Veterinary Requirements That Must Be Followed When Swine And Horse Carcasses Of Trichinosis

Original Language Title: Veterinārās prasības, kas jāievēro, veicot cūku un zirgu liemeņu trihinelozes pārbaudi

Read the untranslated law here: https://www.vestnesis.lv/ta/id/109635

Cabinet of Ministers Regulations No. 383 in Riga, 31 May 2005 (pr. No 32 39) veterinary requirements that must be followed when swine and horse carcasses trichinosis inspection Issued in accordance with article 25 of the law on veterinary medicine 1 and 4 point 1. down the health requirements to be followed in the swine and horse carcasses of trichinosis (hereinafter examination).
2. the requirements of these regulations is monitored and controlled by the food and veterinary service (hereinafter service).
3. to determine the presence of trichinae in pig and horse carcasses, samples from slaughtered in Latvia in domestic pigs and horses taken during health inspections and send the check to the accredited or authorized service laboratory. Samples from wild pigs Hunter, merchant, service officer or veterinarian take immediately after killing the pigs, and sent to an accredited or authorized service laboratory.
4. the proposing of the trichinosis inspection the presence of domestic swine and wild swine meat, use one of these rules 1., 2., 3., 4., 5., or listed in annex 6 of the trichinosis investigation methods, except trihineloskopisk (compression).
5. inspection of horse meat, comply with the following conditions: 5.1. taking at least 10 g sample from the lingual muscle or the jaw muscle or, if the tongue or jaw muscle is the diaphragm, from the place of the transition to the sinewy part. The muscle may not be the connective tissue and fat;
5.2.5 g sample, if you use the aggregate sample digestion method of composition in accordance with the provisions of the 2, 3, 4, 5 and 6. The total weight of each muscle tested digestive liquid must not exceed 100 g, if the use of these provisions 2., 3., 4. and 5. the method referred to in the annex, or 35 g if 6. these provisions use the method referred to in the annex;
5.3. where it is found, in addition to the proposer of the trichinosis take 10 g of the sample and repeat the test. If the horse meat is found in Trichinella, freeze the meat in accordance with one of the rules referred to in annex 9.
6. in Latvia from a third country (a country which is not a Member State of the European Union) may be imported into the skeletal muscles (striated muscles) containing fresh meat (whole carcases, half carcases, quarters or pieces), if the meat is: 6.1 check the supervision of the official veterinarian, using one of the methods of investigation of trichinosis (1., 2., 3., 4., 5., 6., 7.), and it is issued by a veterinary health certificate in accordance with the legislation on veterinary health certificate issued;
UR6.2.mar! with the health mark, as well as a mark to certify that the meat is made of trichinosis inspection (annex 8);
6.3. the split in the European Union in an approved cutting plant.
7. the service may permit the importation from certain third countries or parts thereof of domestic swine, fresh meat which has not undergone the trichinosis inspection, if the European Union approved cutting plant is frozen in accordance with the rules referred to in annex 9 requirements and it is confirmed by the official veterinarian on the meat served in a veterinary health certificate.
Informative reference to European Union directives, the regulations include provisions resulting from: 1) of the Council of 26 June 1964 to Directive 64/433/EEC on health problems affecting intra-Community trade in fresh meat;
2) Council of 21 December 1976 Directive 77/96/EEC on trichinae (Trichinella spiralis) upon importation from third countries of fresh meat of domestic swine;
3) Council of 16 June 1992 Directive 92/45/EEC concerning public health and animal health problems that sais clouded by the killing of wild game and the placing on the market of wild-game meat.
Prime Minister a. Halloween Minister of Agriculture m. Roze Editorial Note: rules shall enter into force on 4 June 2005.
 
1. the annex to Cabinet of Ministers on 31 May 2005 the Regulation No 383 artificial digestion method 1. Apparatus and materials: 1.1. knife for taking samples;
1.2. the small, numbered close to the dish for the storage of the samples (if required repetitive tests);
1.3. incubator;
1.4.2 to 3 litre glass funnel with stand, rubber savienotājšļūten, staples of the savienotājšļūten;
1.5. plastic sieve about 18 cm in diameter with approximately 1 mm perforations;
1.6. gauze;
1.7. a small tapered tube with a sealed end;
1.8. a large glass dish;
UR1.9.ga ļasmašīn with 2 mm perforations;
1.10. stereo-microscope (magnification 15 to 40reiz) with a suitable light source;
1.11. digestive enzyme solution that prepares, one litre of tap water by dissolving 10 g of pepsin (80FIP (International Pharmaceutical Federation) units per gram) and 5 ml HCl (at least 37%).
2. Sampling: 2.1 at least 20 g to be taken from a whole carcase diaphragm instead of the transition to the sinewy part;
2.2. in the absence of diaphragm pillars, taken at least 20 g to be taken from the rib part or the skirt part, the lingual muscle or the jaw muscle abdominal muscles;
UR2.3.ga-pieces taken at least 20 g of skeletal muscle, containing little fat (if possible near to bones or tendons).
3. the method: UR3.1.lai test 10cūk the aggregate, from each individual 20 g sample take 10 g. The remaining 10 g shall retain the additional test, where it may be necessary;
3.2.100 g (10 g from 10paraug) grind the meat mincer and the free sieve, lined with gauze. Sieve inserts a funnel that rubber hose connects to a small tapered tube with a closed end. Funnel filled with digestive liquid, pouring their long funnel side to the test material is completely covered. Test of the digestive solution of about 1:20 to 1:30 p.m.;
3.3. after incubation at 37 20stund 18 and up to 39 ° C, the small tapered tube shall be disconnected and removed. Carefully pour off the liquid, the surface of the tube in the precipitate rinse thoroughly on a plate. Then with a stereomicroscope (increase 20 to 40reiz) check for trichinae (Trichinella spiralis);
3.4. If examination of the aggregate sample have tested positive or doubtful result from each carcase, then take another 20 g sample in accordance with paragraph 2 of this annex. Relevant samples checked individually using the method described above.
Minister of agriculture m. Roze annex 2 Cabinet of 31 May 2005, regulations no 383 aggregate artificial digestion method 1. Apparatus and reagents: 1.1. knife and tweezers for sampling;
UR1.2.ier īc Shredder for meat with 2 – 3 mm perforations;
1.3.3 litre Erlenmeyer flask with a rubber or wool;
1.4.2 litre separating funnel conical;
1.5. common stand with A base, approximately 28cm long with 80 cm long stalks;
1.6. the tripod ring (diameter approximately 10 to 11 cm-);
1.7. the stand clamp with a flat Vice (23 × 40 mm) which can be attached to the stand by dubultuzmav;
1.8. the sieve (external diameter 11 cm, weave hole-size 177mik-ron), fitted with brass or stainless steel mesh weave;
1.9. the funnel (internal diameter of at least 12 cm);
1.10.100 ml glass measuring cylinders;
1.11. a stereo-microscope (magnification 15 to 40reiz) with a suitable light source, or trihineloskop with horizontal table for the compressor and suitable light source;
1.12. a larval counting basin (when using trihineloskop), made from 3 mm thick acrylic plates and meet the following dimensions: 1.12.1. the base of the camera is 180 × 40 mm, and it is divided into squares;
1.12.2. the edge size is 230 × 20 mm. size is ultimately 1.12.3 40 × 20 mm. Base and ends are inserted between the sides, thus creating a camera with two small rokturīš in each end. The base of the upper edge of the 7 to 9 mm on the side and bottom of the frame end. Details of suitable materials, 5.1.1.the with glue;
1.13. number of 9 cm diameter Petri plate (when using a stereo microscope), underside, using a pointed object into 10 × 10 mm, squares;
1.14. number of 10litr waste containers to be used to clean the accessories (e.g. with formol), as well as the plant and the rest for storage if the hydrolysate is positive results;
1.15. concentrated hydrochloric acid (37%);
1.16. the concentration of pepsin 1:10000NF (Us National Formulary) corresponding to 1:12500BP (British Pharmacopoe), as well as 2000FIP (International Pharmaceutical Federation);
1.17. more trays, suitable for the placement of 50paraug (each sample is approximately 2 g);
1.18. the scales to the nearest 0, 1 g.
2. Sampling: 2.1 having approximately 2 g sample from whole carcasses of transitional diaphragm from muscular parts of subparagraph cīpslain;
2.2. If the diaphragm is not, then take the same size to be taken from the rib part or the skirt part of the tongue muscles, the muscles of the jaw or the abdominal muscles;
UR2.3.ga-pieces take about 2 g of the sample of striated muscle, which contains little fat and located as near as possible to the bones or tendons.
3. Method: full sample group 3.1 (integrated 100paraug): 3.1.1.no 100atsevišķ investigation of the samples takes about 1 g of each;
3.1.2. the aggregate sample is ground meat grinding device;
3.1.3. chopped meat shall be placed in the 3litr of the Erlenmeyer flask, add 7 g of pepsin, approximately 2litr in tap water, preheated to about 40 to 41 ° C, and 25ml of concentrated hydrochloric acid. Mixture shall be shaken to dissolve the pepsin. Determined by the solution pH value (it should match the amount of 1.5 to 2);

UR3.1.4.lai digestion take place (or enzymatic hydrolysis), Erlenmeyer flask approximately four hours are incubated at 40 to 41 ° c. Incubation flask shall be regularly shaken at least two times an hour;
3.1.5. the hydrolysate is filtered through the sieve into a conical 2litr separating funnel and left still stand for at least an hour;
3.1.6. measuring cylinder completely around the Valley 45ml fluid and divided by three Petri plates that base divided into squares, i.e. nodes each plate;
3.1.7. each Petri plate carefully examined minutely for Trichinella larvae;
3.1.8. using the larval counting basin, the 45ml split by two larval counting basin and verified with trihineloskop;
3.1.9. the larvae appear as identifiable organisms in the sediment, and in lukewarm water can often be observed in spiral reel and rewind motion;
3.1.10. digestion checks as soon as it is ready. Check in no case should be postponed until the next day;
3.1.11. If digests are not clear or are not checked will adjust after preparation, it clarified the following: 45 ml is poured into a measuring cylinder and the sample stand for 10. After 10 minutes of fluid 30 ml vacuum and the remaining nodes with tap water added to 45ml. Yet after the settling of 10minūš fluid vacuum 30 ml and the remaining nodes poured on the Petri plate or the larval counting basin to check. The measuring cylinder should be washed with 10 ml of tap-water and these washings should be added to the sample in the Petri plate or larval counting basin;
3.2. the investigation of the group, if there are less than 100paraug: 3.2.1 number of samples not more than 15, you can add the aggregate sample, which is 100paraug, and to examine together;
3.2.2. If the test sample is more than 15, but is less than 100, the digestion fluid is reduced proportionately;
3.3. If the results obtained by examination of the aggregate sample is positive or doubtful, from each carcase further 20 g sample should be taken in accordance with paragraph 2 of this annex. 20 g samples from five pig carcasses, combine into one sample and tested according to the method described above. In this way, check the 20kopparaug, each of which combines the five samples of pig carcases. Discovering the Trichinella five pig carcasses, the aggregate of the relevant groups still take 20 g samples from the carcasses to be checked individually, using the method described above.
Minister of agriculture m. Roze annex 3 Cabinet of 31 May 2005, regulations No mechanically facilitate aggregate in 383 digestion method (method of sedimentation) 1. Apparatus and reagents: 1.1. knife or scissors for cutting specimens;
1.2. a tray divided into 50kvadrāto, which may be positioned approximately 2 g of meat samples;
1.3. Lab-blender, Blender model 3500 Thermo;
1.4. stomacher Lab-blender suitable plastic bags;
1.5.2 liter conical separating funnel, preferably with teflon stopper safety;
1.6. the stand, the stand rings and clamps;
1.7. the wire mesh of stainless steel with mesh holes the size of 177mikron and an external diameter of 11 cm;
1.8. the sieve for insertion funnel provided with an internal diameter of at least 12 cm;
1.9.100 ml glass measuring cylinders;
1.10.25 ml dispenser;
1.11.3 liter volumetric beakers;
1.12. spoon or glass rod digestion fluid mixing in a beaker;
UR1.13.ats ūkšan for plastic syringe and tube;
1.14.6 some g scoop;
1.15. the thermometer with an accuracy of 0,5 ° C measuring range from 1līdz ° C; 100 1.16 vibrator, e.g. electric shaver to remove the nozzle;
1.17. the relay that turns on and off with one-minute intervals;
1.18. trihineloskop with a horizontal table or a stereo-microscope, with a suitable light source;
1.19. larval counting basin (when using trihineloskop), made from 3 mm thick acrylic plates and meet the following dimensions: 1.19.1. camera mount is 180 × 40 mm, and it is divided into squares;
1.19.2. edge size is 230 × 20 mm. size is ultimately 1.19.3 40 × 20 mm. Base and ends are inserted between the sides, thus creating a camera with two small rokturīš in each end. The base of the upper edge of the 7 to 9 mm on the side and bottom of the frame end. Details of suitable materials, 5.1.1.the with glue;
a number of 9 cm diameter 1.20 Petri plate (if you use the stereomikr screwy), underside, using a pointed object into 10 × 10 mm, squares;
1.21.17.5% hydrochloric acid solution;
1.22. the concentration of pepsin 1:10 000 NF (Us National Formulary) corresponding to 1:12500 BP (British Pharmacopoe), as well as the 2000 PIP (International Pharmaceutical Federation);
1.23. multiple of 10 litres of waste containers to be used to clean the accessories (e.g. with formol), as well as the plant and the rest for storage if the hydrolysate is positive results;
1.24. balance, accurate to 0.1 g. 2. Sampling: 2.1 having about 2 g of whole carcases a sample from the diaphragm to the transition from muscular parts of subparagraph cīpslain;
2.2. If the diaphragm is not having the same size to be taken from the rib part or the skirt part of the tongue muscles, the muscles of the jaw or the abdominal muscles;
UR2.3.ga-pieces take about 2 g of the sample of striated muscle, which contains little fat and located as near as possible to the bones or tendons.
3. Method: 3.1 digestion method: 3.1.1. full sample groups (combined 100 samples) the investigation carried out in the following order: 3.1.1.1. Lab-blender 3500 Blender put in a double plastic bag and the temperature control set at 40 to 41 ° C;
UR3.1.1.2.iek šēj plastic bag, pour 1 5litr of water preheated to about 32-35 ° C, and the water heated to 40 to 41 ° C;
3.1.1.3. water in the stomacher add 25 ml 17.5% hydrochloric acid;
3.1.1.4. the 100paraug (each about 1 g, temperature 25-30 ° C) taken from each of the individual samples, in accordance with paragraph 2 of this annex;
3.1.1.5.6 g of pepsin is added. Adheres to this order, to prevent decomposition of the pepsin;
3.1.1.6. in processing the contents of the bag in the stomacher 25 minutes;
3.1.1.7. plastic bag is removed from the stomacher and the digestion fluid is filtered through a sieve 3litr beaker;
3.1.1.8. plastic bag is washed with approximately 100 ml of water, which is then used to rinse the sieve, and at the end add the filtrate in a beaker;
3.1.2. the investigation of the group, which is less than 100paraug. If the number of samples not more than 15, you can add a set of model 100paraug and check with them. If the test sample is more than 15, but is less than 100:3.1.2.1. Lab-blender 3500 Blender put in a double plastic bag and the temperature control set at 40 to 41 ° C;
3.1.2.2. prepares the digestion fluid, mixing 1, 5litr with 25 ml water 17.5% hydrochloric acid. 6 g of pepsin is added and mix all together 40 to 41 ° c. Adheres to this order, to prevent decomposition of the pepsin;
3.1.2.3.no the digestion fluid takes a quantity corresponding to 15 ml per gram of sample (for example, 30 samples the volume required is 30 × nodes or 450 mL) and pour into the inner plastic bag, add about 1 g of big meat samples (25 to 30 ° C) taken from each of the individual samples, in accordance with paragraph 2 of this annex;
3.1.2.4. outer bag Valley water temperature is around 40 ° C, so that the total volume of the two bags to be 1, 5litr;
3.1.2.5. in processing the contents of the stomacher bags in 25 minutes;
3.1.2.6. plastic bag is removed from the stomacher and the digestion is filtered through a sieve 3litr beaker;
3.1.2.7. plastic bag is washed with approximately 100 ml of water, which is then used to rinse the sieve and finally add the filtrate in a beaker;
3.2. larval isolation with sedimentation: 3.2.1 the digestion fluid to add 300 to 400 g of ice (flake, powder or crushed), increasing its volume up to about two litres. The digestion fluid is stirred until the ice has melted. Less sample volume (3.1.2. of this annex) ice quantity shall be reduced accordingly;
3.2.2. the chilled digestion fluid is poured into a separating funnel 2litr, special clamps attached to the vibrator;
3.2.3.30 minutes sedimentation takes place, during which the sedimentation funnel alternately subjected to vibrations, i.e. one minute vibration followed by a one-minute break;
3.2.4. after 60 ml of sediment quickly adjust pour 100 ml measuring cylinder. The funnel is rinsed after use with detergent solution;
3.2.5.60 ml sample to stand for at least 10 of the supernatant after settling suction, leaving 15 ml of sediment, which verifies the existence of larvae;
UR3.2.6.ats ūkšan may be used for single use syringe with attached plastic tube. The tube must be so long as to nodes remain in the measuring cylinder, syringe side projections based on the sides of the cylinder;
3.2.7. the remaining nodes is poured into a larval counting basin or two Petri plates and check the use trihineloskop or a stereo;
3.2.8. digestion checks as soon as it is ready. Check in no case should be postponed until the next day;

3.2.9. If digests are not clear or are not checked will adjust after preparation, it clarified the following: 60 ml sample into the measuring cylinder and settle the Valley 10. After 10 minutes of fluid vacuum 45ml and the remaining nodes with tap water up to 45ml. Yet after the settling of 10minūš fluid vacuum 30 ml and the remaining nodes poured on Petri plates or poured into a larval counting basin to check. The measuring cylinder should be washed with 10 ml of tap-water and these washings should be added to the sample in the Petri plate or larval counting basin;
3.3. If the results obtained by examination of the aggregate sample is positive or doubtful, from each pig takes a further 20 g sample in accordance with paragraph 2 of this annex. 20 g samples from five pig carcasses, combine into one sample and tested according to the method described above. In this way, check the 20paraug, each of which combines the five samples of pig carcases. Discovering the Trichinella five pig carcasses, the aggregate of the relevant group of pig carcases still take 20 g samples, which are tested individually using the method described above.
Minister of agriculture m. Roze annex 4 of the Cabinet of Ministers on 31 May 2005 the Regulation No 383 mechanically facilitate the aggregate sample digestion method (method of isolation with the filter) 1. Apparatus and reagents (additional to annex 3 of these regulations referred to in paragraph 1, the following are required equipment): 1.1.1 litre Gelman funnel with filter holder (diameter 45 mm);
1.2. the filter disc, which consists of: UR1.2.1.apa-shaped stainless-steel mesh-mesh with an aperture of 35mikron (disk diameter-45 mm);
1.2.2. the two rubber rings made of 1 mm thick rubber (the external diameter 45 mm and the internal diameter 38 mm). Circular sieve placed between the two rubber rings and bonded to them using glue suitable for the two materials in salīmēšan;
1.3.3 litre Erlenmeyer flask to which side of the suction hose fitted;
1.4. This filter pump;
1.5. a plastic bag with a capacity of not less than 80 ml; UR1.6.ier īc in plastic bag aizkausēšan;
1.7. rennet in the first grade-150000sokslet strength units per gram.
2. Samples shall be taken in accordance with the provisions of paragraph 2 of annex 3.
3. Method: 3.1 digestion method (this rule 3.1 of annex 3);
3.2. larval isolation with filtration: 3.2.1 the digestion fluid to add 300 to 400 g of ice (flake, powder or crushed), increasing its volume to about 2litr. Less number of ice samples quantity shall be reduced accordingly;
3.2.2. the digestion fluid is stirred until the ice has melted. Then allow the solution to cool for at least three minutes to stand for the larvae to be twisted;
3.2.3.uz the Erlenmeyer flask to which you added to the filter pump, Gelman funnel fitted with filter holder and filter disc;
3.2.4. the digestion fluid is poured Gelman funnel and filter. The end solution of filtration through the filter can contribute with suction via the filter pump. Stop the suction filter before become dry, i.e. when the funnel remained 2 to 5 ml of fluid;
UR3.2.5.kad filter out all of the digestion fluid, remove the filter and place the disc 80 ml plastic bag, adding 15 to 20ml rennilase solution. Rennilase solution, 2 g of rennilase adding 100 ml of tap water;
3.2.6. plastic bag sealed and placed twice in the stomacher between the inner and outer bag;
3.2.7. stomacher operated three minutes;
3.2.8. after three minutes, the plastic bags with filter disc and rennilase solution is removed from the stomacher and returns with the scissors. Liquid poured the contents of a larval counting basin or Petri plates. Rinse the bag with 5 to 10 ml of water, add a larval counting basin by content, so check with trihineloskop or poured on the Petri plate to check with a stereo;
3.2.9. digestion checks as soon as it is ready. Check in no case should be postponed until the next day (in no way should use filter discs, which are not completely clean. Dirty disks must not allow to dry. You can clear the filter discs, overnight leaving them in rennilase solution. Before use, wash them in fresh rennilase solution using a stomacher);
3.3. If the results obtained by examination of the aggregate sample is positive or doubtful, yet taken from each pig 20 g of the sample in accordance with the provisions of paragraph 2 of annex 3. 20 g samples from five pig carcasses, combine into one sample and tested according to the method described above. In this way, check the 20paraug, each of which combines the five pig carcases, gi. Discovering the Trichinella five pig carcasses, the aggregate of the relevant group of pig carcases still take 20 g samples, which are tested individually using the method described above.
Minister of agriculture m. Roze annex 5 Cabinet on 31 May 2005 the Regulation No 383 aggregate sample digestion magnetic stirring method 1. Apparatus and reagents: 1.1. knife and tweezers for cutting specimens;
1.2. a tray divided into 50kvadrātveid areas, which can be placed approximately 2 g of meat samples;
1.3. the moulinette Blender;
1.4. magnetic stirrer with thermostatically controlled heating plate, which can be controlled with a thermostat, about 5 cm long, with teflon coated stirring rods;
1.5 tripods, Tripod rings and clamps;
UR1.6.ner ūsējoš steel wire sieve mesh which hole size is 177 microns, external diameter 11 cm 1.7.-hopper with at least 12 cm diameter (sieves for insertion of);
1.8.3 litre beaker;
1.9. measuring cylinders around with 50 ml volume or centrifuge tube;
1.10. trihineloskop with a horizontal table or a stereo-microscope, with a suitable light source;
1.11. larval counting basin (when using trihineloskop), made from 3 mm thick acrylic plates and meet the following dimensions: 1.11.1. camera mount is 180 × 40 mm, and it is divided into squares;
1.11.2. the edge size is 230 × 20 mm. size is ultimately 1.11.3 40 × 20 mm. The bottom and the ends should be inserted between the sides, thus creating two rokturīš on each end. The upper edge of the base must be in the 7 to 9 mm on the side and bottom of the frame end. Details must be attached with adhesive applied to the material;
1.12. a number of 9 cm diameter Petri plate (if you use the stereomikr screwy), underside, using a pointed object into 10 × 10 mm, squares;
1.13. aluminium foil, 1.14.25% hydrochloric acid;
1.15. the concentration of pepsin 1:10000 NF (Us National Formulary) corresponding to 1:12500 BP (British Pharmacopoe), as well as the 2000 PIP (International Pharmaceutical Federation);
UR1.16.kr-Europe-water heated to 46 – 48 ° C;
1.17. more 10litr waste containers to be used to clean the accessories, such as formol, as well as the equipment and the storage of the remaining hydrolysate when positive results are obtained;
1.18. balance, accurate to 0.1 g;
1.19.2 liter conical separating funnel.
2. Sampling: 2.1 having about 2 g of whole carcases a sample from the diaphragm to the transition from muscular parts of subparagraph cīpslain;
2.2. If the diaphragm is not having the same size to be taken from the rib part or the skirt part of the tongue muscles, the muscles of the jaw or the abdominal muscles;
UR2.3.ga-pieces take about 2 g of the sample of striated muscle, which contains little fat and located as near as possible to the bones or tendons.
3. Method: full sample group 3.1 (combined 100 samples) investigation: 3.1.1.100 samples (each about 1 g, taken in accordance with paragraph 2 of this annex) grind the moulinette blender. Blender operates three to four times, each time for about a second;
3.1.2. chopped meat is placed in a beaker and 3litr spray with 10 g of pepsin. 2litr beaker in tap water, preheated to 46-48 ° C, and add 16ml hydrochloric acid;
3.1.3. The moulinette Blender's smalcinātājieliktn tend to immerse the beaker in an existing digestion fluid to remove the remains of meat;
3.1.4. the mixing Rod inserts a beaker, cover with aluminum foil;
3.1.5. the beaker is placed on the previously heated hotplates magnetic stirrer and stirring process started. Before you begin the process of mixing, the magnetic stirrer should be adjusted so that all life could maintain a constant temperature of 44-46 ° c. The stirring process, the digestion fluid should rotate at high speed, enough to create a deep whirl without splashing;
3.1.6. the digestion fluid continue to stir for 30 minutes. Then switch off the mixer and the digestion fluid is poured through a sieve sedimentation funnel;
3.1.7. the digestion fluid is allowed to stand for the funnel adjust;
3.1.8. after adjust of the digestion fluid 40 ml sample is quickly measuring cylinder or centrifuge tube;
3.1.9. the sample (40ml) stand for 10 minutes. After the settling of the purged fluid 30 ml, leaving 10 ml; the remaining 10 ml. 3.1.10 sediment sample is poured into a larval counting basin or Petri plate poured on;
3.1.11. the cylinder or centrifuge tube is rinsed with about 10 ml of tap-water that is added to the sample in the larval counting basin or Petri plates. Then the sample is checked with a trihineloskop or a stereo;

3.1.12. digestion checks as soon as it is ready. Check in no case should be postponed until the next day;
3.1.13. If digests are not examined within 30 minutes after their preparation, it clarified the following: approximately 40 ml of the sample into the measuring cylinder Valley and let it stand for 10 minutes. Then separates from the settling of fluid 30 ml of fluid, leaving 10 ml. This volume with tap water up to 40 ml of fluid after 10 minutes of evacuation, leaving 10 ml 30 ml, which poured on Petri plates or poured into a larval counting basin. The measurement should be washed with 10 ml of tap-water and these washings should be added to the sample in the Petri plate or larval counting basin;
3.1.14. If during an inspection finds that the sludge is not clear, the sample into the measuring cylinder and supplemented by Valley tap water to 40 ml, then repeat the above procedure.
3.2. the investigation of the group, if there are less than 100 samples: 3.2.1. where appropriate, look to the total 100 gu group can add up to 15 samples (each sample – 1 g) and check them together under this annex 3.1;
3.2.2. If the number of samples exceeding the 15 checks as a full sample group. If the sample group is 50 or less, the sample digestion fluid may be reduced to one litre;
3.3. If the results obtained by examination of a collective sample, a positive or questionable carcasses from each pig's still take 20 g in accordance with paragraph 2 of this annex. He yanked the gus, taken from five pig carcases (20 g), combines the total sample and tested according to the method described above. In this way, the test samples from 20 groups, taken from five pigs. Discovering the Trichinella five pig carcasses, the aggregate of the individual pig carcases takes a further 20 g sample should be checked individually by using the method described above.
Minister of agriculture m. Roze annex 6 Cabinet on 31 May 2005 the Regulation No 383 automatic digestion method for pooled sample, weighing not more than 35 g 1. Apparatus and reagents: 1.1. knife or scissors for cutting specimens;
1.2. the tray, divided into 50 square yards, which might be placed approximately 2 g of meat samples;
1.3. Trichomatic 35 blender with filtration;
1.4. hydrochloric acid solution 8.5% ± 0.5 weight,;
1.5. the transparent polycarbonate membrane filters with a 50 mm diameter and 14mikron of large pore size;
1.6. the concentration of pepsin 1:10000 NF (Us National Formulary) corresponding to 1:12500 BP (British Pharmacopoeia), as well as the 2000 PIP (International Pharmaceutical Federation);
1.7. balance, accurate to 0.1 g;
1.8. tweezers with flattened ends;
1.9. number of microscope slide with a side length of at least 5 cm or more of at least 6 cm diameter Petri plates, underside, using a pointed instrument, is divided into 10 × 10 mm large squares;
1.10. microscope with transmitted light (stereo) (15 to 60 times magnification) or a trihineloskop with a horizontal table;
1.11. the tank liquid waste;
1.12. a number of 10 litre bins to be used to clean the accessories (e.g. with formol), as well as the plant and the rest for storage if the hydrolysate obtained positive results.
2. Sampling: 2.1 having about 2 g of whole carcases a sample of the diaphragm in the transition from muscular parts of subparagraph cīpslain;
2.2. If the diaphragm is not having the same size to be taken from the rib part or the diaphragm from the part of the jaw muscle or the abdominal muscles;
UR2.3.ga-pieces take about 2 g of the sample of striated muscle, which contains little fat and located as near as possible to the bones or tendons.
3. Method: 3.1 digestion method: place the mixer in 3.1.1 filtration, waste is added between the led tube, which is transmitted to the container;
3.1.2. If the mixer is switched on, the heating will start;
3.1.3. before you begin, you must open and close the lower valve located below the reaction Chamber;
3.1.4. Add not more than 35, the maritime industries and services (each approximately 1 g, taken in accordance with paragraph 2 of this annex, the temperature 25-30 ° C). Make sure that the major string pieces are removed because they can get stuck in the membrane filters;
3.1.5. pour water up to the edge of the liquid Chamber that is connected to the stirrer (approximately 400 ml);
3.1.6. pour about 30 ml of hydrochloric acid (8.5%) to less fluid camera edges;
3.1.7. place a membrane filter under the coarse filter gasket filter holder;
3.1.8.7 g of pepsin is added. Order must be strictly observed to prevent decomposition of the pepsin;
3.1.9. the concluding response and fluid camera covers;
UR3.1.10.izv the digestion have life-a short enzymatic hydrolysis (5 minutes) the normal age of slaughter pigs and enzymatic hydrolysis of longer duration (8 minutes) the other samples. Automatic spraying begins by pressing the Start button the mixer. After the digestion happens automatically, followed by filtration. After 10 to 13 minutes the process is over (automatically);
3.1.11. the reaction chamber lid open after you have checked whether the camera is empty. If the camera is in the foam or liquid digestion residues, repeat the procedure in accordance with point 3.4 of this annex;
3.2. larval isolation: UR3.2.1.no view filter holder and replace the membrane filter to a slide or a Petri plate;
3.2.2. membrane filter with an overview of the microscope or trihineloskop;
ārt cleaning UR3.3.iek: 3.3.1. If the result is positive, 2/3 Blender reaction chamber filled with boiling water. The connected liquid Chamber in the Valley of the ordinary tap water until the lower level sensor is under water. Automatic cleaning. Cleans the filter holder and the other equipment (for example, by treatment with formalin);
3.3.2. after one day's work in the Valley Chamber of liquid water tumbler and take action according to the standard program;
3.4. use of membrane filters each polycarbonate membrane filter may be used up to five times. Filter cuts off between applications. After each use, the filter checks whether there are any defects that could make them unfit for further use;
3.5. If digestion is incomplete and therefore cannot perform filtering, use another method. After the end of the process (carried out in accordance with this annex, point 3.1) open the lid to the reaction chamber and check if the camera is left in the foam or liquid. In this case, perform the following procedure: 3.5.1 the bottom valve closes before the reaction Chamber;
UR3.5.2.no takes the filter holder and replace the membrane filter to a slide or a Petri plate;
3.5.3. Insert new membrane filter in the filter holder and place the filter holder back;
UR3.5.4.lej Blender liquid Chamber with water until the lower level sensor is under water;
3.5.5. Auto cleaning;
3.5.6. after completion of the cleaning program, open the lid to the reaction chamber and check that there are not still left in the liquid;
3.5.7. If the Chamber is empty, remove the filter holder and transfer the membrane filter with tweezers sets it to a slide or a Petri plate;
3.5.8. membrane filters tested both in accordance with this annex, point 3.2. If the filters cannot be verified, repeat the entire digestion process of enzymatic hydrolysis with longer in accordance with this annex, point 3.1.;
3.6. If the results obtained by examination of the aggregate sample is positive or questionable carcasses from each pig's still take 20 g of the sample in accordance with paragraph 2 of this annex. These samples tested separately according to the above method.
Minister of agriculture m. Roze annex 7 Cabinet on 31 May 2005 the Regulation No 383 Trihineloskopisk (compression) method 1. Apparatus and materials: 1.1. trihineloskop filament lamp (with 50 and 80-100 times magnification) that meets the following criteria: 1.1.1. simple operation;
1.1.2. high light intensity: 1.1.2.1. accurate results are obtained even when the room is completely dark;
UR1.1.2.2.par light source uses 100 W (12 V) projector lamp;
1.1.3. the appropriate magnification: 1.1.3.1. normal working magnification: 50 times;
1.1.3.2.80 – 100 times magnification for more assessment of objects not clearly identifiable under normal working magnification;
1.1.4. capacity-each magnification obtained clear and sharp, well-defined color images;
UR1.1.5.sl ēdž mechanism-after each change of magnification to automatically adjust the brightness of the picture;
1.1.6. increase the contrast: 1.1.6.1. capacitor is IRIS diafrag ma, which makes it possible to increase the contrast of the complex case more thorough examination;
1.1.6.2. iris diaphragm is easy-to-operate (for example, the control lever on the platform of trihineloskop);
1.1.7. easy focusing: 1.1.7.1. fast focusing, using the adjustable ring;
1.1.7.2. precise focusing, using the control lever;
1.1.8. voltage adjustment (sharpness can be adjusted according to your needs);
1.1.9. one-way movement of the compressor – the automatic locking mechanism of secure compressor movement in only one direction, to prevent unintentional displacement;
UR1.1.10.br array to see the projector screen (at least 54 cm in diameter), high capacity reflective, durable and easy to clean;
1.2. the compressor, consisting of two glass plates (one of which is divided into equal squares);
1.3. small curved scissors;
1.4. small surgical forceps;
1.5. the knife cutting of samples;
1.6. the small numbered samples for separate storage jar;
1.7. dropping pipette;

UR1.8.gl fucking a glass of acetic acid and potassium hydroxide solution of relaxation or induration dehydrated meat softening.
2. Sampling: 2.1.no of whole carcases: 2.1.1. take at least one specimen the size of a Hazel nut from both diaphragm pillars at the transition of the muscular parts to the sinewy part;
2.1.2. If there is only one diaphragm, having one sample size of two hazelnuts;
2.1.3. If no diaphragm, two the size of a hazelnut, samples from the rib part or the skirt part, or from the lingual muscle or the jaw muscle or the abdominal muscles;
2.2.no each piece of meat take three samples of skeletal muscle, 2.2.1.no: containing little fat, if possible the size of a hazelnut-;
2.2.2.no various places – where possible near to bones or tendons.
3. Method: preparation of preparation 3.1 trihineloskopij: 3.1.1. If both diaphragm pillars, two specimens are cut from the 7 pieces of oats grain size, i.e. the total of 14 pieces;
3.1.2. If there is only one diaphragm, then cut away the 14 pieces, from different places and if possible from the site, the transition to the sinewy part;
3.1.3. where samples are taken from the rib part or the skirt part, the lingual muscle or the jaw muscle abdominal muscles, then 14 cut oat cereal-size pieces of each model, i.e., a total of 28 pieces;
3.1.4.no each piece of meat cut 4 of the sample size of the OAT cereal pieces, i.e., a total of 12 pieces;
3.2. If the test the meat is dried and old, the preparations prior to the crushing 10-20 minutes to soften the solution consisting of one part potassium hydroxide solution and two parts water;
3.3. all pieces firmly between the compressor compresses the glass plates;
3.4. each preparation is scanned slowly and carefully. If you discover a suspicious area, which is not clearly identifiable, even with the highest increase in its trihineloskop checked by microscope. Microscopic examination is carried out, so that each preparation is scanned slowly and carefully study the magnification of 30 to 40;
3.5. If the result is not clear, the test continues with other models and preparations, if necessary, also with a higher increase, as long as you get the necessary information;
3.6. the verification shall be carried out for at least three minutes. If the case is taken from the rib part or the skirt part, the lingual muscle or the jaw muscle abdominal muscles, the trihineloskopisk test is carried out at least six minutes. The minimum time fixed for the examination does not include the taking of samples and the preparation of the necessary preparation time. Trihineloskopisk in the examiner a day checking up to 840 pieces (in exceptional cases up to 1050 pieces).
Minister of agriculture m. Roze Annex 8 Cabinet on 31 May 2005 the Regulation No 383 verified 1. labelling meat which has undergone the trichinosis inspection, the authorised veterinary surgeon's highlights. Authorised veterinarian is: UR1.1.mar ķēšan instruments for which he may transfer to the professional palīgpers only at time of marking and for the required time;
1.2. this annex referred to in paragraph 5 of the label. Mark the number requested by Assistant staff when it should be used.
2. A label is chubby, and its diameter is 2, 5 cm. The labelling shall bear the following legible information: 2.1 is the letter "T", which is 1 cm long arms and 0 centimeters wide;
UR2.2.zem the letter "T" is one of the following sets of letters – CEE, EEG, EWG, EØF, EOK, EEC, ETY, EHS, EMŰ, EEK, EEB, EGK, Kee, or EGS. Letters are 0, 4 cm high.
3. carcases shall be marked with the dye of the counter or metilvi burn mark on the inside of the thigh.
4. Head marked with metilviolet dyes or hot point 2 of this annex to the requirements laid down in the appropriate mark.
5. you can labelled also with a round label. The label is disposable, made of durable materials, and it meets all hygiene requirements. Kat label ram meat or each of the bays, Nimes. The label has such a clearly legible information: 5.1 is the letter "T";
UR5.2.zem the letter "T" is one of the following sets of letters – CEE, EEG, EWG, EØF, EOK, EEC, ETY, EHS, EMŰ, EEK, EEB, EGK, Kee, or EGS. 0.2 cm high letters.
6.Uz packaging is clearly visible label with legible markings according to paragraph 2 of this annex and the health mark.
Minister of agriculture m. Roze 9. Cabinet of Ministers of 31 May 2005 of the Regulation No 383 meat refrigeration techniques 1. First method: UR1.1.ga fo that is already frozen, store, freeze;
1.2. the refrigeration room equipment and the energy supply is to ensure that šināt this annex referred to in point 1.6. temperature reaches very quickly and maintained throughout the room and around the meat;
1.3. insulating packaging before freezing, except for meat is removed prior to insertion into the freezer is frozen in this annex 1.6. temperature referred to in subparagraph;
1.4. individual consignments in the refrigeration room kept separately and turned on;
UR1.5.re ģistr on each insertion date and time of the refrigerating room;
1.6. the freezer is at least minus 25 ° C temperature. Temperature is measured with calibrated thermoelectric instruments and continuously recorded. It can not be measured directly in the cold air flow. Tools stored in a closed place. The tables include the relevant numbers from the meat inspection register, such as the date and time of the commencement and completion of freezing. The table stores year after they dial;
UR1.7.ga fo with a diameter or thickness of up to 25 cm, continuously frozen for at least 240 hours, and meat with a diameter or thickness of between 25 and 50 cm, continuously frozen for at least 480 hours. This freezing process may not be applied to meat which has a larger diameter or is thicker. Refrigeration time calculated from the moment when the freezer has reached to this annex referred to in point 1.6 of the temperature.
2. the second method. Take this annex 1.1., 1.2., 1.3, 1.4 and 1.5. transactions referred to below as well: UR2.1.ga fo with a diameter or thickness of up to 15 cm, frozen, subject to the following conditions: 2.1.1.20 day-minus 15 ° C;
2.1.2.10 days – minus 23 ° C;
2.1.3.6 the day-minus 29 ° C;
UR2.2.ga fo with a diameter or thickness of between 15 cm and 50 cm, frozen, subject to the following conditions:-the day minus 2.2.1.30 15 ° C;
2.2.2.20 day-minus 25 ° C;
2.2.3.12 day-minus 29 ° C;
2.3. the temperature in the freezer must not be higher than the selected inactivation temperature. Temperature is measured with calibrated thermoelectric instruments and continuously recorded. It can not be measured directly in the cold air flow. Tools stored in a closed place. The tables include the relevant numbers from the meat inspection register, such as the date and time of the commencement and completion of freezing. Table stores the year after they are placed.
3. the third way: UR3.1.ga fo frozen, subject to the following conditions: 3.1.1.106 hours-minus 18 ° C;
3.1.2.82 hours-minus 21 ° C;
3.1.3.63 hours, minus 23.5 ° C;
3.1.4.48 hours, minus the 26 ° C;
3.1.5.35 hours-minus 29 ° C;
3.1.6.22 hours, minus 32 ° C;
3.1.7.8 hours-minus 35 ° C;
3.1.8. hour – minus 37 ° C;
UR3.2.ga fo that is already frozen, store, freeze;
3.3. individual consignments in the refrigeration room kept separately and turned on;
UR3.4.re ģistr on each insertion date and time of the refrigerating room;
3.5. freezing room facilities and energy supply are such as to ensure that this annex referred to in point 3.1., the temperature reached very rapidly and maintained throughout the room and around the meat;
3.6. temperatures measured with calibrated thermoelectric instruments and continuously recorded. Thermometer probe inserted into the calibrated piece of meat in the Middle, which is not less than the thickest piece of refrigeration. This calibrated piece of meat placed in the worst case the refrigeration room, but not cooling equipment in the vicinity or directly in the cold air flow. Tools stored in a closed place. The tables include the relevant numbers from the meat inspection register, such as the date and time of the commencement and completion of freezing. Table stores the year after they are placed.
Minister of agriculture m. rose