419/2012 Sb.
DECREE
of 22 March. November 2012
on the protection of experimental animals
299/2014: Sb.
The Ministry of agriculture shall be determined according to § 29 para. 1 of law no 246/1992
Coll., on the protection of animals against cruelty, as amended by Act No. 162/1993 Coll.
Act No. 77/2004 Coll., Act No. 312/2008 Coll., Act No. 291/2009 Coll.
Act No. 359/2012 Coll. (hereinafter the "Act") to implement section 15b paragraph. 1, §
15 c of paragraph 1. 7, § 15 g of paragraph 1. 7, section 16a para. 1, § 17 para. 3, § 17a. 5
(a). b), c) and (d)), section 18 c of paragraph 1. 5 and § 18 g of paragraph 1. 8 of the Act:
§ 1
The subject of the edit
This Decree incorporates the relevant provisions of the European Union ^ 1) and
modifies the
and the pattern of the application form) to grant permission to use the experimental
animals and the model application form for permission to breed test
animals or to the supply of experimental animals,
(b)), the list of data that the breeder of experimental animals, the supplier
experimental animals or user of experimental animals must submit
reviewers, and the content of the written report of assessors,
c) patterns of statistical tables and instructions for filling it out,
d) model application form for approval of the project experiments
(e)) the requirements for premises and equipment, in terms of their function
and the General layout, breeding spaces, spaces for the implementation of the General and
specific experiments, operating facilities, the environment and its requirements
check as regards ventilation and temperature, lighting, noise, alarm
systems, and other requirements for equipment, premises and equipment,
f) requirements relating to the care and placement of test animals, if
This is the State of health of the animals harvested in the wild to the trials,
the location of the experimental animals, environment, design editing closed
space, feeding, watering and construction and equipment for relaxation and
sleep and the handling of laboratory animals,
g) requirements relating to the care and placement in relation to individual species
experimental animals,
h) matching criteria, factors relating to the attempt, and
examples of the various experiments included in each category of severity
on the basis of factors that relate to the type of experiment,
I) methods of killing each of the species of experimental animals,
(j)), the number of test animals when their carriage in the container.
§ 2
A model application form for granting permission to use experimental animals
and the model request form for granting permission to the breeding of experimental animals
or to the supply of experimental animals
(1) the model application form for granting permission to use the experimental
the animal is listed in annex 1 to this notice.
(2) the model application form for permission to breed test animals
or to the supply of experimental animals is given in annex 2 to this
the Decree.
§ 3
The list of data to be submitted and provide the content of the written report
(1) the keeper of experimental animals, a supplier of test animals, or the user
experimental animals, which asks for permission to breed test
animals, permission to supply of experimental animals or permission to use
experimental animals (hereinafter referred to as "the applicant"), reviewers shall submit to the
and the document authorizing the use of) buildings for breeding or delivery of experimental
animals or to conduct experiments on animals by building
law or binding opinion of the regional veterinary service in
land use, construction and kolaudačním proceedings, if requested by other
the law ^ 2)
(b)) a written statement to the competent regional veterinary administration activities
the device,
(c) the operational rules of the device)
d) technological procedures,
(e) documentation on the nature of the objects) of the device, including the address, the exact
the location, name, or other marking each space that
It is used for breeding or supply of experimental animals or in which they are carried out
experiments on experimental animals,
f) organizational chart of the device,
(g)) the list of jobs that can be performed only with a certificate of
professional competence to design experiments and experiments or projects with
certificate of professional competence to conduct experiments on experimental
animals, care of experimental animals and killing of experimental animals,
documented part of the list of employees with a statement of whether the certificate
It was issued,
h) document proving acquisition of relevant higher education
under the law on universities in the event DV
doctor.
(2) if the applicant is applying for permission to breed test animals to
the supply of experimental animals or to the use of experimental animals to the next
the period shall submit to provide permission for the previous period,
In addition to the particulars and documents referred to in paragraph 1,
and documentation)
1. breeding of experimental animals, in the case of breeders of experimental animals or
supplier of experimental animals, and
2. breeding and use of experimental animals, in the case of a user test
animals,
(b)) records for the
1. number of reared, purchased or supplied to experimental animals, if
the experimental animals or experimental animals, with vendors
accompanied by their origin and
2. the number of used, kept or purchased experimental animals, if
about user experimental animals, accompanied by their origin,
(c) the records of the number of dead) experimental animals,
(d) records of checks) the State of health of experimental animals, including
results of laboratory tests,
e) records of the supervisory authorities,
f) projects, including the attempts of documents that have not been approved by the professional
by the Commission for ensuring the welfare of experimental animals or
the national authority responsible for the approval of projects, experiments, if this is
about user experimental animals,
g) documentation on the experimental animals used in relation to the
projects that have taken place from the attempts of the previous assessment, assessors,
If this is about users of experimental animals,
h) information provided to record the animals to the competent authority
for the approval of projects, experiments, if this is about user test
animals,
I) changes to the information of the device or breeder of experimental animals, the vendor
experimental animals or user of experimental animals.
(3) a written opinion in addition to the requirements laid down by the administrative
regulations and requirements set out in section 15b paragraph. 4 of the Act, in particular,
and) the name or names, and surname of the tasters, the number of the certificate of
professional competence to design experiments and projects and experiments
assessor,
(b)) a statement whether the applicant has submitted all the information and documents referred to in
paragraph 1 or in paragraph 2,
(c) the assessment of the dossier submitted), in particular the identified deficiencies and
deficiencies,
(d) assessment of the spatial capacity) and technical equipment
the space in which they are kept supplied and used experimental animals,
in particular, the detected faults and shortcomings,
(e) measures for the immediate) recommended the implementation before a decision to
to grant permissions to the breeding of experimental animals, for supply of experimental animals
or to the use of experimental animals and long-term measures recommended
the character, along with a deadline for their implementation,
f) opinion of the assessors, whether the applicant meets or does not meet the requirements of the
on the breeding of experimental animals, the supply of experimental animals or to use
experimental animals provided for by law and by the Decree; does not satisfy the
the applicant also putting these requirements, shortcomings that are in violation of the
by law or by this Decree,
(g) the name of the Government Department responsible) to approve projects experiments
pursuant to section 23 of the Act, if it is a user of experimental animals,
h) Declaration of the breeders of experimental animals, a vendor of experimental animals
or users of experimental animals, it is with the content of his report, the familiar,
date and signatures of the assessors and the applicant.
§ 4
Patterns of statistical tables, instructions for filling it out, and for more information
that must include a summary report on the activities of the device
Patterns of statistical tables and instructions for filling it out are listed in
Annex No. 3 to this notice.
§ 5
Pattern approval request form project experiments
Pattern approval request form project experiments is given in annex
No 4 to this notice.
§ 6
The requirements for facilities, equipment, and environment requirements
his control and other requirements on the equipment, premises and equipment
(1) the requirements for premises and equipment, in terms of their
the function and the General layout, breeding spaces, spaces for the implementation
General and specific experiments, operating facilities, requirements for the
environment and its control in terms of ventilation, lighting, temperature and
noise, alarm systems, are listed in annex 6 to this Ordinance.
(2) the requirements for the premises, their size and facilities in relation to the
individual species of experimental animals and requirements relating to the care and
the location in relation to individual species of experimental animals are listed in the
Appendix 7 to this Decree.
§ 7
Requirements relating to the care and placement of experimental animals
Requirements relating to the care and placement of test animals, if it is
the health status of the animals harvested in the wild to the trials,
the location of the experimental animals, environment, design editing closed
space, feeding, watering and construction and equipment for relaxation and
sleep and the handling of laboratory animals are listed in annex 8 to
This Decree.
§ 8
Matching criteria attempts to various categories of severity,
factors and examples of different experiments listed in each category
severity based on factors
Matching criteria, factors relating to the attempt, and
examples of the various experiments included in each category of severity
on the basis of factors that relate to a given species, are listed
in Appendix 9 to this Decree.
§ 9
Methods of killing each of the species of experimental animals
Methods of killing each of the species of experimental animals are listed in the
Appendix 10 to this Decree.
§ 10
The number of test animals when their carriage in the container
The number of test animals when their carriage in the container is listed in
Appendix 11 to this Decree.
§ 11
Transitional provision
Until 31 December 2006. December 2016 may breeder of experimental animals, the supplier
experimental animals and the user of experimental animals in spaces
inside the device, that are permanently intended for farming or the use of
experimental animals, breeding size containers or cages, the spatial
the arrangement of the boxes or stalls for selected species of experimental animals by
the existing legislation.
§ 12
Regulation (EEC)
Shall be repealed:
1. Decree No. 207/2004 Coll., on the protection, breeding and use of experimental
animals.
2. Decree No. 39/2009 Coll., amending Decree No. 207/2004 Coll.
the protection, breeding and use of experimental animals.
3. Decree No. 128/2010 Coll. on competence for identification of dogs by tattooing.
section 13 of the
The effectiveness of the
This Decree shall enter into force on 1 January 2000. January 1, 2013.
Minister:
Ing. Sarah b in r.
Annex 1
A model application form for granting permission to use experimental animals
Annex 2
Model request form for granting permission to the breeding of experimental animals
or to the supply of experimental animals
Annex 3
Patterns of statistical tables and instructions for filling it out
Annex 4
Pattern approval request form project experiments
Annex 5
cancelled
Annex 6
Requirements for premises and equipment, in terms of their function and
General layout, breeding spaces, spaces for the implementation of the General and
specific experiments, operating facilities, the environment and its requirements
check as regards ventilation and temperature, lighting, noise, alarm
systems
(1) the keeper of experimental animals, a supplier of test animals, and the user
experimental animals shall ensure that the premises comply with the following
requirements and to be carried out the following activities, if it is a
and function and general arrangement), where
1. all installations and equipment must be so designed as to species
experimental animals, that are placed in them, provide an environment
that takes into account their physiological and behavioural needs. In addition, the
must be dealt with and managed so as to ensure, prior to entering
unauthorized persons, persons who have obvious symptoms of the disease
portable on experimental animals, and against the intrusion of other animals or leakage
experimental animals here located,
2. the devices must be put in the active maintenance programme to ensure the
prevent and remedy all the damages to buildings or equipment,
3. the device must be available and facilities to enable the newly placed
the obtained experimental animals in isolation until their health is not detected
status, and assessed and minimized any risk to your device
living the experimental animals,
4. must be available to separate areas for the location of sick or
injured animals
(b) where the premises)
1. the devices must be a regular and efficient cleaning program
space and maintained in satisfactory hygienic standards
2. walls and floors in the premises shall be provided with a particularly durable
the surface, which is not subject to heavy wear of the experimental animals or
wear of the cleaning work. The material must be for experimental animals
harmless and may not hurt. Tools and equipment must be
particularly protected so that experimental animals could not harm or
They injure itself,
3. nesnášenlivé species of experimental animals, such as the predator and its
potential prey, or experimental animals requiring different life
the conditions must not be placed in the same room and in the case of
predator and prey must be at such a distance to each other
seen, felt or heard,
(c)) for the implementation of space the General and specific experiments, where
1. the laboratory shall be provided with facilities for the implementation of the
simple diagnostic tests, post-mortem examinations or for
sampling, which will be subjected to more thorough laboratory elsewhere
in tests. There, where these operations are carried out, must be available
spaces for the implementation of General and specific experiments,
2. must be available one or more properly equipped rooms and
device for postoperative recovery, if it is necessary to perform a surgical
interventions under sterile conditions,
d) operating areas where
1. storage areas must be handled, used and maintained to
ensure the quality of feed and litter. These premises must be within the
options are protected against insects and other pests. Other materials that
could be contaminated or could pose a risk to
experimental animals or employees, shall be stored separately,
2. There must be space for cleaning and washing so large that to them was
can place a device for cleaning and disinfection of used equipment.
The cleaning process must be carried out so as to clean equipment when handling
without coming into contact with the polluted and freshly cleaned equipment so
protect from contamination,
3. the device must ensure hygienic storage and safe disposal
the bodies of dead animals and animal waste.
(2) environment and its control must meet the following requirements
If this is a
ventilation and temperature), where
1. insulation, heating and ventilation of the room must provide breeding,
that air circulation, dust and gas concentrations are kept in
limits which are not harmful to located the experimental animals,
2. the temperature and relative humidity in the breeding areas must be
adapted to the species of experimental animals and wagons age groups.
The temperature must be measured and logged daily,
3. experimental animals shall not be kept in climatic runs
conditions, which would raise the fear in them,
b) lighting, where
1. in the case that natural daylight does not provide the proper rhythm of the day
and night, this rhythm must be secured with a view to artificial lighting
to satisfy the biological needs of experimental animals and at the same time to ensure
satisfactory working conditions,
2. lighting must meet the needs of the implementation of animal husbandry and
tours of the experimental animals,
3. must be ensured by regular fotoperiodicita and illumination intensity
appropriate for the species of experimental animals,
4. when breeding albino experimental animals must be lighting
adapted to the sensitivity of the test animals to light,
c) noise, where
1. noise levels including ultrasound may not have an adverse effect on good
the welfare of experimental animals,
2. the device must have alarm systems that are set to the frequency
outside the zone, on which the experimental animals are sensitive; in doing so, but it must be
audible to the human ear,
3. premises must be where it is necessary in the interests of good
the living conditions of experimental animals, equipped with noise insulation and
sound-absorbing materials
d) alarm systems, where
1. equipment with electrical or mechanical equipment for the control and
the protection of the environment must have a spare system to ensure basic
services and the emergency lighting and the operation of alarm systems themselves,
2. heating and ventilation systems must be equipped with monitoring devices
and alarm devices,
3. clear instructions for emergency situations shall be visibly placed.
Annex 7
Requirements for the premises, their size and facilities in relation to the
individual species of experimental animals and requirements relating to the care and
the location in relation to individual species of experimental animals
1. Mice, rats, gerbils, hamsters and Guinea Pigs
In the following tables for mice, rats, gerbils, hamsters, and
Guinea Pigs are "enclosure height" means the vertical distance
the space between the floor and ceiling, the height of the space should have
For more than 50% of its minimum floor area before adding devices
to modify the environment. When planning experiments must be taken into account the possible
the growth of experimental animals in order to ensure sufficient space for them (as
It is mentioned in detail in tables 1.1 to 1.5) throughout the study.
Table 1.1
------------------------------------------------------------- ------------------------------------------------
The mouse
Physical minimum area floor area minimum height
the weight of the confined space per animal enclosure
(g) (cm2) (cm2) (cm)
------------------------------------------------------------- ------------------------------------------------
Available in the facility up to 20 330 60 12
and during experiments
from 20 to 25 330 70 12
from 25 to 30 330 80 12
over 30 330 100 12
Reproduction 330 12
For a monogamous pair
(outbrední/inbred)
or three of a kind
(inbred). For
each additional female
and litter add
180 cm2.
------------------------------------------------------------- ------------------------------------------------
Breeding animals ^ 1) less than 20 950 40 12
------------------------------------------------------------- ------------------------------------------------
Area closed
space
950 cm²
The area contained less than 20 1500 30 12
space
1500 cm2
------------------------------------------------------------- ------------------------------------------------
Note:
1) Mouse after weaning can behave at these higher stocking densities in
the short period after weaning to use in experiments, provided that
the animals are placed in large enclosed spaces with a reasonably
the modified environment and location of these conditions do not adversely affect the
animal welfare, for example, do not cause increased aggression,
morbidity and mortality, the development of stereotypií and other behavioral disorders, weight loss
weight or other disruption of physiological functions or behaviour in the
as a result of stress.
Table 1.2
------------------------------------------------------------- ------------------------------------------------
The rats
Body weight minimum area floor area minimum height
(g) an enclosed space per animal enclosure
(cm2) (cm2) (cm)
------------------------------------------------------------- ------------------------------------------------
Available up to 200 800 200 18
in the device, and during
attempts to "^ 1")
from 200 to 300 800 250 18
from 300 to 400 800 350 18
from 400 to 600 800 450 18
over 600 1500 600 18
Reproduction of 800 18
Mother and litter. For
each additional adult
animal located
on a permanent basis to a closed
space is added
400 cm2.
------------------------------------------------------------- ------------------------------------------------
Breeding animals ^ 2) up to 50 1500 100 18
------------------------------------------------------------- ------------------------------------------------
Area closed
space
1500 cm2
from 50 to 100 1500 125 18
from 100 to 150 1500 150 18
from 150 to 200 1500 175 18
------------------------------------------------------------- ------------------------------------------------
Breeding animals ^ 3) up to 100 2500 100 18
------------------------------------------------------------- ------------------------------------------------
Area closed
space
2500 cm2
from 100 to 150 2500 125 18
from 150 to 200 2500 150 18
------------------------------------------------------------- ------------------------------------------------
Notes:
1) in the implementation of long-term studies, if at the end of these studies
the space allocated to each individual experimental animal less than the
mentioned above, is a priority of maintaining a socially stable structures.
2) Rats after weaning can behave at these higher stocking densities
in the short period after weaning to use in experiments, provided that
experimental animals are placed in large enclosed spaces with
appropriately modified by the environment and these terms do not have location
an adverse effect on the welfare of experimental animals, for example,
do not cause increased aggression, morbidity and mortality, the development of
stereotypií and other behavioral disorders, weight loss or other breach
physiological function or behavior as a result of stress.
3) Rats after weaning can behave at these higher stocking densities
in the short period after weaning to use in experiments, provided that
experimental animals are placed in large enclosed spaces with
appropriately modified by the environment and these terms do not have location
an adverse effect on the welfare of experimental animals, for example,
do not cause increased aggression, morbidity and mortality, the development of
stereotypií and other behavioral disorders, weight loss or other breach
physiological function or behavior as a result of stress.
Table 1.3
------------------------------------------------------------- ------------------------------------------------
Gerbils
Minimum Floor area weight minimum height
(g) confined space confined space area
one animal (cm2) (cm)
(cm2)
------------------------------------------------------------- ------------------------------------------------
Available up to 40 1200 150 18
in the device
and during experiments
over 40 1200 250 18
Reproduction 1200 18
Monogamous pair or
Trio
with a child.
------------------------------------------------------------- ------------------------------------------------
Table 1.4
------------------------------------------------------------- ------------------------------------------------
Hamsters
Minimum Floor area weight minimum height
(g) a closed confined space area
space per animal (cm)
(cm2) (cm2)
------------------------------------------------------------- ------------------------------------------------
Available up to 60 800 150 14
in the device
and during experiments
from 60 to 100 800 200 14
over 100 800 250 14
Reproduction 800 14
Mother or
monogamous couple
with a litter of pups.
Breeding less than 60 1500 100 14
animals ^ 1)
------------------------------------------------------------- ------------------------------------------------
Note:
1) Hamsters after weaning can behave at these higher stocking densities
in the short period after weaning to use in experiments, provided that
experimental animals are placed in large enclosed spaces with
appropriately modified by the environment and these terms do not have location
an adverse effect on the welfare of experimental animals, for example,
do not cause increased aggression, morbidity and mortality, the development of
stereotypií and other behavioral disorders, weight loss or other breach
physiological function or behavior as a result of stress.
Table 1.5
------------------------------------------------------------- ------------------------------------------------
Guinea Pigs
Minimum Floor area weight minimum height
(g) a closed confined space area
space per animal (cm)
(cm2) (cm2)
------------------------------------------------------------- ------------------------------------------------
Available up to 200 1800 200 23
in the device
and during experiments
from 200 to 300 1800 350 23
from 300 to 450 1800 500 23
from 450 to 700 2500 700 23
over 700 2500 900 23
Reproduction 2500 23
Pair with litter
the offspring. For
each additional
breeding female is
add 1000 cm2.
------------------------------------------------------------- ------------------------------------------------
2. Rabbits
During agricultural research, in which the aim of the project requires that attempts
experimental animals were kept under similar conditions as the economic
animals kept for commercial purposes, their breeding, at least
comply with the standards referred to in Act No. 246/1992 Coll., on the protection of animals against
cruelty, as amended, and Act No. 166/1999 Coll.
veterinary care and amending certain related laws (health
Act), as amended.
In a confined space must be ensured by the elevated platform. This raised
the platform shall enable to experimental animals to lie and sit and easily under the
hide it and does not cover more than 40% of the floor area. If from
scientific or health reasons cannot be elevated platform used
must be a closed space for rabbits about 33% larger for one rabbit
and about 60% larger for two rabbits. If the raised platform used for
rabbits under ten weeks, its dimensions must be at least 55 cm to 25
cm and must be so high from the floor, so that it could test animals
to take advantage of.
Table 2.1
Rabbits older than 10 weeks
Table 2.1 applies to cages and pens. For every 3rd,
the fourth, fifth and sixth rabbit adds the additional area at least
3000 cm2 and for each additional rabbit area at least 2500 cm2.
------------------------------------------------------------- ---------------------
Terminal body Minimum floor area for one minimum height
weight or two socially compatible animals (cm)
(kg) (cm2)
------------------------------------------------------------- ---------------------
less than 3 3500 45
from 3 to 5 4200 45
over 5 5400 60
------------------------------------------------------------- ---------------------
Table 2.2
Sanjay and litter
------------------------------------------------------------- ---------------------
Weight minimum area Additional Sanjay minimum height
(kg) enclosure space (cm)
(cm2) nest boxes
(cm2)
------------------------------------------------------------- ---------------------
less than 3 3500 1000 45
from 3 to 5 4200 1200 45
over 5 5400 1400 60
------------------------------------------------------------- ---------------------
Table 2.3
Rabbits under ten weeks
Table 2.3 shall apply to the cages and pens.
------------------------------------------------------------- ---------------------
The age of minimum area the minimum minimum height
enclosure floor (cm)
the area (cm2)
one animal
(cm2)
------------------------------------------------------------- ---------------------
up to 7 weeks after 4000 800 40
unset
from 7 to 10 weeks of 4000 1200 40
------------------------------------------------------------- ---------------------
Table 2.4
Rabbits: optimal dimensions of a raised platform in a closed compartment of
the dimensions given in table 2.1.
------------------------------------------------------------- ---------------------
Age (weeks) the final body weight, Optimal, optimal height from floor
(kg) size of the confined space
(cm x cm) (cm)
------------------------------------------------------------- ---------------------
over less than 10 3 55 × 25 25
from 3 to 5 55 × 30 25
over 5 60 × 35 30
------------------------------------------------------------- ---------------------
3. The Cat
Cats must not be placed individually on more than 24 hours without
breaks. Cats that are against other cats showing up repeatedly
aggressively, is placed separately only if it is not possible to find a
of the individual, which can withstand. Social stress for all individuals placed
in pairs or in groups of monitors at least once a week. Females with
koťaty younger than four weeks or the last two weeks of pregnancy
they can be placed separately.
Table 3
Cats
The minimum space for the possession of breeding females and litter is a space reserved for the
one cat that is zoomed so that the chicks into four
months after birth relocated to premises meeting the requirements on
area of adult animals.
The feeding area and the area for the feces must be positioned
at least 0.5 m and these areas must not be confused.
------------------------------------------------------------- -----------
^ 1) floor Cornice Height
(m2) (m2) (m)
------------------------------------------------------------- -----------
Minimum for one 1.5 0.5 2
adult animal
Additional space for 0.75 0.25 —
for each additional animal
------------------------------------------------------------- -----------
Note:
1) floor area without ledges.
4. Dogs
Animals should have, where possible, outdoor enclosures. Dogs must not be placed
individually on more than four hours without a break.
Indoor enclosed space shall occupy at least 50% of the minimum space
reserved for dogs, which is shown in table 4.1.
The parameters below are based on the requirements of the allocated space for
bígly, but for the giant breeds, such as Saint Bernards or Irish
vlkodavy, spaces must be reserved considerably larger than premises specified
in table 4.1. For other breeds, than are laboratory bíglové, must be
the allocated space, consulted with veterinary staff.
Table 4.1
Dogs
Space for each of the dogs placed in pairs or in a group may be in
the course of applied experiments limited to half of the total reserved
space (2 m2 for a dog under 20 kg, 4 m² for a dog over 20 kg), as provided for in the law
or this Ordinance, if this is important for the scientific Department of the animal
purposes. Period during which the space is limited in this way, the dog must not exceed 4
hours without a break.
A nursing female dog and the young must be assigned to the same space as the
by the same weight. Birthing pen has to be designed so that it can
bitch from the puppies away to another part of the space or on elevated
rig.
----------------------------------------------------- -----------------------------------------
Minimum weight the minimum area for each additional minimum height
(kg) contained floor area animal extension (m)
room for one or two at least
(m) animals (m2)
(m2)
----------------------------------------------------- -----------------------------------------
up to 20 4 4 2 2
over 20 8 8 4 2
----------------------------------------------------- -----------------------------------------
Table 4.2
Dogs-weaned puppies
---------------------------------------- ----------------------------------------
The weight of the dog the minimum area the minimum floor minimum height
(kg) enclosure on one area (m)
animal (m2)
(m2)
---------------------------------------- ----------------------------------------
up to 0.5 5 4 2
from 5 to 10 4 2 1.0
from 10 to 15 4 1.5 2
from 15 to 20 4 2 2
nad 20 8 4 2
---------------------------------------- ----------------------------------------
5. Ferrets
Table 5
-------------------------------------- ---------------------------------------------
Ferrets
The minimum area of a minimum floor height is at least
confined space area per one (cm)
animal (cm2)
cm2)
-------------------------------------- ---------------------------------------------
animals up to 600 g 4500 1500 50
animals over 600 g 4500 3000 50
adult males 6000 6000 50
ferret with 5400 5400 50
-------------------------------------- ---------------------------------------------
6. Subhumánní primates
Young subhumánní primates from the mother may separate the age at
six to twelve months, depending on individual species. The environment must
subhumánním're primates make it possible to carry out a complete program daily
activities. Enclosed space must allow in a large well tolerated subhumánním
extent possible, satisfy their Ethological needs and provide them with a sense of
safe and sufficiently diverse environments in which animals can run,
to walk, climb and jump.
Table 6.1
-------------------------------------- ------------------------------------------------
Marmosets and tamaríni
Minimum floor area the minimum volume of minimum height
enclosed space for a single space on ^ 1) every enclosed space
or two animals and offspring to another animal (m) ^ 2)
old age five months earlier of five
(m2) months
(m3)
-------------------------------------- ------------------------------------------------
Marmosets 0.5 0.2 1.5
Tamaríni 1.5 0.2 1.5
-------------------------------------- ------------------------------------------------
In the case of kosmanů and tamarínů with the Cubs from the mother separated by the
the age of eight months.
Notes:
1) animals may be housed individually only in exceptional circumstances.
2) the ceiling of the enclosure shall be at least 1.8 m above the floor.
Table 6.2
------------------------------------------------------
Kotulové
Minimum Minimum minimum volume
floor area of the space on each height
for one ^ 1) or another animal older closed-end
two animals six months of space
(m2) (m3) (m)
-------------------------------------- ---------------
2.0 0.5 1.8
-------------------------------------- ---------------
In the case of squirrel with the Cubs from the mother may separate the age at
six months.
Note:
1) animals may be housed individually only in exceptional circumstances.
Table 6.3
--------------------------------------------------- -------------------------------
Crab-eating monkeys, and ^ 1)
Minimum Minimum Minimum Minimum area
a closed volume volume height
the space of the enclosure to the contained
(m2) of space per animal space
(m3) (m3) (m)
--------------------------------------------------- -------------------------------
Animals less than three 2.0 3.6 1.0 1.8
years ^ 2)
Animals older than three 2.0 3.6 1.8 1.8
years ^ 3)
Animals which are kept for 3.5 2.0
breeding purposes ^ 4)
--------------------------------------------------- -------------------------------
In the case of macaques and marmosets are the Cubs from the mother may separate the first
at the age of eight months.
Notes:
1) animals may be housed individually only in exceptional circumstances.
2) in a confined space with minimum dimensions may be placed
no more than three animals.
3) in a confined space with minimum dimensions may be placed
not more than two animals.
4) is not required in the breeding populations of the additional area/room for
the Cubs within two years along with his mother.
Table 6.4
------------------------------------------------------- ------------------------------------
Baboons ^ 1)
Minimum Minimum Minimum Minimum volume
the volume of space in one area height
closed closed zvířeuzavřeného
compartment (m3)
(m2) (m3) (m)
------------------------------------------------------- ------------------------------------
Animals less than four years ^ 2) 4.0 7.2 3.0 1.8
Animals over four years ^ 3) 7.0 12.6 6.0 1.8
Animals held for breeding 12.0 2.0
the purpose of the ^ 4)
------------------------------------------------------- ------------------------------------
In the case of the baboons, the Cubs from the mother may separate the age at
eight months.
Notes:
1) animals may be housed individually only in exceptional circumstances.
2) in a confined space with minimum dimensions may be placed
not more than two animals.
3) in a confined space with minimum dimensions may be placed
not more than two animals.
4) is not required in the breeding populations of the additional area/room for
the Cubs within two years along with his mother.
7. Farm animals
During agricultural research, in which the aim of the project requires that attempts
experimental animals were kept under similar conditions as the economic
animals kept for commercial purposes, their breeding, at least
comply with the standards referred to in Act No. 246/1992 Coll., on the protection of animals against
cruelty, as amended, and Act No. 166/1999 Coll.
veterinary care and amending certain related laws (health
Act), as amended.
Table 7.1
------------------------------------------------------- ------------------------------------
Bovine animals
Body weight Minimum Minimum space at the trough the space at the trough when
(kg) area of the floor when you adlibitním a restrictive
the closed area to feed the bezrohého feeding bezrohého
space per animal of bovine cattle
(m2) (m2/animal) (m/animal) (m/animal)
------------------------------------------------------- ------------------------------------
up to 100 2.50 2.30 0.10 0.30
from 100 to 200 4.25 3.40 0.15 0.50
from 200 to 400 6.00 4.80 0.18 0.60
from 400 to 600 9.00 7.50 0.21 0.70
from 600 to 800 11.00 8.75 0.24 0.80
over 800 16.00 10.00 0.30 1.00
------------------------------------------------------- ------------------------------------
Table 7.2
-------------------------------------------------------------- ---------------------------------
Ovine and caprine animals
Minimum Minimum Minimum body surface area of the space at the trough
the weight of the contained floor area height in the gutter when you
(kg) of space per animal desk adlibitním a restrictive
(m2) (m2/animal) (m) feeding
(m/animal) (m/animal)
-------------------------------------------------------------- ---------------------------------
less than 20 1.0 0.7 1.0 0.10 0.25
from 20 to 35 1.5 1.0 1.2 0.10 0.30
from 35 to 60 2.0 1.5 1.2 0.12 0.40
over 60 3.0 1.8 1.5 0.12 0.50
-------------------------------------------------------------- ---------------------------------
Table 7.3
-------------------------------------------------------------- ---------------------------------
Pigs and miniprasata
Live weight Minimum Minimum minimum area of a closed area for encampment
(kg) ^ 1) floor area per animal
(2) (vtermoneutrálních area
one animal conditions)
(m2/animal) (m2/animal)
-------------------------------------------------------------- ---------------------------------
up to 5 2.0 0.20 0.10
from 5 to 10 2.0 0.25 0.11
from 10 to 20 2.0 0.35 0.18
from 20 to 30 2.0 0.50 0.24
from 30 to 50 2.0 0.70 0.33
from 50 to 70 3.0 0.80 0.41
from 70 to 100 3.0 1.00 0.53
from 100 to 150 4.0 1.35 0.70
over 150 5.0 2.50 0.95
Adult males (pig 7.5 1.30
Home)
-------------------------------------------------------------- ---------------------------------
Note:
1) Pigs may be for veterinary or experimental reasons for shorter
the period closed in smaller spaces, created such as přepažením
the main space, for example, the individual is required.
Table 7.4.
The equidae
The shortest side is at least half larger than the height of the animal in the
the withers. The height of indoor enclosed space allows the animals to
completely straightened.
-------------------------------------------------------------- ---------------------------------
Height at the Withers, the minimum floor area per animal, the minimum height
(m) (m2/animal) enclosure
(m)
-------------------------------------------- --------------------------
For each animal for each Birth
held by the animal held box/Mare
individually or in groups with foal
Group of four
the number of animals or more
animals
-------------------------------------------------------------- ---------------------------------
from 1.00 to 3.00 16 1.40 9.0 6.0
from 1.40 to 1.60 12.0 9.0 20 3.00
over 1.60 16.0 (2 x height 20 3.00
at the withers) ^ 1)
-------------------------------------------------------------- ---------------------------------
Note:
1) to ensure there is sufficient space must surround the conditions for
each experimental animal be based on its height at the withers.
8. The birds
During agricultural research, in which the aim of the project requires that attempts
experimental animals were kept under similar conditions as the economic
animals kept for commercial purposes, their breeding, at least
comply with the standards referred to in Act No. 246/1992 Coll., on the protection of animals against
cruelty, as amended, and Act No. 166/1999 Coll.
veterinary care and amending certain related laws (health
Act), as amended.
Table 8.1
Chickens
If not for scientific reasons, the following minimum dimensions closed
space to make sure they justify the time of conclusion of the birds in a limited space
the person performing the experiment in consultation with veterinary staff. Under these
circumstances, birds can be housed in smaller confined areas with
the appropriate editing environment and a minimum floor area of 0.75 m2.
-------------------------------------------------------------- ---------------------------------
Body weight Minimum Minimum Minimum minimum area
(g) confined space area at the height of the feeder on the one
(m2) per bird (cm) bird
(m2) (cm)
-------------------------------------------------------------- ---------------------------------
up to 200 1.00 0.025 30 3
from 200 to 300 1.00 0.03 30 3
from 300 to 600 1.00 0.05 40 7
from 600 to 1200 2.00 0.09 50 15
from 1200 to 1800 2.00 0.11 75 15
from 1800 to 2400 2.00 0.13 75 15
over 2400 2.00 0.21 75 15
-------------------------------------------------------------- ---------------------------------
Table 8.2
Turkeys
All parties in the confined space for experimental animals must be long
at least 1.5 m. If not for scientific reasons, the following minimum dimensions
make sure they justify the time of conclusion of the birds in a confined space, the person
conducting the trial in consultation with veterinary staff. Under these
circumstances, birds can be housed in smaller areas with appropriate
by modifying the environment and minimum floor area of 0.75 m2 and a minimum
the height of 50 cm for birds weighing up to 0.6 kg, 75 cm for birds to
weight of 4 kg, and 100 cm for birds weighing more than 4 kg. These
spaces may be used for placement of small groups of birds in accordance with the
the spatial conditions listed in table 8.2.
-------------------------------------------------------------- ---------------------------------
Physical minimum area the minimum minimum height minimum length feeders
the mass of a closed space (cm) area per bird
(kg) (m) a (cm)
Bird
(m2)
-------------------------------------------------------------- ---------------------------------
up to 0.3 2.00 0.13 50 3
from 0.3 to 0.6 2.00 0.17 50 7
from 0.6 to 1 100 15 2.00 0.30
from 1 to 4 2.00 0.35 100 15
from 4 to 8 100 15 2.00 0.40
from 8 to 12 2.00 0.50 150 20
from 12 to 16 2.00 0.55 150 20
from 16 to 20 150 20 2.00 0.60
over 20 150 20 3.00 1.00
-------------------------------------------------------------- ---------------------------------
Table 8.3.
-------------------------------------------------------------- ---------------------------------
Quail
Body on the minimum area of 1 Area to minimum minimum length
weight area bird feeders on the height of each one
(g) the closed positioned next bird (cm) bird
space in a pair of under (cm)
(m2) (m2) location
-------------------------------------------------------------- ---------------------------------
up to 150 1.00 0.5 0.10 20 4
over 150 1.00 0.6 0.15 30 4
-------------------------------------------------------------- ---------------------------------
Table 5.2
Ducks and geese
If not for scientific reasons, the following minimum dimensions closed
space must the time of conclusion of the birds in a limited space
justified by the person making the attempt, in consultation with veterinary staff.
In such circumstances, birds can be housed in smaller enclosed
areas with appropriate editing environment and minimum floor space
0.75 m2. These spaces can be used for placement of small groups
birds in accordance with the spatial conditions listed in table 8.4.
-------------------------------------------------------------- ---------------------------------
Body weight minimum area Area on the minimum minimum length
(g) an enclosed space per bird feeder height on one
(m2) (m2) ^ 1) (cm) bird
(cm)
-------------------------------------------------------------- ---------------------------------
ducks
up to 300 2.00 0.10 50 10
from 300 to 1200 ^ 2) 2.00 0.20 200 10
over 1200 to 3500 2.00 0.25 200 15
over 3500 2.00 0.50 200 15
the geese
up to 500 2.00 0.20 200 10
from 500 to 2000 2.00 0.33 200 15
over 2000 2.00 0.50 200 15
-------------------------------------------------------------- ---------------------------------
Notes:
1) Includes a water tank with a minimum area of 0.5 m2 on 2 m2 closed
space with a minimum depth of 30 cm. water tank can account for up to 50%
the minimum area enclosed space.
2) Immature birds can be kept in enclosed areas with minimal
height 75 cm.
Table 8.5
Ducks and geese: minimum size of water tank ^ 1)
----------------------------------------------------
Area Depth
(m2) (cm)
----------------------------------------------------
ducks 0.5 30
geese 0.5 from 10 to 30
----------------------------------------------------
Note:
1) water tank Dimensions apply to enclosed space covering an area of 2 m2.
The water tank can be up to 50% of the minimum area enclosed space.
Table 8.6.
Pigeons
Enclosed spaces must be rather long and narrow (for example, 2 m x 1 m)
than square to allow birds short flights.
---------------------------------------------------- -----------------------------------------
The minimum area of a minimum group size of Minimum Minimumlength perch
enclosure height length per bird feeders
(m2) (cm) (cm)
Bird
(cm)
---------------------------------------------------- -----------------------------------------
up to 6 2 200 5 30
from 7 to 12 3 200 5 30
for each 0.15 5 30
the next bird
over 12
---------------------------------------------------- -----------------------------------------
Table 5.4
Colorful Zebřičky
Enclosed spaces must be rather long and narrow (for example, 2 m x 1 m)
than square to allow birds short flights. Breeding research
the steam can be put in small venues with appropriate editing environment
minimum floor area of 0.5 m2 and a minimum height of 40 cm. Length of time
the conclusion of the birds in a confined space must justify the person performing the
the attempt, in consultation with veterinary staff.
---------------------------------------------------- -----------------------------------------
Group size the minimum area of the closed-end minimum height of Minimálnípočet feed
area (cm)
(m2)
---------------------------------------------------- -----------------------------------------
up to 6 100 2 1.0
from 7 to 12 1.5 200 2
from 13 to 20 200 3 2.0
for each additional 0.05 1 6 birds
the bird above 20
---------------------------------------------------- -----------------------------------------
9. the Amphibians
Table 9.1
---------------------------------------------------- -----------------------------------------
Water ocasatí
Body length ^ 1) minimum area of water minimum area the minimum depth of water
(cm) of the surface of the water surface (cm)
(cm2) for each additional
an animal in the
under
the location of the
(cm2)
---------------------------------------------------- -----------------------------------------
up to 10262.5 50 13
from 10 to 15 525 110 13
from 15 to 20 875 200 15
from 20 to 30 1837.5 440 15
over 30 3150 800 20
---------------------------------------------------- -----------------------------------------
Note:
1) measured from the muzzle after the cloaca.
Table 5.7
---------------------------------------------------- -----------------------------------------
Water frogs ^ 1)
Body length ^ 2) minimum area of water minimum area the minimum depth of water
(cm) of the surface of the water surface (cm)
(cm2) for each additional
an animal in the
under
the location of the
(cm2)
---------------------------------------------------- -----------------------------------------
less than 6 160 40 6
from 6 to 9 300 75 8
from 9 to 12 600 150 10
over 12 920 230 12.5
---------------------------------------------------- -----------------------------------------
Notes:
1) these terms and conditions apply to tanks in the breeding areas (i.e., when
breeding) rather than the tanks for natural mating and superovulaci because of
performance, when the individual tanks require a smaller size.
Space requirements are established for adults in those
categories of size; young individuals or tadpoles are excluded, or
they are proportionally adjusted to the dimensions.
2) measured from the muzzle after the cloaca.
Table 9.3
---------------------------------------------------- -----------------------------------------
Aquatic frogs
Body length ^ 1) Minimum Minimum minimum height of the area the minimum depth of water
(cm) closed closed area (cm)
space ^ 2) ^ 3 each space)
(cm2) animal in a (cm)
under
the location of the
(cm2)
---------------------------------------------------- -----------------------------------------
up to 1500 200 20 10 5.0
from 5.0 to 7.5 3500 500 30 10
over 7.5 4000 700 30 15
---------------------------------------------------- -----------------------------------------
Notes:
1) measured from the muzzle after the cloaca.
2) one third of the terrestrial part, two-thirds of the water surface is sufficient
for diving animals.
3) measured from the floor up to the inner part of the terrestrial part of the ceiling
the terrarium; In addition, the height of the enclosure shall be adapted to the
its internal layout.
Table 5.8
---------------------------------------------------- -----------------------------------------
Predominantly terrestrial frogs
Body length ^ 1) Minimum Minimum Minimum Minimálníhloubka water area
(cm) of the closed area for height (cm)
space ^ 2) each additional closed
(cm2) an animal in the area ^ 3)
the (cm)
the location of the
(cm2)
---------------------------------------------------- -----------------------------------------
up to 1500 200 20 10 5.0
from 5.0 to 7.5 3500 500 30 10
over 7.5 4000 700 30 15
---------------------------------------------------- -----------------------------------------
Notes:
1) measured from the muzzle after the cloaca.
2) two thirds of the terrestrial part, one-third of the water surface is sufficient
for diving animals.
3) measured from the floor up to the inner part of the terrestrial part of the ceiling
the terrarium; In addition, the height of the enclosure shall be adapted to the
its internal layout.
Table 5.9
------------------------------------ -----------------------------------------------
Tree frogs
Body length ^ 1) minimum area the minimum area minimum height
(cm) enclosure "^ 2") for each additional enclosure ^ 3)
(cm2) animal in a (cm)
under
the location of the
(cm2)
------------------------------------ -----------------------------------------------
up to 3.0 900 100 30
over 1500 200 30 3.0
------------------------------------ -----------------------------------------------
Notes:
1) measured from the muzzle after the cloaca.
2) two thirds of the terrestrial part, one-third of the water surface is sufficient
for diving animals.
3) measured from the floor up to the inner part of the terrestrial part of the ceiling
the terrarium; In addition, the height of the enclosure shall be adapted to the
its internal layout.
10. Reptiles
Table 10.1
------------------------------------ -----------------------------------------------
Water turtles
Body length ^ 1) minimum area the minimum area of the minimum depth of water
(cm) of water surface, water surface (cm)
(cm2) for each additional animal in
the location of the
(cm2)
------------------------------------ -----------------------------------------------
up to 5 600 100 10
from 5 to 10 1600 300 15
from 10 to 15 3500 600 20
from 15 to 20 6000 1200 30
from 20 to 30 10000 2000 35
over 30 20000 5000 40
------------------------------------ -----------------------------------------------
Note:
1) measured in a straight line from the front after the rear edge of the carapace.
Table 10.2
------------------------------------ -----------------------------------------------
Terrestrial snakes
"^ 1" in length) minimum Minimum Minimum area height
(cm) floor area for each additional animal in uzavřenéhoprostoru ^ 2)
(cm2) the location (cm)
(cm2)
------------------------------------ -----------------------------------------------
up to 30 300 150 10
from 30 to 40 400 200 12
from 40 to 50 600 300 15
from 50 to 75 1200 600 20
over 75 2500 1200 28
------------------------------------ -----------------------------------------------
Notes:
1) measured from the muzzle after tail.
2) measured from the floor up to the inner part of the terrestrial part of the ceiling
the terrarium; In addition, the height of the enclosure shall be adapted to the
its internal layout.
11. fish
11.1 the supply and quality of water
Constantly must be ensured adequate water supply of suitable quality.
Water flow in recirculating systems or filtration in the tanks must
to be sufficient and to ensure compliance with water quality parameters on
acceptable levels. The supply water must be filtered or modified.
so that, where appropriate, substances which are removed for fish
dangerous. Parameters of water quality must be kept within an acceptable
the range, which for a given type of fish in a given stage of development, maintains the normal
activity and physiology. The water flow must be sufficient to ensure that
fish allowed to swim properly and behave as usual. Fish
You must have enough time for acclimatization and adaptation to changes
conditions as to the quality of the water.
11.2 oxygen, nitrogen compounds, pH and salinity
Oxygen concentration must match the needs of each species of fish and
the conditions in which fish are kept. If necessary, must be
ensures additional aeration of the water in the tanks. The concentration of
compounds of nitrogen must be kept at lower levels.
The pH level must be adapted to the individual species of fish and maintained
If possible, stable. Salinity must be adapted to the requirements of the species
the fish and the stage of individuals. Changes of salinity must be carried out gradually.
11.3 Temperature, lighting, noise
The temperature must be maintained within the optimal range for the species of fish and
as far as possible on a stable value. Temperature changes must be carried out
gradually. The fish must be ensured by appropriate fotoperiodicita. The levels of
noise must be kept to a minimum and the equipment causing the noise or
vibration, such as electrical generators or filter systems must
be where appropriate, separated from the tanks in which are kept
the fish.
11.4. Stocking density and diversity of the environment
The stocking density of the fish must be based on the overall needs of the fish when it comes to
environmental conditions, health status and well-being. Must be
ensure a sufficient volume of water to keep the fish could swim in the normal way,
taking into account the size, age, State of health, and how
the feeding of the fish. The environment of the fish must be suitably modified, for example,
shelters must be provided, and substrate on the bottom, except for cases where the of the
their behaviour patterns shows that do not.
11.5. Feeding and handling
The fish must be fed a diet that is suitable for them, in the appropriate
quantities and intervals. Special attention should be paid to the feeding
FRY during each transition from natural food on plastic. Handling
with the fish must be kept to a minimum.
Annex 8
Requirements relating to the care and placement of test animals, if it is
the health status of the animals harvested in the wild to the trials,
the location of the experimental animals, environment, design editing closed
space, feeding, watering and construction and equipment for relaxation and
sleep and the handling of laboratory animals
Care of experimental animals and their location shall meet the following
requirements, if it is a
and health, where)
1. in all facilities must be put in place strategies for maintaining
the State of health of experimental animals, which makes them good
living conditions and corresponds to the scientific requirements. This strategy must
include regular monitoring of the State of health of experimental animals,
microbiological checks and program plans to address health problems
experimental animals and shall define health parameters and procedures for the
the adoption of new experimental animals, and
2. tours of the experimental animals must be carried out by a person competent to
conduct experiments on experimental animals, experimental animals and care
the killing of experimental animals at least once a day. These tours must
ensure that this person finds all sick or injured in experimental
animals and shall take the appropriate measures to remedy the condition
(b)) experimental animals harvested in the wild, where
1. at the point of capture must be available for shipping containers and
means of transport adapted to the species of experimental animals for
any transport experimental animals to examinations or treatment, and
2. particular attention must be paid to habituation, quarantine, the location of the
and the breeding of experimental animals caught in the wild and caring for them and
where appropriate, the preparation of their release into the wild after the end of the
and for these situations must be provided conditions suitable for the
a kind of experimental animals,
(c)) the location of the experimental animals, in addition to the solitary by nature experimental
animal experimental animals must be placed in a socially stable
groups of individuals who are snášenliví to each other. In cases where the
permitted State authority competent to approve the projects at
the decision approving the project attempts an individual location, must be
the length of this location as much as possible and in its course must be
experimental animal maintained by Visual, auditory, olfactory, or touch
contact. Location, or the return of the experimental animals into established groups
must be carefully monitored, the avoidance of problems caused by
intolerance and not to disrupt social relationships,
(d) environment, all editing) to experimental animals must be secured
diverse enough space that they extensively will allow common
behavior. Within their environment, they must be granted a certain degree of
control and choice, in order to reduce symptoms of stress-induced behavior.
The device must apply suitable procedures for editing environment that
extend the range of potential activities of experimental animals and promote their
adapting situation, including the body of the movement, getting food, playing
and cognitive activities, according to the species of experimental animals. Editing environment
in confined spaces for experimental animals must be adapted to the
the species of experimental animals and to the individual needs of the
experimental animals. Strategy in the field of editing environment must be in
devices subject to the review and updated regularly,
e) enclosed spaces for experimental animals, shall not be produced from
materials that are harmful to the health of experimental animals. Must be
designed and constructed in such a way that the experimental animals could not injure themselves.
If they are not intended for single use, it must be of resistant
the material, which is not harmful to cleaning and disinfection. Floor closed
space must be adapted to the species of experimental animals and their
old age and to be so designed as to facilitate the removal of excreta,
f) feeding, where
1. the form, the content and the presentation of feed shall conform to the nutritional and
physiological needs of the test animal,
2. feed the experimental animals must be tasty and uncontaminated. When
selection of raw materials for animal feed and in the production, preparation and presentation of feed shall
be taken in order to minimize the risk of chemical,
physical and microbial contamination,
3. feed must be packaged, transported and stored, so that the
avoid contamination, deterioration or destruction. All hoppers,
troughs and other tools to be used for feeding must be cleaned regularly and
If necessary, sterilised
4. protect every animal must be ensured access to the food with the
adequate space to reduce competition,
g) power, where
1. with regard to the physiological needs of the species must have the experimental animals
still available to water that does not endanger the GMO-their
the State of health,
2. must be regularly inspected, maintained and proplachovány,
If automatic power systems by preventing
accidents. If they are used the cage with a fixed date, care must be taken to
minimize the risk of flooding,
3. it must be ensured that the water supplied to the aquariums and tanks was
adapted to the needs and boundaries of tolerance of each species,
Amphibians and reptiles
h) area for rest and sleep, where
1. experimental animals must be constantly available litter or
construction for sleeping, that are tailored to the needs of the species concerned
experimental animals, including materials or structures for building nests in the
breeding of experimental animals, and
2. in confined spaces for experimental animals must be according to the needs
each species of experimental animals provided a solid and comfortable area
for relaxation. All spaces for sleep must be kept clean and
dry,
I) handling, where on the device must be put in place programs to habituation and
training adapted to experimental animals, experiments, and the duration of the project
attempts.
Annex 9
Matching criteria, factors relating to the attempt, and
examples of the various experiments included in each category of severity
on the basis of factors relating to the kind you try to
(1) the assignment of severity category must
and take into account all interventions on) experimental animals or handling
them within a defined procedure
(b)) based on the most serious effects of each experimental animals
You may feel after using all relevant mitigation procedures
(c) take into account the type of attempt and) the factors referred to in paragraphs 2 and 3, all
These factors must be assessed in each individual case.
(2) the factors relating to the attempt to include
and the kind of treatment and handling) with experimental animals,
(b)) the nature of pain, suffering, distress or lasting harm of the experimental
the animal as a result of all the elements of the attempt and its intensity, duration,
the frequency and diversity of the methods used,
c) cumulative suffering within test animal,
d) guard in the speeches of the natural behavior of the test animal, including
restrictions with regard to the location and standards of care.
(3) for the purposes of final severity classification attempts must take into account and
These additional factors shall be assessed for each individual case,
and) the type of experimental animals and genotype,
b) adulthood, age and sex of the test animal,
(c)) navyklost test animal on any given attempt,
(d)) in the case of experimental animal use again, the actual
the severity of previous attempts,
e) methods used to mitigate or eliminate pain, suffering and distress
test animal, including the refinement in the location, breeding and
care,
f) terms the humane treatment of experimental animal.
(4) the Mild kinds of experiments include
and numbness) except in the case where the sole purpose of
the killing of the experimental animal,
b) pharmacokinetic studies, when the protect the animal served only one
dose and removes a limited number of blood samples, that is, the total amount of
less than 10% of the volume in circulation and it is not expected that the substance causes
detectable adverse effect,
(c)) non-invasive imaging of experimental animals, such as MRI, using
the appropriate sedatives or pain-reducing substances,
d) surface, such as a biopsy of the attempts of the ear and tail, non-surgical
subcutaneous minipump implantation and transponders,
e) using external telemetry devices that test animals
cause only slight injury or interfere with normal activities
and behavior,
(f) administration of substances), intramuscular, Intraperitoneal, subcutaneous route
through the probe and intravenously via superficial blood vessels, when
substance has on experimental animal larger than a slight impact and volume is within the
appropriate limits for the size and type of test animal,
g) induction of tumors, or spontaneous tumors, that cause no detectable
Clinical adverse effects of protect animal, such as a small, subcutaneous,
non-invasive nodules,
h) breeding genetically altered experimental animals, which are expected to
the emergence of a phenotype with mild effects,
I) feeding test animal modified food which does not meet all
the nutritional needs of the experimental animal, which, it is expected that in the course of
the study will cause mild clinical abnormalities,
j) short-term, i.e., less than 24 hours of the conclusion of the trial,
animal metabolic cage
the study, which included) is a short-term separation of test animal
from the social partners or short-term separate location of adult
rats or mice with social tendencies in cages,
l) models that expose the experimental animals harmful stimuli that are
briefly associated with slight to moderate pain, suffering or fear, and
establishing experimental animals can successfully avoid,
m) on the basis of a combination or accumulation of the following examples can be
experiments classified as mild
1. assessing body composition test animal with the help of non-invasive
methods and minimum restrictions,
2. monitoring of the ECG test animal with non-invasive methods for minimum
limitation of přivyklých experimental animals or without limitation,
3. use of external telemetry devices, which are not expected to
No injury caused by socially adapted to experimental animals or intervention
in a regular activity and the behavior of the test animal,
4. the breeding of genetically altered experimental animals, which are not expected to
the occurrence of any clinically identifiable Harmful phenotypes,
5. Add inert markers to food animals for the purpose of
tracking the passage of feces,
6. withdrawal of food adult rats for a period not exceeding 24 hours,
7. tests of an open field.
(5) medium kinds of experiments include
and the frequent use of test substances), which produces medium-intense
clinical effects of, and the collection of blood samples, which represent less than 10
% of the volume in circulation in experimental animal conscious within a few days without
filling,
b) studies to determine the extent of the effective dose or the test
chronic toxicity and carcinogenicity, which does not stop killing trial
of the animal,
c) surgical procedures in the overall numbness, and using appropriate
funds reduce the pain associated with pain after surgery, suffering
or the deterioration of the general condition of the experimental animal, in particular torakotomie,
craniotomy, laparotomy, orchidectomy, lymphadenectomy,
tyroidektomie, orthopaedic surgery with effective stabilisation and treatment
wounds, organ transplantation with effective managing rejection, surgical
the introduction of catheters or the use of biomedical devices, such as
telemetry transmitters, minipump,
(d)) ways invoking tumours or spontaneous tumors, which are expected,
that will cause moderate to intense pain or fear or moderate
interference with the normal behavior of the test animal,
e) irradiation of test animal or chemotherapy using sublethal
benefits or otherwise lethal dose, but during the reconstitution of the immune
the system. The expected adverse effects should be mild or moderate and
short-term, i.e., lasting less than 5 days,
f) breeding genetically altered experimental animals, which are expected to
the emergence of a phenotype with medium effects,
(g) the creation of a genetically modified) test animal on the basis of
surgical procedures,
(h)), thus lasting up to 5 days, the use of metabolic cages
with moderate restriction of movement of the test animal,
I) study using a modified food which does not meet all the nutritional
the needs of the test animal, which, it is expected that in the course of the study
causes the animal to protect moderate clinical abnormalities,
j) deprivation of food adult rats for a period of 48 hours,
to escape or evasion) calling the reaction in a situation where the experimental animal
the initiative cannot escape or avoid it, and when it is expected to be moderate
intense fear.
(6) Serious kinds of experiments include
a) toxicity tests, which lead to the death or which can be expected
the death of test animal or the emergence of a serious patofyziologického for status
test animal, such as acute toxicity test after the application of a single
benefits,
(b) testing machines) failure can cause a great deal of pain,
fear or death test animal, such as equipment that supports
heart activity,
(c)) to test the effectiveness of vaccines that are accompanied by the persistent deterioration of the State of
the experimental animal experimental disease leading to death, associated with the
long-term moderately intense pain, fear or suffering,
d) irradiation of test animal or chemotherapy using lethal
dose without reconstitution of the immune system or reconstitution
inducing graft,
e) ways of invoking the tumours or with the emergence of spontaneous tumors, for which
It is expected that causes a progressive fatal disease of test animal
accompanied by long-lasting moderate to intense pain, fear or
suffering, such as tumors causing cachexia, invasive bone
tumors, tumors leading to the spread of metastases and tumors, which allows
ulceration,
f) surgical and other interventions on an experimental animal in the overall
anesthesia, which are expected to cause significant or permanent medium
intensive post-operative pain, suffering or distress or serious and permanent
the deterioration of the general condition of the test animal, causing unstable
fractures or torakotomie test animal without a reasonable reduction in the
pain or trauma in order to raise multiple organ failure
test animal,
g) transplant authority, where it is expected that rejection will lead to institution
significant distress or deterioration of the general condition of the test animal,
for example, on Xeno-transplantation,
h) test animal breeding with genetic disorders, which are expected to
the experimental animal will affect serious and permanent deterioration of the general condition,
for example, Huntington's disease, muscular dystrophy, chronic
recurrent inflammation of the nerves,
and the longer the use of metabolic cages) with severe restrictions of movement
test animal,
j) electric shocks, before whom the experimental animal cannot escape, such as
in order to trigger a studied helplessness,
for the longer term social isolation) full of species of experimental animals,
for example, dogs and primates, subhumánních
l) immobilization stress in rats in order to cause stomach ulcers or
heart failure,
m) tests using forced swimming or exercise, which ends by exhaustion
the experimental animal.
Annex 10
Methods of killing each of the species of experimental animals
Requirements:
(1) where appropriate, the prior administration of sedatives.
(2) this applies only for large reptiles.
(3) the gas is allowed into the Chamber gradually. Not applicable for fruit and
neonatal rodents.
(4) it shall apply only in birds weighing less than 1 kg. Birds with
mass exceeding 250 g serves a sedative.
(5) it shall apply only in rodents, weighing less than 1 kg. Rodents with
weighing more than 150 g serves a sedative.
(6) it's the only rabbits weighing less than 1 kg. Rabbits with
weighing more than 150 g serves a sedative.
(7) it shall apply only in birds weighing less than 5 kg.
(8) it shall apply only in rodents, weighing less than 1 kg.
(9) applies only in rabbits weighing less than 5 kg.
(10) applies only in newborn individuals.
(11) applies only in birds weighing less than 250 g.
(12) it shall apply only if it is not possible using other methods.
(13) special equipment is required.
(14) It is only for pigs.
(15) only performs an experienced shooter in the field.
(16) only performs an experienced shooter in the field, if it is not possible to use
the other method.
Explanatory notes:
1) if the box is empty, the method of killing for a single species
experimental animals allowed.
2) if section proškrtnutá is a method of killing for a single species
experimental animals is prohibited.
3) if section a link to the request, the method of killing for
a single species of experimental animals allowed and is carried out in accordance with the
the relevant requirement.
Annex 11
The number of test animals when their carriage in the container
The number of test animals when their carriage in the container must
take into account the requirements laid down by Council Regulation (EC) No 1/2005 of the day
December 22, 2004 on the protection of animals běhme transport and souvisejícíhc
operations and amending Directives 64/432/EEC and 93/119/EC and Regulation (EC) No.
1255/97, as amended.
1) European Parliament and Council Directive 2010/63/EU of 22 December 2004. September
2010 for the protection of animals used for scientific purposes.
2) Act 166/1999 Coll., on health care and on amendments to certain
related laws (health law), as amended.
