Chapter 1	Scope and definitions
Chapter 2	Use of certain animals for tests
Chapter 3	Reuse of test animals
Chapter 4	Journal Pre-Journal
Chapter 5	Information, labelling and identification of dogs, cats and non-human primates ;
Chapter 6	Phrase and resettlement of laboratory animals
Chapter 7	General provisions on care and disbarding
Chapter 8	The places of life
Chapter 9	The stables rooms
Chapter 10	Forsisanitation plant
Chapter 11	Transport and killing of laboratory animals
Chapter 12	The care of the seafarers, the care and the others.
Chapter 13	Veterinary advice, supervision, control, etc.
Chapter 14	Qualification requirements for persons dealing with experimental animals
Chapter 15	Application for authorisation for animal testing
Chapter 16	Permission for breeding and delivery of test animals and animal tests
Chapter 17	Subsequent Evaluation
Chapter 18	Special provisions for breeders and other vendors of laboratory animals
Chapter 19	Animal welfare body
Chapter 20	Final provisions
Chapter 21	Punishment, plining and revocation of authorisations
Chapter 22	Entry into force, transitional provisions, etc.
Appendix 1	Animals covered by section 10 (4). 1
Appendix 2	Passing and the stalling of laboratory animals
Appendix 3	Methods for the killing of animals
Appendix 4	Classification of Attempting for Workload

Publication of animal testing 1)

Purses of § 3 a, section 9 (4). Paragraph 1, section 16, paragraph 1. 2, and section 18 of the animal testing law, cf. Law Order no. 1306 of 23. November 2007, as amended by law no. 612 of 14. In June 2011, and after obtained the opinion of the Animal test subject, the authorisation shall be determined :

Chapter 1

Scope and definitions

§ 1. This notice shall apply to animals subject to the animal testing law or the provisions of provisions adopted pursuant to Article 1 (1) of the Law. 7.

Paragraph 2. Furthermore, this notice shall apply to animals caught, bred or stored in order to be used or in order to use their organs or tissues to try.

Paragraph 3. However, the animal husbanding in the agricultural production sector shall not be covered by the notice until the animal is transferred to a supplier ' s holding shall be transferred to the place where the study is to be carried out, or, where the experiment is to be carried out in the crew, from it ; The time of the experiment.

§ 2. For the purposes of this notice :

1) Attempts shall be made : any invasive or non-invasive use of an animal for experimental purposes or other scientific purposes, with known or unknown result, or educational purposes which may cause the animal pain, suffering, anxious or permanent damage, To or stronger than the introduction of a needle, in accordance with good veterinary practice, it shall cause. This includes all actions intended for or likely to lead to the birth or development of an animal or development and conservation of new genetically modified animal origin in such a condition, but excluding the killing of animals for the purpose of : use of their organs or tissues.

2) Animals which are subject to the law on animal testing or of provisions adopted pursuant to Article 1 (1) of the law. 7, and animals that are caught, reared or disbarred in order to be used or in order to use their organs or tissues to try.

3) Project : A work programme which has a specific scientific purpose and involves one or more tests.

4) Premises where experimental animals are kept, including any facility, any building, group of buildings or other premises, including places that are not completely sealed or covered, farms, outdoor entries, equipment for breeding ; laboratory animals and similar delimited areas, mobile installations, laboratory testing premises and other experimental sites in which experimental animals are residing for longer periods.

5) housing, housing, stable and cultivated, etc., in which experimental animal facilities are located.

6) Experimental conditions : any defined area as accovaries, terrors, cages, entries, and similar areas specially designed for the testing of animals of the test species.

7) To be killed : any natural or legal person who, with or without any credit for the eye, rearing the animals referred to in Annex 1 for animals to be used for tests, or for the purpose of their tissues or organs to be used for scientific purposes ; cause or rearing other animals primarily for these purposes.

8) Vendor : Any natural or legal person other than a farmer who, with or without merit, provides animals for the use of animals for tests, or for the purpose of their tissues or organs to be used for : scientific purposes.

9) The animal test holder : Any natural or legal person who uses animals for tests with or without a profit.

10) Substitution : Replacement of conscious living higher animals with the ability to perceive external influences with material without any means of percepting effects.

11) Limitation : Reduction in the number of animals used to achieve a certain amount of knowledge with the necessary precision.

12) Finalness : any reduction in the number or level of test methods carried out on the animals to which it is still necessary to apply.

13) Invalidating suffering : Reduction of a person's normal physical or psychological ability to function.

14) The self-preservation colony : a colony in which animals are bred only in the colony or strains from other colonies and not be caught in the wild and where the animals are kept in a way which ensures that they are accustomed to humans.

Chapter 2

Use of certain animals for tests

§ 3. Human monkeys must not be used for testing.

§ 4. Non-human primates may not be used for testing.

Paragraph 2. However, the animal test subject may grant permission, cf. however, section 5, if the purpose of the study is covered by No 1. In the case of 1, 2, or 3, there is a scientific justification for the fact that the purpose of the experiment cannot be achieved through the use of any species other than non-human primates :

1) Prevention of disease, poor health or other abnormality and the effects of this in humans, animals and plants, including the production of medicinal products, substances and products, and testing of their quality, efficacy and safety, cf. however, paragraph 1 3.

2) Diagnostic and processing of disease, poor state of health or other abnormality and the effects thereof in humans, animals and plants, cf. however, paragraph 1 3.

3) Basic research and research for species conservation.

Paragraph 3. Authorisation pursuant to paragraph 1. 2, number 1-3, may only be communicated to which the study is carried out in order to avoid, prevent, diagnose or treat invalidity, or potentially life-threatening clinical conditions of human beings.

§ 5. Non-human primates listed in Annex A to Regulation No 2 ; 338/97 of 9. In December 1996 on the protection of wild fauna and flora by checking trade therein, and which do not fall within the scope of Article 7 (1), Paragraph 1 of this Regulation may not be used for tests.

Paragraph 2. However, the animal test subject may grant authorization if the purpose of the experiment is covered by paragraph 1. In the case of the use of species not listed in that Annex, 1, 2, 3 or 4, and there is a scientific justification for the purpose of the experiment not being complied with in the use of species other than non-human primates or other species.

1) Prevention of disease, poor health or other abnormality and the effects of this in humans, animals and plants, including the production of medicinal products, substances and products, and testing of their quality, efficacy and safety, cf. however, paragraph 1 3.

2) Diagnostic and processing of disease, poor state of health or other abnormality and the effects thereof in humans, animals and plants, cf. however, paragraph 1 3.

3) Research for species conservation.

4) Basic research.

Paragraph 3. Authorisation pursuant to paragraph 1. 2, no. 1 4, may only be communicated to which the study is carried out in order to avoid, prevent, diagnose or treat invalidity or potentially life-threatening clinical conditions of human life.

§ 6. Except for non-human primates, cf. in section 5, animals shall be listed in Annex A to Regulation No 2 ; 338/97 of 9. In December 1996 on the protection of wild fauna and flora by checking trade therein, and which do not fall within the scope of Article 7 (1), 1, in that Regulation, shall not be used for tests.

Paragraph 2. However, the animal test subject may grant authorization if the purpose of the experiment is covered by paragraph 1. In the case of a species not covered by paragraph 1, 1, 2 or 3 and there is a scientific justification for the purpose of the experiment. 1 :

1) Prevention of illness, poor health or other abnormality and the effects of this in humans, animals and plants, including the production of medicinal products, substances and products, and testing of their quality, efficiency and safety.

2) Diagnostic and treatment of disease, poor state of health, or other abnormality and the effects of this in humans, animals and plants.

3) Research for species conservation.

§ 7. Animals that are contained in nature shall not be used for tests.

Paragraph 2. However, the animal test subject may grant authorisation if there is a scientific justification for the fact that the purpose of the experiment cannot be carried out by the use of animals raised for tests.

§ 8. The intake of animals in the natural environment to be used for testing must be carried out in a spirit of the animal and the relics of wildlife ; the tab must be carried out only by persons approved by the Animal test.

Paragraph 2. Where it is found that an animal has suffered injury or has weakened state of health, a veterinarian or other competent person should be considered as soon as possible, and appropriate measures must be taken to limit the animal's animal ; suffering. If the animal has suffered serious damage, the animal shall be put to life immediately. For the killing, section 46 shall apply mutatis muth.

Paragraph 3. Transport containers and means of transport suitable for the species concerned shall be available at the disposal site in the event of a need to move animals for examination or treatment.

Paragraph 4. Appropriate measures shall be taken to ensure accreditation, quarantine, storage, care and care of animals trapped in the wild.

Paragraph 5. The study may dispense with the requirement that measures should be taken to limit the animal's suffering if there is a scientific justification for it. The animal test facility may, in particular, grant permission that an animal is not immediately killed if it has suffered serious damage.

Paragraph 6. In the event of a reproduction of animals in the case of tests, the general rule shall apply in section 1 of the animal testing law.

§ 9. Herreless and wild livestock may not be used for tests.

Paragraph 2. However, the animal test facility may grant authorisation if studies are essential to the health and welfare or serious threats to the environment or human or animal health, and there is a scientific justification for the purpose of the study. the purpose of the experiment may be achieved only by the use of stray or wild animal huspets.

§ 10. Animals of the species listed in Annex 1 to this notice shall be used only for tests if they are bred for experimental purposes.

Paragraph 2. Non-human primates as listed in Annex II to Directive 2010 /63/EC of the European Parliament and of the Council. In September 2010 on the protection of animals used for scientific purposes, only with effect from the dates specified in the Annex shall be used for tests if the descendants of non-human primates were bred in captivity or if they are : derives from self-sustains colonies.

Paragraph 3. However, the animal test subject may authorise the use of animals not reared for tests, if it is scientifically justified.

Paragraph 4. For animals originating from abroad, information must be provided that proof that the animals of the country of origin are reared for tests.

§ 11. Ceretics of non-human primates must draw up a strategy to increase the proportion of non-human primates, which are descended from non-human primates, which are inbred in captivity.

Paragraph 2. The strategy must, along with the application for breeders of non-human primates, must be submitted to the Animal test surveillance.

Chapter 3

Reuse of test animals

§ 12. In the case of a choice between animals that have been used for experiments and animals which have not been used for experiments, the used animals must only be recyced when :

1) the specific workload of the previous attempt was easily or moderate ;

2) the applicant has demonstrated that the general health and well-being of the animal is fully restored,

3) the new test is classified as easy or moderating or terminally incriminating ; and

4) taking into account what the animal has lived through in its lifetime is in accordance with a veterinary evaluation.

Paragraph 2. The animal test may be authorized in specific cases and after a veterinarian has examined the animal to allow an animal which has been used for experiments which caused considerable pain, anxiety or similar suffering to an animal which has been used for an animal which has been used in the course of such aggrieving pain, anxiety or similar suffering. Retry. Paragraph 1, no. 2-4 shall apply mutatis mutis.

Chapter 4

Journal Pre-Journal

§ 13. The test of animal testing must be carried out by the animal who tests it. The same applies to persons reared or supplying laboratory animals.

Paragraph 2. The journal must refer to the authorisation of the Animal test subject and contain information about :

1) the number and types of animals raised, acquired, supplied, shall be used in experiments, deferred or rehoused ;

2) the origin of the animals, including whether they are bred for experimental purposes ;

3) the date on which the animals are acquired, delivered, deferred or reproduced ;

4) the animals are acquired,

5) the name and address of the recipient of the animals ; and

6) the number and nature of animals that are dead or killed. For animals that are dead, the cause of death shall be noted if it is known.

Paragraph 3. In the case of the animal testing procedure, the file must in addition to the information provided for in paragraph 1. 2 include information on :

1) The type of seafarers and the technique of congreing.

2) Underanesthesia has been used and, where appropriate, its form.

3) The time for the animation of the animals and by the procedure used. If they have not taken place, the state and use of the animal shall be indicated after the experiment is indicated.

Paragraph 4. Where animals have been produced by cloning or re-modification, or have cloned or genetically modified animals been used for animal experiments, the records shall, in addition to the information provided for in paragraph 1, shall be required. 2 and 3 contain a separate indication of the nature and number of these animals.

§ 14. The journal shall be kept in a format approved by the Animal test subject. The animal test can, in particular, decide that the journal is carried out in an electronic journal format.

Paragraph 2. Journals must be carried out on the same day as the tests are carried out. However, where a routine uniform experiment is carried out in greater numbers, such experiments may be introduced by a group for one week at a time.

§ 15. Journals after section 13 must at all times be available to the animal test sight.

Paragraph 2. The records shall be kept for at least five years from the end of the calendar year in which the experimental animal is reared or delivered, or where the animal experiment has been carried out.

§ 16. Journals under Clause 13 shall be submitted each year to the Animal test. Journals shall be accompanied by statistical information on the experiments carried out and on cloning and remodification of test animals. The statistical information must indicate the extent to which each animal has been subjected. It shall also show the number of animals and species produced by cloning or re-modification, as well as how many cloned and genetically modified animals have been used for animal testing and for the collection of tissues.

§ 17. The study may lay down detailed guidelines for the design and the formation of journal and statistical information.

Chapter 5

Information, labelling and identification of dogs, cats and non-human primates ;

§ 18. Dogs, cats and non-human primates must, in addition to the requirement of section 13, have an individual record that follows the animal. The records shall be prepared at birth or, as soon as possible, thereafter.

Paragraph 2. The journal shall contain information about :

1) identity,

2) the food and food date when it is available ;

3) whether the animal is bred for experimental purposes, and

4) in the case of a non-human primate, whether this descendant of non-human primates has been bred in captivity.

Paragraph 3. The records shall, in addition to the information provided for in paragraph 2 include all relevant breeding, veterinary medical and social information relating to the animal and the tests to which the animal has been used.

Paragraph 4. Information after this provision shall be made available at all times for the experimental supervision of the Animal.

Paragraph 5. Information after this provision must be kept for at least three years after the death or relocation of the animal.

§ 19. Dogs, cats and non-human primates must be marked in a way that ensures that individual animals can always be identified. Labelling must be made no later than the wetback.

Paragraph 2. If there are dogs, cats or non-human primates, who are weaned and which are not labelled, tagging shall be carried out without undue delay.

Paragraph 3. Where a dog, cat or non-human priate before the transfer is transferred from a farmed, supplier or animal test permit holder, and for practical reasons, the animal must, until the animal is marked, shall be entitled to : keep records of the animal, including the entry of the parent animal.

Chapter 6

Phrase and resettlement of laboratory animals

20. Animals may be rehoused or put back in a habitat or production system appropriate to the species, if :

1) the state of the animal permits it,

2) there is no danger to public health, animal health or the environment ;

3) appropriate measures have been taken to ensure the well-being of the animal ;

4) a plan has been drawn up for the rehabilitation of the animals concerned, including being socialized, and

5) the veterinarian or other competent person who is assigned to the institution or the undertaking to advise the welfare and behaviour of the study animals has approved paragraphs 1 to 4.

Paragraph 2. For dogs, cats and non-human primates that are re-housed shall be valid in addition to the requirement laid down in paragraph 1. 1 that the journal after paragraph 18 (1) is that : 3, with veterinary medicinal and social information, accompany the animal.

Paragraph 3. Information referred to in paragraph 1 1 shall, before the animals in question be rehoused or returned in a habitat or production system appropriate to the species, shall be submitted to the Animal Experimental Commission.

Chapter 7

General provisions on care and disbarding

§ 21. In accordance with the rules laid down in this notice, the keepers, vendors and the animal test holder shall ensure that the experimental animals are treated responsibly and that pain, suffering, distress and permanent damage are minimised. Closer, vendors and animal testing holders must also ensure that all the deficiencies or the avoidance of pain, suffering, distress or permanent damage are resolved as soon as possible.

Paragraph 2. The one in paragraph 1. Paragraph 1 shall also apply to the natural or legal person who is responsible for the care and care of the experimental animals in accordance with Article 2 (2). Three, in the case of animal testing.

§ 22. The persons referred to in section 21 must at least take care that :

1) the laboratory animals are being housed, fed, watered, nurtured, nurtured and taken into account, taking into account the maintenance of their health and well-being,

2) physiological, behavioural and health requirements of study animals, including, for example, access to water and food, stockings, environmental enrichment, the possibility of physical expression and social contact, the possibility of use of employment materials and access to the best account is met as well as possible ;

3) the design of the selected environmental enrichment, the design of the experimental veterinary design and the nature, size and number of the animals and so on has sufficient freedom of movement and, where necessary, to be given additional space, in addition to those listed in Annex 2 ; minimum requirements ;

4) the physical conditions under which animals are reared, kept or used are inspected daily in accordance with updated, recognised practical and scientific experience ;

5) the staff working with the test animals have the necessary knowledge of the natural behaviour and biology of species, and that the care of the animals is reordering and with respect for the integrity of the animals, and

6) in the case of the planning of the work carried out at the experimental sites, there is a time for social contact between staff and laboratory animals, including frequent handling and training etc., where this meets the welfare of the animals and is in accordance with the study of the study ; purpose.

-23. The persons referred to in Section 21 shall, in any case, ensure that the minimum requirements set out in Annex 2 to this notice are complied with.

Chapter 8

The places of life

§ 24. The installation sites must be constructed, adjusted and maintained in such a way as to ensure that the physiological, behavioural and health requirements of each animal species are best safekeeping.

Paragraph 2. Businesses must have an active maintenance programme with a view to preventing and dismissing errors and defects in buildings or equipment.

§ 25. In so far as possible, the places of installation shall be subject to the protection of animals being protected from access to unauthorised persons and animals, including vermin, and other disruptions.

SECTION 26. The places of installation shall be so as to be so as to not escape the test.

§ 27. All the storage places must be equipped in a manner that limits any inconvenience to the test animals in the case of relocations to and from the place of installation.

Paragraph 2. It may be possible to isolate new entrants or recurrent laboratory animals until an assessment of the health condition of the experimental animal and any risk of infection has been minimized. However, such special measures may be omitted, for example when the newly added test animals cannot result in a risk to the other laboratory animals, including when the newly entranted test animals are all having a proven health status which is likely to prove that they are not constituted ; a health hazard for the other laboratory animals or persons.

Paragraph 3. If, in addition, the presence of certain test animals may constitute a health hazard for experimental animals or persons, the establishment of the laboratory must be able to isolate the animals concerned until such time as the risk is restricted in a safe way ; scope.

§ 28. The cleanup places must be possible to separate sick and injured experimental animals or experimental animals that are otherwise weakened by the other test animals.

§ 29. All options must be equipped and designed so that the collection of samples, simple diagnostic tests, killing and simple post-mortem examinations can be carried out on the ground. In addition, the storage places must be so designed that killing, post-mortem examinations and other tests or observations which are not desirable to carry out in the enclosure room may be carried out in separate premises separated from : the storage rooms.

Paragraph 2. Where there is a need for surgical experiments under aseptic conditions, one or more appropriate equipped space and facilities for re-convalescence shall be provided after operations.

-$30. The storage facilities must accommodate appropriate remediation, disinfecting and cleaning, etc., of used equipment. The cleaning process must be arranged in such a way as to keep the flow of clean and dirty equipment separated to prevent contamination of the very cleansed equipment.

Paragraph 2. Clean cages, tools and other equipment must be able to be stored separately from other equipment.

§ 31. Containers for feed and litter must be adapted, designed, used and maintained in such a way as to ensure that the storage of feed and sock can be carried out in an appropriate manner and under clean and hygienic conditions, and so that no deterioration is made. It must be ensured as far as possible that pests or insects do not have access to the feed and the stockings.

Paragraph 2. Other materials which may be contaminated or present a hazard to animals or personnel must be stored separately.

§ 32. Dead animals and animal waste products must be treated, stored, transported and so on in such a way as to prevent the spread of infectious or other harmful substances.

§ 33. A alert system must be set up as well as a contingency plan for rapid intervention on fire or other sudden emergencies and failure of the technical installations if a failure of the installations could result in a risk ; in the case of health and welfare of the laboratory animals.

Paragraph 2. The alarm system must be adjusted in such a way as to allow a possible alarm signal to cause the animals at least, unless it goes beyond the alarms of the alarms of human beings.

Chapter 9

The stables rooms

§ 34. The accommodation premises must be designed, constructed and maintained in such a way that the physiological, behavioural and health requirements of each animal species are best taken into account.

$35. The accommodation rooms must be designed and designed to maintain good hygienic standards. Cleaning, washing and disinfection of remotenable plant units which have been in contact with laboratory animals shall be available. Carve, walls, ceilings and equipment, etc. must be of suitable materials, so that the cleaning and disinfection can easily be carried out.

Paragraph 2. It must be ensured that the materials used do not pose a risk to the welfare of the animal animals, including their health.

§ 36. The premises must be fitted and equipped in a manner that takes into account that the experimental animals must be capable of being handled and seen to be insofar, including the possibility of adequate illumination for the inspection of all laboratory animals, are kept in a reassuring manner.

Paragraph 2. Where albino animals are held, the lighting must be organized in such a way as to take appropriate account of their illuminating sensitivity.

§ 37. If several experimental animals are placed in the same room, they must be constructed and placed in such a way that the test animals are not exposed to stress or have the possibility of harpossing each other.

Paragraph 2. Predators and prey animals must not be placed in the same room or they can see, smell or hear each other. Other species which are incompatible or any animal with different requirements for environmental conditions shall not be placed in the same premises.

§ 38. The isolation, heating and ventilation of the housing premises must ensure that there is a sufficient air change and that the dust content, relative humidity and concentrations of gases are kept at a level which is not harmful or generative to : the test animals.

Paragraph 2. Ventilation systems shall not result in unnecessary traits or noise.

Paragraph 3. Smoking is not permitted in the housing premises in which laboratory animals are located.

§ 39. The temperature and humidity in a storage room must be adapted to the needs of the individual experimental animals, including, for example, newborns, new-born, injured, injured, sick or pelt and the special needs of the animals ; the temperature and humidity must be measured and are recorded daily.

§ 40. In the storage rooms, direct daylight or artificial lighting must be accessible to allow the test animals to maintain normal activity and behaviour. The conditions of light must be regulated so that they do not result in discomfort or damage to the experimental animals and so that the test animals are given the light and dark times of the kind for the species the length necessary.

§ 41. The accommodation must be adapted in a manner that ensures that the test animals are best protected from unnecessary noise.

Chapter 10

Forsisanitation plant

§ 42. The veterinary services must be designed, corrected and maintained in such a way that the physiological, behavioural and health requirements of each animal species are best taken into account and that adequate cleaning, including the mugging and the need to be used, must be adequately cleaned up ; disinfecting may be carried out, except where disposals are used, and that the experimental animals may be seen without any unnecessary disturbence.

§ 43. In accordance with Annex 2 to this notice, the veterinary carers must be such as to have the possibility of the experimental animals as far as possible to develop their natural behaviors.

§ 44. In accordance with Annex 2 to this notice, the veterinary carers shall be in conformity with environmental enrichment, which, in relation to the species-specific and individual needs of each animal, contributes to the development of natural behaviour of the experimental animal, This includes the possibility and promotion of exercise, ourails and the unfolding of other natural activities.

Paragraph 2. The installation must, as appropriate for the species, be a fixed, comfortable rest area to all animals. All bedrooms shall be kept clean and dry.

Paragraph 3. The environmental enrichment must be constantly adapted to the needs of the animals.

§ 45. Animals must be disbarred in accordance with the harmonious groups, together with species, except in the case of species which are from the natural side.

Paragraph 2. Experienced animals may, however, be separated from species whose group is likely to cause the animals to be harmful to each other, including group litigation of male genital animals of certain species.

Paragraph 3. If laboratory animals are individually listed, cf. paragraph In the shortest possible time period, or in accordance with an exception, the single veterinary charge shall be carried out during the shortest possible time and the animals must be able to see, hear, smell and have physical contact with the species. In addition to this, it must be ensured through increased environmental enrichment that the experimental animals must be at least possible by being incarnation alone.

Paragraph 4. Sick and injured experimental animals or experimental animals which are otherwise weakened must be separated from the other laboratory animals whose group installation will cause danger to the welfare of the animals. However, it must be ensured as far as possible that the study animals can see, hear, smell and have physical contact with the species.

Chapter 11

Transport and killing of laboratory animals

§ 46. Levitation of test animals shall be carried out in a place of life of a competent person who is educated herein, cf. § 58, paragraph. 5. However, in the case of field studies, an animal may be killed by a competent person outside of a place of life.

Paragraph 2. Levitation of test animals must be carried out in conditions ensuring that the experimental animals suffer at least the suffering of pain, suffering and anxiety.

Paragraph 3. For the choice of method of killing, the individual and species specific characteristics of the animal must be taken into account in accordance with the procedure for taking up the method of life. however, paragraph 1 4. Compensation must also be carried out in accordance with updated, infamous practical and scientific experience.

Paragraph 4. Animals referred to in Annex 3 shall be killed in accordance with an appropriate method as specified in the Annex.

Paragraph 5. The animal testing may be dispensers from the requirement referred to in paragraph 1. 4 in the following cases :

1) in order to permit the use of another method provided that the method on the basis of scientific evidence is considered to be at least as humane, or

2) where there is a scientific justification for the fact that the purpose of the experiment cannot be achieved by applying a method of killing which is indicated in Annex 3.

Paragraph 6. The animal test can provide a way to make an opening of how to make the killing happen.

Paragraph 7. If, in an emergency situation, an animal of animal welfare, public health, animal health or the environment is necessary, it is necessary to put an animal on animal welfare, public health, animal health or the environment. 2 use.

§ 47. Presible conditions must be transported under appropriate conditions.

Paragraph 2. In addition, in the case of transport of animals, the rules in the notice on the protection of animals during transport and the Council Regulation (EC) No (EC) No, 1/2005 of 22. In December 2004 on the protection of animals during transport and related activities and the additional rules laid down in the notice of training in the transport of animals.

Chapter 12

The care of the seafarers, the care and the others.

§ 48. The warmings shall be used as far as possible to the close contact with human beings so that each test animal through frequent contacts is kept confidential to the presence and activity of humans. If necessary, the animals must be trained in daily routines.

Paragraph 2. The group of animals to be discharged must, if necessary, be socialized into harmonious groups.

§ 49. In the case of the entrance of an experimental animal to a place of housing or the place where testing is to be carried out, the welfare and health of test animals must be carried out in the light of the welfare and health of the animals in question ; appraisal is necessary in order to acclamatise the test animal to the new conditions.

$50. The experimental conditions must be trained to the test situation as necessary, so that stress and other suffering in connection with the study is avoided as much as possible.

Paragraph 2. However, training of the test animals may be omitted if, in itself, the exercise in itself will result in stress or other suffering which corresponds to or exceeds the test to be carried out by the experimental animal.

§ 51. Resume animals which are in a shorter or longer time outdoor must, in accordance with their needs, be protected from excess as a result of the weather and temperature conditions. The animals ' ferries must not be limited to outdoor areas under climatic conditions, which may be a mental burden on animals.

Paragraph 2. In the case of complete or partial outdoor, the temperature of the temperature shall be monitored and recorded.

§ 52. Labelling of experimental animals for identification and so forth must be so painfully as painless as possible and may only be carried out by a veterinarian or a person who is trained and trained to carry out the marking. If the label will cause strong pain or discomfort to the test animal, it shall be used for anesthesia or anesthesia.

Paragraph 2. The labelling shall not result in the durable beds for the test animal.

Chapter 13

Veterinary advice, supervision, control, etc.

§ 53. Ceretics, vendors and the animal test permit holders must be associated with a veterinarian specialising in laboratory animals or other competent person to advise on the welfare and treatment of experimental animals and appropriate measures ; measures to ensure such appropriate and adequate advice.

Paragraph 2. The animal testing may provide for instructions to be drawn up on internal and external veterinary checks carried out in the institution or the establishment, and the instruction to be submitted to the supervision of approval.

Paragraph 3. There must be a record of how external, advisory assistance has been provided. Journal recordings shall be kept for five years from the end of the calendar year.

Paragraph 4. In the institution or by the establishment, there must be a sufficient number of trained staff to ensure compliance with the rules on the protection of the animals.

§ 54. Rivers, vendors and the animal test holder and persons who, under the authorisation of an authorisation holder, are engaged in experiments and persons employed to care and care for the animals, must take measures to : to ensure that all the deficiencies or suffering identified are remediable as soon as possible.

Paragraph 2. There must be regular checks on the health and welfare of the animals. The controls must be led by a veterinarian or by persons who have been instructed and supervised.

Paragraph 3. All animal-tested laboratory animals must be looked at as necessary and at least once a daily, so that damage and disease may be detected quickly and the test animals may be considered appropriate.

§ 55. In the performance of its supervisory activities, the animal testing must ensure that the requirements of experimental animals may be met in the experimental animals used, in particular in respect of the size and number of the laboratory animals and the extent of the environmental enrichment ; and the spatiarity of the experimental animal sanity.

Chapter 14

Qualification requirements for persons dealing with experimental animals

§ 56. Permission for animal testing may only be granted to applicants who have passed a relevant candidate training in the area of health or science, together with one or more courses that are relevant to the test activity, including : the animal species covered by the application.

Paragraph 2. The people in paragraph 3. 1 that are aimed at ensuring that the applicant can use experimental animals after a high ethical and scientific standard, and shall include :

1) legislation, as well as ethical and animal welfare-related aspects relating to the team and use of test animals,

2) knowledge of experimental animals, including biology and health, and teams, use, handling and breeding of test animals,

3) general exploration, including pain and stress-related behaviors and anaesthesia and pain treatment, and

4) theoretical and practical matters of the design, planning, implementation and evaluation of animal tests.

Paragraph 3. In the case of persons responsible for the design of animal tests, as well as persons covered by Article 2 (2) of the Animal Experienlist Act. 2, no. Paragraph 1 shall apply. 1 and 2 similar uses.

§ 57. The test permit holder or the person responsible for the execution of the tests may not be carried out by other tests if they are not ;

1) have completed the training of animal zookeeper specialists in laboratory animals, cf. the notice of the training of the professional community, animals, plants and nature, and may prove to have the necessary knowledge of the species or animal species to which the experiments are to be used,

2) is in the case of the training of animal zookeeper specialists and has completed step 1 and the first 6 months of step 2, cf. the notice of the training of the professional community, animals, plants and nature, and may prove to have the necessary knowledge of the species or animal species to which the experiments are to be used,

3) have carried out the previous agricultural training with special animal assistant and specialties of study specimens and may prove to have the necessary knowledge of the animal or animal species for which the experiments are to be carried out ; or

4) have other relevant training and have undergone a theoretical and practical course in the performance of animal tests, including those relating to the animal or species for which the experiments are to be carried out.

Paragraph 2. The one in paragraph 1. 1, no. 4, mentioned course must include :

1) legislation, as well as ethical and animal welfare-related aspects relating to the team and use of test animals,

2) knowledge of experimental animals, including biology and the health and safety of the animals, handling and breeding of laboratory animals ; and

3) general exploratory testing, including pain and stress-related behaviors and anaesthesia and pain treatment.

Paragraph 3. Knowledge of the animal species which the tests are related to, cf. paragraph 1, no. 1-4, can be achieved through training in the institution or the establishment. Persons covered by paragraph 1. 1, no. 1-4, work under supervision until they have shown that they have the necessary skills.

§ 58. The holder of the authorization or the person responsible for the responsibilities of the personnel concerned with the care of the experimental animals shall ensure that persons who are employed to care and care for laboratory animals are employed by the hire ;

1) have completed the training of animal zookeeper, cf. the notice of the training of the professional community, animals, plants and nature, and may prove to have adequate knowledge of the species or animal species to which the person concerned must take care and care ;

2) have completed agricultural training of specialised farmer, livestock, cf. the notice of the training of the professional community, animals, plants and nature, and may prove to have adequate knowledge of the species or animal species to which the person concerned must take care and care ;

3) completed the training of veterinary nurse, cf. the notice of the training of the professional community in the area of animals, plants and nature, and can document the necessary knowledge of the species or animal species to which the person in question must take care or care ; or

4) otherwise acquired qualifications, including abroad, which, in the case of care and care of animals, is equivalent to having completed one of the products referred to in paragraph 1. 1-3 training courses referred to, and they may be able to document the necessary knowledge of the species or animal species to which the person concerned must take care and care.

Paragraph 2. Knowledge of the species or animal species to which the person concerned must fit and care, cf. paragraph 1, no. 1-4, can be achieved through training in the institution or the establishment. Persons covered by paragraph 1. 1, no. 1-4, work under supervision until they have shown that they have the necessary skills.

Paragraph 3. If the Animal Experimental Commission has authorized the responsibility for the part of the animal test activity, which is to be taken into account and for the accommodation and the care of the animals and the care of the animals, the institution or the undertaking shall be borne by the institution or the undertaking. Section 2 (2). The provisions of paragraph 3 of this Article shall be the responsibility of the provisions of paragraph 3. 1, responsibility shall be responsible for this institution or undertaking.

Paragraph 4. The requirements of paragraph 1. 1, no. 1 4, does not include persons who are in training in order to obtain an independent responsibility later in order to take care of and care for experimental animals.

Paragraph 5. For persons carrying out experimental animals, the requirements laid down in paragraph 1 shall be laid down. 1-2, corresponding use.

$59. After obtained the opinion of the Animal Control Committee, the FDA shall approve the examination plans for the tests referred to in section 56 (3). Rule 57 (1) and Section 57 (3). 1, no. Four, mentioned courses.

§ 60. The study may dispensers from the requirement in section 56 (s). 1, where the applicant has previously been authorised for or otherwise engaged in animal experiments in this country or abroad, and has qualifications which correspond to the implementation of the provisions of section 56 (3). 1, the course for the species or animal species to which the application relates.

Paragraph 2. Paragraph 1 shall apply mutatis muctis to persons covered by section 56 (3). 3.

§ 61. The animal study may dispense with the requirement in section 57 (3). 1 if the persons referred to in the provision have been engaged in animal experiments in this country or abroad and have qualifications which correspond to the implementation of the section 57 (3). 1, no. 4, the course for the species or animal species for which the tests are to be used. § 57, paragraph. 3, shall apply mutatis mutis.

Chapter 15

Application for authorisation for animal testing

§ 62. An application for authorization to animal experiments shall be submitted to the animal test subject.

Paragraph 2. The application shall include :

1) a description of the project,

2) a non-technical description of the project, cf. § 63,

3) a description of the tests provided for in the project description, including the use of the animals,

4) information on origin, estimated number, species, and life stages of the animals expected to be used,

5) the application of methods of compensation, restriction and refinement in connection with the tests,

6) the planned use of anesthesia, analgetics and other forms of pain relief,

7) limiting, avoidance and relief of any kind of animal suffering from birth to death when it is appropriate,

8) the use of human endpoints,

9) the study strategy and statistical planning with a view to reducing the number of animals, pain, suffering, anxiety and environmental impact, where appropriate,

10) the reuse of animals and the cumulative effect of the animals on animals,

11) the proposed classification of the study after the incapacity of the load, cf. Annex 4,

12) avoidance of injustifiable reoccurrence of tests, where appropriate,

13) information relating to the conditions under which the animals are placed, kept and nurtured ;

14) killing methods ; and

15) skills for those involved in the project.

§ 63. The non-technical description of the project, cf. § 62 (2) 2, no. 2, shall include :

1) a description of the purpose of the project,

2) a statement of the fact that similar knowledge is not wholly or partially achieved without the use of live animals, and cannot be achieved by means of less incriminating investigations or by the use of fewer animals,

3) a statement of the fact that the trial is of significant use, and

4) a description of all the interventions, including the number of animals and the animals to be used, and a detailed description of the load to which the animals concerned are exposed.

§ 64. The animal test shall publish in anonymized form, as a minimum, section C of the application for authorization to carry out animal tests. The schema includes a description of the attempt, including the non-technical description of the project.

Chapter 16

Permission for breeding and delivery of test animals and animal tests

§ 65. The animal test must decide within 40 working days of receipt of the application and any necessary information.

Paragraph 2. The period referred to in paragraph 1 1 may be extended once more than 15 working days, if due to the nature of the case, it is not possible to make a decision before the end of the deadline. If the deadline is extended, the applicant shall be given prior to the expiry of the time limit, and the date on which a decision is taken at the latest. The notification shall show what the justification is for the extension and for the period laid down.

Paragraph 3. The test must be sent as soon as possible upon receipt of an application to the applicant on the receipt of the application. The confirmation must contain information about the time limit referred to in paragraph 1. 1 and on the availability of the period referred to in paragraph 1. 2.

Paragraph 4. Where an application is incomplete or incorrect, the animal test subject shall inform the applicant as soon as possible with a request to receive the missing information. The test must at the same time inform the applicant of the time limit referred to in paragraph 1. 1.

Paragraph 5. Exceeding the time limit laid down in paragraph 1. 1 or the prolonged period after paragraph 1. 2 shall not impanse the applicant ' s authorization of notification.

§ 66. The person who has received a permit relating to activities carried out within the framework of a service shall provide the person or persons for which the undertaking is carried out, contact information relating to the Animal test subject. Such information shall be made available or made available to the recipient of the service prior to an agreement on the provision of the service.

Paragraph 2. The information provided in paragraph 1 1 is given or made available in the manner in which the undertaking finds the most suitable. This information may be made available via electronic means via an address indicated by the person concerned or knows that the information is available where the company is located.

Chapter 17

Subsequent Evaluation

§ 67. The animal test permit holder shall, where a subsequent trial evaluation is made by the Council for Animal experiments, shall communicate the following information to the Animal test subject :

1) the purpose of the project was achieved,

2) the nature and number of animals used,

3) the harmful effects of the animals used,

4) the classification of the testing capacity of the tests, and

5) the principles of compensation, restriction and refinement.

Paragraph 2. The information must be submitted within 30 days of the expiry of the authorization for animal experiments.

Paragraph 3. Attempts have been classed as significantly incriminating and tests where non-human primates are used shall then evaluate.

Chapter 18

Special provisions for breeders and other vendors of laboratory animals

§ 68. Animals and delivery of laboratory animals may be subject to authorization from the Animal Experimental Authority.

Paragraph 2. Authorisation may only be granted to the person who has the necessary facilities for the purpose and whose operation is operated in such a way as to ensure safe control of the animals, including their origin.

Paragraph 3. Authorisation may be limited in respect of the nature of the species and may be laid down in the case of the authorisation, including breeding, storage, feeding, care, health inspection and inspection.

Paragraph 4. If the holder of the authorization is a legal person, it shall designate one or more persons responsible for

1) supervision of the welfare and care of the animals ;

2) training and training of staff engaged in the care of experimental animals.

Paragraph 5. The following information shall be included in a permit for the breeding or supply of laboratory animals :

1) Permit holder name.

2) In which institution or business breed may be carried out or the termination of laboratory animals, it must be carried out in the case of delivery.

3) Name of the person responsible or the competent authority of the staff engaged in the care of the experimental animals.

4) Name on the person or persons responsible for the care of the animals and the welfare of the animals.

5) Name of the veterinarian or other competent person who is assigned to the institution or the undertaking to advise the welfare and behaviour of the study animals.

Chapter 19

Animal welfare body

§ 69. Any animal test holder, rearing or supplier must set up an animal welfare body or be associated with an animal welfare body of the institution or establishment where the studies or rearing operations must be carried out or where the dislocation of : experimental animals must be carried out in the case of delivery.

Paragraph 2. The animal welfare body shall consist of at least the persons responsible for the care and supervision of the animals and the welfare of the animals and of the animal test holder of a member with a relevant scientific background.

Paragraph 3. The animal welfare body shall receive information from the veterinarian or other competent person who is associated with the institution or the undertaking to advise on the welfare and behaviour of the study animals.

§ 70. The animal welfare organ must

1) advise on questions relating to the welfare of the animals in relation to acquisition, housing, accommodation and use ;

2) advise on the application of the principles of compensation, restriction and refinement,

3) inform the technical and scientific developments in the field of compensation, restriction and refinement,

4) establish and update internal operational procedures for monitoring, reporting and follow-up on the welfare of the animals that are housed or used in the institution or establishment ;

5) monitor the development and performance of projects, taking into account the impact on the animals used and the identification and advice of factors which may be further entitled to compensation, limitation and refinement, and

6) to advise on plans on the relocation of animals to be rehoused or put back in a habitat or production system appropriate to the species, and shall be rehabilitated, including socialized.

§ 71. The animal welfare body shall keep records of the tasks performed in accordance with section 70 (s). 1-6.

Paragraph 2. Journals in accordance with paragraph 1. 1 shall be kept for at least three years and shall be made available to the Animal test subject.

Chapter 20

Final provisions

§ 72. The test carried out by the Animal test facility shall provide details of the tests on the experimental procedures.

Paragraph 2. Closer and vendors shall provide details of their reared and acquisition and disposal of animals in accordance with the request of the Animal experimental service.

Paragraph 3. Moreover, in addition to the information referred to in paragraph 1, the killing, vendors and the animal test permit holders must also be provided. 2 after the request of the Animal experimental service, details of the conditions covered by the notice shall be provided in accordance with the request.

§ 73. In the event of an emergency, a situation which, in the interests of the welfare of the animals, necessitates a derogation from the rules on care and storage in accordance with the conditions laid down in the case of the animals. Whereas the rules set out in Annex 2 of this notice shall be submitted within one week of the conduct of the tests on the Animal test subject.

Paragraph 2. Any deviation of conditions in a permit or by the rules of this notice and measures taken to remedy this must be recorded.

§ 74. The animal study may, for the sake of scientific consideration or animal welfare or animal health, and, after a specific assessment, dispensers from the requirements in section 21 to 45 and § § 48 -51, cf. however, paragraph 1 2.

Paragraph 2. It shall be subject to a derogation in accordance with paragraph 1. 1, ensure that :

1) the potential of the animals in order to satisfy their physiological and behavioural needs is limited to at least possible ;

2) the physical conditions under which animals are reared, kept or used are checked daily, and

3) all deficiencies or avoidance of pain, suffering, distress or permanent remedies shall be addressed as quickly as possible.

§ 75. For applications for the authorisation of products, products and so on, where data derived from animal experiments, is included as evidence, data from another Member State which originates from tests recognised in EU law, except where further attempts are made, are required in order to protect public health, public safety or the environment.

Chapter 21

Punishment, plining and revocation of authorisations

SECTION 76. The animal test subject may provide an injunction on the subject of sections 13, § § § § -19, § § 21-45, § § 48-52, § 54, § § § 57-58 and § 69.

§ 77. The animal test subject may withdraw a permit issued under this notice, provided that a serious or repeated violation of the rules in the notice is violated, by the conditions in question, or the equivalent of that has occurred ; disregarding an injunction.

Paragraph 2. The animal test must ensure that the withdrawal of the revocation does not impair the welfare of experimental animals.

§ 78. Unless higher penalties have been inflished on any other law, the penalty shall be penalised by the penalty which :

1) is in violation of section 3, section 4 (4). Paragraph 5, section 5. Paragraph 1, section 6, paragraph 6. Paragraph 7, paragraph 7. Paragraph 1, section 8, paragraph 8. 1-4. 9, paragraph. Paragraph 1, section 10, paragraph 10. Paragraph 1-2 and paragraph 1. 4, section 12, paragraph 1. 1, sections 13-16, § § 18-19, § § 21-45, § 46, paragraph 6. 1-4, section 47, paragraph .1, section 48-52, section 53, paragraph 1. Paragraph 1 and paragraph. 3-4, section 54, section 66, section 68, paragraph 6. 1, section 72, paragraph. 2, section 73, or

2) overrides an injunction in accordance with section 46 (3). 6, section 53, paragraph. 2, or section 76.

Paragraph 2. Companies can be imposed on companies, etc. (legal persons) punishable by the rules of the penal code 5. Chapter.

Chapter 22

Entry into force, transitional provisions, etc.

§ 79. The announcement shall enter into force on 1. February, 2013.

Paragraph 2. The following notices shall be deleted :

1) Publication no. 332 of 18. May 1990 on the acquisition of animals for tests.

2) Publication no. 1016 of 12. In December 2001 on the qualification requirements for persons concerned with experimental animals.

3) Publication no. 1273 of 12. In December 2005 on the lead on animal experimentation and to cloning and re-modification of vertebrates.

4) Publication no. 1320 of 17. In December 2009 on authorisation schemes according to the Animal Experimental Act.

5) Publication no. 997 of 16. August 2010 on the care and storage of experimental animals and the use of endangered and feral live animals for tests, etc.

$80. In the case of the cleanup places, housing and experimental animals that have been taken into service before 1. In September 2010, Annex 2 is found to be furtive. 2.6.2.3., 2.7.2.4., 2.8.2.2., 2.9.2.2., 2.9.2.5., 2. pkton, furtive. 2.10.2.2. and 2.10.2.5., 2. pkton, however, first of use from 1. September, 2014.

Paragraph 2. Similarly, Annex 2 shall be considered to be furtive. 2.6.4.1., 2.6.4.3., Table 1-4, point. 2.7.4.1., 2. -6. section, point. 2.7.4.3., 2.8.4.1., 2.8.4.6., 1. section, and furtive. 2.10.4.1., first apply to the first subparagraph of paragraph 1. 1 mentioned experimental animal health systems from 1. September, 2014, in so far as compliance with the provisions will predate significant changes in the direction and equipment of exploratory facilities. Before this date, these provisions shall be fulfilled as far as possible, taking into account the recovery of the facilities concerned.

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Appendix 1

Animals covered by section 10 (4). 1

1. Mouse (Mus musculus)

2. Rat (Rattus norvegicus)

3. Margs (Cavia porcellus)

4. Carrothhamster (Syrian hamster) (meetcricetus auratus)

5. Chinese hamster (Cricetulus griseus)

6. Desert rat (Meriones unguiculatus)

7. Kanin (Oryctolagus cuniculus)

8. Canis familiaris (Dog)

9. Cat (Felis catus)

10. All species of non-human primates

11. Seeds (laevis, tropicalis), Rana (temporaria, pipiens))

12. Zebrafish (Danio rerio)

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Appendix 2

Passing and the stalling of laboratory animals

2.1. Rodents

Mouse (Mus musculus)

Rat (Rattus norvegicus)

Margs (Cavia porcellus)

Hamster (Mesocricetus sp.)

Desert rat (Merione sp.) m.f.

2.1.1. Introduction

This Annex lays down a list of requirements for the installation of rodents used or used for tests.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the rodent is best taken on the basis of the specific conditions.

2.1.2. Climatic conditions, etc.

2.1.2.1. Ventilation

It shall be provided for adequate ventilation in the cleanup rooms and in the experimental livestock areas so that the needs of the unstabled are met. The ventilation system must be able to provide adequate fresh air of suitable quality, remove excess heat and humidity, and keep the quantity and spread of smelling, toxic gases, dust and infectious substances of any kind down.

Unless special circumstances apply, or natural ventilation by mechanical ventilation, air shall be carried out 15 to 20 times per. hour in the enclosure room. However, at low levels of stocking, the air shift may be reduced to 8 to 10 times per year. Hour.

It must be ensured that there is no harmful recirculation of untreated air.

The ventilation system must be installed in such a way as to avoid harmful features and noise nuisance.

Smoking is not permitted in rooms where the rodent rodents are in place.

2.1.2.2. Temperature

Rodents must be kept within a temperature range between 20 and 24 ° C. Among the groups of rodents in cages, the temperature must be measured by the stocking in the cage, unless it is considered inflambable to assume that the room temperature in the cages does not differ significantly from the temperature in the lime room.

The temperature in isolation systems and temperatures obtained for naked (hairless) must be carefully monitored.

2.1.2.3. Humidity

The relative humidity in plants for rodents must be kept at 55%. ± 10%. However, desert rats shall be disbarred by a relative humidity of 45%. ± 10%.

2.1.2.4. Lighting

The intensity of light inside the cage must be low ; in animal shelves, the upper row must be cut back so that the risk of nethondegeneration should be reduced.

Rodents must have regular light-and dark times of the kind for the species necessary length. However, if the observance of rodents in their active phase requires it may, in the dark, light illumination of red light at wavelengths that cannot be perceived by the gnawing.

2.1.2.5. Noise

Incoming noise, including ultrasound, must be kept at as low a level as possible.

High and sudden noises must be avoided as far as possible in the cleanup rooms.

It must be ensured that the rodent is shielded from ultrasonic noise (> 20 kHz), including the noise generated by ordinary laboratories, dripping cocks, computer monitors, wheels on rollertables, etc.

In case of an abnormal behaviour or an abnormal breeding pattern, due to ultrasonic noise, the acoustic environment must be monitored over a wide spectrum spectrum until the source of the noise has been identified, or the cause of the abnormal behaviour or the abnormal breeding pattern of other means has been determined.

2.1.2.6. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Heat, ventilation, cooling and bemoiling plants and similar installations which are significant for the welfare of the animals must be provided with monitoring devices and alarms to ensure that any possible errors can quickly be registered and remedied immediately.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care must be taken to ensure that the operation of the alarm systems disrupts the animals as little as possible.

2.1.3. Health

An action plan shall be provided by institutions or undertakings to ensure that an appropriate state of health is maintained, which protects the welfare of the species and meets the scientific requirements ; this action plan must include : microbiological monitoring programme and plans for the handling of major health problems, as well as the definition of health parameters and procedures for the reception of new rodents.

The person responsible for the test animals shall ensure a regular monitoring of the rodent and monitoring of their accommodation and care. The supervision must be carried out by a veterinarian or a corresponding competent person.

Supervision of the rodent must be carried out at least once a day by a person who is trained as a minimum in accordance with section 3 of the notice on the eligibility of persons dealing with experimental animals to ensure that all disease or injured persons are concerned. animals are identified and appropriate measures are taken to remedy problems.

Health monitoring must be carried out regularly.

hygienic procedures must be laid down to ensure that the potential risk of infection for rodent and staff to deal with the gnawing means shall be reduced as much as possible.

2.1.4. Astining, etc.

2.1.4.1. Restining

The Gnaverne must be placed in groups for as long as the groups can maintain stable and harmonious conditions.

However, the Gnaverne may be separated from species which will cause groupings to harm each other, as may be the case by group housing of adult male mice, grown-ups hamsters or desert rats, as these animals usually are, as they usually are ; exhibit strong aggressive behaviour towards species of species.

In addition, the Gnaverne may be placed separately if there is a likelihood of any adverse effects or damage to group housing. The resolution of established stable and harmonious groups must be avoided as far as possible, as this can cause stress.

2.1.4.2. Environmental enrichment

The burners and their environmental enrichment must make it possible for the rodent to display normal behaviour and to set the same species in a position to properly mitigate aggressive behaviour.

The stockings, the nesting and the shelter (safe houses) are very important environmental enrichment for rodents and must be available unless it is veterinary or welfare grounds that it is not valid.

The material must be such that the cranes can process it and build a nest. If sufficient evidence is not given enough to allow the animals to build a complete overdeck, additional account shall be made available to the animals. The stockings must be able to absorb the urine.

There must always be rats, mice, hamsters, and deserts of the nesting. In the same way, nesting boxes or other guinea pigs, hamsters and rats must always be given.

Margs must always have materials that can be processed, such as hay to chew and to use for cover.

Unless special circumstances apply, rodents must be allocated to chew and gnawing sticks.

If it is necessary to avoid struggles between group-barred rodents, partial barriers must be drawn up in the cages.

In order to increase the complexity of the surrounding area, there must be some form of environmental enrichment in the cage, such as pipes, boxes and climbing tribes.

Desert rats must be given the opportunity to build and / or use times of sufficient size. Desert rats must have a thick layer of stocking and nesting or replacement for a (subterranean) corridor that must be at least 20 cm long.

In the case of guinea pigs, it must be ensured that there is the possibility that all guinea pigs can seek cover at the same time.

In those cases where the sirs and surveillance of the gnawing cannot be carried out without causing the disturbings to be disturbed so much that there is a risk of the loss of well-being, partial transparent or toned cages and crow walls must be used.

The requirements of the quality and size of the space, environmental enrichment, etc. in this Annex also apply to isolation systems such as individual-ventilated cages (IVC = FFated cages).

2.1.4.3. Dimensions

The following and subsequent tables with minimum requirements for the installation of rodents means the vertical distance between the floor or the bottom of the floor and the upper horizontal part of the lid or the cage, and this height must be more than 50%. of the bundaarea of the agency.

The bundareal area is understood in all tables freely available in the bundaway, which the rodent can move-that is, that is : where permanent fixtures such as feed bowls and similar do not prevent the use of the enclosure area-and when calculating the minimum area, any land on shelves, crates and so on shall not be included.

In the case of experiments, the potential growth of the gnawing must be taken into account in order to ensure adequate space according to these tables at all stages of the tests. It must also be included in the planning that young active rodents are required to take more place than adults.

Table 1. Minimum requirements for the storage of mice

*Fraused mouse can be kept at one of these higher stocking degrees in the short period after resettlement until such time as the animals are placed in greater experimental animal health systems with sufficient environmental enrichment. Liability shall not result in the loss of welfare, such as increased aggregate, disease or mortality level, other behavioural abnormality, weight loss or other physiological or behavioural stress reactions, including stereotypical pees.

Table 2. Minimum requirements for the housing of rats

However, the height of the rat cage shall be at least sufficient to ensure that the rats in the cage in question can rise up on their hind legs.

** In the case of studies that span the life span of the animal (' lifetime studies `), the cages must be taken to the cages of the animals being housed in social terms. If the stocking density at the end of such tests, it shall mean that the allocated space per year shall be granted by the Member animals at the end of the experiment shall be attached to the maintenance of stable social structures under the conditions listed above.

*** Fraused Rats may be kept at a higher stocking in the short period after the period of disrepulsion until such time as the animals are placed on trial-provided that the animals are placed in major experimental animal health systems with sufficient environmental enrichment. Liability shall not result in the loss of welfare, such as increased aggregate, disease or mortality level, other behavioural abnormality, weight loss or other physiological or behavioural stress reactions, including stereotypical pees.

Table 3. Minimum requirements for the termination of desert rats

Table 4. Minimum requirements for the housing of hamsters

* Fraused to beekeepers may be kept at a higher stocking in the short period after resettlement until such time as the animals are placed on the test-provided that the animals are placed in major experimental animals with sufficient environmental enrichment. Liability shall not result in the loss of welfare, such as increased aggregate, disease or mortality level, other behavioural abnormality, weight loss or other physiological or behavioural stress reactions, including stereotypical pees.

Table 5. Minimum requirements for the installation of guinea pigs

However, the height of the guinea pig cage shall be sufficient to ensure that the guinea pigs in the cage in question can sit up and grooves.

2.1.4.4. Feeding

The form, content and appearance of the feed must satisfy the nutritional and behavioural needs of the rodents ; it must be given to wholesated feed as needed.

The feed must be bidder and without contamination. For the selection of raw materials, manufacture, preparation and award of the feed, measures must be taken to minimise chemical, physical and microbiological contamination. The feed shall not be used after the expiry date.

Packaging, transport and storage of the feed must be carried out in a manner so that pollution, degradation and destruction of the feed must be avoided. The storeroom must be chilled, dark, dry, and safe from pests and insects. Literally food, such as vegetables, fruit, meat, fish, etc. must be stored in chilling rooms, refrigerators or freezers.

All fodder, treng, and other tools used for feeding shall be cleaned periodically and sterilized if necessary. If wet fodder is used, or if the fodder is easily contaminated with water, urine and osvs, cleaning must be carried out on a daily basis.

Each rodent must have access to the feed, and there must be sufficient feeding space to restrict the feeding of the fodder competition. Where necessary, the feed recording must be carried out in order to avoid overweight or malnutrition.

2.1.4.5. Horizontal

There must always be clean drinking water available to all rodents. The supply of water must be established so that the risk of contamination of micro-organisms is minimised.

The water supply facilities must be designed and must be used, so that adequate quality water is provided. There must be sufficient number of beverages to be taken by the number of rodents who have access to them. Where automatic water-supply installations are used, their function shall be controlled, as well as maintaining and periodically rinsed in order to avoid accidents such as the stop or leakage, and the spread of infections. If cages are used with solid bottom, care must be taken to minimise the risk of flooding.

2.1.4.6. Substrator, stockings and nesting

There must be solid or perforated ground with sock in the cages. In the final part of the drainness, childbirth and the advice of breeding, breeding houses must be kept only on solid floors by stocking.

Appropriate stockings and resting places must always be taken to the rodent ; the same shall apply to appropriate exec or orifice for breeding animals.

Appropriate materials must be placed in cages, etc. to absorb urine and feces, give the rodent the chance to show certain species-specific behavioural forms, such as the ourach, diggity or establishment of underground corridors, arrange for a convenient commuter ; the surface or a secure area to rest and make it possible for the rodent to build to bear children.

The materials must be dry, absorbent, dust-free, free of toxic and free from infectious micro-organisms or vermin and other forms of contamination. Materials on the basis of wood which are chemically treated or contain toxic, natural substances, and products which are not clearly defined and standardised may not be used.

The bunting of the cage shall provide a fixed and convenient resting area for all rodents. All rest areas shall be kept clean and dry.

2.1.4.7. Cleaning

A very high standard should be maintained for cleanliness and order in housing, washing and storage rooms. Adequate routines for cleaning, washing, decontamination and, where appropriate, sterilization of cages and accessories, bottles and other equipment shall be provided and implemented.

Cleaning and disinfecting systems must be suitable for the rodent and must not be harmful to their health or welfare.

In the case of cleaning of the experimental livestock, some of the gnawed scented markings shall be left behind. This does not, however, apply to the cleaning of experimental animals, where the other rodents must subsequently be placed other rodents than those placed in the installation before cleaning.

The animals must not be subjected to frequent changes in their experimental animal health systems.

Proper females with pregnant females and females must only be changed when there are weighty reasons for this.

The decision on how often to be cleaned must be taken, taking into account the caged species, the nature of the animal, the stocking density and the capacity of the ventilation systems to maintain adequate air quality.

2.1.4.8. Handling

In handling, the gnawing and burying of the cage must be disrupt at least. This is particularly important to hamsters.

2.2. Rabbits (Oryctolagus cuniculus)

2.2.1. Introduction

This Annex lays down a series of requirements for the termination of rabbits used or used for experiments.

The minimum requirements laid down are minimum requirements to ensure that the well-being of the rabbits is best taken into account in the concrete conditions.

2.2.2. Climatic conditions, etc.

2.2.2.1. Ventilation

It shall be provided for adequate ventilation in the cleanup rooms and in the experimental livestock areas so that the needs of the canines are met. The ventilation system must be able to provide adequate fresh air of suitable quality, remove excess heat and humidity, and keep the quantity and spread of smelling, toxic gases, dust and infectious substances of any kind down.

Unless special circumstances apply, or natural ventilation by mechanical ventilation, air shall be carried out 15 to 20 times per. hour in the enclosure room. However, at low levels of stocking, the air shift may be reduced to 8 to 10 times per year. Hour.

It must be ensured that there is no harmful recirculation of untreated air.

The ventilation system must be installed in such a way as to avoid harmful features and noise nuisance.

Smoking is not allowed in rooms where the rabbits are in place.

2.2.2.2. Temperature

Rabbits must be kept within a temperature range between 15 and 21 ° C. Among the groups of rabbits in caged cages, the temperature must be measured by the stocking in the cage, unless it is considered inflambable to assume that the room temperature does not differ significantly from the temperature in the housing room.

The temperature in solitary confinement must be particularly carefully monitored.

2.2.2.3. Humidity

The relative humidity in cininatinator plants must be at least 45%.

2.2.2.4. Lighting

In cases where daylight does not constitute a suitable light / dark cycle, artificial lighting shall be used in such a way as to satisfy the biological needs of the rabbits. Rabbits must not be subjected to a strong light unless it can be demonstrated that the species in question is not harmful, and there must be darker areas available in the exploratory conditions in which the rabbits can withstand.

There must be light enough to carry out the general operation and to the rabbits. It must be made sure that regular light-and dark times of the rabbit are appropriate-and there must be no unnecessary interruptions to these candles and dark times.

In the case of a team of albinos, particular account must be taken of their sensitivity towards light.

2.2.2.5. Noise

High and sudden noises must be avoided as far as possible in the cleanup rooms.

Noise levels within the rabbit ' s area of reaping, including in certain cases, ultrasound shall be minimized-in particular during the period of rest of the animal.

In case of an abnormal behaviour or an abnormal breeding pattern, due to ultrasonic noise, the acoustic environment must be monitored over a wide spectrum spectrum until the source of the noise has been identified, or the cause of the abnormal behaviour or the abnormal breeding pattern of other means has been determined.

Alarm systems must make noise as far as possible outside of the rabbit territory. The design of premises and pales must be designed in such a way as to ensure that the unhealthy environment is not harmful to the rabbits. The accommodation rooms must be provided with adequate light-disc and absorption materials.

2.2.2.6. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Heat, ventilation, cooling and bemoiling plants and similar installations which are significant for the welfare of the animals must be provided with monitoring devices and alarms to ensure that any possible errors can quickly be registered and remedied immediately.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care must be taken to ensure that the operation of the alarm systems disrupts the animals as little as possible.

2.2.3. Health

An action plan shall be provided by institutions or undertakings to ensure that an appropriate state of health is maintained, which protects the well-being of the rabbits and meets the scientific requirements ; this action plan must include a microbiological surveillance programme and plans for the handling of major health problems, as well as the definition of health parameters and the way in which the arrival of new rabbits is defined.

The person responsible for the testing animals shall ensure regular supervision of the rabbits and supervision of their accommodation and care. The supervision must be carried out by a veterinarian or a corresponding competent person.

Health monitoring must be carried out regularly.

hygienic procedures must be laid down to ensure that the potential risk of infection for rabbits and staff to deal with the rabbits involves minimising the maximum possible level.

2.2.4. Astining, etc.

2.2.4.1. Restining

Young rabbits and females are to be housed in harmonics, social groups. Individual crop production may only be carried out if it is justified by veterinary or welfare reasons.

Adults that display or may be expected to display territorial conduct must not be allowed to be housed with other uncastrated males. Young rabbits and adult females must as far as possible be lashed in enriched secession systems.

If it is to be expected that the group-stabled rabbits show aggressive behaviour towards one another, the groups must be monitored.

After the weaning, the rabbits from the same envelope shall, incidentally, be housed together as possible.

Where rabbits cannot be disbarred into groups, they must be placed in close visual contact with other rabbits.

2.2.4.2. Environmental enrichment

The channelling of environmental enrichment in the form of growfed, blocks of hay or chewes and an area shall be allocated to it. The group housing of rabbits must be provided for visible barriers and directions where the rabbits can seek refuge and keep a lookout.

Rabbits need to hide in the form of boxes or something. For breeding houses, we also need to mourn for the nesting materials and an exposition box.

The cage must, as far as possible, be rectangular. The cage is to be taken care of in the case of a swolted area. This shelf must be big enough for the rabbit to lie or sit on it and unswelled move under it, and it must not cover more than 40%. by the gold warehouse. The Burridge must be sufficient to ensure that the rabbit can sit upright, without its ears affecting the ceiling of the agency. However, this does not apply to free heights and shelves.

In the special cases where scientific or veterinary reasons speak in order to omit the shelf, the scope of the agency shall be 33%. greater for a single rabbit and 60%. greater for 2 rabbits so that the rabbit's freedom of movement is being facilited and a better chance of escaping a more dominant rabbit. Rabbits must be kept in the secession as far as possible.

At least three to four days before the expected birth, females must appear to be in an extra room or a nesting box where they can build a nest. The straw or other exhales must be taken.

Bure that houses females with young must be so designed that the female can move to another room or a shelf away from her young after they have left the nest. After the weaning, siblings of the same culd shall be kept in the breeding cage as long as possible.

2.2.4.3. Dimensions

The bundareal area is understood in all tables freely available in the bundaarea that the rabbits can move-that is, the bunnies. where permanent fixtures such as feed bowls and similar do not prevent the use of the enclosure area-and when calculating the minimum area, any spaces in nesting boxes and on top of shelves, boxes and so on shall not be included.

The following and subsequent tables with minimum requirements for the termination of rabbits means "cage height" the vertical distance between the floor or the bottom of the floor and the upper horizontal part of the lid or the cage.

Table 1. Minimum requirements for the housing of rabbits older than 10 weeks

The table applies to cages and secession. In the cages of cages, a shelf must be taken (see Table 4). The operating system must contain any information that subdivides space so that the rabbits can initiate or avoid social contact as needed.

If more than two rabbits are disbarred together, the second four rabbits shall be attributed to the minimum 3000 cm2 per. rabbit, while for each additional rabbit, it must be given at least 2500 cm2.

Table 2. Minimum requirements for the starvation of females and chilsisiblings up to 10 weeks

The table applies to the starvation of females with young and for the disrepulsion of carbs after wewing.

There may be eight siblings from the same cutter in the stool from the westage, to the age of seven weeks. When the kids are 8 to 10 weeks old, five siblings can be held at the minimum floor area.

Table 3. Minimum requirements for the housing of rabbits younger than 10 weeks

The table applies where the rabbit cubs are placed in conjunction with other rabbit kids, which are not their siblings from the cold.

The table must be used for both cages and secession. The operating system must contain any information that subdivides space so that the rabbits can initiate or avoid social contact as needed.

Table 4. Dimensions for shelves in rabbit cages

In order to optimize the use of the shelf and the cage, the circumtrable size of the shelf may only deviate from the specified targets with ± 5-10%.

2.2.4.4. Feeding

The form, content and appearance of the feed must satisfy the nutritional and behavioral needs of the rabbit food ; it must be granted to feed as needed.

The feed must be bidder and without contamination. For the selection of raw materials, manufacture, preparation and award of the feed, measures must be taken to minimise chemical, physical and microbiological contamination. The feed shall not be used after the expiry date.

Packaging, transport and storage of the feed must be carried out in a manner so that pollution, degradation and destruction of the feed must be avoided. The storeroom must be chilled, dark, dry, and safe from pests and insects. Literally food, such as vegetables, fruit, meat, fish and so on must be kept in refrigerant, refrigerators or freezers.

All fodder, treng, and other tools used for feeding shall be cleaned periodically and sterilized if necessary. If wet fodder is used, or if the fodder is easily contaminated with water, urine and osvs, cleaning must be carried out on a daily basis.

Each rabbit must have access to the feed and there must be sufficient feeding space to restrict the feeding of the fodder competition. Where necessary, the feed recording must be carried out in order to avoid overweight or malnutrition.

2.2.4.5. Horizontal

There must always be clean drinking water available to all rabbits. The supply of water must be established so that the risk of contamination of micro-organisms is minimised.

The water supply facilities must be designed and must be used, so that adequate quality water is provided. There must be sufficient number of beverages to be taken after the number of rabbits which have access to them. Where automatic water-supply installations are used, their function shall be controlled, as well as maintaining and periodically rinsed in order to avoid accidents such as the stop or leakage, and the spread of infections. If cages are used with solid bottom, care must be taken to minimise the risk of flooding.

2.2.4.6. Substrator, stockings and nesting

Grid floors and floors of wireless must not be used without a rest area with solid or perforated floor with a stocking which is large enough to accommodate all the rabbits in one time.

If grid floors or floors of wireless in part of the cage are used, the bottom must be covered by a thick layer of straw or similar.

Appropriate stockings and resting places must always be taken to the rabbits, the same shall apply to suitable exposition material or orifice for breeding rabbits.

Appropriate materials must be placed in cages, etc. to absorb urine and feces, give the rabbits the ability to expel certain types of species, such as the ouraging, dignifying behaviour or the establishment of underground corridors, arrange for a convenient commuter ; the surface or a secure area to rest and make it possible for the rabbit rabbits to build ready to bear children.

The materials must be dry, absorbent, dust-free, free of toxic and free from infectious micro-organisms or vermin and other forms of contamination. Materials on the basis of wood which are chemically treated or contain toxic, natural substances, and products which are not clearly defined and standardised may not be used.

The bunden of the cage shall provide a fixed and convenient resting area for all rabbits. All rest areas shall be kept clean and dry.

2.2.4.7. Cleaning

A very high standard should be maintained for cleanliness and order in housing, washing and storage rooms. Adequate routines for cleaning, washing, decontamination and, where appropriate, sterilization of cages and accessories, bottles and other equipment shall be provided and implemented.

Cleaning and disinfecting systems must be appropriate to the rabbits and must not be harmful to their health or wellbeing. There must be clear operational procedures-including registration-for the exchange of sock in the curre of the rabbit ' s bulb.

There must be regular cleaning and, as appropriate, the replacement of the material that is the bottom of the rabbit's cages, so as to prevent it becoming a source of infection and attacks on parasites.

In the cleaning of cages, account must be taken of the behavioral needs of the rabbits, including the impact of the odourings for social relations, etc. Decision on how often to be cleaned must be taken, taking into account the experimental animal health system, the stocking density and the capacity of the ventilation systems to maintain adequate air quality.

2.2.4.8. Handling mv.

The rabbits must, as far as possible, be possible for exercise.

The rabbits must, as far as possible, become accustomed to handling during routine operation and during tests. Handling can be avoided, however, if special circumstances apply, including when the rabbit is caught in nature.

The staff shall talk to, handle, train with, and softer the rabbits as needed.

2.3. Fritter (Putorius putorius)

2.3.1. Introduction

This Annex lays down a series of requirements for the installation of ferrets used or should be used for tests.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the free-step is best taken on the basis of the specific conditions.

2.3.2. Climatic conditions, etc.

2.3.2.1. Ventilation

It shall be provided for adequate ventilation in the cleanup rooms and in the experimental livestock areas so that the needs of the cultivated free tters are met. The ventilation system must be able to provide adequate fresh air of suitable quality, remove excess heat and humidity, and keep the quantity and spread of smelling, toxic gases, dust and infectious substances of any kind down.

Unless special circumstances apply, or natural ventilation by mechanical ventilation, air shall be carried out 15 to 20 times per. hour in the enclosure room. However, at low levels of stocking, the air shift may be reduced to 8 to 10 times per year. Hour.

It must be ensured that there is no harmful recirculation of untreated air.

The ventilation system must be installed in such a way as to avoid harmful features and noise nuisance.

Smoking is not permitted in rooms where the feral ferrets are at home.

2.3.2.2. Temperature

Frits must be kept in a temperature range between 15 and 24 ° C.

2.3.2.3. Lighting

It must be ensured that the illumination does not bother the ferrets. The top shelf shall be tightened up if necessary.

Exemptions shall, as far as possible, have access to natural light.

Fritters must have the light and dark times of the kind for the species necessary length. Releasing non-housing in the light of the natural 24-hour light cycle shall have at least 8 and maximum 16 hours of artificial lighting in the context of the day.

However, if necessary to steer the reproductive cycle of free the proposed period, the period of the period may be limited to 6 hours.

If natural lighting is not possible, then the weak nature of night tels (5-10 lux) must be taken to preserve a certain view and to take account of their reflex reflex ("Startle reflex").

2.3.2.4. Noise

The ferrets must be shielded from vibrations as well as sudden and high, unfamiliar noises, including noises stemmed from the operation of the place of installation.

Noise from the storage site shall be reduced by means of sound absorbing materials and / or constructs.

Fritter must not be the housing in rooms that are completely without noise or other sound.

2.3.2.5. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Heat, ventilation, cooling and bemoiling plants and similar installations which are significant for the welfare of the animals must be provided with monitoring devices and alarms to ensure that any possible errors can quickly be registered and remedied immediately.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care must be taken to ensure that the operation of the alarm systems disrupts the animals as little as possible.

2.3.3. Health

An action plan shall be provided by institutions or undertakings to ensure that an appropriate state of health is maintained, which protects the well-being of the Frits and meets the scientific requirements ; this action plan must include : microbiological surveillance programme and plans for the handling of major health problems, as well as the definition of health parameters and procedures for the receipt of new ferrets.

The person responsible for the test animals shall ensure a regular inspection of the ferrets and monitoring of their accommodation and care. The supervision must be carried out by a veterinarian or a corresponding competent person.

Health monitoring must be carried out regularly.

hygienic procedures must be laid down to ensure that the potential risk of infection for ferrets and staff to deal with the free will be reduced as much as possible.

2.3.4. Astining, etc.

2.3.4.1. Restining

The design or modification of ferrets must be carried out in accordance with updated, recognised practical and scientific experience on the physiological, behavioural and health requirements of the free-stage ;

The Frits must, unless for welfare reasons speak against, be placed in larger, social harmonious groups.

The veterinary carers, including the separating walls between these, must be robust and easy to clean. Design and design shall provide a fresh and illuminating environment that gives the animals a good outlook to other ferrets and staff elsewhere in the establishment. There must be an opportunity for the ferrets to seek refuge and disruption in their own cage-and where they cannot be seen by ferrets in other cages.

The construction of the cage must be such that if they try to escape or harm themselves, the free man cannot escape or harm himself.

Killer females may be placed in the end of the last part of the drainage period, however, at the earliest two weeks before birth.

It must be ensured that the additional needs of the single-free from the individual needs of increased welfare and care are taken into account, including, on a daily basis, for additional human contact and visual, auditory and as far as possible physical contact with other ferrets.

2.3.4.2. Socialization

The time limit for handling must be adjusted as early as possible. The handle must be carried out on a daily basis.

2.3.4.3. Environmental enrichment

The formulation of the experimental vets must satisfy the species and breeding-specific needs of the free-species and breeding.

The design of the experimental vets must allow for a certain element of disturbance for the ferrets and allow them to exercise a degree of self-control over their social contact.

In addition to the minimum number of floors described below, separate areas for different activities such as swolled platforms / shelves and sub-divisions of the plant must be provided for separate areas. Redeboxes must be designed in such a way that they can accommodate the ferrous cubs within the nest.

Environmental enrichment must be allocated, for example, in the form of containers, pipes, paper bags and water baths / bowls.

2.3.4.4. Dimensions

The minimum height of the cage must be 50 cm.

The floor must be large enough to allow the ferret to move around and to allow the ferret to choose different areas to sleep and eat, and to urination / feces.

In order to increase the access of free speakers to move, the cages / facilities of the free-premises must, as far as possible, be rectangular.

For the purposes of floor space, both tables are freely available for the floor to which the ferrets are able to move ; that is, where fixtures such as feed bowls, shelves and climbing statives are not obstructing the use of the gold palette-and at the calculation of the minimum area, any areas on top of shelves or similar may not be taken into account.

For individual crops, the ferret must have at least 4500 cm2 free-accessible floor space.

Table 1. Minimum requirements for the group housing of ferrets

*Since there must be room for environmental enrichment, no cage may be less than 4500 cm2.

Table 2. Minimum requirements for the group housing of grown males and females with young

2.3.4.5. Feeding

The form, content and appearance of the feed shall satisfy the nutritional and behavioral needs of the free-room.

The feed must be bidder and without contamination. For the selection of raw materials, manufacture, preparation and award of the feed, measures must be taken to minimise chemical, physical and microbiological contamination. The feed shall not be used after the expiry date.

Packaging, transport and storage of the feed must be carried out in a manner so that pollution, degradation and destruction of the feed must be avoided. The storeroom must be chilled, dark, dry, and safe from pests and insects. Literally food, such as vegetables, fruit, meat, fish, etc. must be stored in chilling rooms, refrigerators or freezers.

All fodder, treng, and other tools used for feeding shall be cleaned periodically and sterilized if necessary. If wet fodder is used, or if the fodder is easily contaminated with water, urine and osvs, cleaning must be carried out on a daily basis.

Each ferret shall have access to the feed and there shall be sufficient feeding space to restrict the feeding of the fodder competition. Where necessary, the feed recording must be carried out in order to avoid overweight or malnutrition.

2.3.4.6. Horizontal

There must always be clean drinking water available to all ferrets. The supply of water must be established so that the risk of contamination of micro-organisms is minimised.

The water supply facilities must be designed and must be used, so that adequate quality water is provided. There must be sufficient drinking points to be taken by the number of ferrets that have access to them. Where automatic water-supply installations are used, their function shall be controlled, as well as maintaining and periodically rinsed in order to avoid accidents such as the stop or leakage, and the spread of infections. If cages are used with solid bottom, care must be taken to minimise the risk of flooding.

2.3.4.7. Substrator, stockings and nesting

The floor in the cage of the free cages must be a solid floor with smooth, crimnied surface. There must be access to additional equipment, such as nesting boxes or platforms, where all ferrets can be reside.

Open floor systems such as grids or wire networks may not be used for ferrets.

The cages must include litter and necks in the form of hay, straw, paper or other forms of hay.

2.3.4.8. Cleaning

A very high standard should be maintained for cleanliness and order in housing, washing and storage rooms. Adequate routines for cleaning, washing, decontamination and, where appropriate, sterilization of cages and accessories, bottles and other equipment shall be provided and implemented.

Cleaning and disinfecting systems shall be appropriate for the stainers and shall not be harmful to their health or wellbeing.

Cleaning in the form of the pooling of the stainers ' facilities shall be carried out when necessary. When the stains ' facilities are spouted, the stains are to be moved to a dry place so that they do not get wet. They must first be put back when they're pretty dry.

If the bowels of free products create hygienic problems, the cage shall be equipped with waste baskets.

The veterinary carers must be cleansed at least once a day. All excrement and coiled materials must be emptied daily and, where necessary, more often-be emptied out of the trays and / or removed from all other areas used by the ferrets.

2.3.4.9. Handling mv.

The ferrets must, as far as possible, be possible for exercise.

The time limits shall be adjusted as far as possible to handling during routine operation and during tests. Handling can be avoided, however, if special circumstances apply, including those of ferrets trapped in nature.

The staff shall speak to, handle, train with and soignite ferrets as needed.

2.4. Cats (Felis catus)

2.4.1. Introduction

This Annex lays down a series of requirements for the termination of the use of cats used or should be used for testing.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the cat's well-being is best taken from the specific conditions.

2.4.2. The climatic conditions, the mv.

2.4.2.1. Ventilation

Adequate ventilation must be provided in the storage rooms and in the experimental livestock areas so as to meet the needs of the intended cateners. The ventilation system must be able to provide adequate fresh air of suitable quality, remove excess heat and humidity, and keep the quantity and spread of smelling, toxic gases, dust and infectious substances of any kind down.

Unless special circumstances apply, or natural ventilation by mechanical ventilation, air shall be carried out 15 to 20 times per. hour in the enclosure room. However, at low levels of stocking, the air shift may be reduced to 8 to 10 times per year. Hour.

It must be ensured that there is no harmful recirculation of untreated air.

The ventilation system must be installed in such a way as to avoid harmful features and noise nuisance.

Smoking is not allowed in rooms where the cats are in place.

2.4.2.2. Temperature

In the case of cats in experiments, the temperature must be maintained between 15 and 21 ° C. Cats that are not in tests must be kept within a temperature spectrum that does not harm their welfare.

Until newborn kittens have achieved full internal temperature regulation (typically after approximately 20%). In ten days ' time, they must be given extra heat in the food safety deposit box.

2.4.2.3. Lighting

Cats should have access to natural light as far as possible.

If cats are not kept by the natural 24-hour light / dark cycle, they shall be given artificial lighting so that they can maintain normal activity and conduct. The light part of the day must in these cases be between 10 and 12 hours.

If natural lighting is not possible, then there is a need for a feeble night tellary (5-10 lux).

2.4.2.4. Noise

Noise levels in the zone ' s hearing range, including in certain cases, ultrasound shall be minimized-in particular during the period of rest of the animal.

Alarm systems must, as far as possible, make sound out of the cathearised territory of the cat. The design of premises and pales must be designed in such a way that the acoustic environment does not harm the cattles. The accommodation rooms must be provided with adequate light-disc and absorption materials.

2.4.2.5. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Heat, ventilation, cooling and bemoiling plants and similar installations which are significant for the welfare of the animals must be provided with monitoring devices and alarms to ensure that any possible errors can quickly be registered and remedied immediately.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care must be taken to ensure that the operation of the alarm systems disrupts the animals as little as possible.

2.4.3. Health

An action plan shall be provided by institutions or undertakings to ensure that an appropriate state of health is maintained, which protects the well-being of the cat and meets the scientific requirements ; this action plan must include : microbiological surveillance programme and plans for the handling of major health problems, as well as the definition of health parameters and procedures for the reception of new cats.

The person responsible for testing animals shall ensure a regular monitoring of the products and monitoring of their accommodation and accommodation ; this must be carried out by a veterinarian or an equivalent competent person.

Health monitoring must be carried out regularly.

hygienic procedures must be laid down to ensure that the potential risk of infection for cats and staff to deal with the cats is reduced as far as possible.

2.4.4. Astining, etc.

2.4.4.1. Restining

In accordance with updated practical and scientific experience on the physiological, behavioural and health requirements of the Kattenes, the construction or modification of the litigation conditions must be carried out in accordance with updated practical and scientific experience.

The veterinary penalties, including any separable separators, must be robustaged and easy to clean. In the design and design of their design, they shall be as open and light as possible for the cats to have a good view of their own and any surrounding experimental animal health facilities.

Huncats and neutralized cats of both sexes must be in social harmony between groups of up to 12 cats.

The setting up of groups must monitor closely the behaviour of all the individuals in the group. When cats are regrouped, or when a foreign cat is introduced into a group, it must be ensured that the group will still be able to function.

Special vigilance must be shown when malcastrated males are in the hands of each other. The same applies when cats are disbarred in larger groups.

Cats must not be disbarred for more than 24 hours, without it being well-founded on welfare or veterinary reasons.

Cats that have repeatedly shown aggressive behaviour towards other cats can only be placed on their own if there is no suitable species of species. The social load among all individuals who are positioned in pairs or in groups must be carefully monitored and, at least, weekly. In this respect, behavioric and stress studies must be carried out.

Huncats in the last two weeks of the killing or with kittens in less than four weeks can be stained alone. During this period, as far as possible, female cats, who usually live in a group, must be given access to their group-for example, by connecting the caged of the female and the group of the group.

2.4.4.2. Socialization

Sources in the ages of 2 to 8 weeks must have social contact with their siblings from the cold and, as far as possible, with adult cats (e.g., mother) and people. The kittens must, as far as possible, get used to the conditions that they are likely to meet in the future.

The sources must be adjusted to daily handling as soon as possible from the first day of birth.

Every cat must have a period of time to play and commonplace, socially consenting with people, and a further time for regular soigning / care. In the case of cat litter alone, time for further human contact must be set aside.

2.4.4.3. Environmental enrichment

The plant culture plant must be equipped with swolored, partially closed boxes / reder (e.g. a shelf around 1 metres above the floor with three walls and a roof), which allows the cat to look at the surrounding environment and when housing takes place in groups-to keep a convenient distance from other cats. There must be so many of these crates / replaces that there will be no serious fighting for the seats. The crates / shipowners must be divided into the cage so that the crates can make full use of the area available.

There must be an opportunity for the cats to be able to seek refuge and to be undisturbed within their own experimental animal health system and, in particular, out of sight of cats in other experimental animal health systems. It shall be taken for vertical seats or similar timber so that the cats can hiss claws and depose scented marks.

The cats must be encouraged to play, and there must be a choice of enrichment / toys that are to be replaced regularly. The cattlecats must be able to use their natural hunting instincts to the greatest extent possible.

2.4.4.4. Outdoor lions.

Outdoor lions will have to be available as far as possible.

Cats may never be forced to spend their entire lives outdoors, and they must at all times have the possibility of staying indoors under conditions that meet the requirements of this Annex to the design and environmental control requirements of this Annex.

The floor of an outdoor lion has to be easy to clean and must not be able to harm the cats.

2.4.4.5. Dimensions

The minimum age for a female cat and its kittens are-until the weaning-the same as for a single cat, but must be progressively increased so that the female cat and kittens are lagged within four months at the latest in areas corresponding to the requirements of adult animals. The normal age for wetings of wets is 7 to 12 weeks.

For the purposes of this table, the floor area is as free the floor area in which the cats can move-that is, the floor area. where fixtures such as feed bowls and shelves do not obstruate the use of the floor area-and when calculating the minimum area, any land on the shelf and the like may not be included.

Table 1. Minimum requirements for the termination of cats

2.4.4.6. Floor Occupancy

Kattearre mv must be fitted with a solid floor with a smooth and cribed stocking. For all the cats, a comfortable resting place must be provided for the use of special environmental enrichment.

Carve comprising or wireless or wireless shall not be used for cats.

2.4.4.7. Feeding

The form, content and appearance of the feed must satisfy the nutritional and behavioural needs of the cat.

The feed must be bidder and without contamination. For the selection of raw materials, manufacture, preparation and award of the feed, measures must be taken to minimise chemical, physical and microbiological contamination. The feed shall not be used after the expiry date.

Packaging, transport and storage of the feed must be carried out in a manner so that pollution, degradation and destruction of the feed must be avoided. The storeroom must be chilled, dark, dry, and safe from pests and insects. Literally food, such as vegetables, fruit, meat, fish, etc. must be stored in chilling rooms, refrigerators or freezers.

All fodder, treng, and other tools used for feeding shall be cleaned periodically and sterilized if necessary. If wet fodder is used, or if the fodder is easily contaminated with water, urine and osvs, cleaning must be carried out on a daily basis.

Each cat shall have access to the feed and there must be sufficient feeding space to restrict the feeding of the fodder competition. Where necessary, the feed recording must be carried out in order to avoid overweight or malnutrition.

The foam bowls must be at least 0,5 metres from the litter box and shall not be switched on.

2.4.4.8. Horizontal

There must always be clean drinking water available to all cats. The supply of water must be established so that the risk of contamination of micro-organisms is minimised.

The water supply facilities must be designed and must be used, so that adequate quality water is provided. There must be sufficient drinking points to be taken by the number of cats that have access to them. Where automatic water-supply installations are used, their function shall be controlled, as well as maintaining and periodically rinsed in order to avoid accidents such as the stop or leakage, and the spread of infections. If cages are used with solid bottom, care must be taken to minimise the risk of flooding.

2.4.4.9. Catbaths and rest spaces, etc.

For each two cats, there must be at least one litter box to measure at least 300 x 400 mm and containing an appropriate, absorbent and untoxic cattegous material or similar to which the cattles can and will use. If urine and feces are regularly disposed of outside the hills, the extra-trays must be provided with a different type of cat litter or the like. If this is inert by cats that are in pairs or groups, this is a sign of social incompatibility, and cats have to be removed from one after you until the problem is resolved.

There must be resting seats available for all the cats. They must be manufactured from a material that is easy to clean and must contain an appropriate and soft material such as polyester fleece or similar.

2.4.4.10. Cleaning

A very high standard should be maintained for cleanliness and order in housing, washing and storage rooms. Adequate routines for cleaning, washing, decontamination and, where appropriate, sterilization of cages and accessories, bottles and other equipment shall be provided and implemented.

Cleaning and disinfecting systems must be appropriate for the cats and must not be harmful to their health or wellbeing.

The veterinary sanctity must be cleaned at least once a day. The carts must be emptied on a daily basis, and a new catart must be filled in.

Cleaning-up of the experimental animals must not cause the cats to get wet. When the experimental animals are being spouted, the cattlees must be moved to a dry place. They must first be put back when they're pretty dry.

2.4.4.11. Handling mv.

The cattmen must be able to exercise the scope for exercise.

The cattles must, as far as possible, become accustomed to handling during routine operation and during tests.

Handling can, however, be avoided if special circumstances apply, including in the exceptional cases where a cat is trapped in the wild.

The staff shall talk to, handle, train with and soignate the cats as needed.

2.5. Canis familiaris (hounds)

2.5.1. Introduction

This Annex lays down a series of requirements for the installation of dogs used or should be used for testing.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the dog is best taken on the basis of the specific conditions.

The specified requirements apply to beagles. Where other races are used, account shall be taken of the individual ravasaries.

2.5.2. Climatic conditions, etc.

2.5.2.1. Ventilation

It shall be provided for adequate ventilation in the cleanup rooms and in the experimental livestock areas so as to meet the needs of the starved female. The ventilation system must be able to provide adequate fresh air of suitable quality, remove excess heat and humidity, and keep the quantity and spread of smelling, toxic gases, dust and infectious substances of any kind down.

Unless special circumstances apply, or natural ventilation by mechanical ventilation, air shall be carried out 15 to 20 times per. hour in the enclosure room. However, at low levels of stocking, the air shift may be reduced to 8 to 10 times per year. Hour.

It must be ensured that there is no harmful recirculation of untreated air.

The ventilation system must be installed in such a way as to avoid harmful features and noise nuisance.

Smoking is not allowed in rooms where there are stables of dogs.

2.5.2.2. Temperature

For dogs in experiments, the temperature must be maintained between 15 and 21 ° C. Dogs that are not in tests must be kept within a temperature spectrum that does not harm their well-being.

Until newborn puppies have achieved full internal temperature regulation (typically after approximately 20%). In ten days ' time, they must be given extra heat in the food safety deposit box.

2.5.2.3. Lighting

Dogs must have access to natural light as far as possible.

If dogs are not kept by the natural 24-hour light / dark cycle, they shall be given artificial lighting to maintain normal activity and behaviour. The light part of the day must in these cases be between 10 and 12 hours.

If natural lighting is not possible, then there is a need for a feeble night tellary (5-10 lux).

2.5.2.4. Noise

Noise in the enclosure room, including noise in the form of the feral bark, which may lead to the deterioration of the health or wellbeing of the dogs and any noise source that can stimulate further manure must be limited as much as possible.

If necessary, noise resulting from the cleanup site must be reduced if necessary by sound absorbing materials or constructs.

2.5.2.5. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Heat, ventilation, cooling and bemoiling plants and similar installations which are significant for the welfare of the animals must be provided with monitoring devices and alarms to ensure that any possible errors can quickly be registered and remedied immediately.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care must be taken to ensure that the operation of the alarm systems disrupts the animals as little as possible.

2.5.3. Health

An action plan shall be provided by institutions or undertakings to ensure that an appropriate state of health is maintained that protects the well-being of the species and meets the scientific requirements ; this action plan must include : microbiological surveillance programme and plans for the handling of major health problems, as well as the definition of health parameters and procedures for the reception of new dogs.

The person responsible for the testing animals shall ensure regular supervision of the dogs and monitoring of their accommodation and care ; this must be carried out by a veterinarian or an equivalent competent person.

Health monitoring must be carried out regularly.

hygienic procedures must be laid down to ensure that the potential risk of infection for dogs and staff to deal with the hounds shall be reduced as much as possible.

2.5.4. Astining, etc.

2.5.4.1. Restining

The design or modification of dog-keeping conditions for dogs shall be carried out in accordance with updated practical and scientific experience on the physiological, behavioural and health requirements of the female ;

The veterinary penalties, including any separable separators, must be robustaged and easy to clean. In the design and design of their design, they shall be as open and light as possible, giving the dogs a good view of other dogs and to the staff outside their own facilities.

Dogs must be disbarred in social harmony-and, as far as possible, in larger groups, unless welfare requirements make this impossible. When dogs are being regrouped, or when a foreign dog is introduced into a group, it must be ensured that the group will still be able to function. In all cases, the groups must be regularly monitored for social compatibility.

Dogs cannot be placed on their own for more than four hours, without it being well-founded on welfare or veterinary reasons.

It must be ensured that the particular needs of single-placed female needs for increased welfare and care are taken into account, including, on a daily basis, for additional human contact and visual, auditory and as far as possible physical contact with other dogs.

Without prejudice to veterinary or welfare reasons, dogs are to be provided on their own, on a daily basis, with the possibility of exercise in a separate area-if possible, together with other dogs. This must be done in interaction with the staff and under the supervision of this.

Avlshanner must, if possible, be stored in socially harmonious or groups or in a number of carpets.

Killer bitches must first be moved to the food booth between one and two weeks before the expected birth. While they're in the food vault, they have to have extra human contact on a daily basis.

2.5.4.2. Socialization

In the age of 4 to 20 weeks, the male age of 4 to 20 weeks shall have social contact with its siblings from the cold, grown-up dogs (e.g. the mother) and human beings. The popples must, as far as possible, become accustomed to the conditions that they will probably face in the future.

The popples shall be adjusted to daily handling as soon as possible from the first day of birth.

2.5.4.3. Environmental enrichment

The design of indoor and exterior experimental animals must give the dogs a certain element of disorder and enable them to exercise a degree of control over their social interaction and the environment of mhp. to make choices to reduce the stress-related behaviour.

Separate areas must be taken to different activities.

Dogs must have free access to swolored platforms or similar devices that are set up in a height that allows the dog to go up to and under the platform.

Dogs must be given, they can use to chew on.

In the absence of veterinary reasons, dogs must at least once a day be moved to a separate area and be allowed to exercise-if possible, together with other dogs. This must be done in interaction with the staff and under the supervision of this.

2.5.4.4. Outdoor lions.

Outdoor lions will have to be available as far as possible.

Dogs must never be forced to spend their entire lives outdoors, and they must at all times have access to an indoor plant that meets the requirements of the design and environmental control, etc. The indoor plant must be present ; at least 50%. of the minimum space to be made available to the dogs, cf. Table 1 below.

The floor of an outdoor lion shall be easy to clean and must not be able to harm the hounds.

2.5.4.5. Dimensions

The space allocations shown below for adult dogs and weary puppies respectively are based on requirements for beagles, but much larger plaque allocations should be allocated to giant acres such as Saint Bernard's dog or Irish wolf dogs. Therefore, when it comes to other breeds than beagles, the placements must therefore be laid down in consultation with veterinarians and the animal test subject.

The floor space is understood in both tables freely available, as the dogs can move in-that is, the flooring. where fixtures such as shelves and platforms are not obstructing the use of the gold palette-and when calculating the minimum area, any land on the shelves, platforms and the like may not be included.

Table 1. Minimum requirements for the housing of dogs

A sucka-giving bitch and its cutter must have at least the same platoon as a single bitch of equal weight. The food must be so designed as to move away from the pups and to an adjacent area or a swolted platform.

Table 2. Minimum requirements for the termination of wealaced puppies up to 7.5

The above table specifies the minimum floor space, which shall at all times be available for each pup of the weight concerned ; however, there must always be at least four m2 floors of floor space in a plant. Puppies must be off-the-age when they're 8 weeks old.

2.5.4.6. Floor Occupancy

The dogs must be equipped with a solid floor with a smooth, crib-proof stocking. For all dogs, a comfortable and permanent rest area must be provided, for example, by the use of accessories, such as swolled lessings or platforms.

Open floor systems such as gridworks or wireless networks must not be used for dogs.

2.5.4.7. Feeding

The form, content and appearance of the feed shall satisfy the nutritional and behavioural needs of the females ; feed must be bidder and without pollution. For the selection of raw materials, manufacture, preparation and award of the feed, measures must be taken to minimise chemical, physical and microbiological contamination. The feed shall not be used after the expiry date.

Packaging, transport and storage of the feed must be carried out in a manner so that pollution, degradation and destruction of the feed must be avoided. The storeroom must be chilled, dark, dry, and safe from pests and insects. Literally food, such as vegetables, fruit, meat, fish, etc. must be stored in chilling rooms, refrigerators or freezers.

All fodder, treng, and other tools used for feeding shall be cleaned periodically and sterilized if necessary. If wet fodder is used, or if the fodder is easily contaminated with water, urine and osvs, cleaning must be carried out on a daily basis.

Each dog shall have access to the feed and there must be sufficient feeding space to restrict the feeding of the fodder competition. Where necessary, the feed recording must be carried out in order to avoid overweight or malnutrition.

2.5.4.8. Horizontal

There must always be clean drinking water available to all dogs. The supply of water must be established so that the risk of contamination of micro-organisms is minimised.

The water supply facilities must be designed and must be used, so that adequate quality water is provided. There must be sufficient number of beverages to be taken after the number of dogs that have access to them. Where automatic water-supply installations are used, their function shall be controlled, as well as maintaining and periodically rinsed in order to avoid accidents such as the stop or leakage, and the spread of infections. If cages are used with solid bottom, care must be taken to minimise the risk of flooding.

2.5.4.9. It's a cop, man.

Carpets which are close to birth or as a suave shall be provided with a rent and stockings for assistance during childbirth and at the time of the delivery. Puppies shall also be awarded to the necessary degree of power.

2.5.4.10. Cleaning

A very high standard should be maintained for cleanliness and order in housing, washing and storage rooms. Adequate routines for cleaning, washing, decontamination and, where appropriate, sterilization of cages and accessories, bottles and other equipment shall be provided and implemented.

Cleaning and disinfecting systems must be appropriate to the dogs and must not be harmful to their health or welfare.

All experimental animals in which dogs are being barred shall be cleaned at least once a day. All excrement and soiled materials must be removed from all the places where the dogs are disbarred at least once each day and, where necessary, more often.

Cleaning in the form of the guoling of the experimental crops must be carried out when necessary. When the plants are spouted, the dogs must be moved to a dry place so that they don't get wet. They must first be put back when they're pretty dry.

2.5.4.11. Handling

The dogs must, as far as possible, have the possibility of exercise.

The dogs must as far as possible become accustomed to handling during routine operation and during tests.

However, adjustment to handling may be omitted if special circumstances apply, including in the exceptional cases where a dog is trapped in the wild.

The staff needs to talk to, handle, train with, and soigners the dogs as needed.

2.6. Apes

2.6.1. Introduction

This Annex lays down a series of requirements for the installation of apes used or should be used for testing.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the apes is best taken on the basis of the specific conditions.

The Annex shall lay down guidelines for the species which are commonly raised and used for testing. Where there is a need for other species to be used, advice on the needs of these species needs to be sought by persons experts in the animals in question and of animal readers. This must ensure that any inherit needs are taken care of.

2.6.2. Climatic conditions, etc.

2.6.2.1. Ventilation

It shall be provided for adequate ventilation in the cleanup rooms and in the experimental livestock areas so that the needs of the stabled monkeys are fulfilled. The ventilation system must be able to provide adequate fresh air of suitable quality, remove excess heat and humidity, and keep the quantity and spread of smelling, toxic gases, dust and infectious substances of any kind down.

Unless special circumstances apply, or natural ventilation by mechanical ventilation, air shall be carried out 15 to 20 times per. hour in the enclosure room. However, at low levels of stocking, the air shift may be reduced to 8 to 10 times per year. Hour.

It must be ensured that there is no harmful recirculation of untreated air.

The ventilation system must be installed in such a way as to avoid harmful features and noise nuisance.

Smoking is not permitted in rooms where apes are disbarred.

2.6.2.2. Temperature

Silkeapes (Callithix spp.) and tamariner (Saguinus spp.) shall be maintained within a temperature range of between 23 and 28 ° C.

Deadhead apes (Saimiri spp.) shall be kept within a temperature range of between 22 and 26 ° C.

Rhesusaber (M. Moulatta), bear macarchs (M. arclavdes) and marecats (cercopithecus aethiops or Chlorocebus aethiops) must be kept within a temperature range of between 16 and 25 ° C.

Javamakakker (M. fascicularis) must be maintained within a temperature range of between 21 and 28 ° C.

Bavarian (Papio papio and Papio anubis) must be maintained within a temperature range of between 16 and 28 ° C.

When an outdoor installation is used, all animals must be able to get under shelter for bad weather, and they must at any time have access to appropriate, heated indoor systems.

2.6.2.3. Humidity

The relative humidity in the apes for monkeys must be between 40 and 70%.

2.6.2.4. Lighting

Monkeys kept as laboratory animals shall have a 24-hour period of light and 12 hours of darkness. For non-species species such as night apes (Aotus trivirgatus), this cycle may be amended by means of the muted red light in part of the normal working day to enable the observing of the animals during their active periods and to carry out the routine ; pass-through tasks in a secure manner.

Silkeapes (Callithix spp) and tamariner (Saguinus spp.) shall have access to an area of shade within the installation.

Deadhead apes (Saimiri spp. ) who do not have access to the outdoor facilities, shall have access to areas with high intensity corresponding to daylight.

2.6.2.5. Noise

Most high-allied apes have a hearing similar to the human hearing. For these species, a suitable sound level will correspond to what is recommended for the staff.

Certain species such as egernabs (Callitrichidae), silkapes (Callithix spp.) and tamariner (Saguinus spp.) , however, may perceive an ultrasound, which must be taken into account.

Background noise must be kept at a low level and may only exceed 65 DBA in short periods.

Alarm systems that use loud noises are not allowed. Instead, blinking lights can be used that can be seen by staff in every room.

2.6.2.6. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Heat, ventilation, cooling and bemoiling plants and similar installations which are significant for the welfare of the animals must be provided with monitoring devices and alarms to ensure that any possible errors can quickly be registered and remedied immediately.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care must be taken to ensure that the operation of the alarm systems disrupts the animals as little as possible.

2.6.3. Health

An action plan shall be provided by institutions or undertakings to ensure that an appropriate state of health is maintained, which protects the welfare of the apes and meets the scientific requirements ; this action plan must include : microbiological surveillance programme and plans for the handling of major health problems, as well as the definition of health parameters and procedures for the receipt of new apes.

Evidence of the health status of all newcomers must be provided at the cleanup place and the apes must be quarantined upon arrival. During the quarantine period, the health of the animals must be closely monitored and further serological, bacteriological and parasitological tests shall be carried out in accordance with the requirements of competent laboratories.

All monkeys must be under veterinary checks and subjected to periodic diagnostic tests. The staff must also have regular medical screening. If an employee represents a possible health risk for animals, they may not be in contact with them.

hygienic procedures must be laid down to ensure that the potential risk of infection for monkeys and staff to deal with the apes involves minimising the maximum possible level.

A special care must be taken in working with apes, which may be infected with pathogens that can be transmitted to humans. The staff must be informed and measures must be taken to reduce the risk of infection as much as possible. Unexpected morbidity and mortality should be examined in depth, taking into account possible zoonotic diseases.

There must be a health care record for every animal in its entire life.

Apes from different geographical areas must be kept separate from each other until their health status has been clarified.

Deadhead monkeys can be rake-bearers of herpes virus, which, by transfer to silk monkeys, can be lethal. These animal species should therefore be kept in the same unit only if the colonies have been tested free of this virus.

2.6.4. Astining, etc.

2.6.4.1. Restining

A person with knowledge of the behaviour of the apes must be available to provide advice on social conduct, environmental enrichment strategies and operations.

Apes must be in conjunction with one or more species of succesmates to which they can be reconcced.

When a new group is put together, the natural social structure of the species shall be taken into account. In the case of establishment or modification of groups, the animals must be monitored and an action plan must be drawn up on how to manage and minimise aggressiveness.

For group housing, visual barriers must be established which allow animals to stay out of each other's field of vision, as well as escape routes which may prevent attacks and prevent dominant animals from restricting the access of children to parts of the animals ; the facility.

Group setup may only be omitted for veterinary reasons, or if it is required in a trial protocol. Individual culture must be allowed only for as short a time as possible under close supervision, and where it is justified by veterinary or welfare reasons. If individual crops in a small plant are necessary due to a test paradigm, the test manager must justify the duration and scope of the animal ' s stay under limited space conditions, as the likely impact on the welfare of the animal must : be weighed against the scientific value of the experiment. Decisions on the single veterinary clearance must be taken in consultation with the animal technician and the competent person responsible for the welfare of the animals concerned. Where individual crops are authorised, additional resources shall be placed on the welfare of the animals and the care of the animals.

Silk monkeys and tamarines

Silk monkeys and tamarines shall be disbarred in family groups consisting of unrelated couples with a he, a female and one or more cuckolers. Groups of animals not used for breeding shall consist of groups of unequal grown animals or young animals of the same sex that can be reconccated. A precaution must be taken when unrelated adult individuals of the same sex are put together.

In experiments, silkapes and tamarines must be placed together with an animal of the same sex to which they can be reconccated with (twins, parents / children), or parks with a he and a female under the use of contraceptives.

Simplification must be carried out only when necessary by experimental or veterinary reasons. A minimum number of units must be of shortest possible duration and the animals must be able to see all the time, hear and smell of the species, as far as possible.

Silk monkeys and tamarines must first get used to it when they're 8 months old.

Deadhead Monkeys

Deadhead apes must be disbarred into groups of minimum four animals of the same sex. He and dog groups have to be separated to avoid fighting. Particular attention must be paid to identifying distress-led individuals in a group, as aggressive behaviour is not particularly conspinious with the death of the skull of stills.

For breeding purposes the skull of death can be disbarred in groups of between seven and ten females with one or two males. Avlsgroups must have visual contact with other groups, while physical contact between the groups must be prevented.

Deadhead monkeys must first be removed from their families when they are 6 months old.

Mechs and marecats

Mechs and marecats must be kept together with species of species and, as far as possible, in large groups.

For breeding purposes, macka and marecats can be disbarred into groups of between 6-12 females and one he. In the case of the use of linked installations, it must be ensured that in the other part of the installation the male is out of sight in the second part of the facility.

Makaks and marecats must first be removed from their mothers when they are between 8 and 12 months old, except for small children who have been rejected or whose mother does not have enough milk, or if there are other veterinary reasons therefor.

Bavians

Adults and juvenile animals shall be kept together with species of species and shall be kept in groups or pairs of the same sex as far as possible.

For breeding purposes, baboons can be disbarred into groups of one male and 6-7 females or two males and 12-15 females.

Bavians must first be removed from their mothers when they are between 8 and 12 months old, except for small children who have been rejected or whose mother does not have enough milk, or if there are other veterinary reasons therefor.

2.6.4.2. Environmental enrichment

The veterinary reproduction must be designed in a way that allows the apes to carry out a complex programme of activities every day. The animals must have the opportunity to run, go, climb and jump, and materials that stimulate the sense of birth should be available. In order to stimulate the apes, the conditions must be changed regularly.

The environment must make non-human primates in a position to carry out a complex programme of activities every day. The plant must give non-human primates the opportunity to use as wide a behavioural repertoire as possible and to give them a sense of security, as well as a complex environment, that the animals have equal opportunities to run, go, climb and jump.

Silk monkeys and tamarines

Silk monkeys and tamarines must have access to high seating, shelves, swings and tove in nature or artificial material (for example, timber and PVC). Furthermore, there must be a comfortable, safe rest area, such as a set of cases, which can be used for rest, sleep and concealding in fatigatory situations.

Silk monkeys must have access to suitable toys, such as wood slices, with pierced holes filled with rubber arabicum.

Deadhead Monkeys

Deadlines must have access to climbing opportunities, such as walls of the wireless and peat, chains, towing and branches. The animals must also have the seals and the netting areas where they can sit together in a cluster and rest or sleep.

The animals must have different places in the facility where they can be active, where they can retreat from the others and where they can choose pleasant temperatures and light conditions.

Mechs and marecats

Makaks and marecats have advanced cognitive abilities and must be kept in the vicinity of an appropriate complexity, such as fixed-floor installations, which make it possible to conceal spread of feed and encourage fouragation.

Installations for macka and marecats must also include both vertical and horizontal rock-climbing structures which promote the use of the volume of the whole plant. Shelves and seating should not be placed on top of each other. There should be a gap between shelving and the wall of the plant so that the animal can leave his tail to rest.

Mechs and marecats must often have new toys, and the old must be replaced.

Bavians

Bavians have advanced cognitive abilities and must be kept in the vicinity of an appropriate complexity, such as fixed-floor installations, which make it possible to conceal spread of feed and encourage fouragation.

Facilities for baboons shall be robustled and have wide shelves and blocks.

Bavians must frequently have new toys, and the old must be replaced.

2.6.4.3. Dimensions

Apes must be disbarred in installations which allow them to perform a satisfactory register of normal activities.

Indoor the incantation

Indoor stereo must have a height that allows the monkey to escape vertically and sit on a branch or shelf, without its tail touching the floor. There must be a sufficient number of diagonal branches or seating places where all the animals can sit on them at the same time. In the case of wireless on the walls, it must be ensured that the parts of the animals are not liable to hang onto it. Facilities may not be established in two or more layers on top of each other.

Mechs and baboons must be barred in installations with a minimum height that makes it possible for the animals to climb over the human eye level.

Table 1. Minimum requirements for the housing of silkapes and tamarines

Table 2. Minimum requirements for the housing of death-main apes

Table 3. Minimum requirements for the housing of macarchs and marecats

* A facility with minimum sizes can accommodate three animals.

** A facility with minimum sizes can accommodate two animals.

*** For breeding colonies, no additional space / volume for young animals is required for up to two years, which is in place with their mother.

Table 4. Minimum requirements for the intake of baboons

* A facility with minimum sizes can accommodate two animals.

** For breeding colonies, no further seat / volume for young animals will be required for up to two years, which are in place with their mother.

Outdoor installation

As far as possible, Aber must have access to an outdoor installation. Outdoor facilities, however, can only function as a supplement to the indoor litany. Installations made of wood must be made of a type of wood not covered by the animals or being protected by the corded and non-toxic surface treatment. The plant must be partially covered or equipped with leaflet which can protect animals from damp weather and the sun.

2.6.4.4. Feeding

The form, content and appearance of the feed shall satisfy the nutritional and behavioural needs of the apes.

The feed must be bidder and without contamination. For the selection of raw materials, manufacture, preparation and award of the feed, measures must be taken to minimise chemical, physical and microbiological contamination. The feed shall not be used after the expiry date.

Packaging, transport and storage of the feed must be carried out in a manner so that pollution, degradation and destruction of the feed must be avoided. The storeroom must be chilled, dark, dry, and safe from pests and insects. Literally food, such as vegetables, fruit, meat, fish, etc. must be stored in chilling rooms, refrigerators or freezers.

All fodder, treng, and other tools used for feeding shall be cleaned periodically and sterilized if necessary. If wet fodder is used, or if the fodder is easily contaminated with water, urine and osvs, cleaning must be carried out on a daily basis.

All animals must have access to the feed, and there must be sufficient feeding space to restrict the feeding of the fodder competition. Where necessary, the feed recording must be carried out in order to avoid overweight or malnutrition.

The contents of the code and the manner in which it is presented must be varied in order to generate interest and environmental enrichment. The feed shall be disseminated as far as possible, or there must be entire fruits, vegetables or other feedingstuffs that require handling, or to be used as a behavioural animal feed.

The cook must be rich in vitamin C. West monkeys and silk monkeys and tamarines must have adequate amounts of D³'s vitamin, and silkapes, tamarines and skull monkeys must be guaranteed a high protein intake. In addition, the killing of the skull of death females shall also be provided with the appropriate supplements of folic acid.

2.6.4.5. Horizontal

There must always be clean drinking water available to all monkeys. The supply of water must be established so that the risk of contamination of micro-organisms is minimised.

The water supply facilities must be designed and must be used, so that adequate quality water is provided. The number of beverages must be taken care of after the number of animals which have access to them. Where automatic water-supply installations are used, their function shall be controlled, as well as maintaining and periodically rinsed in order to avoid accidents such as the stop or leakage, and the spread of infections. If cages are used with solid bottom, ensure that the risk of flooding is minimised.

2.6.4.6. Substrator, stockings and nesting

In indoor plants, wood chips, wooden granulates with a low level of boot or shredded paper may be used as a sub-layer to track the animals to the fouragation. Grass, plant growth, wood shavings, or barkwood is suitable for outdoor facilities.

Certain monkeys, such as some straw, must be given a form of wood, for example, wool, dry leaves or straw.

2.6.4.7. Cleaning

A very high standard should be maintained for cleanliness and order in housing, washing and storage rooms. Adequate routines for cleaning, washing and decontamination, and, where appropriate, sterilization of cages and accessories, bottles and other equipment shall be provided and implemented.

Cleaning and disinfecting systems must be suitable for the apes and must not be harmful to their health and welfare. There must be clear operational procedures-including registration-for the replacement of stockings in the works.

There must be regular cleaning and, where appropriate, the replacement of the material at the bottom of the installations to prevent it becoming a source of infection and attacks on parasites.

For the cleaning of the facilities, consideration must be given to the behavioural needs of the animals, including the impact of the odourings for social relations, etc. Decision on how often to be cleaned must be taken, taking into account the experimental animal health system, the stocking density and the capacity of the ventilation systems to maintain adequate air quality.

2.6.4.8. Handling mv.

As far as possible, Aber must be trained to cooperate, as this will reduce the stress load that is otherwise caused by handling.

Apes subjected to repeated attempts shall have appropriate recuits periods.

As far as possible, Aber must be transported in harmonious pairs. However, it may be necessary to transport adult animals individually.

2.7. Farm animals and minigries

2.7.1. Introduction

This Annex lays down a series of requirements for the storage of farm animals and minigries used or used for testing. For farm animals, bovine animals, sheep, goats, swine and equidae, including horses, ponies, donkeys and mules shall be considered to be bovine animals. In the case of pigs, a small pig which has been bred to use for experiments and other scientific purposes and has a body weight of not more than 60 kilograms, but of certain tribes, of up to 150 kg. The requirements of this Annex shall apply to both types of pigs, specifying special requirements for minigries, when required.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the animals is best taken on the basis of the specific conditions.

In the case of agricultural research, the animals-where the purpose of research requires animals to be kept under conditions corresponding to the conditions of commercial holdings-at least kept in compliance with the applicable animals ; animal welfare legislation relating to the species concerned.

2.7.2. Climatic conditions, etc.

2.7.2.1. Ventilation

It shall be provided for adequate ventilation in the storage rooms and in the experimental livestock areas so that the needs of the farm animals and the needs of the animals are met. The ventilation system must be able to provide adequate fresh air of suitable quality, remove excess heat and humidity, and keep the quantity and spread of smelling, toxic gases, dust and infectious substances of any kind down. The ventilation system must be installed in such a way as to avoid harmful features and noise nuisance.

For the absence of mechanical ventilation, adequate air quality shall be ensured by means of natural ventilation.

The vacuum level in the air from feed and litter must be reduced as far as possible.

Unless special circumstances apply, or natural ventilation by mechanical ventilation, air shall be carried out 15 to 20 times per. hour in the enclosure room. However, at low levels of stocking, the air shift may be reduced to 8 to 10 times per year. Hour.

It must be ensured that there is no harmful recirculation of untreated air.

The ventilation system must be installed in such a way as to avoid harmful features and noise nuisance.

Smoking is not permitted in rooms where the farm animals and the minigrids are being barred.

2.7.2.2. Temperature

At indoor teams, major fluctuations and sudden changes in the temperature are avoided.

Appropriate temperatures depend on a number of conditions, including race, age, calorific intake, weight, stage of the stage and the environment.

Pigs and minigrims

Pigs and mini pigs kept indoors must be kept in a consistent, temperature-controlled environment in which the whole room is maintained in the termoneutral area. Alternatively, the animals can be kept in a pathway with different microclimates that are established using local heat or two-climate change towards the camp area, as well as with sufficient sock.

Table 1. Indicative temperature range for single-stabled pigs and minigries

In addition to body weight, an appropriate temperature is also dependent on the sexual maturity of whether there are stockings, group litigation and the animal's calorific roof. Animals with a low body weight that do not have access to ironing or there has a limited calorie intake needs temperatures in the high end of the specified intervals.

Newborn piglets must have access to a rental area with a temperature of at least 30 ° C, which may be reduced to 26 ° C when the pigs are two weeks old.

However, in the field of danger and the eastwards, temperatures should not be less than 24 ° C because the supresitive lakes due to a high metabolism tend to tend to heat stress and thus serve as a local heat source.

2.7.2.3. Keep in outdoor inserts

Farm animals kept in outdoors must have access to semi-closures or leaflets to ensure the shelter and shadow of the animals as well as a dry renal area. Semi or leafure shall be placed in the light of these factors and there must be a sufficient number of semi-lean or leafure to protect all animals from bad weather conditions.

Pigs

Pigs held outdoors must have adequate shelter with abundant, dry stocking and additional feed in order to compensate for a lower ambient temperature.

Expendi-equidae

Where kitchens are used, these must be taken off and complied with on a daily basis. Maner and tails provide protection against bad weather and flies, and must not be removed or cut short.

2.7.2.4. Humidity

In controlled environments (climate stalkers), the sudden and powerful fluctuations in humidity are to be avoided.

In the area of indoor teams, the stable buildings must be designed with ventilation, sufficient to prevent the cleanup rooms being moist, and animals become susceptible to infectious diseases.

2.7.2.5. Lighting

All agricultural species must be given enough light, as far as possible must be natural. There must be light for 8 to 12 hours a day, or the natural light cycle should be emulated.

If there are windows, glass that may be broken shall be cut with a protective physical barrier or be outside the scope of the animals.

2.7.2.6. Noise

Iravoidable background noise must be reduced as much as possible, and sudden noises must be avoided. Handling and fixation equipment must be corrected and operated so that the noise can be reduced as far as possible during use.

2.7.2.7. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Heat, ventilation, cooling and bemoiling installations and similar installations which are significant for the welfare of the animals must be provided with monitoring devices and alarms to ensure that any possible errors can quickly be recorded and remedied immediately.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care must be taken to ensure that the operation of the alarm systems disrupts the animals as little as possible.

2.7.3. Health

An action plan shall be provided by institutions or undertakings to ensure that an appropriate state of health is maintained that protects the welfare of the farm animals and meets the scientific requirements ; this plan of action must be implemented ; include a microbiological monitoring programme and plans for the management of major health problems, as well as the definition of health parameters and procedures for the reception of new farm animals.

The person responsible for the test animals shall ensure a regular inspection of the animals and monitoring of their accommodation and care. The supervision must be carried out by a veterinarian or a corresponding competent person.

Health monitoring must be carried out regularly.

hygienic procedures must be laid down to ensure that the potential risk of infection for animals and staff to be handled by the handling of animals is reduced as far as possible.

The necessary measures shall be taken in order to ensure that animals are disposed of with an appropriate health status.

Preventive medical care plans must be developed for all animal species on the basis of veterinary advice, and appropriate vaccination plans shall be used as appropriate.

All animal species must be assured of the need for the need-and clowns, parasitic fighting and nutritional management. Heste must also ensure regular inspection and measures for the prevention of respirations of air traffic.

Care shall be taken to ensure that a lower level of infectious substances or parasites is not conducible or conducits to the growth of infectious substances or parasites.

If abnormal behaviour occurs, such as habitation, earring, biting or chewing on the flank, extract of wool, umbilisation, tissue and reptiles, measures shall be taken immediately to rectify that, including one. assessment of environmental factors and operating practices, etc.,

The horsing of calves or grown-up animals, castration and semi-irperation must be carried out only if it is justified by welfare or veterinary reasons. The performance of these techniques shall be subject to the use of appropriate anaesthesia or anesthesia, unless this will be incompatible with the purpose of the experiment.

Adult sheep and goats of the wool shall be cut at least once a year, unless this will be harmful to their well-being.

Natural raised pigs and applets must first be weaned when they are four weeks old, lamb, the kid and calves of meat bovine animals when they are six weeks old, and animals of the equine family when they are 20 weeks old, unless otherwise justified by veterinary or veterinary health ; welfare reasons.

Animals which are not naturally raised naturally, usually calves of dairy cows, must be fed according to an appropriate plan satisfies nutritional requirements and, as far as ruminants are concerned, promote normal development of the vomit.

Decisions on the former from the mother of welfare reasons or veterinary reasons must be taken in consultation with the animal technician and the competent person responsible for consulting the animals ' well-being.

2.7.4. Astining, etc.

2.7.4.1. Restining

Farm animals must be disbarred into social harmonious groups the size and age of which they are. Farm animals may only be used individually if they are justified by welfare or veterinary reasons.

Decisions on the single veterinary clearance shall be taken in consultation with the animal technician and the competent person responsible for the welfare of the animals concerned. The factors to be taken into consideration include the nature of the animals, their probable response to separation from the group, as well as the need for and the duration of an adjustment period. If individual crops are necessary, the animals must be able to see, hear and smell the species of species.

The setting up of groups must monitor closely the behaviour of all the individuals in the group. When the animals are regrouped, or when a new animal is introduced into a group, it must be ensured that the group will continue to function.

Farm animals shall not be bound unless it is justified by scientific or veterinary reasons, and only in the shortest possible period.

Animals with and without the horns must not be disbarred unless it is a mother and one or more children.

Highly pregnant, focal and newborn animals must be stained in a dry, clean area with a high standard of hygiene, which shall, as far as possible, be made to the extent possible for observation.

2.7.4.2. Environmental enrichment

Farm animals must have access to grazing or to be granted hay, straw or other manipulative objects and rodematerial.

The enriching materials and articles must be changed periodically so that the animals retain interest in these. The committee and the number of enriching objects must be sufficient to reduce aggressive behaviour as much as possible.

Goats shall have access to increased adequate size in a sufficient number to prevent the domination of domination of the animals.

Wine must be given the opportunity to separate functional areas in the paths by either having ample space or the appropriate division of the path. All pigs must, at all times, have access to sufficient quantities of material which they can explore and manipulate, including rumour in, in order to reduce the risk of behavioural disturbances.

2.7.4.3. Dimensions

All animals must have sufficient room to be able to stand, conveniently, stretching and soignate themselves.

Furthermore, the animals must have access to a common area of rental area and adequate space to eat. The camp shall provide all animals the possibility of lying to the lying side at the same time, taking into account whether the species prefers physical contact or spatial separation. At high temperatures, where the animals need to be completely separate in order to facilitate the heat release, the rent shall be greater. The camp shall be stocking with a stocking. Where it is necessary for attempts to fail, the floor shall be designed and isolated in such a way as to improve physical and alert comfort unless an appropriate, controlled environment can be offered.

All installations must be adjusted and maintained so that animals cannot be caught or injured, for example, at separating walls or in feed / rye.

Table 2. Minimum requirements for the housing of bovine animals

Where beds are used for the storage of bovine animals, these boating must be drawn up, taking into account the body size of the animal. The animals must be given the opportunity to make the main movement to the side and vertical and must have adequate parch. The sensing booth must also have a sufficiently soft surface to prevent damage and adequate drainage must be carried out. The height of the step behind must prevent fertilizer from entering into the bonfire, but the step must not be so high that it causes damage to the animals ' laws of entry and exit. The beds of beds must be so wide that the animals can be conveniently down without the pressure on vulnerable parts of the body to be exerting pressure on vulnerable parts of the body. The total number of cutias shall be 5%. higher than the number of animals in order to reduce competition and allow all the animals to lie down at the same time.

Table 3. Minimum requirements for the housing of sheep and goats

*It may be necessary to have higher separatable rooms for adult goats to prevent them from escapes.

Table 4. Minimum requirements for the housing of pigs and minigrims

* Pigs can be kept in smaller installations for short periods, for example by divides the main system with separation walls, provided that it is justified for veterinary reasons, for example when there is a requirement for an individual feed intake.

If the pigs are disbarred individually or in small groups, a more space is required per animals other than by larger groups.

The pigs shall not be bound or kept in vessels or boxes except for short periods during which it is necessary for the purpose of feeding or insemination or for veterinary reasons. The stalling of lakes and piglets must be carried out in a way that allows the soothing to display its special behavioral patterns before and after the experience, as well as the piglets must be given the opportunity to show their natural behaviour after birth. The use of hazards can be used, but the restrictions on the waters around the farm and in the course of the field must be limited as much as possible.

Table 5 Minimum requirements for the housing of animals in the equine family

* However, at least 27 m2.

** In order to ensure adequate space, the minimum requirements for each animal should be based on the test of the animal (s) (stg. ).

The shortest side of the box must be at least 1.5 times the target of the horse, and the height of indoor enclosures shall allow the animals to steep into their full height.

The numbers of the above table are based on the fact that the animals have daily access to additional areas where they can exercise. If this is not the case, the area requirements must be significantly increased.

2.7.4.4. Floor Occupancy

The floors must be made of a material which gives the animals sufficient foothold to allow the unreplicated to move and change position. The floors must be maintained and replaced as required.

In the case of pilescals used for pigs, the camel and the spal dimensions must match the size of the pigs in order to avoid clockings.

No lepts must be used to equidae for the equine family.

2.7.4.5. Feeding

The form, content and appearance of the feed must satisfy the nutritional and behavioural needs of the animals ; additional energy is necessary in the course of the drainage, the adulteration and the growth of the economy.

The feed must be bidder and without contamination. For the selection of raw materials, manufacture, preparation and award of the feed, measures must be taken to minimise chemical, physical and microbiological contamination. The feed shall not be used after the expiry date.

Packaging, transport and storage of the feed must be carried out in a manner so that pollution, degradation and destruction of the feed must be avoided. The storeroom must be chilled, dark and dry. In places where feed is stored, including power fodder, there must be a plan for pest control. Literally food, such as vegetables, fruit, meat, fish, etc. must be stored in chilling rooms, refrigerators or freezers.

All fodder, treng, and other tools used for feeding shall be cleaned periodically and sterilized if necessary. If wet fodder is used, or if the fodder is easily contaminated with water, urine and osvs, cleaning must be carried out on a daily basis.

Each animal must have access to the feed and there must be sufficient feeding space to restrict the feeding of the fodder competition. In the case of secession systems, so much feed must be given in a sufficient number of places that all animals may enter without risk of being harmed.

Where necessary, the feed recording must be carried out in order to avoid overweight or malnutrition.

When feeding on degrazing, the stocking must be checked to ensure that there is enough grass to meet all nutritional needs of the animals and further feed must be allocated on the ground if necessary.

Sudden changes in the broom should be avoided for ruminants and horses, and new fodder must be introduced gradually. Sufficave feed must be provided.

All newborn shall be given a suitable quantity of raw milk as soon as possible after delivery and adequate supplies of raw milk for use in emergencies. Dasminants shall have access to feed from the age of two weeks.

Cattle

The space allocated shall give all animals the chance to eat at the same time, unless the diet is available through libitum. Cattle with horns are required to be more noble than a rhinocerated cattle.

Pigs and minigrims

Minigrise must be given special diet, with low calorific content and high fibrous content to prevent obesity.

In the case of restrictive feeding, young pigs, which are growing, must be fed at least twice a day, while adults are to be fed once a day. All animals in the group must have access to feed without any aggressions occurring. There has to be enough room to make sure that the animals can eat at the same time.

If the animals are the individual or in small groups, the minimum requirement shall be equivalent to the same as in the case of restrictive feeding, cf. Table 6 below. If the animals are kept in larger groups and fed on libitum, they can share aesspace and less place.

Table 6. Minimum requirements for the place of pigs and minigries

* Each animal that feeds on a restrictive basis must have an equitable space that meets at least the objectives stated above.

The Horses

The equidae must, as far as is possible, have constant access to feed in the form of fresh grass, hay, silage or straw. If they do not have the opportunity to graze, they must have an appropriate amount of growfed with long fibres every day. The chicken and hedges shall be designed and placed in such a way that the risk of harm is minimised.

The equidae must be made individually available to the equeys. Where animals cannot be fed on an individual basis, the place must be at least 2,4 metres away and at least one place to be at at least one place. Animals. Horses fed to power fodder must be given small quantities of feed at frequent intervals.

2.7.4.6. Horizontal

All animals must at all times have unrestricted access to fresh, clean water. The number of beverages or the length of the beverages shall be sufficient to enable all individuals within the group. The flow rate of water must satisfy the needs of the individual animals depending on the animal feed, the physiological condition of the animal and the ambient temperature.

If automatic drinking systems are used, they must periodically be checked, supervised and rinsed in order to avoid unintended events.

Cattle

A water trough should be far enough to make it 10%. of the animals may be drinking at the same time. This corresponds to the minimum 0,3 m per head. 10 adult cows. Diegiving dairy cows must have 50%. More space.

For group load in the solution group, at least two drinkers must be at least two drinkers. For groups of over 20 bovine animals, there must be at least one drinking of each 10 head.

Sheep and goats

At indoor storage, there shall be at least one irrigation place per head. Twenty animals.

Pigs and minigrims

For group housing, there must be at least two beverages per a plant or large cup making it possible for several pigs to drink at the same time.

Table 7. Minimum requirements for drinking points for pigs and minigrims

Where pigs in large groups are migrated from an open trough, the circumference of the trenches of the trenches shall be at least a length allowed to allow unhindered access for a single pig (as specified in Table 6 of restrictive feeding), but not less than 12,5 mm length of the length per day. pigs.

Table 8. Minimum requirements for water-drinking water for pigs

The Horses

Horses must have access to an open water surface. If automatic water-nips are used, it must be ensured that the horses are able to use them.

2.7.4.7. Substrator, stockings and nesting

All farm animals must have adequate stockings, and breeding animals must also have adequate outlines or orifitions.

Materials placed on the premises of the animals shall be free of toxin and free of infectious micro-organisms or vermin and other pollutants. Materials on the basis of wood which are chemically treated or containing toxic, natural substances and products that are not clearly defined and standardised may not be used.

The bottom of the plant must constitute a permanent and comfy rest area of all farm animals. All rest areas shall be kept clean and dry.

In the indoor storage of cattle, the length of the area must be large enough for all animals to lay down at the same time. If there is no bedboat, then this area is normally approximately approximately 20 000. 70%. of the minimum area specified in Table 2. The rest of the facility may be without sock and used for feeding and exercise. If the sock area is triggered by individual, open bedside boats, the area's size can be reduced.

2.7.4.8. Cleaning

A very high standard should be maintained for cleanliness and order in housing, washing and storage rooms. Adequate routines for cleaning, washing and decontamination, and, where appropriate, sterilization of cages and accessories, bottles and other equipment shall be provided and implemented.

Cleaning and disinfecting systems must be appropriate to farm animals and minipigs and shall not be harmful to their health or welfare. There must be clear operational procedures-including registration-for the replacement of the material that is the bottom of the plant, so as to avoid it becoming a source of infection and attack by parasites.

In the case of cleaning, consideration must be given to the behavioral needs of the animals, including the impact of the odourings for social relations, etc. Decision on how often to be cleaned must be taken, taking into account the experimental animal health plant, stocking density ; and the capacity of the ventilation systems to maintain adequate air quality.

2.7.4.9. Handling

Animals must be accustomed to human contact, so that fear and psychological burden are reduced as much as possible.

Animals must be confined only in the time necessary for any examination, treatment or sampling, during cleaning of the stables, during milking or loading for transport.

Handling and fixation equipment must be designed in a way that does not cause damage or unnecessary psychological load on the animals.

The animals must be treated calmly and firmly and must not be hunted through passages and corridorways. These should, taking into account the natural behaviour of the animals, must be adapted in such a way as to prevent movement from being prevented and the risk of damage is reduced as much as possible. No physical or electrical side stimuli shall be used.

Scared and suffering must be so wide that two animals are free to pass. However, driver for sheep may only be so wide that there may be one sheep at a time.

In the case of bovine mills, the equipment must be maintained in order to prevent the infectious disease and other diseases.

2.8. Birds (Aves)

2.8.1. Introduction

This Annex lays down a series of requirements for the termination of birds used or used for experiments.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the birds is best taken on the basis of the specific conditions.

In the case of agricultural research, the animals-when the purpose of research requires animals to be kept under conditions corresponding to the conditions of commercial holdings-at least kept in compliance with the applicable animals ; animal welfare legislation relating to the species concerned.

The Annex shall lay down guidelines for the species of bird which are normally used for tests. Where there is a need for other species to be used, advice on the needs of these species needs to be sought by persons experts in the animals in question and of animal readers. This must ensure that any inherit needs are taken care of.

2.8.2. Climatic conditions, etc.

2.8.2.1. Ventilation

The necessary measures must be taken to ensure that birds are not exposed to a move and thus become too cold.

Supporting collections and gases such as carbon dioxide and ammonia must be kept at the lowest possible level.

Smoking is not permitted in rooms where there are disbarred birds.

2.8.2.2. Temperature and humidity

Adult domestic birds, as quail, pigeons, taenses, tamases, tamgeese, guinea fowl and turkeys to be discharged at a temperature of between 15 and 25 ° C and at a relative humidity between 40 and 80%. The interplay between temperature and relative humidity must be taken into account as certain species will suffer during the temperature of the prescribed temperature range if relative humidity is too high.

It may be necessary to have higher volume temperatures than those specified in the table or a local additional heat source, such as a rugelamp for sick or young birds.

Table 1. Guidelines for temperature and relative humidity for domestic tame chickens (G) Gallus domesticus) and tamturkeys (Meleagris gallopavo)

2.8.2.3. Lighting

If birds are kept in artificial light, light must be lit and extinguished gradually, which is particularly important when the bird of birds can fly.

If the birds are given a 24-hour rhythm, this one must not be disturbed.

There must be regular light periods and candlelight that are taken care of after the species.

2.8.2.4. Noise

Birds, such as pigeons that can hear sounds with very low frequencies, must not be disbarred in the vicinal of equipment which emits low-frequency vibrations.

2.8.3. Health

As far as possible, birds must be used that are bred in captivity for tests. If wild birds are used, these must be quarantined and the phantagenation is accustomed before they are used for scientific experiments.

Careful health monitoring and parasitic fighting are particularly important in relation to birds held in outdoorsy systems.

Businesses must ensure that the health of the animals is maintained, have a strategy that protects animal welfare and meets the scientific requirements ; the strategy must include regular health monitoring, a microbiological monitoring programme ; and plans for the handling of health problems and to describe health parameters and procedures for the insertion of new animals.

2.8.4. Astining, etc.

2.8.4.1. Restining

Birds shall be disbarred in installations which contribute to and promote their natural behaviour, including social conduct, exercise and fouragation.

Birds must be disbarred in social harmony groups unless it is impossible because of scientific procedures or welfare requirements, when the birds are regrouped, or when a new bird is introduced into a group, it must be ensured that the group will still be able to function.

The single-use of birds must only take place if it is justified by veterinary or welfare reasons. A single-clearance decision shall be taken in consultation with the animal technician and the competent person responsible for the welfare of the animals concerned.

Tamhens (Gallus gallus domesticus)

Tamhhens shall be disbarred into groups of between five and 20 birds with fewer taps other than harvesters in adult groups, for example in the 1:5 ratio.

Tamkalkunes (Meleagris gallopavo)

Turkeys must be stable in stable groups, which must be monitored in order to avoid harmful feather piling and neck-picking. In accordance with the introduction of veterinary advice, a policy for the handling of litigation must be drawn up.

Overwatch (Coturnix sp, Colinus virginianis, Lophortyx californica, Excalfactoria chinensis)

Careers shall be stamped in voliator or enclosed in groups and exclusively with female or female. If the group includes both sexes, there must be fewer males than females (for example, 1: 4), to reduce aggression between males and the females.

If the quail is discharged in cages, the cages must be merged and added to enrichment elements. Stay in cages must be as short as possible.

Ducks and geese (Anas platyrhynchos, Anser considers domesticus, Cairina moschata)

Ducks and geese must be discharged into groups of suitable size, and birds shall not be alone longer than necessary. In the grace period, it may be necessary to reduce the size of the groups so as to ensure adequate space in the facility.

You're (Columbia Livia)

Dues can be held in groups of mixed sex.

Zebrafinker (Taeniopygia guttata)

Zebras must be kept in groups when they are not used for breeding.

2.8.4.2. Environmental enrichment

Birds must have access to seals and dust and water baths, suitable expots and netting objects, articles to pick up, and the outboard sublayer of the species and individuals who will benefit from this, unless such objects are omitted ; justified by scientific or veterinary reasons.

Tamhens

Gender must have access to an outdoor lion and other suitable hideouts, unless this is not possible for scientific or veterinary reasons.

Where scientific reasons are not used for scientific reasons, a fixed area has to be fixed with a fixed area with a loose sub-layer and object, for example, string bundles, chunblocks, ropes, grazing or halyards.

Egg laying hens shall have access to the nesting boxes at least two weeks before they are ready to lay eggs and no later than 16 weeks old. The single and paropy birds must have access to a nesting box and, by larger groups, the ratio must be at least one nesting box for each two birds. A nesting box has to be closed and big enough for a chicken to turn in it. Wood shavings, straw or another loose layers shall be stranded in the necks to promote exfoliating behaviour. The underlayer must be changed regularly and be kept clean.

Since they are a day old, chickens must always have the opportunity to sit on sticks, hacks in appropriate outlayers, fourakers and take a dust bath in, for example, sand or soft wood shavings / sawdust.

The seating or rods must be 3 or 4 cm in diameter and round with a flat surface. The optimum level of the floorboards varies according to race, age and conditions of installation, but at the beginning of the agony, some must be made about a number of them. 5 to 10 cm above the floor and to the elderly birds 30 cm above the floor. The height of the seater must be adjusted in response to sightings of where easily birds can get up and down from the sticks and move between them. All birds must be able to sit on a stick at the same time, and every grown-up bird has to have a six-inch stick on all levels.

Tamkalkunes

Tamkalkunes must have seating at a height where birds on the ground cannot easily be chopping or dragging in the feathers of the birds. If the birds are older or less agile, access to the agony shall be facilitzed by means of ramps or other equipment, or the torment shall be placed low (for example, 5 cm above). The form and the size of the seater must match the fast-growing claws of the birds. Pindentation shall be the ellipsis of the ellipsis or the rectangular with the rounded corners and the agony shall be made of wood or plastic.

Tamalkalkunes must always have the opportunity to dust in, for example, the fresh sawdust or the sand. If halon bales are used for enrichment and for sanctuary, these frequency shall be replaced.

Careers

Careers need to be allowed to go to hiding places.

Watchers must have access to environmental enrichment in the form of coloured objects such as balls, piping and dice, while grownups must have stones, boil, bolts or branches of trees or bushes to chop in.

Watching must have a sublayer of sand, wood shavings, or straw to fouraging and a place to retreat. In addition, if the fouragation sub-layer is not suited to dust bathing, the birds shall also have access to dust in sand or sawdust.

Eggsby dogs must have access to the nesting boxes and nesting materials, such as hay.

Ducks and geese

Ducks and geese must have access to natural or artificial hiding places, boxes and straw bales.

Ducks and geese shall be kept outdoors or have access to the outdoors of the outdoor unless scientific or veterinary reasons justify indoor disbarrasion. Birds enstabled by outdoor access must be kept safe from predators and must have a dry shelter so that they can rest. They must also have access to vegetation to the appropriate extent to hide in and / or to eat.

The birds must have a tank with a mixture of rock and gravel at the bottom. Drinking machines and basins for water-birds shall be stowed with drainers to the draining of wireless materials to reduce flooding.

Within 24 hours of hatching and in the first day of life, birds must have access to water, such as a flat toast, in order to promote their swim behaviour. Then they must have a flat tank with big stones at the bottom and with feed or gravel scattered among the stones. If there are no parental birds, the young birds must be under surveillance, until it is clear that they are able to leave the water without help and their water-fleshing feathers are beginning to grow. The bassini must be cleaned regularly and the water is replaced as necessary to ensure good water quality.

You're ...

Dues must have an area large enough to fly in, and there must be a separate area with a sitting duck for each bird along the walls of at least one of the plant. Possession must be set up at some time. 30 x 15 centimeters in blocks. The grouns are tied up in the ceiling and stactives can be used instead of seating.

You also need to have access to toys, such as birdbells, mirrors, and hang in chains.

Facilities in which pigeons are kept shall have low-water bird baths. If the pigeons are to be handled frequently, there may be 'nesting areas' or chambers that the birds can be trained to retreat to in order to be caught.

Zebrafinker

Zebra finches need to be staked on the ground floor to promote their natural fourake behavior.

Zebras must be given environmental enrichment in the form of toys, seating and swings. The slots must be set up in various heights to promote normal eating and sleeping behaviour. At least once a week the birds must have hills with water to bathe in with a depth of about 0,5 to 1 cm.

Avlsbirds must have a strap, for example, curves of arrowheads or plastic or wooden crates of dried grass, paper strips or coconut fibres as nesting materials. There must always be access to hiranchor as a nutrition.

If zebra finks are kept in outdoors facilities, they shall have access to shelter and sleep and during cold weather conditions a source of heat shall be set up to the birds.

2.8.4.3. Dimensions

Table 2. Minimum requirements for the housing of domestic guinea fowl

If the minimum requirements for the housing area of scientific grounds are not complied with, domestic hens may be disbarred in smaller cages with appropriate enrichment and a gold area of at least 0,75 m2. Such cages may be used for the termination of two laying hens or small groups of domestic chickens in accordance with the requirements set out in Table 2 for the area as specified in Table 2. Birdie.

The study director shall justify the duration of the animals ' stay under limited space conditions and the decision to that effect must be taken in consultation with the animal technician and the person responsible for advice on the well-being of the animals.

Table 3. Minimum requirements for the housing of the tamkalkunes

All sides of the facility must be at least 1,5 m long.

If the minimum requirements for the housing area of scientific grounds cannot be met, domestic turkeys may be disbarred in smaller cages with appropriate enrichment and a gold area of at least 0,75 m2 and a height of at least 50 cm for birds less than 0,6 kg, 75 cm for birds ; of less than 4 kg (4 lb) of birds over 4 kilograms. Such cages can be used for the storage of small groups of tamkalkunes in accordance with the requirements set out in Table 3 for the area as specified in Table 3. Birdie.

The study director shall justify the duration of the animals ' stay under limited space conditions and the decision to that effect must be taken in consultation with the animal technician and the person responsible for advice on the well-being of the animals.

Table 4. Minimum requirements for the housing of quail

* The ceiling of the Facility must be made from a forgiving material to reduce the risk of head injuries.

Table 5. Minimum requirements for the housing of ducks and geese

* The attachment must include a pool that satisfies the requirements of table 6 below and which may constitute up to 50%. the minimum area of the installation.

** Birds which are already airborne must be kept in installations with a height of at least 75 cm.

Table 6. Minimum required pool size *

* Basic size is per 2 m2 facilities. The base must be up to 50%. the minimum area of the installation.

If the minimum requirements for the housing area of scientific grounds are not complied with, the birds shall be disbarred in smaller cages with appropriate enrichment and a gold area of at least 0,75 m2. Such cages may be used for the storage of small groups of ducks and geese in accordance with the requirements set out in Table 5 for the area as specified in Table 5. Birdie.

The study director shall justify the duration of the animals ' stay under limited space conditions and the decision to that effect must be taken in consultation with the animal technician and the person responsible for advice on the well-being of the animals.

Table 7. Minimum requirements for the termination of pigeons

The anlayons must be long and narrow (for example, 2 m x 1 m) so that birds can take short flight.

Table 8. Minimum requirements for the stalling of zebrafins

The anlayons must be long and narrow (for example, 2 m x 1 m) so that birds can take short flight.

For breeding studies, birds may be barred in smaller cages with appropriate enrichment, a gold area of at least 0,5 m2 and a height of at least 40 cm. The applicant must justify the duration of the birds ' stay under limited space conditions, and the decision thereon shall be taken in consultation with the animal technician and the person responsible for advice on the well-being of the animals.

2.8.4.4. Floor Occupancy

Birds must be stastled on solid floors with layers. However, for research purposes a combination between the ground and wireless floors may be used if the birds have a rest area with a solid floor of at least one third of the floor area of the installation. Thread must be placed under the seaps, if the collection is necessary.

Plastic lambs should be used rather than wireless. If the thread is necessary, the net size must not be greater than the thread to support the base, and the thread shall have rounded edges and be plagued.

2.8.4.5. Feeding

The form, content and appearance of the feed must satisfy the nutritional and behavioral needs of the birds, a part of the diet or additional goods to be spread on the floor of the plant in order to detect to the fourai. The costing can be enriched with fruit, vegetables, seeds, invertebrates, etc. If a new feed is introduced, the previous feed must always be available.

Feedingers must be clearly visible and given in several different places.

Certain species, especially the crusade, must have the brush stone in order to digest their food. The bricks shall be renewed at regular intervals. The birds must also receive a grant of chalice and phospdo in a quantity and in a form appropriate to their life stage.

Feed may be given in forage machines, either attached to the site or at the bottom of the plant. Floor space available to feed machines shall not be available to the birds and shall not be included in the calculation of the land area. Weic-mounted feed machines must be designed and placed so that the birds cannot be trapped under them.

Animals must be tasty and non-contaminated. In the selection of raw materials and in the preparation, preparation and presentation of feed, companies must take measures to minimise chemical, physical and microbiological contamination thereof.

Packaging, transport and storage must be of such quality, avoiding contamination, degradation or destruction. All feed containers and trout and other tools used for feeding shall be cleaned periodically and sterilized if necessary.

Individual animals must be able to have access to the feed and there must be sufficient room to limit competition.

2.8.4.6. Horizontal

Water must be given via drinking cocks, drinker or in a long drinking anal. The number of non-tomatoes or the length of the drinking cup must be sufficient to prevent domineering birds from monopolising water. There must be a drinking or drinking cup for every three or four birds and at least two niples or smallpox in each facility. The feed of birds can also be enriched with additional water.

Animals must always be accessible to unpolluted drinking water.

If automatic drinking systems are used, they must periodically be checked, supervised and rinsed in order to avoid unintended events.

2.8.4.7. Substrator, stockings and nesting

The sublayer shall be absorbent, counteract lesions and have a particle size that reduces the dust as far as possible and prevents the impact of the bird's feet as the most likely. For example, kwood chips, white wood shavings, hathier, washed sand, art grass of plastics or long-lusted rubber seons, but not sandpaper.

The stockings must be kept dry and loose, and the layer of litter must be sufficiently deep to dilute and absorb faeces. An appropriate sublayer in the form of straw or similar to haking after the floorboard shall be spread across the floor.

Fresh hatched chickens and young birds must have a sub-layer that they may be able to hold on to.

2.8.4.8. Cleaning

A very high standard should be maintained for cleanliness and order in housing, washing and storage rooms. Adequate routines for cleaning, washing and decontamination, and, where appropriate, sterilization of cages and accessories, bottles and other equipment shall be provided and implemented.

Cleaning and disinfecting systems must be suitable for birds and may not be harmful to their health or welfare. There must be clear operational procedures-including registration-for the replacement of the material that is the bottom of the plant, so as to avoid it becoming a source of infection and attack by parasites.

In the case of cleaning, account must be taken of the behavioural needs of the birds ; decisions on how often to be cleaned must be taken, taking into account the experimental animal health plant, stocking density and the capacity of the ventilation systems to maintain an appropriate level ; air quality.

2.8.4.9. Handling

There must be suitable equipment for capture and handling, such as well-maintained nets in appropriate sizes and blinded nets with padded edges to small birds.

If regular handling of adult birds is necessary in accordance with the experimental procedure, frequent handling of the chickens is recommended in the event of growth.

In the case of the use of rings as temporary labelling of fast-growing chickens, it is necessary to have regular checks to ensure that the ring does not prevent the growth of the leg.

Labelling methods such as cutting of the feet or sowing at the swimming feet are not allowed.

2.9. Padder (Amphibia)

2.9.1. Introduction

This Annex lays down a series of requirements for the storage of the turtles used or used for experiments.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the paddles is best taken in the best possible way from the specific conditions.

Table 1 indicates the four main habitats of paddles and examples of the species from each habitat which is frequently used for experimental purposes and other research purposes. If there is a need to use species that do not fall within these four habitats categories, advice should be sought to ensure that any special species-specific needs are taken into account.

Table 1. Main categories of habitats and examples of frequently used species broken down by habitat ;

To the extent possible, for tests or other research purposes, paddles which are bred and bred in captivity must be used for tests or other research purposes.

2.9.2. Climatic conditions

2.9.2.1. Ventilation

Installations for padder must have adequate ventilation. Water in installations for the aquarium-timed turtles must be filtered, circulated and illed.

2.9.2.2. Temperature and humidity

Paddles must be kept within the temperature range and under the relative humidity recommended for the species concerned, cf. Table 1 above.

Rum and water temperature must be controlled and paddles must not be subjected to frequent fluctuations in temperature or humidity. Paddles must, as far as possible, have access to areas with different temperature and humidity in order to seek their preferred micromalium.

Paddles that are kept in captivity can be put into hibernation by modifying the rhythm between light and dark and regulating the room temperature if the animals are healthy and in good condition.

Padder must have access to a damp environment, as they absorb fluid through the skin.

2.9.2.3. Lighting

The sound level of the plant must be in accordance with what was expected in the wild and must be used reflecting the natural cycle of the area where the animals are derived.

Both the part of the land living and the living turtles must be able to retreat to areas with a shadow in the plant.

2.9.2.4. Noise

Acotic noise (airborne stimuli) and vibration noise (sublabtive stimuli) must be reduced as much as possible.

2.9.2.5. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Alarm systems must be installed if water-circulation systems are used, and / or if the oxygen is necessary.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care must be taken to ensure that the operation of the alarm systems disrupts the animals as little as possible.

2.9.3. Health

Institutions or undertakings conducting experiments, must draw up an action plan to ensure that an appropriate state of health is maintained, which protects the wellbeing of the paddles and meets the scientific requirements ; include a microbiological monitoring programme and plans for the handling of major health problems, as well as the definition of health parameters and the procedures for the arrival of new turtles.

The person who is responsible for the towers shall ensure that such inspections are regularly monitored, including their storage and care. The supervision must be carried out by a veterinarian or a corresponding competent person. Health monitoring must be carried out regularly.

2.9.4. Astining, etc.

2.9.4.1. Restining

Social behaviors are in the majority of the turtles limited to mating season. Paddles may, however, be disbarred into groups with a view to improving the delivery of food and reducing the freezing of freezing operations.

2.9.4.2. Environmental enrichment

The Paddehabitats of the country must be constructed with, inter alia, branches, leaves, shatters, stones or other suitable materials of art, which allow animals to hide and orient themselves visually and extrorably. The side walls of the terrarium must have a structured surface in order to prevent collision with the glass.

The paddles must have access to hiding places / safe houses, such as a ceramic tube or a plaster. The safe houses must be kept regularly, so that sickness and wounded animals are detected.

Facilities and enrichment elements shall have smooth surfaces and rounded edges in order to minimise the risk of injury to the skin of the amps.

Water turtles

Pamphipons must be kept in base oranges and aquarium. These shall be equipped with a slow flow-flow system for the circulation of non-contaminated (e.g., chlorinated) water, a heat element to maintain adequate temperatures and a pressure-air supply and air for fermentation.

If the installations are not equipped with an appropriate flow-through system, the water shall be replaced by approximately 1. Two times a week.

In installations, the species of Xenopus spp. However, it is enough that the water is replaced regularly to maintain appropriate water quality (e.g. low ammonium levels). The air is not required in these facilities.

Partial aquatic and semi-farming turtles

Partial water living and semi-living toads are to be kept in terrarias with both a rural and a water part. The water part of the terrarium must allow the animals to dive. The water must be changed twice a week, unless a penetratory system is used.

The terrors have to be overcovered. The outside of transparent walls must be overpainted or covered in other ways in order to minimise the damage to the animals. In addition to the layout, the soft scrap material may be used, for example, on the floor near the pool, rocks, pieces of artificial barkmaterial, artificial branches and leaves, and shelves.

The sawdust and other equivalent incidental material must not be used, as it incorpores the sensitive skin of the amps, may contain pathogens and is difficult to clean and reuse.

Pamphibias

The turtles must be given the opportunity to climb and rest. They must also have access to basins, water bowls e.l. where they can diver or seek higher humidity. If water bowls are used, they shall be placed in such a way that the paddles can easily enter and make up of them.

2.9.4.3. Dimensions

Table 2. Minimum requirements for the housing of water living halepadder, e.g. County Wrap spp.

*Measured from snide to tail.

Table 3. Minimum requirements for the housing of water living paddles, for example Xenopus spp. *I

* These recommendations apply to the breeding basins (i.e. stocks), but for reasons of efficiency not for basins used for natural pairing and super ovulation, as these procedures require smaller, individual basin. Minimum requirements apply to adult animals in the specified size categories. Ungals and haleans must either be excluded or a proportionate change must be made to the basic objectives of the bases.

** Measured from snide to sewage opening.

Table 4. Minimum requirements for the housing of semi-water living turtles, e.g. Rana temporaria, for example

*Measured from sniffing to sewage opening.

** 1/3 landdel and 2/3 water part is sufficient to ensure that the animals can dive.

*** Measured from the surface of the ground up to the interior side of the terrarium's top. In addition, the level of the ground must be adapted to its orientation.

Table 5. Minimum requirements for the housing of semi-rural paddles, for example Bufo marinus

*Measured from sniffing to sewage opening.

** 2/3 landdel and 1/3 water part is sufficient to ensure that the animals are able to dive.

*** Measured from the surface of the ground up to the interior side of the terrarium's top. In addition, the level of the ground must be adapted to its orientation.

Table 6. Minimum requirements for the withdrawal of wooden paddles, such as Hyla cinerea

*Measured from sniffing to sewage opening.

** 2/3 landdel and 1/3 of the tank are sufficient for the animals to be able to dive.

*** Measured from the surface of the ground up to the interior side of the terrarium's top. In addition, the height of the ground must be adapted to its orientation, including, inter alia, shelves, large artificial branches and climbing structures.

2.9.4.4. Feeding

Paddles in captivity must have the same diet as they receive in nature, or feed, which is approximated to their natural diet. For example, the living turtles in captivity, for example, can be held on pieces of fish filet or scrapes from frozen liver and heart.

The frequency of foyer shall reflect the climate conditions, including temperature and intensity. Grown-up animals shall not be fed daily, but feeding 1 to 3 times a week to the saturated point of each feeding is recommended.

Animals must be tasty and non-contaminated. In the selection of raw materials and in the preparation, preparation and presentation of feed, companies must take measures to minimise chemical, physical and microbiological contamination thereof.

Packaging, transport and storage must be of such quality, avoiding contamination, degradation or destruction. All feed containers and trout and other tools used for feeding shall be cleaned periodically and sterilized if necessary.

Individual animals must be able to have access to the feed and there must be sufficient room to limit competition.

2.9.4.5. Water quality

The water quality, including the ammonia concentration and water's PH value, must be regularly monitored by water lively and partly water-living amphibilation.

Animals must always be accessible to unpolluted drinking water.

If automatic drinking systems are used, they must periodically be checked, supervised and rinsed in order to avoid unintended events. If cages are used with solid bottom, we must ensure that the risk of flooding is minimised.

It is necessary to ensure that water for use in aquarium and basins is adapted to the needs and tolerances applicable to the individual fish, amphibians and reptiles.

2.9.4.6. Cleaning

The land and water areas of the terrors must be carefully cleaned, so that soil, excrement and food particles are removed.

2.9.4.7. Handling

Paddles must be handled with caution and preferably as little as possible, as their skin can easily hurt.

Chemical tagging of turtles is not permitted, as substances are absorbed through the skin with the risk of poisoning. The cut of the toe must not be made.

In the case of turtles, it must be ensured that the necessary temperature and humidity may be maintained.

2.10. Reptilia of reptiles

2.10.1. Introduction

This Annex lays down a list of requirements for the storage of reptiles used or used for experiments.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the reptiles is best taken on the basis of the specific conditions.

The table below specifies two general habitat categories of reptiles and samples of the species from each habitat which is frequently used for experiments and other research purposes. Where necessary, the use of reptiles which do not fall within these categories must be obtained from experts specialising in the species concerned and from staff to ensure that any special special care is taken ; genetic-specific needs.

Crosscans for experimental or other research purposes must be obtained from reliable suppliers.

Table 1. Two habitats categories and examples of a frequently used reptile species from each habitat ;

2.10.2. Climatic conditions, etc.

2.10.2.1. Ventilation

Installations for reptiles must have adequate ventilation. The venting channel shall be covered with nets to prevent the animals from dotting.

2.10.2.2. Temperature

Reptiles must be kept within the temperature range and under the relative humidity recommended for the kind in question, cf. Table 1 above.

The Rum and water temperatures in the lab must be controlled.

The plants must allow the animals to choose areas with different temperatures so that the animals-which in nature-may choose a micromillet where they can be or may cause heat.

Terrarias for serpents, lizards or lizards from tropical biotopes must have at least one heat element, such as a filament lamp or a heat plate that allows reptiles to increase their core temperature. Heat elements must be thermosta-led to prevent the animals from being overheated and incinerated.

2.10.2.3. Lighting

The appropriate distribution of light and dark times for the species concerned shall be provided for the life cycle of the animals concerned, depending on the life cycle of animals and the seasons.

Crustaceans must be given the opportunity to retreat to shadowing areas of the facility. Light or sunlight should not be the only heat source. ultraviolet must be put in order to stimulate the production of vitamin D.

2.10.2.4. Noise

Acotic noise (airborne stimuli) and vibration noise (sublabtive stimuli) must be reduced as much as possible.

2.10.2.5. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Alarm systems must be installed if water-circulation systems are used, and / or if the oxygen is necessary.

Heating and ventilation systems shall be equipped with monitoring devices and alerts.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care must be taken to ensure that the operation of the alarm systems disrupts the animals as little as possible.

2.10.3. Health

Institutions or undertakings carrying out experiments, must draw up an action plan to ensure that an appropriate state of health is maintained to protect the welfare of the animals and fulfil the scientific requirements ; include a microbiological monitoring programme and plans for the handling of major health problems, as well as the definition of health parameters and the way in which the arrival of new reptiles must be defined.

The person responsible for the test animals shall ensure that the animals are regularly inspecting the reptiles, including their storage and accommodation. The supervision must be carried out by a veterinarian or a corresponding competent person.

Health monitoring must be carried out regularly.

2.10.4. Astining, etc.

2.10.4.1. Restining

Crustaceans must not be disbarred unless it is justified by welfare or veterinary reasons.

Decisions on the single veterinary clearance shall be taken in consultation with the animal technician and the competent person responsible for the welfare of the animals concerned. If individual crops are necessary, it should be limited to the shortest possible period, and the animals must be able to see, hear and smell the species of species.

When the animals are regrouped, or when a new animal is introduced into a group, it must be ensured that the group will continue to function.

2.10.4.2. Environmental enrichment

The habitat of the cross-species must be constructed, including branches, leaves, shatters, stones or other suitable materials of art, which allow animals to hide and orifices visually and extrorably. The cross-line walls in the terrarium must have a structured surface in the form of a pattern to prevent collision with the glass.

Aquatic reptiles must be kept in reservoirs of circulated, filtered and ilted water to be replaced. Two times a week. The water temperature should not exceed 25 ° C in order to reduce bacterial pollution in the water as much as possible. The level of water must be so high that the reptiles can dive completely below the surface of the water.

Water live reptiles must have access to a record as a rest board on which the reptiles can retreat or under which they can seek shelter. The plate must be made of wood or other suitable material so that the animals may have a good grip on their claws when they are to withdraw from the water. The plait needs to be replaced as required.

The living reptiles must be kept in installations with a well-adjusted rural and water part. The water part of the terrarium must allow the animals to dive. The water must be changed at least twice a week, except in the case of flow-through systems. The critters must also have access to appropriate conceal / sanctuaries, such as a clay pipe or other capable of simulating the darkness in an underground lair.

The Terrarios must have close collections and secure the net for all wells, and they must be equipped with a well-seated lid or a lock ending close to and may be closed safely. All lids and lids shall be fitted with paler, hooks or hasper. In some species, all side walls, except for the wall and the top, must be opaque. In the case of irritable or fearsome reptiles, the transparent wall may be covered by a clear cover.

Additional security measures shall be taken where the storage of toxic snakes must be taken.

2.10.4.3. Dimensions, etc.

Table 2. Minimum requirements for the housing of aquatic turtles, for example, Trachemys spp.

* Measured into a straight line from the shield edge to its rear edge.

Table 3. Minimum requirements for the housing of land living snakes, for example, Thamnophis spp.

*Measured from snide to tail.

** Measured from the surface area surface up to the interior side of the terrarium's top. In addition, the height of the ground must be adapted to its orientation, including, for example, shelves and large artificial branches.

2.10.4.4. Feeding

Reptiles in captivity must be kept on the diet that they receive in nature, or on feed or feed, which is approximated to their natural diet. If the animals are fed with dead vertebrates, they must be the life of a humane method which does not result in the risk of poisoning of the reptiles.

The embryodation must be adapted to the nature, the development stage of the animals and the manner in which the animals are kept.

Animals must be tasty and non-contaminated. In the selection of raw materials and in the preparation, preparation and presentation of feed, companies must take measures to minimise chemical, physical and microbiological contamination thereof.

Packaging, transport and storage must be of such quality, avoiding contamination, degradation or destruction. All feed containers and trout and other tools used for feeding shall be cleaned periodically and sterilized if necessary.

Individual animals must be able to have access to the feed and there must be sufficient room to limit competition.

2.10.4.5. Horizontal

All reptiles must have access to drinking water.

Animals must always be accessible to unpolluted drinking water.

If automatic drinking systems are used, they must periodically be checked, supervised and rinsed in order to avoid unintended events. If cages are used with solid bottom, we must ensure that the risk of flooding is minimised.

It is necessary to ensure that water for use in aquarium and basins is adapted to the needs and tolerances applicable to the individual fish, amphibians and reptiles.

2.10.4.6. Substrator, stockings and nesting

It may not be used to use fine sawdust or other demorious materials to be laundered in terrarias, as it may cause serious damage to the mouth, internal injury or intestinal injury, particularly with serpents.

2.10.4.7. Cleaning

A very high standard should be maintained for cleanliness and order in housing, washing and storage rooms. Adequate routines for cleaning, washing and decontamination, and, where appropriate, sterilization of cages and accessories, bottles and other equipment shall be provided and implemented.

Cleaning and disinfecting systems must be appropriate for reptiles and must not be harmful to their health or wellbeing. There must be clear operational procedures-including registration-for the replacement of the material that is the bottom of the plant, so as to avoid it becoming a source of infection and attack by parasites.

In the case of cleaning, consideration must be given to the behavioral needs of the animals, including the impact of the odourings for social relations, etc. Decision on how often to be cleaned must be taken, taking into account the experimental animal health plant, stocking density ; and the capacity of the ventilation systems to maintain adequate air quality.

2.10.4.8. Handling

Crustaceans must be handled with care, since they can easily be harmed. Some lizards, for example, lose their tail if they are handled in an inappropriate manner, and others can easily get traumas.

Clipping of toes is not allowed.

For the transport of reptiles, it must be ensured that the necessary temperature and humidity may be maintained.

2.11. Fish

2.11.1. Introduction

This Annex lays down a series of requirements for the hold of fish used or should be used for tests.

The minimum requirements laid down are minimum requirements to ensure that the welfare of the fish is best taken into account from the specific conditions.

2.11.2. Climatic conditions, etc.

2.11.2.1. Water Transfer

Surveillance systems must be established to ensure that adequate quality of water is given at all times.

The water flow in installations with recirculating systems or filtration must be sufficient to remove the suspension, solid substances and waste substances and ensure that the water quality parameters remain at an acceptable level. The water flow must also be sufficient to ensure that the fish can swim properly and maintain normal behaviour.

In the case of aquaculture, animals must be kept in accordance with the legislation in force relating to fish reared for business purposes.

2.11.2.2. Water quality

The water quality parameters must at all times comply with the acceptable limits for a given species to maintain normal activity and physiology. A journal of relevant water quality parameters must be kept.

A degree of acclimatism in relation to changes in the quality of water may be necessary and must take place above the appropriate period of the appropriate period.

The content of the suspension, fixed substances must be kept within acceptable limits. It must, if necessary, be filtered in order to remove substances which may be harmful to fish and to comply with appropriate parameters for the physical-chemical content of the water.

The oxygen concentration must be appropriate in relation to the relevant species and the surroundings in which they are held. The necessary oxygen concentration varies depending on the temperature, the concentration of carbon dioxide, salty brassignity, forage quantity and the volume of handles. If necessary, the extra oxygen of the water must be provided.

The ammonia of ammonia and nitrite from the excrement of fish must be avoided. This can be done by increasing the flow rate, reducing the density or temperature or by means of biophyping.

Care must be taken to ensure that the water supply systems do not add harmful quantities of carbon dioxide to the plants.

The PH value must be maintained as far as possible, as changes will affect other parameters of the importance of water quality.

Changes to the salt content of water must be gradually introduced.

2.11.2.3. Temperature

Temperatures must be kept within the optimum temperature limits for the species concerned and any changes must be made progressively.

The temperature of the water must be carried out at high temperatures if necessary by the storage facility.

2.11.2.4. Lighting

Fisk needs to have light that mimikes the natural light cycle.

Strong light must be avoided. In the light of the needs of the individual species, the light must be dimpled or the bases covered, and suitable hiding places must be made. Sudden changes in the light conditions must be avoided as far as possible.

2.11.2.5. Noise

The level of noise in experimental units must be kept to a minimum.

Where possible, generators, filtration plants and other equipment that are causing noise or vibrations are not placed in the centre of the fishing plant.

2.11.2.6. Backup and alarm systems

The installation sites which are highly dependent on electrical or mechanical equipment for environmental management and protection of the environment must have a backup system capable of maintaining necessary functions and emergency lighting facilities and ensuring that the alert systems are functioning.

Heat-ventilation, refrigeration and beige installations and similar installations which are significant for the welfare of the animals must be provided with monitoring devices and alarms to ensure that any errors can be registered and remedied immediately.

Clear instructions on emergency procedures must be set up so that they can be clearly visible. Care should be taken to ensure that the operation of the alarm system is disrupts the animals as little as possible.

2.11.3. Health

Fishplants with corresponding pipes must be cleansed and disinfected as necessary ; in closed systems, cleaning and disinfecting must be so carefully maintained that the optimal microbiological conditions are maintained. The network and other equipment must be disinfected each time it has been used. The staff must take the necessary measures to prevent the spread of infection between different fishing pools.

Recently exposed fish-bait must be quarantined during an appropriate period and must, as far as is possible, live separated from existing packs. During the quarantine period, they need to be closely monitored and any illnesses which arise from the disease must be resolved. If not, the stock should be conned.

Raised fish must be acquired from reliable suppliers and must, as far as possible, be the documentation of their state of health.

2.11.4. Astining, etc.

2.11.4.1. Restining

Fisk needs to have enough water to swim normally. The acceptable stock density for a given species varies according to water flow and flow ratio, water quality, the size of fish, health status, age and feeding method.

Precautions must be taken to prevent or minimise aggressive behaviour towards the species.

2.11.4.2. Environmental enrichment

Certain species must have environmental enrichment that takes into account their behaviour patterns. For example, fish must have access to hiding places, while the flatfish must have sand or other layers.

Environmental enrichment must not, however, have a negative impact on water quality.

2.11.4.3. Facilities

Fisk must be kept in land in the appropriate buildings, in the open or in open water.

Access to the installations must, as far as practicable, be controlled and must be positioned in such a way as to allow the fishing to be disturbed at least, and it is easy to maintain adequate environmental conditions.

The materials used for the manufacture of plants on land may not be toxic, but must be durable and must have a smooth internal surface to prevent fish abrasions.

The entries must have a size compatible with the required stock density and shall be able to receive the necessary water flow. The anlayman must have a form that meets the behavioural needs and preferences of fish. The anlayman must be designed in such a way that the fish can not escape. If it is possible, the facilities must be self-purification.

Fish, especially saline fish, can be contained in large liquid plants. The entries must have such a size, including depth, that the fish have the opportunity to swim actively and in hours. The mesh size must allow adequate replacement of the water, but, at the same time, prevent the fish from getting out. The installations must be designed in such a way as to ensure that there is minimal risk of the fish being attacked by predators, so that the form of the plant is not destroyed by tidying or flooding water, thereby catching fish in a trap.

2.11.4.4. Feeding

The form, content and appearance of the feed shall meet the needs of the fishermen.

Fish can be fed with either dried fodder or fresh / frozen animal feed. Since dried fodder affects the water quality, this should be chosen if it can meet the nutritional needs of the species and the fish will eat it.

The fish must be fed with appropriate quantities and intervals, depending on a number of factors such as temperature, size and maturity. The quantity of foyer must be increased by high temperatures which are increasing the metabolished metabolished.

The costing must be presented in a manner that ensures sufficient feed intake. The feeding of fishspawn requires special attention, especially if animal feed is replaced with petfood.

2.11.4.5. Cleaning

All plants must be kept free of the waste products and the surplus feed of fish. The anal must be treated and cleaned regularly to prevent contamination and ensure that the water replacement is not reduced.

Revolting must be avoided in order to prevent contamination of the water in the plant, with the consequent risk of infection. If the installations are not self-purification, waste material must be channelled, as appropriate and preferably as soon as possible, after feeding. The pages and the floor must be regularly cleaned to prevent the accumulation of algae and other forms of dismay. It should, as far as possible, be avoided stressing the fish in connection with cleaning.

2.11.4.6. Handling mv.

Fisk must be dealt with at least.

In normal circumstances, fish must be removed from their normal installations with nets and stunned in a smaller vessel before handling. Fisk must be kept under the drugged as soon as possible and be exposed to clean air saturated water to recover. There must be an effective concentration of anaesthesia throughout the process.

When fishing is to be caught, nets shall be used with a suitable framework and mesh size. The use of the network network is to be avoided. Net must be disinfected and rinsed in cold water before use.

Fish taken out of the water must be handled with wet gloves or wet hands and on a wet surface to prevent their dandruff and slime. The handling method requires special attention in order to avoid dedrying, suffocation or other injury.

Mechanical labelling of fish may not be used unless there is no other means of purpose.

The fish must be labelled with anesthesia in order to facilitate their handling and minimise the risk of injury, morbidity and stress.

Fish shall not be fed for a period prior to transport. Measures must be taken to prevent fish from getting hurt and stress when they are caught, transported and unloaded.

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Appendix 3

Methods for the killing of animals

1) For the killing of animals, the methods indicated in the table below (Table 3) shall be used in accordance with the requirements of the table.

Other methods other than those listed in the table may be used :

a) on animals who are unconscious and do not regain consciousness before they die ;

b) in the case of animals used in the case of agricultural research, when the purpose of the project requires that the animals are kept under conditions corresponding to the conditions of commercial holdings ; these animals may be killed in accordance with the requirements of : Annex I to Council Regulation (EC) No, 1099/2009 of 24. September 2009 on the protection of animals at the time of killing.

2) The killing of animals must be brought to an end by one of the following methods :

a) confirmation that the circulation has been permanently discharged, 1)

b) the destruction of the brain,

c) the dislocation of the throat,

d) bleeding ; or

(e) Confirmation of rigor mortis set in. 2)

After completion of livitation, each animal is checked and checked that each animal is dead. Checks shall be carried out by confirmation that the circulation has been discharged permanently or by confirmation that rigor mortis has entered, cf. point 2 (a) and (e).

Methods of treatment which do not immediately lead to the death of the animal must be completed as quickly as possible and before the animal is completed with the killing of the animal at the appropriate method of killing for the animal species in accordance with the animal species. this Annex, and with ex post verification that the animal is dead.

Table 1. Methods of treatment (*) and the killing methods

*Bemoring methods

Claim

(1) In the case of use, where appropriate, after prior assessment.

(2) The use of large reptiles is used.

(3) The only way to apply the space in which the animals are met is gradually being achieved. is not used on embryos of rodents and newborn rodents.

(4) only used on birds of less than 1 kg. Birds of more than 250 g of seisers.

(5) Only on rodents can be used under 1 kg. Rodents on over 150 g's seers.

(6) only applies to rabbits less than 1 kg. Rabbits of over 150 g Seers.

(7) only applies to birds of less than 5 kg.

(8) only applies to rodents less than 1 kg.

(9) only applies to rabbits less than 5 kg.

(10) The use of newborn babies is only used.

(11) The use of birds in less than 250 g shall be used.

(12) Only use if it is not possible to use other methods.

Requires special equipment.

(14) The use of pigs only.

(15) are used only by experienced shooters in the field.

(16) Only use of experienced marksmen in the field if it is not possible to use other methods

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Appendix 4

Classification of Attempting for Workload

4.1. Categories of Workload Rate

Terminal :

Attempts carried out exclusively under the universal anaesthesia and then the animal will not regain consciousness shall be classified as "terminal".

Light Workloan :

Attempts to animals which involve animals likely to experience short-term pain, suffering or anxiety, and tests without any significant weakening of the well-being or general state of the animals are classified as "lightload incriminating".

Moderate Workloinating :

An attempt on animals likely to experience short-term moderating pain, suffering or distress or prolonged incriminating pain, suffering or distress, and attempts likely to cause moderate deterioration in the animals ' s animals ; well-being or general conditions are classified as "moderately condencting".

Convivient stress :

Experiment on animals which mean that the animals are likely to experience significant agony, suffering or distress or prolonged moderating pain, suffering or distress, and attempts likely to cause significant deterioration in the wellbeing of the animals or general conditions are classified as "significant incriminating".

4.2. Ratings criteria

Where the severity level is to be categorized, account shall be taken of any procedure or any handling of an animal within a defined study. It is determined from the most damaging effects which each animal is expected to experience after all appropriate fins of finals have been used.

For the execution of an attempt to a particular category, account shall be taken of the type of test and a number of other factors. All these factors will be assessed in every case.

The factors relating to the study shall include :

-WHAT? the type of handling,

-WHAT? the nature of pain, suffering, distress or permanent damage caused by (all elements of the test and its) ;

-WHAT? the intensity, duration, frequency and the different techniques used,

-WHAT? incubatory condition during an experiment,

-WHAT? prevent the expression of natural behaviour, including restrictions on the standards of housing, care and care ;

In section 4.3, examples of tests that are classified with one of the aforementioned loading levels are given on the basis of factors relating to the type of test themselves. They provide a first indication of the classification that will be the most appropriate for a given type of test.

However, for the purpose of the final determination of the burden of an experiment, the following additional factors shall also be taken into account, which shall be assessed in each case :

-WHAT? Type and genotype ;

-WHAT? the maturity, age and sex of the animal ;

-WHAT? the training of the animal in relation to the study,

-WHAT? if the animal is to be re-used, the incrimination of the previous attempts,

-WHAT? the methods used to reduce or eliminate pain, suffering and anxiety, including the refining of the housing, care and care facilities,

-WHAT? humane traits.

4.3. Workload degrees

Examples of different types of test, which are classified with one of the load graders on the basis of factors related to the type of test themselves :

1. Light burdenshing :

a) Practice, except for the sole purpose of being alive.

b) Pharmaco-kinetic enquiry, where a single dose is administered and a limited number of blood samples (in total < 10% of blood volume) and is not expected to cause any traceable adverse effect.

c) Noninvasive imaging (i.e. MRI) with appropriate sedation or anaesthesia.

d) Survical trials, i.e. the ear and halebiopsy, non-surgical subcutaneous implantation of minipumps and transponders.

(e) The use of remote telemetry devices that only cause less deterioration in animals or less disturbance of normal activity and behaviour.

(f) Subcutaneous, intra-muscular, intraperitoneal ways of administration, via probe and intravenous via superficial blood vessels, where the substance only has an easy impact on the animal, and the quantity and nature of the animal is appropriate.

g) Insertion of tumours or spontaneous tumors that do not cause unequal clinical damage (i.e. small, subcutaneous, non-invasive knots).

(h) Respiratory of genetically modified animals expected to result in a faecal type with an easy effect.

i) The feeding of diet which does not satisfy all the nutritional needs of all animal nutrition and is expected to cause an easy clinical abnormality within the period of the investigation.

j) Short-term holding (< 24 hours) in mesh recovery times.

c) Studies involving short-term deduction of social partners, short-term housing of adult rats or mice of social strains are in solitude.

I) Models exposing animals to harmful stimuli that are succably to be associated with light pain, suffering or anxiety, and which animals may well avoid.

m) A combination or accumulation of the following examples may lead to classification as easily condensing :

i the assessment of body-building with non-invasive measures and a minimum size of restrictions ;

ii monitoring by means of an ECG with non-invasive techniques, with a minimum or no restriction on the animals of the animal ;

iii the use of remote telemetry devices which are not expected to cause a weakening of socially adapted animals and not disrupters normal activity and behaviour ;

iv in the case of genetically modified animals, which are not expected to have any clinical portable harmful phenotype ;

v addition of idle marklet in the broom to follow the passage of the abdominal holding,

We removal of feed in < 24 hours for adult rats ;

vii open field test.

4.3.1. Moderate Workloinating :

a) Frequent use of test substances which produce moderate clinical effects and the taking of blood samples (> 10% of the blood volume) of a conscious animal within a few days without the volume replacement.

b) Acutte dose-range finding studies, chronic toxicity / carcinogenicity test with non-lethal end points.

c) Surgery under the universal anaesthetic and appropriate anesthesia in pain, suffering, or weakening of the general state of operation. For example : thoracotomy, craniotomi, laparotomi, orchiectomy, lymphadenectomy, thyroid cectomy, orchionectomy, with effective stabilization and vulnerability, organ transplantation, with effective control of mold, catheters or biomedical equipment (e.g. telemetry transmitters, mini pumps, etc).

d) Models for the pasting of tumours or spontaneous tumors that are expected to cause moderate agony or anxiety or moderate interference with normal behaviour.

(e) Radiation or chemotherapy with a subletal dose or by an otherwise available dose, but with the reconstruction of the immune system. Harmful effects are expected to be easy or moderate and short-lasting (< 5 days).

(f) Respiratory of genetically modified animals, which is expected to result in a phenotype with a moderate effect.

g) The creation of genetically modified animals through surgical trials.

(h) The use of mesh cages resulting in moderation of freedom of movement in a long-term period (up to 5 days).

i) Studies with changes in diet which do not satisfy all animal nutrition requirements and are expected to cause moderate clinical abnormality within the period of the examination.

j) Removal of feed in 48 hours for adult rats.

c) Invocation of escape and evasion reactions where the animal is not able to escape or evasive stimulus, which is expected to result in moderate anxiety.

4.3.2. Convivient stress :

a) Toxicity test 1) is likely to be expected and there are severe pathologic conditions. For example, test for acute toxicity in a single dose (see The OECD guidelines for testing).

b) Testing of equipment where failure can cause significant pain, fear or death 2) for the animal (e.g. cardiac machines).

c) Testing of the strength of vaccines characterized by permanent weakening of the condition of the animal, the forwarding disease leading to death 3) , connected with long-term moderate pain, fear or suffering.

d) Radiation or chemotherapy with a lethal 4) the dose without the reconstruction of the immune system or the reconstruction of the production of graft versus host disease.

(e) Models for the pasting of tumours or spontaneous tumours which are expected to cause a deadly disease to be caused by prolonged moderate agony, fear or suffering. For example, the tumors that induction cakeksi, invasive bone tumors, tumors that lead to metastatic dispersion.

(f) Surgical and other measures in animals under the universal anaesthesia expected to result in significant incriminating or persistent moderate suffering, suffering or distress after the operation, or significant incriminating and persistent weakening of the animals ; general mode. Calciation of unstable fractures, thoracotomi, without appropriate analgetics or trauma inducing multiple organ failure.

g) Organ transplantation, where organ donation is likely to lead to significant incriminating anxiety or the weakening of the general condition of the animals (e.g. xenotransplantation).

(h) Sport of animals with genetic disorders expected to experience significant and persistent weakening of the general state, such as Huntington's chorea, dystrophiphia musculorum progressiva, chronic recidivergence neuritis models.

i) The use of mesh cages, which results in considerable strain restrictions on the freedom of movement in a long-term period, are used.

j) Inevitably electric shock.

c) Completely isolation in long-term social species, for example, dogs and non-human primates.

I) Immobilisation stress to induce abdominal abdominal arrhythm or heart disease.

1) The commotion contains provisions implementing the European Parliament and Council Directive 2010 /63/EC of 22. September 2010 on the protection of animals used for scientific purposes, the EU-tenth 2010, nr. L 276, s. 33. The notice also contains provisions that implement parts of Directive 2006 /123/EC of the European Parliament and of the Council of 12. In December 2006 on services in the internal market, EU-tiering 2006, no. L 376, s. 36.

1) Point (a) is not a method of killing, but a method for finding that death has been met.

2) Point (e) is not a method of killing, but a method for finding that death has been met.

1) As a starting point, death must not be a final point for the trials.

2) As a starting point, death must not be a final point for the trials.

3) As a starting point, death must not be a final point for the trials.

4) As a starting point, death must not be a final point for the trials.