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Cabinet of Ministers Regulations No. 234 in Riga in 2005 (April 5. No 17 21) amendments to the Cabinet of Ministers of 20 April 2004 the Regulation No 334 "rules on food contamination and corrosive chemical substances in the food packaging and marking" Issued under the food surveillance law article 4, second subparagraph, and article 13 of the third to make the Cabinet of 20 April 2004 the Regulation No 334 "rules on food contamination and corrosive chemical substances in the food packaging and labelling" (Latvian journal 66., 2004, nr.) the following amendments: 1. provisions be supplemented by 7.1 points as follows: "7.1 determination of the erucic acid content in oils and fats intended usage of the nai consumption and in foodstuffs containing added oils or fats, use that rule 6. test method referred to in the annex." 2. Delete paragraph 8, the words and figures "laid down in these rules 1, 2, 3, 4 and 5 in the annex". 3. Deleting the introductory part of paragraph 16, the words "national". 4. Delete paragraph 17, the number and the word "13 and". 5. Make the informative reference to European Union directives as follows: "Informative reference to European Union directives, the regulations include provisions resulting from: 1) of the Council of 20 July 1976 Directive 76/621/EEC relating to the fixing of the maximum level of erucic acid in oils and fats intended as such for human consumption and in foodstuffs containing added oils or fats; 2) Commission 25 July 1980 of Directive 80/891/EEC relating to the Community method of analysis for determining the erucic acid content in oils and fats intended as such for human consumption and in foodstuffs containing added oils or fats; 3) Council of 20 December 1985 Directive 85/591/EEC concerning the introduction of Community methods of sampling and analysis for the monitoring of foodstuffs intended for human consumption. " 6. To supplement the provisions of annex 6 by the following: "6. the annex to Cabinet of Ministers of 20 April 2004, Regulation No 334 of the test method for the determination of the erucic acid content in oils and fats intended as such for human consumption and in foodstuffs containing added oils or fats i. General questions 1. when preparing the sample, the following requirements shall be met: 1. take a sample in the laboratory whose mass is not less than 50 g; 1.2. the sample is homogenized before analysis; 1.3. to prevent contamination of the sample, prepared sample is stored automatically in the closed air herm and moisture-tight container. 2. the reagents Used shall meet the following requirements: 2.1 for solution, dilution or washing purposes use distilled water or demineralized water with the same purity as distilled water. If it is mentioned in the "solution" or "dilution" without specifying the specific reagents, it must understand the water solution or dilution with water; 2.2. all chemical reagents are analytical reagent quality unless legislation provides otherwise. 3. Equipment list includes only the items that have the specified usage and specification. The analytical sensitivity of the weight is at least 0.1 mg. 4. The result is expressed as the total percentage of the mass of fatty acids in the sample, which is received in the laboratory, unless otherwise specified. The test report contains the result is the average value obtained at least two determinations with satisfactory repeatability. II. the erucic acid content of the detection method and principle 5. determines the erucic acid content in oils and fats that contain cetoleīnskāb, as well as hide the rogenēt oils and fats containing trans and cis isomers of dokozēnskāb. Oil and fat components: methyl esters of fatty acids, separated using thin layer chromatography method. For media use with silver nitrate (AgNO3) treated silica gel. The process is happening at low temperature, and kvantit methyl esters of active content is determined using gas-liquid chromatography method. 6. If the oil and fats are fatty acids that make up analysed using gas chromatography method, it is not possible to separate them from the rest of erucic acid dokozēn acid ISO measures, therefore, first determine the total contents of total dokozēnskāb or cis-dokozēnskāb content in accordance with the requirements set out in the Commission's 11 July 1991 of Regulation (EEC) no 2568/91 on the olive oil and olive-residue oil and on the relevant methods of analysis (hereinafter referred to as Commission Regulation No 2568/91/EEC) in annex 10, under the analysis of the fatty acid methyl esters by gas chromatography. 7. methyl esters of fatty acids are obtained in accordance with the requirements set out in the Commission's May 6 2002, Regulation (EC) No 796/2002 amending Regulation (EEC) no 2568/91 on the olive oil and olive-residue oil and on the relevant methods of analysis and Council Regulation (EEC) No 2658/87 on the tariff and statistical nomenclature and on the common customs tariff of the additional notes of the annex (hereinafter referred to as Commission Regulation No 796/2002/EC) Annex 10 B section for the acquisition of the methyl esters of fatty acids from olive oil and olive-pomace oils. 8. the samples shall be taken in accordance with the requirements laid down by the Commission on 6 November 2003, the Regulation No 1989/2003 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis (hereinafter: Commission Regulation (EC) No 1989/2003/EC) (A) of annex 1 section. III. Reagents and equipment for the determination of the erucic acid content of 9. determination of the erucic acid content, the following reagents: 9.1. fresh, clean distilled ethyl ether (no peroxide impurities); 9.2. n-hexane; 9.3. silica gel G thin layer chromatography; 9.4. silica gel column chromatography; 9.5. silver nitrate solution 200 mg/l (dissolve 24 g of silver nitrate (AgNO3) and make up with water to 120 ml); 9.6. methyl erucate solution 5 mg/ml (in a few ml of n-hexane to dissolve 50 mg methyl erucate, and make up to volume with n-hexane to 10 ml); 9.7. metiltetrakozanāt 0.25 mg/ml of the standard solution (in a few ml of n-hexane to dissolve 25 mg metiltetrakozanāt and make up with n-hexane to 100 ml); 9.8. development solvent toluene and n-hexane 90:10 respectively: (v/v); 22.214.171.124-dichlorofluorescein solution 0.5 g/l (heating and stirring dissolve 50 mg of 2.7-dichlorofluorescein 100 ml of 50% methanol aqueous solution). 10. the determination of the erucic acid content uses the following equipment: 10.1 facilities 10.1.1. thin-layer chromatography: cold-developing tank and all content maintained temperature from-20 ° C to-25 ° C; 10.1.2. glass plates 200 x 200 mm, 10.1.3. UV lamp; 10.1.4. approximately 200 mm long glass column internal diameter — about 10 mm with glass wool or sintered glass filter funnels or with sintered glass filters; 10.1.5. applicator spotting on TLC plates narrow band or strip; 10.2 gas chromatograph in accordance with the requirements set out in Commission Regulation No 2568/91/EEC, in section A of annex 10. IV. Progress 11. For the preparation of methyl esters of fatty acids analysis in ga look for having about 400 mg of oil or fat ingredients and prepare a solution containing about 20-50 mg/ml of the fatty acid methyl esters of n-hexane pursuant to the methods set out in the waters of Commission Regulation No 796/2002/EC under heading B of the annex 10 of the methyl esters of fatty acids from olive oil and olive-pomace oils. 12. Tlc plates begin with preparation. 500 ml round flask having 60 g silica gel (5.8), add 120 ml of silver nitrate (AgNO3) solution (9.5) and shake for one minute until a natural mass. The resulting mass is applied to the glass plates so that the layer thickness is approximately 0.5 mm. Such a mass quantity is sufficient to prepare a 200 x 200 mm of five plates. Plates let air-dry (preferably leave them in the dark for about 30 minutes). Then the plate fully dry and activate, insert oven 100 ° C for two hours and 30 minutes. Use the plate immediately after activation or carefully store in a dark place and activate again before use. One hour activation at 110 ° C can be considered satisfactory provided the plates are not darkened activation. Before the use of plates through the entire thickness of 10 mm from the edges of each plate and the top of the intake line to reduce the development time of edge effects. 13. use of methyl esters added applicator (10.1.5). The narrow, about 50 mm long, at least 40 mm in from the edge of the plate and 10 mm from the bottom plate spotted on 50 µ l methyl ester (11) made from look ga. Similarly, deposit 100 µ l of a solution containing equal the prepared solution of methyl esters (11) and methyl erukāt (9.6). With the solution do the spotting during special care due to the brittleness of the coating. If you want, you can put on the plate to methyl erukāt 50 µ l solution (9.6), after developing the plate to help you identify the methyl erucate band. The bottom edge of the plate after spotting the methyl esters diethyl ether until the leaves ether ascends approximately 5 mm above the loading area of the sample, thus the methyl esters concentrate at a narrow band. 14. to develop the plate, developing container about 5 mm deep valley the developing solvent (9.8) and placed in the Chamber. Place the camera in cold storage-25 ° C (10.1.1. bottom) or the possible closer temperature. Tank from the inside can spread out through paper. After two hours the plate carefully insert the tank and allow the solvent to ascend to the development of two-thirds of the height of the plate. Remove the plate and gently evaporate in a nitrogen stream from the solvent. Place the plate in the tank and allow the solvent to ascend to the top plate. Remove the plate, nitrogen stream and is evaporated carefully sprayed with the solution of 2.7-di hlorfluoresceīn (9.9). Then into the plate under UV light and determines the location of the bar containing methyl erucate in the sample. The report's paragraph having intensified in the zone of the sample, which is attached to the methyl erucate band. 15. To separate the methyl ester fractions, 50 ml beaker quantitatively (trying to avoid losses) scrape and carry the methyl erucate band derived from the sample. Similarly, in another 50 ml beaker, transfer the silica gel located above and below the methyl erukāt bar. This bar contains all other fractions of methyl esters of fatty acids. In each beaker add 1.0 ml of standard solution of metiltetrakozanāt (9.7) and 10 ml of diethyl ether (9.1). Mix well and transfer the contents of the beakers in separate columns or funnels (10.1.4), each of which contains one gram silica gel (9.4), and into the methyl esters using three or four 10 ml portions of diethyl ether. Collect the filtrates in small flasks. Evaporate the filtrate of each small volume using a mild nitrogen jets. Methyl esters are moved to the small glass tubes with a sharp tip. The solvent is separated, the evaporating nitrogen stream, thus the methyl esters are concentrated in the bottom of the tubes. Dissolve the methyl esters in the 25-50 µ l of n-hexane (9.2). 16. gas-liquid chromatography separates 16.1: methyl esters in accordance with the method laid down in Commission Regulation No 2568/91/EEC, in section A of annex 10 on the analysis of the fatty acid methyl esters by gas chromatography. Then analyzes the 1-2 µ l of the methyl ester solutions obtained from the fraction containing methyl erucate, and from fractions of methylated fatty acids balance; 16.2. the fraction containing methyl erucate, from the electronic integrator the chromatogram of the sample the following peak areas: 16.2.1. methyl erucate (E); 16.2.2. internal standard (L1) (internal standard used in accordance with the Commission Regulation 2568/91/EEC in annex X A bottom point down 126.96.36.199. in question required); 16.2.3. methyl esters total bar area without internal standard (EF); from the chromatogram of the fraction of 16.3. containing the remaining methyl esters of fatty acids reads the following: 16.3.1. square bar of the methyl esters of the total bar area without internal standard (RF); 16.3.2. internal standard (L2) (internal standard used in accordance with the Commission Regulation 2568/91/EEC in annex X A bottom point down 188.8.131.52 requires good at). V. test results the test results 17 is replaced by the following: 17.1. the erucic acid content of the sample, expressed as a percentage of the methyl esters of the total fatty acid methyl esters (prepared from the sample) is calculated using the following formula: E/L1 [(EF/L1) + (RF/L2)] x 100 where E, EF, RF, L1 and L2: a bar area (16.2 and 16.3), which adjusts as necessary, using the calibration factors. For practical purposes the methyl erucate band value derived using the formula referred to in this subparagraph shall be equal to the erucic acid content, expressed as a percentage of the total quantity of fatty acids in the sample (assume that the contents of the sample tetrakozānskāb is trivial); 17.2. If the bar area are expressed in percentage, size EF and RF is calculated as follows: EF = RF = 100-100-L1 L2 17.3. If the contents of the tetrakozānskāb look work is considerable, tetrakozānskāb (L2) the value obtained from the lowest fraction of hromat which content of methyl esters of fatty acids balance, reduced to: L2-T2 where T2 = T0 x P2/P0, where T2 — metiltetrakozanāt bar area obtained from the sample and forms part of the bar area, which is attributed to the hromat gramm factions remaining methyl esters of fatty acids of the internal standard; P2-metilpalmitāt bar area obtained from the chromatogram of the remaining fraction; T0-metiltetrakozanāt bar area, which is derived from the methyl esters of the total fatty acids of the chromatograms. Analysis of methyl esters are laid down in Commission Regulation No 2568/91/EEC, in section A of annex 10 of the fatty acid methyl ester analysis with gas chromatography; P0-metilpalmitāt bar area obtained from the chromatogram of the methyl esters of fatty acids of common, established through analysis that laid down in Commission Regulation No 2568/91/EEC, in section A of annex 10 on the analysis of the fatty acid methyl esters by gas chromatography; 17.4. the proportion of fatty acids in the fraction containing the methyl erucate, expressed as a percentage of the total fatty acids in the fraction, calculated as follows: 17.4.1. [(EF/L1)/(EF/RF/L2 L1 +)] x 100 or [EF/L1 (L1 + L2 EF/RF/)] x 100 17.4.2. the proportion of erucic acid fraction containing methyl erucate, calculated as follows: E/EF 17.4.3. the erucic acid content of the sample, expressed as a percentage of total fatty acids, is calculated as follows : [EF/L1 (L1 + L2 EF/RF/)] x E/EF x 100 or [E/L1 (L1 + L2 EF/RF/)] x 100 10.9. determining the repeatability, the difference between the results of two determinations on the same sample, simultaneously or one immediately after the other under the same conditions stated by the same analyst shall not exceed 10% of the result or 0.5 g per 100 g of the sample (the highest value). 18. If the fraction is called methyl erucate, it can contain other monoēnskābj of methyl esters, but no metilcetoleāt. It is not necessary to determine the level of erucic acid in oils and fats, as well as in foodstuffs containing added oils and fats, if, using the Select method of analysis (Commission Regulation (EC) No 796/2002 annex X), found that they contain no more than 5% of total total dokozēnskābj or cis-dokozēnskābj. "
Prime Minister a. Halloween Minister of Agriculture m. rose
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