of the different stages and cycles in use of their autonomy as set out in Chapter II of Title V of the said Directive. Organic Law.

Royal Decree 767/2014 of 12 September 2014 in its Single Derogation Provision repeals Royal Decree 550/1995 of 7 April 1995 establishing the curriculum of the higher education cycle corresponding to the title of the Superior Technician in Pathology and Pathology, established under the Organic Law 1/1990, of October 3, of General Ordination of the Educational System.

In accordance with the above and once the Royal Decree 767/2014 of 12 September has set the professional profile of the title of Superior Technician in Pathological and Diagnostic Anatomy, the basic aspects of the curriculum and other aspects of the academic organisation which ensure a common training and guarantee the validity of the titles throughout the national territory, it is now appropriate to determine, in the field of management of the Ministry of Education, Culture and Sport, the extension and contextualisation of the content of the professional modules included in this title, respecting the professional profile of the same.

The needs of an integrated labour market in the European Union require that vocational training courses pay particular attention to the languages of the Member States, incorporating them into their training offer. In this sense, this formative cycle incorporates in the curriculum training in the English language, in response to the provisions of Royal Decree 1147/2011, of July 29.

addition, the curriculum of this training cycle is established from the respect of the pedagogical, organizational and management autonomy of the centers that provide professional training, promoting these the work in the team of the teachers and the development of training, research and innovation plans in their teaching field and actions to promote the continuous improvement of training processes.

On the other hand, vocational training centres will develop the curriculum established in this order, taking into account the characteristics of pupils, with particular attention to the needs of people with disability.

Finally, it should be specified that the curriculum of this formative cycle integrates the scientific, technological and organizational aspects of the teachings established to achieve that the students acquire an overall view of the processes production of the professional profile of the superior technician in Pathological and Cytodiagnostic Anatomy.

In the process of drafting this order, the State School Board has issued a report.

For all of the above, in its virtue, I have:

CHAPTER I

General provisions

Article 1. Object.

This order is intended to determine, from the basic curriculum set out in Royal Decree 767/2014 of 12 September, establishing the title of Superior Technician in Pathological and Diagnostic Anatomy and set out its minimum lessons, the curriculum of the higher grade training cycle corresponding to that title.

Article 2. Scope.

The curriculum established in this order will be applied in the territorial area of management of the Ministry of Education, Culture and Sport.

CHAPTER II

Curriculum

Article 3. Curriculum.

1. The curriculum for vocational training of the educational system corresponding to the title of Higher Technician in Pathological and Diagnostic Anatomy, set out in Royal Decree 767/2014 of 12 September, is determined in the terms set in this order.

2. The professional profile of the curriculum, which is expressed by the general competence, the professional, personal and social skills, and the qualifications and the competence units of the National Catalogue of Professional Qualifications, is the included in the title of Superior Technician in Pathological and Cytodiagnostic Anatomy, referred to in the previous section.

3. The general objectives of the training cycle curriculum, the objectives of the professional modules expressed in terms of learning outcomes and their evaluation criteria are those included in the title of Higher Technician in Anatomy Pathological and Citodiagnostic referred to in paragraph 1 of this Article.

4. The contents of the professional modules that make up this curriculum, adapted to the socio-economic reality as well as to the perspectives of economic and social development of the environment, are those set out in Annex I of this order.

Article 4. Adaptation to the socio-productive environment.

1. The curriculum of the training cycle regulated in this order is established taking into account the socio-economic reality and the geographical, socio-productive and labour characteristics of the environment for the implementation of the title.

2. Vocational training centres shall have the necessary educational, organisational and economic management autonomy for the development of the teaching and their adaptation to the specific characteristics of the socio-economic, cultural and professional.

3. The centres authorised to provide this training cycle will concretize and develop the organizational and curricular measures that are most appropriate to the characteristics of their students and their productive environment, in a flexible way and in the use of their Pedagogical autonomy, in the general framework of the educational project, in the terms established by the Organic Law 2/2006, of Education.

4. The curriculum of the training cycle regulated in this order will be developed in the didactic programs or curricular development, strengthening or creating the culture of prevention of occupational risks in the spaces where the different modules are delivered professionals, as well as promoting a culture of environmental respect, excellence in work, compliance with quality standards, creativity, innovation, gender equality and respect for equal opportunities, the " design for all people " and universal accessibility, especially in relation to people with disabilities.

Article 5. Adaptation to the educational environment.

1. The vocational training centres managed by the Ministry of Education, Culture and Sport will develop the curriculum established in this order, taking into account the characteristics of pupils and the environment, particularly in the light of persons with disabilities, in conditions of accessibility and with the necessary support resources to ensure that this student can cure these teachings under the same conditions as the rest.

2. Also, the lessons of this cycle will be taught with a flexible and open methodology, based on self-learning and adapted to the conditions, abilities and personal needs of the students, in order to allow the reconciliation of the learning with other activities and responsibilities.

Article 6. Duration and sequencing of professional modules.

1. The total duration of the lessons for this training cycle, including the vocational training module in the workplace, is 2,000 hours.

2. The professional modules of this training cycle, when offered under face-to-face arrangements, will be organised in two academic courses and will be in line with the weekly hourly sequencing and distribution set out in Annex II of this order.

3. The first academic year will be fully developed in the educational center. In order to be able to pursue the second course, it will be necessary to have completed the professional modules which, as a whole, are at least 80% of the hours of the first course.

4. The right of registration of those who have passed any professional module in another Autonomous Community shall be guaranteed in the terms laid down in Article 48.3 of Royal Decree 1147/2011 of 29 July 2011 establishing the general vocational training of the educational system.

5. In general, during the third quarter of the second year, and once the positive evaluation has been achieved in all the professional modules carried out in the educational centre, the vocational training module will be developed in job.

6. Exceptionally, and in order to facilitate the adaptation of the number of persons registered to the availability of training positions in enterprises, approximately half of the second-year students will be able to develop this vocational training module. Training in workplaces during the second trimester of the second year, provided they have positively overcome all the professional modules of the first academic year.

7. Without prejudice to the foregoing and as a result of the temporality of certain economic activities which may prevent the development of the vocational training module in the workplace from being in conformity with the above assumptions, the latter may be organise in other periods coinciding with the development of the economic activity of the professional profile of the title.

8. In any case, the evaluation of the vocational training module in the workplace will be conditional on the positive evaluation of the rest of the professional modules of the training cycle.

Article 7. Project Professional Module.

1. The project professional module has an interdisciplinary character and incorporates the technological and organizational variables related to the essential aspects of the professional competence of the title of Superior Technician in Pathological Anatomy and Cytodiagnosis.

2. In general, this module will be taught by the teachers who are tutoring training in job centers.

3. The project professional module will be developed during the last period of the training cycle, combining individual and collective tutoring, so that at least 50% of the total duration will be carried out in an in-person manner and completed with remote tutoring in which information and communication technologies will be used.

4. In any case and prior to the start of the vocational training module in the workplace, the teaching and learning activities to facilitate the development of the project professional module should be anticipated by the responsible teacher.

5. The evaluation of this professional module will be conditional on the positive evaluation of the rest of the professional modules of the training cycle, including the training in job centers.

Article 8. Bilingual teaching.

1. The curriculum of this training cycle incorporates the English language in an integrated way, at least in two professional modules, from among those that make up the whole of the training cycle. These modules will be taught by teachers with teaching assignment in them and, in addition, they will have the language enablement corresponding to level B2 of the Common European Framework of Reference for Languages.

2. In order to ensure that bilingual education is delivered in the two academic courses of the training cycle on an ongoing basis, professional modules of both courses will be chosen.

3. The modules which may be imparted in the English language are those listed in Annex III.

4. As a result of the greater complexity of the transmission and reception of teaching in a language other than the mother tongue, professional modules taught in the English language will increase their time load in three hours a week. for the set of modules that are delivered in the first year and two hours for which they are developed during the second course. In addition, the teachers who provide these professional modules will be assigned, in their individual hours, at least three hours a week for their preparation. These hours will have the same character as the reading hours.

5. Exceptionally and on a transitional basis up to the year 2020, when teachers with teacher allocation do not have the level of English required in these professional modules, they will share a total of three hours per week for all the teachers. modules to be delivered in the first year and two hours for those who develop during the second course with a teacher or a teacher of the English specialty. In this case, the programming of these modules will include at least one unit of work or didactics which will be developed exclusively in the English language and the other teaching units will incorporate teaching activities exclusively in English at that time allocated.

6. Exceptionally, in the case of students or students with disabilities who may have difficulties in their oral expression (cerebral palsy, deafness ...), more flexible and/or alternative measures will be put in place. the requirement for the provision of modules in the English language, so as to enable all the teaching of professional modules in the Spanish language. These adaptations shall in no case be taken into account in order to undermine the qualifications obtained.

CHAPTER III

Teachers, spaces and equipment

Article 9. Qualifications and accreditation of teacher requirements.

1. The specialties of teachers with teaching assignment in the professional modules which constitute the teachings established for the title referred to in Article 1 of this order, as well as the equivalent qualifications for the purposes of teaching, are the entries respectively in Annexes III A and III B to Royal Decree 767/2014 of 12 September 2014.

2. In order to ensure compliance with Article 12.6 of Royal Decree 767/2014 of 12 September 2014, for the delivery of the professional modules that make up the company, in private or public ownership centers of other administrations other than educational administrations, it must be established that all the requirements set out in that Article are met, with the following documentation:

(a) Photocopy of the official academic title required, in accordance with the qualifications included in Annex III C of Royal Decree 767/2014 of 12 September. Where the certificate submitted has direct correspondence with the professional module to be delivered, it shall be deemed to include in itself the objectives of that module. Otherwise, in addition to the titration, the documents referred to in subparagraph (b) or (c) shall be provided.

(b) In the event that it is necessary to justify that the teachings leading to the titration provided encompass the objectives of the professional modules that are intended to be taught:

Personal academic certification of studies performed, original or photocopied, issued by an official center, in which the teachings are recorded detailing the subjects.

Programs of the studies provided and submitted by the person concerned, original or photocopy of the studies, sealed by the corresponding official or authorized official or official University or Center.

c) Where it is necessary to justify by means of the work experience which, at least for three years, has developed its activity in the sector related to the family, its duration shall be credited by the appropriate supporting official document, which shall be added to:

Certification of the employer or employer in which the activity developed by the person concerned is specifically recorded. This activity must be implicitly related to the learning outcomes of the professional module that is intended to be delivered.

For those who are self-employed, a statement of the person concerned with the most representative activities related to learning outcomes.

Article 10. Spaces and equipment.

The spaces and facilities to be assembled by the vocational training centres, in order to enable the development of teaching activities, are those set out in Annex IV of this order and must comply with the Article 11 of Royal Decree 767/2014 of 12 September 2014, as well as the rules on equal opportunities, "design for all persons" and universal accessibility, prevention of occupational risks and safety and health at the workplace.

CHAPTER IV

Other offerings and mode of these teachings

Article 11. Distance offering.

1. Professional modules offered at a distance, when required by their characteristics, will ensure that students achieve all the objectives expressed in learning outcomes, through face-to-face activities.

2. The Provincial Directorates and the Board of Education shall take the necessary measures and shall give the precise instructions to the centres which are authorized to provide this training cycle under pressure for implementation and operation of the offer from the same distance.

3. Centres authorised to provide vocational training lessons at a distance shall have appropriate curriculum materials which will be adapted to the provisions of the fourth additional provision of the Organic Law 2/2006 of 3 May.

Article 12. Combined offering.

In order to respond to personal needs and interests and to provide the possibility to reconcile training with work activity, with other activities or situations, the offer of these teachings for people adults and young people in special circumstances may be combined between face-to-face and distance learning systems at the same time, provided that the same modules are not cured in both modes at the same time.

Article 13. Offer for adults.

1. The professional modules of this training cycle associated with the competence of the National Catalogue of Professional Qualifications may be the subject of a modular offer for adults.

2. This training will be developed with an open and flexible methodology, adapted to the conditions, capabilities and personal needs that enable them to reconcile learning with other activities and responsibilities, in compliance with the Chapter I of Title IV of Royal Decree 1147/2011 of 29 July 2011. In addition, such training shall be capitalizable in order to obtain a professional training certificate, in order to obtain evidence of the established access requirements.

3. In order to reconcile learning with other activities and responsibilities, the Provincial Directorates and the Board of Education may establish specific measures to comply with the provisions of Article 41 of Royal Decree 1147/2011, July 29, and enable a face-to-face and remote offering simultaneously.

4. In order to promote training throughout life, the Directorate-General for Vocational Training of the Ministry of Education, Culture and Sport may authorise the Provincial Directorates and the Education Ministry to give the training, in the centres of their competence, of professional modules organised in training units of shorter duration. In this case, each learning result, with its evaluation criteria and its corresponding block of contents, will be the minimum and indivisible unit of partition.

Additional disposition first. Authorization to impart these teachings.

The Provincial Directorates and the Board of Education will deal with the Directorate-General for Vocational Training with the authorization to provide the teaching of this training cycle, in full or in part, in In-person and distance-based arrangements, of the institutions that request it and comply with the requirements required under the current legislation.

Additional provision second. Linguistic enablement of bilingual teaching faculty.

Teachers who are to be taught in English must be in possession, before the date of commencement of each academic year, of the relevant language qualification, to which the Ministry of Education, Culture and Sport will carry out an enabling procedure before the start of each course.

Additional provision third. Training of bilingual teaching staff.

The Provincial Directorates and the Board of Education will schedule courses and training activities in the English language for all teachers of vocational training to be taught in professional modules. which may be taught in the English language, who will have the obligation to assist them until they obtain the required qualification. These measures shall apply until at least the year 2020.

The training to be offered will be three types:

a) Intensive training, through a course, preferably in face-to-face mode, during the month of September.

b) Long-term training throughout the school year, by means of a course that combines in-person and online form, which will be performed outside of the mandatory time in the training center. During the period of completion of the vocational training module in the workplace, this course will be intensified and will be carried out, as far as possible, within the required time of stay in the centre.

(c) Training in English-speaking country, through courses, which will be possible to include cultural visits and conferences, and which will be carried out at the end of the course after the completion of school activities in the training centres.

Single transient arrangement. Replacement of titles related to these teachings.

1. The student who, at the end of the school year 2015 -2016, meets the conditions required to pursue the second course of the title regulated in Royal Decree 538/1995, of April 7, for which the title of Superior Technician is established in Anatomy Pathological and Citology and the corresponding minimum teaching, under the Organic Law 1/1990 of 3 October, of General Management of the Educational System, and which has not exceeded any of the professional modules of the first course of the mentioned title, will have two calls in each of the two successive years to be able to overcome these professional modules. After that period, in the 2018-2019 school year, the convalidations will apply to the superimposed modules, as set out in Article 15.1 of Royal Decree 767/2014, of 12 September, governed by the Organic Law 2/2006, of 3 of May.

2. The students who, at the end of the school year 2015 -2016, do not meet the conditions required to attend the second course of the title of Superior Technician in Pathological Anatomy and Citology, established by Royal Decree 538/1995, of April 7, to the Under the Organic Law 1/1990 of 3 October, the convalidations set out in Article 15.1 of Royal Decree 767/2014, of 12 September, governed by the Organic Law 2/2006, of 3 May, will apply.

3. The students who, at the end of the 2016-2017 school year, do not meet the conditions required to obtain the title of Superior Technician in Pathological Anatomy and Citology, established by Royal Decree 538/1995, of April 7, under the Law Organic 1/1990, of 3 October, will have two calls in each of the two successive years to be able to overcome these professional modules, with the exception of the training module in the center of work for which a school year will be available supplementary. The students who have passed that period have not obtained the title will be applied to the convalidations, for the modules surpassed, established in article 15.1 of the Royal Decree 767/2014, of 12 September, regulated by the Organic Law 2/2006, of 3 May.

Final disposition first. Application of the order.

The Directorate-General for Vocational Training, in the field of its powers, is authorised to take the measures and to issue the necessary instructions for the implementation of the provisions of this order.

Final disposition second. Implementation of these teachings.

1. In the course of 2015-2016, the first course of the training cycle referred to in Article 1 of this order will be implemented and the first course lessons covered by the Organic Law 1/1990 of 3 October will cease to be taught. corresponding to Royal Decree 538/1995 of 7 April.

2. In the course of 2016-2017 the second course of the training cycle referred to in Article 1 of this order will be implemented and the second course lessons covered by the Organic Law 1/1990 of 3 October will cease to be taught. corresponding to Royal Decree 538/1995 of 7 April.

Final disposition third. Entry into force.

This order will take effect the day following your publication in the "Official State Bulletin".

Madrid, 21 July 2015.-The Minister of Education, Culture and Sport, Iñigo Méndez de Vigo and Mr Mondez.

ANNEX I

Professional Modules

1. Professional Module: Managing biological samples.

Code: 1367.

Contents:

a) Analysis of the organizational structure of the healthcare sector:

Healthcare systems. Types. Healthcare systems in Spain.

Legislation on the Law on the Management of Health Professions: General Law of Health.

Functions, areas, and organization of work in the clinical analysis laboratory and pathological anatomy:

Functions of clinical laboratory technicians.

Functions of pathological anatomy technicians.

Health economics and quality of service delivery.

b) Identification of the lab documentation:

Receiving, recording, and classifying samples.

Documentation management computer systems.

Bioethics regulatory documents.

Organic Data Protection Law. Informed consent.

Record and graphical documentation file.

Budgets, procurement and administration of supplies and warehouse control.

c) Identification of biological samples:

Biological samples.

Liquid samples.

tissue samples.

Cytological samples.

anatomical characteristics of the extraction region.

Analyzable substances:

Qualitative and quantitative analysis.

Preanalytic variability of the patient.

Errors in preanalytic manipulation.

Gender. Health and disease.

d) Realization of the collection, according to the protocol of the unit, and distribution of common biological samples:

Materials used for sample extraction.

Blood samples:

Types of blood samples.

Blood extraction techniques. Venous extraction in anatomical model.

Anticoagulants.

Non-blood samples:

Urine sample.

Samples of digestive origin.

Samples of the male and female reproductive system. Gynaecological cytology.

Mom: Sections and Puntions.

Intraoperative Cytology by Imprompt.

Exudates for microbiological-parasitic analysis.

Skin samples for the study of mycoses: skin, hair and nails.

Basic life support techniques.

e) Realization of the collection, according to the protocol of the unit, and distribution of samples obtained by invasive or surgical procedures:

Obtaining samples in anatomical structures and viscera:

Fine needle (PAAF) and thick needle (BAG).

Puncture Gun (cameco).

Imprint and scraping.

Image technology resources for obtaining samples.

Types of samples obtained by invasive or surgical procedures:

cerebrospinal fluid (CSF).

Serous and exuded liquids.

Respiratory tract samples.

Samples obtained from experimental animals.

Biobank samples.

Service Delivery Process. The unit's performance protocols.

f) Selection of conservation, storage, transportation, and shipment of samples:

Samples conservation criteria: factors that affect the preservation of samples.

Sample preservation methods:

Chemical methods.

Physical methods.

Packaging, shipping, and shipping systems. Current regulations.

Record, encoding, and identification of the sample for transport.

g) Application of safety protocols and risk prevention in the handling of chemical and biological products:

Chemical, radioactive and biological reagents. Storage. Incompatible chemical substances.

Prevention of the risk of work with chemicals, radioactive and biological products:

Gas and biosecurity cabins.

Manipulation of products.

Prevention of risks related to laboratory equipment.

Waste Management. Current regulations.

Determination of prevention and personal protection measures.

Protocol of action to an emergency situation. Emergency plan.

Organization of preventive work. Basic routines.

Documentation: Collecting, crafting, and archiving.

2. Professional Module: General laboratory techniques.

Code: 1368.

Contents:

a) Classification of materials, basic equipment and reagents:

Material types and usage:

Volumetric material.

Micropipettes.

Basic Laboratory Utensils and their use.

Cleaning, disinfection and sterilisation of the laboratory material.

The lab water.

Chemical Reagents in the Clinical and Pathological Laboratory:

Classification and tagging.

Handling, storage, and storage.

Security Tokens.

Basic equipment used in the lab.

Efficient use of resources.

Standard procedures for work.

b) Application of safety protocols and risk prevention in the laboratory:

Chemical, radioactive and biological reagents. Storage. Incompatible chemical substances.

Prevention of the risk of work with chemicals, radioactive and biological products:

Gas and biosecurity cabins.

Manipulation of products.

Prevention of risks related to laboratory equipment.

Waste Management. Current regulations.

Determination of prevention and personal protection measures.

Protocol of action to an emergency situation. Emergency plan.

Organization of preventive work. Basic routines.

Documentation: Collecting, crafting, and archiving.

c) Realization of solutions and dilutions:

Mass measurements using precision scales:

Basis and rules of use.

Accuracy, precision, sensitivity, and load capacity.

Volume measures by volumetric material:

Use of volumetric material.

Accuracy and precision.

Calculation and preparation of solutions:

Mode of expression of the concentration. Calculation and units.

Preparation of solutions.

Calculation and preparation of dilutions: concept and forms of expression.

Preparing serial and unserialized dilutions.

Electrochemical methods: the pH-meter:

Types of electrodes.

Calibrated, measurement, and maintenance.

Acid-base valuations. Preparation of buffer solutions.

d) Application of substance separation procedures:

Basic separation methods. Filtration, decantation and centrifugation.

Electrophoretic Separation Methods:

Application of electrophoretic techniques.

Preparing equipment, reagents, and maintenance.

Interpretation of instrumental analysis results:

Statistical treatment of results for quality control.

Digital reporting.

e) Realization of the technical assessment of the consistency and reliability of the results:

Basic statistical concepts: mean, standard deviation, coefficient of variation and regression.

Quality control in the analytical phase. Calibration and control material.

Analytics string: Error types.

Quality Control Graphical Representations.

Acceptance or rejection criteria.

f) Realization of microscopy and image scanning techniques:

Basic components of an optical microscope and photo equipment.

Transmitted light optical microscopy techniques. Basis and application of each of them.

fluorescence microscopy techniques. Applications and benefits of each technique.

Electronic microscopy techniques. Foundation and application.

Probe scanning microscopy techniques. Foundation and application.

Macroscopic, microscopic and ultramicroscopic photographic techniques.

Digital image capture, processing, and file systems:

Digital camera and video camera.

Scanner of preparations.

Image processing and storage programs in digital file.

Static telepathology.

Standards for image transfer and associated information.

g) Application of quality management systems in the laboratory:

Quality, quality management system and quality assurance: phases and circuits. Traceability.

Quality standards in the laboratory: ISO standards and GLP regulations.

Quality documents.

Certification and accreditation of the laboratory.

Audit and quality assessment.

3. Professional module: Molecular and cytogenetic biology.

Code: 1369.

Contents:

a) Characterization of the processes performed in the cytogenetic and molecular biology laboratories:

Organization and functions of the cytogenetics and cell culture laboratory.

Basic materials and equipment.

Organization and functions of the molecular biology laboratory.

Basic materials and equipment.

Sterile material handling rules. Aseptic technique.

Safety in cytogenetic and molecular biology laboratories. Disposal of hazardous waste.

Efficient use of resources.

b) Realization of cell cultures:

Cell culture types in cytogenetics: Amniotic fluid, corial vellosity, and peripheral blood. Cell types. Culture media.

Crop procurement, maintenance, and propagation techniques.

Determination of cell number and viability.

Pollution in cell culture.

c) Application of chromosomal analysis techniques:

A technique for obtaining chromosomal extensions. Cell culture and cull.

Methods of staining and chromosomal banked: patterns of identification.

Cytogenetic nomenclature.

Automation of cytogenetic analysis.

Chromosomal alterations: Numeric and structural.

prenatal diagnosis: Methods and applications.

Cytogenetics and Cancer.

d) Application of Nucleic Acid Extraction Techniques:

Structural and functional characteristics of nucleic acids.

Physical properties related to molecular biology techniques: density, denaturation, absorbance, renaturalization kinetics, and hybridization.

Restriction endonucleases and other enzymes associated with nucleic acids.

Mutations and polymorphisms.

DNA extraction techniques in peripheral blood, biopsies and tissues.

Extracting RNA.

Automatic nucleic acid extraction systems.

e) Application of PCR and electrophoresis techniques to the study of nucleic acids:

PCR techniques and variants: multiplex PCR, RT-PCR, nested PCR and real-time PCR.

Gel Electrophoresis Techniques.

Fragment display techniques and results interpretation.

Diagnostic and forensic applications of PCR techniques.

f) Application of probe hybridization techniques:

Probe types and markup types.

Hybridization Procedure: Phases.

Nucleic Acid Transfer and hybridization techniques in solid support: Southern and Northern blot. Microarrays.

Hybridization techniques in chromosomes and tissues:

FISH and variants.

HGC (comparative genomic hybridization).

FINCTION.

g) Determination of DNA cloning and sequencing methods:

Cloning: Components and phases of the cloning procedure.

Bioinformatics: Analysis of DNA and protein databases.

DNA sequencing methods:

Manual sequencing methods.

Automatic sequencing.

Pyrosectionation.

Other analysis performed with the sequencer:

Analysis of fragments.

MLPA (gene dose).

Application of molecular biology techniques in clinical diagnosis:

prenatal and pre-implantation diagnosis.

Diagnosis of neurodegenerative, cardiovascular and metabolic diseases.

Neoplasias. Diagnosis and prognosis.

Microbiological diagnosis.

Applications of molecular biology techniques in legal and forensic medicine.

4. Professional Module: General Physiopathology.

Code: 1370.

Contents:

a) Recognition of the structure and general organization of the human body:

Analysis of the body's hierarchical structure.

Citology.

Histology: Components, characteristics and function of tissues.

Classification of systems and apparatus of the organism.

Body Topography:

Address and position terminology.

Regions and body cavities.

b) Identification of the disease development process:

The pathological process.

Altering the function and the normal structure of the cell:

Adaptive changes.

reversible and irreversible cellular lesions.

semiology. Symptoms and signs.

Phases and evolution of the disease. Complications and incidences of the disease.

Disease clinic. Diagnosis. Prognosis. Treatment.

Disease groups.

Diagnostic procedures:

Clinical analysis.

Determination of electrical activity.

Diagnostic techniques through the image.

Cytological and pathological study.

Therapeutic resources.

Clinical Terminology.

c) Recognition of immune system disorders:

Natural and specific immunity: antigens and antibodies.

Immune system cells.

Cytokines.

Histocompatibility Antigens.

Immune system disorders:

Hypersensitivity reactions.

autoimmune diseases.

Immunologic deficiency syndromes.

Active and passive immunization.

d) Identification of the characteristics of infectious diseases:

Infectious agents:

Transmission and dissemination of infectious agents.

Infectious chain.

Mechanisms of lesion of microorganisms.

The inflammatory response. Components.

Acute inflammation. Morphological patterns of acute inflammation:

Suppurative Inflammation.

Mononuclear and granulomatous inflammation.

Cytoproliferative cytoproliferative inflammation.

Necrotizing inflammation.

Chronic inflammation and scarring.

Major human infectious diseases:

Gastrointestinal infections.

Viral and bacterial respiratory infections.

Opportunistic infections.

sexually transmitted diseases.

Infectious therapeutics.

e) Identification of the tumor development process:

Classification and epidemiology of neoplasms.

Molecular cancer bases:

Oncogenes.

Cancer-suppressive genes.

Biology of tumor growth.

Carcinogens:

Chemicals.

Radiation.

Oncogenic viruses.

Tumors versus tumors. Tumor antigens. Immunosurveillance.

Local and general manifestations of tumors: tumor effects in the body.

Graduation and staging of the tumor.

Prevention, diagnosis and treatment:

"Screening" and early diagnosis.

Diagnostic tests.

Therapeutic possibilities.

Most common malignancies.

f) Recognition of disease manifestations:

Respiratory physiopathology:

Respiratory Physiology.

Respiratory apparatus diseases. Respiratory failure.

Acid-base balance disorders.

Cardiocirculatory diseases:

Cardiocirculatory Physiology.

Heart and vascular manifestations. Heart failure.

Neurological diseases and the organs of the senses.

Neurological physiology and the organs of the senses.

Neurological manifestations and the organs of the senses.

Digestive tract disorders:

Digestive physiology.

Digestive, hepatic, bile, and pancreatic pathology.

Renal and urinary tract pathology:

The process of urine formation.

Renal and urinary tract pathology. Renal impairment.

g) Recognition of hemodynamic and vascular disorders:

Hemostasis and coagulation:

Normal hemostasis.

Cascade of coagulation.

thrombus and emboli formation.

Arterial and venous thrombosis.

Physiopathology of edema.

Repercussions of the irrigation blockade. Infarction:

Inchild classes.

Factors that influence the occurrence of a heart attack.

Pathology related to blood flow disorders:

Ischemic heart disease.

Pulmonary thromboembolism.

Strokes.

Arterial hypertension.

h) Recognition of endocrine-metabolic disorders and eating and metabolism:

Food and nutrition.

Hormones. More frequent endocrine disorders.

Power Physiopathology:

Nutritional, vitamin and mineral deficits.

Obesity.

Physiopathology of glucose metabolism:

Metabolism and hormone regulation of glucose.

Pathology of carbohydrate metabolism.

Diabetes. Hypoglycaemia.

Diagnostic tests.

Alterations in lipid metabolism:

Lipoproteins.

Metabolism and transport of lipids.

Atherogenesis.

Dyslipemias.

Playback physiopathology:

Hormonal regulation of reproduction.

Pathology of reproduction.

Diagnostic tests.

5. Professional Module: Necropsias.

Code: 1379.

Contents:

a) Realization of the autopsy preparation process:

Partial and total clinical autopsy.

Medical-legal autopsy.

Autopsy by multiple punctures.

Fetal autopsy.

Ecopsia.

Neuropathological.

Virtopsia.

Endodsia and radiopsia.

Organization and protocol of the preparation process.

Legislation and documentation of autopsies, ecopsies, neropathological and virtopsies:

Legislation related to medical-legal autopsies.

Legislation related to clinical autopsies.

The report.

The autopsies room. Material and media:

Clinical and medical autopsies rooms.

Mortuary and cold chambers.

Macroscopic study room of surgical pieces.

Equipment, instruments and autopsies material: applications, handling and maintenance.

Safety rules and procedures in the autopsies, ecopsies, and virtopsies room.

Pre-opening of the corpse or other activity.

Terminology, specific records, and media.

Application regulations.

b) Realization of the autopsy procedure:

Study and tasks prior to the opening of the corpse.

Neck and cervical block dissection.

Log opening techniques.

Extracting chest and abdominal organs.

Opening and study of the cranial cavity and nervous system.

Dissection and study of abdominal organs.

Taking samples in dissected, eviscerated organs and structures.

Taking samples in organs and structures using ecopsia.

Latest studies on the corpse and recomposition.

Descriptive generalities and artifacts from the macroscopic study:

Weights, measurements, color, odor, consistency and anomalies of surfaces, cavities, organs, and viscera.

Descriptive vocabulary.

Artifacts. Laboratory metastases.

Risk prevention:

Safety rules and procedures in the autopsy room.

Handling of piercing and cutting material.

Application regulations.

c) Realization of the anatomical macroscopic identification of the pattern of normality:

The macroscopic anatomy of the external autopsy examination. General characteristics. Signs of death.

Macroscopic anatomy of the skull and nervous system. Block extraction of the central nervous system. Study of the base of the skull. Pituitary. Eyeballs.

Macroscopic anatomy of the neck block.

Macroscopic anatomy of organs, vessels, and other thoracic components. Heart and big glasses.

Macroscopic anatomy of organs, vessels, and other abdominal components.

Macroscopic anatomy of organs of non-eviscerated components. Spinal cord. Bone sections.

d) Realization of the anatomic macroscopic identification of the abnormality pattern:

The macroscopic pathological anatomy of the external autopsy examination.

Injuries. Burns. Scars. Mutilations. Strange bodies.

Macroscopic pathological anatomy of the skull and nervous system.

Macroscopic pathological anatomy of the neck block.

Macroscopic pathological anatomy of organs, vessels, and other thoracic components.

Macroscopic pathological anatomy of organs, vessels, and other abdominal components.

Macroscopic pathological anatomy of non-eviscerated component organs.

e) Realization of the removal of tissues, prostheses, pacemakers, and other devices from the carcass:

Documentary protocol: Community, state and regional regulations.

Tissue sample extraction. Tissue extraction technique for DNA analysis.

Ocular balloon nucleation. Techniques for enucleation. Blefarostat.

Extracting pacemakers. Techniques of localization, dissection, electrode cutting and waterproof suture.

Extraction of osteosynthesis, stent, exoprosthesis, intramedular nails, contaminant material, or other elements. Nail disarticulation techniques for extraction.

Risk prevention.

6. Professional Module: Cytological and tissue processing.

Code: 1380.

Contents:

a) Performing the processing of the sample:

Materials, reagents, and equipment in histology and cytotechnology.

Efficient use of resources.

Safety and risk prevention in the laboratory. Waste management:

Identifying the risks associated with the techniques.

Determining prevention measures.

Waste types and disposal procedures.

Macroscopic features of the sample.

Tissue fixation process:

Fundamentals and objectives.

Processes prior to the display of the sample.

Fixer types and application rules.

Tissue decalcification and softening.

Artifacts.

Macroscopic description and sample carving.

Record and preservation of samples.

b) Realization of tissue blocks:

Fundamentals and process of inclusion of samples for optical and electron microscopy: Dehydration, clearance, and infiltration.

Preparing and making blocks. Orientation of the sample.

Preparing, scheduling, cleaning and maintaining equipment and materials.

Other processing techniques and histocytological study. Image analysis. Stereology. Autorradiography. Laser microdissection.

c) Application of cutting techniques:

Types of microtomes and components: oscillation, rotation, sliding, cryostat, and ultramicromo, among others.

Preparation of equipment. Orientation of the block and the blade.

Cutting technique according to the microbe and the composition of the block:

Paraffin Blocks.

Frozen material.

Resins for electron microscopy.

Problems in the specimen section and resolution of them.

Sample Extension and Mount:

Cuts Extension Maneuvers.

Capture and drying of preparations.

Acceding solutions for slides.

Compliance with security rules.

d) Application of staining techniques:

Fundamentals and general coloring mechanism.

Set histological colations:

Preparing the fabric.

Rationale, solvents, and protocols of the techniques.

Assembly and Conservation.

Tintorial features.

Rating of results.

Non-histochemical colouring techniques for the identification of substances: lipids, glycogen, mucin and fibrin and connective tissue, among others. Methods for neurohistological studies.

Tintions for the visualization of microorganisms.

Contrast in electron microscopy.

e) Application of histochemical and enzyme histochemical techniques:

Histochemical staining techniques. Fundamentals. Controls. Tintorial features.

Types of histochemical staining: carbohydrates, proteins and nucleic acids, lipids and pigments and metal ions.

Fundamentals, controls, and applications of enzymatic histochemistry techniques.

Enzyme classification. Sample processing for the conservation of enzymatic activity.

Taining techniques for the determination of enzymes: hydrolases, carboxylic esterases and oxidoreductases.

Histochemistry of lectins and applications. Function. Detection methods and controls.

f) Application of immunohistochemical techniques:

Monoclonal and polyclonal antibodies. Marking of the antibodies.

Fundamentals of immunohistochemical methods: direct and indirect.

Classification of techniques based on the marker used:

Immunofluorescence.

Immunoenzymatic.

Colloidal gold.

Histological processing and restoration of tissue immunoreactivity:

Antigenic recovery techniques.

Blocking endogenous enzyme activity.

Blocking background staining.

Controls.

Types of antibodies and dilutions.

Procedures for immunohistochemical techniques and controls:

Peroxidase. Signal amplifiers.

alkaline phosphatase.

Colloidal gold.

Tumor Markers:

Histochemicals.

immunohistochemicals.

Ultrastructural markers.

g) Cell Sample Processing:

Basic materials and equipment for cytological processing.

General processing of the cytological material. Extension. Fixing. Types of fixers.

Basis, reagents, and protocols of different staining techniques.

Quality control of the preparation. Preservation and archiving.

Cellular Blocks. Concept, foundation and preparation.

7. Professional Module: Gynecological Citology.

Code: 1381.

Contents:

a) Recognition of the morphological and functional characteristics of the female genital apparatus:

anatomical characteristics of the genital organs.

Histology of the female reproductive system: Histology of the uterus and cervix. Endocervix and exocervix.

Menstrual Cycle:

Ovarian cycle and endometrial cycle.

Pituitary and ovarian hormones.

Puberty and climaterio: Hormonal activity and physiological changes. Menopause.

Pregnancy and breast-feeding: Stop and breast-feeding. Genital histology in pregnancy and lactation.

Contraception.

b) Identification of the clinical data of the application for the cytological study:

Methods of gynecological exploration: vaginal and colposcopy exploration. Ultrasound. Endoscopies. Biopsies and punctures.

Types of gynecological samples:

cervical-vaginal extension. Triple sampling technique.

Liquid Cytology.

Simple vaginal cytomology. Endometrial cytology. Vulvar cytology.

Symptoms and signs in female genital pathology.

Inflammatory and infectious pathology:

Cervicovaginitis. Sexually transmitted diseases (STDs). Pelvic inflammatory disease.

Benign tumor pathology of the uterus:

Cervical and endometrial polyps. Uterine fibroids.

Malignant tumor of the uterus:

Human papillomavirus (HPV) infection.

Cervix cancer. Squamous carcinoma. Adenocarcinoma.

endometrial cancer.

Epidemiology of female and female genital cancer.

c) Analysis of gynaecological cytologies:

Cytological study techniques.

Processing, staining, and diagnostic techniques.

Technology resources in cytodiagnostics:

Automation.

Liquid citology. Cytology in monolayer.

Suitability of the sample/adequacy of the smear:

Identification. Clinical information.

Correct technique: Extension, fixation, and staining.

Cellularity.

Types of samples according to the degree of suitability.

Hormone assessment.

Physiological hormonal patterns. Cytology of hormonal alterations.

Normal cytology of female genital apparatus. Epithelial cells.

Squamous Epithelium. Endocervical epithelium. Endometrial cells.

Artifacts and contaminants in cervical-vaginal cytology.

d) Analysis of cervical-vaginal extensions in non-tumor benign pathology:

inflammatory cells.

Bacterial infections.

fungal infections.

Clamydays infections.

Virus infections.

Parasitosis.

Inflammatory changes in epithelial cells:

Changes in squamous cells.

Changes in the endocervial epithelium.

DIU Citology.

Benign reactive processes of epithelium: squamous metaplasia.

Repair changes: benign atipies.

Hyperkeratosis and paraqueratosis.

Effects of chemotherapy and radiation therapy.

e) Analysis of cervical-vaginal extensions in neoplastic processes:

Morphological alterations of HPV infection. Histological lesions and cytology.

preneoplasic lesions. Classifications:

Bethesda system.

Relationships between the different classifications.

Abnormalities of squamous epithelial cells:

Atypical squamous cells of uncertain meaning (ASCUS).

Nuclear and cytoplasmatic changes.

Scaly Intraepithelial Lesion (SIL). Histology.

Cytological characteristics of low grade SIL and high grade SIL.

Infiltrating squamous carcinoma. Histological types.

Abnormalities of glandular epithelial cells: atypical glandular cells of uncertain significance (AGUS). Nuclear and cytoplasmic changes. Endocervical adenocarcinoma.

f) Analysis of images of breast cytologies:

Breast Histology: Anatomic Characteristics of the Mom. Tissues and cells.

Scan methods.

Normal patterns in breast cytology.

Non-tumour cytopathology of the breast. Inflammations. Fibrocystic disease. Fat necrosis.

Tumor tumor of the breast: benign, malignant. Immunocytochemistry in breast cancer.

g) Analysis of cytological samples of vulva, endometrium, tubes and ovary:

Vulvar Cytology:

Normal cytology.

Inflammatory and infectious processes.

Vulvar districts.

Malignant tumors.

endometrial cytology:

Normal cytology.

Inflammatory processes. Endometritis.

endometrial hyperplasia.

Endometrial Adenocarcinoma.

Uterine tube cytomology.

Ovarian cytomology.

8. Professional Module: General Citology.

Code: 1382.

Contents:

a) Analysis of cytological imaging of the respiratory system:

Respiratory apparatus histology: respiratory tract. Lung.

Sample scan and get methods:

Brushing. Bronchoaspiration (BAS). Bronchoalveolar lavage (BAL).

Sputum. Puncture.

Normal patterns in respiratory system cytology. Respiratory tract. Lung.

Benign non-tumor cytopathology.

Tumor cytopathology: benign and malignant lesions. Immunocytochemistry in lung cancer.

b) Analysis of cytological images of lymph nodes, spleen, thymus, skin, soft tissues, and bone:

Lymph nodes and thymus:

Histology.

Scan methods.

Normal patterns in cytology.

Benign cytopathology.

Malignant cytopathology.

Skin, soft tissue, and bone:

Histology.

Scan methods.

Normal patterns in cytology.

Non-tumour cytopathology.

Tumor cytopathology of the skin, soft tissue, and bone.

c) Analysis of cytological imaging of thyroid, parathyroid, prostate, and testicle:

Thyroid and parathyroid:

Thyroid and parathyroid histology.

Scan methods.

Normal patterns in cytology.

Non-tumour cytopathology.

Inflammations.

Leisure.

Thyroid and parathyroid tumor cytopathology: benign and malignant.

Prostate and testicle:

Histology of the prostate.

Scan methods.

Normal patterns in cytology.

Prostate non-tumor cytopathology and testicle.

Tumor cytopathology of the prostate and testicle.

d) Analysis of cytological images of the digestive system:

Histology of the digestive system.

Scan methods.

Normal patterns in cytology of the oral cavity and salivary glands. Non-tumour cytopathology of the oral cavity and salivary glands. Tumor cytopathology of the oral cavity and salivary glands.

Normal patterns in esophagus and stomach cytology. Non-tumor cytopathology of the esophagus and stomach. Tumor cytopathology of the esophagus and stomach.

Normal patterns in bowel cytology.

Non-tumour cytopathology of the intestine. Tumour cytopathology of the intestine.

Normal patterns in liver and bile cytology. Non-tumor cytopathology of liver and bile. Tumor cytopathology of liver and bile.

Normal patterns in pancreatic cytology. Non-tumour cytopathology of the pancreas. Pancreatic tumor cytopathology.

e) Analysis of cytological imaging of urinary tract and adrenal glands:

Kidney:

Histology.

Scan methods.

Normal patterns in kidney cytology.

Non-tumour cytopathology.

Tumor cytopathology.

Urinary tract:

Normal patterns of urinary tract cytology.

Urinary tract non-tumor cytopathology.

Infections.

Inflammations.

Urinary tumor cytopathology: benign, malignant.

adrenal gland:

Normal patterns in adrenal gland cytology.

Adrenal non-tumor cytopathology.

Infections.

Inflammations.

Tumor cytopathology.

f) Analysis of cytological images of organic liquids, nervous system and eyeball:

Liquids and spills:

Characteristics of liquids and spills.

Ascitic liquid.

pleural liquid.

cerebrospinal fluid.

Pericardial fluid.

Synovial liquid.

Scan methods.

Normal patterns in cytology of ascitic fluid.

Cytopathology non-tumor of the ascitic fluid.

Tumor cytopathology of ascitic fluid.

Normal patterns in pleural fluid cytology.

Non-tumor cytopathology of pleural fluid.

Pleural liquid tumor cytopathology.

Normal patterns and cytopathology of cerebrospinal fluid.

Normal patterns and cytopathology of pericardial fluid.

Normal patterns and cytopathology of synovial spills.

Amniotic fluid.

Central nervous system and eyeball.

9. Professional Module: Pathological and Cytodiagnostic Anatomy Project.

Code: 1383.

Contents:

a) Identification of the needs of the productive sector and the organization of the company:

Identification of job roles.

Industry structure and organization.

Company activity and its location in the industry.

Organization chart of the company. Functional relationship between departments.

Industry trends: productive, economic, organizational, employment and other.

Work procedures in the company scope. Systems and methods of work.

Determination of excluded labor relations and special labor relations.

Collective agreement applicable to the professional field.

Company culture: corporate image.

Quality and security systems applicable in the industry.

b) Design of projects related to the sector:

Analysis of the local reality, the business offer of the sector in the area and the context in which the professional training module will be developed in the workplace.

Collecting information.

The overall structure of a project.

Crafting a work script.

Project execution planning: objectives, content, resources, methodology, activities, timing, and evaluation.

Project Feasibility and Opportunity.

Review of applicable regulations.

c) Planning for project execution:

Sequencing of activities.

Elaboration of work instructions.

Making a risk prevention plan.

Documentation required for project execution schedule.

Compliance with safety and environmental standards.

Project quality assurance indicators.

d) Defining control and evaluation procedures for project execution:

Proposal for solutions to the objectives outlined in the project and justification of the selected ones.

Defining the project evaluation procedure.

Determining the variables that can be evaluated.

Documentation required for project evaluation.

Process and end product quality control.

Log of results.

10. Professional module: Training and employment orientation.

Code: 1384.

Contents:

a) Active job search:

Valuation of the importance of permanent training for the career and professional career of the senior technician in Pathological and Cytodiagnostic Anatomy.

Analysis of personal interests, skills and motivations for the professional career.

Identification of training itineraries related to the top technician in Pathological and Cytodiagnostic Anatomy.

Responsible for learning itself. Knowledge of the requirements and expected fruits.

Definition and analysis of the professional sector of the title of Superior Technician in Pathological and Cytodiagnostic Anatomy.

Planning your own career:

Setting work goals, in the medium and long term, compatible with needs and preferences.

Realistic and consistent goals with current and projected training.

Job search process in small, mid-sized, and large companies in the industry.

Learning and employment opportunities in Europe. Europass, Ploteus.

Job search techniques and instruments.

Self-employment assessment as an alternative for professional insertion.

The decision-making process.

Setting a personal checklist of consistency between career plan, training, and aspirations.

b) Conflict management and work teams:

Valuation of the advantages and drawbacks of the team work for the organization's effectiveness.

Equipment classes in the pathological anatomy sector according to the functions they perform.

Analysis of the training of work teams.

Features of an effective work team.

The participation in the work team. Analysis of the possible roles of their members.

Conflict definition: Features, sources, and stages of the conflict.

Methods for conflict resolution or suppression: mediation, reconciliation, and arbitration.

c) Job Contract:

The right of the job.

Intervention of public authorities in industrial relations.

Analysis of the individual labor relationship.

Determination of excluded labor relations and special labor relations.

Hiring contract modes and promotion measures.

Rights and duties arising from the employment relationship.

Working Conditions. Salary, work time and work rest.

Modifying, suspending, and extinguishing the work contract.

Representation of workers and workers.

Collective bargaining as a means of reconciling the interests of the business and business staff.

Analysis of a collective agreement applicable to the professional scope of the superior technician in Pathological and Cytodiagnostic Anatomy.

Collective conflicts of work.

New work organization environments: subcontracting and teleworking, among others.

Benefits for workers and workers in new organizations: flexibility and social benefits, among others.

d) Social Security, Employment and Unemployment:

The Social Security System as a basic principle of social solidarity.

Structure of the Social Security system.

Determination of the principal obligations of the business and its staff in the field of Social Security: membership, high, low and contribution.

The protective action of Social Security.

Classes, requirements, and benefits.

Concept and situations that are protected by unemployment.

Systems of advice to the company's staff regarding their rights and duties.

e) Professional risk assessment:

Importance of preventive culture at all stages of professional activity.

Assessment of the relationship between work and health.

Analysis and determination of working conditions.

The concept of professional risk. Risk factor analysis.

Risk assessment in the company as a basic element of preventive activity.

Risk analysis linked to security conditions.

Risk analysis linked to environmental conditions.

Risk analysis linked to ergonomic and psychosocial conditions.

Specific risks in the pathological anatomy sector.

Determination of the possible health damage to the worker or worker that may arise from the identified risk situations.

f) Planning for risk prevention in the enterprise:

Rights and duties in the field of occupational risk prevention.

Responsibilities in the field of occupational risk prevention.

Managing prevention in the enterprise.

Representation of workers and workers on preventive matters.

Public bodies related to the prevention of occupational risks.

Planning for prevention in the enterprise.

Emergency and evacuation plans in work environments.

Elaboration of an emergency plan in a small or medium enterprise in the sector.

g) Application of prevention and protection measures in the enterprise:

Determination of individual and collective prevention and protection measures.

Action protocol in an emergency situation.

First aid. Medical urgency. Basic concepts.

Application of first aid techniques.

Training the company's personnel in the field of emergency plans.

Monitoring the health of workers and workers.

11. Professional module: Enterprise and entrepreneurial initiative.

Code: 1385.

Contents:

a) Entrepreneurship Initiative:

Innovation and economic development. Main features of innovation in the field of pathological anatomy and cytodiagnostics (materials, technology and production organization, among others).

Entrepreneurial culture as a social need.

The entrepreneurial character.

Key factors for entrepreneurial people: initiative, creativity and training.

Collaboration between entrepreneurs.

The performance of enterprising people as employees of a company related to pathological anatomy and cytodiagnosis.

The performance of entrepreneurial people as entrepreneurs in the pathological anatomy sector.

The risk in entrepreneurial activity.

Concept of entrepreneurship. Requirements for the exercise of business activity.

Personal goals versus business goals.

Business Plan: the business idea in the field of pathological anatomy and cytodiagnostics.

Good practices of entrepreneurial culture in the activity of pathological anatomy at the local level.

b) The company and its environment:

Basic company functions.

The enterprise as a system.

The overall business environment.

Analysis of the overall environment of a company related to pathological anatomy.

The company's specific environment.

Analysis of the specific environment of a company related to pathological anatomy.

Relationships of a pathology and cytodiagnostic company or laboratory with its environment.

Relations of a company or laboratory of pathology and cytodiagnosis with the whole of society.

Company culture: corporate image.

Social responsibility.

The Social Balance.

Business ethics.

Social and ethical responsibility of companies in the pathological anatomy sector.

c) Creating and starting a company:

Company concept.

Enterprise Types.

The responsibility of the owners of the business.

Taxation in companies.

Choice of the legal form. Dimension and number of partners.

Administrative formalities for the formation of a company.

Economic Viability and financial viability of a company related to pathological anatomy.

Analysis of the sources of financing and budgeting of a company related to pathological anatomy.

Aid, grants and tax incentives for SMEs related to pathology and cytodiagnostic laboratories.

Business Plan: Choice of legal form, economic and financial feasibility study, administrative procedures and management of grants and grants.

d) Administrative function:

Concept of basic accounting and notions.

Accounting Operations: Registering an Enterprise's Economic Information.

Accounting as a true picture of the economic situation.

Analysis of accounting information.

Corporate Tax Obligations.

Requirements and deadlines for the filing of official documents.

Administrative management of a company related to pathological anatomy.

12. Professional module: Training in job centres.

Code: 1386.

Contents:

a) Identification of the structure and business organization:

Structure and business organization of the pathological anatomy sector.

Company activity and its location in the pathological anatomy sector.

Organization chart of the company. Functional relationship between departments.

The company's logistics organization. Suppliers, customers, and marketing channels.

Work procedures in the company scope. Systems and methods of work.

Human resources in the enterprise: training requirements and professional, personal and social skills associated with different jobs.

Quality system set in the job center.

The security system set in the job center.

b) Application of ethical and labour habits:

Personal Attitudes: Empathy, punctuality.

Professional Attitudes: Order, cleanliness, responsibility and security.

Attitudes to the prevention of occupational and environmental risks.

Hierarchy in the enterprise. Communication with the work team.

Documentation of professional activities: methods of classification, coding, renewal and elimination.

Recognition and application of internal company rules, work instructions, standard work procedures, and others.

c) Managing biological samples:

Interpretation of the analysis request documents in relation to the type of sample that you want to obtain.

Managing the lab's computer applications.

Managing the collection of the different types of samples.

Classification and fractionation of samples for submission to the corresponding analysis laboratories.

Select the techniques of conservation, storage, transportation, and shipment of samples.

Application of the exclusion and rejection criteria for samples that are unfit for processing and analysis.

Application of safety and risk prevention protocols in the handling of chemical and biological products, in accordance with current regulations.

d) Managing biological samples:

Identification of the type of lab material.

Application of established cleaning, disinfection and sterilization techniques in the laboratory.

Selection of Reagents.

Identification of basic equipment and laboratory instruments. Applications.

Interpretation of standard working procedures (NPT) for the use and maintenance of basic equipment and laboratory instruments.

Realization of dissolutions and dilutions of samples and reagents.

Application of substance separation procedures.

Technical assessment of the consistency and reliability of the results obtained.

Performing microscopy techniques by applying scanning and imaging tools.

Application of quality management systems in the lab.

Identification of the processes to be performed in cytogenetics and molecular biology.

e) Realization of the procedure and macroscopic identification of the autopsy, applying protocols and recognizing patterns of normality and abnormality:

Use of legal documents to perform each type of autopsy. Terminology to use.

Identification of the equipment or equipment necessary for performing each type of autopsy.

Realization of the specific process of body preparation.

Description of the anomalies, pathological signs, and artifacts, and the associated etiology.

Recognition of anatomical macroscopic characteristics according to patterns of normality and abnormality.

Specific terminology in the macroscopic description.

Application of cleaning procedures, disinfection of instruments, equipment and facilities.

Application of quality standards, prevention of occupational risks and environmental protection throughout the process.

f) Realization of cytological and tissue processing, applying treatment protocols for samples:

Preparing the material, reagents, and equipment based on the operations to be performed.

Preparing and fixing the sample.

Realization of tissue blocks. Methods of inclusion. Selection.

Application of cutting techniques according to the block, equipment, and technique material.

Application of staining techniques.

Use of histochemical, enzyme-histochemical and immunohistochemical techniques.

Identifying the errors in the process.

Application of physical, chemical and biological risk prevention and safety measures.

Application of the generated waste disposal protocol.

g) Analysis of gynaecological and general cytologies. Patterns of normality and cellular abnormality. Existing relationship:

Assessment of the suitability requirements of the sample.

Detection and marking of artifacts and contaminants in the cytopreparations.

Defining the patterns of cell normality in the cytological sample.

Identification of the characteristics of a characteristic cytopathological pattern of each infectious, inflammatory or degenerative pathology.

Description of cellular alterations in benign and malignant neoplastic processes.

Correlation of the cytopathological findings with the clinical data.

Application of appropriate medical-clinical terminology.

ANNEX II

Sequencing and weekly hourly distribution of professional modules

Top Grade Forming Cycle: Pathological and Cytodiagnostic Anatomy

	Module	Duration (hours)	First Course (h/week)	Second
				2 Quarters (h/week)	1 quarter (hours)
1367. Managing biological samples	185	6
1368. General lab techniques	175	5
1369. Molecular and cytogenetic biology	170	5
1370. General physiopathology	190	6
1384. Job training and guidance	90	3
1385. Enterprise and entrepreneurship	60	2
reserved for the English imparted module	90	3
1379. Necropsies	60		3
1380. Cytological and tissue processing	180		9
1381. Gynaecological cytology	160		8
1382. General cytology	160		8
reserved for the English language module	40
1386. Job center training	400			400
1383. Pathological and cytodiagnostic anatomy project	40			40
in the formative cycle	2,000	30	30	440

ANNEX III

Modules that are likely to be imparted in English.

1367. Management of biological samples.

1368. General laboratory techniques.

1369. Molecular biology and cytogenetics.

1370. General physiopathology.

1380. Cytological and tissue processing.

1381. Gynaecological cytology.

1382. General cytology.

ANNEX IV

Minimum spaces and equipment

Spaces:

Form Space

M²
30 pupils or students	20 pupils or students
Multi-purpose Aula	60	40
Lab molecular and pathological anatomy	100	80
cytodiagnostic technique	60	40

Minimum Equipment:

Forative Space

-purpose Aula.	Computer-installed computers, projection systems, and the Internet. Audio-visual media. Application software.
Laboratory of molecular biology and anatomy pathological.	Study. Invested Microscope. Fabric processor and storage cabinet for surgical parts formulated. Flammable Substance Safety Armarium. Refrigerator Security Armarium. Extractive Campaign. Laboratory Study (2). Microtomes (1 × 4 students or Cold plates (1 × every 2 microtomes). Thermostatic Bath. Include Baths. paraffin Station or Paraffin dispensers. Cold plates. Thermostatic plate. Cytocentrifuge. Staining equipment. Necrop_table. Fridge. Freezer. Balances, stirrers, and pH-meters. Volumetric material. Microscopes. Space for the placement of computer equipment that connect to microscopes. Digital camera for macro and microphotography. Table of playback.
Microscopes
Microscopes.