Cabinet of Ministers Regulations No. 204, Riga, 30 March 2004 (pr. Nr. 17, § 14) veterinary requirements of semen, ova and embryos of trade Issued in accordance with article 25 of the law of veterinary medicine of i. General questions 1. establishes the health requirements of semen, ova and embryos of trade with Member States of the European Union (hereinafter referred to as Member States), and with third countries.
2. the terms used in the rules: 2.1. sperm — prepared or diluted ejaculate of an animal;
2.2. collection Center-food and veterinary service recognised and supervised business, in which semen is produced for use in artificial insemination;
2.3. semen storage centre, the food and veterinary service recognised and supervised establishment which stores the sperm for artificial insemination;
2.4. the Centre's veterinarian, veterinarian, who is responsible for the collection of the Centre is complying with the requirements of these regulations;
2.5. sperm loads a single veterinary certificate for semen quantity specified;
2.6. the national collection of the semen, the Member State or third country in which the finished, collected, processed and, if necessary, store the semen, ova and embryos, to send to another Member State;
2.7. ievākum — semen quantity at one time is obtained from the donor;
2.8. veterinary control: any physical or administrative control formalities applicable animal semen, ova and embryos to directly or indirectly protect human or animal health;
2.9. official veterinarian: a food and veterinary service of the veterinary inspector or a food and veterinary service official;
2.10. a third country — a country which is not a Member State;
2.11. the embryo: the initial stage of development of the animal while it is capable to recipient;
2.12. embryo collection team, food and veterinary service officially approved group of technicians or supervised by an official veterinarian of the Group entity that is competent to obtain, process and store embryos;
2.13. the consignment of embryos — a quantity of embryos removed in one operation from a single donor and covered one of the veterinary health certificate.
3. the rules do not apply to: 3.1 the zootechnical requirements artificial insemination and semen distribution organisation;
3.2. the embryos obtained, by transferring the nuclei.
4. Artificial insemination and trade with Member States only allowed to use the cow semen and ova where: 4.1 obtained and processed in the center of the collection of the semen and semen stored in a storage centre;
4.2. obtained from animals whose health status complies with the requirements of chapter V of the rules;
4.3. meet the requirements of this regulation.
5. Artificial insemination and trade with Member States only allowed to use the porcine semen and ova where: 5.1 obtain and handle in the center of the collection of the semen;
5.2. derived from pigs whose health status complies with this provision in Chapter VII;
5.3. meeting the requirements of this regulation.
6. Artificial insemination and trade with Member States permitted to use only one of the ovine, caprine and Equine semen that: 6.1. acquired and processed in the center of the collection of the semen or from ovine and caprine animals-farm;
6.2. conformity with the requirements of these provisions.
7. food and veterinary department recognize semen collection or storage centre if it complies with the requirements of this regulation.
8. Embryo Transplantation and to trade with the Member States may use only such species of cow embryos: 8.1 or artificial insemination get fertilized in vitro with sperm from the donor of the semen Center, or by semen imported in the territory of the European Union in accordance with the requirements of this regulation;
8.2. derived from cow animal species whose health status complies with the provisions of chapter XII;
8.3. for, processed and stored by embryo collection team in accordance with the rules laid down in chapter XII.
9. Ovine, caprine, porcine and equine species, ova and embryos: 9.1 retrieves the embryo collection team;
9.2. remove from a female donor animals which comply with the provisions of Chapter XVI;
9.3. processing laboratory.
10. the semen used ovine, caprine and equine species female donor insemination, complies with this provision in point 6.
11. food and veterinary department recognize the embryo collection team, if it complies with this provision the requirements laid down in chapter XI.
12. If a change of the embryos of the Group shall notify the food and veterinary service. The food and veterinary service shall be amended by the embryo collection team recognition documentation if: 12.1. the Group has changed the veterinarian;
12.2. the organisation of the group or the laboratory equipment or significant changes are made.
13. the semen, ova or embryos of destination shall notify by means of the Animo system (computerised system linking veterinary authorities in the countries to facilitate the exchange of information between the country of dispatch and of destination of the accompanying document to the competent authorities of the country).
14. The food and veterinary service veterinarian supervises the implementation of these provisions and, if the requirements of this regulation are not fulfilled, reverses the decision of the center of the collection of the semen, semen storage centres or collection groups.
15. food and veterinary service shall record all of the semen or semen storage centres and embryo collection teams, and grants them a registration number.
16. These provisions in certain studies carried out national veterinary diagnostic Center. If the national veterinary diagnostic center of one of the studies not conducted, it shall send the relevant sample to a recognised laboratory in another Member State.
17. in the collection of the semen and semen storage centres, embryo collection team as well as a list of the food and veterinary service, send the other Member States and the European Commission. The said institutions shall also be notified of any withdrawal of opinion.

18. The food and veterinary service to authorize the importation of semen and embryos in Latvia, the presentation of an animal health certificate in accordance with the provisions of 1, 2, or 3. the annex of another Member State has drawn up by the official veterinarian. Semen and embryos may leave Latvia to another Member State, if the official veterinarian of the country has produced these rules 1, 2, or 3. referred to in the annex to the veterinary health certificate. The veterinary health certificate (original) is sent along with the cargo to the destination of the semen and meet the following requirements: 18.1. it shall be drawn up in at least one of the collection's official languages and in one of the official languages of the country of destination;
18.2. it takes one page or one single document.
19. food and veterinary service does not let the cargo in Latvia, if the document is found during the inspection it does not comply with the requirements of this regulation.
20. The food and veterinary service shall take the necessary measures (including storage in quarantine), if there is a suspicion of contamination or pollution of the semen with pathogenic organisms.
21. where these rules 19 and 20 in the cases provided for in the semen importation prohibited cargo in Latvia, after the sperm loads at the request of the consignor or his representative, authorise the return of the semen, the consignor, if it is not contrary to the laws and regulations laying down the animal health requirements.
22. If the country of collection of the semen within 30 days do not give permission to send a shipment of semen back, the food and veterinary service issued an order for the destruction of this semen. The food and veterinary service of the decision taken (justified) shall inform the consignor of the semen or his representative.
II. Imports from third countries of semen and embryos 23 may be imported only from those third countries and those of the semen or storage centres and embryo collection teams, of which the European Commission has published in the official journal of the European Union on the list.
24. the embryos may be imported from third countries where the third-country requirements for tuberculosis and enzootic bovine leucosis are the same or stricter than that laid down in laws and regulations concerning veterinary requirements for bovine and porcine movement (which introduces the Council 26 June 1964 directives 64/432/EEC on animal health problems affecting intra-Community trade in bovine animals and swine (hereinafter Directive 64/432), (A) and (G) the requirements laid down in the annexes).
25. the embryos may be imported from third countries, if the donor is from a holding in which no animal has been vaccinated against foot-and-mouth disease for at least 30 days prior to embryo collection and not subject to any prohibition or quarantine measures. From third countries where vaccination against foot-and-mouth disease, may only be imported into the frozen embryos. In the case of frozen embryos stored for at least 30 days prior to shipment.
26. The food and veterinary service to authorize the importation of semen and embryos only on presentation of an animal health certificate drawn up and signed by the official veterinarian of the third country. The veterinary health certificate (original) is sent along with the cargo to the destination and meet the following requirements: 26.1. it shall be drawn up at least in one of the official languages of the country of destination and in one of the official languages of the Member State where the semen and embryos of control;
26.2. it only takes one page or one single document;
26.3. This is just one consignee.
27. food and veterinary service checks each boar semen imported into Latvia's cargo before it is put into circulation, or undergo a semen shipment customs procedure and does not allow it to be imported when the audit reveals that: 27.1. shipment of semen from a third country or collection centre, which does not include this provision in paragraph 23 on the list;
27.2. sperm loads attached to the veterinary health certificate does not conform to the rules in paragraph 26.
28. food and veterinary service may take the necessary measures (including storage in quarantine), to determine whether semen is suspected of being infected or contaminated by pathogenic organisms, provided that this does not affect the viability of the semen.
29. If the admission of semen load in the country is prohibited under any of the provisions referred to in paragraph 27 of the conditions of the semen and 30 days in the country do not give permission to send deep freeze semen back, the food and veterinary service issued an order for the destruction of this semen. If sent fresh semen loads, such order shall be issued immediately.
30. If a decision on destruction of semen, all relevant costs shall be borne by the consignor, the consignee or his representative.
31. The food and veterinary service may require a veterinary health certificate attesting compliance with these provisions, if the semen, ova and embryos in Latvia introduced through the territory of a third country.
32. The boar semen in trade with Member States apply safeguard measures in accordance with the legislation on veterinary and zootechnical checks trade with Member States of the European Union (which introduces the Council on 26 June 1990 directive 90/425/EEC concerning veterinary and zootechnical checks applicable in intra-Community trade in certain live animals and products with a view to the completion of the internal market, article 10 required).
33. If the third country is causing and spreading of infectious animal disease which can be transmitted by semen and which may threaten Latvia's herds, food and veterinary service shall prohibit the introduction of semen from the whole of the third country or a part of its territory, whether or not semen is imported directly or indirectly through another Member State. The food and veterinary service shall inform the other Member States and the European Commission.
III. Collection Center 34. conditions for recognition of the semen Center constantly working in food and veterinary service authorised Centre veterinarian.
35. Of the semen is: 21.8. animal housing including isolation facilities;
35.2. collection of space, including a separate room for the cleaning and disinfecting or sterilization;
35.3. the semen processing room (may be located elsewhere);
35.4. a semen storage room (may be located elsewhere);
22.1. the isolation of separate storage, which has no direct contact with any other animal buildings.
36. the collection Center premises comply with the following requirements:

36.1. the contact with animals outside;
36.2. animal housing and semen collection, processing and storage facilities are easy to clean and disinfect;
36.3. animal housing is separated from the semen processing room and both in turn separated from the semen storage room.
37. Semen storage centre: 37.1. permanent work in the food and veterinary service official;
37.2. the contact with animals outside;
37.3. storage rooms are easy to clean and disinfect.
IV. Collection and storage centre supervision conditions 38. Collection Center: 38.1. may stay only for the collection of the semen for animals. Other animals can be found in the Centre only if they do not pose a risk of infection to those species whose semen is to be collected, and they meet all of the conditions laid down by the Centre veterinarian;
38.2. the unauthorised entry and residence. Visitors who stay in the Centre is authorized to execute the conditions of the Centre veterinarian;
23.8. working staff that are adequately trained in disinfection procedures and hygiene measures;
23.9. is available on the documentation containing the following information: in the center of each 38.4.1. existing animal species, race, date of birth and identification;
38.4.2. all movement of animals imported into or out from the Center;
38.4.3. all of the animals present in the Centre of the analysis and the results of diagnostic, treatment and vaccination;
38.4.4. collection and processing date;
38.4.5. the destination of the semen;
38.4.6. information about the storage of semen;
38.5. the official veterinarian verifies the collection centres animal breeding period and assessed the compliance of the Centre the approval and supervision conditions: 38.5.1. at least once a year, if the Center hosts seasonal animal reproductive system;
38.5.2. at least twice a year, if the Center hosts seasonal animal with the reproductive system;
24.0. the accommodation for the horse which has a common area with artificial insemination or breeding centers: 38.6.1. may import mares and stallions for natural breeding, if they comply with these rules, 134.1, 134.2.134.3. and the requirements of subparagraph 134.4.;
38.6.2. are individual instruments and equipment for artificial insemination or natural breeding;
36.6. each individual dose of semen is identified in an indelible manner so as to make it possible to establish its Member State of origin, the date of acquisition, the species, the identity of the donor animal and the name of the approved centres, and the number in which the semen is collected.
39. Collection and storage centre shall ensure that the monitoring of the processing and storage of semen, it does not come in contact with the other consignment of semen.
40. the collection, processing and storage takes place in rooms that are intended for this purpose and which comply with the hygiene conditions.
41. All equipment, collection and processing time come into contact with the semen or the donor animal, before they are disinfected or sterilized, or use single-use instruments.
42. the Semen used in the processing of animal products, including additives or a diluent-are obtained from sources which do not pose a risk for animal health, or is it processed that this risk is completely eliminated.
43. Storage containers and transport containers before each refill is disinfected or sterilized or disposable containers are used.
44. Permitted Use cryogenic who have not previously used for other products of animal origin.
45. Each individual dose of semen is clearly marked in such a way as to be easily detectable, the date of collection of the semen, the breed and identification of the donor animal, as well as the approval number of the Centre. The food and veterinary service shall notify the European Commission and the other Member States of its territory in the form of the marking and introduced form.
46. If the semen collection Center also stores, the Center must comply with the provisions referred to in paragraph 38.
47. Sperm that is found in the Center, can be processed in approved centres if the acquisition: 29.3. This semen is obtained from cow species that meet the requirements of chapter V of the rules;
47.2. a semen processing is carried out with separate equipment or at other times than semen intended for trade with Member States, and the equipment is cleaned after use and sterilized;
47.3. the semen is not marketed in the Member States, it does not come into contact with the Member States and not the semen is stored along with it;
47.4. such semen is identified with a label that is different from the market in the Member States of the marking of the semen.
48. Semen storage centre: 48.1. the donor Bull is controlled health and listed in the Centre and from imported semen exported in accordance with the laws and regulations on health rules applicable to frozen semen of domestic animals of the bovine trade (which introduces the Council on 14 June 1988 Directive 88/407/EEC concerning the animal health conditions applicable to frozen semen of domestic animals of the bovine domestic trade in and imports into the community);
48.2. the official veterinarian at least twice a year, check the center of the conditions of approval and supervision;
48.3. the unauthorised entry and residence. Visitors who stay in the Centre is authorized to execute the conditions of the Centre veterinarian;
30.1. the working staff that are adequately trained in disinfection procedures and hygiene measures;
30.1. stored only semen collected semen collection centres approved in accordance with these terms, and it does not come into contact with any other semen.
49. In approved semen storage centres may be accepted only from approved semen collection or storage centres, if during transport it provide health guarantees and it has not been in contact with any other semen.
50. Deep-frozen embryos may be stored in approved semen storage centres where: 50.1. storage has allowed food and veterinary service;
50.2. embryos comply with the legislation on animal health requirements for intra-EU trade in embryos of domestic animals of the bovine species (which introduces the Council on 25 September 1989 directive 89/556/EEC on animal health conditions governing intra-Community trade in embryos of domestic animals of the bovine species, and imports from third countries);

50.3. embryos stored in separate containers in rooms that have been identified for the storage of semen.
V. provisions applicable to bovine species accepted for insertion in collection centres 51. animals at least 28 days in quarantine shed where there has declared a food and veterinary service, with the exception of the quarantine may be other cloven-hoofed animals having at least the same health status.
52. Before entering the quarantine accommodation of the animals in the herd, which is staying in the tuberkulozesbrīv and brucelozesbrīv according to the laws and regulations concerning veterinary requirements for bovine and porcine movement (which implements the requirements of Directive 64/432). Animals not previously kept in herds where the animal health situation is worse than the above.
53. The animals come from herds that are officially enzootic-bovine-leikozesbrīv according to the laws and regulations concerning veterinary requirements for bovine and porcine movement (which implements the requirements of Directive 64/432), or were born to mothers of bulls, which are selected according to the legislation on animal health problems affecting the trade in bovine animals and swine the European Union (which implements the requirements of Directive 64/432).
54. In the case of animals derived embryo transplantation and embryo recipient's mother, semen of bulls not placed on the market until the donor has reached the age of two years and a negative result obtained.
55.28 days before the period of quarantine for animals are subjected to the following tests (except bovine viral diarrhoea in antibody analysis), and the results are negative: 55.1. bovine tuberculosis: tuberculin subcutaneous analysis is carried out in accordance with the laws and regulations on animal health problems affecting the trade in bovine animals and swine the European Union (which implements Directive 64/432, annex B);
55.2. bovine brucellosis, a serological analysis is carried out in accordance with the laws and regulations on animal health problems affecting the trade in bovine animals and swine the European Union (which introduces the annex of Directive 64/432 C requirements);
55.3. enzootic bovine leukosis, a serological analysis is carried out in accordance with the laws and regulations concerning veterinary requirements for bovine and porcine movement (which implements Directive 64/432, annex D, chapter II);
55.4. enzootic bovine rhinotracheitis/Pustular vulvovaginitis: serological blood sample analysis has been performed, if the animals have been brought in from a herd which is enzootic in Pustular infectious bovine rhinotracheitis/vulvovaginītabrīv;
55.5. bovine viral diarrhoea in: 55.5.1. made a virus isolation or virus Antigen analysis analysis;
55.5.2. performed serological analysis to determine the presence of antibodies or the lack of it.
56. food and veterinary service takes this rule 55, paragraph analysis samples from animals in quarantine headquarters, where the results will become known before the start of the quarantine period.
57. If any of the tests found a positive result, the animal concerned shall be removed from the quarantine partition. If quarantine contains a group of animals, quarantine time for the remaining animals can not begin until the separation of seropositive animals.
58. The quarantine period the animals after at least seven days in quarantine investigates to determine the Campylobacter are venereal and m. feta Trichomonas foetus.
59. in order to determine the Campylobacter feta to venereal, m. performs one of the following examinations: 59.1. in the case of animals less than six months or they since six months old before the quarantine is single sex group investigates one of the vagina nomazgājum sample or one prepuciāl sample;
59.2. If the animals are six months old or older, and before the quarantine is contacts with female animals, three times with an interval of one week investigating the vagina prepuciālo nomazgājum samples or samples.
60. in order to determine the Trichomonas foetus, take one of the following examinations: 60.1. If the animals are younger than six months, or six months of age it since before the quarantine is single sex group investigates one of the model prepuciāl;
60.2. If the animals are six months old or older, and before the quarantine is contacts with female animals, three times with an interval of one week investigating the prepuciālo samples.
61. If any of these 59 or 60 of the rules referred to in paragraph results is positive, the animal shall be immediately removed from the quarantine shed. If the insulation is the Group of animals, food and veterinary service shall take the necessary measures to restore the fitness of animals to insert in the collection.
62. The quarantine period the animals in quarantine after at least 21 days spent investigating to determine the bovine brucellosis, bovine rhinotracheitis and bovine viral diarrhoea.
63. in order to determine the bovine brucellosis, a serological analysis conducted according to the laws and regulations concerning veterinary requirements for bovine and porcine movement (which implements Directive 64/432, annex C).
64. in order to determine the bovine rhinotracheitis, take blood samples for serological analysis.
65. If any of these rules and paragraph 64 63. the results of the analyses referred to in is positive, the animal shall be immediately removed from the quarantine and other animals in the group not later than 21 days after removal of the animals out of the analysis.
66. in order to determine the bovine viral diarrhoea, performs the following analysis: 66.1. virus isolation or virus Antigen analysis analysis;
66.2. the serological analysis to determine the presence of antibodies or the lack of it.
67. Only if it occurs in animals that seroconversion serological analysis before entering the quarantine station was negative, collection of the semen in the room are authorized to import any animal (seronegative or seropositive). If all the animals of the seroconversion occurs, who remained in quarantine in seronegative, are kept longer, up to three weeks, the group no longer happens seroconversion.
68. before sending the semen obtained from bovine viral diarrhoea in serologically positive bulls, from samples taken each animal checked by virus isolation or virus antigen ELISA analysis to determine the bovine viral diarrhoea. If positive results are obtained, the Bulls were removed from the center of the collection of the semen and all this bull semen destroyed.
69. animals may be accepted only in the collection centre with the express permission of the Centre veterinarian. The importation of animals of the semen in the Center and out of it.

70. the collection of the semen in the center of the animals on the day of adoption must not include any clinical signs of disease. All the animals are shipped from this provision in paragraph 52 of the quarantine accommodation, which on the day of dispatch shall satisfy the following conditions: 70.1. it occupies an area of 10 kilometres radius in which for at least 30 days have not found any cases of foot-and-mouth disease;
70.2. shed at least three months, have not found any cases of foot-and-mouth disease or brucellosis;
70.3. shed at least 30 days have not found any disease to bovine diseases for which adequate legislation on veterinary requirements of the bovine and porcine movement (which introduce Annex E to Directive 64/432) is compulsory.
71. If you meet the animal health conditions and the provisions referred to in paragraph 55 of the tests performed during the previous 12 months, animals may be directly moved (without isolation or testing) from one collection centre to another of the semen Center, in which the animals have the same health status. These animals must not come into direct or indirect contact with other cloven-hoofed animals of a lower health status. Vehicles sanitized before use. Animals from the collection Center in Latvia moved to the collection centre in another Member State (or vice versa) according to the legislation on veterinary requirements of the bovine and porcine movement (which implements the requirements of Directive 64/432).
Vi. Bovine animals to tests of the semen in the recognised centres 72. All bovine animals that are kept in semen collection centres recognised, at least once a year the following analysis is carried out and the results obtained are negative: 72.1. bovine tuberculosis: tuberculin subcutaneous analysis is carried out in accordance with the laws and regulations concerning veterinary requirements for bovine and porcine movement (which implements Directive 64/432, annex B);
72.2. bovine brucellosis, a serological analysis is carried out in accordance with the laws and regulations concerning veterinary requirements for bovine and porcine movement (which implements Directive 64/432, annex C requirements);
72.3. enzootic bovine leukosis, a serological analysis is performed according to the laws and regulations concerning veterinary requirements for bovine and porcine movement (which implements Directive 64/432, annex D, chapter II);
72.4. enzootic bovine rhinotracheitis, is performed in the blood sample analysis;
72.5. bovine viral diarrhoea — in serological analysis is applied only to seronegative animals. If an animal become serologically positive, then all this animal's ejaculate, obtained since the last negative test shall be disposed or examined. Virus test result must be negative;
m. venereal to 72.6 at.-Campylobacter feta is made in prepuciāl sample analysis. Check only the bulls, from which the semen is collected or which deal with the bulls, from which the semen is collected. Bulls returning to collection of the semen, after a break of six months are checked not less than 30 days prior to resuming production;
Trichomonas foetus 72.7. — prepuciāl is carried out the analysis of the sample. Check only the bulls, from which the semen is collected or which deal with the bulls, from which the semen is collected. Bulls returning to collection of the semen, after a break of six months are checked not less than 30 days prior to resuming production.
73. If any of the provisions referred to in paragraph 72 results is positive, the animal shall be isolated and the semen that has been obtained since the last negative test may not be used in trade with Member States, with the exception of each ejaculate of semen is checked to detect the virus, NGQ and the result is negative. Another center of semen collected after the date the analysis was carried out with positive results, should be kept separate, and must not be used for trade with Member States, until the Center is restored to health status.
VII. conditions of the porcine species in the collection to insert the recognized centres 74. animals of the porcine species for at least 30 days in quarantine shed where there has declared a food and veterinary service in which the other animals with at least the same health status.
75. before the arrival of the animals that rule 74 within the quarantined herds or their designates accommodation: 75.1. which has been free of brucellosis;
75.2. the past 12 months which have not found against foot-and-mouth disease in the vaccinated animals;
75.3. that during the previous 12 months established for Aujeszky's disease serological or virological presence;
75.4. which is not in the restriction zone.
76. The animals must not be above herds where the animal health situation is worse.
77. before the provisions referred to in paragraph 74 of the period and within the previous 30 days, the animals are subjected to the following tests, and the results are negative: 77.1. Brucella Antigen analysis of brucellosis;
77.2. analysis of serum neutralization or imunofermentatīv analysis (IFA or ELISA), using all the Aujeszky's disease viral antigens not vaccinated pigs;
77.3. Elisa analysis to identify for Aujeszky's disease G1 antigens in the case of pigs vaccinated with a G1 deleted vaccine; highlighted
77.4. The Elisa or serum neutralization analysis analysis to detect the presence of classical swine fever.
78. If the animal brucellosis test results are positive, then the animals, with negative results, this shed adopt quarantine after confirming the absence of brucellosis their animal herds or holdings, of which the results are positive.
79. The food and veterinary service permits the analysis referred to in this chapter to perform the quarantine accommodation, if the results are known before this provision set out in paragraph 74 of the 30-day quarantine period.
80. The quarantine for the first 15 days of such analysis is carried out and the results obtained are negative: 80.1. Brucella Antigen analysis of brucellosis;
80.2. a serum neutralisation or an Elisa analysis analysis using all the Aujeszky's disease viral antigens not vaccinated pigs, or an Elisa analysis to identify for Aujeszky's disease G1 antigens in the case of pigs vaccinated with a G1 highlighted the vaccine.

81. If any of the provisions referred to in paragraph 80 of the results is positive, the animal shall be removed immediately from the quarantine accommodation. The food and veterinary service shall take all measures necessary to ensure the remainder of satisfactory health status before they are placed in the collection centre in accordance with the requirements of this regulation.
82. If the animal brucellosis test results are positive, the following procedures shall apply: 82.1. positive sera are performed on the sero-agglutination analysis, as well as Brucella Antigen analysis, if it is not carried out;
82.2. the epidemiological tested their animal holding that the analysis results are positive;
82.3. animals of the test results are positive, is carried out by recycling a brucella Antigen analysis as well as eroaglutinācij and complement fixation test analysis. Samples are taken for more than seven days after the first sampling;
82.4. suspicion of brucellosis are approved or rejected, having regard to the holding of origin inspection results and their comparison of the results of the test series;
82.5. If the suspicion of brucellosis is not confirmed, that the test results were negative brucellosis test series first, you can insert a semen collection centre. Animals that were positive in only one of the results of the analysis, you can insert a Center, if the results were negative in two series of analyses carried out at least seven day interval, agglutination with Rose-bengal, and sero-agglutination of antigens and complement fixation test analysis.
83. animals may be accepted only in the collection centre with the express permission of the Centre veterinarian. The importation of animals of the semen in the Center and out of it.
84. Collection centres within the adopted animals must not show any clinical signs of the disease. All the animals are shipped from this provision referred to in paragraph 74 of the quarantine accommodation, which on the day of dispatch shall satisfy the following conditions: 84.1. not in the restriction zone;
52.3. shed in the last 30 days is not registered, a pathological clinical or serological presence of Aujeszky's disease.
85. If these provisions are complied with the conditions referred to in paragraph 84, and those tests have been carried out during the previous 12 months, animals may be directly moved (without isolation or testing) from one approved semen collection centre to another of the semen Center, in which the animals have the same health status. These animals must not come into direct or indirect contact with other cloven-hoofed animals of a lower health status. Vehicles sanitized before use. Trade with the Member States, the animals shall not be required by a veterinary health certificate that conforms to the laws and regulations concerning veterinary requirements for bovine and porcine movement (which implements Directive 64/432 2. Annex F model).
VIII. Statutory standard analyses that are carried out in an approved semen collection centre contains animals of porcine species 86. All animals of the porcine species kept in semen collection centres recognised, such analysis is carried out and the results obtained are negative: 86.1. a serum neutralisation or an Elisa analysis analysis using all the Aujeszky's disease viral antigens not vaccinated pigs, or an Elisa analysis to identify for Aujeszky's disease G1 antigens in pigs which have been vaccinated with a G1 deleted vaccine; highlighted
86.2. Brucella Antigen analysis of brucellosis;
86.3. imunofermentatīv analysis (IFA or ELISA) or serum neutralization analysis to detect the presence of classical swine fever.
87. These tests shall be carried out either on all animals when they leave the collection centre (but not later than 12 months after taking it, if they do not leave until the Center), and samples may be taken in a slaughterhouse, or 25% of the semen of the animals present in the Centre investigates each month. In this case, the Centre veterinarian shall ensure that samples are taken from all boars accommodation and all age groups. The Centre veterinarian shall ensure that each animal to investigate at least once while staying in the center of the collection of the semen, and at least once every 12 months, if the Center staying longer than a year.
88. If any of the provisions referred to in paragraph 86 results is positive, the animal quarantines and semen is obtained since the last negative test may not be used in trade with Member States. Other collection centres animal semen collected after the date the analysis was carried out with positive results, may not be used in trade with Member States.
IX. Conditions recognised collection of boar semen obtained from the Centre for trade with Member States 89. Semen is from boars: 89.1. that on the day of collection of the semen have no clinical signs of disease;
89.2. you have not been vaccinated against foot-and-mouth disease;
89.3. conforming to this chapter VII;
89.4. which are not used for natural mating during natural;
89.5. which are kept in the collection of the semen in the Center: 89.5.1. which is not located in an area subject to restrictions pursuant to laws and regulations on pig infectious diseases;
89.5.2. in the 30 days prior to collection of the semen of boars have been free of slimībasbrīv.
90. the semen after final dilution or diluent is added in an effective combination of antibiotics, in particular against leptospirosis and mikoplazmoz. If the semen is frozen, antibiotics, added before the semen freezing. A combination of antibiotics to be with like effect as the solution for which is not less than 500 ml streptomycin, 500 IU penicillin g, 150 g, lincomycin, 300 g spectinomicīn. Immediately after the addition of the antibiotics the diluted semen stored in at least 15 ° C for not less than 45 minutes.
91. Semen intended for trade with Member States: 91.1. before sending stored according to this provision the requirements of chapters III and IV;
91.2. transported to the Member State of destination in sealed containers before use has been cleaned and disinfected or sterilized.
92. If Latvia is free of slimībasbrīv, a food and veterinary service will not accept the introduction of semen from collection centres which take vaccinated against Aujeszky's disease boars.
X bulls semen. conditions for trade with Member States 93. Semen intended for trade with Member States, has been obtained from animals: 93.1. that on the day of collection of the semen have no clinical signs of disease;

93.2. which have not been vaccinated against foot-and-mouth disease during the 12 months prior to collection of the semen. If the animals have been vaccinated against foot-and-mouth disease during the 12 months prior to collection, in which case 5% (with a minimum 5 there) from each of the resultant dose investigates with virus isolation in the analysis to detect foot-and-mouth disease. The results obtained must be negative;
93.3. prior to collection of the semen have been kept on approved collection centre for at least 30 days, if you get the fresh semen;
93.4. recognised since the admission of the semen is used for natural mating during the natural;
93.5. which are kept in the collection of the semen in the Center: located at 93.5.1. an area of 10 kilometres radius in which for at least 30 days have not found any cases of foot-and-mouth disease;
93.5.2. in which has not been free of foot-and-mouth disease for at least three months prior to collection of the semen and 30 days after collection or, if sent to the fresh semen, until the date of dispatch;
93.5.3. in which 30 days prior to collection of the semen and 30 days after collection of the semen or, if sent to the fresh semen, until the date of dispatch, no species of cow disease compulsorily notifiable under the laws and regulations concerning veterinary requirements for bovine and porcine movement (which implements Directive 64/432 E requirements of chapter I of the annex).
94. the semen added antibiotics to their concentration in the diluted semen would be the following: 94.1 Ķg streptomycin per ml 500. final dilution;
94.2.500 IU (international units) of penicillin per ml final dilution;
150 ml of lincomycin Ķg 94.3. final dilution;
Ķg 300 ml spectinomycin 94.4. final dilution.
95. the semen may add another combination of antibiotics with similar effect against kampilobaktērij, leptospirosis and mikoplazmoz.
96. Immediately after the addition of the antibiotics the diluted semen stored in at least 5 ° C for not less than 45 minutes.
97. Semen intended for trade with Member States: 60.3. stored frozen for at least 30 days prior to shipment. This requirement does not apply to fresh semen;
97.2. transported to the Member State of destination and numbered in the sealed containers before use is cleaned and disinfected or sterilized.
XI. Embryo collection and preparation group 98. conditions for recognition are embryos collected, processed and stored by embryo collection team veterinarian or under its responsibility, by one or more technicians who are trained by the veterinarian.
99. the embryo collection team is under the supervision of the official veterinarian, and exposure.
100. the embryo collection team is permanent or mobile laboratory facilities where embryos are examined, processed and packed and comprising at least a work surface, microscope and cryogenic equipment.
101. The permanent laboratory are: the space in which the 101.1. work is carried out with the embryos and next to the room in which the embryos are obtained from the donor animals;
101.2. room with equipment for collection and processing of instruments and devices for cleaning and sterilisation;
101.3. laminar flow equipment, if you perform the embryo micromanipulation which involves penetration of the zona pellucida. Equipment cleaned and disinfected batch processing in between.
102. mobile laboratory consists of two separate parts: the 102.1. one section is intended for testing and embryo handling (clean);
102.2. the second part in contact with the donor animals used equipment and materials.
103. to embryo production team should prepare and process in vitro fertilization embryos obtained, the following additional requirements: 103.1. staff is trained in disease control and laboratory analyses, in particular for sterile conditions;
103.2. Group has a permanent laboratory where the processing is: 103.2.1. the equipment and facilities, including a separate room for the ova from the ovaries, a separate room for the processing of ova and embryos and embryo storage space;
103.2.2. laminar flow equipment, handling eggs, sperm and embryos. Centrifugation of semen may be carried out outside the laminar flow equipment, observing hygiene preventive measures.
104. where ova and other tissues are obtained in a slaughterhouse, the groups have the appropriate equipment for ovarian and other tissue retrieval and delivery to the processing laboratory in a hygienic and safe manner.
105. mobile laboratory shall liaise with the permanent laboratory to ensure the sterilization of equipment and security with liquids and other products necessary for the collection and activities with them.
XII. Collection, preparation, handling, storage and shipping conditions for embryos obtained 106. and recognized the embryo collection team that does not come into contact with consignments of embryos not meeting the requirements of this regulation.
107. the embryos obtained from other rooms or parts of the isolated farm in easy to clean and disinfect the area.
108. The embryos shall be processed (examined, washed, placed in labelled and sterile containers) or a mobile lab, which is not located in a zone subject to prohibition or quarantine measures.
109. all accessories that come into contact with or donor embryos before being disinfected or sterilized, or use single-use instruments.
110. the products of animal origin used in the embryo collection and transport, are derived from sources which do not pose a threat to health, or that the processing before use, to prevent such threats.
111. All features and solutions used for the collection of ova and embryos, processing (inspection, cleaning and enrichment), canning or freezing, sterilized. The funds allowed to add antibiotics.
112. Storage flasks and transport flasks disinfected or sterilized before the commencement of each filling.
113. Use cryogenic feature not previously used for other products of animal origin.
114. each embryo container or containers in which they are stored and transported, are marked with the code to be able to determine the date of collection, the species, and the registration number of the group, as well as the identification of the donor animal parents.
115. wash each embryo according to internationally recognised procedures at least 10 times in a special fluid for embryos, which changes each time and containing trypsin. Each wash solution is the solution to one hundred times the previous solution, and fetal movement every time I use a sterile micropipette. At the same time may be washed only ova and embryos from the same donor.

116. After the last wash each embryo shall be subject to microscopic examination with at least 50 times magnification, to determine whether the zona pellucida is not damaged and is exempt from any material.
117. Any micromanipulation after the last wash and examination, which involves penetration of the zona pellucida, carried out for this purpose. Such micromanipulation may only embryos with intact zona pellucida is.
118. Each consignment of embryos that have undergone this provision provided for in paragraph 116 test, placed in a sterile container that is labelled in accordance with the provisions of paragraph 114, and immediately sealed.
119. Each embryo shall, if necessary, be frozen as soon as possible and stored in a place which is under the supervision of an official veterinarian and which is subject to regular official veterinary checks.
120. Allow the rinse and detergent solution may contain trypsin. You can also use the enzyme trypsin treatment.
121. the embryo collection team regularly submits the rinsing solution, washing solution, split the fertilized ova and embryo samples for bacterial and viral official inspection.
122. Embryo collection embryo collection team registered in the 12 months before and 12 months after storage, including: 122.1. the donor animal species, age and identification;
122.2. collection, processing and storage space;
122.3. identification of embryos and details of their destination if known;
122.4. details of micromanipulation techniques that involve penetration of the zona pellucida or other techniques (such as in vitro fertilization or in vitro cultivation). In vitro fertilization embryos obtained in May to highlight the entire consignment of embryos, including news on the ovaries and/or egg collection date and location. Also provides the ability to identify the herd of origin of the donor animal.
123. This provision 106, 107, 108, 109, 110, 111.112.113, 115, 114, 116, 117, 118, 119, 120.., 121 and 122. requirements applicable to ovarian, eggs and other in vitro fertilization or in vitro cultivation of tissue to be used in production, processing and transportation. In addition, the following additional conditions: 123.1. where the ovaries and other tissues are obtained at the slaughterhouse, slaughterhouse must be a food and veterinary service officially approved and under official veterinary supervision, which is responsible for the death of the donor animal and the post-mortem inspection;
123.2. materials and equipment that come in direct contact with the ovaries and other tissues, sterilize before use and after sterilization is used only for these purposes. Different donor of ova and embryos of a batch processing uses a separate facilities;
123.3. ovaries and other tissues shall not be taken into the processing lab, pending all this day post-mortem inspection of animals slaughtered. If the donor or any party on the same day, the slaughtered animal diseases are diagnosed, all the tissues in the lot destroyed;
123.4. washing and examination procedure provided for in this provision in paragraph 116 and 117, carried out after completion of production procedure;
123.5. any micromanipulation which involves penetration of the zona pellucida shall be carried out in accordance with the provisions of paragraph 116 and 117 conditions at 123.4. the procedure provided for in subparagraph;
in one ampoule or 123.6 tube may be stored embryos from the same donor party.
124. each embryo collection team shall ensure the storage of the embryos at an appropriate temperature in premises approved for this purpose by the competent authority of the Member State concerned.
125. Recognized the embryo storage facilities shall meet the following requirements: 125.1. have at least one lockable room intended only for storage of embryos;
125.2. the rooms are easy to clean and disinfect;
125.3. spaces are continuously received and sent to the registry of the embryo that contains the destination of the embryo;
125.4. premises are subject to veterinary checks the official.
126. The food and veterinary service permission to store semen, the premises which comply with the legislation on animal health conditions applicable to frozen semen of domestic animals of the bovine species for trade with Member States of the European Union (which introduces the Council on 14 June 1988 Directive 88/407/EEC concerning the animal health conditions applicable to frozen semen of domestic animals of the bovine domestic trade in and imports into the community).
127. for trade in embryos from the approved storage premises until their arrival at the destination for transported in sealed containers under hygienic conditions.
128. the number which is marked on the tank, is equal to the number on the veterinary health certificate.
XIII. the conditions for the species embryo donor cow in 129. Donor, from which embryos meet the following requirements: 129.1. at least the previous six months in the territory of the European Union or a third country;
129.2. they are in the herd of origin for at least 30 days prior to collection of the embryos;
the herd of origin of 129.3. is: 129.3.1. officially tuberkulozesbrīv;
129.3.2. official or brucelozesbrīv brucelozesbrīv;
129.3.3. enzootic bovine leikozesbrīv. The donor may have come from a herd which is enzootic bovine leikozesbrīv, but which have been received over the last three years there has not observed any clinical enzootic bovine leucosis;
129.4. during the previous year they have remained in the herd, which is not observed in clinical infectious bovine rhinotracheitis.
130. on the day of embryo collection the donor cow: 130.1. is kept in a holding which is not subject to veterinary prohibition or quarantine measures;
130.2. the donor cow no clinical signs of disease.
131.129 and 130 these provisions referred to in paragraph conditions apply to live animals intended as donors of eggs and remove them eggs or ovarian.
132. At the slaughterhouse after slaughter the resulting eggs and other tissues of the donor animal must not be intended for slaughter under a national programme for the eradication of the disease, and they must not come from a holding subject to restrictions for animal diseases.
133. Slaughterhouse, in which the ovaries and other tissues shall not be located in areas subject to prohibition or quarantine measures.
XIV. the conditions for the Stallion semen collection centres recognised for 134. Sperm retrieval may be used only stallions who meet the following requirements: 134.1. putting it at the Centre, as well as those on the day of collection of the semen must not be any signs of infectious disease;

134.2. they come from a Member State or third country, or part of the territory of the country and of the nursery, which is located under the supervision of an official veterinarian and comply with the laws and regulations on health conditions of the equine species, and for the transport of animals imported from third countries (which introduces the Council on 26 June 1990 directive 90/426/EEC on animal health conditions governing the movement of animals of the equine species, and imports from third countries);
134.3.30 days prior to collection of the semen have been kept on the farm, they where not one of the equine species animals during that period is not observed in equine viral arthritis clinical signs;
134.4.60 days prior to collection of the semen have been kept on the farm, they where not one of the equine species animals during that period have been contagious equine metritis of clinical signs;
134.5.30 days prior to collection of the semen and they are not used during copulation;
they made the following 134.6. study, and the results are negative: 134.6.1. agar-gel immuno-diffusion method (Coggins test) to detect equine infectious anaemia;
134.6.2. a serum neutralisation test for equine viral arthritis. If the serum dilution of 1 in 4 is positive, then the entire quantity of semen taken from a donor, do a virus isolation test for equine viral arthritis specific;
134.6.3. contagious equine metritis carried out the test on two occasions with an interval of seven days by isolating the taylorella equigenitalis from pre-ejaculatory fluid or a semen sample and from genital swabs taken from the penile sheath, urethra and urethral;
134.7. they are subject to one of the following testing programmes: 134.7.1. If trade in fresh or chilled semen and 134.7.1.1. If the donor Stallion: at least 30 days prior to the first semen collection and during the collection has consistently stayed in the center of the collection of the semen, and no animal of a species of horse semen collection centre does not come in direct contact with the horse, animals of a lower health status than the donor Stallion the test laid down in these rules, 134.6 take at least 14 days after the start of permanent residence and at least once a year at the beginning of the breeding season;
134.7.1.2. If the donor Stallion reside in the center of the collection of the semen, and/or other animals of the equine species in the center of the collection of the semen have come in direct contact with the horse, animals of a lower health status, the tests laid down in this provision in paragraph 134.6 14 days prior to the first semen collection and at least once a year at the beginning of the breeding season. As laid down in this rule 134.6.1., at least once every 120 days during the period of collection of the semen. The test set out in these terms, taken in 134.6.2, not later than 30 days before each of the semen, unless a virus isolation test which must be carried out each year, does not endorse that seropositive Stallion for equine viral arthritis is not a Distributor;
134.7.2. If the trade with frozen semen, the test programme as specified in these rules or in 134.7.1 from the mandatory 30 days storage of semen and not less than 14 days after collection of the semen from carrying out the tests laid down in this provision, 134.6. irrespective of his residence status.
XV. the conditions for the teķ and goat semen getting recognised centres 135. Of the semen may only be used for the teķ and ibex from centres or holdings which, in the opinion of the official veterinarian on the day of collection of the semen is clinically healthy and meet the requirements of the legislation on animal health requirements for trade in ovine and caprine animals (which introduces the Council on 28 January 1991 Council Directive 91/68/EEC on animal health conditions governing intra-Community trade in ovine and caprine animals (hereinafter referred to as Directive 91/68), 4, 5 and 6 of the article).
136. The animals in the 30 days prior to collection of the semen, are carried out such tests, and the results are negative: 136.1. the test of brucellosis (b. melitensis) in accordance with the legislation on animal health requirements for trade in ovine and caprine animals (91/68 directive introduces Annex C);
136.2. test to detect contagious epididymitis (b. Ovis) in accordance with the legislation on animal health requirements for trade in ovine and caprine animals (91/68 directive introduces Annex D);
136.3. test to determine enzootic ovine abortion virus.
137. Teķ and buck are performed during the tests or checks designed to guarantee compliance with the requirements of these regulations and section 136.2.136.1..
138. If any of the provisions referred to in paragraph 136 tests is positive, the animal quarantines and semen is obtained since the last negative test may not be used for marketing. Trade should not be used for other animal semen, obtained after the date on which the test was carried out with positive results, if animals are susceptible to the disease.
XVI. the conditions of females on a recognized donor semen collection centres 139. Female animals may be used for the taking of ova or embryos only if they come from flocks which, after the opinion of the official veterinarian, meet the requirements of the legislation on trade in live animals between Member States of the European Union.
140. the female pig in the collection to insert the recognized centres apply the legislation on veterinary requirements for bovine and porcine movement (which implements Directive 64/432).
141. Female ovine and caprine animals into semen collection centres recognised example of the legislation on animal health requirements for trade in ovine and caprine animals (which implement Directive 91/68).
142. the animals of the equine species in addition to the requirements laid down in the laws on health conditions for the carriage of the equine species, and imports from third countries (which introduces the Council on 26 June 1990 directive 90/426/EEC on animal health conditions governing the movement of animals of the equine species, and imports from third countries) before the taking of ova or embryos in the 60 day nurseries in which there have been contagious equine metritis of clinical signs and those 30 days prior to the acquisition of ova or embryos not used in copulation.
XVII. final question

143. the rules shall enter into force on 1 May 2004.
Informative reference to European Union directives, the regulations include provisions resulting from: 1) Council of 14 June 1988 Directive 88/407/EEC laying down the animal health conditions applicable to frozen semen of domestic animals of the bovine domestic trade in and imports into the community;
2) Council 25 September 1989 directive 89/556/EEC on animal health conditions governing intra-Community trade in embryos of domestic animals of the bovine species, and imports from third countries;
3) Council 26 June 1990 directive 90/429/EEC laying down the animal health requirements applicable to intra-Community trade in and imports of semen of domestic animals of the porcine species;
4) Council on 13 July 1992 Directive 92/65/EEC laying down the health requirements for the animals, semen, ova and embryos of the trade in and imports into the community, not subject to animal health requirements laid down in specific Community rules referred to in Directive 90/425/EEC in Annex A (I).
Prime Minister i. Emsis Minister of Agriculture m. Roze Editorial Note: rules shall enter into force on 1 May 2004.
 


 
 


 
 
 


 
Minister of agriculture m. rose