64/2015 Sb.
DECREE
of 31 March 2004. March 2015,
amending Decree No. 380/2003 Coll., on animal health requirements for
trade in semen, the egg cells and embryos and on animal health
the terms of their imports from third countries, as amended
The Ministry of agriculture lays down pursuant to § 78 of Act No. 167/1999 Coll., on the
veterinary care and amending certain related laws (health
Act), as amended by Act No. 131/2003 Coll., Act No. 316/2004 Coll., Act
No 48/2006 Coll., Act No. 182/2008 Coll., Act No. 227/2009 Coll., Act
No 308/2007 Coll., Act No. 359/2009 Coll., Act No. 279/2013 Coll., Act
No 139/2014 Coll. and Act No 264/2014 Coll., to implement section 28 para. 5, §
31 para. 3 (b). and § 34 paragraph 1). 5 of the Act:
Article. (I)
Decree No. 380/2003 Coll., on animal health requirements for trade in
semen, egg cells and embryos and health conditions
their imports from third countries, as amended by Decree No. 155/2006 Coll.
Decree No. 447/2009 Coll. and Decree No. 125/2013 Coll., is amended as follows:
1. in article 15, paragraph 2. 1 (b). (b)), the word "community" shall be replaced by
"The European Union".
2. in annex No 9 of part B, section I, paragraph 1. 5 (b). (b)),
"infectious equine Arteritis" the words "or on the findings of its
the genome of the chain reaction employed or employed a chain
the reactions carried out in real time ".
3. in annex No 9 of part B, section I, paragraph 1. 5 (c)):
"(c) the test to determine the originator) contagious equine metritis carried out with
negative result on samples taken 3 be stallions
twice at an interval of not less than 7 days and not earlier than 7 days for
systemic treatment or 21 days for treatment after possible antimicrobial
the treatment of the donor Stallion at least from the foreskin, the urethra and the fossa
glandis.
1. Before sending it to the lab samples must be placed in the
transport medium with activated carbon, such as Amies.
2. the samples shall be subjected to at least one of the following tests:
2.1 culture for mikroaerofilních conditions for at least 7 days to
isolation of Taylorella equigenitalis, based within 24 hours after you remove the
a sample of the donor animal, or 48 hours in cases where
the samples during transportation, refrigerated, or
2.2 polymerase chain reaction or polymerase chain reaction
real time detection of Taylorella equigenitalis on genome, made to
48 hours of removing samples from the donor animal. ".
4. in annex No 9 of part B, section I, paragraph 1. 6 (a). and) after the word
"the stallions", the words "at least once a year at the beginning of the breeding
season or "after the words" semen collection "with the words" intended for
"and at the end of the text, the words" before the first collection
semen ".
5. in annex No 9 of part B, section I, paragraph 1. 6 (b)):
"(b)) if the donor Stallion was continuously positioned at the Centre for
the collection of semen for at least 30 days prior to the first semen collection and after
for a subscription, but can occasionally leave the station with a written
the consent of the veterinarian Centre on a 24-hour period of less
than 14 days or other equidae on the collection of semen
come into direct contact with equidae of lower health status,
the tests shall be carried out in accordance with paragraph 5 as follows:
1. at least once a year on samples taken to be stallions
the beginning of the breeding season or prior to the first semen collection intended for the
trade and at least 14 days after your initial date of location of the continuous
at least 30 days prior to the first semen collection,
2. during the period of semen collection intended for trade as follows:
2.1. the test referred to in paragraph 5 (b). and on samples taken up) 90
days prior to semen collection intended for trade,
2.2. the test referred to in paragraph 5 (b). (b)) on samples taken within 30
days prior to semen collection intended for trade, except in the case where the test
virus isolation, polymerase chain reaction or polymerázová
chain reaction in real time made on samples of aliquots
all semen collected a maximum of 6 months before semen collection
intended for the trade confirms that the donor Stallion is not a promoter, and when
the donor Stallion reacted positively in the serum neutralization test on
infectious Arteritis horse serum diluted at a ratio of at least 1 in 4,
2.3. the test referred to in paragraph 5 (b). (c)) on samples collected up to 60
days prior to semen collection intended for trade, which may be the case
the polymerase chain reaction or polymerase chain reaction
real time performed on 3 samples (smears) taken only once; ".
6. in annex No 9 of part B, section I, paragraph 1. 6 (a). (c)), in the introductory part of
provisions, the word "or" is replaced by "and" and in the final part of the
provisions, the words "that must be carried out twice a year, is confirmed by the
as seropositive for infectious Arteritis in horses Stallion "are replaced by
the words "polymerase chain reaction or polymerase chain reaction
real time made on aliquots of all semen collected
at least twice a year at a four-month interval, confirms that the
conveyor seropositive Stallion is not, and when the donor Stallion responded
positively in the serum neutralization test for equine viral Arteritis with
serum diluted at a ratio of at least 1:4 ".
7. In Annex 9, part C, section II, paragraph 1. 1 (b). (j)), the word
"40" is replaced by "50".
8. in annex No 9 of part (D) of paragraph 1. 4 (b)), and (c)):
"(b)) must have a negative result on the immuno-diffusion test for Cogginsova
agar gel or an ELISA test for infectious anaemia,
horses carried out on a blood sample taken not less than 14 days after the
the start date of the placement lasting at least 30 days under subparagraph (a)), and
no more than 90 days prior to the collection of ova or embryos intended for trade,
c) must be subjected to the test for determining the causative agent of contagious equine metritis
carried out with negative results in the laboratory under part B, part I.
paragraph 5 on at least two samples obtained from the donor Mare
first 7 days for systemic treatment or 21 days for treatment after possible
antimicrobial treatment of the donor Mare from at least slizničního the surface
Fossa clitoralis and sinus clitoralis.
1. the samples must be taken in the period referred to in subparagraph (a)) twice in succession
at an interval of at least 7 days for the tests referred to in point 3.1 and once for the tests
According to point 3.2.
2. Before sending it to the lab samples must be placed in the
transport medium with activated carbon, such as Amies.
3. the samples shall be subjected to at least one of the following tests:
3.1 conditions for cultivation mikroaerofilních for at least 7 days to
isolation of Taylorella equigenitalis, based within 24 hours after you remove the
a sample of the donor animal, or 48 hours in cases where
samples kept refrigerated during transportation; or
3.2 the polymerase chain reaction or polymerase chain reaction
real time detection of Taylorella equigenitalis on genome, made to
48 hours of removing samples from the donor animal. ".
Article. (II)
The effectiveness of the
This Decree shall enter into force on 1 January 2000. may 2015.
Minister:
Ing. Abdoul in r.
