415/2009 Coll. DECREE of 19 November 2009 establishing requirements for sampling and manner of publication of analytical methods for testing products to be fed to the Ministry of Agriculture determined in accordance with section 17 para. 9 of the Act No. 91/1996 Coll., On feed, as amended by Law No. 244/2000 Coll., Act No. 147/2002 Coll. and Act No. 214/2007 Coll.: § 1 (1) This Decree incorporates the European Community it is ^ 1) and adjusted in relation to the directly applicable regulations of the European Communities ^ 2)) for sampling (b)) the manner of publication of analytical methods testing of products that feed. (2) This Decree shall not affect the sampling or sampling frequency range set by the other legislation ^ 3). § 2. Samples intended for the official control of pesticide residues in food products to be collected and processed by the procedure set out in the Annex hereto. § 3 Publication of analytical methods for testing feed products ^ 4) in a manner allowing remote access by the Ministry of Agriculture. section 4 Transitional provisions Samples collected before the effective date of this decree shall be assessed in accordance with the existing legislation. section 5 Repealing provisions: 1. Ministry of Agriculture Decree No. 124/2001 Coll., Laying down the requirements for sampling and analytical methods for testing the principles of feed additives and premixes and method of storing samples. 2. Decree No. 497/2004 Coll., Amending Decree of the Ministry of Agriculture No. 124/2001 Coll., Laying down the requirements for sampling and analytical methods for testing the principles of feed additives and premixes and method of storing samples. 3. Decree No. 85/2006 Coll., Amending Decree of the Ministry of Agriculture No. 124/2001 Coll., Laying down the requirements for sampling and analytical methods for testing the principles of feed additives and premixes and method of storing samples, as amended Decree No. 497/2004 Coll. § 6 Effectiveness of This Decree shall take effect on December 1, 2009. Minister: Ing. Sebesta vr Attachment Procedure, extracting and processing the samples for the official control of pesticide residues in products to be fed this Part i. Concepts) Sampling is the process used to create a collection and sample. (b)) Sampling devices are instruments such as e.g. blade, knife or needle to remove and sub-sample from the bulk material or piece of material too large for it to be used as a partial sample, then the sample divider, and which are used to prepare a laboratory sample from the general sample or to prepare a test dose of the test sample and a special sampling device described in ISO and IDF ^ 1). For materials such as grazing menu can be considered for sampling equipment hand person performing the sampling. (c) An employee authorized it) of the sample and the person trained in sampling procedures and, where appropriate institution authorized to take samples and is responsible for the entire procedure, including preparation, packaging and shipping of the laboratory sample. Complies with established sampling procedures, maintain full documentation for samples, and works closely with the laboratory. (d)) The sample is part of the material taken from a larger quantity of material. The sample is representative of the game, the aggregate sample etc., in terms of pesticide residue content and not necessarily in terms of other properties. (e)), the sample size is the amount of the material forming the sample. (f)), The game is the amount of feed that is by the person performing the sampling of common characteristics, such as origin, producer, variety, packer, type of packaging etc. suspicious games is the game for which any reason exists to suspect that it contains excessive amounts of pesticide residues. (g)) The partial sample quantity taken from one randomly selected locations in the game. h) The aggregate sample is a unit formed by combining and carefully mixing large enough incremental samples taken from the lot. I) Laboratory sample is a representative quantity of material removed from the bulk sample sent to the laboratory, or the aggregate sample or part thereof. (j)) of the test sample material is separated from the laboratory, the sample to be analyzed, and adjusted subsequent mixing, crushing, pulverizing etc., which will take a test batch with the smallest possible sampling error. to test dose) is representative quantity of material drawn from the test sample, which is sufficient to determine the concentration of pesticide residues. Explanation: 1) International Standard ISO: cheese chocolate coffee confectionery-sampling (corn), International Standard ISO: Packaged pulses-International Standard ISO Sampling: Sampling-Tea, International Standard IDF: Milk and milk products-methods of sampling Part II. 1. Sampling (1) Each lot must be sampled separately. If the lot collected more samples should be pooled sample and subsamples represented in approximately equal shares. (2) The consignment may consist of one or more lots. The game may be mixed, for example due to and sorting or manufacturing process. If the shipment Consisting of parts from different providers, each batch must be considered separately. If in a large consignment is not clearly defined boundaries or the size of the individual parts can be regarded as a separate batch of each of a series of wagons, trucks or cargo space ship. (3) The sampling can be used sampling devices. Sub-samples are taken from a randomly selected location, if it is not physically possible, and a sample is taken from a random place in the accessible parts of the lot. If sub-samples are taken during the loading or unloading of the lot a few times, "site" means the collection and the point in time. Incremental samples must contain sufficient material that form and a laboratory sample and must be carried out so as to prevent contamination or damage to the sample, which could affect the analysis results. (4) the Minimum number of incremental samples taken from the lot for the determination of pesticide residues are as follows:-----------------------------------------------------------------------------------------------------------Free Loose or packaged feed, which may not be well mixed by homogeneous bulk or packaged or feed, it can be assumed that they are well intermingled or homogeneous-----------------------------------------------------------------------------------------------------------Weight lot Number of packages in the lot--------------------------------------------------------------------up to 50 kg 50-500 kg > 500 26-100 1-25 kg > 100-----------------------------------------------------------------------------------------------------------Number 1 3 5 101 5 10 subsamples--------------------------------------------------------------------------------- --------------------------(5) For products to be fed, which are presumed to be well mixed or homogeneous are considered especially oilseeds grains, seeds, premixes, compound feed and feed materials loose state, scrapping the cake and extracted meals and the like. For products that feed that may not be well mixed or homogeneous are considered particularly granular products that feed, forage mixture of whole grain, oil cake and extracted meals at original condition, forages, etc. (6) Sub-samples would be combining and proper mixing always form a single aggregate sample. If not form the aggregate sample mixing possible, one can proceed in the following alternative manner. If mixing or dividing the aggregate sample can damage the material (and thus it influence the content of residues), or if no mixing of the material it achieve a more equitable distribution of residues and to obtain a more representative laboratory samples randomly distributed material it replicate laboratory samples at taking incremental samples. In such a case, as a result of force applied the mean results obtained from the laboratory samples analyzed. (7) Sub-samples shall be large enough to allow the aggregate sample to remove all laboratory samples. If the individual laboratory samples are prepared by taking a subsample is considered the sum of the aggregate sample laboratory samples at the time of sampling the lot. (8) the Description of primary samples and minimum size of laboratory samples:--------------------------------------------------------------------------------------------Product Classification Form subsample that Min. size of the laboratory sample should be removed--------------------------------------------------------------------------------------------cheese chocolate coffee confectionery and pulses packaging material or 1 kg of collected sampling equipment--------------------------------------------------------------------------------------------tubers and roots (eg. potatoes, whole chunks of 1 kg beets) weighing 25-250 g (of at least 10 pieces)--------------------------------------------------------------------------------------------roots and tubers weighing over 2 kg of cuts (250 g , at least 5 pieces)--------------------------------------------------------------------------------------------Bulk animal material collected fat 0.5 kilograms sampling device from at least three sites--------------------------------------------------------------------------------------------liquid products (eg. milk, packaging materials or 0.5 liters and 0.5 kg vegetable oils) collected and sampling device--------------------------------------------------------------------------------------------Other feed materials (eg. the packaging material or 0.5 kg of oilseeds, gates, collected the sample extracted meals) device-------------------------------------------------------------------------------------------Feed mixtures or packing material removed 0.5 kilograms sampling device--------------------------------------------------------------------------------- -----------the additives and premixes or packing material removed 0.2 kilograms sampling device--------------------------------------------------------------------------------------------2. Statement of sampling employee authorized to take samples must record the nature and origin of the game, the name of the owner, supplier or carrier, the date and place of sampling, and any other information it is. Any deviation aggregate column from the recommended method of sampling must be recorded. One signed copy of the report must be attached to each replicate laboratory sample and one copy to be kept by an authorized person for sampling. A copy of the sampling the owner receives lots or his representative, regardless of whether they receive and the laboratory sample. If the sampling protocols acquired in electronic form, distributed to the same recipients and keep the copy verifiable. Part III. 1. Preparation of the laboratory sample If the aggregate sample is larger than is necessary to create a laboratory sample, separated so as to produce a representative part. In doing so, use the sampling device, quartering or other suitable sample shrinking process, but the fresh vegetable products may be cut or divided. If necessary, at this stage, collected or prepared in duplicate laboratory samples as described above. 2. Packaging and transport of laboratory samples Laboratory sample must be stored in a clean, inert container to ensure adequate protection from contamination, damage or leakage of the sample. The container should be sealed, labeled and securely must be accompanied by the sampling protocol. If a barcode is recommended it also specify alphanumeric information. The sample must be delivered to the laboratory as soon as possible. During transport must not lead to deterioration of the sample (eg. Fresh samples should be kept refrigerated). 3. Preparation of the test sample laboratory sample shall be assigned a special code that together with the date of receipt of the sample ID and the sample size is brought into the sampling protocol. Part of the product to be analyzed the ^ 2), i.e., the test sample should be separated as soon as possible. 4. the Preparation and storage of test dose of the test sample as required comminuted and mixed well in order to remove the representative test dose. The size of the test dose is determined by the analytical method and the efficiency of mixing. Methods for comminution and mixing should be recorded and should not affect the residues present in the test sample. Where necessary, the test sample is treated under special conditions, e.g. at a temperature lower than 0 ° C, minimize adverse effects. If processing could affect residues and are not available to use alternative procedures, test dose may consist of whole pieces or parts taken from whole pieces. It is probable that such a test dose, which consists of a small number of sub-samples or parts not too representative for the test sample, and therefore it is necessary that analyze and sufficient number of duplicate test doses, determine the degree of uncertainty of the mean value. To be a test dose before analysis stored, it is necessary that the manner and length of storage did not affect the amount of residue present. It repeat or confirmatory analysis as needed to remove additional test dose. 5. Criteria for checking the compliance of analysis results are obtained from one or more laboratory samples taken from the lot and received in a fit condition to perform the analysis. The results must be supported by acceptable quality control data ^ 3). If it is found that exceeds the minimum residue and residue limit (hereinafter ' MRL ') is required to confirm his identity and verified by analyzing the concentration of one or more other test doses derived from the original sample. MRL applies to the bulk sample. The lot meets the MRL, or if the result of the analysis results do not exceed the MRL. If the results for the bulk sample exceed the MRL, they must be a decision that reject a lot into account the following: a) the results obtained from one or more of the subsamples (b)) the accuracy and precision of analysis resulting from the supporting quality control data. Legend: 2) the Regulation of the European Parliament and Council Regulation (EC) no 396/2005 of 23 February 2005 on maximum residue levels of pesticides in food and feed of plant and animal origin and amending Council Directive 91/414/EEC. 3) quality control procedures for pesticide residue analysis. Document SANCO/3103/2000; Changes can be found on the Commission's website. 1) Commission Directive 2002/63/EC of 11 July 2002 laying down Community methods of sampling for the official control of pesticide residues in products of plant and animal origin, including fruit and vegetables, and repealing Directive 79/700/EEC. 2) Art. 7 and 11 of the European Parliament and Council Regulation (EC) no 882/2004 of 29 April 2004 on official controls to ensure verification of compliance with feed and food law, animal health and animal welfare. 3) Attachments Nos. 1 and 3 of the Decree No. 291/2003 Coll., He apart of administering some substances to animals whose products are intended for human consumption, and surveillance (monitoring) the presence of banned substances, residues and contaminants for which the animal products could be harmful to the health of humans, animals and their products, as amended. 4) Art. 11 of the Regulation of the European Parliament and Council Regulation (EC) no 882/2004. Commission Regulation No. 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of the feed. Commission Regulation (EC) no 121/2008 of 11 February 2008 laying down the method of analysis for determining the starch content of products used for animal feed.