On The Protection Of Experimental Animals

Original Language Title: o ochraně pokusných zvířat

Read the untranslated law here: https://portal.gov.cz/app/zakony/download?idBiblio=78589&nr=419~2F2012~20Sb.&ft=txt

419/2012 Sb.



DECREE



of 22 March. November 2012



on the protection of experimental animals



299/2014: Sb.



The Ministry of agriculture shall be determined according to § 29 para. 1 of law no 246/1992

Coll., on the protection of animals against cruelty, as amended by Act No. 162/1993 Coll.

Act No. 77/2004 Coll., Act No. 312/2008 Coll., Act No. 291/2009 Coll.

Act No. 359/2012 Coll. (hereinafter the "Act") to implement section 15b paragraph. 1, §

15 c of paragraph 1. 7, § 15 g of paragraph 1. 7, section 16a para. 1, § 17 para. 3, § 17a. 5

(a). b), c) and (d)), section 18 c of paragraph 1. 5 and § 18 g of paragraph 1. 8 of the Act:



§ 1



The subject of the edit



This Decree incorporates the relevant provisions of the European Union ^ 1) and

modifies the



and the pattern of the application form) to grant permission to use the experimental

animals and the model application form for permission to breed test

animals or to the supply of experimental animals,



(b)), the list of data that the breeder of experimental animals, the supplier

experimental animals or user of experimental animals must submit

reviewers, and the content of the written report of assessors,



c) patterns of statistical tables and instructions for filling it out,



d) model application form for approval of the project experiments



(e)) the requirements for premises and equipment, in terms of their function

and the General layout, breeding spaces, spaces for the implementation of the General and

specific experiments, operating facilities, the environment and its requirements

check as regards ventilation and temperature, lighting, noise, alarm

systems, and other requirements for equipment, premises and equipment,



f) requirements relating to the care and placement of test animals, if

This is the State of health of the animals harvested in the wild to the trials,

the location of the experimental animals, environment, design editing closed

space, feeding, watering and construction and equipment for relaxation and

sleep and the handling of laboratory animals,



g) requirements relating to the care and placement in relation to individual species

experimental animals,



h) matching criteria, factors relating to the attempt, and

examples of the various experiments included in each category of severity

on the basis of factors that relate to the type of experiment,



I) methods of killing each of the species of experimental animals,



(j)), the number of test animals when their carriage in the container.



§ 2



A model application form for granting permission to use experimental animals

and the model request form for granting permission to the breeding of experimental animals

or to the supply of experimental animals



(1) the model application form for granting permission to use the experimental

the animal is listed in annex 1 to this notice.



(2) the model application form for permission to breed test animals

or to the supply of experimental animals is given in annex 2 to this

the Decree.



§ 3



The list of data to be submitted and provide the content of the written report



(1) the keeper of experimental animals, a supplier of test animals, or the user

experimental animals, which asks for permission to breed test

animals, permission to supply of experimental animals or permission to use

experimental animals (hereinafter referred to as "the applicant"), reviewers shall submit to the



and the document authorizing the use of) buildings for breeding or delivery of experimental

animals or to conduct experiments on animals by building

law or binding opinion of the regional veterinary service in

land use, construction and kolaudačním proceedings, if requested by other

the law ^ 2)



(b)) a written statement to the competent regional veterinary administration activities

the device,



(c) the operational rules of the device)



d) technological procedures,



(e) documentation on the nature of the objects) of the device, including the address, the exact

the location, name, or other marking each space that

It is used for breeding or supply of experimental animals or in which they are carried out

experiments on experimental animals,



f) organizational chart of the device,



(g)) the list of jobs that can be performed only with a certificate of

professional competence to design experiments and experiments or projects with

certificate of professional competence to conduct experiments on experimental

animals, care of experimental animals and killing of experimental animals,

documented part of the list of employees with a statement of whether the certificate

It was issued,



h) document proving acquisition of relevant higher education

under the law on universities in the event DV

doctor.



(2) if the applicant is applying for permission to breed test animals to

the supply of experimental animals or to the use of experimental animals to the next

the period shall submit to provide permission for the previous period,

In addition to the particulars and documents referred to in paragraph 1,



and documentation)



1. breeding of experimental animals, in the case of breeders of experimental animals or

supplier of experimental animals, and



2. breeding and use of experimental animals, in the case of a user test

animals,



(b)) records for the



1. number of reared, purchased or supplied to experimental animals, if

the experimental animals or experimental animals, with vendors

accompanied by their origin and



2. the number of used, kept or purchased experimental animals, if

about user experimental animals, accompanied by their origin,



(c) the records of the number of dead) experimental animals,



(d) records of checks) the State of health of experimental animals, including

results of laboratory tests,



e) records of the supervisory authorities,



f) projects, including the attempts of documents that have not been approved by the professional

by the Commission for ensuring the welfare of experimental animals or

the national authority responsible for the approval of projects, experiments, if this is

about user experimental animals,



g) documentation on the experimental animals used in relation to the

projects that have taken place from the attempts of the previous assessment, assessors,

If this is about users of experimental animals,



h) information provided to record the animals to the competent authority

for the approval of projects, experiments, if this is about user test

animals,



I) changes to the information of the device or breeder of experimental animals, the vendor

experimental animals or user of experimental animals.



(3) a written opinion in addition to the requirements laid down by the administrative

regulations and requirements set out in section 15b paragraph. 4 of the Act, in particular,



and) the name or names, and surname of the tasters, the number of the certificate of

professional competence to design experiments and projects and experiments

assessor,



(b)) a statement whether the applicant has submitted all the information and documents referred to in

paragraph 1 or in paragraph 2,



(c) the assessment of the dossier submitted), in particular the identified deficiencies and

deficiencies,



(d) assessment of the spatial capacity) and technical equipment

the space in which they are kept supplied and used experimental animals,

in particular, the detected faults and shortcomings,



(e) measures for the immediate) recommended the implementation before a decision to

to grant permissions to the breeding of experimental animals, for supply of experimental animals

or to the use of experimental animals and long-term measures recommended

the character, along with a deadline for their implementation,



f) opinion of the assessors, whether the applicant meets or does not meet the requirements of the

on the breeding of experimental animals, the supply of experimental animals or to use

experimental animals provided for by law and by the Decree; does not satisfy the

the applicant also putting these requirements, shortcomings that are in violation of the

by law or by this Decree,



(g) the name of the Government Department responsible) to approve projects experiments

pursuant to section 23 of the Act, if it is a user of experimental animals,



h) Declaration of the breeders of experimental animals, a vendor of experimental animals

or users of experimental animals, it is with the content of his report, the familiar,

date and signatures of the assessors and the applicant.



§ 4



Patterns of statistical tables, instructions for filling it out, and for more information

that must include a summary report on the activities of the device



Patterns of statistical tables and instructions for filling it out are listed in

Annex No. 3 to this notice.



§ 5



Pattern approval request form project experiments



Pattern approval request form project experiments is given in annex

No 4 to this notice.



§ 6



The requirements for facilities, equipment, and environment requirements

his control and other requirements on the equipment, premises and equipment



(1) the requirements for premises and equipment, in terms of their

the function and the General layout, breeding spaces, spaces for the implementation

General and specific experiments, operating facilities, requirements for the

environment and its control in terms of ventilation, lighting, temperature and

noise, alarm systems, are listed in annex 6 to this Ordinance.



(2) the requirements for the premises, their size and facilities in relation to the

individual species of experimental animals and requirements relating to the care and

the location in relation to individual species of experimental animals are listed in the

Appendix 7 to this Decree.



§ 7



Requirements relating to the care and placement of experimental animals



Requirements relating to the care and placement of test animals, if it is

the health status of the animals harvested in the wild to the trials,


the location of the experimental animals, environment, design editing closed

space, feeding, watering and construction and equipment for relaxation and

sleep and the handling of laboratory animals are listed in annex 8 to

This Decree.



§ 8



Matching criteria attempts to various categories of severity,

factors and examples of different experiments listed in each category

severity based on factors



Matching criteria, factors relating to the attempt, and

examples of the various experiments included in each category of severity

on the basis of factors that relate to a given species, are listed

in Appendix 9 to this Decree.



§ 9



Methods of killing each of the species of experimental animals



Methods of killing each of the species of experimental animals are listed in the

Appendix 10 to this Decree.



§ 10



The number of test animals when their carriage in the container



The number of test animals when their carriage in the container is listed in

Appendix 11 to this Decree.



§ 11



Transitional provision



Until 31 December 2006. December 2016 may breeder of experimental animals, the supplier

experimental animals and the user of experimental animals in spaces

inside the device, that are permanently intended for farming or the use of

experimental animals, breeding size containers or cages, the spatial

the arrangement of the boxes or stalls for selected species of experimental animals by

the existing legislation.



§ 12



Regulation (EEC)



Shall be repealed:



1. Decree No. 207/2004 Coll., on the protection, breeding and use of experimental

animals.



2. Decree No. 39/2009 Coll., amending Decree No. 207/2004 Coll.

the protection, breeding and use of experimental animals.



3. Decree No. 128/2010 Coll. on competence for identification of dogs by tattooing.



section 13 of the



The effectiveness of the



This Decree shall enter into force on 1 January 2000. January 1, 2013.



Minister:



Ing. Sarah b in r.



Annex 1



A model application form for granting permission to use experimental animals



Annex 2



Model request form for granting permission to the breeding of experimental animals

or to the supply of experimental animals



Annex 3



Patterns of statistical tables and instructions for filling it out



Annex 4



Pattern approval request form project experiments



Annex 5



cancelled



Annex 6



Requirements for premises and equipment, in terms of their function and

General layout, breeding spaces, spaces for the implementation of the General and

specific experiments, operating facilities, the environment and its requirements

check as regards ventilation and temperature, lighting, noise, alarm

systems



(1) the keeper of experimental animals, a supplier of test animals, and the user

experimental animals shall ensure that the premises comply with the following

requirements and to be carried out the following activities, if it is a



and function and general arrangement), where



1. all installations and equipment must be so designed as to species

experimental animals, that are placed in them, provide an environment

that takes into account their physiological and behavioural needs. In addition, the

must be dealt with and managed so as to ensure, prior to entering

unauthorized persons, persons who have obvious symptoms of the disease

portable on experimental animals, and against the intrusion of other animals or leakage

experimental animals here located,



2. the devices must be put in the active maintenance programme to ensure the

prevent and remedy all the damages to buildings or equipment,



3. the device must be available and facilities to enable the newly placed

the obtained experimental animals in isolation until their health is not detected

status, and assessed and minimized any risk to your device

living the experimental animals,



4. must be available to separate areas for the location of sick or

injured animals



(b) where the premises)



1. the devices must be a regular and efficient cleaning program

space and maintained in satisfactory hygienic standards



2. walls and floors in the premises shall be provided with a particularly durable

the surface, which is not subject to heavy wear of the experimental animals or

wear of the cleaning work. The material must be for experimental animals

harmless and may not hurt. Tools and equipment must be

particularly protected so that experimental animals could not harm or

They injure itself,



3. nesnášenlivé species of experimental animals, such as the predator and its

potential prey, or experimental animals requiring different life

the conditions must not be placed in the same room and in the case of

predator and prey must be at such a distance to each other

seen, felt or heard,



(c)) for the implementation of space the General and specific experiments, where



1. the laboratory shall be provided with facilities for the implementation of the

simple diagnostic tests, post-mortem examinations or for

sampling, which will be subjected to more thorough laboratory elsewhere

in tests. There, where these operations are carried out, must be available

spaces for the implementation of General and specific experiments,



2. must be available one or more properly equipped rooms and

device for postoperative recovery, if it is necessary to perform a surgical

interventions under sterile conditions,



d) operating areas where



1. storage areas must be handled, used and maintained to

ensure the quality of feed and litter. These premises must be within the

options are protected against insects and other pests. Other materials that

could be contaminated or could pose a risk to

experimental animals or employees, shall be stored separately,



2. There must be space for cleaning and washing so large that to them was

can place a device for cleaning and disinfection of used equipment.

The cleaning process must be carried out so as to clean equipment when handling

without coming into contact with the polluted and freshly cleaned equipment so

protect from contamination,



3. the device must ensure hygienic storage and safe disposal

the bodies of dead animals and animal waste.



(2) environment and its control must meet the following requirements

If this is a



ventilation and temperature), where



1. insulation, heating and ventilation of the room must provide breeding,

that air circulation, dust and gas concentrations are kept in

limits which are not harmful to located the experimental animals,



2. the temperature and relative humidity in the breeding areas must be

adapted to the species of experimental animals and wagons age groups.

The temperature must be measured and logged daily,



3. experimental animals shall not be kept in climatic runs

conditions, which would raise the fear in them,



b) lighting, where



1. in the case that natural daylight does not provide the proper rhythm of the day

and night, this rhythm must be secured with a view to artificial lighting

to satisfy the biological needs of experimental animals and at the same time to ensure

satisfactory working conditions,



2. lighting must meet the needs of the implementation of animal husbandry and

tours of the experimental animals,



3. must be ensured by regular fotoperiodicita and illumination intensity

appropriate for the species of experimental animals,



4. when breeding albino experimental animals must be lighting

adapted to the sensitivity of the test animals to light,



c) noise, where



1. noise levels including ultrasound may not have an adverse effect on good

the welfare of experimental animals,



2. the device must have alarm systems that are set to the frequency

outside the zone, on which the experimental animals are sensitive; in doing so, but it must be

audible to the human ear,



3. premises must be where it is necessary in the interests of good

the living conditions of experimental animals, equipped with noise insulation and

sound-absorbing materials



d) alarm systems, where



1. equipment with electrical or mechanical equipment for the control and

the protection of the environment must have a spare system to ensure basic

services and the emergency lighting and the operation of alarm systems themselves,



2. heating and ventilation systems must be equipped with monitoring devices

and alarm devices,



3. clear instructions for emergency situations shall be visibly placed.



Annex 7



Requirements for the premises, their size and facilities in relation to the

individual species of experimental animals and requirements relating to the care and

the location in relation to individual species of experimental animals



1. Mice, rats, gerbils, hamsters and Guinea Pigs



In the following tables for mice, rats, gerbils, hamsters, and

Guinea Pigs are "enclosure height" means the vertical distance

the space between the floor and ceiling, the height of the space should have

For more than 50% of its minimum floor area before adding devices

to modify the environment. When planning experiments must be taken into account the possible

the growth of experimental animals in order to ensure sufficient space for them (as

It is mentioned in detail in tables 1.1 to 1.5) throughout the study.



Table 1.1

------------------------------------------------------------- ------------------------------------------------

The mouse


Physical minimum area floor area minimum height

the weight of the confined space per animal enclosure

(g) (cm2) (cm2) (cm)

------------------------------------------------------------- ------------------------------------------------

Available in the facility up to 20 330 60 12

and during experiments

from 20 to 25 330 70 12

from 25 to 30 330 80 12

over 30 330 100 12



Reproduction 330 12

For a monogamous pair

(outbrední/inbred)

or three of a kind

(inbred). For

each additional female

and litter add

180 cm2.

------------------------------------------------------------- ------------------------------------------------

Breeding animals ^ 1) less than 20 950 40 12

------------------------------------------------------------- ------------------------------------------------

Area closed

space

950 cm²



The area contained less than 20 1500 30 12

space

1500 cm2

------------------------------------------------------------- ------------------------------------------------



Note:



1) Mouse after weaning can behave at these higher stocking densities in

the short period after weaning to use in experiments, provided that

the animals are placed in large enclosed spaces with a reasonably

the modified environment and location of these conditions do not adversely affect the

animal welfare, for example, do not cause increased aggression,

morbidity and mortality, the development of stereotypií and other behavioral disorders, weight loss

weight or other disruption of physiological functions or behaviour in the

as a result of stress.



Table 1.2

------------------------------------------------------------- ------------------------------------------------

The rats

Body weight minimum area floor area minimum height

(g) an enclosed space per animal enclosure

(cm2) (cm2) (cm)

------------------------------------------------------------- ------------------------------------------------

Available up to 200 800 200 18

in the device, and during

attempts to "^ 1")

from 200 to 300 800 250 18

from 300 to 400 800 350 18

from 400 to 600 800 450 18

over 600 1500 600 18



Reproduction of 800 18

Mother and litter. For

each additional adult

animal located

on a permanent basis to a closed

space is added

400 cm2.

------------------------------------------------------------- ------------------------------------------------

Breeding animals ^ 2) up to 50 1500 100 18

------------------------------------------------------------- ------------------------------------------------

Area closed

space

1500 cm2

from 50 to 100 1500 125 18

from 100 to 150 1500 150 18

from 150 to 200 1500 175 18

------------------------------------------------------------- ------------------------------------------------

Breeding animals ^ 3) up to 100 2500 100 18

------------------------------------------------------------- ------------------------------------------------

Area closed

space



2500 cm2

from 100 to 150 2500 125 18

from 150 to 200 2500 150 18

------------------------------------------------------------- ------------------------------------------------



Notes:



1) in the implementation of long-term studies, if at the end of these studies

the space allocated to each individual experimental animal less than the

mentioned above, is a priority of maintaining a socially stable structures.



2) Rats after weaning can behave at these higher stocking densities

in the short period after weaning to use in experiments, provided that

experimental animals are placed in large enclosed spaces with

appropriately modified by the environment and these terms do not have location

an adverse effect on the welfare of experimental animals, for example,

do not cause increased aggression, morbidity and mortality, the development of

stereotypií and other behavioral disorders, weight loss or other breach

physiological function or behavior as a result of stress.



3) Rats after weaning can behave at these higher stocking densities

in the short period after weaning to use in experiments, provided that

experimental animals are placed in large enclosed spaces with

appropriately modified by the environment and these terms do not have location

an adverse effect on the welfare of experimental animals, for example,

do not cause increased aggression, morbidity and mortality, the development of

stereotypií and other behavioral disorders, weight loss or other breach

physiological function or behavior as a result of stress.



Table 1.3

------------------------------------------------------------- ------------------------------------------------

Gerbils

Minimum Floor area weight minimum height

(g) confined space confined space area

one animal (cm2) (cm)

(cm2)

------------------------------------------------------------- ------------------------------------------------

Available up to 40 1200 150 18

in the device

and during experiments

over 40 1200 250 18



Reproduction 1200 18

Monogamous pair or

Trio

with a child.

------------------------------------------------------------- ------------------------------------------------



Table 1.4

------------------------------------------------------------- ------------------------------------------------

Hamsters

Minimum Floor area weight minimum height

(g) a closed confined space area

space per animal (cm)

(cm2) (cm2)

------------------------------------------------------------- ------------------------------------------------

Available up to 60 800 150 14

in the device

and during experiments

from 60 to 100 800 200 14

over 100 800 250 14

Reproduction 800 14

Mother or

monogamous couple

with a litter of pups.



Breeding less than 60 1500 100 14

animals ^ 1)

------------------------------------------------------------- ------------------------------------------------



Note:



1) Hamsters after weaning can behave at these higher stocking densities

in the short period after weaning to use in experiments, provided that

experimental animals are placed in large enclosed spaces with

appropriately modified by the environment and these terms do not have location

an adverse effect on the welfare of experimental animals, for example,

do not cause increased aggression, morbidity and mortality, the development of

stereotypií and other behavioral disorders, weight loss or other breach

physiological function or behavior as a result of stress.



Table 1.5

------------------------------------------------------------- ------------------------------------------------

Guinea Pigs

Minimum Floor area weight minimum height

(g) a closed confined space area

space per animal (cm)

(cm2) (cm2)

------------------------------------------------------------- ------------------------------------------------

Available up to 200 1800 200 23

in the device

and during experiments

from 200 to 300 1800 350 23

from 300 to 450 1800 500 23

from 450 to 700 2500 700 23

over 700 2500 900 23



Reproduction 2500 23

Pair with litter

the offspring. For

each additional

breeding female is

add 1000 cm2.

------------------------------------------------------------- ------------------------------------------------



2. Rabbits



During agricultural research, in which the aim of the project requires that attempts

experimental animals were kept under similar conditions as the economic

animals kept for commercial purposes, their breeding, at least

comply with the standards referred to in Act No. 246/1992 Coll., on the protection of animals against

cruelty, as amended, and Act No. 166/1999 Coll.

veterinary care and amending certain related laws (health

Act), as amended.



In a confined space must be ensured by the elevated platform. This raised

the platform shall enable to experimental animals to lie and sit and easily under the


hide it and does not cover more than 40% of the floor area. If from

scientific or health reasons cannot be elevated platform used

must be a closed space for rabbits about 33% larger for one rabbit

and about 60% larger for two rabbits. If the raised platform used for

rabbits under ten weeks, its dimensions must be at least 55 cm to 25

cm and must be so high from the floor, so that it could test animals

to take advantage of.



Table 2.1



Rabbits older than 10 weeks



Table 2.1 applies to cages and pens. For every 3rd,

the fourth, fifth and sixth rabbit adds the additional area at least

3000 cm2 and for each additional rabbit area at least 2500 cm2.

------------------------------------------------------------- ---------------------

Terminal body Minimum floor area for one minimum height

weight or two socially compatible animals (cm)

(kg) (cm2)

------------------------------------------------------------- ---------------------

less than 3 3500 45

from 3 to 5 4200 45

over 5 5400 60

------------------------------------------------------------- ---------------------



Table 2.2

Sanjay and litter

------------------------------------------------------------- ---------------------

Weight minimum area Additional Sanjay minimum height

(kg) enclosure space (cm)

(cm2) nest boxes

(cm2)

------------------------------------------------------------- ---------------------

less than 3 3500 1000 45

from 3 to 5 4200 1200 45

over 5 5400 1400 60

------------------------------------------------------------- ---------------------



Table 2.3



Rabbits under ten weeks



Table 2.3 shall apply to the cages and pens.

------------------------------------------------------------- ---------------------

The age of minimum area the minimum minimum height

enclosure floor (cm)

the area (cm2)

one animal

(cm2)

------------------------------------------------------------- ---------------------

up to 7 weeks after 4000 800 40

unset

from 7 to 10 weeks of 4000 1200 40

------------------------------------------------------------- ---------------------



Table 2.4



Rabbits: optimal dimensions of a raised platform in a closed compartment of

the dimensions given in table 2.1.

------------------------------------------------------------- ---------------------

Age (weeks) the final body weight, Optimal, optimal height from floor

(kg) size of the confined space

(cm x cm) (cm)

------------------------------------------------------------- ---------------------

over less than 10 3 55 × 25 25

from 3 to 5 55 × 30 25

over 5 60 × 35 30

------------------------------------------------------------- ---------------------



3. The Cat



Cats must not be placed individually on more than 24 hours without

breaks. Cats that are against other cats showing up repeatedly

aggressively, is placed separately only if it is not possible to find a

of the individual, which can withstand. Social stress for all individuals placed

in pairs or in groups of monitors at least once a week. Females with

koťaty younger than four weeks or the last two weeks of pregnancy

they can be placed separately.



Table 3



Cats



The minimum space for the possession of breeding females and litter is a space reserved for the

one cat that is zoomed so that the chicks into four

months after birth relocated to premises meeting the requirements on

area of adult animals.



The feeding area and the area for the feces must be positioned

at least 0.5 m and these areas must not be confused.

------------------------------------------------------------- -----------

^ 1) floor Cornice Height

(m2) (m2) (m)

------------------------------------------------------------- -----------

Minimum for one 1.5 0.5 2

adult animal



Additional space for 0.75 0.25 —

for each additional animal

------------------------------------------------------------- -----------



Note:



1) floor area without ledges.



4. Dogs



Animals should have, where possible, outdoor enclosures. Dogs must not be placed

individually on more than four hours without a break.



Indoor enclosed space shall occupy at least 50% of the minimum space

reserved for dogs, which is shown in table 4.1.



The parameters below are based on the requirements of the allocated space for

bígly, but for the giant breeds, such as Saint Bernards or Irish

vlkodavy, spaces must be reserved considerably larger than premises specified

in table 4.1. For other breeds, than are laboratory bíglové, must be

the allocated space, consulted with veterinary staff.



Table 4.1



Dogs



Space for each of the dogs placed in pairs or in a group may be in

the course of applied experiments limited to half of the total reserved

space (2 m2 for a dog under 20 kg, 4 m² for a dog over 20 kg), as provided for in the law

or this Ordinance, if this is important for the scientific Department of the animal

purposes. Period during which the space is limited in this way, the dog must not exceed 4

hours without a break.



A nursing female dog and the young must be assigned to the same space as the

by the same weight. Birthing pen has to be designed so that it can

bitch from the puppies away to another part of the space or on elevated

rig.

----------------------------------------------------- -----------------------------------------

Minimum weight the minimum area for each additional minimum height

(kg) contained floor area animal extension (m)

room for one or two at least

(m) animals (m2)

(m2)

----------------------------------------------------- -----------------------------------------

up to 20 4 4 2 2



over 20 8 8 4 2

----------------------------------------------------- -----------------------------------------



Table 4.2



Dogs-weaned puppies

---------------------------------------- ----------------------------------------

The weight of the dog the minimum area the minimum floor minimum height

(kg) enclosure on one area (m)

animal (m2)

(m2)

---------------------------------------- ----------------------------------------

up to 0.5 5 4 2

from 5 to 10 4 2 1.0

from 10 to 15 4 1.5 2

from 15 to 20 4 2 2

nad 20 8 4 2

---------------------------------------- ----------------------------------------



5. Ferrets



Table 5

-------------------------------------- ---------------------------------------------

Ferrets

The minimum area of a minimum floor height is at least

confined space area per one (cm)

animal (cm2)

cm2)

-------------------------------------- ---------------------------------------------

animals up to 600 g 4500 1500 50

animals over 600 g 4500 3000 50

adult males 6000 6000 50

ferret with 5400 5400 50

-------------------------------------- ---------------------------------------------



6. Subhumánní primates



Young subhumánní primates from the mother may separate the age at

six to twelve months, depending on individual species. The environment must

subhumánním're primates make it possible to carry out a complete program daily

activities. Enclosed space must allow in a large well tolerated subhumánním

extent possible, satisfy their Ethological needs and provide them with a sense of

safe and sufficiently diverse environments in which animals can run,

to walk, climb and jump.



Table 6.1

-------------------------------------- ------------------------------------------------

Marmosets and tamaríni



Minimum floor area the minimum volume of minimum height

enclosed space for a single space on ^ 1) every enclosed space

or two animals and offspring to another animal (m) ^ 2)

old age five months earlier of five

(m2) months

(m3)

-------------------------------------- ------------------------------------------------

Marmosets 0.5 0.2 1.5

Tamaríni 1.5 0.2 1.5

-------------------------------------- ------------------------------------------------



In the case of kosmanů and tamarínů with the Cubs from the mother separated by the

the age of eight months.



Notes:



1) animals may be housed individually only in exceptional circumstances.



2) the ceiling of the enclosure shall be at least 1.8 m above the floor.



Table 6.2

------------------------------------------------------

Kotulové




Minimum Minimum minimum volume

floor area of the space on each height

for one ^ 1) or another animal older closed-end

two animals six months of space

(m2) (m3) (m)

-------------------------------------- ---------------

2.0 0.5 1.8

-------------------------------------- ---------------



In the case of squirrel with the Cubs from the mother may separate the age at

six months.



Note:



1) animals may be housed individually only in exceptional circumstances.



Table 6.3

--------------------------------------------------- -------------------------------

Crab-eating monkeys, and ^ 1)



Minimum Minimum Minimum Minimum area

a closed volume volume height

the space of the enclosure to the contained

(m2) of space per animal space

(m3) (m3) (m)

--------------------------------------------------- -------------------------------

Animals less than three 2.0 3.6 1.0 1.8

years ^ 2)

Animals older than three 2.0 3.6 1.8 1.8

years ^ 3)

Animals which are kept for 3.5 2.0

breeding purposes ^ 4)

--------------------------------------------------- -------------------------------



In the case of macaques and marmosets are the Cubs from the mother may separate the first

at the age of eight months.



Notes:



1) animals may be housed individually only in exceptional circumstances.



2) in a confined space with minimum dimensions may be placed

no more than three animals.



3) in a confined space with minimum dimensions may be placed

not more than two animals.



4) is not required in the breeding populations of the additional area/room for

the Cubs within two years along with his mother.



Table 6.4

------------------------------------------------------- ------------------------------------

Baboons ^ 1)



Minimum Minimum Minimum Minimum volume

the volume of space in one area height

closed closed zvířeuzavřeného

compartment (m3)

(m2) (m3) (m)

------------------------------------------------------- ------------------------------------

Animals less than four years ^ 2) 4.0 7.2 3.0 1.8

Animals over four years ^ 3) 7.0 12.6 6.0 1.8

Animals held for breeding 12.0 2.0

the purpose of the ^ 4)

------------------------------------------------------- ------------------------------------



In the case of the baboons, the Cubs from the mother may separate the age at

eight months.



Notes:



1) animals may be housed individually only in exceptional circumstances.



2) in a confined space with minimum dimensions may be placed

not more than two animals.



3) in a confined space with minimum dimensions may be placed

not more than two animals.



4) is not required in the breeding populations of the additional area/room for

the Cubs within two years along with his mother.



7. Farm animals



During agricultural research, in which the aim of the project requires that attempts

experimental animals were kept under similar conditions as the economic

animals kept for commercial purposes, their breeding, at least

comply with the standards referred to in Act No. 246/1992 Coll., on the protection of animals against

cruelty, as amended, and Act No. 166/1999 Coll.

veterinary care and amending certain related laws (health

Act), as amended.



Table 7.1

------------------------------------------------------- ------------------------------------

Bovine animals



Body weight Minimum Minimum space at the trough the space at the trough when

(kg) area of the floor when you adlibitním a restrictive

the closed area to feed the bezrohého feeding bezrohého

space per animal of bovine cattle

(m2) (m2/animal) (m/animal) (m/animal)

------------------------------------------------------- ------------------------------------

up to 100 2.50 2.30 0.10 0.30

from 100 to 200 4.25 3.40 0.15 0.50

from 200 to 400 6.00 4.80 0.18 0.60

from 400 to 600 9.00 7.50 0.21 0.70

from 600 to 800 11.00 8.75 0.24 0.80

over 800 16.00 10.00 0.30 1.00

------------------------------------------------------- ------------------------------------



Table 7.2

-------------------------------------------------------------- ---------------------------------

Ovine and caprine animals



Minimum Minimum Minimum body surface area of the space at the trough

the weight of the contained floor area height in the gutter when you

(kg) of space per animal desk adlibitním a restrictive

(m2) (m2/animal) (m) feeding

(m/animal) (m/animal)

-------------------------------------------------------------- ---------------------------------

less than 20 1.0 0.7 1.0 0.10 0.25

from 20 to 35 1.5 1.0 1.2 0.10 0.30

from 35 to 60 2.0 1.5 1.2 0.12 0.40

over 60 3.0 1.8 1.5 0.12 0.50

-------------------------------------------------------------- ---------------------------------



Table 7.3

-------------------------------------------------------------- ---------------------------------

Pigs and miniprasata



Live weight Minimum Minimum minimum area of a closed area for encampment

(kg) ^ 1) floor area per animal

(2) (vtermoneutrálních area

one animal conditions)

(m2/animal) (m2/animal)

-------------------------------------------------------------- ---------------------------------

up to 5 2.0 0.20 0.10

from 5 to 10 2.0 0.25 0.11

from 10 to 20 2.0 0.35 0.18

from 20 to 30 2.0 0.50 0.24

from 30 to 50 2.0 0.70 0.33

from 50 to 70 3.0 0.80 0.41

from 70 to 100 3.0 1.00 0.53

from 100 to 150 4.0 1.35 0.70

over 150 5.0 2.50 0.95

Adult males (pig 7.5 1.30

Home)

-------------------------------------------------------------- ---------------------------------



Note:



1) Pigs may be for veterinary or experimental reasons for shorter

the period closed in smaller spaces, created such as přepažením

the main space, for example, the individual is required.



Table 7.4.



The equidae



The shortest side is at least half larger than the height of the animal in the

the withers. The height of indoor enclosed space allows the animals to

completely straightened.

-------------------------------------------------------------- ---------------------------------

Height at the Withers, the minimum floor area per animal, the minimum height

(m) (m2/animal) enclosure

(m)

-------------------------------------------- --------------------------

For each animal for each Birth

held by the animal held box/Mare

individually or in groups with foal

Group of four

the number of animals or more

animals

-------------------------------------------------------------- ---------------------------------

from 1.00 to 3.00 16 1.40 9.0 6.0

from 1.40 to 1.60 12.0 9.0 20 3.00

over 1.60 16.0 (2 x height 20 3.00

at the withers) ^ 1)

-------------------------------------------------------------- ---------------------------------



Note:



1) to ensure there is sufficient space must surround the conditions for

each experimental animal be based on its height at the withers.



8. The birds



During agricultural research, in which the aim of the project requires that attempts

experimental animals were kept under similar conditions as the economic

animals kept for commercial purposes, their breeding, at least

comply with the standards referred to in Act No. 246/1992 Coll., on the protection of animals against

cruelty, as amended, and Act No. 166/1999 Coll.

veterinary care and amending certain related laws (health

Act), as amended.



Table 8.1



Chickens



If not for scientific reasons, the following minimum dimensions closed

space to make sure they justify the time of conclusion of the birds in a limited space

the person performing the experiment in consultation with veterinary staff. Under these

circumstances, birds can be housed in smaller confined areas with

the appropriate editing environment and a minimum floor area of 0.75 m2.

-------------------------------------------------------------- ---------------------------------

Body weight Minimum Minimum Minimum minimum area

(g) confined space area at the height of the feeder on the one

(m2) per bird (cm) bird

(m2) (cm)


-------------------------------------------------------------- ---------------------------------

up to 200 1.00 0.025 30 3

from 200 to 300 1.00 0.03 30 3

from 300 to 600 1.00 0.05 40 7

from 600 to 1200 2.00 0.09 50 15

from 1200 to 1800 2.00 0.11 75 15

from 1800 to 2400 2.00 0.13 75 15

over 2400 2.00 0.21 75 15

-------------------------------------------------------------- ---------------------------------



Table 8.2



Turkeys



All parties in the confined space for experimental animals must be long

at least 1.5 m. If not for scientific reasons, the following minimum dimensions

make sure they justify the time of conclusion of the birds in a confined space, the person

conducting the trial in consultation with veterinary staff. Under these

circumstances, birds can be housed in smaller areas with appropriate

by modifying the environment and minimum floor area of 0.75 m2 and a minimum

the height of 50 cm for birds weighing up to 0.6 kg, 75 cm for birds to

weight of 4 kg, and 100 cm for birds weighing more than 4 kg. These

spaces may be used for placement of small groups of birds in accordance with the

the spatial conditions listed in table 8.2.

-------------------------------------------------------------- ---------------------------------

Physical minimum area the minimum minimum height minimum length feeders

the mass of a closed space (cm) area per bird

(kg) (m) a (cm)

Bird

(m2)

-------------------------------------------------------------- ---------------------------------

up to 0.3 2.00 0.13 50 3

from 0.3 to 0.6 2.00 0.17 50 7

from 0.6 to 1 100 15 2.00 0.30

from 1 to 4 2.00 0.35 100 15

from 4 to 8 100 15 2.00 0.40

from 8 to 12 2.00 0.50 150 20

from 12 to 16 2.00 0.55 150 20

from 16 to 20 150 20 2.00 0.60

over 20 150 20 3.00 1.00

-------------------------------------------------------------- ---------------------------------



Table 8.3.

-------------------------------------------------------------- ---------------------------------

Quail



Body on the minimum area of 1 Area to minimum minimum length

weight area bird feeders on the height of each one

(g) the closed positioned next bird (cm) bird

space in a pair of under (cm)

(m2) (m2) location

-------------------------------------------------------------- ---------------------------------

up to 150 1.00 0.5 0.10 20 4

over 150 1.00 0.6 0.15 30 4

-------------------------------------------------------------- ---------------------------------



Table 5.2



Ducks and geese



If not for scientific reasons, the following minimum dimensions closed

space must the time of conclusion of the birds in a limited space

justified by the person making the attempt, in consultation with veterinary staff.

In such circumstances, birds can be housed in smaller enclosed

areas with appropriate editing environment and minimum floor space

0.75 m2. These spaces can be used for placement of small groups

birds in accordance with the spatial conditions listed in table 8.4.

-------------------------------------------------------------- ---------------------------------

Body weight minimum area Area on the minimum minimum length

(g) an enclosed space per bird feeder height on one

(m2) (m2) ^ 1) (cm) bird

(cm)

-------------------------------------------------------------- ---------------------------------

ducks

up to 300 2.00 0.10 50 10

from 300 to 1200 ^ 2) 2.00 0.20 200 10

over 1200 to 3500 2.00 0.25 200 15

over 3500 2.00 0.50 200 15

the geese

up to 500 2.00 0.20 200 10

from 500 to 2000 2.00 0.33 200 15

over 2000 2.00 0.50 200 15

-------------------------------------------------------------- ---------------------------------



Notes:



1) Includes a water tank with a minimum area of 0.5 m2 on 2 m2 closed

space with a minimum depth of 30 cm. water tank can account for up to 50%

the minimum area enclosed space.



2) Immature birds can be kept in enclosed areas with minimal

height 75 cm.



Table 8.5



Ducks and geese: minimum size of water tank ^ 1)

----------------------------------------------------

Area Depth

(m2) (cm)

----------------------------------------------------

ducks 0.5 30

geese 0.5 from 10 to 30

----------------------------------------------------



Note:



1) water tank Dimensions apply to enclosed space covering an area of 2 m2.

The water tank can be up to 50% of the minimum area enclosed space.



Table 8.6.



Pigeons



Enclosed spaces must be rather long and narrow (for example, 2 m x 1 m)

than square to allow birds short flights.

---------------------------------------------------- -----------------------------------------

The minimum area of a minimum group size of Minimum Minimumlength perch

enclosure height length per bird feeders

(m2) (cm) (cm)

Bird

(cm)

---------------------------------------------------- -----------------------------------------

up to 6 2 200 5 30

from 7 to 12 3 200 5 30

for each 0.15 5 30

the next bird

over 12

---------------------------------------------------- -----------------------------------------



Table 5.4



Colorful Zebřičky



Enclosed spaces must be rather long and narrow (for example, 2 m x 1 m)

than square to allow birds short flights. Breeding research

the steam can be put in small venues with appropriate editing environment

minimum floor area of 0.5 m2 and a minimum height of 40 cm. Length of time

the conclusion of the birds in a confined space must justify the person performing the

the attempt, in consultation with veterinary staff.

---------------------------------------------------- -----------------------------------------

Group size the minimum area of the closed-end minimum height of Minimálnípočet feed

area (cm)

(m2)

---------------------------------------------------- -----------------------------------------

up to 6 100 2 1.0

from 7 to 12 1.5 200 2

from 13 to 20 200 3 2.0

for each additional 0.05 1 6 birds

the bird above 20

---------------------------------------------------- -----------------------------------------



9. the Amphibians



Table 9.1

---------------------------------------------------- -----------------------------------------

Water ocasatí



Body length ^ 1) minimum area of water minimum area the minimum depth of water

(cm) of the surface of the water surface (cm)

(cm2) for each additional

an animal in the

under

the location of the

(cm2)

---------------------------------------------------- -----------------------------------------

up to 10262.5 50 13

from 10 to 15 525 110 13

from 15 to 20 875 200 15

from 20 to 30 1837.5 440 15

over 30 3150 800 20

---------------------------------------------------- -----------------------------------------



Note:



1) measured from the muzzle after the cloaca.



Table 5.7

---------------------------------------------------- -----------------------------------------

Water frogs ^ 1)



Body length ^ 2) minimum area of water minimum area the minimum depth of water

(cm) of the surface of the water surface (cm)

(cm2) for each additional

an animal in the

under

the location of the

(cm2)

---------------------------------------------------- -----------------------------------------

less than 6 160 40 6

from 6 to 9 300 75 8

from 9 to 12 600 150 10

over 12 920 230 12.5

---------------------------------------------------- -----------------------------------------



Notes:



1) these terms and conditions apply to tanks in the breeding areas (i.e., when

breeding) rather than the tanks for natural mating and superovulaci because of

performance, when the individual tanks require a smaller size.

Space requirements are established for adults in those

categories of size; young individuals or tadpoles are excluded, or

they are proportionally adjusted to the dimensions.



2) measured from the muzzle after the cloaca.



Table 9.3


---------------------------------------------------- -----------------------------------------

Aquatic frogs



Body length ^ 1) Minimum Minimum minimum height of the area the minimum depth of water

(cm) closed closed area (cm)

space ^ 2) ^ 3 each space)

(cm2) animal in a (cm)

under

the location of the

(cm2)

---------------------------------------------------- -----------------------------------------

up to 1500 200 20 10 5.0

from 5.0 to 7.5 3500 500 30 10

over 7.5 4000 700 30 15

---------------------------------------------------- -----------------------------------------



Notes:



1) measured from the muzzle after the cloaca.



2) one third of the terrestrial part, two-thirds of the water surface is sufficient

for diving animals.



3) measured from the floor up to the inner part of the terrestrial part of the ceiling

the terrarium; In addition, the height of the enclosure shall be adapted to the

its internal layout.



Table 5.8

---------------------------------------------------- -----------------------------------------

Predominantly terrestrial frogs



Body length ^ 1) Minimum Minimum Minimum Minimálníhloubka water area

(cm) of the closed area for height (cm)

space ^ 2) each additional closed

(cm2) an animal in the area ^ 3)

the (cm)

the location of the

(cm2)

---------------------------------------------------- -----------------------------------------

up to 1500 200 20 10 5.0

from 5.0 to 7.5 3500 500 30 10

over 7.5 4000 700 30 15

---------------------------------------------------- -----------------------------------------



Notes:



1) measured from the muzzle after the cloaca.



2) two thirds of the terrestrial part, one-third of the water surface is sufficient

for diving animals.



3) measured from the floor up to the inner part of the terrestrial part of the ceiling

the terrarium; In addition, the height of the enclosure shall be adapted to the

its internal layout.



Table 5.9

------------------------------------ -----------------------------------------------

Tree frogs



Body length ^ 1) minimum area the minimum area minimum height

(cm) enclosure "^ 2") for each additional enclosure ^ 3)

(cm2) animal in a (cm)

under

the location of the

(cm2)

------------------------------------ -----------------------------------------------

up to 3.0 900 100 30

over 1500 200 30 3.0

------------------------------------ -----------------------------------------------



Notes:



1) measured from the muzzle after the cloaca.



2) two thirds of the terrestrial part, one-third of the water surface is sufficient

for diving animals.



3) measured from the floor up to the inner part of the terrestrial part of the ceiling

the terrarium; In addition, the height of the enclosure shall be adapted to the

its internal layout.



10. Reptiles



Table 10.1

------------------------------------ -----------------------------------------------

Water turtles



Body length ^ 1) minimum area the minimum area of the minimum depth of water

(cm) of water surface, water surface (cm)

(cm2) for each additional animal in

the location of the

(cm2)

------------------------------------ -----------------------------------------------

up to 5 600 100 10

from 5 to 10 1600 300 15

from 10 to 15 3500 600 20

from 15 to 20 6000 1200 30

from 20 to 30 10000 2000 35

over 30 20000 5000 40

------------------------------------ -----------------------------------------------



Note:



1) measured in a straight line from the front after the rear edge of the carapace.



Table 10.2

------------------------------------ -----------------------------------------------

Terrestrial snakes



"^ 1" in length) minimum Minimum Minimum area height

(cm) floor area for each additional animal in uzavřenéhoprostoru ^ 2)

(cm2) the location (cm)

(cm2)

------------------------------------ -----------------------------------------------

up to 30 300 150 10

from 30 to 40 400 200 12

from 40 to 50 600 300 15

from 50 to 75 1200 600 20

over 75 2500 1200 28

------------------------------------ -----------------------------------------------



Notes:



1) measured from the muzzle after tail.



2) measured from the floor up to the inner part of the terrestrial part of the ceiling

the terrarium; In addition, the height of the enclosure shall be adapted to the

its internal layout.



11. fish



11.1 the supply and quality of water



Constantly must be ensured adequate water supply of suitable quality.

Water flow in recirculating systems or filtration in the tanks must

to be sufficient and to ensure compliance with water quality parameters on

acceptable levels. The supply water must be filtered or modified.

so that, where appropriate, substances which are removed for fish

dangerous. Parameters of water quality must be kept within an acceptable

the range, which for a given type of fish in a given stage of development, maintains the normal

activity and physiology. The water flow must be sufficient to ensure that

fish allowed to swim properly and behave as usual. Fish

You must have enough time for acclimatization and adaptation to changes

conditions as to the quality of the water.



11.2 oxygen, nitrogen compounds, pH and salinity



Oxygen concentration must match the needs of each species of fish and

the conditions in which fish are kept. If necessary, must be

ensures additional aeration of the water in the tanks. The concentration of

compounds of nitrogen must be kept at lower levels.



The pH level must be adapted to the individual species of fish and maintained

If possible, stable. Salinity must be adapted to the requirements of the species

the fish and the stage of individuals. Changes of salinity must be carried out gradually.



11.3 Temperature, lighting, noise



The temperature must be maintained within the optimal range for the species of fish and

as far as possible on a stable value. Temperature changes must be carried out

gradually. The fish must be ensured by appropriate fotoperiodicita. The levels of

noise must be kept to a minimum and the equipment causing the noise or

vibration, such as electrical generators or filter systems must

be where appropriate, separated from the tanks in which are kept

the fish.



11.4. Stocking density and diversity of the environment



The stocking density of the fish must be based on the overall needs of the fish when it comes to

environmental conditions, health status and well-being. Must be

ensure a sufficient volume of water to keep the fish could swim in the normal way,

taking into account the size, age, State of health, and how

the feeding of the fish. The environment of the fish must be suitably modified, for example,

shelters must be provided, and substrate on the bottom, except for cases where the of the

their behaviour patterns shows that do not.



11.5. Feeding and handling



The fish must be fed a diet that is suitable for them, in the appropriate

quantities and intervals. Special attention should be paid to the feeding

FRY during each transition from natural food on plastic. Handling

with the fish must be kept to a minimum.



Annex 8



Requirements relating to the care and placement of test animals, if it is

the health status of the animals harvested in the wild to the trials,

the location of the experimental animals, environment, design editing closed

space, feeding, watering and construction and equipment for relaxation and

sleep and the handling of laboratory animals



Care of experimental animals and their location shall meet the following

requirements, if it is a



and health, where)



1. in all facilities must be put in place strategies for maintaining

the State of health of experimental animals, which makes them good

living conditions and corresponds to the scientific requirements. This strategy must

include regular monitoring of the State of health of experimental animals,

microbiological checks and program plans to address health problems

experimental animals and shall define health parameters and procedures for the

the adoption of new experimental animals, and



2. tours of the experimental animals must be carried out by a person competent to

conduct experiments on experimental animals, experimental animals and care

the killing of experimental animals at least once a day. These tours must

ensure that this person finds all sick or injured in experimental

animals and shall take the appropriate measures to remedy the condition



(b)) experimental animals harvested in the wild, where



1. at the point of capture must be available for shipping containers and

means of transport adapted to the species of experimental animals for

any transport experimental animals to examinations or treatment, and



2. particular attention must be paid to habituation, quarantine, the location of the


and the breeding of experimental animals caught in the wild and caring for them and

where appropriate, the preparation of their release into the wild after the end of the

and for these situations must be provided conditions suitable for the

a kind of experimental animals,



(c)) the location of the experimental animals, in addition to the solitary by nature experimental

animal experimental animals must be placed in a socially stable

groups of individuals who are snášenliví to each other. In cases where the

permitted State authority competent to approve the projects at

the decision approving the project attempts an individual location, must be

the length of this location as much as possible and in its course must be

experimental animal maintained by Visual, auditory, olfactory, or touch

contact. Location, or the return of the experimental animals into established groups

must be carefully monitored, the avoidance of problems caused by

intolerance and not to disrupt social relationships,



(d) environment, all editing) to experimental animals must be secured

diverse enough space that they extensively will allow common

behavior. Within their environment, they must be granted a certain degree of

control and choice, in order to reduce symptoms of stress-induced behavior.

The device must apply suitable procedures for editing environment that

extend the range of potential activities of experimental animals and promote their

adapting situation, including the body of the movement, getting food, playing

and cognitive activities, according to the species of experimental animals. Editing environment

in confined spaces for experimental animals must be adapted to the

the species of experimental animals and to the individual needs of the

experimental animals. Strategy in the field of editing environment must be in

devices subject to the review and updated regularly,



e) enclosed spaces for experimental animals, shall not be produced from

materials that are harmful to the health of experimental animals. Must be

designed and constructed in such a way that the experimental animals could not injure themselves.

If they are not intended for single use, it must be of resistant

the material, which is not harmful to cleaning and disinfection. Floor closed

space must be adapted to the species of experimental animals and their

old age and to be so designed as to facilitate the removal of excreta,



f) feeding, where



1. the form, the content and the presentation of feed shall conform to the nutritional and

physiological needs of the test animal,



2. feed the experimental animals must be tasty and uncontaminated. When

selection of raw materials for animal feed and in the production, preparation and presentation of feed shall

be taken in order to minimize the risk of chemical,

physical and microbial contamination,



3. feed must be packaged, transported and stored, so that the

avoid contamination, deterioration or destruction. All hoppers,

troughs and other tools to be used for feeding must be cleaned regularly and

If necessary, sterilised



4. protect every animal must be ensured access to the food with the

adequate space to reduce competition,



g) power, where



1. with regard to the physiological needs of the species must have the experimental animals

still available to water that does not endanger the GMO-their

the State of health,



2. must be regularly inspected, maintained and proplachovány,

If automatic power systems by preventing

accidents. If they are used the cage with a fixed date, care must be taken to

minimize the risk of flooding,



3. it must be ensured that the water supplied to the aquariums and tanks was

adapted to the needs and boundaries of tolerance of each species,

Amphibians and reptiles



h) area for rest and sleep, where



1. experimental animals must be constantly available litter or

construction for sleeping, that are tailored to the needs of the species concerned

experimental animals, including materials or structures for building nests in the

breeding of experimental animals, and



2. in confined spaces for experimental animals must be according to the needs

each species of experimental animals provided a solid and comfortable area

for relaxation. All spaces for sleep must be kept clean and

dry,



I) handling, where on the device must be put in place programs to habituation and

training adapted to experimental animals, experiments, and the duration of the project

attempts.



Annex 9



Matching criteria, factors relating to the attempt, and

examples of the various experiments included in each category of severity

on the basis of factors relating to the kind you try to



(1) the assignment of severity category must



and take into account all interventions on) experimental animals or handling

them within a defined procedure



(b)) based on the most serious effects of each experimental animals

You may feel after using all relevant mitigation procedures



(c) take into account the type of attempt and) the factors referred to in paragraphs 2 and 3, all

These factors must be assessed in each individual case.



(2) the factors relating to the attempt to include



and the kind of treatment and handling) with experimental animals,



(b)) the nature of pain, suffering, distress or lasting harm of the experimental

the animal as a result of all the elements of the attempt and its intensity, duration,

the frequency and diversity of the methods used,



c) cumulative suffering within test animal,



d) guard in the speeches of the natural behavior of the test animal, including

restrictions with regard to the location and standards of care.



(3) for the purposes of final severity classification attempts must take into account and

These additional factors shall be assessed for each individual case,



and) the type of experimental animals and genotype,



b) adulthood, age and sex of the test animal,



(c)) navyklost test animal on any given attempt,



(d)) in the case of experimental animal use again, the actual

the severity of previous attempts,



e) methods used to mitigate or eliminate pain, suffering and distress

test animal, including the refinement in the location, breeding and

care,



f) terms the humane treatment of experimental animal.



(4) the Mild kinds of experiments include



and numbness) except in the case where the sole purpose of

the killing of the experimental animal,



b) pharmacokinetic studies, when the protect the animal served only one

dose and removes a limited number of blood samples, that is, the total amount of

less than 10% of the volume in circulation and it is not expected that the substance causes

detectable adverse effect,



(c)) non-invasive imaging of experimental animals, such as MRI, using

the appropriate sedatives or pain-reducing substances,



d) surface, such as a biopsy of the attempts of the ear and tail, non-surgical

subcutaneous minipump implantation and transponders,



e) using external telemetry devices that test animals

cause only slight injury or interfere with normal activities

and behavior,



(f) administration of substances), intramuscular, Intraperitoneal, subcutaneous route

through the probe and intravenously via superficial blood vessels, when

substance has on experimental animal larger than a slight impact and volume is within the

appropriate limits for the size and type of test animal,



g) induction of tumors, or spontaneous tumors, that cause no detectable

Clinical adverse effects of protect animal, such as a small, subcutaneous,

non-invasive nodules,



h) breeding genetically altered experimental animals, which are expected to

the emergence of a phenotype with mild effects,



I) feeding test animal modified food which does not meet all

the nutritional needs of the experimental animal, which, it is expected that in the course of

the study will cause mild clinical abnormalities,



j) short-term, i.e., less than 24 hours of the conclusion of the trial,

animal metabolic cage



the study, which included) is a short-term separation of test animal

from the social partners or short-term separate location of adult

rats or mice with social tendencies in cages,



l) models that expose the experimental animals harmful stimuli that are

briefly associated with slight to moderate pain, suffering or fear, and

establishing experimental animals can successfully avoid,



m) on the basis of a combination or accumulation of the following examples can be

experiments classified as mild



1. assessing body composition test animal with the help of non-invasive

methods and minimum restrictions,



2. monitoring of the ECG test animal with non-invasive methods for minimum

limitation of přivyklých experimental animals or without limitation,



3. use of external telemetry devices, which are not expected to

No injury caused by socially adapted to experimental animals or intervention

in a regular activity and the behavior of the test animal,



4. the breeding of genetically altered experimental animals, which are not expected to

the occurrence of any clinically identifiable Harmful phenotypes,



5. Add inert markers to food animals for the purpose of

tracking the passage of feces,



6. withdrawal of food adult rats for a period not exceeding 24 hours,



7. tests of an open field.




(5) medium kinds of experiments include



and the frequent use of test substances), which produces medium-intense

clinical effects of, and the collection of blood samples, which represent less than 10

% of the volume in circulation in experimental animal conscious within a few days without

filling,



b) studies to determine the extent of the effective dose or the test

chronic toxicity and carcinogenicity, which does not stop killing trial

of the animal,



c) surgical procedures in the overall numbness, and using appropriate

funds reduce the pain associated with pain after surgery, suffering

or the deterioration of the general condition of the experimental animal, in particular torakotomie,

craniotomy, laparotomy, orchidectomy, lymphadenectomy,

tyroidektomie, orthopaedic surgery with effective stabilisation and treatment

wounds, organ transplantation with effective managing rejection, surgical

the introduction of catheters or the use of biomedical devices, such as

telemetry transmitters, minipump,



(d)) ways invoking tumours or spontaneous tumors, which are expected,

that will cause moderate to intense pain or fear or moderate

interference with the normal behavior of the test animal,



e) irradiation of test animal or chemotherapy using sublethal

benefits or otherwise lethal dose, but during the reconstitution of the immune

the system. The expected adverse effects should be mild or moderate and

short-term, i.e., lasting less than 5 days,



f) breeding genetically altered experimental animals, which are expected to

the emergence of a phenotype with medium effects,



(g) the creation of a genetically modified) test animal on the basis of

surgical procedures,



(h)), thus lasting up to 5 days, the use of metabolic cages

with moderate restriction of movement of the test animal,



I) study using a modified food which does not meet all the nutritional

the needs of the test animal, which, it is expected that in the course of the study

causes the animal to protect moderate clinical abnormalities,



j) deprivation of food adult rats for a period of 48 hours,



to escape or evasion) calling the reaction in a situation where the experimental animal

the initiative cannot escape or avoid it, and when it is expected to be moderate

intense fear.



(6) Serious kinds of experiments include



a) toxicity tests, which lead to the death or which can be expected

the death of test animal or the emergence of a serious patofyziologického for status

test animal, such as acute toxicity test after the application of a single

benefits,



(b) testing machines) failure can cause a great deal of pain,

fear or death test animal, such as equipment that supports

heart activity,



(c)) to test the effectiveness of vaccines that are accompanied by the persistent deterioration of the State of

the experimental animal experimental disease leading to death, associated with the

long-term moderately intense pain, fear or suffering,



d) irradiation of test animal or chemotherapy using lethal

dose without reconstitution of the immune system or reconstitution

inducing graft,



e) ways of invoking the tumours or with the emergence of spontaneous tumors, for which

It is expected that causes a progressive fatal disease of test animal

accompanied by long-lasting moderate to intense pain, fear or

suffering, such as tumors causing cachexia, invasive bone

tumors, tumors leading to the spread of metastases and tumors, which allows

ulceration,



f) surgical and other interventions on an experimental animal in the overall

anesthesia, which are expected to cause significant or permanent medium

intensive post-operative pain, suffering or distress or serious and permanent

the deterioration of the general condition of the test animal, causing unstable

fractures or torakotomie test animal without a reasonable reduction in the

pain or trauma in order to raise multiple organ failure

test animal,



g) transplant authority, where it is expected that rejection will lead to institution

significant distress or deterioration of the general condition of the test animal,

for example, on Xeno-transplantation,



h) test animal breeding with genetic disorders, which are expected to

the experimental animal will affect serious and permanent deterioration of the general condition,

for example, Huntington's disease, muscular dystrophy, chronic

recurrent inflammation of the nerves,



and the longer the use of metabolic cages) with severe restrictions of movement

test animal,



j) electric shocks, before whom the experimental animal cannot escape, such as

in order to trigger a studied helplessness,



for the longer term social isolation) full of species of experimental animals,

for example, dogs and primates, subhumánních



l) immobilization stress in rats in order to cause stomach ulcers or

heart failure,



m) tests using forced swimming or exercise, which ends by exhaustion

the experimental animal.



Annex 10



Methods of killing each of the species of experimental animals



Requirements:



(1) where appropriate, the prior administration of sedatives.



(2) this applies only for large reptiles.



(3) the gas is allowed into the Chamber gradually. Not applicable for fruit and

neonatal rodents.



(4) it shall apply only in birds weighing less than 1 kg. Birds with

mass exceeding 250 g serves a sedative.



(5) it shall apply only in rodents, weighing less than 1 kg. Rodents with

weighing more than 150 g serves a sedative.



(6) it's the only rabbits weighing less than 1 kg. Rabbits with

weighing more than 150 g serves a sedative.



(7) it shall apply only in birds weighing less than 5 kg.



(8) it shall apply only in rodents, weighing less than 1 kg.



(9) applies only in rabbits weighing less than 5 kg.



(10) applies only in newborn individuals.



(11) applies only in birds weighing less than 250 g.



(12) it shall apply only if it is not possible using other methods.



(13) special equipment is required.



(14) It is only for pigs.



(15) only performs an experienced shooter in the field.



(16) only performs an experienced shooter in the field, if it is not possible to use

the other method.



Explanatory notes:



1) if the box is empty, the method of killing for a single species

experimental animals allowed.



2) if section proškrtnutá is a method of killing for a single species

experimental animals is prohibited.



3) if section a link to the request, the method of killing for

a single species of experimental animals allowed and is carried out in accordance with the

the relevant requirement.



Annex 11



The number of test animals when their carriage in the container



The number of test animals when their carriage in the container must

take into account the requirements laid down by Council Regulation (EC) No 1/2005 of the day

December 22, 2004 on the protection of animals běhme transport and souvisejícíhc

operations and amending Directives 64/432/EEC and 93/119/EC and Regulation (EC) No.

1255/97, as amended.



1) European Parliament and Council Directive 2010/63/EU of 22 December 2004. September

2010 for the protection of animals used for scientific purposes.



2) Act 166/1999 Coll., on health care and on amendments to certain

related laws (health law), as amended.