LEGE no. 43 43 of 11 April 2014 (* updated *) on protection of animals used for scientific purposes ((updated on 11 May 2015 *)
ISSUER
PARLIAMENT
---------- The Romanian Parliament adopts this law + Chapter I General provisions + Article 1 Subject matter and scope (. This law establishes measures for the protection of animals used for scientific or other experimental purposes and, for that purpose, lays down rules governing the following: ... a) replacement and reduction of the use of animals in procedures, as defined in art. 2 lit. a), as well as the improvement of methods of rearing, housing, care and use of animals in procedures; ... b) the origin, reproduction, marking, care and accommodation, and the killing of animals; ... c) activities of breeders, suppliers and users of animals; ... d) evaluation and authorization of projects involving the use of animals in procedures, as defined in art. 2 lit. b). ... ((. This law shall apply where the animals are used in procedures or intended for use in procedures or where they are specifically increased in order for their organs or tissues to be used for scientific purposes. or educational. ... (3) This law shall apply until such time as the animals referred to in paragraph 1. (2) are killed, relocated or returned to a suitable habitat or growth system. ... ((4) The elimination of pain, suffering, stress or lasting harm through the effective use of anaesthetic, analgesic or other similar methods does not preclude the use of an animal in procedures within the scope of this law. ... ((. This Law shall apply to the following animals: ... a) non-human animals vertebrate live, including larval forms that feed autonomously and foetal forms of mammals from the last third of their normal development stage; ... b) cephalopods. ... (6) This law applies to animals used in procedures and which are at a stage of development earlier than that provided in par. ((5) lit. a), if the animal is to be left alive after this stage of development and, following the procedures carried out, it is likely to present pain, suffering, stress or lasting harm after reaching that stage of development. ... ((7) This Law shall not apply to: ... a) non-experimental acts in agricultural holdings; ... b) veterinary acts practiced in clinics, for non-experimental purposes; ... c) studies carried out in veterinary clinics necessary to authorise the marketing of a veterinary medicinal product; ... d) acts performed in the framework of recognised animal husbandry ... e) acts carried out for the main purpose of animal identification; ... f) acts that are not likely to cause pain, suffering, considerable stress or lasting harm equivalent or stronger than those produced by the introduction of a needle, with good veterinary practices. ... + Article 2 Definitions For the application of this law, the terms and expressions below signify, as follows: a) the procedure-any use, invasive or non-invasive, of the animal for experimental purposes or for other scientific purposes, with known or unknown results or for educational purposes, which may cause the animal a certain level of pain, suffering, stress or injury of duration equivalent or even stronger than those caused by the introduction of a needle with good veterinary practice; any action that pursues or may result in the birth or hatching is included an animal or the creation and maintenance of a genetically modified animal line in any of the these conditions, however, the killing of animals for the sole purpose of the use of organs or their tissues ... b) project-a programme of work with defined scientific objectives, which involves the use of one or more procedures; ... c) generic multiple project-project carried out on the basis of usual methods for the purpose of testing, diagnosis or production within a single authorization, but without these methods being excluded from the project evaluation; ... d) unit-any structure, any building, any group of buildings or any other space, which may include a place that is not completely closed or covered, as well as mobile installations; ... e) breeder-any natural or legal person, for profit or not, who increases the animals set out in Annex no. 1, in order to be used in procedures or for the scientific use of tissues or organs originating from them or which increase other animals, mainly for these purposes; ... f) supplier-any natural or legal person, for profit or not, other than a breeder, who provides animals for use in procedures or for the scientific use of tissues or organs originating from ... g) user-any natural or legal person, for profit or not, who uses animals in procedures; ... h) competent authority-the National Veterinary Health and Food Safety Authority, respectively the units subordinated to it; ... i) disabling condition-reducing the normal physical or psychological functioning of a person; ... j) autonomous colony-a colony in which the animals are grown exclusively within it or come from other colonies, but without being caught from the wild, and the animals are housed in a way that ensures their familiarity with humans; ... k) inspection-activity defined according to art. 2 2 section 7 7 of Commission Implementing Regulation (EU) No 882/2004 of the European Parliament and of the Council of 29 April 2004 on official controls carried out to ensure the verification of compliance with feed and food law and animal health and animal health rules animal welfare. ... + Article 3 Principle of replacement, reduction of the use of animals in procedures and improvement of methods of farming, housing, care and use of animals in procedures (. In the place of a procedure, a scientifically satisfactory method or strategy shall be used which does not involve the use of live animals. ... (2) The number of animals used in projects must be minimised, without compromising the objectives of the project involving the use of animals in procedures. ... (3) All measures should be taken to improve the conditions for farming, housing and animal care and the methods used in the procedures, by eliminating or minimising pain, suffering, stress or the lasting harm that may be caused to animals. ... (4) The provisions of this Article shall apply with regard to the performance of the procedures, in compliance with the requirements of art. 12. ... + Article 4 Procedures for (. Procedures may be carried out only for the following purposes: ... a) basic research; ... b) transferable or applied research; ... c) for any of the purposes referred to in lett. b) in the development, production and testing of the quality, efficacy and safety of medicinal products, food and feed and other substances or products; ... d) protection of the natural environment in the interest of health or welfare of humans or animals ... e) research for the conservation of species; ... f) higher education or professional training for the acquisition, maintenance or improvement of professional skills; ... g) forensic investigations. ... (2) The transferable or applied research referred to in par. ((1) lit. b) the following aims: ... a) prevention, prophylaxis, diagnosis or treatment of diseases, poor health states or other abnormalities or their effects in humans, animals or plants; ... b) evaluation, detection, control or modification of physiological diseases in humans, animals or plants; ... c) animal welfare and improvement of production conditions for farmed animals. ... + Article 5 Methods of killing ((1) The killing of animals must be carried out only by means of methods which cause the lowest level of pain, suffering or distress. ... ((2) Animals must be killed in the establishment of a breeder, supplier or user, by a competent person, trained and prepared in this regard, who meets the conditions laid down in art. 22. ... (3) In the case of a practical application in the field, the animal may be killed by the competent person according to para. ((2), outside a unit. ... ((. As regards the animals referred to in Annex no. 4 4, the appropriate killing method indicated in that Annex shall be used. ... (. The competent authority may grant, at the request of the applicant, derogations from the requirements of paragraph 1. (4), in the following situations: ... a) to allow the use of another method, provided that the method is considered at least as human on the basis of scientific evidence; ... b) where, on the basis of a scientific justification drawn up by the applicant, it is demonstrated that the purpose of the procedure cannot be achieved by using a method of killing set out in Annex no 4. ... (6) Provisions of para. ((2)-(4) shall not apply where an animal is to be killed in cases of emergency, for reasons of animal welfare, public health, animal health or the environment. The attestation of these situations must be carried out by the veterinarian designated according to the 24. ... + Chapter II Provisions concerning the use of certain animals in + Article 6 Endangered species ((1) Examples of the endangered species listed in Annex A to Commission Implementing Regulation (EU) No 338/97 of the Council of 9 December 1996 on the protection of species of wild fauna and flora by regulating trade therein, which does not fall within the scope of 7 7 para. (1) of that Regulation shall be used only in procedures which satisfy the following conditions: ... a) the procedure has one of the aims provided in art. 4 4 para. ((1) lit. c) or e) or art. 4 4 para. ((2) lit. a); ... b) there is a scientific justification, drawn up by the applicant, that the purpose of the procedure cannot be achieved by the use of species other than those listed in that Annex. ... (2) Provisions of para. ((1) shall not apply to non-human primate species. ... + Article 7 Nonhuman primate ((. Examples of non-human primates shall not be used in procedures unless the procedures meet the following cumulative conditions: ... a) the procedure has one of the aims provided in art. 4 4 para. ((1) lit. c) or art. 4 4 para. ((2) lit. a) and is carried out in order to avoid, prevent, diagnose and treat potentially deadly or disabling conditions that occur in humans or in art. 4 4 para. ((1) lit. a) or c); ... b) there is a scientific justification, according to which the purpose of the procedure cannot be achieved by the use of species other than non-human primates. ... ((2) Examples of non-human primates listed in Annex A to Commission Implementing Regulation (EU) No 338/97 ,, which does not fall under art. 7 7 para. (1) of that Regulation shall not be used in proceedings unless the procedures meet the following cumulative conditions: ... a) the procedure has one of the aims provided in art. 4 4 para. ((1) lit. c) or art. 4 4 para. ((2) lit. a) and is carried out in order to avoid, prevent, diagnose and treat potentially deadly or disabling conditions that occur in humans or in art. 4 4 para. ((1) lit. e); ... b) there is a scientific justification that the purpose of the procedure cannot be achieved by the use of species other than non-human primates and the use of species not included in Annex A to Commission Implementing Regulation (EU) No 338/97 . ... (3) In compliance with the provisions of par. ((1) and (2), large monkeys shall not be used in procedures, subject to the use of the protection clause provided for in art. 49 49 para. ((2). ... + Article 8 Animals caught from the wild ((. Animals caught from the wild shall not be used in procedures. ... (. The competent authority may grant derogations from the prohibition referred to in paragraph 1. (1) on the basis of a scientific justification, whereby the applicant demonstrates that the purpose of a procedure cannot be achieved by the use of an increased animal for procedures. ... (3) The capture of animals in the wild shall be made by competent persons, authorized to carry out this activity under the law, and by methods that do not cause animals pain, suffering, stress or lasting harm that can be avoided. ... (4) Conduct of activities targeting species of wild animals strictly protected under the art. 33 of Government Emergency Ordinance no. 57/2007 on the regime of protected natural areas, the conservation of natural habitats, flora and wildlife, approved with amendments and additions by Law no. 49/2011 , as amended, shall be subject to the derogations established according to that emergency ordinance. ... (5) If, after capture or during it, it is found that the animal is injured or in poor health, it is examined by a veterinarian or another person who meets the requirements laid down in art. 24 24 and measures shall be taken to minimise the suffering of the animal and, where there is a scientific justification, the competent authority may grant derogations from the requirement to take measures to minimise the suffering of the animal. ... (6) The procedure for granting the derogations provided in par. (5) shall be established by joint order of the President of the National Veterinary Health and Food Safety Authority, the Minister of the Environment and Climate Change and the Minister of Health, within 90 days of entry into force of the present law. ... + Article 9 Animals raised for use in procedures ((. Animals belonging to the species listed in Annex no. 1 1 may be used in proceedings only if they have been raised for this purpose. ... (2) Non-human primates set out in Annex no. 2 2 may be used in procedures, from the date set out in that Annex, only if they are followed by non-human primates grown in captivity or originating from autonomous colonies. ... ((. On the basis of a scientific justification, the competent authority may grant derogations from the conditions imposed by paragraph 1. ((1) and (2), based on the procedure provided for in art. 8 8 para. ((6). ... + Article 10 Stray and semi-wild animals of domestic species (. The use in procedures of stray and semi-wild animals of domestic species shall be prohibited. ... (. The competent authority may grant exemptions from the provisions of paragraph 1. ((1) only in cases where the following cumulative conditions are met: ... a) there is an essential need for studies on the health and welfare of those animals or serious threats to human or animal health or the environment; ... b) there is a scientific justification, according to which the purpose of the procedure can only be achieved by using a stray or undomesticated animal. ... + Chapter III Procedures + Article 11 Conditions for carrying out procedures ((. Procedures shall be carried out only within the user's establishment. ... (2) Any derogation from paragraph ((1) is granted only on the basis of a scientific justification. ... ((3) Procedures shall be carried out only in a project. ... + Article 12 Choosing methods (. Without prejudice to the provisions of national legislation prohibiting certain types of methods, a procedure shall be carried out only if there is no other method or strategy for testing accredited at European Union level to achieve the result intended and not involving the use of live animals. ... (. Where the procedures are chosen, those who are most likely to provide satisfactory results and meet the following requirements shall be selected: ... a) use a minimum number of animals; ... b) involve animals with the lowest capacity to feel pain, suffering, stress or to present lasting harm; ... c) causes the lowest level of pain, suffering, stress or lasting harm. ... ((. Death as the end point of a procedure shall be avoided to the extent possible and shall be replaced by early and human endpoints. If death cannot be avoided as a final point, the procedure shall be so designed that: ... a) lead to the death of a small number of animals; and ... b) reduce to a minimum possible the duration and intensity of the animal's suffering and, as far as possible, ensure a painless death. ... + Article 13 Anesthesia (1) Except for cases where this is inappropriate, the procedures are performed under general or local anesthesia or use the painkillers or another appropriate method to ensure that pain, suffering or stress are reduced to minimum level. Procedures that can cause serious injuries that can cause severe pain are not performed without anesthesia. ... ((. When deciding on the appropriateness of using anaesthesia, the following criteria shall be taken into account: ... a) if the anesthesia is considered to be more traumatic for the animal than the procedure itself; ... b) if anesthesia is incompatible with the purpose of the procedure. ... (3) The animal administration of a medicinal product to stop or restrict the manifestation of pain without an adequate degree of anesthesia or analgesia shall be prohibited. In these cases, a scientific justification shall be provided, accompanied by details of the anesthesia or analgesia scheme. ... (4) An animal that may suffer from pain once the effect of anaesthesia disappears is treated with preventive and postoperative painkillers or other appropriate palliative methods, provided that this is compatible with the purpose of the procedure. ... ((. As soon as the purpose of the procedure has been achieved, the necessary measures shall be taken to minimise the suffering of the animal. ... + Article 14 Classification of the severity of procedures ((. Procedures shall be classified in one of the categories of severity set out in Annex no. 7 7, respectively "without recovery", superficial "," moderate "or" severe " according to each individual case, using the award criteria set out in that Annex. ... (2) Except for the situation provided in art. 49 49 para. ((3), it is prohibited to carry out a procedure if it involves severe pain, suffering or stress that may last for a long time and which cannot be improved. ... + Article 15 Reuse of animals in procedures (. An animal which has been used in one or more procedures may be reused in a new procedure in favour of an animal which has not been used in the procedures only if the following conditions are met: ... a) the actual severity of the previous proceedings was superficial or moderate; ... b) it is demonstrated that the general state of health and well-being of the animal is completely restored; ... c) the subsequent procedure is classified as "superficial", "moderate" or "without recovery"; ... d) the veterinary indications are observed, taking into account the life experience of the animal. ... (2) By exception to the provisions of par. ((1) lit. a) and following a veterinary examination of the animal, the competent authority may permit the reuse of an animal, provided that the animal has only been used once in a procedure that has assumed severe pain, stress or Equivalent suffering. ... + Article 16 Final procedure (1) A procedure is considered to be completed if there are no other comments to be made in relation to that procedure or, in the case of new modified genetic lines of the animal, where no more or more expected reactions of pain, suffering, stress or lasting harm to the offspring, of an equivalent intensity, or superior to that produced by the introduction of a needle. ... (2) At the end of a procedure, the veterinarian or, as the case may be, another competent person, according to the law, in this field makes a decision on keeping the animal alive. The animal is killed in a situation where it is possible to remain with pain, suffering or moderate or severe stress or lasting harm. ... (3) If the animal is kept alive, it shall receive care and shelter appropriate to its health condition. ... + Article 17 Joint use of organs and tissues (1) The National Veterinary Health and Food Safety Authority shall facilitate, where appropriate, the establishment of programmes for the joint use of organs and tissues of the animals killed. ... (2) The procedure for the establishment of programs of joint use of organs and tissues from the animals killed shall be established by joint order of the President of the National Veterinary Health and Food Safety Authority, of the Minister environment and climate change and the Minister of Health, within 90 days of the entry into force of this Law. ... + Article 18 Release and relocation of animals The competent authority may allow animals used in procedures or intended for use in procedures to be relocated or returned to their habitat or to a breeding system appropriate to that species if the following are fulfilled. conditions: a) the health of the animal allows it; ... b) there is no danger to public health, animal health or the environment; ... c) all appropriate measures have been taken to protect the welfare of the animal. ... + Chapter IV Authorisation + Article 19 Veterinary authorisation of breeders, suppliers and users of animals (. All breeders, suppliers and users of animals must be authorized veterinary veterinary medicinal products by the competent authority. The authorization shall be granted for a period of 5 years. ... (2) The veterinary authorisation procedure is established by Order of the President of the National Veterinary Health and Food Safety Authority no. 16/2010 for the approval of the Veterinary Health Rule on the procedure for the registration/veterinary authorization of the establishments/collection centres/holdings of origin and means of transport in the field of animal health and welfare, the units involved in the storage and neutralisation of animal by-products not intended for human consumption and processed products, as amended and supplemented. ... (. The veterinary authorization shall be granted only if the breeder, supplier or user of the animals and its establishment complies with the requirements laid down in this Chapter. ... (4) The veterinary authorization shall specify the person responsible for ensuring compliance with the provisions of this law, as well as the person or persons referred to in art. 23 23 para. ((1) and in art. 24. ... (5) The renewal of the veterinary authorization is necessary in the event of any significant change in the structure or operation of the unit of a breeder, supplier or user who could adversely affect the welfare of the animals. ... (6) Under the conditions provided in par. ((4), the veterinary authorization shall be renewed for a period of 5 years. ... (7) Replacing the person or persons referred to in par. ((3) the competent authority shall be notified to the competent authority within a maximum of 15 days from the date of their replacement. ... + Article 20 Suspension and withdrawal of authorisation (. Where a breeder, supplier or user of animals no longer meets the requirements laid down in this Chapter, the competent authority shall, by expert staff, apply the main contravention sanctions provided for by the Government Decision no. 984/2005 on the establishment and sanctioning of contraventions to veterinary health and food safety rules, with subsequent amendments and completions, accompanied, as the case may be, by the complementary contravention sanction of suspension or withdrawal veterinary authorization. ... ((. In the case of suspension or withdrawal of the veterinary authorization by the competent authority, the welfare of the animals housed in the establishment shall not be adversely affected. ... + Article 21 Requirements for installations and equipment (1) All establishments belonging to a breeder, supplier or user of animals must possess suitable facilities and equipment for both the sheltered animal species and the performance of the procedures, where they are performed. ... (2) The model, construction and operation mode of the installations and equipment provided in par. (1) must ensure that procedures are carried out in a more efficient manner and are intended to achieve reliable results using a minimum number of animals and causing a minimum degree of pain, suffering, distress or lasting harm. ... (3) In order to apply the provisions of para. (the competent authority shall take the necessary measures to comply with the requirements set out in Annex no. 3. ... + Article 22 Staff competence (. Each breeder, supplier and user of animals shall have sufficient staff. ... (2) The staff provided in par. ((1) has the necessary education and training before performing any of the following activities: ... a) carrying out procedures on animals; ... b) design of procedures and projects involving the use of animals in procedures; ... c) animal care; ... d) killing animals. ... (3) Persons carrying out the activities referred to in par. ((2) lit. b) must be licensed in a scientific discipline relevant to the work carried out and have specific knowledge of the species. ... (4) The staff performing the activities referred to in par. ((2) lit. a), c) or d) must be supervised in the performance of his duties, until he demonstrates that he has the necessary competence. ... (. The competent authority shall ensure, by veterinary authorization, that the requirements laid down in paragraph 1 are met. ((2)-(4). ... (6) Based on the elements set out in Annex no. 5 are established, by order of the President of the National Veterinary Health and Food Safety Authority, the minimum requirements on education and professional training and the requirements for obtaining, maintaining and demonstrating competence necessary for the activities provided in par. ((2). ... + Article 23 Specific staff requirements (. Each breeder, supplier and user of animals shall have one or more persons in the establishment, who shall: ... a) answer/respond to the supervision of the welfare and care of the animals ... b) instruct/instruct the personnel in charge of the animals, so that it holds the specific information on the species housed within the establishment; ... c) is/are responsible/responsible for ensuring that the staff has the appropriate education, competence and professional training and that it is supervised until it proves the necessary competence. ... (2) Persons referred to in art. 39 39 para. ((2) lit. b) must take all measures because: ... a) any unnecessary pain, suffering, distress or injury that is caused to animals during a procedure to be stopped; ... b) projects involving the use of animals in procedures to be carried out with the project authorization, and, in case of non-compliance with the conditions of authorization, the appropriate measures to rectify it be taken and recorded. ... + Article 24 Designated veterinarian Every breeder, supplier and user of animals used for scientific or educational purposes shall have a veterinarian designated by the competent authority, with experience in the medicine of animals used for scientific purposes or educational or expert with an appropriate qualification, where necessary, in charge of animal welfare advice and treatment. + Article 25 Structure responsible for animal welfare (. Each breeder, supplier and user of animals used for scientific purposes or other experimental purposes shall establish a structure responsible for animal welfare. ... ((. The animal welfare structure shall comprise at least the person or persons responsible for animal welfare and care and, in the case of a user, a member of scientific competence. The structure responsible for animal welfare also benefits from the contribution of the designated veterinarian or the expert referred to in art. 24. ... (. The competent authority may allow small breeders, suppliers and users of animals to perform by other means the tasks referred to in art. 26 26 para. ((1). ... + Article 26 Tasks of the animal welfare structure (1) The structure responsible for animal welfare, provided for in art. 25, must meet at least the following tasks: ... a) advise staff dealing with animals on animal welfare issues during the purchase, shelter, care and use of animals; ... b) advise staff on the application of the requirement to replace, reduce and improve the use of animals in procedures and to inform them of technical and scientific progress on the application of that requirement; ... c) establish and assess the internal operational processes for monitoring, reporting and subsequent actions necessary for the welfare of animals housed within the establishment; ... d) follow the developments and results of the projects taking into account the effect on the animals used and identifying the elements contributing even more to the replacement, reduction and improvement of the methods of growth, housing, care and use of animals in procedures; ... e) to provide advice for resettlement programmes, including on the proper socialisation of animals to be relocated. ... ((. All records of the recommendations made by the animal welfare structure and of the decisions taken with regard to those recommendations shall be kept for a period of at least 3 years. ... (3) The records provided in par. (2) must be made available to the competent authority at its request. ... + Article 27 Growth strategy of non-human primates Non-human primate breeders must develop and apply a strategy for increasing the percentage of animals that are followed by non-human primates grown in captivity. + Article 28 Resettlement or animal release programme Where the competent authority allows the relocation of animals, breeders, suppliers and users from which the animals leave in question are required to establish a resettlement scheme aimed at the socialisation of those animals; in the case of Wild animals shall establish a rehabilitation programme, as appropriate, before they are released into their habitat. + Article 29 Animal records (. All breeders, suppliers and users of animals shall keep records, which shall contain at least the following data: ... a) the number and species of animals grown, purchased, supplied, used in procedures, issued or relocated; ... b) the origin of the animals, including whether they have been increased for use in procedures ... c) the dates on which the animals were purchased, supplied, issued or relocated; ... d) from whom the animals were obtained; ... e) the name and address of the ... f) the number and species of animals that have died or have been killed in each establishment; for dead animals the cause of death is recorded, if known; ... g) in the case of users, projects in which animals are used. ... (2) Registers provided in par. ((1) shall be kept for a minimum of 5 years and shall, upon request, be made available to the competent authority. ... + Article 30 Information on non-human dogs, cats and primates (1) All breeders, suppliers and users of non-human dogs, cats and primates must keep records of the following information on each dog, cat and non-human primate: ... a) identity; ... b) place and date of birth, if they are available; ... c) if increased for use in procedures; ... d) if it is a follower of captive-bred non-human primates, in the case of non-human primates. ... (2) Each dog, cat and non-human primate must have an individual file, accompanying the animal, as long as it is increased under the conditions of this law. ... (3) The individual file referred to in par. ((2) it shall be drawn up at the birth of the animal or as soon as possible after it and shall contain any relevant reproductive, veterinary and social information on that animal, as well as on the projects in which it was used. ... (. The information provided for in this Article shall be kept for a minimum of 3 years after the animal's death or relocation and, upon request, shall be made available to the competent authority ... (5) In case of relocation of animals, the individual file provided in par. ((2) accompany the animal. ... + Article 31 Marking and identification of dogs, cats and non-human primates (. Each dog, cat or non-human primate shall be identified at the latest at the time of weaning with a permanent individual mark in the least painful manner possible. ... (2) If a dog, a cat or a non-human primate is transferred from a breeder, supplier or user to another, before being weaned and, for practical reasons, cannot be marked in advance, the recipient shall, until marked the animal, a register in which the identity of the animal's mother is mainly stated. ... ((3) If a dog, cat or unmarked unhuman primate, which is weaned, is received by a breeder, supplier or user, it shall be permanently marked as soon as possible and in the least painful manner possible. ... (. The manufacturer, the supplier and the user must submit, at the request of the competent authority, the necessary justifications showing the reasons why the animal is not marked. ... + Article 32 Care and shelter of animals (1) All animals must benefit from shelter, environment, food, water and proper care for their health and well-being. ... ((2) Restrictions on the extent to which an animal can satisfy its physiological and ethological needs must be limited to the essentials. ... (3) The physical conditions in which the animals are grown, kept or used must be checked daily. ... ((4) Any deficiency or pain, suffering, stress or lasting injury that may be avoided must be eliminated as soon as possible. ... (5) Animals must be transported under appropriate conditions. ... (6) The standards of care and accommodation provided for in Annex no. 3 3 shall apply from 1 January 2017. ... (7) The competent authority may grant derogations from paragraph 1. (1) and (6) for scientific reasons, animal welfare or animal health. ... (8) The procedure for granting the derogations provided in par. (7) is established by joint order of the President of the National Veterinary Health and Food Safety Authority, the Minister of the Environment and Climate Change and the Minister of Health, within 90 days of entry into force of the present law. ... + Article 33 Inspections carried out by the competent authority ((1) The inspections shall be carried out periodically to all breeders, suppliers and users of animals, including their establishments, in order to verify compliance with the measures imposed on them by the provisions of this law. ... (. The competent authority shall adapt the frequency of inspections on the basis of a risk analysis for each establishment, taking into account: ... a) the number and species of animals housed; ... b) the history of the breeder, supplier or user regarding compliance with the provisions of this law; ... c) the number and types of projects carried out by the respective user; ... d) any information that could indicate non-compliance with the provisions of this ... ((3) The inspections shall be carried out annually, in at least one third of the animal users, with the risk analysis provided in par. ((2). Breeders, suppliers and users of non-human primates are inspected at least once a year. ... (. The competent authority shall carry out inspections without prior notification, where there are reasonable grounds for concern over the infrastructure and the functioning of the national controls, and the correct application of the categories of severity. ... ((. The records of inspections carried out shall be kept for at least 5 years. ... + Article 34 Inspection of inspections by the European Commission ((1) Where the European Commission carries out controls on the infrastructure and the way of carrying out national inspections by the competent authority, and on the correct classification of the procedures in the projects in the categories provided in art. 14, the competent authority shall provide all necessary assistance to the experts of the European Commission in the ... (. The competent authority shall take all necessary measures to ensure that the results of the checks referred to ((1) be taken into account. ... + Article 35 Project authorisation ((. Projects involving the use of animals in proceedings shall be carried out only with a prior authorisation from the competent authority and with it. ... ((2) The projects shall be carried out only after having received, in advance, a favorable evaluation from the competent authority, according to the provisions of art. 37. ... + Article 36 Application for project authorisation (. The user of the animals or person responsible for the project must submit an application for the authorization of the project to the competent authority. ... (. The application shall include at least the following elements: ... a) project proposal; ... b) a non-technical summary of the project; ... c) information on the elements set out in Annex no. 6. ... + Article 37 Project evaluation (. A degree of detail shall be applied to the evaluation of the projects, and shall be verified if the projects meet the following criteria: ... a) the project is scientifically or educationally justified or required according to the law; ... b) the aims of the project ... c) the project is designed so as to allow the procedures to be carried out in the most humane manner possible and in compliance with the environment. ... ((. The evaluation of projects involving the use of animals in procedures shall consist in particular of: ... a) an evaluation of the objectives of the project, of possible scientific benefits or of its educational value; ... b) an assessment of the conformity of the project with the requirements for the replacement, reduction and improvement of the use of ... c) an assessment of the classification of the severity of the procedures and the attribution ... d) a damage-benefit analysis of the project, to assess whether the harm caused to animals in terms of suffering, pain and stress is justified by the expected result, taking into account ethical considerations and whether it could ultimately be in benefit of human, animal or environmental; ... e) an evaluation of any justification provided for in art. 5-11 5-11, art. 13 13, 15 and 32; ... f) specifying the circumstances and when the project should be evaluated retrospectively. ... ((. When assessing projects, the competent authority carrying out the evaluation of the projects shall take into account, in particular, the expertise in the following areas ... a) the areas of scientific activity for which animals are used, including the replacement, reduction and improvement of the use of animals in procedures in those areas ... b) the design of procedures, including statistics, where necessary; ... c) veterinary practice in the science of laboratory animals or, where appropriate, veterinary practice in wildlife science; ... d) the breeding and care of animals of the species to be used ... (4) The project evaluation process should be transparent. Subject to the protection of intellectual property rights and confidential information, the evaluation of the projects shall be carried out in an impartial manner. ... + Article 38 Review of (1) In cases where this is specified, with the provisions of art. 37 37 para. ((2) lit. f), the retrospective evaluation shall be carried out by the competent authority which, on the basis of the necessary documentation submitted by the user, ... a) if the project objectives have been met; ... b) the harm caused to animals, including the number and species of animals used, and the severity of the procedures; ... c) any elements that could contribute to strengthening the application of the requirement for replacement, reduction and improvement of the use of animals in procedures ... ((2) All projects using non-human primates and projects involving procedures classified as severe, including the procedures provided for in art. 14 14 para. ((2), are subject to a retrospective assessment. ... (3) Without prejudice to the provisions of par. (2) and by exception to art. 37 37 para. ((2) lit. f), the competent authority may exempt from the obligation of retrospective assessment projects which include only procedures classified as moderate or without recovery. ... + Article 39 Granting of the project authorisation ((. The authorisation of the project involving the use of animals in procedures by the competent authority shall be limited to the procedures which have been subject to: ... a) a project evaluation; ... b) the degree of severity allocated to those procedures. ... (. The project authorization shall specify the following: ... a) the user conducting the project; ... b) the persons responsible for the implementation of the project as a whole and in compliance with the conditions in which the project was authorized; ... c) the units in which the project will take place, in the event that is the case; ... d) any specific conditions imposed as a result of the evaluation of the project, including whether the project needs to be evaluated retrospectively and the date on which it must be evaluated retrospectively. ... (3) The project authorization shall be granted for a maximum period of 5 years. ... (4) The competent authority may authorise multiple generic projects carried out by the same user, if these projects meet the provisions of this law or if they use animals for production or diagnostic purposes with tested methods. ... + Article 40 The period within which the project authorisation is granted and communicated (1) The project authorization shall be granted and communicated by the competent authority to the applicant no later than 40 working days after receipt of the complete and correct application, formulated according to the provisions of art. 36 36 para. ((2). This term also includes the project evaluation. ... (2) If the project is complex or multidisciplinary, the competent authority may extend the term provided in par. ((1) only once, with an additional period not exceeding 15 working days. The extension of the term, as well as the duration of this extension shall be duly substantiated and shall be notified to the applicant before the expiry of the ((1). ... (3) The competent authority shall confirm to the applicant the receipt of all requests for authorization within 15 days and shall indicate the period referred to in ((1) in which the project authorization is to be granted. ... ((. In the event of an incomplete or incorrect application, the competent authority shall inform the applicant within 15 days of the need to submit additional documents and of the possible consequences for the applicable time limit. ... + Article 41 Simplified administrative procedure (1) The National Veterinary Health and Food Safety Authority shall ensure, at national level, the promotion of the simplified administrative procedure for the authorisation of projects containing procedures classified as "without recovery", "superficial" or "moderate" times that do not use non-human primates, which are necessary to comply with normative requirements or that use animals for production or diagnostic purposes using established methods. ... (. Where the simplified administrative procedure referred to in paragraph 1 applies. (1), the following requirements shall be met: ... a) the application shall specify the elements referred to in art. 39 39 para. ((2) lit. a)-c); ... b) an evaluation of the project is carried out, in accordance with art. 37 37; ... c) the term referred to in art. 40 40 para. ((1). ... ((3) The rules on the authorization of projects through the simplified administrative procedure, provided in par. (1), shall be approved by joint order of the President of the National Veterinary Health and Food Safety Authority, the Minister of National Education and the Minister of Health, within 90 days of the entry into force of this Law. ... (4) The joint order of the President of the National Veterinary Health and Food Safety Authority, the Minister of National Education and the Minister of Health, provided in par. ((3), provides for the duties and obligations of all issuing institutions. ... + Article 42 Non-technical summaries of projects involving the use of animals in procedures ((1) Subject to the protection of intellectual property rights and confidential information, the non-technical summary of the project shall present: ... a) information on the objectives of the project, including the harm to animals and the expected benefits, number and types of animals to be used; ... b) a demonstration of the conformity of the project with the requirements for the replacement, reduction and improvement of the use of animals ... (2) The non-technical summary of the project is anonymous and does not contain names and addresses of users and their staff. ... (. The competent authority may require the non-technical summary of the draft to indicate whether it is to be subject to retrospective evaluation and the time limit for it. In this case, the competent authority shall ensure, through checks carried out by the specialist staff, that the non-technical summary of the project is updated according to the results of the retrospective assessment. ... (4) The competent authority shall publish on its own website the non-technical summaries of the authorized projects, as well as all their updates. ... + Article 43 Renewal and withdrawal of the project authorisation (1) Any significant modification of the project which would cause a negative effect on the welfare of the animal shall be notified to the competent authority within 30 days and shall require the granting of a new project authorisation, subject to a favorable outcome of the project evaluation, with the provisions of this law ... ((2) If it finds that the project is not carried out according to the project authorization, the competent authority may withdraw this authorization. ... ((. In the case of withdrawal of the project authorisation, the welfare of the animals used or intended for use in the project shall not be affected ... + Article 44 Project documentation (. The entire relevant documentation, including project authorisations and the outcome of the project evaluation, shall be kept for at least 3 years from the date of expiry of the project authorisation and shall be made available to the competent authority upon request. ... (2) By exception to the provisions of par. ((1), the documentation of projects requiring retrospective evaluation shall be kept until the retrospective evaluation is completed. ... + Chapter V Avoiding duplication of procedures and alternative methods + Article 45 Avoiding duplication of procedures Data from another Member State of the European Union that are resulting from procedures recognised by the European Union legislation shall be accepted, if no further procedures are required for that data, for reasons related to the protection of health and public safety or the environment. + Article 46 Alternative methods for the use of animals (1) The National Veterinary Health and Food Safety Authority shall contribute to the development and validation of alternative methods which could ensure at least the same level of information as that obtained from the procedures carried out on animals, but which does not involve the use of animals or uses fewer animals or involving less painful procedures and undertakes any other actions it deems necessary to encourage research in this field. ... (2) The National Veterinary Health and Food Safety Authority shall assist the European Commission in identifying and nominating appropriate specialized and qualified laboratories to carry out validation studies of alternative methods provided in par. (1), carried out within the Joint Research Centre of the Commission. ... (3) The National Veterinary Health and Food Safety Authority shall ensure, at national level, the promotion of alternative methods to the use of animals in procedures and the dissemination of information on them. ... (4) The National Veterinary Health and Food Safety Authority shall advise on the relevance and suitability of the alternative methods proposed for validation. ... + Article 47 National Committee for the protection of animals used for scientific or educational purposes (1) The National Veterinary Health and Food Safety Authority shall organise and operate the National Committee for the Protection of Animals used for scientific purposes which provide the structures responsible for protection animals of the competent authority advice on issues related to the procurement, growth, housing, care and use of animals in procedures and participate in the exchange of information on best practices in this regard. ... (2) The national committee referred to in par. ((1) consists of representatives of the academic environment in the biomedical field, of the College of Veterinary Physicians, of other professional associations and of animal protection organizations, with experience in the field regulated by this law. ... (3) The composition and the organization and functioning regulations of the National Committee provided in par. (1) is approved by order of the President of the National Veterinary Health and Food Safety Authority. ... (4) The national committee referred to in par. ((1) exchange information with the national animal protection committees used for scientific and educational purposes in the other Member States of the European Union on the functioning of the structures responsible for the protection of animals, on the evaluations of projects involving the use of animals in procedures and pooling best practices within the European Union. ... (5) At the level of the specialized university centers in Romania, the local research ethics committees are organized and operated, which provide the responsible animal protection structures within the competent authority issues related to the acquisition, growth, housing, care and use of animals in procedures and participate in the exchange of information on best practices in this regard. ... + Article 48 Reporting (1) By November 10, 2018 and subsequently, every 5 years, the National Veterinary Health and Food Safety Authority shall submit to the European Commission the information on the implementation of the provisions of this Law and in particular special of art. 9 9 para. (1), of art. 25, 27, 33, 37, 38, 42 and 45. ... (2) The National Veterinary Health and Food Safety Authority collects and makes public, annually, on its own website, statistical information on the use of animals in procedures, including information on the severity of the procedures, and the origin and species of non-human primates used in procedures. Until November 10, 2015 and subsequently, annually, the National Veterinary Health and Food Safety Authority shall submit to the European Commission this statistical information, on paper and in electronic format. ... (3) The National Veterinary Health and Food Safety Authority shall submit annually to the European Commission detailed information on the derogations granted pursuant to art. 5 5 para. ((5) lit. a). ... + Article 49 Safeguard clauses (1) If the National Veterinary Health and Food Safety Authority has scientifically justifiable reasons to consider that it is essential to use non-human primates for the purposes provided in art. 7 7 para. ((1) lit. a) with regard to human beings, but this use is not undertaken to avoid, prevent, diagnose and treat potentially deadly diseases or which may induce a disabling condition, it may establish a transitional measure authorising such use, provided that its purpose cannot be achieved by the use of species other than non-human primates. ... ((2) Where there are reasonable grounds for considering that it is essential to take measures for the conservation of species or to combat an unexpected epidemic, a deadly disease or which may induce a disabling condition which occurs in humans, the National Veterinary Health and Food Safety Authority can establish a transitional measure allowing the use of large monkeys in procedures carried out in one of the purposes provided for in art. 4 4 para. ((1) lit. c) or e) or para. ((2) lit. a), provided that the purpose of the proceedings in question cannot be achieved by the use of species other than large apes or by the use of alternative methods. Reference to art. 4 4 para. ((2) lit. a) is not interpreted as referring to animals and plants. ... ((3) Where, for exceptional and scientifically justifiable reasons, the National Veterinary Health and Food Safety Authority considers that it is necessary to allow the use of a procedure that causes pain, suffering or severe stress, which tends to be long-lasting and which cannot improve, as provided in art. 14 14 para. ((2), may establish a transitional measure authorising such a procedure. The National Veterinary Health and Food Safety Authority may decide not to allow the use of non-human primates in such procedures. ... (4) If the National Veterinary Health and Food Safety Authority intends to establish a transitional measure according to the provisions of par. ((1), (2) or (3), it shall inform the European Commission and the other Member States of the European Union without delay, giving the reasons for determining the transitional measure and the evidence proving the situation of the type described in paragraph 1. ((1)-(3) underlying the transitional measure. ... (5) The transitional measure provided in par. ((1)-(3) is established by order of the President of the National Veterinary Health and Food Safety Authority and may be applied during the period provided for by decision of the European Commission or may cease at the request of the European Commission. ... + Chapter VI Transitional and final provisions + Article 50 Sanctions (1) The facts that constitute contraventions to the measures imposed by this Law, as well as the sanctions applicable to them shall be established by Government decision, within 90 days from the date of entry into force of this Law. ... (2) The National Veterinary Health and Food Safety Authority shall notify the European Commission of the provisions establishing the sanctions provided for in paragraph 1. (1), as well as any subsequent amendment or completion thereof. ... + Article 51 Transposition The National Veterinary Health and Food Safety Authority shall inform the European Commission of the normative acts and administrative measures necessary for the implementation of this Law. + Article 52 Transitional provisions (1) For projects involving the use of animals in procedures approved before the date of entry into force of this Law and whose duration does not exceed January 1, 2018, the provisions of art. 35-44. ... ((2) Projects involving the use of animals in procedures approved before the date of entry into force of this Law and whose duration extends beyond January 1, 2018 shall only be granted until this date. ... + Article 53 Annex Annexes no. 1-7 are an integral part of this law. + Article 54 Final provisions On the date of entry into force of this Law, Government Ordinance no. 37/2002 for the protection of animals used for scientific purposes or for other experimental purposes, published in the Official Gazette of Romania, Part I, no. 95 of 2 February 2002, approved with amendments by Law no. 471/2002 , as amended and supplemented, shall be repealed. * This law transposes Directive 2010 /63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes, published in the Official Journal of the European Union (JOUE) L series, no. 276 276 of 20 October 2010. This law was adopted by the Romanian Parliament, in compliance with the provisions of art. 75 75 and art. 76 76 para. (2) of the Romanian Constitution, republished. CHAMBER OF DEPUTIES PRESIDENT VALERIU-STEFAN ZGONEA SENATE PRESIDENT CĂLIN-CONSTANTIN-ANTON POPESCU-TARICEANU Bucharest, April 11, 2014. No. 43. + Annex 1 LIST animals referred to in art. 9 9 of law 1. Mouse (Mus musculus); 2. Rat (Rattus Norwegian); 3 3. Cobai (Cavia porcellus); 4. Syrian Hamster (gold) (Mesocricetus auratus); 5. Chinese Hamster (Cricetulus griseus); 6. Gerbil mongol (Meriones unguiculatus); 7. Rabbit (Oryctolagus cuniculus); 8 8. Caine (Canis familiaris); 9. Cat (Felis catus); 10. All non-human primate species; 11. Frog [Xenopus (laevis, tropicalis), Rana (temporaria, pipiens)]; 12. Zebra fish (Danio rerio). + Annex 2 LIST non-human primates and the date from which they may be used under art. 9 9 para. ((2) of the Act ┌ ------------------------------- -------------------------------------------------------------- | Species | Date from which can be used in procedures | ├ ------------------------------- 留言 | 加入好友 | Monkeys cynomolgus | Date on which marks 5 years from | | (Macaca fascicularis) | publication of feasibility study *) | | Monkeys rhesus (Macaca mulatta) | made by the European Commission, provided that | | Other non-human primate species | study not to recommend an extended period | └ ------------------------------- -------------------------------------------------------------- _________ *) Provisions of art. 10 10 para. ((1) the fourth subparagraph of Directive 2010 /63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes, the European Commission, in consultation with the Member States of the European Union and stakeholders, shall carry out a feasibility study, including an assessment of animal welfare and health, with regard to the requirement laid down in art. 9 9 para. ((2) of the law. That study shall be published by 10 November 2017. The study shall be accompanied, where appropriate, by proposals to amend this Annex. + Annex 3 REQUIREMENTS on establishments, care and housing of animals + Section A General section 1. Installations 1.1. Functions and General Design a) All installations must be constructed in such a way as to ensure a suitable environment for the species housed in the light of their physiological and ethological needs. Installations must also be designed and managed in such a way as to prevent unauthorised persons from accessing and entering or escaping animals. ... b) Units must have an active maintenance program to avoid and remedy any deficiency of buildings or equipment. ... 1.2. Sheltering spaces a) Units must have a regular and effective program to clean rooms and maintain satisfactory hygiene standards. ... b) The walls and floors must be covered with wear-resistant materials caused by the animals and the cleaning process. The material must not harm the animal's health and must not cause injury to them. Each equipment or accessory must have additional protection so as not to be damaged by the animals or to injure the animals. ... c) Incompatible species, for example, predator and prey, or animals that require different environmental conditions must not be housed in the same space, and in the case of predatory combination and prey, animals must not be housed within the same limits visual, olfactory or auditory. ... 1.3. Spaces for general and specific procedures a) The units must have, as the case may be, laboratory facilities for carrying out simple diagnoses, post-mortem examinations and/or sampling to be subject to more in-depth laboratory examinations elsewhere. There must be spaces for general and specific procedures, for situations where it is not desirable for the procedures and observations to be carried out in the accommodation spaces. ... b) For the isolation of newly-arrived animals special spaces must be made available until their health is established and until the potential health risk for the other animals is assessed and reduced to a minimum. ... c) Separate spaces must be provided for housing sick or injured animals. ... 1.4. Service spaces a) Storage spaces are designed, used and maintained so as to preserve the quality of food and bedding. As far as possible, these spaces should not contain parasites or insects. Materials that may be contaminated or present danger to animals or personnel must be stored separately. ... b) The cleaning and washing spaces must be sufficiently roomy for the installations necessary for the decontamination and cleaning of the equipment used. The cleaning process is thus carried out to separate the flow of the material clean from that of the dirty material, to prevent contamination of the freshly cleaned equipment. ... c) Units ensure the safe storage and disposal of animals and animal waste. ... d) Where it is necessary to perform surgical procedures in aseptic conditions there should be one or more properly equipped rooms and spaces intended for post-operative recovery. ... 2. Environment and its control 2.1. Ventilation and temperature a) Isolation, heating and ventilation of the sheltering space guarantee that air circulation, dust level and gas concentration are kept within limits that are not harmful to the sheltered animals. ... b) The temperature and relative humidity in the housing spaces must be adapted to the species and age groups housed. Temperature should be measured and recorded daily. ... c) Animals must not be isolated in outdoor spaces under climatic conditions that may harm them. ... 2.2. Lighting a) If the natural light does not provide an appropriate light-dark cycle, a controlled illumination shall be provided to meet the biological needs of the animals and to ensure a satisfactory working environment. ... b) Enlightenment must meet the needs for animal breeding and control. ... c) It is mandatory to ensure regular periods of light and an intensity of light adapted to species. ... d) If albino animals are housed, account should be taken of their sensitivity to light. ... 2.3. Noise a) Noise levels, including ultrasound, must not adversely affect animal welfare. ... b) The units must have alarm systems that emit sounds outside the spectrum of sound perception of animals, if this does not prevent the perception of those sounds by humans. ... c) Sheltering spaces must, where appropriate, have sound insulation and noise-absorbing materials. ... 2.4. Alarm systems a) Units that depend on electrical or mechanical equipment for environmental control and protection must have a backup system to ensure the functioning of essential services, emergency lighting systems and systems of Alarm. ... b) Heating and ventilation systems must be equipped with surveillance devices and alarms. ... c) It is mandatory to display clearly clear instructions on procedures in case of emergency. ... 3. Animal care 3.1. Health a) A strategy must be implemented in establishments to ensure that an appropriate state of health is maintained which guarantees the welfare of animals and complies with scientific requirements. This strategy must include regular animal surveillance, a microbiological surveillance programme and action plans for health problems and must define health control parameters and procedures in the case of the introduction of new animals. ... b) Animals shall be subject to inspection by a competent person at least every day. These checks ensure that all sick or injured animals are identified and appropriate measures are taken. ... 3.2. Animals caught from the wild a) Transport containers and means of transport adapted to those species must be available at the catching sites where the animals must be moved for examination or treatment. ... b) Special attention must be paid to and appropriate measures must be taken to acclimate, quarantine, shelter, increase, care for animals caught from the wild and, where appropriate, provision for their release end of proceedings. ... 3.3. Sheltering and enriching the living environment a) Admission ... Animals, except those that are naturally solitary, must be housed in stable groups of compatible individuals. In cases where individual accommodation is permitted in accordance with art. 32 of the law, duration must be limited to the minimum period necessary and must maintain visual, auditory, olfactory and/or tactile contact with the other animals. The introduction or reintroduction of animals into stable groups must be carefully supervised so as to avoid problems of incompatibility and disruption of social relations. b) Enrichment of the living environment ... All animals must have a space with sufficient complexity, allowing the manifestation of a wide range of normal behaviors. Animals must benefit from a certain degree of control over their environment and a certain freedom of choice, in order to reduce stress-induced behaviour. The units must have life-enriching techniques that broaden the range of activities available to animals and enhance their adaptation activities, including physical exercise, food search, activities. Appropriate handling and cognitive species. Enrichment of the living environment in animal enclosures must adapt to the needs of the species as well as the individual needs of those animals. The strategies for enriching the living environment in units must be reviewed and updated periodically. c) Animal enclosures ... Animal enclosures must not be made of materials likely to harm animal health. Their design and construction must be carried out so as not to hurt the animals Unless they are disposable, the enclosures must be built from materials that resist cleaning and decontamination techniques. The design of the floors in the animal enclosures must be adapted to the species and age of the animals and they must be designed to facilitate the removal of the manure. 3.4. Feeding a) The form, content and presentation of the diet must comply with the nutritional and behavioral needs of the animal. ... b) Feed must be tasty and uncontaminated. When selecting the raw materials, namely the production and presentation of food, the units must take measures to minimise chemical, physical and microbiological contamination. ... c) Packaging, transport and storage must be carried out in such a way as to avoid contamination, damage or destruction. All food sheds, containers and other utensils used to feed the animals must be cleaned periodically and, if necessary, sterilised. ... d) All animals must have access to food and have sufficient space to limit competition with other animals. ... 3.5. Watering a) Animals must always benefit from uncontaminated drinking water. ... b) If automatic watering systems are used, they must be checked periodically, must be regularly maintained and cleaned to avoid accidents. Where solid bottom cages are used, particular attention should be paid to the limitation of the risk of flooding. ... c) It is necessary to adapt the water supply of aquariums and basins to the needs and tolerance limits of individual fish species, amphibians and reptiles. ... 3.6. Rest and sleeping areas a) Animals must be permanently disposed of for bedding or sleeping structures adapted to species, including nests or structures for breeding animals. ... b) Inside animal enclosures, with the needs of species, must ensure a solid and comfortable rest area for all animals. All sleeping areas must be kept clean and dry. ... 3.7. Handling The units shall establish appropriate accommodation and training programmes for the animals, procedures and duration of the project. + Section B Species-specific section 1. Mice, rats, gerbils, hamsters and guinea pigs In this table and in all the following tables relating to mice, rats, gerbils, hamsters and guinea pigs, "enclosure height" means the vertical distance between the enclosure floor and the enclosure roof and this height applies to over 50% of the the minimum floor area of the enclosure before the addition of the life-enrichment devices. When drawing up the procedures, the potential increase of the animals should be taken into account, so as to ensure adequate space (as detailed in Tables 1.1-1.5) throughout the duration of the study. Table 1.1 Mice *Font 8 * ┌ ---------------------------- [...] [...] [...] [...] [...] [...] | | Body weight | Surface | Surface | Height | Date to which it is | | | (g) | minimum a | podelei | minimum a | make reference | | | | | | enclosure | per animal| enclosure | in art. 32 32 | | | | (cmp) | (cmp) | (cm) | para. ((6) of | | | | | | | | law | ├ ---------------------------- [...] [...] [...] [...] [...] | Normal accommodation and during | up to 20 inclusive | 330 | 60 | 12 | 1 January 2017 | | proceedings | | | | | | | | | over 20 and up | 330 | 70 | 12 | | | | | | at 25 inclusive | | | | | | | | | | | | | | | | | over 25 and up | 330 | 80 | 12 | | | | | | at 30 inclusive | | | | | | | | | | | | | | | | | over 30 | 330 | 100 | 12 | | | | ├ ---------------------------- [...] [...] [...] [...] [...] | Reproduction | | 330 | | 12 | | | | | | | | | | | | | | | | | | | | | | | | | | | monogama | | | | | | | | | (related | | | | | | | | | | | or not) | | | | | | | | | | | | | | | | | | | | trio (in-| | | | | | | | rudite). | | | | | | | | | | | | | | | | | | | | | | | | | | | femela | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | add | | | | | | | 180 cmp | | | | | | ├ ---------------------------- [...] [...] [...] [...] [...] | Normal accommodation at breeders | under 20 | 950 | 40 | 12 | | | | | (*) | | | | | | | | | Size of enclosure 950 cmp | | | | | | | ├ ---------------------------- [...] [...] [...] [...] [...] | Enclosure size | under 20 | 1,500 | 30 | 12 | | | | | 1,500 cmp | | | | | | | | └ ---------------------------- [...] [...] [...] [...] [...] [...] ___________ (*) The weaned mice may be kept under the conditions of these higher stocking densities, for a short period after weaning until dispatch, provided that the animals are housed in larger spaces that benefit from adequate enrichment. of the living environment and that these housing conditions do not cause any welfare deficiency, such as an increased level of aggression, morbidity or mortality, stereotypes and other behavioural impairments, weight loss or other physiological or behavioral stress reactions. Table 1.2 Rats *Font 8 * ┌ ---------------------------] [...] [...] [...] [...] [...] [...] | | Body weight | Minimum surface | Surface | Height | Date to which it is | | | (g) | of enclosure | podelei | minimum a | make reference | | | | (cmp) | per animal| enclosure | in art. 32 32 | | | | | (cmp) | (cm) | para. ((6) of | | | | | | | | law | ├ --------------------------- ------------------------------------ 留言 | 加入好友 ---- | Normal accommodation and in | up to 200 inclusive | 800 | 200 | 18 | 1 January 2017 | | time of proceedings (*) | | | | | | | | | over 200 and | | | | | | | | | up to 300 inclusive | 800 | 250 | 18 | | | | | | | | | | | | | over 300 and | | | | | | | | | up to 400 inclusive | 800 | 350 | 18 | | | | | | | | | | | | | over 400 and | | | | | | | | | up to 600 inclusive | 800 | 450 | 18 | | | | | | | | | | | | | over 600 | 1,500 | 600 | 18 | | | ├ --------------------------- ------------------------------------ 留言 | 加入好友 ---- | Reproduction | | 800 | | 18 | | | | | | | Mother and her pups | | | | | | | | For each | | | | | | | | | adult animal | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | 400 cmp | | | | | | ├ --------------------------- ------------------------------------ 留言 | 加入好友 ---- | Normal accommodation at | up to 50 inclusive | 1,500 | 100 | 18 | | | | breeders (**) | | | | | | | | | Enclosure size | over 50 and | | | | | | | 1,500 cmp | up to 100 inclusive | 1,500 | 125 | 18 | | | | | | | | | | | | | over 100 and | | | | | | | | | up to 150 inclusive | 1,500 | 150 | 18 | | | | | | | | | | | | | over 150 and | | | | | | | | | up to 200 inclusive | 1,500 | 175 | 18 | | | ├ --------------------------- ------------------------------------ 留言 | 加入好友 ---- | Normal accommodation at | up to 100 inclusive | 2,500 | 100 | 18 | | | | breeders (**) | | | | | | | | | Target size | over 100 and | | | | | | | | | 2,500 cmp | up to 150 inclusive | 2,500 | 125 | 18 | | | | | | | | | | | | | over 150 and | | | | | | | | | up to 200 inclusive | 2,500 | 150 | 18 | | | └ ---------------------------] [...] [...] [...] [...] [...] [...] _________ ((*) In the case of long-term studies, where the dimensions of the individual spaces available to animals fall below the values indicated above towards the end of such studies, priority should be given to maintaining social structures stable. (**) The weaned rats can be kept under these higher stocking densities, for a short period after weaning until dispatch, provided that the animals are housed in larger spaces that benefit from an enrichment. appropriate to the living environment and that these housing conditions do not cause any welfare deficiency, such as an increased level of aggression, morbidity or mortality, stereotypes and other behavioural impairments, weight loss or other physiological or behavioural stress reactions. Table 1.3 Gerbil *Font 8 * ┌ ---------------------------] [...] [...] [...] [...] [...] [...] | | Body weight | Minimum surface | Surface | Height | Date to which it is | | | (g) | of enclosure | podelei | minimum a | make reference | | | | (cmp) | per animal| enclosure | in art. 32 32 | | | | | (cmp) | (cm) | para. ((6) of | | | | | | | | law | ├ --------------------------- ------------------------------------ 留言 | 加入好友 ---- | Accommodation normal and in timpuls| up to 40 inclusive | 1,200 | 150 | 18 | 1 January 2017 | | procedures | over 40 | 1,200 | 250 | 18 | | | ├ --------------------------- ------------------------------------ 留言 | 加入好友 ---- | Reproduction | | 1,200 | | 18 | | | | | | | Pereche | | | | | | | | | monogama or | | | | | | | | | trio with chicken | | | | | | └ ---------------------------] [...] [...] [...] [...] [...] [...] Table 1.4 Hamsters *Font 8 * ┌ ---------------------------] [...] [...] [...] [...] [...] [...] | | Body weight | Minimum surface | Surface | Height | Date to which it is | | | (g) | of enclosure | podelei | minimum a | make reference | | | | (cmp) | per animal| enclosure | in art. 32 32 | | | | | (cmp) | (cm) | para. ((6) of | | | | | | | | law | ├ --------------------------- ------------------------------------ 留言 | 加入好友 ---- | Accommodation normal and in timpul| up to 60 inclusive | 800 | 150 | 14 | 1 January 2017 | | proceedings | | | | | | | | | over 60 and | | | | | | | | | up to 100 inclusive | 800 | 200 | 14 | | | | | | | | | | | | | over 100 | 800 | 250 | 14 | | | ├ --------------------------- ------------------------------------ 留言 | 加入好友 ---- | Reproduction | | 800 | | 14 | | | | | | Mother or | | | | | | | | | | | | | | | | | | monogamous with chicken | | | | | | ├ --------------------------- ------------------------------------ 留言 | 加入好友 ---- | Normal accommodation at | | | | | | | | | breeders (*) | Under 60 | 1,500 | 100 | 14 | | | └ ---------------------------] [...] [...] [...] [...] [...] [...] _________ (*) The weaned hamsters may be kept under the conditions of these higher stocking densities, for a short period after weaning until dispatch, provided that the animals are housed in larger spaces that benefit from adequate enrichment. of the living environment and that these housing conditions do not cause any welfare deficiency, such as an increased level of aggression, morbidity or mortality, stereotypes and other behavioural impairments, weight loss or other physiological or behavioral stress reactions. Table 1.5 Cobai *Font 9 * ┌ -------------- [...] [...] [...] [...] [...] [...] | | Body weight | Minimum surface | Surface | Height | Date to which it is | | | (g) | of enclosure | podelei | minimum a | make reference | | | | (cmp) | per animal| enclosure | in art. 32 32 | | | | | (cmp) | (cm) | para. ((6) of | | | | | | | | law | ├ -------------- 留言 | 加入好友 ---- [...] [...] | Normal accommodation | up to 200 inclusive | 1,800 | 200 | 23 | 1 January 2017 | | and during | over 200 and up | 1.800 | 350 | 23 | | | | | procedures | at 300 including | | | | | | | | over 300 and up to | 1,800 | 500 | 23 | | | | | 450 including | | | | | | | | | over 450 and up to | 2,500 | 700 | 23 | | | | | 700 inclusive | | | | | | | | | over 700 | 2,500 | 900 | 23 | | | ├ -------------- 留言 | 加入好友 ---- [...] | Reproduction | | 2,500 | | 23 | | | | | | | Pair with chicken | | | | | | | | | For each | | | | | | | | | female | | | | | | | | | | | breeding | | | | | | | | | | additional | | | | | | | | | | add 1,000 cmp | | | | | | └ -------------- [...] [...] [...] [...] [...] [...] [...] ---------- Table 1.5 of Annex 3 was amended by art. unique from LAW no. 96 96 of 7 May 2015 , published in MONITORUL OFFICIAL no. 316 316 of 8 May 2015. 2. Iepuri During agricultural research, where the purpose of the research requires animals to be housed under conditions similar to housing animal housing conditions, animal housing must meet at least the standards laid down. by the Veterinary Health Regulation on the protection of farmed animals, approved by Order of the President of the National Veterinary Health and Food Safety Authority no. 75/2005 . Within the enclosure there must be a higher surface. This surface must allow the animals to lie down, sit and move easily underneath and must not cover more than 40% of the floor area. If, for scientific or veterinary reasons, an elevated area cannot be used, the enclosure must be 33% higher for a rabbit alone and 60% higher for 2 rabbits. If a higher surface is provided for rabbits less than 10 weeks of age, the size of the higher surface shall be at least 55 x 25 cm and the height of the floor surface shall be such that the animals be able to use all its surface. Table 2.1 Rabbits over 10 weeks of age Table 2.1 is valid for both cages and pens. The additional floor area is at least 3,000 cmp for each rabbit for the third, fourth, fifth and sixth rabbits and must be added a minimum of 2,500 cmp for each additional rabbit where there is more than one rabbit. 6 6 rabbits. *Font 9 * ┌ ---------------------------] [...] [...] [...] [...] | Final body weight | Minimum floor area | Height | Date mentioned | | (kg) | for one or two | minimum | at art. 32 32 para. ((6) | | | harmonious animals from | (cm) | from the law | | | social point of view | | | | | | (cmp) | | | | ├ --------------------------- ------------------------------------------ 留言 | 加入好友 ----- | under 3 | 3,500 | 45 | January 1, 2017 | | | | | | | | between 3 and 5 inclusive | 4.200 | 45 | | | | | | | | | | over 5 | 5.400 | 60 | | | └ ---------------------------] [...] [...] [...] [...] Table 2.2 Female with chicken *Font 9 * ┌ ---------------------------] [...] [...] [...] [...] [...] | Female weight | Minimum surface | Supplement | Height | Date provided | | (kg) | of the enclosure | for | minimum | at art. 32 32 | | | (cmp) | nests | (cm) | para. ((6) of | | | | fatare | | law | | | | (cmp) | | | | ├ --------------------------- 留言 | 加入好友 ---- -- | under 3 | 3,500 | 1,000 | 45 | January 1, 2017 | | | | | | | | | between 3 and 5 inclusive | 4.200 | 1,200 | 45 | | | | | | | | | | | over 5 | 5.400 | 1.400 | 60 | | | | | | | | | | └ ---------------------------] [...] [...] [...] [...] [...] Table 2.3 Rabbits less than 10 weeks of age Table 2.3 is valid for both cages and pens. *Font 9 * ┌ ---------------------------] [...] [...] [...] [...] [...] | Age | Minimum surface | Surface | Height | Date provided | | | of the enclosure | minimum a | minimum | in art. 32 32 | | | (cmp) | podelei/ | (cm) | para. ((6) | | | | animal | | from the law | | | | (cmp) | | | | ├ --------------------------- 留言 | 加入好友 ---- -- | From weaning | 4,000 | 800 | 40 | January 1, 2017 | | up to 7 weeks | | | | | | | | | | | | | | | Between 7 and 10 weeks | 4,000 | 1,200 | 40 | | | | └ ---------------------------] [...] [...] [...] [...] [...] Table 2.4 Rabbits: Optimal dimensions for the higher surfaces in the enclosures with the dimensions indicated in Table 2.1 *Font 9 * ┌ -------------------------- [...] [...] [...] [...] [...] | Age in weeks | Final weight | Size | Height | Date provided | | | of the body | optimal | optimal | in art. 32 32 | | | (kg) | (cm x cm) | from | para. ((6) | | | | | the floor | of the law | | | | | enclosure | | | | | | | (cm) | | | ├ -------------------------- 留言 | 加入好友 ---- 留言 | 加入好友 -- | Over 10 | Sub 3 | 55 x 55 | 25 | 1 January 2017 | | | | | | | | | | Between 3 and 5 | 55 x 30 | 25 | | | | | including | | | | | | | | | | | | | | Over 5 | 55 x 35 | 30 | | | └ -------------------------- [...] [...] [...] [...] [...] 3. Cats Cats should not be housed individually for more than 24 consecutive hours. Cats that are repeatedly aggressive towards other cats must be housed individually only if they are not found a compatible partner. Social stress in all individuals housed in the pair or in the group should be supervised at least weekly. Females of up to 4 weeks of age or females in the last two weeks of gestation may be housed individually. Table 3 Cats The minimum space in which a female can be held with her pups is the space for a single cat, which must be gradually increased so that, at the age of 4 months, the chicks have been relocated to adult space standards. Areas for feeding and lithiers shall not be located less than 0,5 m apart and shall not be exchanged between them. ┌ ----------------------------------- [...] [...] [...] [...] [...] | | Floor (*) | Shelves | Height | Date provided | | | (sqm) | (sqm) | (m) | in art. 32 32 | | | | | | para. ((6) | | | | | | | by law | ├ ----------------------------------- 留言 | 加入好友 ---- | Minimum surface area for an animal | | | | | | | adult | 1.5 | 0.5 | 2 | | | ├ ----------------------------------- 留言 | 加入好友 ---- | For each additional animal | | | | | | | | add | 0.75 | 0.25 | | | | | └ ----------------------------------- [...] [...] [...] [...] [...] _________ (*) Floor area without shelves. 4. Dogs Dogs must be provided, where possible, external ingradations. Dogs should not be housed individually for more than 4 consecutive hours. The inner enclosures must represent at least 50% of the minimum available dog space as detailed in Table 4.1. The space standards further detailed are based on the values required for Beagle breed dogs, but large breeds, such as Saint Bernard or the Irish greyhound, must dispose of spaces much larger than detailed ones. in Table 4.1. For breeds other than laboratory Beagle, space standards must be agreed upon in consultation with veterinary staff. Table 4.1 Dogs Dogs housed in the pair or in the group can be restricted each to half of the total available space (2 sqm for a dog under 20 kg, 4 sqm for a dog over 20 kg) in the situation where they are subject to procedures within the meaning of this law, in where this isolation is essential for scientific purposes. The period during which a dog is kept in such a limited space shall not exceed 4 consecutive hours. A lactating female and her pups must benefit from the same standards of space as a single female with an equivalent weight. The calving boxes must be designed so that the female can move into an additional box or an elevated area away from the chicken. *Font 9 * ┌ ------------- [...] [...] [...] [...] [...] [...] | Weight (kg) | Surface | Minimum surface | For each | Height | Date provided | | | minimum a | a floor for | minimum animal | minimum (m) | in art. 32 32 | | | enclosure | one or two | add a minimum | | para. ((6) | | | (sqm) | animals (sqm) | by (sqm) | | by law | ├ ------------- 留言 | 加入好友 ----- 留言 | 加入好友 ... | Up to 20 | 4 | 4 | 2 | 2 | 1 January 2017 | | including | | | | | | | | | | | | | | | | Over 20 | 8 | 8 | 4 | 2 | | | └ ------------- [...] [...] [...] [...] [...] [...] Table 4.2 Dogs-reserve after weaning *Font 9 * ┌ -------------------------- [...] [...] [...] [...] [...] | Weight of the dog (kg) | Minimum surface | Surface | Height | Date provided | | | of the enclosure | minimum a | minimum | in art. 32 32 | | | (sqm) | floor/| (m) | para. ((6) | | | | animal | | from the law | | | | (sqm) | | | | ├ -------------------------- 留言 | 加入好友 ---- 留言 | 加入好友 -- | Up to 5 inclusive | 4 | 0.5 | 2 | January 1, 2017 | ├ -------------------------- 留言 | 加入好友 ---- 留言 | 加入好友 -- | Over 5 | | | | | | | and up to 10 inclusive | 4 | 1.0 | 2 | | | ├ -------------------------- 留言 | 加入好友 ---- 留言 | 加入好友 -- | Over 10 | | | | | | | and up to 15 inclusive | 4 | 1.5 | 2 | | | ├ -------------------------- 留言 | 加入好友 ---- 留言 | 加入好友 -- | Over 15 | | | | | | | and up to 20 inclusive | 4 | 2 | 2 | | | ├ -------------------------- 留言 | 加入好友 ---- 留言 | 加入好友 -- | Over 20 | 8 | 4 | 2 | | | └ -------------------------- [...] [...] [...] [...] [...] Table 5 Ferrets *Font 9 ┌ -------------------------- [...] [...] [...] [...] [...] | | Minimum surface | Surface | Height | Date provided | | | of the enclosure | minimum a | minimum | in art. 32 32 | | | (cmp) | floor/| (cm) | para. ((6) | | | | animal | | from the law | | | | (cmp) | | | | ├ -------------------------- 留言 | 加入好友 ---- 留言 | 加入好友 -- | Animals up to 600 g | 4,500 | 1,500 | 50 | January 1, 2017 | | including over 20 | | | | | | | | | | | | | | | Animals over 600 g | 4,500 | 3,000 | 50 | | | | | | | | | | | Adult males | 6,000 | 6,000 | 50 | | | | | | | | | | | Femela and its pups | 5,400 | 5,400 | 50 | | | └ -------------------------- [...] [...] [...] [...] [...] 6. Unhuman Primate Young non-human primates should not be separated from their mothers up to 6 to 12 months of age, depending on the species. The environment must give non-human primates the opportunity to carry out a complex program of daily activities. The enclosure must allow non-human primates to adopt a behavioral repertoire as vast as possible, provide them with a sense of safety and a sufficiently complex environment to allow the animals to run, move, climb and climb. to jump. Table 6.1 Marmosets and tamarines ┌ ------------ [...] [...] [...] [...] [...] | | Minimum surface | Minimum volume | Height | Provided date | | | of the premises | for each minimum | | at art. 32 32 | | | for one (*) | animal supplementar| enclosure | para. ((6) | | | or two animals, | with age over | (m) (**) | of the law | | | plus their pups with | 5 months | | | | | | lower age | (mc) | | | | | | | by 5 months | | | | | | | | (sqm) | | | | | | ├ ------------ [...] [...] [...] [...] [...] | Marmoseti | 0.5 | 0.2 | 1.5 | January 1, 2017 | ├ ------------ 留言 | 加入好友 ----- 留言 | 加入好友 | Monkeys | | | | | | | tamarin | 1.5 | 0.2 | 1.5 | | | └ ------------ [...] [...] [...] [...] [...] ___________ ((*) Animals must be housed individually only in exceptional circumstances. (**) The highest point of the enclosure shall be at least 1.8 m from the floor. For marmosets and tamarines, separation from the mother should not be done before the age of 8 months. Table 6.2 Squirrel monkeys ┌ ----------------- ---------------------------------- (")" (")" | Minimum surface | Minimum volume | Minimum height | Provided date | of the floor for | for each | of the enclosure (m) | at art. 32 32 | | one (*) or two additional animal | | para. ((6) | | animals (sqm) | with age over | | from the law | | | 6 months (mc) | | | | ├ ----------------- ---------------------------------- 留言 | 加入好友 ----- | 2.0 | 0.5 | 1.8 | January 1, 2017 | └ ----------------- ---------------------------------- ️ ------------- _________ ((*) Animals must be housed individually only in exceptional circumstances. For squirrel monkeys, separation from the mother should not be done before the age of 6 months. Table 6.3 African green macaques and monkeys (*) *Font 9 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] | | Minimum surface | Minimum volume | Volume | Height | Date provided | | | of the enclosure (sqm) | of the enclosure | minimum/| minimum a | in art. 32 32 | | | | (mc) | animal | enclosure | para. ((6) | | | | | (mc) | (m) | of the law | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 | Animals aged up | | | | | | | | | | in 3 years inclusive (**) | 2.0 | 3.6 | 1.0 | 1.8 | January 1, 2017 | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | Animals with age | | | | | | | | | over 3 years (***) | 2.0 | 3.6 | 1.8 | 1.8 | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | Animals raised in | | | | | | | | | breeding purpose | | | | | | | | | | (****) | | | 3.5 | 2.0 | | | └ ---------------------- [...] [...] [...] [...] [...] [...] ____________ ((*) Animals must be housed individually only in exceptional circumstances. (**) An enclosure with minimum dimensions can be used for a maximum of 3 animals. (***) A minimum size enclosure can be used for a maximum of two animals. (****) No additional space/volume is required in breeding colonies for young animals up to 2 years of age, housed with their mother. For macaques and green monkeys, separation from the mother should not be done before the age of 8 months. Table 6.4 Babuini (*) *Font 9 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] | | Minimum surface | Minimum volume | Volume | Height | Date provided | | | of the enclosure (sqm) | of the enclosure | minimum/| minimum a | in art. 32 32 | | | | (mc) | animal | enclosure | para. ((6) | | | | | (mc) | (m) | of the law | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 | Animals aged up | | | | | | | | | | in 4 years inclusive (**) | 4,0 | 7,2 | 3,0 | 1.8 | January 1, 2017 | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | Animals with age | | | | | | | | | over 4 years (**) | 7.0 | 12.6 | 6.0 | 1.8 | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | Animals raised in | | | | | | | | | breeding purpose | | | | | | | | | | (***) | | | 12.6 | 2.8 | | | | | | | | | | | └ ---------------------- [...] [...] [...] [...] [...] [...] __________ ((*) Animals must be housed individually only in exceptional circumstances. (**) A minimum size enclosure can be used for a maximum of two animals. (***) No additional space/volume is required in breeding colonies for young animals up to 2 years of age, housed with their mother. For baboons, separation from the mother should not be done before the age of 8 months. 7. Farm animals During agricultural research, where the purpose of the research requires animals to be housed under conditions similar to housing animal housing conditions, animal housing should at least comply with the required standards. by the Veterinary Health Regulation on the protection of farmed animals, approved by Order of the President of the National Veterinary Health and Food Safety Authority no. 75/2005 , by the Veterinary Health Regulation establishing minimum standards for the protection of calves, approved by Order of the President of the National Veterinary Health and Food Safety Authority no. 72/2005 and the veterinary health rule setting minimum standards for the protection of pigs, approved by Order of the President of the National Veterinary Health and Food Safety Authority no. 202/2006 , with subsequent amendments and completions. Table 7.1 Cattle *Font 7 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] [...] | Body weight | Minimum surface | Surface | Size | Size | Date provided | | (kg) | of the enclosure | minimum of | container | container | at art. 32 32 | | | (sqm) | podelei/animal| for feeding | for ratia | para. ((6) | | | | (mp/animal) | ad libitum a | food | from the law | | | | | | | cattle without | | | | | | | | | | | | | | horns (m/ | no horns | | | | | | | animal) | (m/animal) | | | ├ ---------------------- 留言 | 加入好友 ---- -- -- -- | up to 100 inclusive | 2,50 | 2,30 | 0,10 | 0,30 | January 1, 2017 | | | | | | | | | | over 100 and | | | | | | | | | up to 200 inclusive | 4.25 | 3.40 | 0.15 | 0.50 | | | | | | | | | | | | over 200 and | | | | | | | | | up to 400 inclusive | 6,00 | 4,80 | 0,18 | 0,60 | | | | | | | | | | | | over 400 and | | | | | | | | | up to 600 inclusive | 9,00 | 7,50 | 0,21 | 0,70 | | | | | | | | | | | | over 600 and | | | | | | | | | up to 800 inclusive | 11,00 | 8,75 | 0,24 | 0,80 | | | | | | | | | | | | over 800 | 16,00 | 10,00 | 0,30 | 1,00 | | └ ---------------------- [...] [...] [...] [...] [...] [...] [...] Table 7.2 Sheep and goats *Font 7 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] [...] [...] | Body weight | Minimum surface | Minimum surface | Height | Size | Size | Date provided | | (kg) | of the enclosure | minimum animal/animal| minimum of | container | container | at art. 32 32 | | | (sqm) | (mp/animal) | boxes (m) | for feeding | for ration | para. ((6) | | | | | | | | | | | | | | | | | | | | | | | | | (m/animal) | (m/animal) | | | ├ ---------------------- [...] [...] [...] [...] [...] [...] [...] | below 20 | 1.0 | 0.7 | 1.0 | 0.10 | 0.25 | January 1, 2017 | | | | | | | | | | | over 20 and | | | | | | | | | | up to 35 inclusive | 1.5 | 1.0 | 1.2 | 0.10 | 0.30 | | | | | | | | | | | | | over 35 and | | | | | | | | | | up to 60 inclusive | 2.0 | 1.5 | 1.2 | 0.12 | 0.40 | | | | | | | | | | | | | over 60 | 3.0 | 1.8 | 1.5 | 0.12 | 0.50 | | | └ ---------------------- [...] [...] [...] [...] [...] [...] [...] [...] Table 7.3 Pigs and laboratory porcupines *Font 9 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] | Weight | Minimum surface | Minimum surface | Minimum surface | Provided date | | (kg) | of the enclosure (*) | of the floor/animal/animal/animal | at art. 32 32 | | | (sqm) | (mp/animal) | (under conditions | para. ((6) | | | | | | | neutral from point | of the law | | | | | | thermal view) | | | | | | (mp/animal) | | | ├ ---------------------- [...] [...] [...] [...] [...] [...] | up to 5 inclusive | 2.0 | 0.20 | 0.10 | | | | | | | | | | | over 5 and | | | | | | | | up to 10 inclusive | 2.0 | 0.25 | 0.11 | | | | | | | | | | | over 10 and | | | | | | | | up to 20 inclusive | 2.0 | 0.35 | 0.18 | | | | | | | | | | | over 20 and | | | | | | | | up to 30 inclusive | 2,0 | 0,50 | 0,24 | | | | | | | | | | | over 30 and | | | | | | | | up to 50 inclusive | 2.0 | 0.70 | 0.33 | | | | | | | | | | | over 50 and | | | | | | | | up to 70 inclusive | 3.0 | 0.80 | 0.41 | | | | | | | | | | | over 70 and | | | | | | | | up to 100 inclusive | 3.0 | 1.00 | 0.53 | | | | | | | | | | | over 100 and | | | | | | | | up to 150 inclusive | 4.0 | 1.35 | 0.70 | | | | | | | | | | | over 150 | 5.0 | 2.50 | 0.95 | | | | | | | | | | | Adult males | | | | | | | | | ordinary specimens) | 7.5 | | 1.30 | | | └ ---------------------- [...] [...] [...] [...] [...] [...] ___________ (*) Porcines may be isolated in smaller enclosures for short periods, for example, by compartmentalizing the main enclosure using partitions, in a situation where this measure is justified for veterinary or experimental reasons, example, if consumption of individual food is required. Table 7.4 Equine The lowest side is at least 1.5 times the height at the animal's greaban. The height of the inner sites allows the animals to stand up on their hind legs, reaching their total height. *Font 8 * ┌ ---------------------- ------------------------------------------------- | Height to greaban | Minimum floor area/animal | Height | Date provided | | (m) | (mp/animal) | minimum a | in art. 32 32 | | in -------- [...] [...] [...] [...] [...] ((6) | | | For each | For each | Fving boxes/| (m) | of the law | | | animal sheltered | animal sheltered | iapa with manz | | | | | | | | individual or | in groups of 4 | | | | | | | | in groups of | or more | | | | | | | | | up to | animals | | | | | | | | 3 animals | | | | | | | ├ ---------------------- [...] [...] [...] [...] [...] [...] | 1.00 to 1.40 | 9.0 | 6.0 | 16 | 3.00 | 1 January 2017 | | | | | | | | | | over 1.40 and | | | | | | | | | up to 1.60 inclusive | 12.0 | 9.0 | 20 | 3.00 | | | | | | | | | | | | over 1.60 | 16.0 | (2 x IG) ² (*) | 20 | 3.00 | | | | | | | | | | | └ ---------------------- [...] [...] [...] [...] [...] [...] [...] __________ (*) In order to ensure adequate space, the space standards for each individual animal must be based on the height at the greaban (IG). 8. Birds During agricultural research, where the purpose of the research requires animals to be housed under conditions similar to housing animal housing conditions, animal housing must meet at least the standards laid down. by the Veterinary Health Regulation on the protection of farmed animals, approved by Order of the President of the National Veterinary Health and Food Safety Authority no. 75/2005 , the veterinary health rule on minimum standards for the protection of laying hens, approved by Order of the President of the National Veterinary Health and Food Safety Authority no. 136/2006 , as amended, and by the Veterinary Health Rule on the establishment of minimum standards for the protection of chickens intended for meat production, approved by Order of the President of the National Veterinary Health and Food Safety Authority no. 30/2010 ,, as amended. Table 8.1 Poultry and poultry If these minimum dimensions cannot be provided for scientific reasons, the duration of the isolation must be justified by the person carrying out the procedure with the veterinary staff. In these circumstances, the birds can be kept in smaller compartments with a suitable enrichment of the living environment and with a minimum floor area of 0.75 sqm. *Font 8 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] [...] | Body weight | Minimum surface | Surface | Height | Minimum length | Provided date | | (g) | of the enclosure | minimum/bird | minimum (cm) | of the trough | to art. 32 32 | | | (sqm) | (sqm) | | food/poultry | para. ((6) | | | | | | (cm) | of the law | ├ ---------------------- [...] [...] [...] [...] [...] [...] | up to 200 inclusive | 1.00 | 0.025 | 30 | 3 | 1 January 2017 | | | | | | | | | | is 200 and | | | | | | | | | up to 300 inclusive | 1.00 | 0.03 | 30 | 3 | | | | | | | | | | | | | over 300 and | | | | | | | | | up to 600 inclusive | 1.00 | 0.05 | 40 | 7 | | | | | | | | | | | | | over 600 and | | | | | | | | | up to 1,200 including | 2.00 | 0.09 | 50 | 15 | | | | | | | | | | | | | over 1,200 and | | | | | | | | | up to 1,800 inclusive | 2.00 | 0.11 | 75 | 15 | | | | | | | | | | | | | over 1,800 and | | | | | | | | | up to 2,400 including | 2.00 | 0.13 | 75 | 15 | | | | | | | | | | | | | over 2,400 | 2.00 | 0.21 | 75 | 15 | | | | └ ---------------------- [...] [...] [...] [...] [...] [...] [...] Table 8.2 Domestic turkey The enclosure sides will have a minimum length of 1.5 m. If these minimum dimensions cannot be provided for scientific reasons, the duration of the isolation must be justified by the person carrying out the procedure with the veterinary staff. Under these conditions, the birds can be housed in smaller spaces, where there is a suitable enrichment of the living environment, and which have a minimum floor area of 0.75 sqm and a minimum height of 50 cm for the birds under 0.6 kg, 75 cm for birds under 4 kg and 100 cm respectively for birds over 4 kg. These spaces may be used for housing small groups of birds with the dimensions set out in Table 8.2. *Font 8 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] [...] | Body weight (g) | Minimum surface | Surface | Height | Minimum length of | Provided date | | | of the enclosure | minimum/bird | minimum (cm) | trough | to art. 32 32 | | | (sqm) | (sqm) | | food/poultry | para. ((6) | | | | | | (cm) | of the law | ├ ---------------------- [...] [...] [...] [...] [...] [...] | up to 0.3 inclusive | 2.00 | 0.13 | 50 | 3 | 1 January 2017 | | | | | | | | | | over 0.3 and | | | | | | | | | up to 0.6 inclusive | 2.00 | 0.17 | 50 | 7 | | | | | | | | | | | | | over 0.6 and | | | | | | | | | up to 1 inclusive | 2.00 | 0.30 | 100 | 15 | | | | | | | | | | | | over 1 and | | | | | | | | | up to 4 inclusive | 2.00 | 0.35 | 100 | 15 | | | | | | | | | | | | over 4 and | | | | | | | | | up to 8 inclusive | 2.00 | 0.40 | 100 | 15 | | | | | | | | | | | | over 8 and | | | | | | | | | up to 12 inclusive | 2.00 | 0.50 | 150 | 20 | | | | | | | | | | | | over 12 and | | | | | | | | | up to 16 inclusive | 2.00 | 0.55 | 150 | 20 | | | | | | | | | | | | over 16 and | | | | | | | | | up to 20 inclusive | 2.00 | 0.60 | 150 | 20 | | | | | | | | | | | | over 20 | 3.00 | 1.00 | 150 | 20 | | | | | | | | | | | └ ---------------------- [...] [...] [...] [...] [...] [...] [...] Table 8.3 Quails *Font 7 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] [...] [...] | Body weight | Minimum surface | Surface | Overhyped/| Minimum height | Length | Provided date | | (g) | of the enclosure | minima/bird | bird | (cm) | minimum a | in art. 32 32 | | | (sqm) | housed in additional | | | trough | para. ((6) | | | | pair (sqm) | sheltered in | | | | | | | | | | | | group (sqm) | | | | | | | | | | | | | | | | | bird (cm) | | | ├ ---------------------- [...] [...] [...] [...] [...] [...] [...] | up to 150 | 1.00 | 0.5 | 0.10 | 20 | 4 | January 1, 2017 | | including | | | | | | | | | | | | | | | | | | | over 150 | 1.00 | 0.6 | 0.15 | 30 | 4 | | | | | | | | | | | | └ ---------------------- [...] [...] [...] [...] [...] [...] [...] [...] Table 8.4 Ducks and geese If these minimum dimensions cannot be provided for scientific reasons, the duration of the isolation must be justified by the person carrying out the procedure with the veterinary staff. In these circumstances, the birds can be kept in smaller compartments with a suitable enrichment of the living environment and with a minimum floor area of 0.75 sqm. These spaces can be used to house small groups of birds, with the space standards indicated in Table 8.4. *Font 9 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] [...] | Body weight | Surface | Overhyped/| Height | Minimum length | Provided date | | (g) | minimum | bird (sqm) | minimum | of the trough | to art. 32 32 | | | of the enclosure | *) | (cm) | food/| para. ((6) | | | (sqm) | | | bird (cm) | of the law | ├ ---------------------- [...] [...] [...] [...] [...] [...] [...] | Rate | January 1, 2017 | ├ ---------------------- [...] [...] [...] [...] [...] [...] | up to 300 inclusive | 2.00 | 0.10 | 50 | 10 | | | | | over 300 and up to | | | | | | | | | 1,200 inclusive (**) | 2.00 | 0.20 | 200 | 10 | | | | over 1,200 and up to | | | | | | | | | 3,500 including | 2.00 | 0.25 | 200 | 15 | | | | over 3,500 | 2.00 | 0.50 | 200 | 15 | | | ├ ---------------------- [...] [...] [...] [...] [...] [...] | | Gaste | | ├ ---------------------- [...] [...] [...] [...] [...] [...] | up to 500 inclusive | 2.00 | 0.20 | 200 | 10 | | | | over 500 and up to | | | | | | | | | 2,000 including | 2.00 | 0.33 | 200 | 15 | | | | over 2,000 | 2.00 | 0.50 | 200 | 15 | | | └ ---------------------- [...] [...] [...] [...] [...] [...] [...] Note ... ---------- * *. This shall include a minimum pool area of 0,5 mp/2 sqm, with a minimum depth of 30 cm. The basin can occupy up to 50% of the minimum enclosure size. **) Birds that cannot yet fly can be housed in compartments with a minimum height of 75 cm. ---------- ---------- Table 8.4 of Annex 3 was amended by art. unique from LAW no. 96 96 of 7 May 2015 , published in MONITORUL OFFICIAL no. 316 316 of 8 May 2015. Table 8.5 Ducks and geese: Minimum basin dimensions (*)] ┌ ---------------------------- [...] [...] | | Surface | Depth | | | (sqm) | (cm) | ├ ---------------------------- [...] | Rate | 0.5 | 30 | ├ ---------------------------- [...] | Gaste | 0.5 | between 10 and 30 inclusive | └ ---------------------------- [...] [...] _________ (*) The dimensions of the basin are expressed/2 sqm of premises. The basin can occupy up to 50% of the minimum enclosure size. Table 8.6 Pigeons The enclosures must be long and narrow (for example, 2 m x 1 m) rather than squares so that the birds can perform short flights. *Font 8 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] [...] | Group size | Minimum surface | Height | Minimum length | Minimum length | Provided date | | | of the enclosure | minimum | of the trough | of the quench/| at art. 32 32 | | | (sqm) | (cm) | food/| bird | para. ((6) | | | | | bird (cm) | (cm) | of the law | ├ ---------------------- 留言 | 加入好友 ---- -- -- -- -- | up to 6 inclusive | 2 | 200 | 5 | 30 | January 1, 2017 | | between 7 and 12 inclusive | 3 | 200 | 5 | 30 | | | | | | | | | | | | | | | Additional | above 12 | 0.15 | | 5 | 30 | | | └ ---------------------- [...] [...] [...] [...] [...] [...] [...] Table 8.7 Cinteza zebra The enclosures must be long and narrow (for example, 2 m x 1 m) so that the birds can perform short flights. For breeding studies, couples can be housed in smaller shelters with a suitable enrichment of the living environment, with a minimum floor area of 0.5 sqm and a minimum height of 40 cm. The duration of the isolation must be justified by the person performing the procedure with the veterinary staff ┌ ----------------------------- [...] [...] [...] [...] [...] | Group size | Surface | Height | Number | Date provided | | | minimum a | minimum | minimum of | in art. 32 32 | | | enclosure | (cm) | fuel-| para. ((6) of | | | (sqm) | | | | law | ├ ----------------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | up to 6 inclusive | 1.0 | 100 | 2 | January 1, 2017 | | 7 to 12 | 1.5 | 200 | 2 | | | | 13 to 20 | 2,0 | 200 | 3 | | | | | for each bird | 0.05 | | 1/6 birds | | | Additional | over 20 | | | | | | | └ ----------------------------- [...] [...] [...] [...] [...] 9. Amphibians Table 9.1 Aquatic urodes *Font 9 * ┌ -------------------------------) ------------- [...] [...] [...] [...] | Body length (*) | Surface | Surface | Depth | Provided date | | (cm) | minimum of minimum | minimum | minimum a | at art. 32 32 para. | | | | of the area | | | (6) of the law | | | with water | water for | (cm) | | | | | (cmp) | | | | | | | | | | animal | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | (cmp) | | | | ├ ------------------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | up to 10 inclusive | 262.5 | 50 | 13 | 1 January 2017 | | over 10 and up to 15 inclusive | 525 | 110 | 13 | | | | over 15 and up to 20 inclusive | 875 | 200 | 15 | | | | over 20 and up to 30 inclusive | 1.837.5 | 440 | 15 | | | | over 30 | 3.150 | 800 | 20 | | | └ -------------------------------) ------------- [...] [...] [...] [...] _________ ((*) Measured from the head to the cloaca Table 9.2 Aquatic anure (*) *Font 9 * ┌ -------------------------------) ------------- [...] [...] [...] [...] | Body length (*) | Surface | Surface | Depth | Provided date | | (cm) | minimum of minimum | minimum | minimum a | at art. 32 32 para. | | | | of the area | | | (6) of the law | | | with water | water for | (cm) | | | | | (cmp) | | | | | | | | | | animal | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | (cmp) | | | | ├ ------------------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | under 6 | 160 | 40 | 6 | | | | | between 6 and 9 inclusive | 300 | 75 | 8 | | | | | over 9 and up to 12 inclusive | 600 | 150 | 10 | | | | | over 12 | 920 | 230 | 12,5 | | | └ -------------------------------) ------------- [...] [...] [...] [...] _____________ (*) These conditions apply to growth basins, but not to the basins used for natural mating and overovulation for reasons of efficiency, as the latter procedures require smaller individual basins. The space standards are set for adults in the indicated size categories; it is recommended either the exclusion of young animals and tadpoles or the proportional change in size. ((**) Measured from the head to the cloaca Table 9.3 Semi-aquatic anure *Font 8 * ┌ --------------------------------- (")" (")" (")" (")" (")". | Body length (*) | Surface | Surface | Height | Depth | Provided date | | (cm) | minimum of minimum | minimum | minimum of | minimum a | at art. 32 32 para. | | | | enclosure | | | (6) of the law | | | (**) | each | (***) | (cm) | | | | | (cmp) | animal | (cm) | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | (cmp) | | | | | | ├ --------------------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 | up to 5.0 inclusive | 1,500 | 200 | 20 | 10 | January 1, 2017 | | over 5.0 and up to 7.5 inclusive | 3,500 | 500 | 30 | 10 | | | | | over 7.5 | 4,000 | 700 | 30 | 15 | | | | └ --------------------------------- (")" (")" (")" (")" (")". _____________ ((*) Measured from the head to the cloaca ((**) One-third land area, two-thirds the area with sufficient water for the animals to be able to sink. ((***) Measured from the land area to the inner part of the roof of the terrarium; apart from this, the height of the enclosure must be adapted to the organization of the interior. Table 9.4 Anure Semiterestre *Font 8 * ┌ --------------------------------- (")" (")" (")" (")" (")". | Body length (*) | Surface | Surface | Height | Depth | Provided date | | (cm) | minimum of minimum | minimum | minimum of | minimum a | at art. 32 32 para. | | | | enclosure | | | (6) of the law | | | (**) | each | (***) | (cm) | | | | | (cmp) | animal | (cm) | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | (cmp) | | | | | | ├ --------------------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 | up to 5.0 inclusive | 1,500 | 200 | 20 | 10 | January 1, 2017 | | over 5.0 and up to 7.5 inclusive | 3,500 | 500 | 30 | 10 | | | | | over 7.5 | 4,000 | 700 | 30 | 15 | | | | └ --------------------------------- (")" (")" (")" (")" (")". ______________ ((*) Measured from the head to the cloaca ((**) One-third land area, two-thirds the area with sufficient water for the animals to be able to sink. ((***) Measured from the land area to the inner part of the roof of the terrarium; apart from this, the height of the enclosure must be adapted to the organization of the interior. Table 9.5 Tree anure *Font 9 * ┌ --------------------------------- [...] [...] [...] [...] [...] | Body length (*) | Surface | Surface | Height | Provided date | | (cm) | minimum of minimum | minimum | minimum a | at art. 32 32 para. | | | | | (6) of the law | | (6) of the law | | | (**) | each | (***) | | | | | (cmp) | animal | (cm) | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | (cmp) | | | | ├ --------------------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | up to 3.0 inclusive | 900 | 100 | 30 | January 1, 2017 | | over 3.0 | 1,500 | 200 | 30 | | | └ --------------------------------- [...] [...] [...] [...] [...] ___________ ((*) Measured from the head to the cloaca ((**) Two-thirds land area, one-third area of water sufficient for the animals to be able to sink. ((***) Measured from the land area to the inner part of the roof of the terrarium; apart from this, the height of the enclosure must be adapted to the organization of the interior. 10. Reptile Table 10.1 Aquatic turtles *Font 9 * ┌ -------------------------------) ------------- [...] [...] [...] [...] | Body length (*) | Surface | Surface | Depth | Provided date | | (cm) | minimum of minimum | minimum | minimum a | at art. 32 32 para. | | | | area | | area with water | (6) of the law | | | with water | water for | (cm) | | | | | (cmp) | | | | | | | | | | animal | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | (cmp) | | | | ├ ------------------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | up to 5 inclusive | 600 | 100 | 10 | January 1, 2017 | | over 5 and up to 10 inclusive | 1.600 | 300 | 15 | | | | over 10 and up to 15 inclusive | 3,500 | 600 | 20 | | | | over 15 and up to 20 inclusive | 6,000 | 1,200 | 30 | | | | over 20 and up to 30 inclusive | 10,000 | 2,000 | 35 | | | | over 30 | 20,000 | 5,000 | 40 | | | | └ -------------------------------) ------------- [...] [...] [...] [...] _____________ ((*) Measured in straight line from the front tip to the back tip of the shell Table 10.2 Terrestrial snakes *Font 9 * ┌ -------------------------------) ------------- [...] [...] [...] [...] | Body length (*) | Surface | Surface | Height | Provided date | | (cm) | minimum of minimum | minimum | minimum a | at art. 32 32 para. | | | | | (6) of the law | | (6) of the law | | | (cmp) | each | (**) | | | | | | | animal | (cm) | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | (cmp) | | | | ├ ------------------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- | up to 30 inclusive | 300 | 150 | 10 | January 1, 2017 | | over 30 and up to 40 inclusive | 400 | 200 | 12 | | | | over 40 and up to 50 inclusive | 600 | 300 | 15 | | | | over 50 and up to 75 inclusive | 1,200 | 600 | 20 | | | | over 75 | 2,500 | 1,200 | 28 | | | | └ -------------------------------) ------------- [...] [...] [...] [...] __________ ((*) Measured from head to tail ((**) Measured from the land area to the inner part of the roof of the terrarium; apart from this, the height of the enclosure must be adapted to the organization of the interior. 11. Fish 11.1. Water supply and water quality Adequate quality water flow shall be ensured at all times. Water flow from recirculation systems or filtering to basins must be sufficient and ensure that water quality parameters are maintained at acceptable levels. Water must be filtered or treated to remove harmful substances for fish if necessary. Water quality parameters shall at any time be within the acceptable range corresponding to the maintenance of normal activity and physiology for a given species and stage of development. The water flow must be adequate to allow the fish to swim correctly and maintain normal behaviour. Fish should be given an adequate period for acclimatisation and adaptation to changes in water quality conditions. 11.2. Oxygen, nitrogen compounds, pH and salinity The concentration of oxygen must be appropriate to the species and to the context in which the fish are housed. Where necessary, additional aeration of the watershed shall be ensured. The concentration of nitrogen compounds should be kept at a low level. The pH level must be adapted to the species and maintained as stable as possible. Salinity must be adapted to the requirements of fish species and fish stage of life. Changes in salinity must be done gradually. 11.3. Temperature, lighting, noise The temperature should be maintained in the optimal range for the fish species involved and as stable as possible. Changes in temperature should be done gradually. Fish shall be exposed to a period of adequate light. Noise levels shall be kept at minimum values and, where possible, equipment producing noise or vibrations, such as power generators or filtering systems, shall be separated from fish housing pools. 11.4. Stock density and environmental complexity The density of fish stock must be based on the totality of fish needs in terms of environmental conditions, health and well-being. Fish must be given a sufficient volume of water to swim normally, taking into account the size, age, health of the fish and the method of feeding them. Fish must benefit from an adequate enrichment of the living environment, such as areas of refuge or substrate, unless the behavioural characteristics suggest that none of these enrichments is necessary. 11.5. Feeding and handling Fish must benefit from an appropriate diet, feeding at an appropriate rate. Particular attention should be paid to the feeding of larval fish during any transition from natural to artificial food. Fish handling should be reduced to the bare essentials. + Annex 4 Methods of killing animals 1. The methods contained in the table below shall be used in the animal killing process. Methods other than those included in the table may be used: a) on unconscious animals, provided that those animals do not become conscious before death; ... b) on animals used in agricultural research, where the purpose of the project requires animals to be housed in conditions similar to the conditions of housing of farmed animals; those animals may be killed with the requirements of set out in Annex I to Commission Implementing Regulation (EU) No 1.099/2009 of 24 September 2009 on the protection of animals at the time of killing. ... 2. The killing of animals shall be completed using one of the following methods: a) confirmation of permanent cessation of movement; ... b) brain destruction; ... c) neck dislocation; ... d) exsanguination; or ... e) confirmation of rigor mortis installation. ... 3. Table *Font 8 * ┌ ---------------------- [...] [...] [...] [...] [...] [...] [...] [...] [...] [...] [...] | Animals-remarking/| | | | | | Dogs, | Mammal | Primate | | Methods | Pisces | Amphibians | Reptiles | Birds | Rodents | Rabbits | Cats, | great | non-human | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | Foxes | | | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 -- | Anesthetic Overdose | (1) | (1) | (1) | (1) | (1) | (1) | (1) | (1) | (1) | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 -- | Tija perforanta |-|-| (2) | | | | | | | | | | | | | | | | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 -- | Carbon dioxide | | | | | | | | | | | | | | | | | | | | | | | | | | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 -- | Cervical dislocation | | | | | | (4) | (5) | (6) | | | | | | | | | | | | | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 -- | Loving/Kick | | | | | | | | | | | | | | penetrative in the head | | | | | | (7) | (8) | (9) | (10) | | | | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 -- | Beheading | |-|-| | (11) | (12) | | | | | | | | | | | | | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 -- | Electric Asomation | (13) | (13) | | (13) | | (13) | (13) | (13) | | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 -- | Inherent gas (Ar, N 2) | | | | | | | | | | | | | | (14) | | | | ├ ---------------------- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 ----- 留言 | 加入好友 -- | Shooting with bullet | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | carabine, pistols and | | | | | | | | | | | | | | adequate ammunition | | | | | (15) | | | | | | (16) | (15) | | | | └ ---------------------- [...] [...] [...] [...] [...] [...] [...] [...] [...] [...] [...] Requirements 1. As appropriate, use after prior sedation. 2. It is used exclusively for large reptiles. 3. Use only with graduated filling. Do not use for rodents in the form of a foetus or a newborn. 4. Use only for birds of less than 1 kg. Birds over 250 g are sedated. 5. Use only for rodents of less than 1 kg. Rodents over 150 g are sedated. 6. Use only for rabbits less than 1 kg. Rabbits over 150 g are sedated. 7. Use only for birds of less than 5 kg. 8. Use only for rodents of less than 1 kg. 9. Use only for rabbits of less than 5 kg. 10. Use only for newborns. 11. Use only for birds of less than 250 g. 12. Use only if other methods are not possible. 13. A special equipment is required. 14. Use only for pigs. 15. It is used only by experienced shooters in field conditions. 16. It is used only in field conditions, by experienced shooters, in a situation where other methods are not possible. + Annex 5 LIST the elements provided in art. 22 22 para. ((6) of the Act 1. National legislation in force relevant to the acquisition, growth, care and use of animals for scientific purposes 2 2. Ethics applicable to the animal-human relationship, the intrinsic value of life and the pros and cons of the use of animals for scientific purposes 3. The fundamental and specific biology of the corresponding species correlated with anatomy, physiological characteristics, reproduction, genetics and genetic modifications 4. Animal behavior, growth techniques and improving their living environment 5. Methods of handling and species-specific procedures, as applicable 6. Animal Health Management and Hygiene 7. Recognition of manifestations of stress, pain and suffering specific to the most common laboratory species 8. Anesthesia, methods of pain mitigation and killing 9. Use of human endpoints 10 10. Requirements related to the replacement, reduction and improvement of the use of animals in procedures 11. Design of procedures and projects, as the case may be + Annex 6 LIST the elements provided in art. 36 36 para. ((2) lit. c) of law 1. Pertinence and justification of the following aspects: a) the use of animals, including the origin of the animals required, the estimated number, species ... b) procedures. ... 2. Application of replacement, reduction and improvement methods in case of animal procedures 3. Planned use of anesthesia, analgesia and other pain mitigation methods 4. Reducing, avoiding and relieving any form of animal suffering from birth to death, as appropriate 5. Use of human endpoints 6. Experimental or observational strategy and statistical design in order to minimize the number of animals, pain, suffering, stress and environmental impact, where appropriate. 7 7. Reuse of animals and the cumulative effect on animals 8. Proposed classification of the severity of procedures 9. Avoid unjustified duplication of procedures, where appropriate 10. Conditions of housing, growth and care for animals 11. Methods of killing 12. Competence of the project persons + Annex 7 Classification of severity of procedures The severity of a procedure shall be determined by the intensity of the pain, suffering, stress or lasting harm expected to be borne by an individual animal during the procedure. + Section I Categories of severity of procedures The categories of severity of the procedures are as follows: 1. No recovery: The procedures performed under general anesthesia, at the end of which the animal does not regain consciousness, are classified as without recovery. 2. Superficial: Procedures carried out on animals, which are likely to cause animals to show pain, suffering or superficial stress in the short term, as well as procedures that do not significantly affect the comfort or general condition of the animals are classified as superficial. 3. Moderate: Procedures performed on animals, which are likely to have animals present pain, suffering or moderate short-term stress or pain, suffering or superficial long-term stress, as well as procedures that may moderately affect comfort. or the general condition of the animals are classified as moderate. 4. Sever: Procedures performed on animals, which are likely to have animals present severe pain, suffering or stress or moderate long-term pain, suffering or stress, as well as procedures that can severely affect the comfort or general condition of animals are classified as severe. + Section II Award criteria The assignment of the severity category shall take into account any intervention on an animal or any manipulation thereof within the framework of a defined procedure. This is based on the most severe effects that are expected to be felt by an individual animal after applying all appropriate improvement techniques. Where a particular category is assigned to a procedure, account shall be taken of the type of procedure and a number of other factors. All these factors are analyzed on a case-by-case basis. The factors related to the procedure are mainly the following: 1. type of handling; 2. the nature of pain, suffering, stress or lasting harm caused by (all elements) the procedure (procedure), intensity, duration, frequency and multiplicity of the techniques used; 3. cumulative suffering in a procedure; 4. impossibility of expressing natural behavior, including restrictions on housing, growth and care standards. In Section III, there are examples of procedures for each category of severity based on the factors related to the procedure itself. They provide a first indication of the classification that would be most appropriate for a particular type of procedure. However, for the purposes of the final severity classification for a procedure, the following additional factors shall be taken into account, assessed on a case-by-case basis: 1. the type of species and genotype; 2. stage of development, age and sex of the animal; 3. training the animal for a certain type of procedure; 4. if the animal is to be reused, the actual degree of severity of the previous procedures; 5. the methods used to reduce or eliminate pain, suffering and stress, including improving housing conditions, growth and care; 6. Human endpoints. + Section III Examples of different types of procedures for each severity category based on factors related to the type of procedure 1. Superficial: a) the administration of anesthesia, except in situations where the sole purpose is killing; ... b) pharmacokinetic study in which a single dose is administered and a limited number of blood samples are taken (totaling < 10% of the blood volume) and where no detectable adverse effect is expected to occur; ... c) non-invasive imaging of animals (e.g. MRI) with appropriate sedation or anaesthesia; ... d) superficial procedures, for example, ear and tail biopsies, non-surgical subcutaneous implantation of minipompals and microchips; ... e) the use of external telemetry equipment which only causes minor discomfort to animals or interferes with their activity and their normal behaviour; ... f) administration of substances by subcutaneous, intramuscular, intraperitoneal pathways, by gavage and intravenously through superficial blood vessels, if the substance has no more than a superficial impact on the animal, and the volumes are within the limits appropriate to the size and species of the animal; ... g) induction of tumors or spontaneous tumors, which do not cause detectable adverse clinical effects (e.g., small, subcutaneous, non-invasive nodules); ... h) breeding of genetically modified animals, in order to obtain a superficial affected phenotype; ... i) feeding on modified diets, which do not meet all the nutritional requirements of the animal and which are expected to cause a superficial clinical abnormality during the study; ... j) short-term immobilization (< 24 h) in metabolic cages; ... k) behavioral studies involving short-term deprivation of social partners and isolation of adult individuals from sociable species (mice and rats); ... l) models that expose animals to harmful stimuli that are immediately associated with a superficial level of pain, suffering or stress and that animals can successfully avoid; ... m) a combination or a combination of the following examples may result in the classification as "superficial": ... ((i) assessment of the body's composition by non-invasive measurements and minimal immobilization; (ii) the monitoring of the ECG by non-invasive techniques with minimal immobilisation or without the immobilization of the animals used (iii) the use of external telemetry equipment which is expected not to cause any discomfort to socially adapted animals and which does not interfere with normal activity and normal behaviour; ((iv) the breeding of genetically modified animals not expected to have any detectable negative clinical phenotype; (v) the addition of markers inherent in food to trace the route of food during digestion; ((vi) deprivation of forced food for < 24 h in adult rats; (vii) open field testing. 2. Moderate: a) the frequent application of test substances that produce moderate clinical effects and the sampling of blood samples (> 10% of the blood volume) from a conscious animal during a few days without the replacement of the blood volume; ... b) studies to establish concentrations that produce acute toxicity, chronic toxicity/carcinogenicity tests, leading to the conclusion of the procedure without the death of the animal; ... c) surgical interventions performed under general anesthesia and with appropriate analgesics, associated with pain, suffering or with impairment of postoperative general condition. Examples include: thoracotomy, craniotomy, laparotomy, orchidectomy, lymphadenectomy, thyroidectomy, orthopedic surgery with effective stabilization and wound management, organ transplantation with effective rejection management, surgical implant of catheters or biomedical equipment (e.g. remote transmission equipment, minipomps, etc.); ... d) models for the induction of tumors or spontaneous tumors, likely to cause pain or moderate stress or to moderate the normal behavior; ... e) irradiation or chemotherapy with a subletal dose or a normally fatal dose, but with reconstitution of the immune system. The expected adverse effects are superficial or moderate and limited over time (< 5 days); ... f) breeding of genetically modified animals in order to obtain a moderately affected phenotype; ... g) creation of genetically modified animals through surgical procedures; ... h) use of metabolic cages involving moderate restriction of movements over prolonged duration (up to 5 days); ... i) studies performed under modified diets, which do not meet all the nutritional requirements of the animal and are expected to cause a moderate clinical abnormality during the study; ... j) deprivation of forced food for 48 h in the case of adult rats; ... k) triggering escape reactions and avoidance reactions in which the animal is unable to escape or avoid the stimulus and which is expected to produce moderate stress. ... 3. Sever: a) toxicity tests in which the endpoint is death or likely to cause death from accidents and in which severe pathophysiological conditions are induced. For example, single-dose acute toxicity testing (see Organisation for Economic Co-operation and Development guidelines on experiments); ... b) testing a device if its failure can cause severe pain or stress or the death of the animal (e.g. cardiac assistance devices); ... c) to test the effectiveness of a vaccine characterised by the persistent manner in the general condition of the animal, the progressive disease leading to death, associated with long-lasting moderate pain, stress or suffering; ... d) irradiation or chemotherapy with a lethal dose without reconstitution of the immune system or reconstitution with the occurrence of graft reaction disease; ... e) models with induced tumors or with spontaneous tumors, which are expected to cause a progressively fatal illness associated with pain, stress or long-lasting suffering. For example, tumors that cause casexy, invasive bone tumors, tumors with metastatic spread and tumors with ulceration; ... f) surgical and other interventions performed on animals under general anesthesia that are expected to cause pain, suffering or moderately persistent postoperative stress or to affect the severe and persistent manner of general condition of animals. The production of unstable fractures, thoracotomy without the use of appropriate analgesics or trauma that induce the insufficiency of several organs; ... g) organ transplantation where rejection of the organ can lead to severe stress or severe impairment of the general condition of the animal (e.g., xenotransplantation); ... h) breeding animals with genetic malformations likely to cause severe and persistent impairment of the general condition, e.g. Huntington's disease, muscular dystrophy, recurrent chronic neuritis patterns; ... i) the use of metabolic cages which involves the severe limitation of freedom of movement for prolonged duration; ... j) electric shock that cannot be avoided by the animal (for example, to produce a newfound helplessness); ... k) complete isolation over prolonged periods of social species, for example, non-human dogs or primates; ... l) stress caused by immobilization to induce gastric ulcer or cardiac arrest in rats; ... m) the tests of swimming or forced exercise which have as the end point the exhaustion. ... ------
