125/Sb.
DECREE
of 17 May. may 2013,
amending Decree No. 380/2003 Coll., on animal health requirements for
trade in semen, the egg cells and embryos and on animal health
the terms of their imports from third countries, as amended
The Ministry of agriculture lays down pursuant to § 78 of Act No. 167/1999 Coll., on the
veterinary care and amending certain related laws (health
Act), as amended by Act No. 131/2003 Coll., Act No. 316/2004 Coll., Act
No 48/2006 Coll., Act No. 182/2008 Coll., Act No. 227/2009 Coll., Act
No 308/2007 Coll. and Act No. 359/2012 Coll., to implement section 31 para. 3
(a). and § 34 paragraph 1). 5 of the Act:
Article. (I)
Decree No. 380/2003 Coll., on animal health requirements for trade in
semen, egg cells and embryos and health conditions
their imports from third countries, as amended by Decree No. 155/2006 Coll.
Decree No. 447/2009 Coll., is amended as follows:
1. In paragraph 1, the term "edits in accordance with the law of the European
Community ^ 1) "is replaced by" incorporates the relevant provisions
The European Union ^ 1) and edits ".
2. In paragraph 2, at the end of the letter o) dot is replaced by a comma and the following
letter p) is added:
"p) Centre for the storage of semen-approved and monitored
a business or other equipment on the territory of a Member State or a third country in
which is stored semen of the ovine, caprine or equine species. ".
3. In article 3, paragraph 3. 1 (b). (b)), section 6 (a). (b)) and in section 9 (a). a) and b), the words
"health status" shall be replaced by the words "health status".
4. In article 3, paragraph 3. 1 (b). (d)), the words "on a form issued by the State
the veterinary services in accordance with the model laid down for the
these cases by the Commission of the European Community (hereinafter referred to as "form
corresponding to the specified pattern ")" shall be replaced by the words "in accordance with the model
laid down by the legislation of the European Union ".
5. In section 4, paragraph 4. 2, the words "Commission of the European Community ' shall be replaced by
the words "European Commission".
6. In section 4, paragraph 4. 2 and 3, § 7 (2). 2 and 3, section 10, paragraph 1. 2 and 3, § 13 para. 2 and
3, § 24 para. 1 and in paragraph 25, the words "the State Veterinary Administration" shall be replaced by
the words "Central Veterinary Administration".
7. In paragraph 6 (b). (d)), section 9 (b). (d)), section 12 (a). (d)) and § 14 para. 1 (b).
(d)), the words "on a form conforming to the model laid down" shall be replaced by
the words "in accordance with the model laid down by the legislation of the European Union".
8. In paragraph 12 (a). and), after the words "semen collection centre ' shall be
the words "or semen storage centre".
9. In § 13 para. 1, after the words "semen collection centre ' shall be
the words "and semen storage centres".
10. In § 13 para. 2, after the words "semen collection centre ' shall be
the words "or semen storage centres".
11. In § 13 para. 3, after the words "semen collection centres"
the words "and semen storage centres".
12. in § 14 para. 1 at the end of the text of subparagraph (a)) the following words "or
produced by the approved embryo production team; the conditions for the
approval of embryo collection team and production team for embryos are listed
in part A of Annex 9 to this Decree ".
13. in article 15, paragraph 2. 1 (b). and), the words "the territory of the Member States (hereinafter referred to as
"territory of the community") "shall be replaced by" the territory of the Member States (hereinafter referred to as
"the territory of the European Union") ".
14. in article 15, paragraph 2. 1 (b). (b)) § 16 para. 1 (b). a) and b), § 17 para. 1
(a). and, § 19 paragraph 1). 1 (b). and) and in § 23 para. 2,
"The community" shall be replaced by the words "European Union".
15. in article 15, paragraph 2. 2 (a). (c)), § 16 para. 2 (a). (c)), § 17 para. 2 (a).
(c)) and in section 19 para. 1 (b). (c)), the words ", on a form corresponding to
the well established pattern "shall be replaced by the words" in accordance with the model laid down by the
regulations of the European Union ".
16. in section 18 para. 2, the words "annex 1" shall be replaced by ' in annex
No. 7 ".
17. in § 19 para. 2, the words "their collection and embryo collection"
replaced by the words "semen collection centres or semen storage centres,
embryo collection or production teams "teams.
18. in paragraph 23 of the paragraph. 2 and in section 27 para. 2, the words "European Community",
replaced by the words "European Union".
19. section 26 is repealed.
20. In section 27 para. 2 the words "in accordance with § 48 para. 1 (b). about point 2)
the Act "shall be deleted.
21. in annex 1, section B (b). (f)) (i)) of the second and third indents,
Annex 2, section B, point 3, annex 3, paragraph 3 and annex No. 8 point
1 (b). and, ' community) "shall be replaced by" European Union ".
22. in annex 1, section B (b). (f)) at the end of sub-item iii text)
the words "with the exception of single-use instruments".
23. In annex 1, section B (b). (f)) at the end of the text below)
the words "except for single-use".
24. in annex 1, section B (b). f) subsection viii), the words
"semen collection centres, which are contained in paragraph 2" shall be replaced by
"banks of the semen".
25. in annex 2, section A, para. 1 (b). (d)) the introductory part of the provisions of the
the word "examination" shall be replaced by the word "examination".
26. in annex No. 3 name: "the ANIMAL HEALTH REQUIREMENTS for BOVINE SEMEN
FOR TRADING WITHIN THE EUROPEAN UNION OR IMPORTED INTO THE EUROPEAN
The UNION ".
27. Annex No 5 and 6 are added:
' Annex No. 5 to Ordinance No. 380/2003 Coll.
ANIMAL HEALTH REQUIREMENTS FOR SWINE TO BE TAKEN TO CENTRES FOR THE COLLECTION OF
SPREMATU OF PIGS AND THEIR INVESTIGATION
And
Requirements for pigs to be taken into semen collection centres
(1) all the animals taken into semen collection centres must:
and) be subjected to isolation of at least 30 days in the device that was
approved for this purpose in particular, the regional veterinary services and
which are present only animals of at least the same animal health
status;
(b)), before entering into isolation referred to in subparagraph (a)) chosen from herds
or the economy,
1. which are free of brucellosis in accordance with chapter on brucellosis in pigs
Terrestrial animal health code of the World Organisation for health
animal health (OIE),
2. in which during the preceding 12 months, there was no animal
vaccinated against foot-and-mouth disease,
3. in which has not been detected in the preceding 12 months no
clinical, serological, virological or pathological evidence of Aujeszky's
disease,
4. that are not located in a restriction zone defined by the Decree
No 299/2003 Coll., Act No. 389/2004 Coll. or Decree No. 202/2004 Sb.
due to the presence of the disease in pigs.
The animals may not previously have been kept in any herd of a lower health
status than the status described in points 1 to 4;
c) during the 30 days prior to entering the insulation specified under subparagraph (a). and)
experience with negative results the following tests, carried out in
accordance with the standards set out or referred to in the relevant legal
the regulations of the European Union:
1. as regards brucellosis, the tests for brucellosis with buffered
brucelovým Antigen (rose Bengal test), or the cELISA or
iELISA,
2. as regards Aujeszky's disease
2.1. in the case of non-vaccinated animals testing ELISA to detect
antibodies to the whole Aujeszky's disease virus or its glycoprotein B
(ADV-gB) or glycoprotein D (ADV-gD) or a serum neutralisation
the examination,
2.2. in the case of animals vaccinated gE GI deleted vaccine,
ELISA tests for detection of antibodies to glycoprotein E (ADV-gE) virus
Aujeszky's disease;
3. as regards classical swine fever ELISA tests for antibodies or
a serum neutralisation tests.
Respond to some animal brucellosis tests referred to in point 1
positively, not to be animals which have reacted negatively, from the same
economy taken into isolation until the status of the herd has been confirmed
or the holding of origin the animals with positive results as plain
brucellosis.
The regional Veterinary Administration may allow them to be carried out in isolation
tests covered in this paragraph refers, when results are known
before the start of insulation specified in subparagraph (a). and).
As regards Aujeszky's disease serological tests carried out in the
accordance with the provisions of this Decree shall comply with the standards laid down in annex No. 3
Decree No 382/2003 Coll.;
(d)) to be subjected to the following tests carried out on samples
taken during the last 15 days laid down in (a) isolation. and):
1. as regards brucellosis, the tests for brucellosis with buffered
brucelovým Antigen (rose Bengal test), or the cELISA or
iELISA;
2. as regards Aujeszky's disease
2.1. in the case of non-vaccinated animals testing ELISA to detect
antibodies to the whole Aujeszky's disease virus or its glycoprotein B
(ADV-gB) or glycoprotein D (ADV-gD) or a serum neutralisation
the examination,
2.2. in the case of animals vaccinated gE GI deleted vaccine,
ELISA tests for detection of antibodies to glycoprotein E (ADV-gE) virus
Aujeszky's disease.
Responds to some animal brucellosis tests referred to in point 1
positive and if the suspicion of brucellosis is excluded in accordance with the
(a). (e)), it must be immediately removed from the isolation of the animal.
Responds to some animal tests for Aujeszky's disease referred to in
point 2, this animal must be immediately removed from isolation.
In the case of group isolation, the animals must regional animal health management
take all necessary measures to ensure that the remaining
the animals in the examinations referred to in paragraphs 1 and 2 has responded
negatively, they have before they are admitted to the semen collection centre
in accordance with this annex to Decree, satisfactory health status;
e) the measures taken in the case of suspicion of brucellosis:
As regards animals which react when tests for brucellosis that
in subparagraph (a). (d)), point 1, the following procedure applies:
1. the positive sera are subjected to at least one of the alternative tests
referred to in point (a). (d)), point 1, which was not carried out on the samples
referred to in point (a). (d));
2. on the holding of origin the animals shall be carried out in response,
the epidemiological enquiry;
3. in the case of animals referred to in point (b) during the examinations. d) point 1 and in point 1
has responded positively, shall be at least one of the following examinations
on samples taken at least seven days after the sampling referred to in
(a). (d)):
3.1. examination on the buffered Brucella Antigen test (test with
rose Bengal),
3.2. sérumaglutinační test,
3.3. the complement fixation test,
3.4. cELISA,
3.5. iELISA.
Suspected brucellosis shall be excluded if:
I) has either been repeated the examination referred to in paragraph 1 carried out with negative
as a result, the epidemiological investigation in the farm of origin did not reveal
the presence of porcine brucellosis and the examinations referred to in paragraph 3 has been carried out
with a negative result; or
II) the epidemiological inquiry in the holding of origin did not reveal the presence of
porcine brucellosis and all animals in tests referred to in points
1 or 3 has responded positively, have undergone a post-mortem examination and
tests for determining the causative agent of swine brucellosis in both cases with the
a negative result.
After the suspicion of brucellosis will exclude all animals may be of
isolation referred to in subparagraph (a). (d)) the second paragraph adopted by the Centre for
the collection of semen.
(2) all tests must be carried out in an approved laboratory.
(3) animals may be in a Centre for the collection of semen taken only with
the express permission of the Centre veterinarian. All
the movement of animals to and from the centres must be registered.
(4) no animal admitted to the semen collection centre shall on the day of
the adoption of the exhibit no clinical sign of disease; without prejudice to the provisions of
paragraph 5 should all animals coming directly out of isolation by
paragraph 1 (b). and), which on the day of dispatch fulfil the following
terms and conditions:
and was not in the area) with restrictions defined by Decree No.
299/2003 Coll., Act No. 389/2004 Coll. or Decree No. 202/2004 Sb.
due to the presence of the disease in pigs;
(b)) has not been in it in the last 30 days reported no clinical,
serological, virological evidence of Aujeszky's disease or pathological.
(5), provided that the conditions laid down in paragraph 4 and
during the previous 12 months were carried out routine tests referred
in part B, animals may be moved from one of the approved
semen collection centre to another of equivalent health status
without isolation or testing, if it's a direct transfer. The relevant
the animal must not come into direct or indirect contact with cloven-hoofed animals with
lower health status and the means of transport used must be
cleaned and disinfected.
(6) for the purposes of paragraph 5 and, in the case of trade between Member States,
be accompanied by a veterinary certificate for breeding pigs in
accordance with the model laid down by the legislation of the European Union.
(B)
Compulsory routine tests for swine semen collection centres
(1) the compulsory routine tests shall be carried out as follows:
and all the animals) held in an approved Centre for the collection of semen
must have undergone with negative results the following tests:
1. as regards brucellosis, the tests for brucellosis with buffered
brucelovým Antigen (rose Bengal test), or the cELISA or iELISA;
2. as regards Aujeszky's disease
2.1. in the case of non-vaccinated animals testing ELISA to detect
antibodies to the whole Aujeszky's disease virus or its glycoprotein B
(ADV-gB) or glycoprotein D (ADV-gD) or a serum neutralisation
the examination,
2.2. in the case of animals vaccinated gE GI deleted vaccine,
ELISA tests for detection of antibodies to glycoprotein E (ADV-gE) virus
Aujeszky's disease;
3. as regards classical swine fever ELISA tests for antibodies or
a serum neutralisation tests;
(b) the tests set out in (a)). and) must be made on samples
taken:
1. from all the animals immediately before leaving the Centre for subscription
semen, or immediately after arrival at the slaughterhouse and in any event no later
than 12 months after the date of adoption of the semen collection centre, or
2. every three months from at least 25% of the animals at the Center for a subscription
semen and veterinarian centers for the collection of semen must guarantee
the animals, from which the samples are taken, represent all the
population centres, particularly in terms of age group, and housing;
(c)) performs the examination in accordance with subparagraph (a). (b) point 2 shall)
the veterinarian Centre for the collection of semen to ensure that
all animals examined in accordance with subparagraph (a). and at least once in the process)
their stay at the Center for semen collection and at least once a
twelve months from the date of receipt, if their stay exceeds twelve
months.
(2) all tests must be carried out in an approved laboratory.
(3) if any of the above tests is positive, the animal must be
quarantined and sperm collected from it since the last time
a negative test may not be the subject of trade in the European Union.
Sperm of each animal at the Centre, which was removed in time from
the last negative test, is kept separately and may not be up to the
restore the health status of the Centre County veterinary services
the subject of emissions trading in the European Union.
Annex No 6 to Decree No. 380/2003 Coll.
ANIMAL HEALTH REQUIREMENTS FOR SWINE SEMEN COLLECTED IN CENTER FOR THE COLLECTION OF
SEMEN AND FOR TRADING IN THE EUROPEAN UNION
(1) the semen must have been obtained from animals which:
and on the day of collection of the semen) do not show any clinical sign of disease;
(b)) are not vaccinated against foot-and-mouth disease;
c) they meet the requirements of annex 5 to this Decree;
(d)) are not used to naturally;
e) are kept in semen collection centres which must not be
located in an area with a restriction in accordance with Decree No. 299/2003 Coll., Decree
389/2004 Coll. or Decree No. 202/2004 Coll., due to the occurrence of the disease in
pigs;
(f)) are kept in semen collection centres which was not in the
the period of 30 days immediately prior to collection of recorded no clinical,
serological, virological or pathological symptom of Aujeszky's disease.
(2) to the diluent or to the semen after final dilution must be added
an effective combination of antibiotics, in particular against leptospires. In the case of
frozen semen, antibiotics must be added before freezing.
and the effectiveness of this combination of antibiotics) must be at least equivalent to
the following concentrations in the final diluted semen:
at least 500 µ g streptomycin per ml final dilution,
500 IU penicillin per ml final dilution,
150 µ g lincomycin per ml final dilution,
300 µ g spektinomycinu per ml final dilution.
(b)) immediately after addition of the antibiotics the diluted semen must be kept at
a minimum temperature of 15 ° c, for at least 45 minutes.
(3) the semen for trading within the European Union must be:
and to dispatch) stored under the conditions laid down in part A, point 2
(a). (d)), and part B, point 6 (a). a), b), (e)), and (f)) of annex 4 to this
the Decree;
b) transported to the Member State of destination in containers which have been before the
cleaned and disinfected or sterilized and before
to dispatch from the approved storage facilities sealed.
(4) the State Veterinary Administration may refuse to accept on the territory of the United
Republic of sperm from semen collection centres which are
recruited boars vaccinated against Aujeszky's disease ".
28. in annex 7, section A (a). (g)) the first indent of paragraph 2, section B. 1
(a). about) the fourth indent of section (B) of paragraph 1. 1 (b). about) the last
paragraph, the word "oocytes" shall be replaced by "of the egg cells".
29. in annex 7, section A (a). (g)) of the second indent, the words "oocytes,
seed "shall be replaced by the words" egg cells, sperm "and the word" seed "
replaced by the word "semen".
30. In annex 7, section A (a). (h)), the word "oocytes" shall be replaced by
"egg cells".
31. in annex 7, section B, paragraph 2. 1 (b). q) with the word "oocytes"
replaced by the words "egg cells".
32. In annex 7, section B, paragraph 2. 2 the word "seed" shall be replaced by
"semen".
33. In Annex 8, paragraph. 3 the words "egg oocyte recovery
the cells "are replaced by" machines "of the egg cells.
34. Annex No 9 is added:
"Annex 9 to Decree No. 380/2003 Coll.
CONDITIONS FOR THE APPROVAL OF SEMEN COLLECTION CENTRES FOR SHEEP, GOATS, OR
EQUIDAE, SEMEN STORAGE CENTRES OF SHEEP, GOATS, OR
EQUINE EMBRYO COLLECTION TEAM OVINE, CAPRINE, PORCINE OR EQUINE ANIMALS AND
PRODUCTION TEAM FOR EMBRYOS OF THE OVINE, CAPRINE, PORCINE OR EQUINE ANIMALS, CONDITIONS
FOR SURVEILLANCE AND VETERINARY REQUIREMENTS FOR THE COLLECTION OF SEMEN OF THE OVINE, CAPRINE AND
EQUIDAE AND THE SUBSCRIPTION OF THE EGG CELLS AND EMBRYOS OF THE OVINE, CAPRINE, PORCINE AND
EQUIDAE
And
Conditions for the approval of semen collection centres for sheep, goats, or
equidae, semen storage centres of sheep, goats, or
Equine embryo collection team ovine, caprine, porcine or equine animals and
production team for embryos of the ovine, caprine, porcine or equine animals, and supervision in the
them
I. conditions for the approval of semen collection centres and centres for
storage of semen
Centre for the collection of semen
(1) the semen collection Centre shall be under the supervision of the veterinary
the doctor of the Centre.
(2) the semen collection Centre must have at least:
a) lockable animal housing and, if necessary, the enclosure for
equidae, physically separated from the semen collection device and from the
a room for its treatment and storage;
(b)) device to quarantine animals has no direct communication with the normal
ustajovacími spaces;
(c) facilities for the collection of semen), which provides protection against
adverse weather conditions, with a non-slip floor that protects
against injury to persons in the event of a fall at the point of collection of the semen and the surrounding area;
d) a room for the cleaning and disinfection or sterilization of equipment;
(e) a room for the treatment of semen) separate from facilities for the collection and
a room for cleaning equipment referred to in point (a). (d)), which does not need
necessarily be on the same site;
(f)) a semen storage room which need not necessarily be on the
the same place.
(3) the semen collection Centre must be constructed or isolated
so, to avoid the contact with animals outside.
(4) the semen collection Centre must be constructed in such a way that it is
the entire centre except the Office rooms and, in the case of equidae, in addition to the Paddock
easy to clean and disinfect.
Semen storage centre
(1) a semen storage Centre must have a specific registration
the number for each of the species whose semen is at the Centre
stored, if storage is not limited to the sperm of the individual
animal species subscribed in an approved semen collection centre
or if you are in the Center for storage of semen stored embryos
in accordance with this Decree.
(2) a semen storage Centre must be supervised
the veterinarian Centre.
(3) a semen storage Centre must have a room for the storage of
semen, equipped with the necessary amenities for semen storage centres,
where appropriate, the embryos that is designed so as to ensure that these products and
devices are protected from the adverse effects of the environment and
the weather.
(4) a semen storage Centre must be constructed in such a way as to
avoid contact with economic or other animals from outside.
(5) a semen storage Centre must be constructed in such a way as to
the whole centre except the Office rooms and, in the case of equidae, in addition to
range easy to clean and disinfect.
(6) a semen storage Centre must be constructed in such a way as to
effectively prevent access to unauthorized persons.
II. conditions for the supervision of semen collection centre and the Centre for
storage of semen
Centre for the collection of semen
(1) in a semen collection centre must be exercised supervision so that the
and) ensure that the resort staying only animals of the species
whose semen is collected.
Other animals, however, may be taken if there is no
risk of infection for animals of the species from which the sperm, and
If they meet the conditions laid down by the Centre veterinarian.
If, in the case of equidae, the Centre shares the site with subscription
artificial insemination or natural Service Centre, Mares, stallions
teaser stallions and stallions for natural service recruited, if
meet the requirements of part B, section I, paragraphs 1 to 4;
(b)) to prevent the entry of unauthorized persons. If it is enabled, visit
may only be taken under the conditions laid down by the veterinarian
the Centre;
(c)) was engaged only personnel who possess the basic knowledge in the care of
animals and is adequately trained in matters of disinfection and
hygiene practices, enabling the spread of diseases.
(2) the semen collection centre must be ensured by monitoring the
and record-keeping) which allow to determine:
1. the species, breed, date of birth and identification of each animal
present in the Centre,
2. any movement of animals entering the Centre and
leaving the Centre,
3. the health history and all diagnostic tests and the results thereof,
treatment and vaccination conducted in animals,
4. date of collection and treatment of semen,
5. destination of semen,
6. the storage of semen;
(b)) that no animal in the Centre was not held for at least 30 days
prior to the first semen collection and during the reception period used for
natural breeding;
(c)) to the collection, processing and storage of semen is carried out only in the
spaces reserved for this purpose;
(d)) that all instruments which come into contact with the semen or the
the donor animal during collection or treatment of the semen, have been either before
are properly disinfected or sterilised prior to use, with the exception of instruments
new, one-off and deleted after use, unless, in the case of
the equine Center subscription shares the space with a place where artificial
artificial insemination or natural service, must be the sperm, and tools and equipment for
artificial insemination or natural service consistently separated from
tools and equipment coming into contact with the donor animals or
other animals held in the Centre for collection;
(e)), to products of animal origin used in the treatment of semen,
such as diluents, additives or fillers, do not represent any
animal health risk or are so treated prior to use that
It was such a risk is excluded;
(f)) to freezing the mixture used for the manufacture or storage of semen
have not been previously used for other products of animal origin;
g) to storage and transport container before each filling have been duly
disinfected or sterilized prior to use, except for new,
one-off and deleted after use;
(h)) to ensure that the indication of each individual dose of semen
or each ejaculate of fresh semen intended for further
processing, to determine the date of collection, the species, the breed and
the identification of the donor animal and the veterinary approval number of the
approved Centre, which has collected the sperm.
(3) in a semen collection centre must be made the official
veterinarian during the breeding period of at least one
a year in the case of animals with seasonal reproduction, and twice a year in the case of
nonseasonal reproduction in order to assess and validate all matters
regarding the conditions of approval and supervision, as appropriate, on the basis of
records, standard operating procedures and internal audits.
Semen storage centre
(1) a semen storage centre must be exercised supervision,
in order to
and) ensure that the health status of the donor animals whose
sperm is stored in the Centre, meets the requirements of this order;
(b)) to prevent the entry of unauthorized persons. If it is enabled, visit
may only be taken under the conditions laid down by the veterinarian
the Centre;
(c)) was engaged only personnel who possess the basic knowledge in the care of
animals and is adequately trained in matters of disinfection and
hygiene practices to prevent the spread of disease;
(d)) ensure that records are kept of all movement of semen
received and leaving the storage centre.
(2) a semen storage centre must be ensured by monitoring,
in order to
and to an approved Centre) the storage of sperm, only be accepted
that was removed, and it comes from an approved Centre for the subscription
sperm or semen storage centre, has been
transported under conditions guaranteeing any animal health guarantees, and
without coming into contact with the semen that don't meet the conditions of this order;
(b)) the semen was under strict hygienic conditions stored only in
spaces reserved for this purpose;
(c)) all instruments which come into contact with the semen, have been before
are properly disinfected or sterilised prior to use, except for the tools in the
single use;
d) storage and transport container before each filling have been duly
disinfected or sterilized except for single-use containers;
e) freezer mixture used for the manufacture or storage of semen
have not been previously used for other products of animal origin;
f) each dose of semen is clearly marked in such a way that it is possible
easily determine the date of collection, the species, the breed and identification of the donor
the animal and the veterinary approval number of the approved Centre.
(3) the Centre for the storage of semen can by way of derogation from paragraph 1(b), 2 (a). and)
stored embryos if the embryos meet the requirements of this order and
they are stored in separate containers.
(4) a semen storage centre must be ensured in order to
semen storage centre controlled by official veterinary
doctor at least twice in each calendar year, to analyze and
Verify all the aspects relating to the conditions governing the approval, surveillance and
monitoring, as appropriate, on the basis of records, standard operating
procedures and internal audits.
III. Conditions for the approval and supervision of embryo collection teams and teams
for production teams
The embryo collection team
(1) the collection, processing and storage of embryos shall either veterinarian
team, or under his responsibility by one or more of the eligible engineers,
veterinarian trained in methods and techniques and
in the techniques and principles of disease control.
(2) the team veterinarian is responsible for all the activities of the team, IE. outside of the
other in:
identity and authentication) the health status of donor animals;
(b) sanitary safety of care) donors and surgical
procedures;
(c) disinfection and hygienic procedures);
(d)) the keeping of records, which recorded:
1. the species, breed, date of birth and identification of each of the donor
of the animal,
2. health records, all diagnostic tests and the results thereof,
treatment and vaccination of donors,
3. place and date of collection, processing and storage of egg cells and
embryos,
4. the identification of the embryos, and the details of their place of destination,
If they are known.
(3) the Team shall be subject to the general supervision by the official veterinarian
doctor who control at least once in each calendar year
ensure compliance with the sanitary conditions for the collection, treatment and
storage of embryos and verifies all the aspects relating to the terms and conditions
approval and supervision as appropriate on the basis of records, standard
the operating procedures and internal audits.
(4) the team must have on the investigation, treatment and storage of embryos available
permanent or mobile laboratory that has at least a work surface,
optical or electron microscope and freezing equipment as needed.
(5) the permanent laboratory shall have:
and room processing of embryos), physically separate from the area for
subscriptions from donor animals;
(b)) a room for the cleaning and sterilization of the instruments, if not used
single use;
(c) a room for the storage of embryos).
(6) mobile laboratory must:
and have specially equipped) part of the vehicle, divided into two separate
sectors:
1. sterile-the investigation and processing of embryos and
2. the drop-on equipment and materials used in contact with the donor
animals;
(b)) use only single-use equipment, unless it can be combined with
permanent laboratory to ensure sterilization of equipment and the supply of fluids and
other products needed for the collection and processing of embryos.
(7) the construction and layout of the buildings and laboratories and the implementation of the
the activities of the team must be such as to guarantee mutual protection
contamination of the embryos.
(8) the team shall have available storage space to allow for:
and to earmark at least one) a lockable room for the storage of
the egg cells and embryos;
b) easy to cleanse and disinfect;
c) permanent records of all incoming and issued by egg
cells and embryos;
(d) to store the container on egg) cells and embryos on the site of a controlled
by the team veterinarian and regularly controllable official
veterinarian.
(9) a semen storage room in the storage rooms referred to in paragraph 8
may be permitted by the competent authority under the conditions that:
a) sperm meets the requirements of this order for sheep, goats or equines
or the requirements of this order for trade in porcine semen and for
its imports;
(b) for the purposes of) semen is stored in separate storage team
containers in areas approved for the storage of embryos.
The embryo production team must, in addition to the requirements for an embryo collection team
also comply with the following additional conditions:
(1) the members of the team are properly educated in the areas of disease control and
laboratory methods, in particular as regards the procedures work in sterile
conditions.
(2) the team has at its disposal a permanently sited laboratory, that:
and has the proper equipment and) accessories, including separate rooms
for:
1. isolation of egg cells from the ovary,
2. the processing of the egg cells and embryos,
3. the storage of embryos;
(b)) has a device with a laminar flow or other suitable device to
technical operations in specific sterile conditions (processing
the egg cells, embryos and semen).
Semen centrifugation can be in compliance with the hygiene conditions
made outside the laminar-flow devices or other devices.
(3) in the event that the egg cells and other tissue collected at the slaughterhouse,
slaughterhouses must have adequate facilities for the hygienic and safe
the collection and transport of ovaries and other tissues to the laboratory for treatment.
(B)
Requirements for the animals accepted into the semen collection centre for sheep,
goats or equines
Male-donor requirements
I. Stallions
To collection of the semen may be used only Stallion who satisfies the
the following requirements of a certified veterinarian centers:
(1) not at the reception and on the day of collection of the semen have no
the symptoms of the disease.
(2) they must come from the territory or, in the case of a regional breakdown of the parts of the
territory of a Member State or a third country and from a holding under veterinary
supervision that meet the requirements of the Decree No 382/2003 Coll.
animal health requirements for trade in animals and animal health
the terms of their imports from third countries, as amended.
(3) must be over a period of 30 days prior to semen collection in holdings,
in which during this period do not show no equidae any
clinical signs of infectious equine Arteritis or contagious metritis
Mares.
(4) it shall not be for a period of 30 days prior to the first semen collection and during the
the sampler used for natural breeding period.
(5) shall be subjected to the following examinations in accordance with the programme
laid down in paragraph 1. 6 and carried out in an approved laboratory:
and agar gel Immunodiffusion)-test (Cogginsův test) or ELISA test
for infectious anaemia with negative result;
(b)) a virus isolation test for infectious Arteritis with negative
the result of the carried out on the aliquot portions of all of the semen of the donor
the Stallion, unless it has a negative result is obtained by a serological
examination with a virus neutralization test for equine Arteritis in the serum dilution
1:4;
(c)) a test for contagious equine metritis carried out on two consecutive
samples taken in the interval be stallions seven days in isolation
the bud of Taylorella equigenitalis from pre-ejaculatory fluid or a
a semen sample and from genital swabs taken at least from the fossa
urethalis, including urethalis, as well as the sine of the penis, including the fossa
glandis, in all cases with negative results.
(6) shall be subjected to one of the following programs:
and if the donor Stallion was continuously) resided in the subscription center
semen for at least 30 days prior to the first semen collection and during the period
subscription, and if no equidae on the collection of semen did not come
into direct contact with equidae of lower health status, with a
the donor Stallion, the tests shall be carried out in accordance with paragraph 1. 5 on the samples
taken be stallions prior to the first semen collection and at least 14
days after the start date of stay lasting at least 30 days;
(b)) if the donor Stallion resided continuously in the subscription center
semen for at least 30 days prior to the first semen collection and during the period
subscription, but can occasionally leave the Center with the approval of
veterinarian station on a 24-hour period of less than 14 days,
or other equidae on the collection of semen
come into direct contact with equidae of lower health status,
the tests shall be carried out in accordance with paragraph 1. 5 be on samples taken
the stallions, as follows:
1. at least once a year at the beginning of the breeding season or before the first
semen collection and at least 14 days after your initial date of visits
at least 30 days,
2. during the period of semen collection:
2.1. for the test referred to in paragraph 1. 5 (b). and) at least once every 90 days,
2.2. the test referred to in paragraph 1. 5 (b). (b)) at least once every 30 days, in addition to
the case, to a virus isolation test which must be carried out twice a year,
is confirmed as seropositive for infectious Arteritis in horses, Stallion
2.3. for the test referred to in paragraph 1. 5 (b). (c)) at least once every 60 days;
(c)) if the donor Stallion does not meet the conditions referred to in points (a)), and
(b)) or if the collection of semen for the purpose of trade is carried out
frozen semen, the tests shall be carried out in accordance with paragraph 1. 5 on the samples
taken be stallions as follows:
1. at least once a year at the beginning of the breeding season,
2. during the period of storage referred to in part C, chapter I (2) 3 (b). (b))
and before you delete the semen from the Centre or the use of,
collected 14 to 90 days after the date of collection of the semen.
By way of derogation from point 2 is not a procedure for samples after sampling and tests for infectious
Arteritis horses described in paragraph 1. 5 (b). (b)) required that the test
on the isolation of the virus must be carried out twice a year, is confirmed as
seropositive for infectious Arteritis in horses Stallion.
(7) if any of the tests referred to in paragraph 1. 5 positive, must be
the donor Stallion was isolated and the semen collected from it since the last data
negative test will not be the subject of trade; the exception, in the case of
infectious Arteritis of horses is sperm from every ejaculate which has been
subjected to a virus isolation test for infectious Arteritis with negative
as a result.
Semen collected from all other stallions at the Centre for the subscription
semen from the date of subscription to the last sample with negative results,
one of the tests referred to in paragraph 1. 5 is stored separately and may not be
traded, until the health status of the semen collection centre will not be
restored and stored sperm will not be subjected to the appropriate official
the examination, which will rule out the presence of pathogens causing
the diseases listed in paragraph 1. 5.
(8) the semen collected from stallions at the Center for the collection of semen, who
shall be subject to the prohibition in § 19 para. 2 Decree No 382/2003 Coll., is saved
separately and may not be traded until the health status of the Centre
for the collection of semen will not be restored by an official veterinarian, and
the stored sperm will not be subjected to the appropriate official examination,
that will rule out the presence of pathogens causing diseases of horses
which are compulsorily notifiable in accordance with Annex 1 to the Decree No 382/2003
SB.
II. Sheep and goats
(1) for all Rams and qəzli admitted to the semen collection centre
the following conditions apply:
and) for at least 28 days were placed to the isolation in which they are located
only animals of at least the same health status;
(b)) before placing into isolation were holding keeping she-goats and sheep
tits officially brucellosis in accordance with annex of the sheer No. 24 of Decree No.
299/2003 Coll. and were previously kept in farms with lower health
status, as regards brucellosis;
(c)) come from a holding where during the 60 days before placing it in the
isolation have undergone a serological test for epididymitis (Brucella Rams
Ovis) carried out in accordance with Annex D to Directive 91/68/EEC or other
test of equivalent sensitivity and specificity;
d) have undergone the following tests of blood samples taken in the course of the
28 days before the beginning of isolation by (a). and in all cases), with
negative results, with the exception of border disease virus of sheep
According to point 3 of the sub-item 3.2.:
1. for brucellosis (Brucella melitensis)-serological test carried out in
accordance with Annex C to Directive 91/68/EEC;
2. on the Rams epididymitis (Brucella ovis) a serological test referred to in-
Annex D to Directive 91/68/EEC or another test of equivalent sensitivity and
specificity;
3. on the border disease of sheep:
3.1. virus isolation test or virus Antigen test to demonstrate,
3.2. a serological test to determine the presence or absence of the
antibodies (antibody test).
The regional Veterinary Administration may allow the execution of the tests referred to in
This point on samples taken in isolation. If the authorisation
released, he cannot (a) isolation period. and start before the date of collection)
samples. However, if the result of one of the tests referred to in this point
is positive, the animal concerned shall be immediately removed from isolation. In
the case of group isolation cannot time isolation by (a). and) start for
other animals before the animal with a positive result of the test
removed;
(e)) at least 21 days after being admitted to isolation, with negative results were
subjected to the following tests of samples taken in the course of isolation by
(a). and):
1. for brucellosis (Brucella melitensis)-serological test carried out in
accordance with Annex C to Directive 91/68/EEC;
2. on the Rams epididymitis (Brucella ovis) a serological test referred to in-
Annex D to Directive 91/68/EEC or another test of equivalent sensitivity and
specificity;
(f)) have undergone tests for border disease of sheep (a). (d) point 3)
the sub-sections 3.1. and 3.2., carried out on blood samples taken in the
during the isolation by (a). and) at least 21 days after being admitted to isolation.
Any animal (seronegative or seropositive) may be
allowed in the semen collection centre only in the case that
No seroconversion in any animal which was prior to the entry into
isolation of seronegative.
Sero-conversion occurs, all animals must remain
seronegative, remain in isolation for as long as this group has already
no more seroconversion for three weeks.
Serologically positive animals may be to the semen collection centre
accept the condition of the negative result of the test according to (b). (d) point 3)
subsection 3.1.
(2) animals into semen collection centres shall be adopted only with the
the express consent of the Centre veterinarian. All movements of the
the Centre for the collection of semen and it must be recorded.
(3) no animal admitted to the semen collection centre shall on the day of
the adoption of no clinical signs of the disease appear. Without prejudice to paragraph 2. 4,
all animals received must come from isolation, that on the day of departure
animals into semen collection centres meets the following conditions:
and in the field) is located, which in the last 30 days there has been no case of
foot-and-mouth within a radius of 10 km;
(b) at least 3 months) did not occur in isolation to the incidence of brucellosis, foot and
mouth disease;
(c)) at least 30 days been free from the disease in the isolation of ovine or caprine animals
subject to compulsory reporting in accordance with Annex 1 to the Decree No 382/2003
SB.
(4) subject to the conditions referred to in paragraph 1. 3 and 12 months
before moving the animals were carried out routine tests according to paragraph 2(a). 5, can be
animals transferred from one approved semen collection centre to
the second is of equal health status, without isolation or testing,
If the transport direct. The animal must not come into direct or
indirect contact with cloven-hoofed animals of lower health status and used
the means of transport must be disinfected before use. The transport of animals
from one semen collection centre to another in another Member
the State must be carried out in accordance with Decree No 382/2003 Coll.
(5) all ovine and caprine animals located in an approved Centre for the
the collection of semen shall be at least once in each calendar year
subjected to the following tests with negative results:
and) on brucellosis (Brucella melitensis)-serological test carried out in
accordance with Annex C to Directive 91/68/EEC;
(b)) to the Rams epididymitis (Brucella ovis)-serological test carried out
in accordance with Annex D to Directive 91/68/EEC or other equivalent test
sensitivity and specificity;
(c)) on the border of the disease of sheep-test for antibodies. 1 (b). (d))
section 3 subsection 3.2. shall be made only to seronegative animals.
(6) all tests referred to in this section must be carried out by approved
laboratory.
(7) if the result is one of the tests referred to in paragraph 1. 5 positive,
the animal must be isolated and the semen collected from it since the last
a negative test result may not be the subject of trade.
This animal must be from the semen collection centre, with
except in the case of border disease of sheep, the animal must undergo a test
According to paragraph 2(a). 1 (b). (d) subsection 3.1 section 3). with a negative result.
Semen collected from all other animals at the Center for a subscription
semen from the date of subscription to the last sample with negative results,
one of the tests referred to in paragraph 1. 5 is stored separately and shall not be subject to
trading until the health status of the semen collection centre will not be
restored and stored sperm will not be subjected to the appropriate official
the examination, which will rule out the presence of pathogens causing
the diseases listed in paragraph 1. 5.
(8) the Sperm may only be removed from animals which:
and showed no clinical signs) of disease on the day of collection of the semen;
b) during the 12 months before the date of collection of the semen:
1. either have not been vaccinated against foot-and-mouth disease, or
2. they have been vaccinated against foot-and-mouth disease for at least 30 days before the
removal; in this case, must be 5% (with a minimum of five straws) of each
the semen is subjected to a virus isolation test for foot-and-mouth, with
a negative result;
c) in the case of fresh semen collection was continuously for at least 30 days
before the date of the subscription are kept in an approved semen collection centre;
(d)) meet the requirements provided for in § 15, 15a and 15b of the Decree No 382/2003
SB.;
(e)) where she was reared on holdings referred to in paragraph 12 (a). and)
she underwent during the 30 days prior to semen collection, with negative results
These tests:
1. serological test for brucellosis (Brucella melitensis) carried out in
accordance with Annex C to Directive 91/68/EEC,
2. serological test on epididymitis of Rams (Brucella ovis) carried out in
accordance with Annex D to Directive 91/68/EEC or other equivalent test
sensitivity and specificity,
3. test the virus disease of sheep on the border;
(f)) was not used for the purposes of natural breeding at least 30 days before the
date of the first collection of the semen and between the date of the first sampling by
paragraph. 1 (b). e) and (f)) or (b). (e)), and by the end of the collection period.
(9) the semen collected from the donor Rams and goats at the Center for
the collection of semen, or in the holding referred to in paragraph 12 (a). and)
for health reasons is subject to a prohibition in accordance with § 15
Decree No 382/2003 Coll., is stored separately and shall not be subject to
trading until the health status of the semen collection centre will not be
restored by an official veterinarian and stored sperm is not subject to
the appropriate official examination, which will exclude the presence
pathogens causing disease ovine or caprine animals subject to compulsory
report in accordance with Annex 1 to the Decree No 382/2003 Coll.
(C)
Requirements for semen of the ovine, caprine or equine species and egg cells and
the embryos of the ovine, caprine, porcine or equine animals
I. requirements on the collection, processing, preservation, storage and transport
semen
(1) where, in accordance with the law on pharmaceuticals added antibiotics
or their mixtures with bactericidal action in each milliliter of semen
at least equal to baktericidnímu the effect of the mixture of gentamicin (250
µ g), tylosin (50 µ g), lincomycin-spectinomycin (150/300 µ g), penicillin
(500 IU), streptomycin (500 µ g), lincomycin-spectinomycin (150/300 µ g),
amikacin (75 µ g) or divekacin (25 µ g), must be the name of antibiotics
together with the concentrations indicated in the veterinary certificate.
(2) all instruments used for the collection, treatment, storage, or
freezing semen must be properly disinfected before use or
sterilized prior to use, except for single-use instruments.
(3) frozen semen shall be:
and) saved and stored in the storage containers:
1. which have been cleaned and disinfected prior to use or
sterilized prior to use, except for single-use containers
2. with a freezing mixture that has not been previously used for other products
of animal origin;
(b) prior to the transport or use of) stored under approved conditions for
at least 30 days from the date of subscription.
(4) the sperm, which is to be the subject of trade, must be:
and) transported to the Member State of destination in containers which
they were cleaned and disinfected before use or sterilised prior to use, with
except for single-use containers, and which have been sealed and numbered
prior to dispatch from the approved semen collection centres or
Centre for storage;
(b)) were marked so that the number on pejetách or other storage vessels
match the number indicated in the veterinary certificate and with container, in
which is stored and transported.
II. conditions for egg cells and embryos
(1) the collection and treatment of embryos obtained in vivo
Embryos obtained by in vivo is considered to be the result of artificial insemination
semen which meets the requirements of this order, and for their subscription,
treatment and conservation requirements are as follows:
and the collection and treatment of embryos) performed by approved embryo collection team,
While the embryo must not come into contact with any other dose of embryos,
which do not meet the requirements of this Ordinance;
(b)) collection is carried out in a place which is separated from the other
parts of the Centre or the economy is in good shape and construction solutions
allows for efficient and easy to clean and disinfect;
(c) the processing of the embryos (investigation), washing, care and storage of the
identified and sterile straws, ampoules or other receptacles) shall be carried out
in stationary or mobile laboratory, for susceptible species situated in the
the area, which in the last 30 days there has been no case of foot
-and within a radius of 10 km;
d) all equipment used for the collection, handling, washing, freezing, and
storage of embryos must be sterilized before use either or properly
cleaned and disinfected according to the international society for
embryo transfer society (IETS), or must be disposable;
(e)) any biological product of animal origin used in the media and
solutions for the collection, handling, washing or storage of embryos must be
plain pathogenic micro-organisms. Media and solutions used for the collection,
freezing and storage of embryos must be approved by
sterilized in accordance with the manual of the international society for the transmission of
embryos (IETS) and handling must be carried out so as to make their
sterility is maintained. In accordance with the manual of the international society for
embryo transfer society (IETS), you can turn to the media for the collection, processing,
washing and storage add antibiotics;
f) freezer mixture used for the manufacture or storage of embryos shall not
have been previously used for other products of animal origin;
g) any straw, ampoule or other receptacles with embryos must be clearly
labelled according to a standardised system of international
embryo transfer society (IETS);
h) washing the embryos must be carried out according to the International
embryo transfer society (IETS) and before him, after him, even in his
the course must not be impaired by the zona pellucida. If you need to inactivate or
remove specific viruses, can be the standard washing procedure should be amended to
It covers more washing with the enzyme trypsin in accordance with international
embryo transfer society (IETS);
I) embryos from different donor animals must not be washed together;
(j) the zona pellucida of each embryo) must be examined over its entire surface
at least 40 by magnification and it must be confirmed that there is
intact and free of adherent of foreign particles;
the embryos from the levy to) tested in accordance with subparagraph (a). (j)) are stored in a sterile
straws, ampoules and other containers referred to in subparagraph (a). (g)) and immediately seal;
l) each embryo intended for freezing must be frozen without delay and save
on the site, which is under the control of the team veterinarian.
m) each embryo collection team shall be presented to the official examination
bacterial and viral contamination of the routine life of the unfit specimens
embryos or egg cells, purging or cleaning fluids on the
the basis of its activities under the guide of the international society for the transmission of
embryos (IETS);
n) each embryo collection team must keep a record of their activities, if
as for the collection, and the store is two years after the sale, or
imports of embryos. Records contain the following information:
1. the breed, age and identification of the donor animals concerned;
2. the place of collection, processing and storage of embryos collected by the team;
3. identification of the embryos, and the details of their recipients.
(2) the collection and processing of egg cells of the ovaries and other tissues to
the production of embryos obtained in vitro the conditions laid down in paragraph 1. 1 (b).
a) to (n)) shall apply mutatis mutandis for the collection and processing of egg cells
the ovaries and other tissues for in vitro fertilization and culture in vitro.
In addition, the following conditions shall apply:
and) the competent authority must know the holding of donor animals
come and have the appropriate powers to him;
(b)) in the case that the ovaries and other tissues shall be taken at the slaughterhouse, and it
from either individual animals or groups of animals (hereinafter referred to as "group
subscription "), the slaughterhouse must be officially approved by regulation of the European
Parliament and of the Council (EC) No 854/2004 of 29 April 2004. April 2004 laying
laying down specific rules for the organisation of official controls on products of
animal origin intended for human consumption, as amended, and shall
be under the supervision of a veterinarian whose responsibility it is to ensure
to have been made of the potential donors before tours
ante-and post-mortem inspection, and to confirm that these animals are free from symptoms
the relevant diseases transmissible to animals. The slaughterhouse must lie in an area in
which, as far as the susceptible species, for the last 30 days there has been no
case of foot-and-mouth within a radius of 10 km;
(c)) the benefits of the ovaries do not get into the processing laboratory until
is not made a tour of donor animals after slaughter;
(d) the equipment to remove the ovaries) and other tissues and their transportation
must be cleaned and disinfected or sterilized and
may be used solely for this purpose.
(3) the processing of the embryos obtained in vitro
The conditions laid down in paragraph 1. 1 (b). a) to (n)) shall apply mutatis mutandis for the treatment
embryos collected in vitro. In addition, the following conditions shall apply:
and embryos obtained by in vitro) shall be considered as the result of in vitro fertilization
semen which meets the requirements of this order;
(b)) after the in vitro culture of embryos before freezing must be,
storage and transport washed and treated in accordance with paragraph 1. 1 (b). h), (j)), and
k);
c) embryos from different donor animals, in the case of an individual
subscription, or from different group subscriptions may not be washed together;
d) embryos from different donor animals, in the case of an individual
subscription, or from different group subscriptions may not be stored in the same
Straw, ampoule or another stack.
(4) the processing of the embryos Before undergoing a micromanipulation of micromanipulation,
the zona pellucida is broken, it must be all the embryos or egg
the cells are removed and treated according to the sanitary conditions laid down in
paragraph. 1, 2 and 3. In addition, the following conditions shall apply:
micromanipulation of the embryo), which involves penetration of the zona pellucida
must be carried out in a suitable laboratory facilities, under the supervision of
the approved veterinarian team;
(b)) each embryo collection team shall keep records of its activities in accordance with
paragraph. 1 (b). n), including details of micromanipulation techniques which
the penetration of the zona pellucida is a part and which were carried out on the
embryos. The identification of the embryos obtained by in vitro fertilization may
to perform according to the benefits, however, you must include information about the date and place of sampling
the ovaries or egg cells and allow for the identification of
the holding from which the donor animals originated.
(5) the storage of embryos
and each team) for the collection and production teams must ensure that storage
embryos at appropriate temperatures, in the storage rooms referred to in part A
Chapter III, paragraph 1. 8.
(b)) frozen embryos must be stored under approved before sending
conditions for at least 30 days from the date of collection or production.
(6) transport of embryos
a) embryos, which are to be the subject of trade must be transported
in the Member State of destination in containers which have been before the
cleaned and disinfected or sterilized prior to use, or are the containers
single use, and which have been sealed and numbered prior to dispatch
from the approved storage centres.
b) Straw, ampoule or other receptacles must be marked so that the number
on pejetách, ampoules or other bunkers match the number referred to
in the animal health certificate and the container in which they are stored and
transported.
(D)
Requirements for female-donor
(1) for the use of the collection of the embryos or egg cells must the donor and
the holding from which they come, meet the requirements for
trade in animals of each species of Decree No 382/2003 Coll.
authenticated by the official veterinarian.
(2) in addition to the requirements laid down in sections 4 to 11 of the Decree No 382/2003 Coll. must
donor pigs, except for in vivo derived embryos treated with
trypsinem, comply with the conditions associated with Aujeszky's disease
set out in section 12 and 13 of the Decree No 382/2003 Coll.
(3) to the donor sheep and goats, the requirements of section 15 to 18
Decree No 382/2003 Coll.
(4) for the donor Mare shall apply in addition to the requirements laid down in section 19 to 25
Decree No 382/2003 Coll., the following conditions:
and) may not be used for the purposes of natural breeding at least 30 days before the
the date of collection of the egg cells or embryos, and in the period from the date of the first
sampling in accordance with subparagraph (a). (b)), and (c)) until the date of collection of the egg cells and
embryos;
(b)) must have a negative test result to an agar-gel immuno-diffusion
(Coggins test) or ELISA test for infectious
anaemia, carried out on blood samples taken first
during the last 30 days before the date of the first collection of egg cells or
embryos and then every 90 days throughout the collection period;
(c)) must submit to a test for contagious equine metritis insulation
Taylorella equigenitalis, carried out on samples taken from the
slizničního surface of the fossa clitoralis and sinus clitoralis in two
consecutive periods of estrus; in one period of rut should be examined also
a sample taken from the endocervix. all results must be after the cultivation of 7
up to 14 days. ".
35. Annex 10 shall be deleted.
Article. (II)
The effectiveness of the
This Decree shall enter into force on 1 January 2000. June 2013.
Minister:
Ing. Sarah b in r.
