Cabinet of Ministers Regulations No. 296 in 2005 Riga April 26 (Mon. No 23 33) control of potato wart disease and combat order Issued in accordance with the Cabinet of Ministers of 14 law equipment of the first paragraph of article 3 i. General questions 1. determines the order in which prejudice and combating plant quarantine organisms — potato — the spread of cancer. 2. the national plant protection service (hereinafter service): 2.1 monitor and control the spread of potato wart disease and determine the distribution of potato wart disease containment and control measures (hereinafter referred to as phytosanitary measures);
2.2. laboratory diagnosed cancer of the potato. 3. a Person must not store or replicate the agent of potato wart (Synchytrium endobioticum) pure cultures, except for the legislation concerning the trial or scientific purposes and for work on varietal selections of harmful organisms, plants, plant products and contact with them came the arrival of the items and the order in certain cases. II. determination of phytosanitary measures 4. potato potato field or the lot is infected with potato wart, if symptoms are detected at least one plant or tuber. 5. If the agent of potato wart (Synchytrium endobioticum), service shall decide on the following: phytosanitary measures 5.1. determine infected: 5.1.1 field where the potatoes were grown and the infected field boundaries highlights the plan;
5.1.2. the potato lot, which found the infected tuber;
5.2. the buffer zone established around the infected area and highlights its boundary plan. The size of the buffer zone shall be determined taking into account the biological organisms of plant quarantine conditions to protect the surrounding area from contamination;
5.3. instructs the infected field owner or holder to: 5.3.1. processing Potato tubers and Greens to destroy agents of potato wart;
5.3.2. disinfect machinery and equipment each time the infected field after use;
5.4. prohibited in infected fields owner or holder to the infected in the field for 20 years to grow or to store: 5.4.1. potatoes and other plants of the family Solonacea;
5.4.2. plants intended for replanting. 6. the buffer zone is the area in which the plant quarantine organism is not present or is being eradicated and that includes infected area or clings to it or plant quarantine organism from free sites, or production, or production unit, which is not present in the plant quarantine organism and which measures are taken to prevent a particular plant quarantine organism. 7. Buffer zone may be grown potato varieties that are in accordance with the catalogue of plant varieties considered to be resistant to the potato wart race found infected in the field. 8. Service: 8.1. the decision shall specify the deadline of phytosanitary measures;
8.2. the phytosanitary measures taken to monitor and control. III. Phytosanitary measures 9. Service cancellation shall take a decision on the abolition of certain phytosanitary measures if Infected field agent of potato wart (Synchytrium endobioticum) is not found. 10. the service shall decide on the specific phytosanitary measures permitting the partial removal of the infected field owner or holder against the infected field grow potato wart resistant potato varieties for food, if: 10.1. at least 10 years after the phytosanitary measures;
10.2. the infected field 10 years has cultivated and it is not grown perennials lawn;
10.3. biological soil sample test result is negative and direct in mikroskopēšan per gram soil found in the less than five viable sporangij. 11. to check compliance with this provision of the soil 10.3. the requirements referred to in point, service take soil samples (per ha in the field 0.33 takes one sample, which consists of 60 cores) and it laboratory analysis carried out in accordance with the provisions of the methods referred to in the annex. 12. If the direct mikroskopēšan of the soil sample in one gram of soil in more than five found viable or sporangij using the organic method of a positive result, the service shall decide on the continuation of certain phytosanitary measures and carried out a field test after two or more years, taking into account the degree of infection or the found plate number of sporangij. 13. the service shall take a decision on the abolition of certain phytosanitary measures if: 13.1. at least 20 years after the phytosanitary measures;
13.2. the field 20 years has cultivated and it is not grown perennials lawn;
13.3. soil samples in the immediate mikroskopēšan soil samples found no viable sporangij and biological examinations found no disease of potatoes. If the mikroskopēšan is established to direct viable sporangij, biological testing may be omitted. 14. to verify compliance with this provision of the soil 13.3. the requirements referred to in point, service take soil samples (out of every single model of 0.33 hectares consisting of 60 cores) and it laboratory analysis carried out in accordance with the provisions of the methods referred to in the annex. 15. If any of the laboratory analyses revealed the agent of potato wart (Synchytrium endobioticum), service shall decide on the continuation of certain phytosanitary measures and the rules referred to in paragraph 4, the phytosanitary measures for another three years. 16. following the abolition of the phytosanitary measures the service harvest potatoes varieties tested in the first harvest, which is susceptible to the potato wart. A person who cultivates against cancer-susceptible potato potato varieties, 10 working days before harvest, inform the service. Informative reference to European Union directives, the regulations include provisions deriving from Council of 8 December 1969 Directive 69/464/EEC on cancer control. Prime Minister a. Minister of Agriculture Halloween — Minister of finance Spurdziņš o. Annex Cabinet of 26 April 2005, regulations no 296 soil sample testing methods of agent of potato wart Synchytrium endobioticum detection 1. Direct mikroskopēšan: 1.1 dried (air-dry) 100 g soil samples 24 hours suspended in 900 ml of running water. All parts fully mixed soil; 1.2. the suspension is passed through a sieve with an electromagnetic Shaker 500, 250, 125, 71, 40 and 25 µm sieve; 1.3. from 40 to 25 µm sieve rinse, dried on filter paper and move the 50 ml centrifuge tubes; 1.4. each tube Valley chloroform (15 ml) of concentrated solution of CaCl2, or mix and centrifuge the tubes at speeds of 800 g for 15 minutes; 1.5. the solution above the sediment filter through filter paper. Rinsing with chloroform or CaCl2 is repeated two more times, or until no longer float the particles; 1.6. the amount collected on filter paper with 1 ml of diluted laktoglicerol (or more, if necessary) and s. endobioticum snaudsporangij inspection microscope; 1.7. calculation of sporangij counts and their number per gram soil; 1.8. sporangij, filled with a granular gray mass or easily round the other plasma (if seen sprouting), regarded as viable, but plazmolizēt or unclear content should be considered dead. Some of the sporangij in viable fluoresces blue light. 2. biological testing: 2.1. organic inspection use against s. endobioticum races held at gas potato varieties. It is therefore important to know the natural infection of race to be able to select suitable varieties. If natural contamination race is unknown, biological test is carried out on all the races; 2.2. biological examination consists of tests in pots and Potocek test tube; 2.3. checking the pots: 2.3.1 soil samples placed in pots (two-five litres), and each pod of susceptible varieties in the tuber; 2.3.2. must be at least 10 pots per hectare of the area under examination; 2.3.3. pots can be placed outdoors (spring) or greenhouse (winter) with additional lighting (16 hours a day), maintaining adequate soil moisture; 2.3.4. After about 100 days, when created new tubers, plants are removed from the soil and check the symptoms of potato wart; 2.4. the test tube Potocek: 2.4.1. each soil sample testing using 30 susceptible varieties of tubers with the seed started eyes; 2.4.2. soil samples placed in a conical plastic backing (length 8 cm, diameter at the upper end of the 3-4 cm, bottom up — 3.5-4.5 cm). Affix the backing of tubers under test with a simple top clips or elastic links; 2.4.3. shoots allows you to grow through soil five to seven weeks, according to the release. 2.4.4. any shoots that are grown through soil, cut away in order to stimulate the formation of new shoots; 2.4.5. soil shaken off and tubers with shoots in three to four weeks there in closed plastic boxes; 2.4.6. tubers of potato wart the wart checks; 2.5. all biological tests also include a negative control with non-infected soil and positive control with infected soil.
Minister of agriculture, Minister of finance Spurdziņš o.