Regulation On The Recycling Of Bio-Waste Soils Used In Agricultural, Forestry And Horticultural

Original Language Title: Verordnung über die Verwertung von Bioabfällen auf landwirtschaftlich, forstwirtschaftlich und gärtnerisch genutzten Böden

Read the untranslated law here: http://www.gesetze-im-internet.de/bioabfv/BJNR295500998.html

Regulation on the recycling of bio-waste used in agricultural, forestry and horticultural soils (organic waste regulation - BioAbfV) BioAbfV Ausfertigung date: 21.09.1998 full quotation: "organic waste regulation as amended by the notice of 4 April 2013 (Gazette I p. 658), most recently by article 5 of the Decree of 5 December 2013 (BGBl. I S. 4043) is changed" stand: Neugefasst by BEK. v. 4.4.2013 I 658;
 
Last modified by article 5 V v. 5.12.2013 4043 for more information on the stand number you find in the menu under notes the obligations arising from the Directive 98/34/EC of the European Parliament and of the Council of 22 June 1998 establishing an information procedure in the field of technical standards and regulations and of the rules for the services of the information society (OJ L 204 of the 21.7.1998, p. 37), most recently by Directive 2006/96/EC (OJ L 363 of 20.12.2006, p. 81) is has been modified to comply.
Footnote (+++ text detection from: 1.10.1998 +++) (+++ official note of the standard authority on EC law: respecting the EGRL of 98 at the 34 (CELEX Nr: 31998 L 0034) cf. BEK. v. 4.4.2013 I 658 +++) § 1 scope of application (1) this Regulation applies to 1 untreated and treated bio-waste and mixtures, which are applied or issued for the purpose of applying for recycling as fertiliser on agricultural, forestry or horticultural use floors, and 2. the treatment and study of bio-waste and mixtures.
(2) this Regulation applies to 1 public waste disposal authority and third parties, associations or self-governance bodies of the economy, which according to article 16, paragraph 2, article 17, paragraph 3 or article 18 paragraph 2 of the recycling and waste management act of 27 September 1994 (BGBl. I S. 2705), most recently by article 5 of the law of 6 October 2011 (BGBl. I p. 1986) has been changed, obligations to the recycling of organic waste (waste disposal authority) have been transferred , 2. producers or owners of organic waste or mixtures, as far as they leave these wastes not a waste disposal authority, 2a.
who collects organic waste and transported (collector), 3 those of organic waste treated (Bioabfallbehandler), 4. manufacturer of mixtures by using bio-waste (mixture manufacturers), 4a.
who adopts organic waste or mixtures to the application and gives them without further modification (intermediate pickup) as well as 5 managers of agricultural, horticultural or forestry used soils, where untreated or treated bio-waste or mixtures are to be applied or applied.
(3) this Regulation shall not 1 for home, commercial and small gardens, 2. for own recovery of bio-waste vegetable origin in farms or holdings of gardening and landscaping, if recovery ensured in accordance with articles 6 to 8 on self managed operating surfaces, 3. as far as the sewage sludge Ordinance applies, 3a.
for animal by-products, which according to Regulation (EC) no 1069/2009 of the European Parliament and of the Council of 21 October 2009 laying hygiene requirements for animal by-products not intended for human consumption and repealing Regulation (EC) No. 1774/2002 (animal by-products regulation) (OJ L 300 from November 14, 2009, p. 1), by the directive 2010/63/EC (OJ L 276 of 10, p. 33) has been modified, in its up-to-date version, according to the legislative acts of the European Union, issued for their implementation after the animal by-products disposal Act of January 25, 2004 (BGBl. I p. 82), most recently by article 2 paragraph 91 of the law of 22 December 2011 (BGBl. I S. 3044) changed, amended or the regulations because of the animal by-products disposal Act adopted pursuant to pick up , to collect, to transport, to store, to handle, to process, to use, to eliminate or to bring in traffic, or must be 4. for substances which are disposed of in accordance with other legal provisions.
(4) the rules of the fertilizers and plant protection law remain unaffected. Are bio-waste and animal by-products within the meaning of paragraph 3 number 3a together treated or used in the manufacture of the mixture and applied on soils, the requirements of this Regulation as well as in paragraph 3 provisions referred to apply number 3a.
(5) those referred to in paragraph 2. work towards that the pollutant levels for untreated and treated bio-waste and mixtures referred to in this Regulation as far as possible be fallen below. General cultivation restrictions or other restrictions not referred to in this regulation can not derive from reaching or exceeding the land values pursuant to article 9, paragraph 2.

Article 2 definitions for the purposes of this regulation mean the 1st bio-waste: waste of animal or vegetable origin or fungal materials for recycling, which can be degraded by microorganisms, soil-borne organisms or enzymes including waste for recovery with high organic content of animal or vegetable origin or fungal materials; the bio-waste in particular in annex include 1 number 1 waste referred to in column 1, further upstream in column 2 and closer marked by the supplemental rules in column 3; Floor material does not belong without significant shares of bio-waste to the bio-waste; Residues, incurred on forestry or agricultural and remain in these areas, are not organic waste;
2. sanitiser treatment: biotechnological processing of biodegradable materials for the purpose of sanitizing with a) pasteurisation in accordance with Annex 2 point 2.2.1, b) aerobic hygienisierende treatment in accordance with Annex 2 point 2.2.2 (thermophilic composting), c) anaerobic hygienisierende treatment in accordance with Annex 2 number 2.2.3 (thermophilous fermentation) or d) other hygienisierende treatment in accordance with Annex 2 number 2.2.4;
2A. stabilizing biological treatment: biotechnological processing of biodegradable materials for the purpose of biological degradation of organic matter under aerobic conditions (composting) or anaerobic conditions (fermentation) or other measures for biological stabilization of organic matter; a hygienisierende treatment is 2 letter b or c a biologically stabilizing treatment; after number at the same time
3. untreated organic wastes: bio-waste subjected to any treatment;
4. treated bio-waste: organic waste, which were subjected to a hygienisierenden and stabilizing biological treatment, including in annex 1 number 2 in column 1 is named, in column 2 further defined and complementary provisions in column 2 or column 3 closer marked with treated waste so-called and the supplementary provisions in column 3 closer marked with treated biodegradable materials;
5. mixtures: Mixture of treated and in accordance with § 10 untreated, hygienisierend or stabilizing biologically treated bio-waste with each other and with Annex 1 number 2 in column 1 above, further upstream in column 2 and closer marked by the supplemental rules in column 3 waste or biodegradable materials listed in column 2 and closer marked by the supplemental rules in column 3 and mineral materials, as well as the mixture of a mixture made of above mentioned components with lime in the context of the application. Interbreeding within a common hygienisierenden or biologically stabilizing treatment of bio-waste with each other and with Annex 1 number 2 mentioned materials is no mixture;
6 own recovery: application of plant bio-waste incurred even managed operating surfaces even managed operating areas. (Also the application of a deemed capital recovery) fallen in horticultural services on foreign land plant organic waste on self managed operational areas of the service, b) pro rata repossessed untreated plant bio-waste by members for producer combinations of wine, fruit and vegetable cultivation on self managed operational areas, as far as the vegetal raw materials on operational areas by members of the respective producer associations were produced.

§ 3 handling the hygienisierende (1) waste managers, producers and owners have, if not by an exemption under § 10 paragraph 1 or paragraph 2, recycle organic wastes of boarding or the manufacture of mixtures of hygienisierenden treatment, which the seuchen-and phytohygienische safety guarantees.
(2) the seuchen - and phytohygienische safety is given pursuant to paragraph 1 if any adverse effect on the health of humans or animals due to release or transmission of pathogens and no damage to plants, plant products or floors by the spread of harmful organisms to get. Hygienisierende treatment and the materials to be observed in the individual requirements are laid down in annex 2.
(3) the Bioabfallbehandler has the hygienisierende treatment of bio-waste according to the specifications laid down in annex 2 perform after treatment and the levy or applied to self managed operational areas to ensure the seuchen - and phytohygienische safety of bio-waste. The competent authority may in agreement with the agricultural and veterinary professional competent in aerobic or anaerober hygienisierender treatment of bio-waste in plants with an annual capacity of up to 3 000 tonnes of material exceptions which in paragraph 4 sentence 1(1) and annex 2 contained allow requests to process examination in an individual case. Prerequisite for this is that compensatory measures ensures the seuchen - and phytohygienische safety or no impairment diseases according to type, composition and origin of organic waste - and is expected phytohygienischer concerns. The competent authority may in agreement with the competent authority of agricultural and veterinary specialist other hygienisierende treatment according to § 2 No. 2 admit letter d in individual cases if an equivalent efficacy of sanitizing appropriate to the requirements of annex 2. According to other specifications treated bio-waste considered otherwise hygienisierend treated pursuant to section 2 paragraph 2 letter d, as far as this other way of treating organic waste in annex 1, point 1 column 3 with a reference to this record is listed.
(4) the Bioabfallbehandler has, unless by an exemption under § 10 paragraph 1 or paragraph 2, studies in accordance with paragraphs 5 to 9, perform to let the effectiveness of Hygienisierungsverfahrens by an examination of the process, deviating in pasteurization equipment by a technical inspection on 1, 2. adherence to the required temperature for the necessary duration during the hygienisierenden treatment by process monitoring, and 3 that the maximum permissible limits for pathogens , viable seeds and shoots-capable parts of plants after the hygienisierenden the duty-finished material by testing the sanitized bio-waste treatment.
For examination, they are 4 set methods to apply 2 number in annex.
(5) the Bioabfallbehandler the process audit in accordance with paragraph 4 has set according to the specifications of annex 2 point 3.1 1 number 1 within 12 months after commissioning of a newly built treatment plant to the sanitizing to make. This applies accordingly to already tested systems when using new procedures or significant technical modification of the procedure or litigation. At newly established pasteurization plants, the Bioabfallbehandler instead of the process before has carried out a technical inspection according to the specifications of annex 2 number 2.2.1.2 by the authority responsible for the plant, issuing an inspection certificate about this. The requirements for the conduct and the process audit in coordination with the authorities responsible for the system are set at newly-built facilities for other hygienisierenden before performing the process audit. Up to the successful conclusion of the process audit of Bioabfallbehandler may give the competent authority for recycling materials from the treatment plant to the sanitation with the consent, if the requirements of the process monitoring in accordance with paragraph 4 sentence 1 number 2 and the tests of sanitized organic waste in accordance with paragraph 4 set are met 1 number 3 and are no evidence that contradicts the hygienic safety of these materials.
(6) the Bioabfallbehandler process monitoring referred to in paragraph 4 has set perform point 3.2 to 1 number 2 according to the specifications of annex 2, keeping the following records: 1 hygienisierender at pasteurization temperature history, 2. During Aerobic treatment (thermophilic composting) of the temperature gradient and the implementation dates, 3rd in anaerobic hygienisierender treatment (thermophilous fermentation) on the temperature gradient and the feeding and sampling intervals , 4 in other hygienisierender treatment over the procedure specific parameters set in agreement with the competent authority.
The temperature gradient is to capture with a permanent and interference-free direct temperature measurement in the material to be treated and automated temperature recording during the hygienisierenden treatment. Instead of direct temperature measurement, the competent authority in closed aerobic hygienisierender treatment may admit that the treatment temperature in the exhaust air stream of the compost material is determined. By way of derogation from set 2 the competent authority in open aerobic hygienisierender treatment allow, that the treatment temperature on a regular basis, is measured at least once per working day, and documented. Devices for measuring the temperature need to be calibrated regularly, at least once per year; the calibration shall be documented. He has Bioabfallbehandler the process monitoring determines that the requirements of the litigation were not respected, the competent authority thereof and of the measures taken to inform immediately. The competent authority arranges measures to remain inadequately hygienisierend treated bio-waste as well as the deficiencies if the measures initiated by the Bioabfallbehandler are inadequate or inappropriate.
(7) the Bioabfallbehandler has the tests of sanitized organic waste in accordance with paragraph 4 sentence 1 No. 3 per prisoner to 2 000 tonnes of matter within the framework of the hygienisierenden used organic waste treatment including in annex according to the specifications of annex 2 point 3.3 1 number of 2 of materials to have. The competent authority may in agreement with the competent authority of agricultural trade allow that tests the sanitized organic waste be performed starting at a quantity of more than 2 000 tonnes, the composition used according to type, composition and origin organic waste not or hardly changed. The competent authority can quality or origin of the used bio-waste order significantly changing composition style, that checks the sanitized bio-waste for quantities of less than 2 000 tonnes are performed. Without prejudice to the sentences 1 to 3, the Bioabfallbehandler has a test of sanitized organic waste at a distance of no more than three months. The Bioabfallbehandler has be the sanitized bio-waste a review the limit values pursuant to annex 2 number 4.2.2 or 4.3.2 exceeded, immediately to inform the competent authority of the result of the investigation and the measures taken. The repetition of the exam leads to the same result if repeatedly in different samples analyzed, the limits are exceeded, the competent authority orders measures to remedy the defects.
(7a) by way of derogation from paragraph 7 can set 1 Bioabfallbehandler year more than 24 000 tonnes of matter organic waste including in annex 1 number of 2 called materials treat and are exempt according to § 11 paragraph 3 sentence 1 the presentation of research results or from proof obligations, once a month performed the tests of the sanitized bio-waste. Paragraph 7 sentence 2 to 6 shall apply accordingly.
(8) the investigation when assessing process pursuant to paragraph 4 sentence 1 number 1 and during the tests of the sanitized bio-waste pursuant to paragraph 4 sentence 1 number 3 by independent, perform certain investigative bodies by the competent authority. The Bioabfallbehandler has to submit the investigation results within four weeks after completing the investigation of the competent authority and to store ten years. Records of the process monitoring and the documentation related to the calibration of the thermometers according to paragraph 6 has three years to save Bioabfallbehandler and to submit to the competent authority upon request. Exceeded the limits for pathogens, viable seeds and shoots-capable parts of plants is found when examining the sanitized bio-waste, the findings of the investigating body shall be immediately on the Bioabfallbehandler, which forwards them to the competent authority without delay. This immediately forwards the investigation results to the competent authority of agricultural and veterinary specialist.
(8a) an investigative body is set 1 after paragraph 8 to determine if the applicant has the required expertise, independence, reliability and technical facilities and submit the required documentation. The determination is carried out by the competent authority of the country in which the applicant has his place of business, and is valid for the entire Federal territory; no place of business in Germany, the country is responsible, in which the activity is to be exercised primarily pursuant to paragraph 4. The provision can be equipped with a proviso of the cancellation, a time limit, with conditions, the reservation conditions and requirements. The competent authority may require a nationally active applicant that he had a valid accreditation of compliance with the requirements of DIN EN ISO/IEC 17025:2005 (to refer at the Beuth-Verlag GmbH, 10772 Berlin, and archive terms secured laid down in the German national library in Leipzig) shall submit, refers to the parameter and methods of analysis referred to in annexes 2 and 3. Procedures referred to in this paragraph can be handled via a single point. The examination of the application for determination of an investigative body must be completed within three months; section 42a paragraph 2 sentence 2 to 4 of the Administrative Procedure Act applies.
(8B) equivalent awards from another Member State of the European Union or another Contracting State to the agreement on the European economic area are of the same sentence 1 provisions referred to in paragraph 8. When the examination of the application for determination in accordance with paragraph 8 sentence 1 evidence from another Member State of the European Union or another Contracting State to the agreement on the European economic area are equally domestic evidence from them indicating that the applicant meets the relevant requirements of paragraph 8a set 1 or essentially comparable on the basis of their objective requirements of the exhibition State. The evidence shall be provided to the competent authority prior to commencement of activities in the original or copy. A certification of the copy, as well as a certified German translation may be required.
(9) in annex 1 column 3 for the separation, treatment and application of bio-waste 1 number laid down additional provisions must be observed.
(10) the paragraphs 1 to 9 are common hygienisierender treatment of bio-waste with in annex 2 mentioned materials on the entire material in line with to apply 1 number. Are already hygienisierend treated bio-waste, together 1 number with in annex 2 referred to materials of a subsequent biological stabilising treatment undergone, applies paragraph 4 sentence 1 number 3 with the proviso that the tests of the sanitized bio-waste making are only after treating biological stabilising of the duty-finished material. By way of derogation from sentence 2 tests the sanitized bio-waste can perform already after hygienisierenden treatment of the duty-finished material, if the subsequent biological stabilising treatment of the already hygienisierend treated bio-waste on a farm along with there fallen bio-degradable materials and the treated materials on self managed operation areas are built.

§ 3a requirements for biological stabilising treatment waste managers, producers and owners have, insofar as not by an exemption under § 10 paragraph 1 or paragraph 2 recycle bio-waste of boarding or the manufacture of mixtures of organic stabilizing treatment. Organic waste is to stabilize, that the common of good is not affected in particular by decomposition processes and odour burdens of applied organic waste or mixtures so far organic, taking into account the intended use.

§ 3 b treatment of bio-waste in farms with livestock production (1) on farms with livestock is allowed only after hygienisierenden treatment spending of organic waste of animal origin. Be kept farm animals at a factory in separate areas, sentence 1 only for these establishments shall apply.
(2) a treatment of bio-waste of animal origin in accordance with the § must perform only § 3 and 3a in farms with livestock, when the treatment plant is at a distance sufficient to protect against the transmission of infectious agents from the operating area, where the animals are kept. The operating range for the treatment of organic waste including acceptance, processing, storage and dispensing of the Bioabfallbehandler of animals is completely physically to separate feed and bedding, to ensure that the animals come with the organic waste of animal origin into contact either directly or indirectly. Sentences 1 and 2 shall apply accordingly to facilities for the treatment of bio-waste of animal origin on farms adjoining farms or establishments with livestock.

§ 4 the pollutants and other parameters (1) the Bioabfallbehandler requirements may only bio-waste and in annex 2 materials referred to use 1 number in unalloyed form due to their nature, quality or origin can be accepted after a treatment to comply with the requirements of paragraphs 3 and 4 and where there are no evidence of excessive levels of other than the pollutants covered by paragraph 3. In annex 1, point 2 called materials may be used too, if they meet the requirements of the fertilisers regulation on the material composition as fertilizers, soil enhancers or culture substrates and are no indication of excessive levels of other than pollutants covered by the fertilisers regulation. Concentrations of the pollutants referred to in sentences 1 and 2 are inflated when they intended use of organic waste or in annex 1, point the health of people or domestic and farm animals, health, growth and the quality of crops, the texture and fertility of the soil or the ecosystem could endanger 2 materials listed in unalloyed form.
(2) the Bioabfallbehandler the treated bio-waste may including in annex only in accordance with paragraphs 3 to 5 release 1 number of 2 called with treated materials or apply to self managed operation areas.
(3) the following heavy metals (milligrams per kilogram dry matter of to be material) must not be exceeded when applied in accordance with article 6, paragraph 1, sentence 1: lead cadmium 150 1.5 chrome 100 copper 100 nickel 50 mercury 1 zinc 400.
When applied in accordance with article 6, paragraph 1, sentence 2, following heavy metal content (milligrams per kilogram dry matter of to be material) must not be exceeded: lead 100 cadmium copper 1 chrome 70 70 nickel 35 mercury 0.7 zinc 300.
The values apply to copper and zinc pursuant to sentence 1 and 2 as having been observed, if the respective value in the moving average of four surveys conducted recently according to paragraph 5 is not exceeded and no analytical result exceed by more than 25 per cent. The competent authority for the application area can allow individual heavy metals exceeded in agreement with agricultural trade authority pursuant to sentence 1 with the exception of cadmium and mercury, if impairment of the well-being of the general public are not expected.
(4) the proportion of foreign substances, especially glass, plastic, metal, with a screen pass by more than 2 mm must not exceed referred to the dry mass of the material to be a maximum of 0.5 per cent. The proportion of stones with a screen pass by more than 10 mm must not exceed referred to the dry mass of the material to be accounted for 5 per cent.
(5) the Bioabfallbehandler has, not so far covered by an exemption under § 10 paragraph 1 or paragraph 2, per captive 2 000 tonnes of matter within the framework of the organic waste treatment including in annex 1 number of 2 called materials perform studies of treated bio-waste to the levels of heavy metals leave the pH lead, cadmium, chromium, copper, nickel, mercury and zinc and 2. on 1 , the salt content, the content of organic matter (loss on ignition), the dry residue and the share of foreign materials and stones.
The competent authority may in agreement with the competent authority of agricultural trade allow that studies of treated organic waste be performed starting at a quantity of more than 2 000 tonnes, the composition used according to type, composition and origin organic waste not or hardly changed. The competent authority can quality or origin of the used bio-waste order significantly changing composition style, that of treated bio-waste for quantities of less than 2 000 tonnes is being investigated. Notwithstanding sentences 1 to 3 are investigations of organic waste treated at a distance of no more than three months.
(6) by way of derogation from paragraph 5 sentence 1 may Bioabfallbehandler year more than 24 000 tonnes of matter organic waste including in annex 1 number of 2 called materials treat and are exempt according to § 11 paragraph 3 sentence 1 the presentation of research results or from proof obligations, once a month to carry out the investigations of treated bio-waste. Paragraph 5 sentence 2 to 4 shall apply mutatis mutandis.
(7) the Bioabfallbehandler sentence 1 has for that in paragraph 1 unmixed material referred to additional tests on the contents of paragraph 5 1 mentioned heavy metals set 1 number to have, if indications that sentence 1 not to adhere requirements mentioned in paragraph 3. The results of the competent authority according to the requirements set 1 not respected pursuant to paragraph 3, to submit without delay. The competent authority decides on the way forward. Until the decision of the competent authority, the treatment of the materials is prohibited. Paragraph 3 sentence 4 shall apply mutatis mutandis.
(8) the Bioabfallbehandler sentence 1 has in paragraph 1 unmixed material referred to or the treated bio-waste including in annex 1, point of 2 called with treated materials investigations on others as the pollutants covered by paragraph 3 to have, if in particular to the nature, quality or origin of the unblended single materials or organic waste treated evidence of excessive levels of these pollutants in the meaning of paragraph 1 sentence 3. Detected increased levels of these pollutants are the results of the competent authority to submit without delay. The competent authority decides on the way forward. Until the decision of the competent authority, the treatment, delivery and application of these materials is prohibited.
(9) the sampling, sample preparation, and investigations according to the paragraphs 5 to 8 are in accordance with the requirements of annex 3, and by independent, perform certain investigative bodies by the competent authority. The Bioabfallbehandler has to collect the results and to present the competent authority every six months. The results of the investigation are to store ten years. In the investigation of the organic waste treated an exceeding of the limit values for pollutants determined pursuant to paragraph 3, are immediately on the Bioabfallbehandler to transmit the findings of the investigating authority, this immediately forwards it to the competent authority.
(10) sentence 1 shall apply for the determination of an investigation authority pursuant to paragraph 9, section 3 paragraph 8a and 8B.

§ 5 may treated bio-waste requirements for mixtures (1) the manufacturer of the mixture, according to § 10, untreated, hygienisierend or stabilizing biologically treated bio-waste, as well as in annex 2 materials referred to use 1 number of which in unalloyed form due to their nature, quality or origin can be assumed, that they comply with the requirements of section 4, paragraph 3 and 4, and where no evidence of excessive levels other than those of article 4 paragraph 3 exist collected pollutants. In annex 1, point 2 called materials may be used too, if they meet the requirements of the fertilisers regulation on the material composition as fertilizers, soil enhancers or culture substrates and are no indication of excessive levels of other than pollutants covered by the fertilisers regulation. Article 4, paragraph 1, sentence 3 shall apply accordingly.
(2) the mixture manufacturers may only pursuant to sentences 2 to 4 give mixtures or apply to self managed operation areas. § 4 par. 3 to 6 and 9 shall apply accordingly. Section 4, paragraph 4, sentence 2 shall apply with the proviso that refers to the share of stones on the used bio-waste and the mixture for mixtures. § 4 paragraph 5 and 6 shall apply with the proviso that investigations of the mixture of each caught 2 000 tonnes produced mixture must be made.
(3) the manufacturer of the mixture has to allow additional research to the contents provided in § 4 paragraph 5, sentence 1 1 mentioned heavy metals carry number unalloyed materials listed, if evidence that the requirements are not complied according to § 4 paragraph 3 sentence 1 in paragraph 1. Section 4, paragraph 7, sentence 2 to 5 and article 9 shall apply mutatis mutandis.
(4) the manufacturer of the mixture has to leave if in particular to the nature, quality or origin for increased concentrations of these pollutants in the sense of § 4 paragraph 1 sentence 3 there are for the unalloyed materials referred to in paragraph 1 or the mixtures referred to in paragraph 2, recorded pollutants perform investigations on others than those of section 4, paragraph 3. § 4 paragraph 8 sentence 2 to 4 and article 9 shall apply mutatis mutandis.
(5) the additional terms 1 Nos. 1 and 2 in column 3 for the separation, treatment and use of the material, as well as the application of mixtures in annex be observed.

§ 6 restrictions and prohibitions of applying (1) without prejudice to fertilizer-legal regulations must be applied within on floors three years not more than 20 tons of dry organic waste or mixtures per hectare. The application of allowance in accordance with sentence 1 can be up to 30 tonnes per hectare three years if you measured heavy metal content does not exceed the limit values laid down in section 4, paragraph 3, sentence 2 according to § 4 paragraph 5 and 6 or § 5 paragraph 2. The authority responsible for the application area can allow more exceptions in individual cases in agreement with agricultural trade authority when the heavy metal limits referred to in section 4, paragraph 3, sentence 2 are significantly below and are not expected to impact the well-being of the general public.
(2) the application on soil of others listed in annex 1, point of 1 bio-waste referred to or of mixtures containing such organic wastes, requires the consent of the authority responsible for the organic waste treatment plant or mixture manufacturing plant in agreement with agricultural trade authority responsible for the application area. The competent authority shall carry out investigations on other pollutants in the sense of § 4 paragraph 8 sentence 1 having regard to the nature, to rearrange nature or origin of the bio-waste and providing the results prior to giving the consent agreement with agricultural trade authority against the debtor according to § 4 paragraph 2 and article 5, paragraph 2.
(2a) on tobacco acreage, tomato acreage in the field, as well as for vegetable and ornamental plant species in the protected cultivation only aerobic hygienisierend treated bio-waste and mixtures containing such bio-waste, must be applied.
(2B) bio-waste and mixtures may be provided only on or near the boarding area, insofar as this is necessary for the application.
(3) the application of bio-waste and forestry use soil mixtures may be only in justified exceptional cases with the consent of the competent authority in consultation with the competent forestry authorities.

Section 7 additional requirements when applied to grassland and field lining and field vegetable cultivation area (1) on grassland and more sleek field forage areas may only bio-waste and mixtures applied are listed in annex 1, point 1 column 3 and number 2 column 3 with a reference on this set are listed. In addition, bio-waste and mixtures on field forage areas may be placed on if these are applied and incorporated into the soil before the mounting of the box feed.
(2) on field vegetables surfaces, bio-waste and mixtures may be placed on, when they are applied and incorporated into the soil before the cultivation of field vegetables.
(3) organic waste and mixtures may contain no objects at application on grassland or field forage areas, which may cause injuries when shooting through domestic or farm animals.
(4) organic waste of animal origin or mixtures which contain such bio-waste, applied to grassland or field forage areas, a grazing may be only 21 days after the application of farm animals or a feed production. The competent authority for the application area may extend the period pursuant to sentence 1, if this is necessary to prevent a risk to human or animal health.

§ 8 May meeting of organic waste and sewage sludge application within the period according to § 6 paragraph 1 only the application of bio-waste and mixtures under this regulation or the application of sewage sludge to the sludge regulation in the same area.

Section 9 has (1) the farmers soil investigations or a responsible third party to specify the application surfaces to the competent authority within two weeks after the first occurring after October 1, 1998 application by bio-waste or mixtures. The competent authority shall the competent agricultural trade with these areas.
(2) for the first-time application of bio-waste or mixtures, a ground survey on heavy metals according to § 4 paragraph 5, sentence 1 is number 1 and perform on the Ph. The soil test results must be submitted no later than three months after the application of the competent authority. A valid soil testing is available for the application area after the sewage sludge Ordinance, these can be used accordingly. Sentence 1 shall not apply for the application of bio-waste and mixtures which are delivered by organic waste handlers and mixture manufacturers, which are exempt according to § 11 paragraph 3 sentence 1 the presentation of research results or from proof obligations. Are evidence that the land values of an application area the precautionary levels for soils according to annex 2 point 4.1 in connection with section 4.3 the Federal soil protection - and old loads regulation of 12 July 1999 (BGBl. I p. 1554), most recently by article 16 of the Act of July 31, 2009 (BGBl. I p. 2585) is changed, exceed, to prohibit the competent authority in consultation with the competent agricultural trade the renewed application of bio-waste or mixtures. Sampling, sample preparation and analysis is referred to in annex 1 of the sewage sludge Ordinance of 15 April 1992 (Federal Law Gazette I p. 912), most recently by article 9 of the Decree of 9 November 2010 (BGBl. I S. 1504) is has been modified, and through an independent, carried out by the competent authority certain investigative.
(2a) for an investigative record 6 provision referred to in paragraph 2 § 3 paragraph 8a and 8B according to.
(3) the competent authority for the application area can allow exemptions from the examination requirement referred to in paragraph 2 in agreement with the agricultural trade authority in a particular case when applied organic waste or mixtures within the meaning of § 6 paragraph 1 sentence 3.
(4) the competent authority may allow in agreement with the competent authority of agricultural trade in the framework of regional recovery for waive conditionally increased heavy metal contents of soils that biowaste or mixtures even on floors are applied, where the values referred to in paragraph 2 are exceeded, if no impairment of the well-being of the general public are to be expected. Sentence 1 does not apply to cadmium.

§ 9a additional requirements for the recycling of specific bio-waste (1) waste managers, producers and owners must submit 1, point 1(a) (b) referred to organic wastes only with the consent of the local authority in annex or apply to self managed operation areas. The bio-waste are type, composition, source and attack point the competent authority prior to initial submission or initial application on self managed operational areas, as well as significantly changing composition style, to specify quality or origin. The competent authority may require to assess the suitability of this bio-waste for recovery, investigation results about heavy metals and foreign content to be presented according to section 4, paragraph 3 and 4, documents other than the pollutants covered by article 4, paragraph 3 and additional ingredients, as well as more. Approval of the competent authority is not required for producers if at them not more than a total of two tons of in annex 1, point 1(a) (b) referred to organic wastes (small quantities) annually incurred.
(2) for the information referred to in paragraph 1 sentence 2 and 3 forms are cover disposal certificate (the), responsible explanation (VE) and Declaration analysis (DA) of annex 1 of the regulation by October 20, 2006 (Federal Law Gazette I p. 2298), by article 4 of the law of July 19, 2007 (BGBl. I S. 1462) is has been modified to use. The consent of the competent authority referred to in paragraph 1 sentence 1 is made using the standard form of authority confirmation (BB) of annex 1 of the regulation. The notes on the design of the forms from the footnote to the annex 1 of the regulation do not apply to the forms provided for pursuant to sentences 1 and 2. There is section 28 of the regulation apply mutatis mutandis for the required numbers.
(3) 1 debtor having rate referred to in paragraph 1 a copy of the full forms referred to in paragraph 2 sentence 1 and 2 once in the period of the approval of the authority at first supply of organic waste the Bioabfallbehandler or collector or in the case of the free organic waste treatment after handing section 10 the mixture manufacturers or operators of the application area.

§ 10 exemption from the requirements for the treatment and study of specific bio-waste (1) bio-waste may be unmixed delivered, making the mixture used or applied, insofar as these in annex 1, point 1 column 3 are listed and on one of the following numbers is referenced, 1 even without treatment, without hygienisierende treatment or biological stabilising treatment according to the articles 3 and 3a, and 2. in treated , hygienisierend treated, biological stabilising treated or untreated form without investigations after §§ 3 and 4 (2) the competent authority in agreement with the competent authority of agricultural trade in the framework of regional recovery in individual cases for more pure, homogeneous composite bio-waste exemptions referred to in paragraph 1 allow. The exemption of treatments according to the § § 3 and 3a can be granted if on the basis of the nature, quality or origin of the organic waste it can be assumed that the requirements laid down in sections 3 and 4 on the hygiene and be adhered to with regard to the pollutants and impurities and the well-being of the general public within the meaning of Article 3a (1) sentence 2 is not affected. Exemption from duties of examination of treated, hygienisierend treated, stabilizing biologically treated or untreated organic wastes may only be issued on the basis of the nature, quality or origin of the organic waste it can be assumed that the requirements set out in §§ 3 and 4 hygiene, as well as with regard to the pollutants and impurities are respected. The competent authority may before issuing the waivers of treatments and examinations pursuant to sections 3, 3a and 4 require that the hygienic safety investigations according to the tests of hygienisierten bio-waste according to article 3, paragraph 4, sentence 1 number 3, sentence 2 and paragraph 8 set 1 and the heavy metal contents and contents on other pollutants by investigations after § 4 paragraph 5, 8 and 9 set 1 detected. The exemption can be revoked at any time.
(3) as far as not included in an exemption under paragraph 1 or paragraph 2, are for sale, to apply use mixture manufacturing and application of untreated organic wastes according to the following regulations: 1 the tests of sanitized organic waste in accordance with article 3, paragraph 4, sentence 1 number 3, sentence 2, paragraph 7 and 8 sentences 1 and 2, 2. concerning investigations conducted pursuant to § 4 paragraph 5 , 6, 8 and 9, and 3. the documentation and evidence obligations in accordance with article 11, paragraph 1, sentence 1 and 2, paragraph 1 sentence 2 and 3 and paragraph 2 and 2a set 1 and 3.
That of set 1 resulting obligations of the organic waste handler are through the waste disposal authority, to meet the producer and the owner of the organic waste. When untreated, according to § 9a consent requiring bio-waste generation, the retention and template duties are b according to article 11, paragraph 1 to meet set 2 and 3 by the managers of the application area by using the copy of the full forms according to § 9a, paragraph 3.
(4) as far as not by an exemption pursuant to paragraph 1 or paragraph 2, recorded, are number 3 for the levy, the mixture production and application of exclusively biological stabilising treated bio-waste the provisions relating to the tests of sanitized organic waste in accordance with article 3, paragraph 4, sentence 1, sentence 1 and 2 according to apply to set 2, paragraph 7, and 8. The set 1 resulting obligations are to meet, performing the stabilizing biological treatment of organic waste by the Bioabfallbehandler.

§ 11 proof obligations
(1) the Bioabfallbehandler the materials used in the treatment has by type, source, quantity and attack instead of the original location of the attack until the last owner and list divided into batches of treated organic waste in accordance with sentences 2 and 3. Each batch of treated bio-waste is to be provided with a serial batch number, which must include at least the year and the month of treatment, as well as an ongoing treatment year numbering. It's a treatment plant with a continuous supply and removal of the treated material, the competent authority sets a certain amount of time in the Bioabfallbehandler to determine lots pursuant to sentence 2 has. The Bioabfallbehandler already hygienisierend treated or stabilizing biologically treated materials, used in a treatment he has to list them within the meaning of sentence 1 with the information referred to in paragraph 2 sentence 2 of the previous organic waste handler. Are the materials delivered to the Bioabfallbehandler by a single collector, this has split the collected materials pursuant to sentence 1 to list deliveries and to specify the Bioabfallbehandler according to type and quantity. In the case of the set eliminates the 4 and 5 for the Bioabfallbehandler which has documentation requirements of the attack site pursuant to sentence 1 (1a) of the mixture manufacturers divided into batches of produced mixture within the meaning of paragraph 1 sentence 1 to list the materials used in the mixing operations. Paragraph shall apply accordingly 1 sentence 2 and 4 to 6.
(1B) according to paragraph 1 and 1a debtor have the list be accompanied with the acquisition of the materials received delivery notes, commercial papers or other appropriate documentation as well as the copy of the full forms according to § 9a, paragraph 3. You have the list and to be included in documents from the time of creation of the lists be kept for ten years. These lists and documents of the competent authority shall be provided on request.
(2) Bioabfallbehandler and mixture manufacturers have to make a delivery note as defined in annex 4 the information for each emission of organic waste or mixtures applied on surfaces pursuant to sentence 2 and handed over to the managers of the application area or an intermediate cartridge. The delivery note must include the following information: 1. name and address of the issuing bio waste handler or mixture manufacturer (exhibitors), 2. name and address of the enterprise of the application area or of the intermediate cartridge, 3. batch number and amount, 4. levy as untreated, hygienisierend treated or stabilizing biologically treated bio-waste, treated bio-waste or as a mixture, as well as description of the organic waste or mixture to kind of unmixed materials , 5. insurance of the compliance with the requirements of a) to seuchen - and sanitary safety according to § 3 paragraph 2 and 3 and b) on the heavy metals according to § 4 paragraph 3, in conjunction with § 5 paragraph 2 sentence 2, measured 6 heavy metal content and appropriate pH, salinity, loss on ignition, dry residue and share of foreign materials and stones according to § 4 paragraph 5 and 6, also in connection with article 5, paragraph 2, sentence 2 and 4; a justification, when untreated, hygienisierend treated or organic stabilizing individual studies treated bio-waste in § 4 paragraph 5, sentence 1 number 2 above other parameters not feasible 7 study points and time of investigations in accordance with article 3, paragraph 4, sentence 1 are number 3, paragraph 7, 7a and 8, as well as § 4 paragraph 5, 6 and 9, also in connection with article 5, paragraph 2, sentence 2 and 4 , 8 maximum amount of application in accordance with article 6, paragraph 1, sentence 1, 2 or 3, 9 admissibility of application on grassland and more sleek field forage areas in accordance with § 7 paragraph 1, sentence 1, 10 date of the levy and the adoption and signature of the issuing bio waste handler or mixture producer (Exhibitor) and the enterprise of the application area or the intermediate customer.
The details are not required pursuant to sentence 2 number 5 to 7 as far as §§ 3, not to apply 3a and 4 are according to § 10. The intermediate customer has pursuant to sentence 2 number 2 and 10 in the original of the delivery note before the further delivery of materials to complement the information and handed over the delivery note to the managers of the application area or an additional intermediate cartridge.
(2a) the Bioabfallbehandler, the mixture manufacturers and the intermediate customer who gives the organic wastes and mixtures to the managers of the applied surface, have to send a copy of the fully completed delivery authority responsible for the application area, as well as the agricultural technical authority immediately after dispensing. The managers of the boarding area has immediately after applying the original of the delivery note uniquely identify the application area with the information to enter municipal area, hallway, hallway track number or alternative blow designation and the size in hectares, as well as the soil investigation according to article 9 paragraph 2 and to transmit a copy of the fully completed delivery authority responsible for the area of application, as well as the agricultural trade authority. The Bioabfallbehandler the mixture the intermediate customer, manufacturer of the managers of the applied surface have to keep the remaining with them copies of delivery from the moment of sending the copy to the competent authority for a decade.
(3) the competent authority may exempt from the template of test results according to § 3 paragraph 4 and 8, § 4 paragraph 5, and 9, also in connection with article 5, paragraph 2, sentence 2, as well as from the delivery procedure pursuant to paragraph 2 Bioabfallbehandler and manufacturer of the mixture;. an exemption may be granted from individual duties. An exemption may only be issued pursuant to sentence 1, if the Bioabfallbehandler or mixture manufacturers with regard to the treatment plant or mixture manufacturing plant is member of a carrier of a regular quality monitoring (Gütegemeinschaft) following its provisions a binding and continuous quality assurance is detected, and if the treatment plant or mixture production line 1 as a specialist disposal company is certified or 2nd as the EMAS site according to § 32 paragraph 1 sentence 1 of the Umweltauditgesetzes as amended by the notice of 4 September 2002 (Federal Law Gazette I p. 3490) , last by article 1 of the law of 6 December 2011 (Federal Law Gazette I p. 2509) is has been modified, is registered in the EMAS register. the registration is to inform the competent authority.
The competent authority can apply the determination of sentence 1 for Bioabfallbehandler and mixture manufacturers in agreement with the competent agricultural trade, which are members of a quality community, but number 1 or 2 do not meet the conditions of the set of 2. The competent authority can apply the provisions of the set 1 also for bio-waste in agreement with the competent authority of agricultural trade, which are exempt according to § 10 paragraph 1 or paragraph 2 of the treatment and examination duties.
(3a) for an exemption from the delivery in accordance with paragraph 3 sentence 1 Bioabfallbehandler and mixture manufacturers have to label the quality-assured bio-waste and mixtures, as well as according to § 10 paragraph 1 or paragraph 2 of the treatment and examination duties exempted organic waste at the release with the following information: 1. name and address of the issuing bio waste handler or mixture manufacturer and label the quality community, 2. batch number, 3. levy as untreated , hygienisierend treated or stabilizing biologically treated bio-waste, treated organic waste or as a mixture, 4. maximum amount of application in accordance with § 6, paragraph 1, sentence 1, 2 or 3, 5. admissibility of the application on grassland and more sleek field forage areas in accordance with article 7, paragraph 1, sentence 1.
Of the delivery procedure, liberated Bioabfallbehandler and mixture manufacturers, that the managers of the applied surfaces provide quality-assured bio-waste and mixtures to, have to provide documents to the authority responsible for the application area once a year for the past 12 months, which must contain the following information: 1. name and address of the issuing bio waste handler or mixture manufacturer, 2. AB-given name and address of the purchaser, 3. quantity in tonnes of dry matter (t TM) , 4. date of delivery.
Sentence 2 applies accordingly for intermediate customers, who submit quality-assured bio-waste and mixtures of organic waste handlers and mixture manufacturers, who are exempted from the delivery process to the managers of the application areas; in these cases is to number 1 in addition name and address of the organic waste handler or mixture manufacturer, who is a member of the Gütegemeinschaft specify including all intermediate buyers. The supporting documents shall be kept for ten years. The competent authority for the application area can the template of test results according to § 3 paragraph 4 and 8 as well as according to § 4 paragraph 5 and 9, also in connection with article 5, paragraph 2, sentence 2, and other appropriate evidence of the Bioabfallbehandler mixture manufacturers, intermediate buyers, or the institution of regular quality monitoring demand and withdraw the exemption at any time or the period and the period for the submission of documentation pursuant to sentence 2 , also in conjunction with sentence 3, shorten. The managers of the boarding area has immediately after the application of quality-assured bio-waste or mixtures of organic waste handlers or mixture manufacturers, who are exempted from the delivery procedure, applied materials, the quantity in tonnes of dry matter (t TM) and uniquely identify the application area with the information document municipal area, corridor, hallway track number or alternative beat label and the size in hectares and to submit the documentation to the competent authority upon request.
(4) on the recycling of bio-waste, subject to the provisions of this regulation, the provisions of the regulation with the exception of article 2, paragraph 1 find number 2, and of article 23 paragraph 2 of the Regulation No application.

Article 12 paragraph 2a sentence 2 do not apply article 9, paragraph 1 and 2 and article 11, if untreated or treated bio-waste or mixtures on surfaces of landowners applied exceptions for small areas, farm land used for agricultural or horticultural purposes not more than 1 hectare. § 11 paragraph 2a sentence 3 and paragraph 3a set 6 does not apply to the managers of these areas.

§ 12a created electronic data processing and delivery can in this regulation using electronic data processing prescribed documentation and evidence and with the consent of the competent authority, by electronic means or in electronic form submitted or transmitted.

Section 13 is number 8 of the circulatory economic law offences (1) any person in the meaning of § 69 paragraph 1, who intentionally or negligently not, not properly or in a timely manner brings them to 1 contrary to section 3, subsection 1 or § 3a paragraph 1 sentence 1 bio-waste of treatment, hygienisierende treatment does not or not correctly performing 2. contrary to article 3, paragraph 3, sentence 1, 3. contrary to § 3 spends b paragraph 1 sentence 1 bio-waste , 4. contrary to article 3 b, paragraph 2 sentence 2 not or not correctly separates a specified operating range, 5. contrary to § 4 paragraph 2 or article 5, paragraph 2, sentence 1 gives organic waste or a mixture or brings up 6 contrary to article 4, paragraph 7, sentence 1 or paragraph 8 set 1, also in conjunction with article 10, paragraph 3, sentence 1 an investigation not or not timely set 1 is number 2 and set 2, or section 5, paragraph 3, sentence 1 or paragraph 4 , 7. violates section 6, paragraph 1, sentence 1 or paragraph 2a or article 7, paragraph 1, sentence 1 bio-waste or a mixture brings up, 8 without permission according to § 6 paragraph 2 sentence 1 applies organic waste or a mixture, 9 contrary to section 8 brings up organic waste or a mixture and sewage sludge in the same area, contravenes an enforceable order according to article 9, paragraph 2, sentence 5 10 or 11 without permission according to § 9a paragraph 1 sentence 1 organic waste emits or brings up.
(2) any person within the meaning of § 69 paragraph 2 number 15 of circulatory economic law is, who intentionally or negligently the authority not, incorrectly, incompletely or not timely informed 1. contrary to article 3, paragraph 6, sentence 6, 2. contrary to a) section 3 (8) sentence 2, also in connection with article 10, paragraph 3, sentence 1 number 1 and set 2 or paragraph 4, b) article 3, paragraph 8, set 3 or c) article 4, paragraph 9, sentence 2 , also in connection with article 5, paragraph 2, sentence 2 or article 10, paragraph 3, sentence 1 number do not, not fully or not timely submit 2 and set 2, a result of the investigation, a recording or documentation, 3. contrary to article 3, paragraph 8, sentence 4 or § 4, paragraph 9, sentence 4 not, incorrectly, incompletely or not timely delivered a result or not, not properly , incompletely or not timely passes, 4. contrary to article 9, paragraph 1, sentence 1 an indication not, incorrectly, incompletely or not in time makes, 5. contrary to a) § 11 paragraph 1 sentence 1, also in conjunction with article 10, paragraph 3, sentence 1 number 3 and set 2, b) § 11 paragraph 1 sentence 4 or sentence 5, each also in combination with paragraph 1a sentence 2 , or c) § 11 paragraph 1a sentence 1 there called materials not, not properly or not lists, 6 contrary to section 11, subsection 1 sentence 2 in connection with article 10, paragraph 3, sentence 1 number 3 and set 2, a list, or a surface not or at least not for a decade kept, 7 an enforceable order according to § 11 para 1 sentence 3 , also in connection with article 10, paragraph 3, sentence 1 No. 3 and set 2, contravenes, 8 contrary to section 11, paragraph 2, sentence 1 or set 4 or paragraph 2a set 1 or set 3, also in connection with article 10, paragraph 3, sentence 1 number 3, sentence 2, a packing slip not, incorrectly, incompletely or not timely issued, a copy of the delivery note by a specified authority not that sent incorrectly, incompletely or not in time or a copy of the delivery note does not or at least not for a decade kept 9 contrary to section 11 paragraph 2a a copy of the delivery note of authority there referred not, incorrectly, incompletely or not timely sent to set 2 or 10 contrary to section 11 set 6 a paragraph 3a documentation shall not, incorrectly, incompletely or not in time.

§ 13a is on May 1, 2012 existing provisions for existing installations (1) when the plants where the requirements on the treatment isolated organic waste have been used according to § 10 paragraph 1 as amended by force until that date, and which will continue as the treatment plant to the sanitizing, perform a process review in accordance with article 3, paragraph 4, sentence 1 number 1 and set 2 18 months after May 1, 2012. Sentence 1 shall not apply as far as after September 30, 1993 for the plant or the induced process a hygiene check according to the specifications for the process audit or to comparable requirements was carried out or started and completes 12 months after May 1, 2012. In the case of the set 2, the Bioabfallbehandler has to present the findings on the hygiene audit according to the specifications for process testing or proof of the comparability of the hygiene test as well as the results of this hygiene inspection of the competent authority within three months after May 1, 2012 and to store ten years; If begun hygiene audit evidence and the investigation results within three months after completion of testing to submit and to store ten years. The competent authority may allow one of the carriers of the Gütegemeinschaft between 1 October 1998 and 1 may 2012 within the framework of the quality assurance process compliance audit 1 or 2 in agreement with agricultural trade authority when treatment plants pursuant to sentence 1 by organic waste handlers, who meet the requirements of § 11 paragraph 3 sentence 2 or 3, rather than the hygiene examination pursuant to sentence. With the conformity assessment must be demonstrated that the treatment plant or the used Hygienisierungsverfahren is a certified facility or a certified procedure according to the specifications for the process audit or to comparable requirements. The approval may only be issued if, after nature, quality or origin of the organic waste including in annex 1 number of 2 named materials is expected neither seuchen - and phytohygienischer issues.
(2) when the existing on May 1, 2012 pasteurization plants, which will continue as the treatment plant to the sanitizing, a technical inspection in accordance with article 3, paragraph 4, sentence 1 is number 1 and set 2 within 12 months after the May 1, 2012. Sentence 1 does not apply, as far as for the plant or the induced process a technical inspection according to the requirements of § 3 paragraph 4 sentence 1 number 1 and set 2 or is has been carried according to comparable requirements. In the case of the set 2, the Bioabfallbehandler has to submit the certificate of the technical inspection according to the requirements of this regulation or the proof of the comparability of the technical control of the competent authority within three months after May 1, 2012 and to store ten years.
(3) in the case of annexes existing on 1 may 2012, the Bioabfallbehandler has the requirements for process monitoring and the tests of sanitized organic waste in accordance with article 3, paragraph 4, sentence 1 number 2 and 3, sentence 2 after at least 12 months to comply.

§ 13b transitional provisions applicable and comparable hygiene checks, and applicable exception approvals
(1) direct process checks that number 1 in the version applicable up to this date have been conducted before May 1, 2012, according to article 3, paragraph 4, sentence 1, until the expiry of its validity, but no longer than up to the use of a new procedure or significant technical modification of the procedure or litigation, as a process audit in the sense of § 3 paragraph 4 sentence 1 number 1 for the sanitizing treatment plants shall continue to apply. Direct process examining similar hygiene tests, have been conducted prior to May 1, 2012, according to article 3, paragraph 5, sentence 3 and paragraph 8 sentence 3 in the version applicable up to that date for already existing plants and assigned by the competent authority, apply up to the expiry of its validity, at the latest however up to using a new process or significant technical modification of the procedure or litigation , as a process audit in the sense of § 3 paragraph 4 sentence 1 number 1 for the sanitizing treatment plants away.
(2) exception approvals, which have been issued prior to May 1, 2012, according to § 3 paragraph 3 sentence 2 in the version of the requirements contained in annex 2 to the direct process testing for treatment plants force until that date shall apply up to the expiry of its validity on, at the latest however up to using a new process or significant technical modification of the procedure or litigation. The competent authority later on at the latest twelve months should be exception approvals, remote process inspection and the are been issued before May 1, 2012, according to § 3 paragraph 3 sentence 2 in the force until this date version of the requirements contained in annex 2 inspection of treated bio-waste for treatment plants, After the expiry of the time limit, the demands on the process monitoring and the tests of sanitized organic waste in accordance with article 3, paragraph 4, sentence 1 are to keep number 2 and 3, sentence 2.

§ 14 entry into force annex 1 (to section 2 number 1, 4, 5, section 3, paragraph 3, 7, 7a, 9, 10, article 4, paragraph 1, 2, 5, 6, 8, article 5, paragraph 1, 5, article 6, paragraph 2, article 7 paragraph 1, Article 9a, paragraph 1, article 10 paragraph 1, section 13a, paragraph 1) list of organic waste suitable for recycling on land as well as other appropriate waste, biodegradable materials, and mineral substances (site : BGBl. I 2013, 671-688) 1 organic waste in accordance with § 2 number 1 a) bio-waste, requiring section 9a to the recycling waste label any consent in accordance with the system of the AVV1 (in brackets: waste key) appropriate Abfälle2 of the waste labels supplementary provisions referred to in column 1 (in brackets: waste origin according to the header of the plant of the AVV1) sludges from washing and cleaning processes (02 01 01)-fish pond mud, fish pond sediments and filter sludges from the fish production (waste from agriculture , Horticulture, aquaculture, forestry, hunting and fisheries) the materials are suitable waste referred to in column 2, if these are not mixed with effluents or sludges outside the specific production at the point of attack.
The materials are exempt when applied in the context of regional recovery according to § 10 para 1 Nos. 1 and 2 of the treatment and examination duties.
Plant tissue waste (02 01 03) - hemp and flax shives - coir - vegetable waste from the gardening - vegetable waste from the water entertainment - vegetable waste from agriculture - vegetable waste from the fish farming and fishing - herbal filter materials in the biological purification of waste Reed - husk, husk - and grain dust (wastes from agriculture, horticulture, aquaculture, forestry, hunting and fisheries) herbal filter materials from the biological purification of suitable waste referred to in column 2 are , when these fall within the framework of the production and processing of food and feed, animal by-products, and stables.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas; the biological purification of waste vegetable filter materials are excluded.
Waste plastics (except packaging) (02 01 04)-biologically degradable materials (plastics) from predominantly renewable raw materials (wastes from agriculture, horticulture, aquaculture, forestry, hunting and fisheries) appropriate waste referred to in column 2 are such as drop cloths.
The materials are suitable waste referred to in column 2, when according to DIN EN 13432 (Edition 2000-12) and DIN EN 13432 correction 2 (Edition 2007-10) or DIN EN 14995 (Edition 2007-03) are certified.
 
 
The materials are free according to § 10 para 1 Nos. 1 and 2 of the treatment and examination duties, if they are incorporated in the attack position in the ground.
Animal excreta, manure/slurry and manure (including spoiled straw), effluent, collected separately and treated off-site (02 01 06) - old straw - animal excretions, also with bedding (wastes from agriculture, horticulture, aquaculture, forestry, hunting and fishing) the provisions of this regulation are for animal excretions, also with litter, only applicable as far as them not subject as animal by-products (liquid manure from livestock) of Regulation (EC) No. 1069 / 20093.
Infectious materials are not suitable waste referred to in column 2.
Old straw and animal excrement, also with bedding, recorded separately or mixed together, are exempt when applied in the context of regional recovery according to § 10 paragraph 1 Nos. 1 and 2 of the treatment and examination duties.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Wastes from forestry (02 01 07) - also unmixed processed natural vegetable waste from forestry, vegetable waste from forestry (wastes from agriculture, horticulture, aquaculture, forestry, hunting and fishing), are exempted pursuant to section 10 paragraph 1 number 1 of the treatment obligations.
In the context of a composting the materials to crush or the compost so extents are that there are no lumpy materials over 40 mm (sieve mesh size) in the compost.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Wastes not otherwise specified.
(02 02 99)-herbal filter materials in the biological purification of waste (waste from the preparation and processing of meat, fish and other foods of animal origin) herbal filter materials in the biological purification of waste are suitable waste referred to in column 2, if applicable in the context of the manufacture and processing of food and feed products and animal by-products.
Waste from the extraction with solvents (02 03 03) - vegetable residues resulting from the extraction with alcohol (wastes from the preparation and processing of fruit, vegetables, cereals, edible oils, cocoa, coffee, tea and tobacco, from canning, the production of yeast and yeast extract as well as the preparation and fermentation of molasses) may the materials, as part of a mixture, according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas be applied.
Materials unsuitable for consumption or processing (02 03 04) - old flour - fermentation residues of the enzyme and vitamin production - grain waste - yeast and yeast-like residue - coir - molasses residues - oilseed residues - vegetable amino acids - vegetable oils and fats - rape extraction meal, rapeseed cake - Castor - residues from potato, corn or rice starch production - residues from the preparation and processing of coffee, tea and cocoa - residues from the preparation and processing of fruit, vegetable and cereal - residues from canning - residues of spices and vegetable seasonings - potato Peel holdings - husk residues , Husks - and grain dust - layered-grus, tobacco dust and RIP - layered fine food - food - spent filtration and absorbent mass (bleaching Earth, Cellite de-oiled, kieselguhr, perlite) - Vinasse and Vinasserückstände - cigarette off-specification batches (wastes from the preparation and processing of fruit, vegetables, cereals, edible oils, cocoa, coffee, tea and tobacco, from canning, the production of yeast and yeast extract as well as the preparation and fermentation of molasses) are the provisions of this regulation for higher-level food , Residues from canning and layered animal origin food only applicable, as far as this or major material components rather than animal by-products of Regulation (EC) No. 1069 / 20093 are subject to.
Digestion residues from the production of the vitamin are suitable waste referred to in column 2, if applicable within the framework of the production of vitamin B2.
The recovery of edible vegetable oils and fats is allowed only with anaerobic treatment.
Castor is suitable waste referred to in column 2, if this harmless amounts of ricin (no acute oral toxicity by up to 2 000 mg Castor/kg body weight in rats) has. Castor is to treat, that a recording is stopped by animals with means (denaturing) He must not be mixed with substances represent an incentive for the recording by animals.
 
 
Separately collected diatomaceous earth is free when applied in the context of regional recovery according to § 10 para 1 Nos. 1 and 2 of the treatment and examination duties. Mixtures containing diatomaceous earth and diatomaceous earth must not be placed in the dry state and are in the application, to incorporate into the ground immediately.
Cigarette off-specification batches are suitable waste referred to in column 2 if they contain no filter and no wrapper.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas; Digestion residues of the enzyme and vitamin production, plant amino acids, Castor, residues from the preparation and processing of coffee, tea and cocoa, tobacco dust,-grus and - RIP, diatomaceous earth and cigarette off-specification batches are excluded.
Wastes not otherwise specified.
(02 03 99)-herbal filter material from the biological Abluft-cleaning (wastes from the preparation and processing of fruit, vegetables, cereals, edible oils, cocoa, coffee, tea and tobacco, from canning, the production of yeast and yeast extract as well as the preparation and fermentation of molasses) herbal filter materials in the biological purification of waste are suitable waste referred to in column 2, if applicable in the context of the manufacture and processing of food and feed products and animal by-products.
Wastes not otherwise specified.
(02 04 99) - residues of molasses - plant filter material from the biological purification - crimping, wet and dried pulp - beet small parts and turnip tops - Vinasse and Vinasserückstände - sugar beet pulp and-presskuchen (waste from sugar production) herbal filter materials in the biological purification of waste are suitable waste referred to in column 2, if applicable in the context of the manufacture and processing of food and feed.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas; the biological purification of waste vegetable filter materials are excluded.
Wastes not otherwise specified.
(02 05 99)-herbal filter materials in the biological purification of waste (waste from the processing of milk) herbal filter materials in the biological purification of waste are suitable waste referred to in column 2, if applicable in the context of the manufacture and processing of food and feed products and animal by-products.
Materials unsuitable for consumption or processing (02 06 01) - old flour - digestion residues from the production of the enzyme - layered yeast and yeast-like residue - dough waste - food - layered food (waste from the production of bakery products and confectionery) the provisions of this regulation are layered food and dough waste of animal origin only applicable for, as far as this or major material components rather than animal by-products of Regulation (EC) No. 1069 / 20093 are subject to.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Wastes not otherwise specified.
(02 06 99)-herbal filter materials in the biological purification of waste (waste from the production of bakery products and confectionery) herbal filter materials in the biological purification of waste are suitable waste referred to in column 2, if applicable in the context of the manufacture and processing of food and feed products and animal by-products.
Waste from the distillation of alcohol (02 07 02) - fruit, cereal and potato dregs (wastes from the production of alcoholic and non-alcoholic beverages [without coffee, tea and cocoa]) may the materials, as part of a mixture, according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas be applied.
Materials unsuitable for consumption or processing (02 07 04) - Draff - yeast and yeast-like residue - hop Marc - malt grains, malt sprouts, malt dust - molasses residue - pulp - layered food - layered drinks - spent filtration and absorbent mass (Cellite, kieselguhr, perlite) - separated Vinasse and Vinasserückstände (waste from the production of alcoholic and non-alcoholic beverages [without coffee, tea and cocoa]) diatomaceous earth covered is optional when applied in the context of regional recovery according to § 10 para 1 Nos. 1 and 2 of the treatment and examination duties. Mixtures containing diatomaceous earth and diatomaceous earth must not be placed in the dry state and are in the application, to incorporate into the ground immediately.
 
 
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas; diatomaceous earth is excluded.
Wastes not otherwise specified.
(02 07 99)-herbal filter materials in the biological purification of waste (waste from the production of alcoholic and non-alcoholic beverages [without coffee, tea and cocoa]) herbal filter materials in the biological purification of waste are suitable waste referred to in column 2, if applicable in the context of the manufacture and processing of food and feed.
Waste bark and Cork (03 01 01) - bark (wastes from wood processing and the production of panels and furniture) separated gripped by natural bark, that also unmixed processed, are exempt from the treatment obligations according to § 10 para 1 No. 1.
In the context of a composting the materials to crush or the compost so extents are that there are no lumpy materials over 40 mm (sieve mesh size) in the compost.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Sawdust, shavings, sections, wood, particle board and veneer other than those mentioned in 03 01 04 (03 01 05) - wood shavings - saw dust and sawdust (wastes from wood processing and the production of panels and furniture) sawdust, wood shavings and sawdust are suitable waste referred to in column 2, if they manufactured from untreated wood or attacked are.
In the context of a composting, sawdust to crush or the compost so extents are that there are no lumpy materials over 40 mm (sieve mesh size) in the compost.
Saw dust and sawdust from Virgin wood from the area of wood processing, are allowed to, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Bark and wood waste (03 03 01) - bark (wastes from the production and processing of paper, pulp and paperboard) separately collected, natural bark and unmixed processed further bark are exempt from the treatment obligations according to § 10 para 1 No. 1.
In the context of a composting the materials to crush or the compost so extents are that there are no lumpy materials over 40 mm (sieve mesh size) in the compost.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Geäschertes glue leather (04 01 02)-Geäschertes glue leather (wastes from the leather and fur industry) Geäschertes glue leather is suitable waste referred to in column 2, if this comes from the processing of hides the category 3 in accordance with Regulation (EC) No. 1069 / 20093.
Geäschertes glue leather pursuant to annex XIII chapter V point C paragraph 2 of Regulation (EU) No. 142 / 20114 applies in accordance with § 3 paragraph 3 set 5 in conjunction with article 2 paragraph 2 letter d as otherwise hygienisierend treated and is exempted pursuant to section 10 paragraph 1 number 2 of the investigation duties according to § 3.
The exploitation of the materials is allowed only with anaerobic treatment.
Wastes from untreated textile fibres (04 02 21) - fibre waste - wool waste - Cellulose fiber waste (waste from the textile industry) the provisions of this regulation are only applicable for wool waste of animal origin, as far as these not subject as animal by-products (raw materials) of Regulation (EC) No. 1069 / 20093.
Wastes not otherwise specified.
(07 01 99) - fat, grease and oil from the production of bio-diesel - dregs from the production of technical alcohols (wastes from the manufacture, formulation, supply and use of organic basic chemicals) are the provisions of this regulation for fat, grease and oil of animal origin from the production of bio-diesel only applicable if not No. 1069 / 20093 subject to as animal by-products of Regulation (EC).
 
 
The utilization of fat, grease and oil from the production of bio-diesel is allowed only with anaerobic treatment.
Solid wastes other than those mentioned in 07 05 13 (07 05 14) - medicines and medicinal plants and herbs - mycelium - mushroom substrate residues - vegetable amino acids - vegetable protein - vegetable protein waste - residues of medicines and medicinal plants and herbs - residue of medicines and medicinal plants (wastes from the manufacture, formulation, supply and use of pharmaceuticals) mycelium from drug manufacturing may be recovered only after individual assessment and appropriate waste referred to in column 2 , if no effective drug residues are included.
Mushroom substrate residues, where the fungal cultures has been proven by damping killed 2(d) as otherwise be dealt with article 2 number hygienisierend according to § 3 paragraph 3 set 5 in connection and are exempted pursuant to section 10 paragraph 1 paragraph 2 of the investigation duties according to § 3.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas; mycelium, plant amino acids, vegetable protein and vegetable protein waste are excluded.
Wastes whose collection and disposal is no special requirements are infection-preventive (e.g. B. wound and plaster casts, linen, disposable clothing, diapers) (18 01 04)-Moor mud and clay (wastes from the obstetrics, diagnosis, treatment or prevention of disease in humans) Moor mud and clay are suitable waste referred to in column 2, if they do not contain drug residues.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Grease and oil mixture from oil/water separation containing only edible oil and fats (19 08 09)-contents of grease traps (wastes from waste water treatment plants not otherwise specified.)
The exploitation of the materials is allowed only with anaerobic treatment.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Paper and cardboard (20 01 01)-paper (separately collected fractions of waste [except 15 01]) waste paper may be admitted only in small amounts (up to 0.5%) for composting.
The addition of waste paper is in small amounts together with separately collected bio-waste (waste entry 20 03 01) allowed if this is appropriate for hygienic or practical reasons (for example, when very wet organic waste).
The recovery of glossy paper and paper from old wallpaper is not permitted.
Biodegradable kitchen and canteen waste (20 01 08) - biological biodegradable kitchen and canteen waste - content of fat separators (separately collected fractions of waste [except 15 01]) the provisions of this regulation are only applicable for biodegradable kitchen and canteen waste of animal origin, as far as them not subject as animal by-products of Regulation (EC) No. 1069 / 20093.
The exploitation of the content of grease traps is allowed only with anaerobic treatment.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Edible oils and fats (20 01 25)-edible oils and fats (separately collected fractions of waste [except 15 01]) the provisions of this regulation are only applicable for edible oils and fats of animal origin, as far as these not subject as animal by-products (kitchen and canteen waste or higher-level food) Regulation (EC) No. 1069 / 20093.
The exploitation of the materials is allowed only with anaerobic treatment.
Edible oils and fats of vegetable origin, are allowed to, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Plastics (20 01 39) - biological degradable materials (plastics) from predominantly renewable raw materials (separately collected fractions of waste [except 15 01]) materials are suitable waste referred to in column 2, if according to DIN EN 13432 (Edition 2000-12) and DIN EN 13432 correction 2 (Edition 2007-10) or DIN EN 14995 (Edition 2007-03) are certified; Waste bags, which are the collection of biodegradable waste as determined for example by the kitchen and canteen waste.
Biodegradable waste (20 02 01) - organic biodegradable waste by sports facilities, places, ruins, and children's playgrounds (as far as not garden and Park waste) 5 - biological degradable cemetery waste - biological biodegradable garden and Park waste - wood clearing residues (as far as not garden and Park waste) 5 - Landschaftspflegeabfälle5 - vegetable waste from waters entertainment (as far as not garden and Park waste) 5 - herbal components of the Treibsels (including coastal and riparian areas) 5 (garden and Park waste [including cemetery waste]) within the framework of a composting are woody materials or the compost so extents to crush , that no piece materials over 40 mm (sieve mesh size) contained in the compost.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas; vegetable materials of road loading sliding surfaces are excluded (roads, paths, triggered, airports) and of industrial sites.
Mixed Siedlungsabfälle6 (20 03 01) - separately collected bio-waste of household and the small industry (especially bio bin) collected Bioabfälle6 (other municipal waste) appropriate waste referred to in column 2 are separated.
Market waste (20 03 02) - vegetable market waste (other waste) may the materials, as part of a mixture, according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas be applied.
b) bio-waste, requiring section 9a to the recycling waste designation a consent in accordance with the system of the AVV1 (in brackets: waste code) appropriate Abfälle2 of the waste labels supplementary provisions referred to in column 1 (in brackets: waste origin according to the header of the plant of the AVV1) sludges from washing and cleaning processes (02 01 01)-other mud-shaped food waste (wastes from agriculture, horticulture, aquaculture, forestry, hunting and fisheries) the provisions of this regulation are only applicable for other mud-shaped food waste of animal origin , as far as not as animal by-products of Regulation (EC) No. 1069 / 20093 are subject to.
 
 
The materials are suitable waste referred to in column 2, if these are not mixed with effluents or sludges outside the specific production at the point of attack.
Other mud-shaped food waste may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Wastes not otherwise specified.
(02 01 99)-mushroom substrate residues (wastes from agriculture, horticulture, aquaculture, forestry, hunting and fisheries) appropriate waste referred to in column 2 are worn substrates from the edible mushroom production.
Mushroom substrate residues, where the fungal cultures has been proven by damping killed 2(d) as otherwise be dealt with article 2 number hygienisierend according to § 3 paragraph 3 set 5 in connection and are exempted pursuant to section 10 paragraph 1 paragraph 2 of the investigation duties according to § 3.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Sludges from on-site effluent treatment (02 02 04) - contents of grease traps and residues - production specific sludges from on-site effluent treatment - sludge from the manufacture of gelatine (wastes from the preparation and processing of meat, fish and other foods of animal origin) the materials are suitable waste referred to in column 2, if these are not mixed with effluents or sludges outside the specific production at the point of attack.
The contents of fat separators and the residues is permissible only with anaerobic treatment.
Separately collected gelatin lime sludges that are demonstrably sanitize with caustic soda and lime, apply in accordance with § 3 paragraph 3 set 5 in conjunction with article 2 number 2 letter d as otherwise hygienisierend handled and are exempted pursuant to section 10 paragraph 1 number 2 of the investigation duties according to § 3.
 
 
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Sludges from washing, cleaning, peeling, centrifuging - and separation (02 03 01)-other mud-shaped food waste (waste from the preparation and processing of fruit, vegetables, cereals, edible oils, cocoa, coffee, tea and tobacco, from canning, the production of yeast and yeast extract as well as the preparation and fermentation of molasses)
The provisions of this regulation are only applicable for other mud-shaped food waste of animal origin, as far as these not subject as animal by-products of Regulation (EC) No. 1069 / 20093.
The materials are suitable waste referred to in column 2, if these are not mixed with effluents or sludges outside the specific production at the point of attack.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Materials unsuitable for consumption or processing (02 03 04) - sludge from the production of vegetable fats - sludge from the production of vegetable oils - strength mud - tobacco sludge (wastes from the preparation and processing of fruit, vegetables, cereals, edible oils, cocoa, coffee, tea and tobacco, from canning, the production of yeast and yeast extract as well as the preparation and fermentation of molasses) materials are suitable waste referred to in column 2 , when these are not mixed with effluents or sludges outside the specific production at the point of attack.
The is permissible only with anaerobic treatment of sludge from the Edible fat and oil production.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas; Tobacco slurry is excluded.
Sludges from on-site effluent treatment (02 03 05) - contents of grease traps and residues - production specific mud from operating own effluent treatment (wastes from the preparation and processing of fruit, vegetables, cereals, edible oils, cocoa, coffee, tea and tobacco, from canning, the production of yeast and yeast extract as well as the preparation and fermentation of molasses) the materials are suitable waste referred to in column 2, if these are not mixed with effluents or sludges outside the specific production at the point of attack.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Sludges from on-site effluent treatment (02 04 03)-production specific sludges from on-site effluent treatment (waste from sugar production) the materials are suitable waste referred to in column 2, if these are not mixed with effluents or sludges outside the specific production at the point of attack.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Sludges from on-site effluent treatment (02 05 02) - contents of grease traps and residues - production specific sludges from on-site effluent treatment (waste from the processing of milk) the materials are suitable waste referred to in column 2, if these are not mixed with effluents or sludges outside the specific production at the point of attack.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Sludges from on-site effluent treatment (02 06 03) - contents of grease traps and residues - production specific sludges from on-site effluent treatment (waste from the production of bakery products and confectionery) the materials are suitable waste referred to in column 2, if these are not mixed with effluents or sludges outside the specific production at the point of attack.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Waste from the distillation of alcohol (02 07 02) - sludge from distillery (wastes from the production of alcoholic and non-alcoholic beverages [without coffee, tea and cocoa]) may the materials, as part of a mixture, according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas be applied.
Materials unsuitable for consumption or processing (02 07 04) - Lees and sludge from breweries - Lees and mud from fruit juice production - may the materials Lees and mud from wine production (waste from the production of alcoholic and non-alcoholic beverages [without coffee, tea and cocoa]), as part of a mixture, according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas be applied.
Sludges from on-site effluent treatment (02 07 05)-production specific sludges from on-site effluent treatment (waste from the production of alcoholic and non-alcoholic beverages [without coffee, tea and cocoa]) the materials are suitable waste referred to in column 2, if these are not mixed with effluents or sludges outside the specific production at the point of attack.
The materials may be applied, as part of a mixture according to § 7 paragraph 1 sentence 1 on grassland and more sleek field forage areas.
Wastes not otherwise specified.
(07 01 99)-glycerol from the production of bio diesel (wastes from the manufacture, formulation, supply and use of organic basic chemicals) glycerol from the production of biodiesel is suitable waste referred to in column 2, if this a minimum salary of 70% is raw glycerine and a residual methanol content of not more than 3%.
The exploitation of the materials is allowed only with anaerobic treatment.
2. other waste and bio-degradable materials and minerals, for a common treatment with bio-waste (§ 2 number 4) and for the manufacture of mixtures (§ 2 number 5) are suitable if waste, waste designation according to the system of the AVV1 (in brackets: waste code) allowed other Abfälle2 of the labels of waste referred to in column 1, biodegradable materials and minerals supplementary provisions (necessary in parentheses : Waste origin according to group heading the plant of AVV1) waste gravel and crushed rocks other than those mentioned in 01 04 07 (01 04 08) - dolomite waste - limestone waste (waste from physical and chemical processing of non-metalliferous minerals) waste from sand and clay (01 04 09) - Sandy - dusty and powdery wastes other than those mentioned ton (wastes from physical and chemical processing of non-metalliferous minerals) , in 01 04 07 (01 04 10)-rock flour (wastes from physical and chemical processing of non-metalliferous minerals) not just to specification calcium carbonate mud (02 04 02) - Carbonatationsschlamm (waste from sugar production) may also bio-waste materials according to § 7 paragraph 1 sentence 1 and admitted mixtures which, applied on grassland and more sleek field forage areas.
Lime mud waste (03 03 09)-fiber lime (wastes from the production and processing of paper, pulp and paperboard) fiber lime is allowed other waste referred to in column 2, if this comes from the processing of virgin fiber of the manufacture of white paper and any precipitation agents (except lime) are admitted.
Rust, bottom ash, slag and boiler dust with the exception of boiler dust, in 10 01 04 falls (10 01 01) - ash from the burning of brown coal - ash from the combustion of natural botanicals - ash from the combustion of materials of animal origin - ash from the incineration of paper (wastes from power stations and other combustion plants [except 19]) ash from the incineration of paper is allowed other waste in accordance with column 2 , if it is within the framework of the energetic use of paper residues from the manufacture of paper.
The materials are permissible other wastes in accordance with column 2, if applicable as a combustion chamber ash or ashes from the combustion of vortex layer. Materials, as ash from the last filtering unit in the flue gas path or condensate filter sludge incurred, are not permitted other waste in accordance with column 2 used chemicals other than those mentioned in 16 05 06, 16 05 07 or 16 05 08 (16 05 09)-ABC fire (gas in pressure vessels and used chemicals) bottom ash and slag other than those mentioned in 19 01 11 (19 01 12) - ash from the combustion of natural botanicals - ash from the combustion from materials of animal origin - ash from the incineration of sewage sludge - ash from the incineration of paper (wastes from incineration or pyrolysis of waste) ash from the incineration of sewage sludge is allowed other waste referred to in column 2, if the sewage sludge from the treatment of urban waste water in accordance with the sewage sludge Ordinance.
 
 
Ash is permissible other waste referred to in column 2 from the incineration of paper if they incurred in connection with the energetic use of paper residues from the manufacture of paper.
The materials are permissible other wastes in accordance with column 2, if applicable as a combustion chamber ash or ashes from the combustion of vortex layer. Materials incurred as ashes from the last filtering unit in the flue gas path or condensate filter sludge, are not permitted other waste in accordance with column 2. waste a. n. g.
(19 08 99)-mud from the phosphate precipitation with lime (wastes from waste water treatment plants not otherwise specified.)
The phosphate precipitation with lime sludge is allowed other waste referred to in column 2, if this comes from urban waste water treatment plants.
The materials may be specified to bio-waste and mixtures according to § 7 paragraph 1 sentence 1, are applied on grassland and more sleek field forage areas.
Sludges from the decarbonation (19 09 03) - sludge from water softening (wastes from the preparation of water intended for human consumption or industrial hot water) materials incurred as the sludge from the iron and the manganese removal, are not permitted other waste referred to in column 2.
The materials may be specified to bio-waste and mixtures according to § 7 paragraph 1 sentence 1, are applied on grassland and more sleek field forage areas.
(If materials in individual cases are waste according to recycling and waste law, mapping to a waste label)-materials in accordance with Düngemittelverordnung7: • fertilizer according to § 3 DüMV and manure, soil enhancers and growing mediums in accordance with § 4 DüMV • substances listed in the tables 6, 7 (with the exception of sewage sludge by number 7.4.3) and 8 (with the exception of pollutants referred to under 8.3.11 column 3 last sentence) Appendix 2 DüMV materials according to Düngemittelverordnung7 are allowed other waste , bio-degradable materials and mineral substances listed in column 2, as far as these are not called as bio-waste as permissible other wastes in other table rows of this number or number 1.
If fertilizers and raw materials of animal origin as the animal by-products of Regulation (EC) No. 1069 / 20093 are subject to, are also to apply their provisions.
 
 
The materials may be specified to bio-waste and mixtures according to § 7 paragraph 1 sentence 1, are applied on grassland and more sleek field forage areas, insofar as the application of the materials on these surfaces is allowed according to the Düngemittelverordnung7 or the Düngeverordnung7.
-Animal by-products under Regulation (EC) No. 1069 / 20093: • category 3 in accordance with article 10 Regulation (EC) no 1069/2009, • category 2 in accordance with article 9 letter (a) Regulation (EC) No. stomach 1069/2009 (manure, non-mineralised guano, gastric and intestinal contents and rumen content) and intestinal contents and rumen content are permissible biodegradable materials in accordance with column 2, if they come from animals which are slaughtered for human consumption.
The materials may be specified to bio-waste and mixtures according to § 7 paragraph 1 sentence 1, are applied on grassland and more sleek field forage areas, insofar as the application of the materials on these areas is allowed under Regulation (EC) No. 1069 / 20093.
-Renewable renewable raw materials are permissible biodegradable materials in accordance with column 2, as far as they are not known as bio-waste in number 1.
In the context of a composting, woody materials to crush or the compost so extents are that there are no lumpy materials over 40 mm (sieve mesh size) in the compost.
The materials may be specified to bio-waste and mixtures according to § 7 paragraph 1 sentence 1, are applied on grassland and more sleek field forage areas.
-Ground materials ground materials are permissible biodegradable materials and mineral substances listed in column 2, if this the precautionary levels for soils pursuant to annex 2 paragraph 4 the Federal soil protection and old loads regulation do not exceed.
The materials may be specified to bio-waste and mixtures according to § 7 paragraph 1, are applied to grassland.
3. notices of expert bodies were DIN standards referenced in this Appendix at the Beuth-Verlag GmbH, Berlin, published and are laid down in terms of archive secured at the German patent and trade mark Office in Munich.
1 list of waste regulation (GCU) of 10 December 2001 (BGBl. I S. 3379), most recently by article 5 paragraph 22 of the Act of February 24, 2012 (BGBl. I S. 212) has been changed.
2 waste according to the waste type catalog of the country working group waste, 16 working group waste: LAGA briefing paper waste - 1991, releases the land working group waste (LAGA) - Erich Schmidt Verlag, Berlin.
3 Regulation (EC) no 1069/2009 of the European Parliament and of the Council of 21 October 2009 laying hygiene requirements for animal by-products not intended for human consumption and repealing Regulation (EC) No. 1774/2002 (animal by-products regulation) (OJ L 300 from November 14, 2009, p. 1), the last by Directive 2010/63/EC (OJ L 276 of 10, p. 33) is has been modified in the currently valid version.
4 Regulation (EU) No 142 / 2011 of the Commission by February 25, 2011, for the implementation of Regulation (EC) no 1069/2009 of the European Parliament and of the Council with health rules on animal by-products not intended for human consumption, as well as to the implementation of Directive 97/78/EC of as regards certain in accordance with that directive by veterinary checks on the border of free samples and goods (OJ OJ L 54 of the 26.2.2011, p. 1).
5 the waste materials associate with this waste label, because the AVV Bosk grubbing-up residue and vegetable waste from the water entertainment as well as landscape waste and herbal components of the Treibsels contains no special waste label for falling outside of gardens and parks of biodegradable waste by sports facilities, places, ruins, and children's playgrounds.
6 the waste materials associate with this waste label, because the AVV contains no special waste label for separately collected bio-waste, in particular in bio tons.
7 regulation of fertilizers and minimum in the currently valid version.

Annex 2 (§ 2 number 2, § 3, paragraph 2 to 7) requirements on the sanitiser treatment of bio-waste to ensure the seuchen-and sanitary safety table of contents 1 General comments 2 sanitiser treatment 2.1 treatment procedures to the sanitizing (§ 2 number 2) 2.2 requirements the sanitiser handling 2.2.1 pasteurization 2.2.1.1 requirements of 2.2.1.2 process management process testing (to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) 2.2.1.3 process monitoring (to article 3, paragraph 4, sentence 1 number 2 i. V.) m. paragraph 6) 2.2.1.4 tests of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 number 3 i. V. m. paragraph 7 and 7a) 2.2.2 aerobic hygienisierende treatment (thermophilic composting) 2.2.2.1 requirements on the litigation 2.2.2.2 process testing (to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) 2.2.2.3 process monitoring (to article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6) 2.2.2.4 tests of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 number 3 i. V. m.) Paragraph 7 and 7a) 2.2.3 hygienisation anaerobic treatment (thermophilous fermentation) 2.2.3.1 2.2.3.2 process management requirements determination of the minimum dwell time 2.2.3.3 process testing (to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) 2.2.3.4 process control (to article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6) 2.2.3.5 tests of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 number 3 i. V. m. paragraph 7 and 7a) 2.2.4 other hygienisierende treatment 2.2.4.1 the litigation 2.2.4.2 requirements process testing () to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) 2.2.4.3 process monitoring (article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6) 2.2.4.4 tests of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 number 3 i. V. m. paragraph 7 and 7a) 3 tests of 3.1 seuchen - and sanitary safety process testing (to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) 3.1.1 General requirements of 3.1.2 installations for the aerobic hygienisierenden treatment (thermophilic composting plants)
3.1.2.1 rental composting 3.1.2.2 other composting methods 3.1.3 equipment for the anaerobic hygienisierenden treatment (thermophilic digesters) 3.2 process monitoring (to article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6) 3.3 testing of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 No. 3 i. V. m. paragraph 7 and 7a) 4 methods for testing the seuchen - and sanitary safety 4.1 tracer investigations to determine of the minimum retention time on anaerobic hygienisierenden treatment procedures (thermophilous fermentation) 4.1.1 tracer study with spores of Bacillus globigii 4.1.1.1 preparation 4.1.1.2 implementation Investigating 4.1.1.3 4.1.1.4 detection method minimum retention period 4.1.2 tracer study with lithium 4.1.2.1 preparation 4.1.2.2 inquiry 4.1.2.3 4.1.2.4 detection method minimum retention period 4.2 tests of epidemics hygiene 4.2.1 process test 4.2.1.1 hygienisation test organism and limit 4.2.1.2 deposit samples for aerobic procedure (thermophilic composting) 4.2.1.3
Deposit samples for anaerobic hygienisierende procedure (thermophilous fermentation) 4.2.1.4 detection method 4.2.2 tests the sanitized bio-waste 4.3 testing of the phytosanitary 4.3.1 process testing 4.3.1.1 test organisms and limits 4.3.1.2 test organism Plasmodiophora brassicae 4.3.1.2.1 manufacture of core samples for aerobic hygienisierende treatment procedures (thermophilic composting) 4.3.1.2.2 hygienisation manufacture of core samples for anaerobic treatment procedures (thermophilous fermentation) 4.3.1.2.3 detection of infectivity 4.3.1.3 test organism tomato seeds 4.3.1.3.1 bio test production of the deposit sample 4.3.1.3.2 determination of germination rate by a Biotest 4.3.1.4 test organism of tobacco mosaic virus in aerobic hygienisierenden treatment procedures (thermophilic composting) 4.3.1.4.1 production the deposit samples of 4.3.1.4.2 detection of infectivity 4.3.2 bio test checks the sanitized bio-waste 1 General remarks in this annex are the requirements and the requirements for hygienisierende treatment (systems and processes) and checks the sanitized biowaste described.
Bio-waste of treatment, which supplied not complies with the requirements of the sanitizing (E.g. mesophilic fermentation), is the hygienisierende treatment of bio-waste according to the requirements of this annex in addition perform.
The plant is to conduct and the treatment is to perform to avoid recontamination of the hygienisierend treated materials.
2 sanitiser treatment 2.1 treatment procedures to the sanitizing (to section 2 number 2) hygienisierende treatment of bio-waste is carried out by a) pasteurisation (point 2.2.1), b) aerobic hygienisierende treatment (thermophilic composting) (point 2.2.2), c) anaerobic hygienisierende treatment (thermophilous fermentation) (number 2.2.3) or d) otherwise Hygienisierungsbehandlung (number 2.2.4).
2.2 requirements to the hygienisierende handling 2.2.1 pasteurization may pasteurization before or are performed after an additional, especially biological stabilising treatment (such as mesophilic fermentation).
2.2.1.1 the litigation requirements are before of pasteurization to shred organic waste to a particle size with an edge length (two-dimensional) of each up to 12 mm. The material is to homogenize the heat and must have a water content, which ensures a sufficient heat transfer between and within the particles.
The process control in pasteurization plants must be made for the sanitation of organic waste that a temperature of at least 70 ° C for a continuous period of at least 1 hour acts on all materials.
2.2.1.2 process testing (to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) for pasteurization plants, no process testing referred to in point 3.1 is required; instead pasteurization systems are before the competent authority, if necessary with the assistance of an expert, technically to remove (section 3 paragraph 5 sentence 3). The competent authority issuing an inspection certificate, if it has determined that the pasteurization system meets the requirements of the litigation referred to in point 2.2.1.1 and is equipped with the necessary facilities and equipment, in particular with a) equipment to monitor the temperature, b) devices for continuous recording of measurement results and c) a reasonable safety system to prevent insufficient heating.
2.2.1.3 process monitoring (to article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6) process monitoring is performed according to the requirements of point 3.2.
2.2.1.4 tests of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 No. 3 i. V. m. paragraph 7 and 7a) the tests of sanitized organic waste shall be according to the specifications of point 3.3 and the methods referred to in point 4.2.2 (epidemics hygiene) and number 4.3.2 (phytosanitary).
2.2.2 aerobic hygienisierende treatment (thermophilic composting) 2.2.2.1 requirements to the litigation process control in composting plants must thus be made for deep cleaning of organic waste, a thermophile temperature range and a high biological activity in low moisture and nutrient conditions and an optimal structure and airflow are guaranteed over several weeks. The water content should be at least 40% and the pH to 7 are. In the course of aerobic hygienisierenden treatment a temperature of at least 55 ° C over a possible continuous period of 2 weeks, 60 ° c for 6 days or 65 ° c must interact over 3 days all the rotting material.
2.2.2.2 process testing (to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) for composting facilities to the sanitizing process testing is according to the 3.1.1 and 3.1.2 numbers perform.
To the use of test organisms (test and indicator germs) and the verification of their destruction or inactivation following methods to be applied are: a) for epidemics hygiene methods in accordance with number 4.2.1 (except number 4.2.1.3) and b) for the phytosanitary the methods referred to in paragraph 4.3.1 (except number 4.3.1.2.2).
2.2.2.3 process monitoring (to article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6) process monitoring is performed according to the requirements of point 3.2.
2.2.2.4 tests of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 No. 3 i. V. m. paragraph 7 and 7a) the tests of sanitized organic waste shall be according to the specifications of point 3.3 and the methods referred to in point 4.2.2 (epidemics hygiene) and number 4.3.2 (phytosanitary).
2.2.3 anaerobic hygienisierende treatment (thermophilous fermentation) 2.2.3.1 requirements to the litigation process control in fermentation plants must to be made for deep cleaning of organic waste, that through the continuous period of the minimum dwell time the treatment temperature in the thermophilic range (at least 50 ° C) acts on all the material. This which may be used in assessing passing process (s. number 2.2.3.3) used technically given or proven minimum retention period (s. number 2.2.3.2) and the used treatment temperature will not fall below.
2.2.3.2 determine of the minimum retention period if the minimum dwell time in the fermenter is not technically given by means of a hydraulic shut-off within the loading and sampling intervals, must be by a tracer study according to a method referred to in point 4.1 prior to process testing (see number 2.2.3.3) must be demonstrated.
The tracer study determines the amount of time the fermentation plant to the sanitation which have all parts of the substrate as the shortest time of stay in the fermenter (solid and liquid). Here, the substrate to vergärende before adding in the fermenter is marked with indicators (tracer). The minimum retention time of vergärenden material in the fermenter is the period of time determined until the final rehearsal without finding before initial proof of the tracer.
Pending the results of the tracer study the minimum residence time calculated by the turbine manufacturers and planners is reached at the plant. So the minimum retention period is reached, not permanently exceeded the maximum daily amount of input identified by the plant manufacturers and planners after reaching the target level in the fermenters relevant to the sanitizing. A calculation is not available, it is in agreement with the competent authority to create, if necessary with the assistance of an expert.
2.2.3.3 process testing (to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) for fermentation plants for the sanitizing process testing is according to the 3.1.1 and 3.1.3 numbers perform.
An in the thermophilic temperature range (at least 50 ° C) required treatment temperature is to use when assessing process. The process check is with the technically specified or proven minimum delay time (see number 2.2.3.2) perform.
To the use of test organisms (test and indicator germs) and the verification of their destruction or inactivation following methods to be applied are: a) for epidemics hygiene the methods referred to in number 4.2.1 (except number 4.2.1.2), as well as b) for the phytosanitary the methods referred to in number (except for test organism of tobacco mosaic virus in accordance with letter c) 4.3.1.1 4.3.1.2 (except number 4.3.1.2.1) and number 4.3.1.3.
It is not select the process test, with a higher temperature of treatment or prolonged minimum delay time to repeat.
2.2.3.4 process control (to article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6) process monitoring is performed according to the requirements of point 3.2.
2.2.3.5 tests of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 No. 3 i. V. m. paragraph 7 and 7a) the tests of sanitized organic waste shall be according to the specifications of point 3.3 and the methods referred to in point 4.2.2 (epidemics hygiene) and number 4.3.2 (phytosanitary).
2.2.4 is otherwise hygienisierende treatment for an otherwise hygienisierendes treatment procedures, if necessary with the assistance of an expert, to demonstrate equivalent efficacy of sanitizing (§ 3 (3) sentence 4) measured to the requirements of this annex.
2.2.4.1 requirements for process management
The requirements on the process control for the hygienisierenden treatment of bio-waste may with the assistance of an expert, in consultation with the competent authority, to determine and to describe that achieves an equivalent Hygienisierungsniveau.
2.2.4.2 process testing (to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) the process audit requirements are in agreement with the competent authority, to determine and to describe that a Hygienisierungsniveau equivalent is achieved, taking into account the requirements of 3.1.1 number as well as the methods referred to in 4.2.1 (epidemics hygiene) and number 4.3.1 (phytosanitary) if necessary with the assistance of an expert.
2.2.4.3 process monitoring (to article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6) the requirements for process monitoring are in agreement with the competent authority, to determine and to describe that a Hygienisierungsniveau equivalent is achieved, taking into account the requirements of point 3.2 if necessary with the assistance of an expert.
2.2.4.4 tests of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 No. 3 i. V. m. paragraph 7 and 7a) the tests of sanitized organic waste shall be according to the specifications of point 3.3 and the methods referred to in point 4.2.2 (epidemics hygiene) and number 4.3.2 (phytosanitary).
3 tests of the safety of seuchen - and sanitary hygienic safety of bio-waste is determined with the help of a) process assessment in accordance with article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5 and in accordance with the descriptions in paragraph 3.1, b) process monitoring in accordance with article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6 and in accordance with the descriptions in section 3.2 and c) tests of sanitized organic waste in accordance with article 3, paragraph 4, sentence 1 No. 3 i. V. m. Paragraph 7 and 7a and in accordance with the descriptions in point 3.3.
The seuchen - and sanitary testing should be possible at the same time.
The treated organic waste are to be classified only as hygienically safe, if passed all tests referred to in paragraphs 3.1 to 3.3.
3.1 process testing (to article 3, paragraph 4, sentence 1 number 1 i. V. m. paragraph 5) 3.1.1 General requirements the process audit is an examination of the individual treatment plant to the deep cleaning, making each unique new direction of the system and significant modification of the procedure is. This determines the effectiveness of the Hygienisierungsverfahrens. This, with the bio-waste, seuchen - and phytohygienisch-related test or indicator organisms are introduced into the system; based on studies of the treated material is then checked, whether through deep cleaning the test organisms have been killed or inactivated.
For an otherwise hygienisierende treatment (2.2.4), the requirements for the process review in consultation with the competent authority, to determine and to describe that a Hygienisierungsniveau equivalent is achieved, taking into account the provisions of this section, as well as the methods referred to in 4.2.1 (epidemics hygiene) and number 4.3.1 (phytosanitary) may with the assistance of an expert.
For the process of testing the methods (sampling, preparation, investigations, and to observe a maximum limits) at of epidemics hygiene in accordance with paragraph 4.2.1 and the phytosanitary referred to in 4.3.1, and in accordance with the descriptions detailed below for the respective plant are (see number 3.1.2 and 3.1.3) to apply (section 3 paragraph 4 sentence 2).
The process audit is successfully completed if the limits according to 4.2.1.1 (epidemics hygiene) and number 4.3.1.1 (phytosanitary) through the two successive rounds of investigation each not be exceeded after the procedure relevant to the sanitizing.
3.1.2 includes two temporally separate investigation course at a minimum of 3 months, the aerobic hygienisierenden treatment (thermophilic composting plants) the process of testing equipment which one to take place has in the winter.
The test organisms are inserted in characteristic Rotte areas or the process sections representative of the thermal inactivation of test organisms and examined after removal on survivor or infectious test organisms.
3.1.2.1 rental composting every course of study be examined 60 samples, consisting of 24 samples on the examination of epidemics hygiene and 36 samples on the inspection of the phytosanitary. The number of samples is determined as follows: a) at of epidemics hygiene test 1 test organism is (see number 4.2.1) brought in double samples in three different zones of Rotte (edge, core and base area), as well as four different positions of the rent.
(b) in the case of the inspection of the phytosanitary 3 test organisms are (see number 4.3.1) introduced as a single specimen in three different zones of Rotte (edge, core and base area), as well as four different positions of the rent.
The samples on the edge may be covered with 10 cm of composting good. The samples remain up to the end of the test in the respective areas.
For small plants with an annual capacity of up to 3 000 tonnes of material, only a reduced scope of analysis with a halving of the samples to be examined is necessary. Here, the test organisms only in two different places of rent be introduced.
3.1.2.2 other composting methods for each course of study be investigated a total of 60 samples, which 24 samples for the examination of epidemics hygiene (1 test organism, s number 4.2.1) and 36 samples on the test of phytosanitary (3 test organisms, see number 4.3.1) accounts for. The test organisms are inserted in characteristic areas of the rotting body or smuggled in dynamic processes in appropriate sample containers with the flow of material through the usual Rotte and process. The inserted sample container must exhibit a sufficient perforation so that the fabric implementation conditions within the sample container correspond to those of the composting process to be tested to the sanitizing.
Dynamic process is to ensure that all test organisms are admitted time as evenly as possible throughout the entire installation process so that they are as homogeneously distributed in the composting unit. In addition, the shape of the used sample containers must ensure to match the material to kompostierenden with regard to the behaviour in the material flow and the dwell time.
Unless the specific equipment not limited the size of the sample container (such as free passes for worm gears, etc.), a total of 12 test tube in the composting unit inserted (pass through); (each sample container contains a) a test organism in double samples for testing of epidemics hygiene (see number 4.2.1) and b) three test organisms as a single sample for checking the phytosanitary (s. number 4.3.1).
The input (challenges) large sample container is not possible, the samples on a correspondingly larger number of smaller sample container must be distributed.
For small plants with an annual capacity of up to 3 000 tonnes of material, only a reduced scope of analysis with a halving of the samples to be examined is necessary. Are introduced only 6 sample container instead of 12 and smuggled through.
3.1.3 equipment for anaerobic hygienisierenden treatment (thermophilous fermentation plants) the process review includes two temporally separate investigation course at a minimum of 3 months.
Each course of study, a total of 24 single samples are tested which 8 samples on the test of epidemics hygiene and 16 samples on the test of phytosanitary. The number of samples is determined as follows: a) at of epidemics hygiene test 1 test organism is (see number 4.2.1) brought in double samples and four different positions in the fermenter (when the fermenters in vertical and the fermenters in the horizontal direction).
(b) in the case of the inspection of the phytosanitary 2 test organisms are (see number 4.3.1 with the exception of the tobacco mosaic virus) brought in double samples and four different positions in the fermenter (when the fermenters in vertical and the fermenters in the horizontal direction).
For small plants with an annual capacity of up to 3 000 tonnes of material, only a reduced scope of analysis with a halving of the samples to be examined is necessary. Here, the test organisms only in two different places are inserted into the fermenter.
The test organisms are for the technically specified or proven minimum retention period (see number 2.2.3.2) inserted into the fermenter and examined after removal.
For the verification of the process, appropriate openings in the fermentation tanks must be present for the deposit and collection of samples.
3.2 process monitoring (to article 3, paragraph 4, sentence 1 number 2 i. V. m. paragraph 6) process monitoring is a continuous testing and recording the temperature during treatment to the sanitizing. Hereby, it is proven whether the required temperature for the sanitizing and the necessary duration of exposure is respected during treatment.
For an otherwise hygienisierende treatment (2.2.4), the requirements for process monitoring in coordination with the competent authority, to determine and to describe that a Hygienisierungsniveau equivalent is achieved, taking into account the provisions of this section may be with the assistance of an expert.
Is measured in a closed composting plant to the sanitizing the temperature in the exhaust air stream of compost rent and recorded (section 3 paragraph 6 sentence 3), is to determine the treatment temperature on a plant-specific correction factor compared to the direct temperature measurement in the Rotte. The plant-specific correction factor can be checked regularly by parallel direct temperature measurements in the rotting material. For temperature measurement in the exhaust stream, the requirements set in agreement with the competent authority, may with the assistance of an expert.
The temperature measurements are to make in representative zones of process sections relevant for the sanitizing or parts.
The process audit is successfully completed when they were held for each process temperature and duration of exposure (see paragraph 2.2.1.1, 2.2.2.1, 2.2.3.1 and 2.2.4.1) in the hygienisierenden treatment of the material.
3.3 testing of the sanitized bio-waste (to article 3, paragraph 4, sentence 1 No. 3 i. V. m. paragraph 7 and 7a) tests the sanitized bio-waste must be regular investigations of materials after treatment to the sanitation on pathogens, viable seeds and shoots-capable parts of plants.
The tests of sanitized organic waste are carried out according to the Hygienisierungsbehandlung (see number 2) on the duty-finished material. In each examination of the sanitized bio-waste, a sample of epidemics hygiene and the phytosanitary is each to investigate.
For the tests, methods (sampling, preparation, investigations, and to observe a maximum limits) at of epidemics hygiene in accordance with paragraph 4.2.2 and the phytosanitary referred to in point 4.3.2 shall apply (section 3 paragraph 4 sentence 2).
The tests of sanitized organic waste are successfully completed, if exceed the limits referred to in point 4.2.2 last paragraph (epidemics hygiene) and number 4.3.2 last paragraph (phytosanitary) in any of the samples taken.
4 methods for testing the seuchen - and sanitary safety 4.1 tracer investigations to determine of the minimum dwell time in anaerobic hygienisierenden treatment procedures (thermophilous fermentation) the sanitiser assess action of anaerobic treatment process, knowledge of the minimum dwell time of waste suspension in the fermenter of importance is. The minimum retention period must be determined, is this a tracer study conducted (see number 2.2.3.2). When the tracer study, the suspension of waste before it enters into the fermenter is marked with indicators (tracers) and recorded their first appearance at the outlet.
The tracer study in anaerobic treatment plants to the sanitizing of biodegradable waste are biological tracer with the spores of Bacillus globigii (see number 4.1.1) or chemical tracer with lithium (see number 4.1.2) suitable.
4.1.1 tracer study with spores of Bacillus globigii as tracer biological spores of Bacillus globigii used. This test bacteria spores do not occur naturally in the biological substrates, they are apathogen for man and animal, survive the effects of process in anaerobic treatment plants and are easily detectable.
4.1.1.1 preparation of necessary materials and reagents - Tryptone glucose broth (TGB), for the production of the vaccine culture of Bacillus globigii spores: yeast extract: 2,5 g Tryptone: 5,0 g, glucose: 1,0 g, water (distilled): 1 000 ml;-yeast extract agar (MYA), for the production of Bacillus globigii spores: Peptone meat: 10.0 g yeast extract: 2,0 g, manganese sulphate monohydrate: 0.04 g agar: 15 g, water (distilled): 1 000 ml;-Bacillus globigii tribal culture, for the production of the Bacillus globigii tribal cultures spore suspension : Bacillus globigii (DSM1 No. 675 [BAC. Atrophaeus]) or Bacillus globigii (DSM1) No.. 2277 [BAC. Atrophaeus]) or Bacillus globigii (master collection of the University Hohenheim2).
-Centrifuge with an acceleration of 10 000 g. sample preparation Tryptone – glucose broth (TGB): the broth is given in portions of 10 or 100 ml in a test tube. It is sterilized in the autoclave. After sterilization, the pH of the medium 7.2 (± 0.2), measured at 20 ° C, must be.
Yeast extract agar (MYA): The agar is given in Roux bottles or Petri dishes. It is sterilized in the autoclave. After sterilization, the pH of the medium 7.0 (± 0.2), measured at 20 ° C, must be.
Bacillus globigii tribal cultures: the Bacillus globigii tribal cultures (glycerol culture, storage temperature - 80 ° C) are thawed and incubated in Tryptone – glucose broth (TGB) at 37 ° C for 24 hours.
From the TGB bouillon 6 ml onto MYA plates; the supernatant is pipetted. MYA plates are at 37 (± 1) ° C incubation. After the third day of incubation, the condition of the crops with the help of a spore staining (E.g. racket staining) is assessed. There is then a further incubation of MYA plates at 30 ° C over a period of 7 to 10 days. Then, the colonies are washed off by MYA plates with 3 ml of sterile distilled water (aqua dest.).
The winning spore suspension is centrifuged (3 000 rpm for 10 minutes), projecting is discarded and the Pellet is with aqua dest. resuspended.
The suspension will first determine of the number of spores at 75 (± 1) ° C heat for 10 minutes, then the number of spores per milliliter is determined with the Koch´schen surface process suspension.
4.1.1.2 conducting of the investigation of biological tracer is added once evenly the fermenter in the form of a spore suspension during each loading interval. It is a loading charge added so much suspension of spores, that there are at least 106 spores per gram FERM enter content.
The concentration of Bacillus globigii spores in the suspension must be checked.
After the feeding of the spore suspension is the sampling (single sample of approx. 1 kg) in the discharge as long as the tracer was first detected in a sample, at least a) every hour including the 24 hour, b) that following every two hours including the 36 hour, c) following every 4 hours on it including the 48th hour, d) that following every 6 hours.
4.1.1.3 detection method the samples to be examined are off to the Predilution 20 g 180 ml sodium chloride (0.9% saline) weighed and mixed 20 hours at 4 ° C on the shaker. After a sufficient mixing NaCl solution is pipetted into each well per 1 ml of sample in geometric series to the degree of dilution 10-8 in 9 ml each. Then, each 0.1 ml of each dilution step on two parallel standard I agar plates are pipetted into each well and evenly with a from-annealed wire spatula (incubation 37 ° C / 24 hours).
Only those colonies, which show a typical orange red growth be counted out on agar plates.
4.1.1.4 minimum dwell time is the minimum retention period the period of time between the addition of Bacillus globigii spore suspension and the last sample no findings before the first evidence of the biological tracer in the discharge of the fermenter.
4.1.2 tracer survey preparation first is determination of lithium base load in the waste suspension with lithium 4.1.2.1 to determine the natural lithium base load in the waste suspension. A representative sample of the discharge of the fermenter is taken every day for at least 5 days prior to the exam and the lithium content was determined. Depending on the organic waste composition is the base load of lithium generally between 1 and 5 mg per kg of dry matter.
Tracer materials required: Lithium hydroxide monohydrate 4.1.2.2 carrying out the inquiry for the investigation is related to set the contents FERM boarding (full mix) the lithium concentration of 50 mg / kg of dry matter. The amount of lithium is dependent on the fermenter enter volume of the fermentation plant to validate to the sanitizing. The tracer is added the fermenter in dissolved form evenly during each loading interval.
From this suspension of lithium, a sample is pending the result to be kept.
Sampling (single sample of approx. 1 kg) in the discharge takes place as long as the tracer is detected for the first time in a sample after the feeding of the tracer (lithium concentration > base load), and at least a) every hour including the 24 hour, b) following every 2 hours on it including the 36 hour, c) that following every 4 hours including the 48th hour , d) that following every 6 hours.
4.1.2.3 detection method for determining the concentration of lithium are the sample analysed according to DIN EN ISO 11885:20093.
4.1.2.4 minimum retention period
The minimum retention period is the period of time between the addition of the lithium tracer and the last sample no concentration increase prior to the initial detection of the tracer in the discharge of the fermenter. The tracer is detected, if the detected concentration of lithium exceeds the calculated base load to the double standard deviation, which is determined in the samples taken in accordance with paragraph 4.1.2.1.
4.2 tests of 4.2.1 health hygiene process test 4.2.1.1 test organism and limit is the process audit at of epidemics hygiene with the test germ carried salmonella senftenberg W775(H2S-negativ).
The process audit is successfully completed at of epidemics hygiene, when in the two successive rounds of investigation after the relevant to the sanitizing procedure in any sample salmonella are detectable.
4.2.1.2 deposit for aerobic hygienisierende procedure (thermophilic composting) the test germ salmonella senftenberg W775 (H2S negative) is in standard I broth at 37 ° C over 18 to 24 hours incubated. The generated germ suspension should contain a micro-organism concentration of at least 107 to 108 cfu/ml. The concentration is by comparison with a standard (E.g., McFarland) or the surface procedures or MPN procedure (most probable number) to determine.
In the composting to the sanitizing per sample fresh, homogenized and crushed organic waste material from the system to be checked with 25 ml of this germ suspension soaked approx. 225 g and then packed in sterile onion or plastic bags. The deposit of samples in the composting is carried out either in this form or in roughly perforated stable sample containers that are suitable for the particular process. After the procedure section relevant to the sanitizing is completed, the sample container is removed, and each 50 g of the homogenized contents of samples saeckchens in 450 ml buffered Peptone water with Novobiocin for 30 minutes at 4 ° C slowly shaking (150 rpm) and then 22 (± 2) hours at 36 (± 2) ° C incubated. The obtained suspension solution is used for the identification of salmonella.
4.2.1.3 deposit samples for anaerobic hygienisierende procedure (thermophilous fermentation) the bacterial suspension with the test germ salmonella senftenberg W775 (H2S negative) is made as described in number 1 paragraph 4.2.1.2.
In digesters to the sanitation, each 1 ml of bacterial suspension of salmonella senftenberg W775 is introduced with Diffusionskeimträgern4 (H2S negative) in the process. The diffusion germs be filled except with 1 ml of bacterial suspension with 9 ml fermentation residue and in that for the thermal inactivation minimum residence time identified relevant sections of process or plant components for the (see point 4.1) and Hygienisierungstemperatur. After the procedure is completed, the respective total of diffusion (10 ml) germs in 90 ml of buffered Peptone water with Novobiocin (pre-enrichment) is given, briefly shaking (150 rpm) and 22 (± 2) hours at 36 (± 2) ° C incubated. The obtained suspension solution is used for the identification of salmonella.
4.2.1.4 detection method existing salmonella are identified with the suspension solutions, which have been manufactured according to the methods described above (see number 4.2.1.2 and 4.2.1.3). To do this, each 0.1 ml from the well mixed before enrichment in 10 ml of enrichment broth are after Rappaport at 36 (± 2) ° C and at 42 (± 1) ° C (± 2) 22 hours ago incubated. Then parallel smears on Xylose-lysine Desoxycholat (XLD) agar and a further salmonella differential agar with the detection ability of biochemical properties other than XLD agar are created. Suspect salmonella colonies are on nutrient agar via vaccinate and at 36 (± 2) ° C (± 2) 22 hours ago incubated. The identification is made on the basis of the body and the scourge antigens (O - and H-antigens) biochemically or serology.
Control of survivability (MOH) of the test strain, four control samples manufactured parallel to assessing process. This control samples are not brought into the Hygienisierungsverfahren, but stored during the exam period in humid sand (such as bucket with quartz sand, humidification with deionized water) at room temperature (20 to 25 ° C), and worked after discontinuation of the trial of process. At least three of the four control samples should provide positive salmonella results; otherwise, the MOH of the test strain is not regarded as sufficient.
4.2.2 tests the sanitized bio-waste for inspecting the sanitized bio-waste at of epidemics hygiene are from a well mixed aggregate sample (approx. 3 kg) each 50 g material according to the method specified above (see number 4.2.1.2) examined for the presence of salmonella. The Sammelmischprobe is composed of at least five different partial samples in a batch of hygienisierend treated, in accordance with paragraph 3.3 material to be examined together.
Inspecting the sanitized bio-waste is successfully completed at of epidemics hygiene, if there is no salmonella detectable in each 50 g of collecting samples.
4.3 testing of the phytosanitary 4.3.1 process testing 4.3.1.1 test organisms and limits the variety of Phytopathogenen and plant seeds, which are the raw material of bio-waste treatment plants, be following control or indicator organisms in process checks in the phytosanitary used: a) Plasmodiophora brassicae (cabbage hernia) with a week-long heat tolerance of 50 ° C, limit in the Biotest: infestation index ≤ 0,5 per test area b) tomato seeds, limit in the Biotest: ≤ 2% viable seeds per test area , c) in addition to aerobic hygienisierender treatment (thermophilic composting) in accordance with point 2.2.2: tobacco mosaic virus (TMV), limit in the Biotest: ≤ 4% Restinfektiosität (relative to value the positive control) each test area.
The process audit is successfully completed in the phytosanitary, if in the two successive rounds of investigation each after the relevant to the sanitizing procedure in samples per test range the specified limits - the parameters of Plasmodiophora brassicae and not exceeded tomato seeds as well as - exceeding the parameters of tobacco mosaic virus by not more than up to 30%.
4.3.1.2 test organism Plasmodiophora brassicae is according to the following described methodology carried out the process audit in the phytosanitary with the test organism Plasmodiophora brassicae.
4.3.1.2.1 manufacture of core samples for aerobic hygienisierende treatment procedures (thermophilic composting) the bile material (infectious material to the pathogen Plasmodiophora brassicae) is to manufacture of the core samples at - 25 ° C frozen. It is proven infectious to the pathogen Plasmodiophora to use heat-tolerant materials of bile brassicae infected cabbage plants. Heat tolerance is established, if the bile material during incubation at 50 ° C for 7 days a high infectivity (infestation level ≥ 2) has.
Each sample used in the composting process to the sanitizing contains 30 g bile material, 430 g floor, and 200 g of the compost raw material. This corresponds to a ratio of about 5% bile material 65% floor and 30% compost. The individual samples are intensive mixed with rotting resistant bags (mesh 1 x 1 mm) filled; This is to ensure that none of the sample in the surrounding compost is played out.
Accordingly manufactured control samples be stored during the trial period in moist sterilized sand at room temperature.
Making the deposit samples for anaerobic hygienisierende treatment procedures (thermophilous fermentation) for the material of the bile to be used (infectious material with the pathogen Plasmodiophora brassicae) is considered number 4.3.1.2.1 paragraph 1 according to 4.3.1.2.2.
In fermentation plants, 30 g bile material in gauze bags become the sanitizing (mesh size 1 x 1 mm) brought in for the thermal inactivation of relevant sections of the process or plant parts.
Accordingly manufactured control samples be stored during the trial period in moist sterilized sand at room temperature.
4.3.1.2.3 detection of infectivity by a bio test existing residual infection of Plasmodiophora brassicae in the deposit samples is determined by the following test.
Needed materials - mixing tub, - (1 000 ml) measuring cup - plastic pots (13 x 13 x 13 cm, approx. 1 l), matching coasters, - certified seed of Sarepta mustard (Brassica juncea), - substrate absorber, sand, grit 0,8-1,2 mm (such as red sandstone with good buffering capacity, pH ~ 6.5), - white peat (pH approx. 3.5), - pH-meter, - disposable gloves (for each sample of a few) - water soluble fertilizer (solid or liquid).
Sample preparation to recycling of the tested Hygienisierungsverfahren are the deposit samples carefully crushed with the pathogen Plasmodiophora brassicae and with a mixture of sand and peat (steamed for 5 hours at 80 ° C) on a volume of 1 000 ml filled up and well homogenized.
Since the pH of a strong influence the infectivity of Plasmodiophora brassicae exerts on (optimum: pH 6.0 ± 0.2), the pH value of the produced mixture of substrate is to check and correct if necessary by increasing the proportion of peat.
Bio test
13 x 13 x 13 cm-large plastic pots are used as experimental vessels. For each the isolated pathogen sample, which was filled with the mixture of sand and peat per 1 000 ml, a vessel with 16 proof plants applied Sarepta mustard (Brassica juncea); There are early seed plants (1 leaf Leafage) a handle in each vessel. The Biotest is situated as a randomized trial in a climate Chamber at 6 000 to 9 000 lux and a temperature of at least 20 ° C or in the greenhouse. The plants are fertilized once a week from the third week. The vegetation period of bio tests up to the note taking of evidence plants is 4 to 5 weeks.
One counts the number of infested plants after the completion of the bio test and on the other the root gall formation measured according to a Boniturskala from 0 to 3: attack class description of symptoms 0 no visible symptoms 1 light bile formation of main and secondary roots 2 medium-sized bile formation at main - and secondary roots 3 strong formation of bile on the entire root system Boniturnoten for the infestation of individual plants are evaluation of Boniturnoten for each pathogen sample (repetition) (= KL infestation class) summarized in the infestation index according to the following formula : The infestation index for a range of test results from the arithmetic mean of the infestation index of all repetitions (pathogen samples) of the respective test area: the infestation index per test area is ≤ 0.5, the exam is passed.
4.3.1.3 test organism tomato seeds the process audit in the phytosanitary with the test organism tomato seeds is carried out according to the following described methodology.
The following materials are required for the manufacture of the core sample and determining the germination rate a bio test:-plastic petri dishes with lid (Ø 9 cm), - circular filter paper, seeds of tomato (Lycopersicon lycopersicum [L.] Karsten ex Farw.), type of Saint-Pierre (synonym: San Pedro).
4.3.1.3.1 making the deposit sample about 1 g or 400 seeds of tomato (Lycopersicon lycopersicum [L.] Karsten ex Farw.) the variety of Saint-Pierre (synonym: San Pedro) are filled in a little bag from unverrottbarem gauze fabric (mesh size 1 x 1 mm) and distributed prior to closure on the entire surface of the gauze, to achieve a minimum layer thickness of tomato seeds. The germination of tomato seeds must be determined before the examinations. Only seeds with a minimum germination capacity of 90% must be used for testing.
4.3.1.3.2 the test organism from the core samples taken from determination of germination rate a bio test after which will study and immediately subjected to a germination test.
The tomato seeds are removed from the deposit sample bio test and 200 seeds are counted. The remaining seeds are 1 to 2 days under living conditions (20 to 50% rel. hum. Humidity, about 20 ° C) return dry, airtight and kept (rear grab sample) for any repetitions of determining germination capacity in the refrigerator. Counted seeds be in a clean condition, if necessary washed off, designed for determining germination capacity, such as 4 x 50 seeds on 4 layers of damp filter paper in covered Petri dishes with a diameter of 9 cm at 25 ° C and exposure in a suitable room or Cabinet. 7 every seven days will be germinated tomato seeds so long counted, until no further seeds germinate. The seed, in which the root or the stem is visible resigned is considered to be germinated. No seeds germinated after 21 days the germination test is completed.
Evaluation of results the total number of germinated seeds is determined and specified as a percentage of the seeds used in the tested aliquot (200 seeds). The germination of tomato seeds in a field stems from the arithmetic mean of the germination rates of all repetitions (pathogen samples) of the test area.
4.3.1.4 test organism of tobacco mosaic virus in aerobic hygienisierenden treatment procedures (thermophilic composting) the process audit in the phytosanitary with the test organism of tobacco mosaic virus is carried by the following represented method.
The following materials and reagents are required for the production of core samples and the detection by a bio test: - plastic pots with a volume of 500 ml with base perforation and lower shells, water soluble fertilizer, - seed of tobacco (Nicotiana tabacum "Samsun"), - seed tobacco (Nicotiana glutinosa L.), - 0 (EE0) standard soil as planting substrate, mortar and pestle, - carborundum-bentonite mixture (ratio 1:1), phosphate buffer after Sörensen (pH 7) or a corresponding commercially available product, - TMV-containing suspension (plant juice from TMV infected tobacco plants) , - Filtration gauze, - commercial cotton swab, - lockable glass or plastic vessels, storage vessels and humidity trays.
4.3.1.4.1 of deposit samples the propagation of the virus is produced in tobacco plants (Nicotiana tabacum "Samsun"), where it is systemically spreading. To do this, the tobacco plants at 18 to 22 ° C under greenhouse conditions used up to the 5-leaf stage. For inoculation, sparsely powdered up 2 or 3 lower leaves with a mixture of carborundum and bentonite (1:1) and the TMV-containing suspension (plant juice from TMV infected tobacco plants) in 0.05 mol / l phosphate buffer according to Sörensen, or according to (pH 7) applied to the pollinated leaves. 2 to 3 weeks after inoculation then virus-containing leaves with mosaic-like discolorations can be used for the investigations.
Each sample is injected into the composting process to the sanitizing contains 10 g TMV infected tobacco leaves (Nicotiana tabacum "Samsun"), which are filled in a rotting resistant muslin bag (1 x 1 mm mesh size). So the composting conditions can affect the TMV infected tobacco leaves, the muslin bag of the core sample is completely to surround with compost raw material.
There are 10 g with TMV infected tobacco leaves positive controls (Nicotiana tabacum "Samsun") to produce same batch, stored at - 18 ° C.
4.3.1.4.2 detection of infectivity by a bio test the inactivation of the pathogen samples through the Hygienisierungsprozess of thermophilic composting is examined by a bio test according to the following described method.
Sample preparation is free after completion of the hygienisierenden procedure step (such as withdrawal after completing the examination of process of a thermophilic composting plant) of any not rotted coarse components the TMV-Exciter probe. With the addition of 30 ml of phosphate buffer after Sörensen or equivalent (0,05 mol / l, pH 7) the sample in a mortar is crushed. The sample suspension is given to the filter gauze and pressed. The sample extract is transferred in a lockable glass or plastic container.
With the accompanying positive control samples are handled in the same way.
Bio test as evidence of the infection are the extracts from the samples and the controls applied on the leaves of the test plant (Nicotiana glutinosa L.). The bio test is performed on proof plants located in the 6 - 8 leaf stage.
A total of 12 proof plants are needed for the inoculation of 12 TMV, isolated pathogen samples per inspection area test four samples at four plants.
On the plants of proof of, the tip of the vegetation and the lower older leaves be removed so that four fully formed leaves for the inoculation of the plants remain. For better comparability with local lesions on plants with leaves of different sizes and different ages, the Latin square as experimental arrangement is to choose. The prerequisite for this is the same number of TMV samples, test plants and leaves. The three characteristic areas of the rotting body in each fourfold repetition are checked when assessing process. The following schema shows the experimental setup the half leaf method taking into account the positive control sample (P) for the four examiners TMV samples (A, B, C, D) a test area: plant 1, plant 2 plant 3 plant 4 sheet position part (from the direction of blade tip) part (from the direction of blade tip) part (from the direction of blade tip) part (from the direction of blade tip) left right left right left right left right 1 sheet A P P D C P P B 2. sheet B P P A D P P C 3. Sheet C P P B A P P D 4 sheet D P P C B P P A that can leaves in terms of identified the performed treatments on the underside of the leaves with a permanent marker. First, always the test sample is applied, and then the control sample.
Then, the leaves of plants of detection with a mixture of carborundum and bentonite (1:1) are sparsely powdered. The sample and control extracts applied with a cotton swab on the leaves, whereby the pollinated leaf halves with the extract be painted twice evenly with a slight pressure and hand movements, ranging from the midvein to the edge of the page. It supported the blade with one hand by the underside of the leaves here.
Immediately after the treatment, the tobacco leaves directly on the Scion cut off and the adherent carborundum / bentonite remains of the leaf surface with tap water (spray or shower) completely removed. For the incubation the treated leaves are placed in a container filled with water or placed in corresponding humidity trays. The connection provided the treated leaves up to impact education in a climate Chamber or an air-conditioned greenhouse at 22 to 25 ° C. The treated leaves daily for 16 hours will be highlighted during the incubation period (exposure at least 2 000 lux).
No later than 5 days after inoculation are clearly to identify the sources of infection in the form of necrotic local lesions. This is small round spots of 2 to 3 mm in diameter, whose Zentren consist of dead tissue.
Evaluation of the results of the assessment shown lesions of each part will be counted separately. The evaluation is done by addition of lesions of the respective four leaf halves, which are respectively have been inoculated with the sample and control solution. The Restinfektiosität of the pathogen samples is expressed as a percentage in relation to the positive control.
Summarizes the relative residual infection on four inoculated tobacco leaves according to the following formula for each pathogen sample (repeat): inoculated sheet of the first plant B2 = B1 = inoculated leaf of the second plant of B3 = inoculated leaf of the third plant B4 = inoculated leaf of the fourth plant LE = lesion number of excitation test LK = number of lesion of the positive control sample that results in residual infection [relative value] of the pathogen of tobacco mosaic virus for a test range from the arithmetic mean of the relative residual infections of all repetitions (pathogen samples) of the respective test area : The residual infection [relative value] per test area is ≤ 4%, the exam is passed.
4.3.2 is testing the sanitized bio-waste in the examination of the sanitized bio-waste in the phytosanitary germinable seed content and material treated with the cultivation method determined shoots enabled parts of plants in the hygienisierend.
The test is performed with material from a well mixed aggregate sample (approx. 3 kg). The Sammelmischprobe is composed of at least five different partial samples in a batch of hygienisierend treated, in accordance with paragraph 3.3 material to be examined together.
To determine the volumetric weight of sample preparation and the salt content of the test substrate. Compost is the original sample < 10 mm screened. Too wet and not screen enabled composts are pre-dried (air drying). Pasteurized materials and liquid digestate are unscreened and used as liquid test substrate.
Required materials - plastic bowls with base perforation or equivalent test containers, pouring mats, - needle perforated film, - appropriate mixed substrate (E.g. weakly decomposed bog peat with approx. 4 g carbonate calcium per litre, which is free of germinable seeds and shoots-capable parts of plants).
Carrying out 3 l of sifted (FS