MINISTER FOR AGRICULTURE Having regard to Law 18 June 1931 n. 987, containing provisions for the protection of crops and agricultural products from enemy causes and related services, as amended; Having regard to Regulation for the application of the law, approved by Royal Decree of 12 October 1933, n. 1700, as amended by Royal Decree of December 2, 1937, n. 2504; Given the EEC Directive of the Council no. 77/93 / EEC of 21 December 1976 on protective measures against the introduction into Member States of organisms harmful to plants or plant products, as amended; Having regard to Legislative Decree 30 December 1992, n. 536, concerning the implementation of Council Directive no. 91/683 / EEC of 19 December 1991 on protective measures against the introduction into Member States of organisms harmful to plants and plant products; Given the Ministerial Decree of 31 January 1996, published in the ordinary supplement no. 33 to the Official Gazette no. 41 of 19 February 1996 on protective measures against the introduction and spread in the territory of the Italian Republic of organisms harmful to plants or plant products; Having regard to Legislative Decree 4 June 1997, n. 143, entitled "Contribution to the regions of administrative functions in the field of agriculture and fisheries, and central administration reorganization"; Given the Ministerial Decree of 6 March 1996, transposing Commission Directives No. 95/65 / EC and n. 95/66 / EC of 14 December 1995 concerning the amendments to the Annexes to the Ministerial Decree of 31 January 1996 on the protective measures against the introduction and spread in the territory of the Italian Republic of pests of plants and plant products; Given the Ministerial Decree of 19 February 1997, transposing Commission Directive n. 96/78 / EC of 6 December 1996 on amendments to the annexes to Ministerial Decree 31 January 1996 concerning protective measures against the introduction and spread in the territory of the Italian Republic of pests of plants and plant products; Given the Ministerial Decree of 27 November 1997 transposing Commission Directives No. 96/14 / EC of 12 March 1996, n. 96/15 / EC of 14 March 1996, n. 96/76 / EC of 29 November 1996 and No. 97/14 / EC of 21 March 1997 which amend certain annexes to Directive n. 77/93 / EEC as well as' the Directive n. 92/76 / EEC recognizing protected zones exposed to particular plant health risks in the Community '; Having regard to the Commission Directive n. 97/46 / EC of 25 July 1997 amending Directive No. 95/44 / EC laying down the conditions under which certain harmful organisms, plants, plant products and other objects listed in Annexes I, II, III, IV and V of the Directive. 77/93 / EEC may be introduced or transferred from one place to another in the Community 'or certain protected zones thereof, for trial or scientific purposes and for work on varietal selections; Given the need 'to implement the Commission Directive n. 97/46 / EC of 25 July 1997 cited above; Under Article. 20, paragraph 1, of the law 16 April 1987 n. 183; Decrees: Art. 1. The annexes to Ministerial Decree 31 January 1996 are amended as follows: 1) Annex XVII, Part A and 'added the following: Section IV: stoloniferous species of plant or tuber of Solanum L. or their hybrids, intended for planting. 1. The material shall be subjected, as appropriate, to appropriate therapy procedures as laid down in technical guidelines FAO / IPGRI. 2. After the treatment referred to in point 1, each unit 'of plant material and' subjected to indexing. All plant material, including indexing plants, is stored on the premises approved, in the quarantine conditions laid down in Annex 1. During the period of indexing the plant material to be officially put into circulation it must be kept in conditions conducive to the normal vegetative cycle undergone visual inspection for any signs or symptoms of harmful organisms including all relevant harmful organisms listed in Directive n. 77/93 / EEC and potato yellow vein disease, on arrival and at regular intervals until senescence. 3. The indexing procedures referred to in point 2 shall follow the technical provisions set out in point 5, in order to detect at least the following harmful organisms: Bacteria: a) Clavibacter michiganensis (Smith) Davis et al. ssp. sepedonicus (Spieckermann and Kotthoff) Davis et al., b) Pseudomonas solanacearum (Smith) Smith. Viruses and viroids: a) Andean potato latent virus, b) Potato black ringspot virus, c) Potato spindle tuber viroid, d) Potato yellowing alphamovirus, e) Potato virus T, f) Andean potato mottle virus, g) Common Virus Potato , A, M, S, V, X and Y (including Yo, Yn and Yc) and potato leaf roll virus. In the case of true seed of potato, however, the indexing procedures shall be carried out in order to detect at least the viruses and virus-like organisms referred to in paragraphs a) to e). 4. The plant material subjected to the visual inspections referred to in point 2 and on which were observed signs and symptoms of harmful organisms subject to an investigation and, where appropriate, an examination designed to determine with greater accuracy possible, the identity 'of the harmful organisms causing the signs and symptoms. 5. The technical provisions referred to in point 3 are as follows: Bacteria: 5.1. For tubers, test the heel end of each tuber. The standard sample and '200 tubers, but the procedure may' also be used for samples with less than 200 tubers. 5.2. For young plants and cuttings, including micro, test the lower sections of the stem and, where appropriate, the roots of each unit 'of plant material. 5.3. It is recommended that testing of progeny tubers, or for species not tuber, of stem bases during normal vegetative growth after the testing referred to cycle 5.1 and 5.2. 5.4. For the material referred to in 5.1, the method for the detection of Clavibacter michiganensis (Smith) Davis et al. ssp. Sepedonicus (Spieckermann and Kotthof) Davis et al. and 'the Community method set out in Annex I to Directive n. 93/85 / EEC. For the material referred to in paragraph 5.2, it can 'be applied this method. 5.5. For the material referred to in 5.1, the method for the detection of Pseudomonas solanacearum (Smith) Smith and 'the interim test scheme in the Annex to the decision that the Commission must take in order to replace the quarantine procedure No. 26 for Pseudomonas solanacearum, as provided by the Organization the European and Mediterranean Plant Protection (EPPO). For the material referred to in paragraph 5.2 it may 'be applied thereto. Viruses and Viroids other than potato spindle tuber viroid. 5.6. The minimum testing for vegetative material (tubers, young plants and cuttings, including micro-plants) shall include a serological test done at or near 'flowering for each of the specified list of harmful organisms other than potato spindle tuber viroid, and followed by an examination biological material that was negative in the serological test. For potato leaf roll virus, two serological tests shall be done. 5.7. The minimum testing for true seed shall be a serological test or a biological test if the first is unavailable. It is strongly recommended to re-submit to an examination a certain percentage of samples that were negative and to use another method to the suspicion. 5.8. The serological tests referred to in paragraphs 5.6 and 5.7 shall be done on glasshouse grown plants, from which samples were taken in at least two points on every stem, including a young fully formed leaves of each stem and a more 'old leaf located approximately in the middle '; We shall be sampled each stem the possibility 'non-systemic infection. For the serological testing, no bulking of leaflets from separate plants, except when the bulking rate has been validated for the method in question; leaflets from each stem may however be bulked to make up the sample from each plant. In the biological examination if you can put together up to five plants with inoculation of a minimum of duplicate indicator plants. 5.9. The appropriate indicator plants to be used for the biological testing referred to in points 5.6 and 5.7 are those listed by EPPO or other officially approved indicator plants that have been shown to detect the viruses. 6. Only material which and 'was directly examined can' be released from quarantine. Where it has been made an indexing of the eye, you can 'be released from quarantine only the progeny of the tested eye. The tuber can not 'be put into circulation because of possible problems with non-systemic infection. Potato spindle tuber viroid. 6.1. Throughout the plant material shall be tested on glasshouse grown plants, as soon as they reached the height of flowering and pollen production. Testing on tuber sprouts / in vitro plants / small seedlings and 'regarded as a preliminary test. 6.2. Samples are taken from a fully expanded leaflet of each stem of the plant. 6.3. All material for testing shall be grown at temperatures above 18 C and preferably above 20 C and with at least a 16 hours. 6.4. The examination is carried out with cDNA or RNA probes labeled radioactively or less, with the return-PAGE (with silver staining) or RT-PCR. 6.5. For probes and return-PAGE method, the maximum composition ratio of the sample and '5. Use of this or higher bulking rates must be validated.