The Minister of agricultural food and forestry policies having regard to Directive 2000/29/EC #, May 8, 2000, on protective measures against the introduction into the community of organisms harmful to plants or plant products and against their spread within the community, as amended; Having regard to the Legislative Decree of August 19, 2005, n. 214, concerning the implementation of Directive 2002/89/EC on protective measures against the introduction into and the spread within the community of organisms harmful to plants or plant products, as amended; Having regard to Council directive no 2008/61/EC of June 17, 2008, establishing the conditions under which certain harmful organisms, plants, plant products and other objects listed in annexes I, II, III, IV and V to Directive 2000/29/EC may be introduced or transferred from one place to another in the community or certain protected zones thereof for trial or scientific purposes and for work on varietal selections; Given the need to transpose the directive. 2008/61/EC, pursuant to art. 57 of Legislative Decree No 214/05; Acquired the national plant protection Committee opinion expressed at its meeting of 27 and May 28, 2010; Acquired the opinion of the Standing Conference for the relations between the State, the regions and the autonomous provinces of Trento and Bolzano, expressed at its meeting of October 7, 2010;
Decrees: article 1. Annexes XVI and XVII of the Legislative Decree of August 19, 2005, # 214, I'm so modified below: a) in annex XVI, the words ' Directive 95/44/EC ' shall be replaced by the words ' Directive 2008/61/EC ';
b) in annex XVII, part A, section II, the words ' Council Directive 77/93/EEC ' are replaced by ' Council Directive 2000/29/EC ';
c) in annex XVII, part A, it is added to the following section IV: section IV: plants of stolon-or tuber-forming species of Solanum l. or their hybrids, intended for planting 1. The plant material must be submitted, as appropriate, to appropriate therapy procedures as laid down in FAO/IPGRI technical guidelines. 2. After the treatments referred to in point 1, each unit of the plant material is subject to indexing. All plant material including indexing plants, shall be kept in approved plants, in the quarantine containment conditions laid down in annex I. During the time of indexing, the plant material to be entered officially in circulation should be stored under conditions which promote normal vegetative cycle and subjected to Visual inspection for signs or symptoms of harmful organisms including all relevant harmful organisms listed in Directive 2000/29/EC and potato yellow vein disease, on arrival and subsequently, at regular intervals until senescence. 3. indexing procedures referred to in point 2 should follow the technical provisions set out in point 5, in order to detect at least the following harmful organisms: bacteria: a) Clavibacter michiganensis (Smith) Davis et al. SSP. sepedonicus (Spieckermann et Kotthoff) Davis et al.;
b) Ralstonia solanacearum (Smith) Yabuuchi et al., viruses and viroids: a) andean potato latent virus;
b) potato black ringspot virus;
c) potato spindle tuber viroid;
d) potato yellowing alfamovirus;
e) potato virus T;
f) andean potato mottle virus;
g) common viruses, A, M, S, V, X and Y (including Yo, Yn and Yc) and potato leaf roll virus. In the case of true seed potato tubers, however, indexing procedures must be carried out so as to locate at least viruses and virus-like organisms referred to in points a) to e). 4. The plant material subjected to the Visual inspections referred to in point 2 and on which signs and symptoms of harmful organisms have been observed shall be the subject of an investigation and, where appropriate, of an examination to determine with greater accuracy as possible, the identity of the harmful organisms causing the signs and symptoms. 5. The technical provisions referred to in paragraph 3 are as follows: for bacteria: 1) to the tubers, examine the navel of each tuber. The standard sample size is 200 tubers, but the process can also be used for samples of less than 200 tubers;
2) for plants and cuttings, including micro-plants, look in the lower parts of the stem and, if necessary, the roots of each unit of the plant material;
3) it is recommended to examine the progeny tubers, or, for those species not tuber-forming species, the base of the shaft during normal growing period following the examination referred to in paragraphs 1 and 2;
4) for the material referred to in point 1, the method for the detection of Clavibacter michiganensis (Smith) Davis et al. SSP. Sepedonicus (Spieckermann et Kotthof) Davis et al. It is the Community method set out in annex I to Directive 93/85/EEC (1). For the material referred to in point 2, can be applied that method;
5) for the material referred to in point 1, the method for the detection of Ralstonia solanacearum (Smith) Yabuuchi et al. It's the pattern of examination in annex II to Directive 98/57/EC (2). For the material referred to in point 2 can be applied that method;
for viruses and viroids other than potato spindle tuber viroid: 1) minimum examination for vegetative material (tubers, seedlings and cuttings, including micro-plants) consists of a serological test done at or near flowering for each of the harmful organisms other than potato spindle tuber viroid specifically listed, followed by a biological test of material that has given a negative result to the serological test. For binding virus must be carried out, two serological tests;
2) minimum examination for true seed consists of a serological test or a biological examination, if the first is not available. You are strongly advised to refer back to a certain percentage of samples fail and to use another method for borderline cases;

3) serological tests referred to in items 1 and 2 organic and are to be done on glasshouse grown plants, from which samples were taken in at least two places of each stem, including a young leaf fully formed at the apex of each stem and a leaf older situated about halfway; You should take samples from each stem, having regard to the possibility of systemic infections. For serological examination must not be put together the leaves of different plants, except if the ratio of sample composition has been validated for this method; the leaves of each stem can be grouped to constitute the sample of a single vegetable. In the case of biological examination you can put together up to five plants by inoculating a minimum number of indicator plants;
4) plants indicators to be used for biological examination referred to in paragraphs 1 and 2 are those listed by the European and Mediterranean plant protection Organization (EPPO), or other officially approved indicator plants that have been shown to detect any viruses;
5) only material that has been directly examined can date from quarantine. If it has been done with an indexing of the eyes, can date from quarantine only the progeny of the eye examined. The tuber can not be put into circulation due to possible problems with non-systemic infection;
Potato spindle tuber viroid: 1) for all the plant material must be examinations on crops in greenhouses, as soon as they reached the full development, but before flowering and pollen production. Exams on shoots of in vitro grown plants/small tubers/seedlings and is considered only as a preliminary examination;
2) samples are taken from a few perfectly formed at the apex of each stem of the plant;
3) all materials to be examined are planted with temperature no lower than 18° C and preferably more than 20° C and exposure to light at least 16 hours;
4) cDNA or RNA probes the examination takes place with radioactively or not, return-PAGE method (Silver staining) or RT-PCR;
5) for probes and return-PAGE method the maximum sample composition and ratio of 5. The use of this report or of a higher gear must be validated. This Decree will be submitted to the Court of Auditors for registration and will be published in the official journal of the Italian Republic.
Rome, October 19, 2010 Minister: Galan Recorded the Court of Auditors on November 16, 2010, supervising office acts ministries of productive activities, register no. 4, sheet # 338.